CHEV - BACSU - GREMLIN Contacts

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CHEV - Chemotaxis protein CheV

UniProt:	P37599	Sequences: MSLQQYEILLDSGTNELEIVKFGVGENAFGINVMKVREIIQPVEVTSVPHSHQHVEGMIKLRGEILPVISLFSFFGVEPEGSKDEKYIVTEFNKRKIVFHVGSVSQIHRVSWEAIEKPTSLNQGMERHLTGIIKLEDLMIFLPDYEKIIYDIESDSGVDTYNMHTEGFDERRTDKKLIIVEDSPLLMRLLQDELKEAGYNNIASFENGKEAYEYIMNLAENETDLSKQIDMIITDIEMPKMDGHRLTKLLKENPKSSDVPVMIFSSLITDDLRHRGEVVGADEQISKPEISDLIKKVDTYVIE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	303 (281)
Sequences:	29279 (23827)
Seq/√Len:	1421.4

ECOLI - TORS ARCB BARA EVGS RCSC RCSD
BACSU - CHEV

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
203_A	229_I	3.228	1.00
187_M	204_S	3.085	1.00
140_I	152_I	2.666	1.00
216_M	253_N	2.646	1.00
144_D	147_K	2.638	1.00
207_N	240_K	2.602	1.00
104_V	141_F	2.583	1.00
209_K	240_K	2.572	1.00
130_T	134_K	2.490	1.00
206_E	210_E	2.479	1.00
234_T	246_L	2.472	1.00
93_N	150_Y	2.455	1.00
191_Q	204_S	2.409	1.00
100_H	103_S	2.391	1.00
176_K	200_N	2.364	1.00
180_V	211_A	2.318	1.00
176_K	203_A	2.304	1.00
283_E	296_K	2.270	1.00
89_V	154_S	2.232	1.00
8_I	12_S	2.207	1.00
187_M	206_E	2.201	1.00
242_D	245_R	2.190	1.00
91_E	150_Y	2.175	1.00
197_A	294_I	2.171	1.00
89_V	152_I	2.125	1.00
247_T	261_V	2.075	1.00
69_I	95_R	2.057	1.00
87_Y	154_S	2.055	1.00
251_K	282_D	2.040	1.00
249_L	252_E	2.037	1.00
203_A	214_Y	1.982	1.00
50_H	136_E	1.932	1.00
205_F	214_Y	1.916	1.00
263_I	281_A	1.851	1.00
91_E	152_I	1.839	1.00
247_T	281_A	1.838	1.00
100_H	145_Y	1.832	1.00
99_F	103_S	1.793	1.00
103_S	106_Q	1.754	1.00
142_L	150_Y	1.749	1.00
129_L	139_M	1.737	1.00
191_Q	195_K	1.734	1.00
241_M	249_L	1.717	1.00
16_E	20_V	1.713	1.00
8_I	11_D	1.711	1.00
101_V	104_V	1.705	1.00
237_E	267_L	1.652	1.00
283_E	299_T	1.618	1.00
207_N	210_E	1.614	1.00
172_R	199_Y	1.591	1.00
134_K	138_L	1.589	1.00
209_K	213_E	1.581	1.00
178_I	205_F	1.569	1.00
212_Y	246_L	1.523	1.00
175_K	199_Y	1.496	1.00
106_Q	112_W	1.489	1.00
203_A	227_K	1.488	1.00
262_M	296_K	1.468	1.00
215_I	250_L	1.467	1.00
296_K	299_T	1.467	1.00
274_H	278_V	1.457	1.00
141_F	151_D	1.456	1.00
254_P	257_S	1.451	1.00
241_M	245_R	1.448	1.00
289_E	292_D	1.446	1.00
214_Y	217_N	1.414	1.00
109_R	112_W	1.405	1.00
212_Y	249_L	1.399	1.00
179_I	190_L	1.396	1.00
236_I	244_H	1.395	1.00
73_S	95_R	1.395	1.00
214_Y	229_I	1.391	1.00
234_T	247_T	1.390	1.00
99_F	107_I	1.384	1.00
236_I	263_I	1.375	1.00
254_P	258_D	1.368	1.00
104_V	143_P	1.359	1.00
177_L	231_M	1.358	1.00
76_G	93_N	1.355	1.00
208_G	241_M	1.349	1.00
148_I	154_S	1.341	1.00
249_L	253_N	1.341	1.00
45_V	196_E	1.340	1.00
231_M	297_V	1.339	1.00
193_E	290_I	1.332	1.00
208_G	246_L	1.331	1.00
80_E	89_V	1.324	1.00
142_L	152_I	1.308	1.00
177_L	199_Y	1.297	1.00
245_R	249_L	1.291	1.00
176_K	230_D	1.288	1.00
57_G	129_L	1.282	1.00
94_K	98_V	1.280	1.00
90_T	153_E	1.273	1.00
181_E	187_M	1.270	1.00
176_K	227_K	1.259	1.00
50_H	55_V	1.239	1.00
133_I	153_E	1.238	1.00
57_G	132_I	1.221	1.00
57_G	133_I	1.218	1.00
10_L	13_G	1.218	1.00
244_H	276_G	1.217	1.00
78_E	91_E	1.210	1.00
138_L	154_S	1.204	1.00
232_I	250_L	1.196	1.00
108_H	139_M	1.196	1.00
232_I	259_V	1.195	1.00
15_N	19_I	1.190	1.00
103_S	114_A	1.187	1.00
213_E	217_N	1.178	1.00
45_V	290_I	1.168	1.00
272_L	275_R	1.162	1.00
245_R	248_K	1.161	1.00
149_I	154_S	1.147	1.00
292_D	296_K	1.142	1.00
179_I	202_I	1.131	1.00
58_M	90_T	1.124	1.00
273_R	277_E	1.121	1.00
184_P	187_M	1.113	1.00
176_K	229_I	1.112	1.00
57_G	153_E	1.109	1.00
106_Q	109_R	1.108	1.00
69_I	75_F	1.102	1.00
175_K	230_D	1.094	1.00
270_D	274_H	1.087	1.00
209_K	241_M	1.086	1.00
143_P	147_K	1.086	1.00
213_E	216_M	1.086	1.00
12_S	15_N	1.085	1.00
253_N	256_S	1.083	1.00
292_D	295_K	1.082	1.00
267_L	272_L	1.080	1.00
35_K	39_I	1.079	1.00
188_R	191_Q	1.063	1.00
205_F	211_A	1.062	1.00
110_V	131_G	1.061	1.00
9_L	13_G	1.060	1.00
236_I	276_G	1.058	1.00
210_E	213_E	1.055	1.00
174_D	200_N	1.048	1.00
39_I	189_L	1.046	1.00
269_T	272_L	1.042	1.00
118_P	121_L	1.036	1.00
212_Y	241_M	1.032	1.00
55_V	90_T	1.031	1.00
35_K	185_L	1.021	1.00
178_I	211_A	1.017	1.00
265_S	284_Q	1.015	1.00
69_I	73_S	1.010	1.00
107_I	141_F	1.009	1.00
234_T	263_I	1.009	1.00
129_L	133_I	1.004	1.00
172_R	175_K	1.004	1.00
191_Q	202_I	1.002	1.00
40_I	44_E	0.996	1.00
276_G	284_Q	0.996	1.00
184_P	206_E	0.994	1.00
15_N	18_E	0.985	1.00
41_Q	290_I	0.985	1.00
210_E	214_Y	0.985	1.00
73_S	93_N	0.980	1.00
215_I	259_V	0.977	1.00
114_A	118_P	0.972	1.00
54_H	132_I	0.969	1.00
268_I	286_S	0.968	1.00
180_V	246_L	0.966	1.00
256_S	259_V	0.965	1.00
232_I	261_V	0.964	1.00
211_A	246_L	0.951	1.00
34_M	186_L	0.949	1.00
190_L	264_F	0.948	1.00
290_I	294_I	0.944	1.00
174_D	199_Y	0.940	1.00
215_I	256_S	0.933	1.00
233_I	264_F	0.931	1.00
270_D	273_R	0.930	1.00
74_F	95_R	0.926	1.00
70_S	74_F	0.926	1.00
133_I	139_M	0.921	1.00
143_P	149_I	0.910	1.00
276_G	280_G	0.909	1.00
69_I	72_F	0.904	1.00
130_T	138_L	0.903	1.00
140_I	154_S	0.901	1.00
12_S	16_E	0.896	1.00
99_F	104_V	0.895	1.00
76_G	91_E	0.892	1.00
205_F	210_E	0.892	1.00
236_I	281_A	0.888	1.00
20_V	110_V	0.887	1.00
51_S	54_H	0.887	1.00
184_P	188_R	0.886	1.00
13_G	17_L	0.880	1.00
101_V	144_D	0.875	1.00
215_I	254_P	0.863	1.00
103_S	112_W	0.858	1.00
244_H	275_R	0.857	1.00
62_R	75_F	0.855	1.00
276_G	281_A	0.853	1.00
41_Q	289_E	0.848	1.00
200_N	203_A	0.847	1.00
182_D	238_M	0.840	1.00
215_I	253_N	0.838	1.00
254_P	259_V	0.838	1.00
131_G	135_L	0.837	1.00
38_E	189_L	0.837	1.00
214_Y	227_K	0.834	1.00
143_P	146_E	0.828	1.00
180_V	208_G	0.828	1.00
9_L	12_S	0.828	1.00
248_K	252_E	0.827	1.00
178_I	229_I	0.824	1.00
182_D	235_D	0.822	1.00
246_L	250_L	0.819	1.00
294_I	298_D	0.809	1.00
275_R	278_V	0.805	1.00
99_F	105_S	0.803	1.00
94_K	148_I	0.803	1.00
216_M	249_L	0.802	1.00
181_E	238_M	0.802	1.00
234_T	261_V	0.801	1.00
196_E	290_I	0.800	1.00
233_I	293_L	0.798	1.00
262_M	283_E	0.794	1.00
188_R	192_D	0.794	1.00
131_G	134_K	0.794	1.00
56_E	132_I	0.792	1.00
46_T	196_E	0.789	1.00
99_F	141_F	0.788	1.00
197_A	290_I	0.786	1.00
13_G	16_E	0.783	1.00
211_A	215_I	0.781	1.00
113_E	121_L	0.780	1.00
192_D	195_K	0.779	1.00
61_L	153_E	0.776	1.00
58_M	62_R	0.764	1.00
186_L	189_L	0.762	1.00
195_K	202_I	0.760	1.00
180_V	238_M	0.760	1.00
180_V	234_T	0.759	1.00
215_I	232_I	0.746	1.00
97_I	121_L	0.744	1.00
180_V	232_I	0.741	1.00
89_V	140_I	0.737	1.00
248_K	279_V	0.737	1.00
183_S	186_L	0.737	1.00
48_V	86_K	0.736	1.00
36_V	40_I	0.734	1.00
176_K	228_Q	0.732	1.00
87_Y	138_L	0.730	1.00
291_S	295_K	0.729	1.00
180_V	205_F	0.728	1.00
20_V	24_V	0.728	1.00
295_K	299_T	0.727	1.00
241_M	246_L	0.725	1.00
212_Y	216_M	0.724	1.00
70_S	97_I	0.724	1.00
260_P	282_D	0.723	1.00
244_H	248_K	0.720	1.00
199_Y	202_I	0.719	1.00
271_D	274_H	0.713	1.00
108_H	141_F	0.713	1.00
212_Y	215_I	0.712	1.00
27_N	31_I	0.709	1.00
234_T	250_L	0.708	1.00
209_K	249_L	0.707	1.00
208_G	212_Y	0.706	1.00
14_T	18_E	0.705	1.00
210_E	240_K	0.704	1.00
247_T	263_I	0.703	1.00
251_K	259_V	0.699	1.00
182_D	239_P	0.699	1.00
175_K	298_D	0.699	1.00
61_L	92_F	0.699	1.00
244_H	263_I	0.696	1.00
104_V	142_L	0.695	1.00
199_Y	294_I	0.690	1.00
189_L	193_E	0.684	1.00
24_V	110_V	0.684	1.00
238_M	243_G	0.683	1.00
68_V	113_E	0.683	1.00
258_D	282_D	0.682	1.00
277_E	280_G	0.681	1.00
233_I	262_M	0.680	1.00
61_L	139_M	0.680	1.00
217_N	227_K	0.679	1.00
238_M	246_L	0.679	1.00
100_H	114_A	0.679	1.00
194_L	297_V	0.678	1.00
21_K	25_G	0.678	1.00
188_R	204_S	0.677	1.00
41_Q	45_V	0.671	1.00
99_F	149_I	0.669	1.00
132_I	136_E	0.669	1.00
232_I	246_L	0.664	1.00
49_P	158_V	0.663	1.00
87_Y	156_S	0.663	1.00
273_R	284_Q	0.661	1.00
60_K	63_G	0.659	1.00
38_E	42_P	0.659	1.00
11_D	15_N	0.659	1.00
59_I	63_G	0.656	1.00
177_L	194_L	0.656	1.00
110_V	127_R	0.654	1.00
200_N	227_K	0.652	1.00
133_I	155_D	0.650	1.00
28_A	31_I	0.650	1.00
189_L	192_D	0.648	1.00
240_K	246_L	0.648	1.00
31_I	38_E	0.645	1.00
236_I	267_L	0.644	1.00
207_N	238_M	0.641	1.00
231_M	262_M	0.641	1.00
11_D	14_T	0.641	1.00
277_E	281_A	0.640	1.00
261_V	281_A	0.637	1.00
35_K	38_E	0.637	1.00
236_I	242_D	0.634	1.00
78_E	93_N	0.632	1.00
157_G	160_T	0.628	1.00
247_T	279_V	0.627	1.00
275_R	279_V	0.627	1.00
20_V	116_E	0.626	1.00
182_D	265_S	0.624	1.00
244_H	279_V	0.623	1.00
151_D	154_S	0.622	1.00
61_L	129_L	0.619	1.00
156_S	159_D	0.615	1.00
107_I	127_R	0.613	1.00
78_E	89_V	0.612	1.00
215_I	229_I	0.612	1.00
285_I	296_K	0.610	1.00
208_G	239_P	0.609	1.00
271_D	275_R	0.608	1.00
208_G	238_M	0.608	1.00
116_E	119_T	0.607	1.00
179_I	204_S	0.606	1.00
29_F	32_N	0.606	1.00
194_L	199_Y	0.606	1.00
115_I	118_P	0.605	1.00
14_T	17_L	0.603	1.00
79_P	86_K	0.602	1.00
187_M	191_Q	0.601	1.00
205_F	208_G	0.600	1.00
37_R	40_I	0.600	1.00
250_L	256_S	0.599	1.00
115_I	120_S	0.598	1.00
42_P	192_D	0.592	1.00
235_D	287_K	0.592	1.00
158_V	161_Y	0.591	1.00
248_K	275_R	0.591	1.00
113_E	122_N	0.589	1.00
263_I	276_G	0.585	1.00
182_D	243_G	0.584	1.00
190_L	293_L	0.583	1.00
182_D	287_K	0.583	1.00
273_R	283_E	0.582	1.00
19_I	23_G	0.580	1.00
99_F	143_P	0.579	1.00
179_I	187_M	0.579	1.00
31_I	35_K	0.579	1.00
39_I	42_P	0.578	1.00
184_P	204_S	0.578	1.00
115_I	119_T	0.577	1.00
28_A	32_N	0.575	1.00
31_I	34_M	0.574	1.00
182_D	266_S	0.571	1.00
82_S	85_E	0.568	1.00
192_D	196_E	0.565	1.00
63_G	67_P	0.564	1.00
40_I	43_V	0.561	1.00
208_G	243_G	0.561	1.00
22_F	25_G	0.560	1.00
173_T	199_Y	0.560	1.00
177_L	202_I	0.558	1.00
81_G	86_K	0.558	1.00
190_L	193_E	0.555	1.00
100_H	104_V	0.554	1.00
72_F	75_F	0.552	1.00
27_N	35_K	0.551	1.00
88_I	157_G	0.550	1.00
27_N	30_G	0.548	1.00
178_I	215_I	0.547	1.00
9_L	16_E	0.547	1.00
251_K	254_P	0.546	1.00
274_H	277_E	0.544	1.00
127_R	131_G	0.543	1.00
185_L	188_R	0.541	1.00
17_L	20_V	0.540	1.00
41_Q	44_E	0.537	1.00
26_E	31_I	0.537	1.00
37_R	41_Q	0.536	1.00
10_L	14_T	0.536	1.00
55_V	155_D	0.535	1.00
64_E	68_V	0.534	1.00
18_E	22_F	0.530	1.00
48_V	88_I	0.529	1.00
71_L	74_F	0.528	1.00
251_K	261_V	0.527	1.00
83_K	86_K	0.526	1.00
101_V	105_S	0.526	1.00
180_V	207_N	0.524	1.00
199_Y	298_D	0.523	1.00
27_N	33_V	0.521	1.00
104_V	107_I	0.519	1.00
97_I	149_I	0.516	1.00
56_E	128_H	0.516	1.00
133_I	138_L	0.515	1.00
91_E	142_L	0.514	1.00
29_F	33_V	0.513	1.00
60_K	123_Q	0.512	1.00
267_L	276_G	0.512	1.00
234_T	238_M	0.508	1.00
27_N	32_N	0.506	1.00
67_P	113_E	0.506	1.00
198_G	202_I	0.506	1.00
172_R	298_D	0.504	1.00
148_I	151_D	0.502	1.00
44_E	47_S	0.501	1.00
126_E	129_L	0.500	1.00
253_N	257_S	0.500	1.00
238_M	241_M	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3mm4A	1	0.4521	99.9	0.59	Contact Map
3breA	4	0.4125	99.9	0.627	Contact Map
1w25A	2	0.396	99.9	0.628	Contact Map
3r0jA	2	0.396	99.9	0.635	Contact Map
3dzdA	2	0.3861	99.9	0.636	Contact Map
4g97A	1	0.7393	99.9	0.636	Contact Map
3eq2A	2	0.3927	99.9	0.637	Contact Map
3n0rA	1	0.7756	99.9	0.641	Contact Map
3rqiA	2	0.3861	99.9	0.65	Contact Map
4l4uA	2	0.4125	99.9	0.651	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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