YABG - BACSU - GREMLIN Contacts

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YABG - Sporulation-specific protease YabG

UniProt:	P37548	Sequences: MQFQIGDMVARKSYQMDVLFRIIGIEQTSKGNSIAILHGDEVRLIADSDFSDLVAVKKDEQMMRKKKDESRMNESLELLRQDYKLLREKQEYYATSQYQHQEHYFHMPGKVLHLDGDEAYLKKCLNVYKKIGVPVYGIHCHEKKMSASIEVLLDKYRPDILVITGHDAYSKQKGGIDDLNAYRHSKHFVETVQTARKKIPHLDQLVIFAGACQSHFESLIRAGANFASSPSRVNIHALDPVYIVAKISFTPFMERINVWEVLRNTLTREKGLGGIETRGVLRIGMPYKSN	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	290 (280)
Sequences:	273 (209)
Seq/√Len:	12.5

BACSU - YABG

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
3_F	22_I	7.338	1.00
153_L	195_A	5.233	1.00
10_A	16_M	4.680	1.00
187_H	190_E	3.995	0.99
146_S	187_H	3.870	0.99
10_A	56_V	3.447	0.98
35_A	50_F	3.328	0.98
144_K	148_S	3.158	0.97
161_L	195_A	2.863	0.95
137_G	236_H	2.664	0.93
111_V	133_V	2.620	0.92
179_L	246_K	2.381	0.87
154_D	197_K	2.338	0.86
105_F	284_G	2.314	0.86
200_P	256_I	2.198	0.83
216_F	238_L	2.154	0.81
135_V	168_A	2.107	0.80
157_R	190_E	2.103	0.80
14_Y	171_K	2.093	0.79
237_A	276_E	2.066	0.79
121_L	137_G	2.002	0.76
3_F	161_L	2.001	0.76
22_I	243_I	1.990	0.76
45_I	149_I	1.947	0.74
22_I	71_R	1.944	0.74
79_L	262_L	1.935	0.74
189_V	218_S	1.913	0.73
194_T	197_K	1.850	0.70
247_I	261_V	1.844	0.70
178_D	181_A	1.833	0.69
163_I	191_T	1.818	0.69
44_L	276_E	1.769	0.67
121_L	236_H	1.762	0.66
241_V	266_L	1.677	0.62
251_P	261_V	1.656	0.61
108_P	125_L	1.648	0.61
250_T	260_E	1.644	0.61
146_S	190_E	1.632	0.60
23_I	124_C	1.610	0.59
16_M	54_V	1.599	0.58
161_L	244_V	1.590	0.58
21_R	181_A	1.571	0.57
137_G	267_T	1.567	0.57
42_V	176_I	1.558	0.56
111_V	134_P	1.557	0.56
96_S	260_E	1.555	0.56
15_Q	199_I	1.554	0.56
189_V	201_H	1.541	0.55
148_S	256_I	1.517	0.54
108_P	249_F	1.513	0.54
150_E	195_A	1.505	0.54
6_G	10_A	1.496	0.53
61_Q	248_S	1.491	0.53
123_K	145_M	1.490	0.53
108_P	203_D	1.468	0.52
133_V	245_A	1.440	0.50
90_Q	172_Q	1.438	0.50
83_Y	220_I	1.427	0.50
153_L	161_L	1.423	0.49
132_G	197_K	1.423	0.49
195_A	199_I	1.417	0.49
6_G	278_R	1.405	0.49
140_C	215_H	1.399	0.48
14_Y	110_K	1.398	0.48
245_A	287_Y	1.393	0.48
42_V	110_K	1.387	0.48
37_L	192_V	1.383	0.47
124_C	137_G	1.372	0.47
200_P	262_L	1.370	0.47
75_S	163_I	1.354	0.46
41_E	126_N	1.349	0.46
252_F	287_Y	1.345	0.45
146_S	149_I	1.339	0.45
76_L	249_F	1.331	0.45
22_I	34_I	1.326	0.45
185_S	281_L	1.326	0.45
63_M	110_K	1.315	0.44
21_R	242_Y	1.311	0.44
109_G	134_P	1.290	0.43
266_L	272_L	1.288	0.43
71_R	219_L	1.287	0.43
163_I	238_L	1.283	0.42
252_F	282_R	1.283	0.42
114_L	226_F	1.280	0.42
228_S	235_I	1.249	0.41
109_G	133_V	1.245	0.40
130_K	220_I	1.245	0.40
11_R	20_F	1.241	0.40
191_T	219_L	1.238	0.40
153_L	256_I	1.234	0.40
168_A	234_N	1.232	0.40
95_T	257_N	1.232	0.40
40_D	218_S	1.231	0.40
172_Q	233_V	1.226	0.40
133_V	248_S	1.223	0.39
27_Q	33_S	1.220	0.39
114_L	195_A	1.215	0.39
154_D	195_A	1.204	0.38
122_K	163_I	1.168	0.37
155_K	275_I	1.164	0.37
237_A	265_T	1.155	0.36
140_C	278_R	1.131	0.35
22_I	97_Q	1.126	0.35
178_D	267_T	1.124	0.35
6_G	180_N	1.120	0.34
282_R	286_P	1.114	0.34
82_D	174_G	1.100	0.34
104_Y	114_L	1.093	0.33
40_D	169_Y	1.090	0.33
10_A	18_V	1.086	0.33
185_S	218_S	1.086	0.33
11_R	52_D	1.082	0.33
145_M	242_Y	1.073	0.32
169_Y	234_N	1.072	0.32
38_H	287_Y	1.069	0.32
8_M	16_M	1.068	0.32
168_A	285_M	1.065	0.32
111_V	219_L	1.051	0.31
41_E	249_F	1.049	0.31
149_I	272_L	1.049	0.31
122_K	216_F	1.044	0.31
82_D	262_L	1.042	0.31
92_Y	98_Y	1.040	0.31
243_I	262_L	1.028	0.30
149_I	161_L	1.016	0.30
16_M	56_V	1.013	0.30
175_G	178_D	1.006	0.29
241_V	282_R	1.005	0.29
220_I	287_Y	1.004	0.29
155_K	248_S	1.001	0.29
129_K	153_L	0.997	0.29
25_I	63_M	0.995	0.29
35_A	44_L	0.990	0.29
204_Q	287_Y	0.982	0.28
91_E	94_A	0.978	0.28
162_V	229_S	0.975	0.28
50_F	272_L	0.973	0.28
119_A	272_L	0.971	0.28
62_M	179_L	0.971	0.28
27_Q	51_S	0.965	0.28
134_P	248_S	0.964	0.28
102_E	285_M	0.964	0.28
145_M	280_V	0.962	0.28
4_Q	55_A	0.958	0.27
44_L	193_Q	0.956	0.27
3_F	10_A	0.956	0.27
114_L	188_F	0.950	0.27
123_K	242_Y	0.947	0.27
202_L	258_V	0.947	0.27
6_G	83_Y	0.945	0.27
61_Q	256_I	0.943	0.27
138_I	191_T	0.942	0.27
151_V	267_T	0.940	0.27
125_L	155_K	0.937	0.27
105_F	282_R	0.934	0.26
55_A	138_I	0.933	0.26
213_Q	227_A	0.932	0.26
179_L	192_V	0.926	0.26
8_M	61_Q	0.925	0.26
87_R	90_Q	0.924	0.26
110_K	270_K	0.923	0.26
129_K	256_I	0.921	0.26
64_R	161_L	0.919	0.26
56_V	61_Q	0.918	0.26
144_K	147_A	0.917	0.26
24_G	186_K	0.915	0.26
88_E	93_Y	0.914	0.26
46_A	52_D	0.910	0.26
13_S	20_F	0.906	0.25
58_K	76_L	0.903	0.25
45_I	106_H	0.902	0.25
76_L	79_L	0.901	0.25
183_R	270_K	0.900	0.25
116_G	236_H	0.893	0.25
114_L	133_V	0.893	0.25
149_I	191_T	0.891	0.25
13_S	52_D	0.889	0.25
183_R	192_V	0.887	0.25
117_D	236_H	0.881	0.24
149_I	194_T	0.881	0.24
236_H	241_V	0.880	0.24
186_K	278_R	0.878	0.24
245_A	278_R	0.876	0.24
145_M	245_A	0.874	0.24
95_T	262_L	0.874	0.24
72_M	157_R	0.868	0.24
201_H	204_Q	0.863	0.24
262_L	285_M	0.863	0.24
242_Y	267_T	0.862	0.24
63_M	144_K	0.859	0.24
63_M	66_K	0.859	0.24
23_I	247_I	0.858	0.24
13_S	17_D	0.856	0.23
106_H	255_R	0.855	0.23
21_R	254_E	0.853	0.23
108_P	268_R	0.846	0.23
20_F	52_D	0.840	0.23
157_R	249_F	0.832	0.23
58_K	102_E	0.832	0.23
176_I	234_N	0.831	0.23
15_Q	198_K	0.831	0.23
195_A	237_A	0.829	0.22
130_K	259_W	0.828	0.22
11_R	17_D	0.828	0.22
109_G	248_S	0.828	0.22
203_D	273_G	0.827	0.22
200_P	260_E	0.823	0.22
241_V	277_T	0.822	0.22
4_Q	15_Q	0.821	0.22
51_S	201_H	0.820	0.22
40_D	263_R	0.819	0.22
236_H	267_T	0.819	0.22
215_H	285_M	0.819	0.22
18_V	136_Y	0.818	0.22
110_K	184_H	0.816	0.22
27_Q	66_K	0.815	0.22
195_A	276_E	0.814	0.22
108_P	278_R	0.811	0.22
191_T	251_P	0.811	0.22
123_K	127_V	0.810	0.22
79_L	245_A	0.800	0.21
19_L	83_Y	0.794	0.21
203_D	267_T	0.792	0.21
137_G	276_E	0.791	0.21
5_I	14_Y	0.791	0.21
8_M	244_V	0.791	0.21
90_Q	114_L	0.787	0.21
151_V	155_K	0.786	0.21
245_A	248_S	0.785	0.21
209_A	227_A	0.781	0.21
209_A	213_Q	0.781	0.21
203_D	278_R	0.774	0.21
50_F	174_G	0.773	0.21
105_F	286_P	0.772	0.21
69_E	72_M	0.768	0.20
135_V	234_N	0.765	0.20
200_P	254_E	0.763	0.20
4_Q	152_L	0.758	0.20
50_F	141_H	0.757	0.20
14_Y	149_I	0.756	0.20
156_Y	161_L	0.755	0.20
41_E	134_P	0.752	0.20
105_F	215_H	0.751	0.20
123_K	250_T	0.746	0.20
206_V	280_V	0.742	0.20
241_V	264_N	0.738	0.19
77_E	262_L	0.738	0.19
25_I	70_S	0.735	0.19
12_K	261_V	0.733	0.19
266_L	277_T	0.727	0.19
200_P	281_L	0.726	0.19
133_V	243_I	0.726	0.19
216_F	222_A	0.725	0.19
265_T	271_G	0.725	0.19
157_R	251_P	0.724	0.19
65_K	275_I	0.723	0.19
163_I	193_Q	0.722	0.19
105_F	216_F	0.722	0.19
74_E	175_G	0.720	0.19
171_K	222_A	0.719	0.19
140_C	148_S	0.719	0.19
153_L	206_V	0.718	0.19
11_R	46_A	0.717	0.19
207_I	278_R	0.716	0.19
236_H	265_T	0.714	0.19
89_K	219_L	0.712	0.19
24_G	51_S	0.710	0.19
84_K	262_L	0.709	0.18
203_D	282_R	0.709	0.18
170_S	181_A	0.709	0.18
90_Q	194_T	0.708	0.18
5_I	141_H	0.706	0.18
137_G	265_T	0.705	0.18
4_Q	190_E	0.705	0.18
225_N	277_T	0.704	0.18
126_N	133_V	0.701	0.18
114_L	149_I	0.700	0.18
121_L	276_E	0.697	0.18
109_G	120_Y	0.694	0.18
215_H	276_E	0.692	0.18
242_Y	280_V	0.691	0.18
3_F	183_R	0.691	0.18
208_F	241_V	0.690	0.18
54_V	101_Q	0.689	0.18
47_D	183_R	0.688	0.18
63_M	76_L	0.686	0.18
8_M	272_L	0.686	0.18
35_A	244_V	0.683	0.18
137_G	237_A	0.683	0.18
186_K	190_E	0.679	0.18
124_C	267_T	0.676	0.17
169_Y	244_V	0.675	0.17
81_Q	219_L	0.666	0.17
71_R	118_E	0.666	0.17
265_T	273_G	0.665	0.17
72_M	80_R	0.663	0.17
99_Q	118_E	0.661	0.17
157_R	255_R	0.660	0.17
248_S	255_R	0.658	0.17
91_E	95_T	0.656	0.17
14_Y	158_P	0.655	0.17
244_V	276_E	0.655	0.17
123_K	139_H	0.652	0.17
16_M	109_G	0.652	0.17
81_Q	174_G	0.651	0.17
34_I	63_M	0.649	0.17
99_Q	173_K	0.646	0.17
88_E	201_H	0.646	0.17
168_A	262_L	0.642	0.17
38_H	74_E	0.642	0.17
145_M	216_F	0.639	0.16
99_Q	119_A	0.638	0.16
113_H	284_G	0.637	0.16
109_G	125_L	0.635	0.16
35_A	41_E	0.634	0.16
180_N	233_V	0.632	0.16
129_K	150_E	0.631	0.16
139_H	215_H	0.629	0.16
51_S	96_S	0.629	0.16
148_S	186_K	0.626	0.16
143_K	243_I	0.625	0.16
57_K	104_Y	0.622	0.16
105_F	242_Y	0.622	0.16
17_D	52_D	0.621	0.16
84_K	99_Q	0.619	0.16
37_L	42_V	0.616	0.16
177_D	220_I	0.615	0.16
6_G	74_E	0.615	0.16
198_K	281_L	0.615	0.16
17_D	20_F	0.612	0.16
187_H	258_V	0.612	0.16
61_Q	88_E	0.612	0.16
39_G	43_R	0.608	0.16
140_C	254_E	0.608	0.16
249_F	282_R	0.607	0.16
57_K	190_E	0.604	0.15
36_I	219_L	0.602	0.15
135_V	269_E	0.599	0.15
16_M	248_S	0.598	0.15
134_P	187_H	0.598	0.15
96_S	114_L	0.598	0.15
54_V	68_D	0.597	0.15
236_H	276_E	0.597	0.15
45_I	114_L	0.596	0.15
8_M	191_T	0.596	0.15
78_L	145_M	0.596	0.15
19_L	60_E	0.594	0.15
72_M	79_L	0.590	0.15
55_A	59_D	0.587	0.15
8_M	50_F	0.586	0.15
270_K	287_Y	0.585	0.15
63_M	67_K	0.585	0.15
36_I	61_Q	0.579	0.15
79_L	263_R	0.578	0.15
44_L	106_H	0.577	0.15
170_S	173_K	0.576	0.15
4_Q	102_E	0.575	0.15
140_C	206_V	0.575	0.15
225_N	250_T	0.575	0.15
33_S	51_S	0.574	0.15
63_M	141_H	0.574	0.15
232_R	239_D	0.574	0.15
44_L	251_P	0.573	0.15
257_N	277_T	0.572	0.15
49_D	118_E	0.569	0.15
119_A	242_Y	0.569	0.15
34_I	71_R	0.568	0.15
202_L	246_K	0.568	0.15
22_I	84_K	0.565	0.14
78_L	264_N	0.565	0.14
132_G	285_M	0.561	0.14
66_K	71_R	0.560	0.14
90_Q	94_A	0.559	0.14
44_L	237_A	0.559	0.14
111_V	195_A	0.558	0.14
61_Q	261_V	0.556	0.14
236_H	282_R	0.555	0.14
40_D	70_S	0.552	0.14
44_L	255_R	0.549	0.14
105_F	220_I	0.549	0.14
96_S	100_H	0.547	0.14
203_D	265_T	0.547	0.14
82_D	264_N	0.545	0.14
216_F	247_I	0.543	0.14
108_P	155_K	0.541	0.14
185_S	189_V	0.539	0.14
62_M	243_I	0.539	0.14
149_I	243_I	0.539	0.14
4_Q	93_Y	0.537	0.14
22_I	143_K	0.530	0.14
34_I	66_K	0.528	0.14
57_K	87_R	0.527	0.14
54_V	58_K	0.526	0.13
140_C	178_D	0.525	0.13
33_S	37_L	0.525	0.13
116_G	120_Y	0.524	0.13
174_G	219_L	0.524	0.13
108_P	281_L	0.522	0.13
88_E	99_Q	0.521	0.13
71_R	188_F	0.518	0.13
216_F	234_N	0.517	0.13
40_D	157_R	0.516	0.13
189_V	287_Y	0.516	0.13
117_D	120_Y	0.514	0.13
34_I	141_H	0.514	0.13
79_L	193_Q	0.514	0.13
96_S	148_S	0.513	0.13
135_V	170_S	0.513	0.13
234_N	264_N	0.513	0.13
19_L	56_V	0.512	0.13
68_D	243_I	0.512	0.13
64_R	100_H	0.510	0.13
19_L	195_A	0.508	0.13
249_F	284_G	0.507	0.13
205_L	267_T	0.505	0.13
48_S	231_S	0.503	0.13
82_D	143_K	0.503	0.13
93_Y	118_E	0.502	0.13
38_H	218_S	0.500	0.13
58_K	219_L	0.500	0.13

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2plnA	2	0.3897	92.7	0.959	Contact Map
4r3uC	1	0.4276	87.7	0.963	Contact Map
3kyjB	1	0.4	86.9	0.964	Contact Map
4igaA	1	0.3931	84.6	0.965	Contact Map
3eulA	2	0.4207	84.3	0.965	Contact Map
4myrA	1	0.4207	79	0.967	Contact Map
2j48A	1	0.4034	78.9	0.967	Contact Map
4lzlA	1	0.4172	78.8	0.967	Contact Map
4jgiA	2	0.6276	78	0.967	Contact Map
1mvoA	2	0.3897	77.6	0.968	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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