NOC - BACSU - GREMLIN Contacts

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NOC - Nucleoid occlusion protein

UniProt:	P37524	Sequences: MKHSFSRFFGLGEKEQEPEIAEHDTNKEEILEIPVNAIVPNRFQPRTIFSDEKIKELAMTIHTHGIIQPIVVRHTEEEGQYELIAGERRWRAVQSLEWEKIPAIIKDFSDTETASVALIENLQREELSSIEEAHAYARLLELHDLTQEALAQRLGKGQSTIANKLRLLKLPQPVQEAIMEKKITERHARALIPLKQPELQVTLLTEIIEKSLNVKQTEDRVVKMLEQGQRKPKPRRKAFSRDTRIAMNTIRQSLSMVEDSGVKLNTEEEEFEEYIQLTIRIPK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	283 (243)
Sequences:	5662 (4106)
Seq/√Len:	263.4

BACSU - NOC SP0J

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
32_E	100_K	4.220	1.00
39_V	80_Q	3.852	1.00
137_A	141_E	3.413	1.00
173_P	201_V	3.231	1.00
58_A	95_S	3.167	1.00
206_E	210_K	3.034	1.00
83_L	90_W	3.020	1.00
109_S	112_E	3.005	1.00
207_I	217_T	2.883	1.00
70_I	101_I	2.669	1.00
38_I	83_L	2.641	1.00
66_I	93_V	2.565	1.00
134_H	175_Q	2.502	1.00
37_A	80_Q	2.438	1.00
177_A	204_L	2.406	1.00
34_P	37_A	2.399	1.00
55_K	95_S	2.321	1.00
33_I	103_A	2.312	1.00
53_K	125_E	2.294	1.00
33_I	72_V	2.249	1.00
72_V	103_A	2.227	1.00
147_Q	162_A	2.119	1.00
157_G	160_T	2.109	1.00
55_K	59_M	2.084	1.00
176_E	180_E	1.999	1.00
54_I	58_A	1.995	1.00
73_R	82_E	1.980	1.00
212_L	220_R	1.965	1.00
138_R	142_L	1.941	1.00
66_I	102_P	1.937	1.00
51_D	95_S	1.935	1.00
54_I	95_S	1.926	1.00
90_W	94_Q	1.916	1.00
108_F	116_V	1.888	1.00
140_L	150_L	1.863	1.00
70_I	83_L	1.854	1.00
130_I	175_Q	1.850	1.00
149_A	152_Q	1.774	1.00
216_Q	219_D	1.724	1.00
51_D	55_K	1.706	1.00
93_V	98_W	1.705	1.00
59_M	62_H	1.695	1.00
38_I	72_V	1.677	1.00
39_V	82_E	1.675	1.00
35_V	90_W	1.673	1.00
73_R	110_D	1.665	1.00
85_A	120_E	1.626	1.00
70_I	103_A	1.613	1.00
134_H	179_M	1.613	1.00
187_H	214_V	1.585	1.00
45_P	117_A	1.570	1.00
199_L	202_T	1.552	1.00
39_V	75_T	1.544	1.00
203_L	220_R	1.526	1.00
41_N	113_T	1.520	1.00
171_P	200_Q	1.518	1.00
178_I	188_A	1.493	1.00
159_S	162_A	1.485	1.00
34_P	100_K	1.485	1.00
136_Y	154_L	1.481	1.00
75_T	82_E	1.444	1.00
67_I	71_V	1.440	1.00
50_S	53_K	1.424	1.00
93_V	96_L	1.413	1.00
132_E	164_K	1.408	1.00
206_E	220_R	1.406	1.00
171_P	201_V	1.385	1.00
132_E	156_K	1.367	1.00
205_T	209_E	1.363	1.00
177_A	208_I	1.361	1.00
61_I	66_I	1.354	1.00
55_K	58_A	1.346	1.00
87_E	91_R	1.338	1.00
148_E	152_Q	1.335	1.00
128_S	179_M	1.332	1.00
74_H	107_D	1.307	1.00
73_R	113_T	1.299	1.00
164_K	185_E	1.293	1.00
115_S	153_R	1.292	1.00
37_A	79_G	1.291	1.00
243_T	251_R	1.273	1.00
172_Q	175_Q	1.267	1.00
41_N	117_A	1.246	1.00
42_R	82_E	1.237	1.00
204_L	208_I	1.232	1.00
174_V	204_L	1.232	1.00
136_Y	150_L	1.223	1.00
201_V	205_T	1.214	1.00
66_I	101_I	1.205	1.00
31_L	105_I	1.203	1.00
61_I	93_V	1.201	1.00
64_H	112_E	1.183	1.00
35_V	93_V	1.182	1.00
40_P	90_W	1.181	1.00
58_A	96_L	1.173	1.00
75_T	78_E	1.162	1.00
53_K	56_E	1.158	1.00
249_T	253_S	1.149	1.00
58_A	62_H	1.145	1.00
150_L	161_I	1.134	1.00
32_E	102_P	1.122	1.00
190_A	217_T	1.115	1.00
47_T	134_H	1.103	1.00
176_E	179_M	1.092	1.00
64_H	115_S	1.082	1.00
75_T	80_Q	1.081	1.00
59_M	63_T	1.054	1.00
196_Q	199_L	1.043	1.00
136_Y	156_K	1.041	1.00
137_A	169_K	1.040	1.00
118_L	135_A	1.034	1.00
36_N	94_Q	1.033	1.00
84_I	108_F	1.025	1.00
74_H	79_G	1.020	1.00
217_T	220_R	1.018	1.00
60_T	119_I	1.014	1.00
160_T	164_K	1.011	1.00
140_L	165_L	1.005	1.00
207_I	212_L	1.003	1.00
93_V	101_I	1.001	1.00
49_F	87_E	0.987	1.00
173_P	205_T	0.984	1.00
194_L	203_L	0.982	1.00
212_L	217_T	0.982	1.00
177_A	182_K	0.977	1.00
66_I	70_I	0.975	1.00
49_F	91_R	0.974	1.00
41_N	82_E	0.971	1.00
187_H	190_A	0.970	1.00
163_N	189_R	0.967	1.00
114_A	138_R	0.965	1.00
140_L	147_Q	0.962	1.00
247_M	266_T	0.943	1.00
203_L	224_M	0.942	1.00
66_I	96_L	0.941	1.00
243_T	248_N	0.940	1.00
146_T	149_A	0.938	1.00
248_N	251_R	0.936	1.00
197_P	200_Q	0.936	1.00
35_V	94_Q	0.935	1.00
219_D	223_K	0.935	1.00
40_P	87_E	0.934	1.00
57_L	88_R	0.933	1.00
33_I	37_A	0.922	1.00
151_A	161_I	0.920	1.00
219_D	222_V	0.916	1.00
38_I	103_A	0.914	1.00
70_I	90_W	0.907	1.00
171_P	174_V	0.905	1.00
221_V	225_L	0.897	1.00
213_N	216_Q	0.890	1.00
217_T	221_V	0.880	1.00
156_K	160_T	0.867	1.00
175_Q	179_M	0.866	1.00
71_V	85_A	0.862	1.00
156_K	161_I	0.856	1.00
177_A	205_T	0.855	1.00
199_L	224_M	0.851	1.00
63_T	153_R	0.851	1.00
120_E	124_R	0.850	1.00
61_I	98_W	0.850	1.00
147_Q	158_Q	0.850	1.00
129_S	167_L	0.848	1.00
172_Q	176_E	0.841	0.99
203_L	217_T	0.840	0.99
248_N	252_Q	0.837	0.99
34_P	99_E	0.834	0.99
244_R	248_N	0.831	0.99
185_E	189_R	0.824	0.99
177_A	183_I	0.820	0.99
33_I	38_I	0.819	0.99
108_F	112_E	0.809	0.99
183_I	188_A	0.799	0.99
220_R	223_K	0.798	0.99
193_P	225_L	0.797	0.99
64_H	116_V	0.795	0.99
58_A	61_I	0.790	0.99
190_A	214_V	0.788	0.99
183_I	204_L	0.788	0.99
119_I	123_Q	0.781	0.99
69_P	104_I	0.777	0.99
246_A	249_T	0.775	0.99
243_T	247_M	0.771	0.99
71_V	104_I	0.771	0.99
245_I	249_T	0.770	0.99
67_I	116_V	0.765	0.99
88_R	125_E	0.757	0.99
63_T	152_Q	0.757	0.99
24_D	27_K	0.753	0.99
158_Q	162_A	0.747	0.99
184_T	187_H	0.746	0.99
38_I	101_I	0.742	0.99
197_P	201_V	0.735	0.99
252_Q	256_M	0.735	0.99
254_L	262_V	0.721	0.98
82_E	113_T	0.718	0.98
212_L	216_Q	0.718	0.98
174_V	200_Q	0.717	0.98
48_I	179_M	0.716	0.98
185_E	190_A	0.715	0.98
30_I	103_A	0.709	0.98
40_P	83_L	0.707	0.98
251_R	255_S	0.698	0.98
170_L	188_A	0.697	0.98
46_R	120_E	0.694	0.98
166_R	192_I	0.689	0.98
166_R	193_P	0.687	0.98
93_V	102_P	0.684	0.98
245_I	248_N	0.684	0.98
266_T	269_E	0.683	0.98
31_L	74_H	0.676	0.98
171_P	197_P	0.676	0.98
266_T	270_E	0.672	0.97
76_E	80_Q	0.670	0.97
75_T	79_G	0.667	0.97
143_H	150_L	0.666	0.97
187_H	191_L	0.663	0.97
52_E	55_K	0.661	0.97
83_L	87_E	0.661	0.97
189_R	192_I	0.659	0.97
214_V	217_T	0.656	0.97
195_K	225_L	0.655	0.97
261_G	265_N	0.654	0.97
220_R	224_M	0.652	0.97
62_H	96_L	0.650	0.97
118_L	139_L	0.649	0.97
111_T	115_S	0.646	0.97
145_L	153_R	0.646	0.97
194_L	200_Q	0.645	0.97
215_K	218_E	0.644	0.97
72_V	81_Y	0.643	0.97
160_T	185_E	0.642	0.97
108_F	113_T	0.641	0.97
62_H	97_E	0.638	0.97
151_A	158_Q	0.636	0.96
252_Q	260_S	0.635	0.96
202_T	206_E	0.635	0.96
36_N	99_E	0.635	0.96
40_P	91_R	0.634	0.96
118_L	122_L	0.633	0.96
62_H	98_W	0.633	0.96
54_I	61_I	0.632	0.96
246_A	250_I	0.631	0.96
85_A	116_V	0.631	0.96
178_I	183_I	0.630	0.96
195_K	199_L	0.630	0.96
57_L	92_A	0.629	0.96
177_A	180_E	0.628	0.96
81_Y	105_I	0.628	0.96
147_Q	151_A	0.627	0.96
54_I	96_L	0.627	0.96
136_Y	161_I	0.624	0.96
254_L	258_E	0.624	0.96
221_V	224_M	0.624	0.96
253_S	257_V	0.620	0.96
265_N	270_E	0.618	0.96
243_T	246_A	0.617	0.96
171_P	196_Q	0.616	0.96
49_F	88_R	0.616	0.96
140_L	143_H	0.615	0.96
43_F	110_D	0.615	0.96
38_I	90_W	0.615	0.96
136_Y	160_T	0.614	0.96
214_V	218_E	0.614	0.96
158_Q	186_R	0.612	0.96
267_E	270_E	0.612	0.96
133_A	168_L	0.609	0.95
163_N	166_R	0.606	0.95
111_T	142_L	0.603	0.95
58_A	92_A	0.600	0.95
200_Q	204_L	0.599	0.95
167_L	175_Q	0.598	0.95
243_T	263_K	0.597	0.95
166_R	189_R	0.597	0.95
32_E	103_A	0.596	0.95
148_E	158_Q	0.595	0.95
53_K	88_R	0.594	0.95
59_M	152_Q	0.589	0.95
189_R	193_P	0.588	0.95
121_N	124_R	0.587	0.95
119_I	139_L	0.586	0.94
35_V	101_I	0.585	0.94
87_E	90_W	0.584	0.94
31_L	81_Y	0.583	0.94
43_F	113_T	0.582	0.94
224_M	228_G	0.582	0.94
187_H	217_T	0.579	0.94
68_Q	120_E	0.578	0.94
186_R	190_A	0.578	0.94
140_L	162_A	0.577	0.94
56_E	59_M	0.577	0.94
169_K	195_K	0.576	0.94
210_K	216_Q	0.575	0.94
203_L	206_E	0.575	0.94
127_L	131_E	0.570	0.94
48_I	51_D	0.566	0.93
183_I	207_I	0.564	0.93
202_T	205_T	0.563	0.93
255_S	267_E	0.563	0.93
66_I	98_W	0.563	0.93
54_I	125_E	0.560	0.93
252_Q	255_S	0.559	0.93
263_K	266_T	0.559	0.93
186_R	215_K	0.556	0.93
67_I	119_I	0.556	0.93
66_I	116_V	0.556	0.93
258_E	264_L	0.553	0.93
226_E	231_K	0.553	0.93
213_N	217_T	0.550	0.92
255_S	259_D	0.550	0.92
223_K	226_E	0.550	0.92
229_Q	232_P	0.549	0.92
28_E	106_K	0.549	0.92
73_R	84_I	0.547	0.92
71_V	108_F	0.546	0.92
244_R	266_T	0.545	0.92
242_D	245_I	0.545	0.92
44_Q	49_F	0.543	0.92
167_L	185_E	0.542	0.92
44_Q	117_A	0.542	0.92
86_G	89_R	0.541	0.92
151_A	157_G	0.541	0.92
136_Y	164_K	0.541	0.92
145_L	149_A	0.539	0.92
44_Q	87_E	0.538	0.92
168_L	213_N	0.538	0.92
71_V	116_V	0.538	0.92
132_E	136_Y	0.537	0.91
173_P	200_Q	0.537	0.91
130_I	168_L	0.536	0.91
46_R	117_A	0.535	0.91
67_I	106_K	0.535	0.91
192_I	200_Q	0.534	0.91
114_A	143_H	0.533	0.91
122_L	135_A	0.533	0.91
128_S	131_E	0.532	0.91
130_I	134_H	0.532	0.91
43_F	82_E	0.529	0.91
112_E	116_V	0.526	0.91
186_R	189_R	0.526	0.91
47_T	131_E	0.525	0.91
225_L	228_G	0.523	0.90
147_Q	150_L	0.520	0.90
228_G	232_P	0.519	0.90
23_H	26_N	0.518	0.90
230_R	240_S	0.516	0.90
139_L	150_L	0.515	0.90
54_I	57_L	0.514	0.90
49_F	54_I	0.513	0.90
150_L	160_T	0.513	0.90
251_R	259_D	0.511	0.89
249_T	256_M	0.510	0.89
248_N	256_M	0.510	0.89
222_V	225_L	0.510	0.89
248_N	253_S	0.509	0.89
182_K	208_I	0.509	0.89
244_R	249_T	0.508	0.89
246_A	260_S	0.508	0.89
132_E	160_T	0.505	0.89
242_D	248_N	0.504	0.89
259_D	264_L	0.501	0.88
39_V	42_R	0.501	0.88
45_P	118_L	0.501	0.88
92_A	121_N	0.500	0.88

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4umkA	2	0.682	100	0.265	Contact Map
1vz0A	3	0.6784	100	0.277	Contact Map
1r71A	2	0.3922	99.9	0.589	Contact Map
1vk1A	1	0.6396	99.9	0.632	Contact Map
1yzsA	1	0.4064	99.8	0.633	Contact Map
1xw3A	1	0.3675	99.8	0.634	Contact Map
3mkyB	2	0.3852	99.6	0.712	Contact Map
1zx4A	2	0.5512	98.2	0.839	Contact Map
2hwjA	5	0.6537	97.4	0.864	Contact Map
3vwbA	1	0.4064	93.4	0.901	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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