GREMLIN Database
PPAC - Manganese-dependent inorganic pyrophosphatase
UniProt: P37487 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 309 (303)
Sequences: 1676 (1134)
Seq/√Len: 65.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
144_S180_Y4.7921.00
70_G90_Q4.2931.00
204_A215_E3.9281.00
216_I300_V3.7611.00
128_K168_E3.7571.00
176_D179_E3.6181.00
135_E138_I3.4741.00
160_D164_A3.2981.00
253_V262_S3.2911.00
143_L175_V3.2521.00
10_Q84_K2.9651.00
160_D163_V2.8971.00
196_V233_E2.8491.00
125_K161_Q2.8421.00
112_R129_E2.8091.00
256_D259_E2.6551.00
47_Q51_D2.6291.00
72_I93_E2.5961.00
163_V167_K2.5521.00
289_L292_V2.4281.00
152_L181_G2.3661.00
179_E183_N2.3291.00
195_T198_E2.2271.00
143_L166_A2.2241.00
27_K93_E2.2171.00
280_V287_A2.2171.00
239_S243_A2.1881.00
114_E125_K2.1791.00
275_E281_T2.1711.00
124_N165_A2.1611.00
120_A142_M2.1591.00
63_T66_N2.1351.00
24_A34_A2.1281.00
117_G150_S2.0631.00
153_F166_A2.0371.00
147_I152_L2.0151.00
38_R60_L1.9961.00
231_Q235_E1.9671.00
253_V299_V1.9551.00
197_E244_E1.9461.00
209_L214_V1.9301.00
61_V64_A1.9241.00
153_F158_C1.8961.00
90_Q107_E1.8451.00
124_N168_E1.8011.00
166_A177_A1.7881.00
35_E59_R1.7561.00
167_K171_E1.7521.00
26_L30_L1.7461.00
240_K244_E1.7421.00
55_Q141_L1.7230.99
253_V264_A1.7200.99
242_V267_I1.6960.99
23_Y93_E1.6880.99
116_V121_T1.6440.99
170_A177_A1.6410.99
230_R233_E1.6220.99
236_A240_K1.6160.99
237_V240_K1.6010.99
238_I250_F1.5800.99
25_D135_E1.5770.99
25_D127_Y1.5620.99
63_T85_D1.5600.99
71_V86_I1.5430.99
19_S120_A1.5360.99
23_Y27_K1.5330.99
206_E213_K1.5210.99
218_Q255_T1.5140.99
53_F140_G1.5060.99
263_L286_T1.4640.98
30_L130_N1.4610.98
23_Y72_I1.4450.98
24_A28_N1.4410.98
14_T49_A1.4340.98
206_E245_K1.4230.98
51_D54_K1.4140.98
100_I111_Y1.4060.98
114_E161_Q1.3910.98
212_K248_D1.3900.98
75_D97_H1.3880.98
118_C142_M1.3780.97
136_K174_G1.3740.97
25_D29_K1.3680.97
215_E247_L1.3600.97
52_Y183_N1.3570.97
124_N128_K1.3560.97
151_L293_V1.3560.97
261_D288_L1.3400.97
151_L181_G1.3190.97
112_R126_M1.3180.97
55_Q137_E1.3180.97
82_S86_I1.3160.97
196_V237_V1.3140.97
118_C145_A1.3000.96
21_I50_L1.2930.96
165_A168_E1.2810.96
200_I237_V1.2620.96
22_A25_D1.2450.95
197_E237_V1.2450.95
79_R91_V1.2420.95
231_Q286_T1.2390.95
76_H82_S1.2290.95
107_E110_Y1.2290.95
27_K72_I1.2260.95
208_T211_S1.2190.95
22_A120_A1.2180.95
215_E250_F1.2110.95
28_N33_N1.2050.95
147_I166_A1.1910.94
79_R87_E1.1900.94
152_L178_E1.1710.94
77_N98_H1.1650.93
239_S242_V1.1460.93
18_C22_A1.1370.93
37_V61_V1.1310.92
188_G220_N1.1220.92
22_A138_I1.1100.92
181_G185_L1.1070.92
209_L300_V1.1050.92
42_V60_L1.1040.91
94_V103_F1.1040.91
18_C141_L1.0870.91
200_I238_I1.0800.91
221_T254_I1.0780.90
120_A124_N1.0720.90
25_D138_I1.0700.90
23_Y95_I1.0700.90
42_V47_Q1.0570.90
6_I25_D1.0550.90
303_L307_M1.0550.90
204_A217_A1.0520.89
145_A153_F1.0390.89
158_C163_V1.0290.88
240_K243_A1.0230.88
124_N142_M1.0210.88
76_H83_I1.0150.88
78_E99_R1.0100.87
95_I126_M0.9960.87
238_I267_I0.9950.87
17_I38_R0.9930.86
9_H12_P0.9740.85
64_A83_I0.9630.85
5_L35_E0.9590.84
10_Q76_H0.9580.84
207_F300_V0.9540.84
27_K30_L0.9540.84
28_N34_A0.9530.84
196_V230_R0.9530.84
38_R42_V0.9460.84
121_T165_A0.9410.83
26_L127_Y0.9350.83
138_I142_M0.9270.82
235_E285_N0.9270.82
7_F71_V0.9220.82
238_I252_L0.9220.82
118_C121_T0.9210.82
70_G92_L0.9210.82
194_K202_L0.9200.82
134_I142_M0.9170.82
227_V234_L0.9140.82
8_G13_D0.9090.81
65_A88_E0.9090.81
18_C49_A0.9030.81
300_V303_L0.9030.81
68_V89_V0.8970.80
206_E247_L0.8900.80
100_I113_A0.8900.80
97_H149_D0.8900.80
155_S298_Q0.8870.80
251_L303_L0.8830.79
7_F16_T0.8830.79
150_S153_F0.8770.79
168_E171_E0.8760.79
171_E174_G0.8750.79
6_I24_A0.8700.78
49_A184_M0.8700.78
224_I228_K0.8680.78
217_A252_L0.8670.78
75_D98_H0.8650.78
49_A150_S0.8620.78
118_C146_I0.8520.77
5_L37_V0.8500.77
118_C153_F0.8460.76
260_N293_V0.8440.76
216_I303_L0.8350.75
154_K260_N0.8340.75
97_H117_G0.8330.75
16_T120_A0.8310.75
208_T213_K0.8310.75
251_L264_A0.8300.75
183_N240_K0.8220.74
143_L173_A0.8220.74
131_N134_I0.8210.74
166_A170_A0.8190.74
65_A85_D0.8190.74
186_K223_D0.8180.74
239_S267_I0.8120.73
25_D28_N0.8100.73
201_S241_V0.8090.73
241_V244_E0.8070.73
75_D96_D0.8040.73
152_L177_A0.8040.73
161_Q164_A0.8020.72
300_V304_T0.8000.72
278_F306_A0.8000.72
7_F39_L0.7980.72
8_G16_T0.7970.72
232_A236_A0.7950.72
94_V111_Y0.7940.72
8_G15_D0.7900.71
191_L221_T0.7860.71
264_A303_L0.7810.70
225_E228_K0.7780.70
7_F37_V0.7750.70
6_I34_A0.7750.70
125_K165_A0.7720.69
212_K270_E0.7700.69
6_I72_I0.7670.69
119_T123_L0.7630.69
105_T108_P0.7620.68
241_V250_F0.7620.68
252_L257_I0.7610.68
127_Y138_I0.7600.68
79_R86_I0.7600.68
112_R125_K0.7580.68
75_D149_D0.7530.67
37_V59_R0.7470.67
77_N111_Y0.7470.67
146_I165_A0.7430.66
170_A173_A0.7420.66
21_I24_A0.7410.66
22_A142_M0.7400.66
224_I256_D0.7390.66
23_Y127_Y0.7390.66
39_L201_S0.7390.66
209_L304_T0.7360.66
19_S119_T0.7350.66
17_I46_T0.7330.65
126_M129_E0.7320.65
199_L217_A0.7310.65
194_K198_E0.7290.65
274_V277_A0.7280.65
146_I169_L0.7270.65
38_R41_Q0.7260.65
204_A247_L0.7250.65
22_A123_L0.7240.64
190_D193_K0.7240.64
9_H43_N0.7210.64
44_G190_D0.7170.64
124_N134_I0.7170.64
31_G34_A0.7150.63
99_R304_T0.7130.63
146_I153_F0.7110.63
120_A145_A0.7110.63
21_I55_Q0.7110.63
228_K263_L0.7110.63
72_I92_L0.7110.63
216_I253_V0.7090.63
220_N289_L0.7090.63
261_D290_K0.7080.63
23_Y123_L0.7080.63
75_D117_G0.7070.63
193_K230_R0.7070.63
233_E236_A0.7060.62
274_V287_A0.7010.62
224_I254_I0.6990.62
18_C145_A0.6950.61
272_A285_N0.6950.61
282_L287_A0.6940.61
234_L265_L0.6930.61
20_A74_V0.6930.61
224_I263_L0.6920.61
221_T224_I0.6920.61
150_S158_C0.6910.61
266_A282_L0.6870.60
171_E178_E0.6850.60
69_N89_V0.6850.60
221_T256_D0.6820.60
29_K135_E0.6810.60
149_D162_D0.6760.59
16_T93_E0.6750.59
190_D247_L0.6740.59
181_G257_I0.6740.59
204_A250_F0.6720.59
156_P298_Q0.6720.59
158_C166_A0.6700.58
167_K177_A0.6700.58
73_L76_H0.6660.58
128_K164_A0.6600.57
273_K276_K0.6590.57
164_A167_K0.6580.57
9_H157_T0.6570.57
193_K237_V0.6560.57
47_Q57_S0.6550.57
95_I102_N0.6540.57
272_A275_E0.6540.57
24_A142_M0.6470.56
241_V247_L0.6470.56
248_D270_E0.6470.56
54_K137_E0.6460.56
234_L252_L0.6460.56
208_T212_K0.6440.55
134_I168_E0.6430.55
18_C38_R0.6400.55
78_E81_Q0.6390.55
77_N99_R0.6370.55
217_A250_F0.6360.55
17_I50_L0.6350.54
218_Q295_R0.6350.54
74_V123_L0.6350.54
120_A192_S0.6340.54
218_Q262_S0.6290.54
263_L288_L0.6280.54
28_N32_F0.6280.54
197_E241_V0.6270.54
42_V57_S0.6270.54
18_C27_K0.6270.54
247_L299_V0.6270.54
137_E141_L0.6260.53
41_Q47_Q0.6250.53
191_L199_L0.6250.53
182_L186_K0.6240.53
56_E59_R0.6240.53
19_S23_Y0.6230.53
199_L258_L0.6230.53
21_I138_I0.6210.53
118_C165_A0.6200.53
139_A169_L0.6200.53
291_G294_S0.6190.53
48_Y52_Y0.6180.52
255_T294_S0.6130.52
252_L265_L0.6110.52
235_E239_S0.6090.51
121_T159_T0.6080.51
151_L188_G0.6070.51
154_K178_E0.6070.51
214_V300_V0.6050.51
95_I123_L0.6050.51
232_A235_E0.6030.51
197_E236_A0.6030.51
235_E286_T0.6020.51
9_H296_K0.6000.50
80_Q102_N0.5990.50
213_K247_L0.5980.50
138_I141_L0.5960.50
202_L217_A0.5940.50
160_D243_A0.5930.50
144_S159_T0.5930.50
270_E273_K0.5910.49
110_Y114_E0.5900.49
9_H15_D0.5900.49
27_K34_A0.5890.49
13_D16_T0.5850.49
174_G177_A0.5850.49
244_E272_A0.5830.48
161_Q165_A0.5810.48
256_D261_D0.5800.48
234_L242_V0.5790.48
96_D162_D0.5780.48
117_G139_A0.5780.48
67_E272_A0.5780.48
242_V247_L0.5770.48
118_C144_S0.5770.48
139_A172_I0.5760.48
44_G193_K0.5750.47
266_A274_V0.5730.47
273_K307_M0.5730.47
171_E229_K0.5680.47
189_A226_D0.5670.47
154_K222_V0.5670.47
264_A274_V0.5670.47
185_L189_A0.5660.46
228_K254_I0.5610.46
116_V161_Q0.5590.46
262_S293_V0.5580.45
120_A123_L0.5570.45
238_I271_A0.5550.45
184_M200_I0.5550.45
120_A130_N0.5550.45
202_L214_V0.5530.45
16_T95_I0.5510.45
47_Q54_K0.5510.45
108_P157_T0.5460.44
109_L113_A0.5460.44
235_E284_N0.5450.44
142_M145_A0.5450.44
20_A49_A0.5450.44
65_A69_N0.5440.44
99_R115_P0.5440.44
160_D232_A0.5430.44
152_L258_L0.5430.44
52_Y55_Q0.5410.43
149_D203_D0.5410.43
143_L169_L0.5390.43
160_D167_K0.5390.43
11_N17_I0.5380.43
154_K163_V0.5360.43
164_A168_E0.5360.43
199_L202_L0.5350.43
56_E86_I0.5350.43
79_R303_L0.5330.43
33_N69_N0.5320.42
154_K181_G0.5310.42
222_V242_V0.5310.42
94_V234_L0.5300.42
197_E201_S0.5280.42
71_V285_N0.5270.42
224_I237_V0.5270.42
278_F302_V0.5240.42
199_L227_V0.5240.42
253_V263_L0.5230.41
122_I170_A0.5220.41
157_T294_S0.5210.41
235_E238_I0.5210.41
221_T226_D0.5200.41
9_H14_T0.5190.41
193_K252_L0.5190.41
238_I306_A0.5180.41
248_D272_A0.5180.41
49_A137_E0.5180.41
19_S46_T0.5180.41
29_K33_N0.5170.41
97_H297_K0.5170.41
226_D229_K0.5160.41
75_D301_P0.5160.41
118_C303_L0.5150.41
192_S195_T0.5150.41
157_T296_K0.5150.41
86_I89_V0.5140.40
295_R298_Q0.5130.40
179_E307_M0.5130.40
51_D56_E0.5120.40
31_G58_P0.5120.40
77_N102_N0.5110.40
267_I270_E0.5100.40
97_H301_P0.5080.40
117_G149_D0.5080.40
186_K274_V0.5070.40
208_T229_K0.5060.40
201_S237_V0.5060.40
96_D301_P0.5060.40
206_E230_R0.5060.40
167_K246_N0.5050.39
262_S289_L0.5030.39
108_P296_K0.5030.39
242_V269_N0.5030.39
152_L223_D0.5020.39
223_D238_I0.5020.39
36_P40_G0.5020.39
21_I140_G0.5020.39
64_A70_G0.5020.39
213_K248_D0.5010.39
105_T294_S0.5010.39
228_K233_E0.5000.39
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1k20A 2 0.9968 100 0.313 Contact Map
2qb7A 1 0.9871 100 0.332 Contact Map
2hawA 2 0.9968 100 0.343 Contact Map
2eb0A 2 0.9644 100 0.345 Contact Map
4rpaA 3 0.9935 100 0.351 Contact Map
1wpnA 2 0.6052 100 0.593 Contact Map
4py9A 2 0.9547 100 0.655 Contact Map
4ls9A 2 0.9288 100 0.679 Contact Map
3devA 2 0.9256 100 0.681 Contact Map
2zxrA 1 0.7767 99.1 0.856 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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