YAAT - BACSU - GREMLIN Contacts

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YAAT - Stage 0 sporulation protein YaaT

UniProt:	P37541	Sequences: MYNVIGVRFKKAGKIYYFDPNGFHIEHDSCVIVETVRGVEYGQVVIANKQVDEHDVVLPLRKVIRVADDRDLLIVEENKQEALSAFDICQKKVIEHGLDMKLVDVEFTFDRNKVIFYFTADGRVDFRELVKDLASIFKTRIELRQIGVRDEAKMLGGIGPCGRMLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGLCGRLMCCLKYENDEYETAKEQLPDIGEMITTANGPAKVVGLNILERVLQVELINREKVIEYTWEELLEEGVVSAQTTD	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	275 (262)
Sequences:	1322 (776)
Seq/√Len:	48.0

BACSU - YAAT

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
96_H	132_D	5.385	1.00
163_R	169_T	4.090	1.00
228_T	231_G	4.007	1.00
41_Y	72_L	3.934	1.00
29_S	66_V	3.870	1.00
81_E	111_R	3.869	1.00
91_K	95_E	3.251	1.00
32_I	109_F	3.102	1.00
93_V	102_L	3.089	1.00
171_L	207_Y	2.795	1.00
3_N	48_N	2.769	1.00
227_T	232_P	2.668	1.00
218_Q	243_R	2.663	1.00
103_V	146_I	2.637	1.00
215_A	243_R	2.578	1.00
26_E	29_S	2.474	1.00
75_V	79_K	2.419	1.00
25_I	66_V	2.377	1.00
105_V	114_V	2.296	1.00
89_C	102_L	2.286	1.00
86_F	93_V	2.265	1.00
184_D	209_N	2.227	1.00
73_L	76_E	2.209	1.00
69_D	73_L	2.206	1.00
157_G	165_L	2.130	1.00
76_E	80_Q	2.065	1.00
23_F	66_V	2.042	1.00
19_D	62_K	2.016	0.99
30_C	43_Q	2.015	0.99
230_N	258_Y	1.985	0.99
39_V	64_I	1.982	0.99
6_G	15_I	1.902	0.99
32_I	41_Y	1.897	0.99
92_K	132_D	1.877	0.99
226_I	233_A	1.828	0.99
65_R	71_D	1.720	0.98
77_E	81_E	1.712	0.98
88_I	136_I	1.704	0.98
102_L	116_F	1.669	0.98
88_I	137_F	1.648	0.98
30_C	72_L	1.636	0.98
118_F	143_L	1.629	0.98
77_E	80_Q	1.620	0.98
120_A	124_V	1.604	0.97
88_I	92_K	1.571	0.97
95_E	136_I	1.570	0.97
87_D	91_K	1.542	0.97
7_V	31_V	1.521	0.96
219_L	238_L	1.519	0.96
233_A	249_L	1.512	0.96
30_C	41_Y	1.490	0.96
80_Q	84_S	1.453	0.95
211_E	214_T	1.440	0.95
221_D	224_E	1.433	0.95
89_C	93_V	1.432	0.95
25_I	44_V	1.431	0.95
232_P	250_I	1.431	0.95
3_N	50_Q	1.424	0.95
134_A	141_I	1.421	0.95
118_F	130_V	1.392	0.94
72_L	76_E	1.388	0.94
231_G	249_L	1.375	0.94
226_I	263_L	1.361	0.93
106_E	191_P	1.360	0.93
225_M	232_P	1.356	0.93
148_V	200_R	1.355	0.93
33_V	61_R	1.342	0.93
148_V	176_P	1.326	0.92
219_L	243_R	1.318	0.92
18_F	61_R	1.317	0.92
74_I	77_E	1.317	0.92
233_A	247_V	1.315	0.92
92_K	96_H	1.306	0.92
23_F	63_V	1.304	0.92
21_N	62_K	1.300	0.92
226_I	247_V	1.295	0.92
53_E	56_V	1.293	0.91
164_M	169_T	1.291	0.91
244_V	259_T	1.284	0.91
3_N	22_G	1.257	0.90
85_A	116_F	1.251	0.90
209_N	213_E	1.249	0.90
87_D	90_Q	1.249	0.90
259_T	262_E	1.248	0.90
165_L	197_L	1.244	0.90
187_L	190_N	1.233	0.89
41_Y	109_F	1.210	0.88
37_R	106_E	1.201	0.88
95_E	132_D	1.197	0.88
116_F	148_V	1.175	0.87
34_E	61_R	1.161	0.86
76_E	79_K	1.158	0.86
93_V	129_L	1.154	0.86
82_A	184_D	1.147	0.86
175_E	206_K	1.145	0.86
195_S	200_R	1.144	0.86
238_L	241_L	1.137	0.85
3_N	24_H	1.135	0.85
17_Y	143_L	1.132	0.85
103_V	119_T	1.132	0.85
5_I	48_N	1.118	0.84
191_P	194_I	1.113	0.84
99_D	120_A	1.112	0.84
96_H	128_E	1.106	0.84
9_F	16_Y	1.093	0.83
82_A	105_V	1.091	0.83
99_D	122_G	1.087	0.82
160_P	197_L	1.085	0.82
126_F	145_Q	1.072	0.82
70_R	74_I	1.071	0.81
25_I	31_V	1.070	0.81
185_Q	212_Y	1.069	0.81
157_G	183_K	1.066	0.81
31_V	63_V	1.062	0.81
234_K	248_E	1.058	0.81
6_G	46_I	1.057	0.81
121_D	124_V	1.049	0.80
165_L	169_T	1.043	0.80
120_A	132_D	1.043	0.80
42_G	61_R	1.031	0.79
16_Y	33_V	1.028	0.79
130_V	150_D	1.025	0.79
84_S	87_D	1.013	0.78
215_A	219_L	1.012	0.78
18_F	35_T	1.008	0.77
28_D	43_Q	1.001	0.77
209_N	216_K	0.982	0.75
14_K	121_D	0.981	0.75
228_T	249_L	0.980	0.75
32_I	71_D	0.978	0.75
118_F	183_K	0.975	0.75
237_G	242_E	0.972	0.75
228_T	233_A	0.969	0.75
81_E	107_F	0.963	0.74
151_E	176_P	0.962	0.74
79_K	82_A	0.962	0.74
239_N	242_E	0.960	0.74
113_K	119_T	0.956	0.74
41_Y	75_V	0.955	0.73
157_G	188_S	0.952	0.73
35_T	38_G	0.950	0.73
134_A	137_F	0.943	0.72
118_F	181_M	0.942	0.72
89_C	133_L	0.941	0.72
192_T	200_R	0.941	0.72
78_N	111_R	0.930	0.71
98_L	132_D	0.928	0.71
158_I	187_L	0.921	0.71
197_L	243_R	0.918	0.70
118_F	139_T	0.914	0.70
114_V	139_T	0.913	0.70
16_Y	35_T	0.911	0.70
100_M	126_F	0.899	0.69
118_F	150_D	0.898	0.69
105_V	143_L	0.889	0.68
62_K	124_V	0.889	0.68
219_L	260_W	0.888	0.68
16_Y	61_R	0.884	0.68
216_K	238_L	0.881	0.67
27_H	47_A	0.876	0.67
99_D	180_K	0.872	0.66
9_F	14_K	0.868	0.66
27_H	43_Q	0.864	0.66
85_A	118_F	0.863	0.66
245_L	263_L	0.862	0.66
111_R	114_V	0.858	0.65
234_K	243_R	0.854	0.65
239_N	244_V	0.849	0.64
237_G	246_Q	0.849	0.64
194_I	202_M	0.849	0.64
164_M	168_S	0.849	0.64
37_R	42_G	0.844	0.64
5_I	63_V	0.840	0.64
102_L	133_L	0.840	0.64
113_K	195_S	0.840	0.64
37_R	108_T	0.836	0.63
5_I	16_Y	0.835	0.63
34_E	64_I	0.834	0.63
152_A	172_G	0.826	0.62
197_L	211_E	0.825	0.62
96_H	143_L	0.824	0.62
258_Y	262_E	0.819	0.62
8_R	172_G	0.815	0.61
89_C	116_F	0.815	0.61
101_K	151_E	0.811	0.61
37_R	40_E	0.811	0.61
115_I	160_P	0.810	0.61
216_K	237_G	0.804	0.60
74_I	111_R	0.801	0.60
130_V	195_S	0.799	0.60
66_V	99_D	0.791	0.59
170_F	202_M	0.791	0.59
104_D	117_Y	0.791	0.59
218_Q	259_T	0.785	0.58
94_I	232_P	0.782	0.58
177_V	188_S	0.779	0.58
251_N	254_K	0.776	0.57
240_I	243_R	0.774	0.57
35_T	40_E	0.774	0.57
40_E	195_S	0.772	0.57
118_F	153_K	0.770	0.57
179_I	183_K	0.768	0.57
140_R	189_L	0.760	0.56
247_V	258_Y	0.748	0.55
32_I	65_R	0.747	0.55
57_V	96_H	0.745	0.54
183_K	189_L	0.743	0.54
114_V	143_L	0.742	0.54
225_M	234_K	0.742	0.54
236_V	246_Q	0.742	0.54
215_A	241_L	0.742	0.54
186_N	242_E	0.738	0.54
214_T	218_Q	0.737	0.54
105_V	243_R	0.734	0.53
92_K	95_E	0.734	0.53
218_Q	260_W	0.731	0.53
57_V	60_L	0.727	0.53
219_L	263_L	0.727	0.53
134_A	150_D	0.727	0.53
157_G	190_N	0.727	0.53
81_E	84_S	0.724	0.52
103_V	154_M	0.723	0.52
86_F	90_Q	0.720	0.52
92_K	136_I	0.717	0.52
99_D	118_F	0.717	0.52
194_I	200_R	0.715	0.51
219_L	241_L	0.713	0.51
86_F	105_V	0.712	0.51
230_N	256_I	0.710	0.51
117_Y	130_V	0.706	0.50
6_G	13_G	0.702	0.50
211_E	215_A	0.700	0.50
108_T	179_I	0.699	0.50
90_Q	93_V	0.696	0.49
244_V	257_E	0.693	0.49
14_K	61_R	0.692	0.49
151_E	195_S	0.692	0.49
151_E	155_L	0.692	0.49
56_V	59_P	0.691	0.49
90_Q	105_V	0.691	0.49
78_N	109_F	0.686	0.48
83_L	87_D	0.684	0.48
106_E	113_K	0.684	0.48
185_Q	208_E	0.684	0.48
84_S	136_I	0.684	0.48
255_V	258_Y	0.683	0.48
31_V	158_I	0.682	0.48
182_A	194_I	0.679	0.48
128_E	132_D	0.677	0.47
219_L	245_L	0.677	0.47
219_L	240_I	0.676	0.47
79_K	221_D	0.673	0.47
119_T	187_L	0.673	0.47
262_E	265_E	0.673	0.47
197_L	202_M	0.667	0.46
138_K	154_M	0.666	0.46
185_Q	201_L	0.666	0.46
10_K	16_Y	0.660	0.46
164_M	170_F	0.659	0.46
160_P	219_L	0.658	0.46
242_E	257_E	0.658	0.46
75_V	105_V	0.657	0.45
158_I	224_E	0.657	0.45
184_D	213_E	0.657	0.45
170_F	261_E	0.656	0.45
257_E	263_L	0.655	0.45
95_E	114_V	0.654	0.45
130_V	148_V	0.653	0.45
100_M	127_R	0.650	0.45
186_N	216_K	0.649	0.45
211_E	240_I	0.649	0.45
200_R	205_L	0.645	0.44
94_I	97_G	0.643	0.44
240_I	250_I	0.641	0.44
153_K	174_F	0.641	0.44
235_V	238_L	0.640	0.44
82_A	157_G	0.634	0.43
12_A	172_G	0.633	0.43
157_G	163_R	0.630	0.43
14_K	18_F	0.630	0.43
249_L	252_R	0.629	0.43
186_N	197_L	0.626	0.42
6_G	16_Y	0.626	0.42
31_V	42_G	0.625	0.42
70_R	73_L	0.625	0.42
257_E	262_E	0.624	0.42
183_K	194_I	0.621	0.42
18_F	109_F	0.621	0.42
247_V	263_L	0.620	0.42
255_V	260_W	0.619	0.42
30_C	75_V	0.618	0.42
92_K	130_V	0.615	0.41
168_S	236_V	0.613	0.41
85_A	107_F	0.612	0.41
93_V	133_L	0.610	0.41
30_C	58_L	0.609	0.41
49_K	52_D	0.608	0.41
90_Q	124_V	0.608	0.41
211_E	241_L	0.601	0.40
234_K	255_V	0.601	0.40
71_D	124_V	0.600	0.40
51_V	97_G	0.600	0.40
25_I	47_A	0.599	0.40
100_M	145_Q	0.597	0.40
52_D	55_D	0.596	0.40
3_N	21_N	0.594	0.39
192_T	213_E	0.592	0.39
165_L	168_S	0.592	0.39
163_R	188_S	0.592	0.39
87_D	131_K	0.591	0.39
106_E	117_Y	0.591	0.39
5_I	46_I	0.590	0.39
49_K	53_E	0.588	0.39
133_L	140_R	0.588	0.39
142_E	189_L	0.586	0.39
88_I	96_H	0.585	0.38
132_D	136_I	0.584	0.38
109_F	177_V	0.584	0.38
93_V	100_M	0.582	0.38
93_V	116_F	0.580	0.38
10_K	13_G	0.579	0.38
31_V	146_I	0.579	0.38
7_V	206_K	0.578	0.38
152_A	157_G	0.575	0.38
208_E	212_Y	0.575	0.38
177_V	210_D	0.575	0.38
5_I	115_I	0.574	0.37
239_N	256_I	0.574	0.37
83_L	124_V	0.573	0.37
60_L	63_V	0.573	0.37
49_K	79_K	0.572	0.37
176_P	180_K	0.571	0.37
118_F	247_V	0.571	0.37
69_D	72_L	0.570	0.37
5_I	25_I	0.567	0.37
161_C	204_C	0.567	0.37
153_K	226_I	0.566	0.37
224_E	237_G	0.565	0.37
82_A	160_P	0.565	0.37
6_G	171_L	0.564	0.36
20_P	51_V	0.562	0.36
174_F	179_I	0.560	0.36
15_I	46_I	0.557	0.36
94_I	222_I	0.555	0.36
69_D	210_D	0.553	0.35
40_E	108_T	0.553	0.35
15_I	18_F	0.552	0.35
40_E	88_I	0.551	0.35
243_R	260_W	0.548	0.35
18_F	63_V	0.547	0.35
160_P	172_G	0.546	0.35
101_K	107_F	0.544	0.35
30_C	74_I	0.536	0.34
182_A	205_L	0.534	0.34
24_H	64_I	0.533	0.34
10_K	207_Y	0.533	0.34
229_A	247_V	0.532	0.34
143_L	164_M	0.532	0.34
81_E	114_V	0.531	0.33
179_I	187_L	0.531	0.33
22_G	70_R	0.530	0.33
115_I	206_K	0.530	0.33
12_A	176_P	0.528	0.33
31_V	120_A	0.526	0.33
186_N	213_E	0.525	0.33
18_F	42_G	0.525	0.33
90_Q	112_N	0.524	0.33
85_A	148_V	0.523	0.33
126_F	131_K	0.523	0.33
4_V	60_L	0.522	0.33
178_S	181_M	0.521	0.33
116_F	134_A	0.521	0.33
158_I	165_L	0.520	0.32
218_Q	254_K	0.520	0.32
85_A	179_I	0.519	0.32
143_L	209_N	0.518	0.32
74_I	248_E	0.517	0.32
154_M	236_V	0.516	0.32
199_G	212_Y	0.515	0.32
52_D	262_E	0.515	0.32
210_D	221_D	0.513	0.32
140_R	144_R	0.513	0.32
24_H	48_N	0.510	0.32
43_Q	62_K	0.510	0.32
18_F	60_L	0.510	0.32
55_D	168_S	0.509	0.32
86_F	219_L	0.508	0.31
60_L	109_F	0.505	0.31
173_D	206_K	0.504	0.31
119_T	122_G	0.502	0.31
115_I	146_I	0.502	0.31
111_R	160_P	0.501	0.31
39_V	90_Q	0.500	0.31

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2qqrA	1	0.36	26.5	0.97	Contact Map
4hczA	1	0.1927	24.6	0.971	Contact Map
3j20N	1	0.2727	23.6	0.971	Contact Map
2lwjA	1	0.2291	19.8	0.972	Contact Map
2ldmA	1	0.2073	17.6	0.973	Contact Map
4qq6A	1	0.1891	17.2	0.973	Contact Map
4kbmB	1	0.2073	16.9	0.973	Contact Map
2xdpA	2	0.3745	16.3	0.973	Contact Map
2x8nA	1	0.2036	15.3	0.973	Contact Map
4a4fA	1	0.2109	14.6	0.974	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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