GREMLIN Database
SP2E - Stage II sporulation protein E
UniProt: P37475 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 827 (768)
Sequences: 392 (336)
Seq/√Len: 12.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
544_F573_H12.2201.00
544_F581_S10.0551.00
573_H581_S9.0701.00
624_A802_V5.1421.00
544_F550_S5.1411.00
740_L777_I4.8521.00
243_G268_G3.8490.99
402_C408_C3.7100.99
624_A800_V3.6420.99
677_Y713_V3.4790.98
268_G272_G3.3840.98
386_E804_I3.2780.98
680_L745_S3.1870.97
558_L585_F3.1810.97
402_C446_C3.0840.96
328_V471_K3.0270.96
546_G573_H2.9850.96
243_G272_G2.9620.95
546_G550_S2.8880.95
742_I766_M2.8620.94
399_C402_C2.7990.94
591_Y703_F2.7810.94
408_C446_C2.7700.93
399_C446_C2.7670.93
633_G654_G2.7350.93
363_V366_A2.6880.92
550_S573_H2.5860.91
184_K781_L2.5420.90
664_I692_C2.5360.90
399_C408_C2.5080.89
571_E585_F2.4900.89
627_S678_S2.4890.89
442_F452_V2.4650.88
691_S732_S2.4280.88
46_Y356_K2.4220.88
409_W416_T2.4170.87
778_A801_V2.3840.87
220_H309_E2.3300.85
50_G800_V2.3250.85
558_L741_L2.3190.85
217_Q309_E2.2630.84
309_E761_W2.2560.83
173_F472_K2.2440.83
55_R207_G2.2200.82
79_D194_I2.2120.82
758_H762_M2.1890.82
44_F222_L2.1780.81
239_G243_G2.1590.81
442_F664_I2.0920.78
239_G272_G2.0920.78
466_H469_L2.0690.78
218_A279_K2.0560.77
694_F804_I2.0290.76
287_I710_V2.0150.76
550_S581_S2.0080.75
227_V646_L2.0040.75
721_G746_D1.9990.75
721_G747_G1.9990.75
221_I226_V1.9670.74
101_L177_L1.9650.74
265_A292_L1.9600.74
746_D795_D1.9500.73
746_D796_D1.9500.73
747_G795_D1.9500.73
747_G796_D1.9500.73
644_I663_T1.9470.73
35_L781_L1.9300.72
196_C282_A1.9230.72
593_V685_I1.9130.72
172_I208_L1.9030.71
356_K561_I1.8930.71
771_T779_D1.8890.71
108_L117_A1.8870.71
483_Q743_M1.8810.70
66_A103_I1.8750.70
750_E758_H1.8690.70
207_G417_Y1.8680.70
782_M799_V1.8620.69
223_A410_V1.8550.69
363_V585_F1.8490.69
221_I312_I1.8410.69
681_D696_K1.8260.68
190_K781_L1.8260.68
279_K805_D1.8120.67
643_T714_Q1.8110.67
108_L526_V1.7890.66
137_A163_A1.7800.66
289_G608_S1.7660.65
284_I656_D1.7650.65
91_G556_P1.7640.65
364_F367_L1.7550.65
596_G778_A1.7530.65
750_E782_M1.7530.65
146_A279_K1.7520.65
698_G721_G1.7430.64
722_I775_Q1.7390.64
704_I713_V1.7310.64
239_G475_D1.7270.63
58_I785_V1.7250.63
493_D526_V1.7230.63
610_D628_D1.7220.63
610_D631_G1.7220.63
628_D631_G1.7220.63
107_L167_F1.7200.63
240_C243_G1.7080.63
456_I649_K1.7060.62
721_G795_D1.7020.62
721_G796_D1.7020.62
84_A295_L1.6970.62
285_G289_G1.6900.62
172_I176_S1.6870.62
163_A195_I1.6700.61
180_F767_K1.6670.61
719_P739_D1.6580.60
192_E296_Y1.6510.60
484_L608_S1.6510.60
594_S648_E1.6360.59
48_V119_F1.6280.59
323_S685_I1.6250.59
266_F520_G1.6230.58
70_F500_R1.6210.58
143_F692_C1.6140.58
629_G696_K1.6130.58
632_N635_R1.6100.58
703_F712_K1.5860.57
196_C289_G1.5840.56
629_G636_A1.5810.56
357_V690_A1.5810.56
47_V87_A1.5740.56
128_L204_V1.5700.56
715_A728_V1.5680.56
221_I800_V1.5610.55
399_C629_G1.5580.55
137_A440_K1.5490.55
130_I799_V1.5490.55
423_V442_F1.5480.55
488_S743_M1.5230.53
265_A706_R1.5150.53
367_L742_I1.5150.53
446_C629_G1.5140.53
50_G712_K1.5040.52
348_K509_E1.5000.52
202_A743_M1.4980.52
279_K724_N1.4980.52
702_S720_I1.4910.52
593_V748_I1.4900.52
78_R566_I1.4880.52
288_V500_R1.4800.51
432_Y457_E1.4780.51
281_G285_G1.4640.50
309_E701_P1.4630.50
722_I726_F1.4580.50
50_G69_F1.4500.50
498_I561_I1.4500.50
287_I762_M1.4450.50
559_S608_S1.4400.49
609_G628_D1.4390.49
609_G631_G1.4390.49
48_V591_Y1.4350.49
745_S805_D1.4330.49
108_L146_A1.4260.49
312_I336_V1.4220.48
279_K438_L1.4160.48
240_C272_G1.4150.48
56_A199_I1.4020.47
706_R739_D1.3950.47
215_G288_V1.3930.47
83_Y563_E1.3870.47
216_M221_I1.3770.46
66_A457_E1.3730.46
629_G681_D1.3660.46
667_I671_R1.3660.46
194_I237_S1.3650.45
706_R736_K1.3650.45
103_I448_K1.3620.45
613_S648_E1.3620.45
93_L100_S1.3580.45
45_I777_I1.3560.45
610_D629_G1.3490.45
109_A213_Y1.3470.45
427_T460_L1.3470.45
522_E804_I1.3430.44
230_F262_S1.3410.44
397_H674_D1.3390.44
356_K583_V1.3390.44
433_A453_E1.3360.44
356_K597_A1.3350.44
274_L723_I1.3340.44
748_I782_M1.3320.44
232_F317_F1.3310.44
159_A283_A1.3300.44
364_F371_F1.3300.44
689_D748_I1.3210.43
696_K746_D1.3180.43
696_K747_G1.3180.43
581_S620_R1.3170.43
694_F797_M1.3140.43
626_I804_I1.3130.43
635_R668_L1.3090.43
279_K646_L1.3040.42
643_T668_L1.3030.42
83_Y803_R1.2970.42
322_Q759_D1.2940.42
45_I107_L1.2900.42
387_V463_H1.2890.42
489_E512_I1.2880.42
34_H685_I1.2870.42
497_E735_L1.2850.42
232_F582_H1.2780.41
76_I725_E1.2780.41
68_P494_F1.2740.41
554_I608_S1.2740.41
564_E587_S1.2720.41
48_V713_V1.2700.41
47_V237_S1.2650.41
610_D746_D1.2600.40
610_D747_G1.2600.40
628_D746_D1.2600.40
628_D747_G1.2600.40
631_G746_D1.2600.40
631_G747_G1.2600.40
528_I566_I1.2550.40
296_Y639_E1.2550.40
609_G679_T1.2520.40
636_A696_K1.2510.40
492_A753_K1.2480.40
55_R247_G1.2470.40
145_Y167_F1.2440.40
56_A332_I1.2430.39
157_V301_A1.2380.39
414_D469_L1.2350.39
367_L370_S1.2290.39
696_K719_P1.2240.39
220_H261_M1.2210.38
128_L661_I1.2200.38
132_V166_S1.2190.38
91_G409_W1.2170.38
399_C696_K1.2140.38
785_V799_V1.2130.38
357_V370_S1.2120.38
113_F213_Y1.2120.38
240_C397_H1.2110.38
240_C268_G1.2100.38
524_Q541_T1.2080.38
399_C610_D1.2060.38
399_C628_D1.2060.38
399_C631_G1.2060.38
48_V704_I1.2050.38
270_L475_D1.1960.37
399_C636_A1.1960.37
546_G581_S1.1930.37
231_S269_L1.1910.37
222_L315_C1.1890.37
356_K477_R1.1880.37
68_P439_K1.1840.37
446_C696_K1.1830.37
719_P746_D1.1800.36
719_P747_G1.1800.36
156_Y213_Y1.1750.36
653_S743_M1.1720.36
82_F87_A1.1690.36
55_R409_W1.1600.36
249_I785_V1.1550.35
393_K453_E1.1520.35
269_L745_S1.1490.35
402_C629_G1.1480.35
201_I218_A1.1460.35
205_L226_V1.1450.35
264_L512_I1.1440.35
683_S741_L1.1410.35
521_I672_T1.1410.35
446_C636_A1.1350.34
177_L703_F1.1350.34
408_C629_G1.1330.34
596_G604_G1.1330.34
680_L702_S1.1330.34
367_L371_F1.1300.34
633_G636_A1.1250.34
121_T361_S1.1240.34
220_H230_F1.1210.34
40_F87_A1.1210.34
103_I653_S1.1200.34
394_I684_I1.1190.34
766_M775_Q1.1170.34
164_G295_L1.1150.33
269_L798_T1.1150.33
244_V265_A1.1140.33
361_S483_Q1.1060.33
704_I741_L1.1060.33
58_I495_S1.1030.33
542_I745_S1.1000.33
179_I668_L1.0980.33
610_D636_A1.0980.33
628_D636_A1.0980.33
631_G636_A1.0980.33
91_G497_E1.0940.33
279_K322_Q1.0920.32
37_S177_L1.0920.32
704_I781_L1.0890.32
509_E512_I1.0890.32
91_G761_W1.0880.32
237_S581_S1.0870.32
230_F242_V1.0860.32
273_L621_K1.0820.32
766_M800_V1.0810.32
216_M443_Q1.0810.32
432_Y750_E1.0800.32
698_G746_D1.0800.32
698_G747_G1.0800.32
470_K552_K1.0790.32
643_T722_I1.0780.32
745_S755_V1.0770.32
696_K721_G1.0760.32
172_I370_S1.0760.32
215_G244_V1.0750.32
301_A316_L1.0720.32
528_I608_S1.0720.32
404_K407_R1.0710.32
431_E454_E1.0700.31
686_D738_G1.0690.31
50_G745_S1.0680.31
48_V229_S1.0680.31
488_S621_K1.0670.31
135_S678_S1.0650.31
288_V736_K1.0640.31
609_G629_G1.0640.31
99_H748_I1.0620.31
185_V685_I1.0610.31
681_D746_D1.0600.31
681_D747_G1.0600.31
36_H221_I1.0590.31
324_I804_I1.0580.31
696_K795_D1.0570.31
696_K796_D1.0570.31
261_M294_S1.0540.31
198_M732_S1.0480.31
109_A526_V1.0470.31
610_D795_D1.0460.30
610_D796_D1.0460.30
628_D795_D1.0460.30
628_D796_D1.0460.30
631_G795_D1.0460.30
631_G796_D1.0460.30
113_F554_I1.0420.30
243_G743_M1.0400.30
257_N423_V1.0400.30
48_V90_A1.0390.30
50_G234_G1.0360.30
774_P801_V1.0310.30
237_S240_C1.0310.30
137_A311_L1.0280.30
639_E663_T1.0270.30
610_D721_G1.0270.30
628_D721_G1.0270.30
631_G721_G1.0270.30
137_A444_Q1.0220.29
38_L134_F1.0220.29
232_F282_A1.0200.29
558_L587_S1.0190.29
52_L394_I1.0180.29
315_C384_D1.0170.29
653_S748_I1.0160.29
488_S645_K1.0160.29
371_F625_A1.0150.29
610_D696_K1.0150.29
628_D696_K1.0150.29
631_G696_K1.0150.29
87_A91_G1.0120.29
63_L68_P1.0030.29
636_A681_D1.0010.29
72_A645_K0.9960.28
265_A786_I0.9950.28
588_T699_S0.9920.28
191_I194_I0.9920.28
614_M805_D0.9910.28
413_F417_Y0.9910.28
356_K425_L0.9910.28
173_F668_L0.9900.28
745_S800_V0.9890.28
146_A677_Y0.9860.28
131_V311_L0.9850.28
93_L543_P0.9830.28
328_V637_H0.9810.28
265_A313_A0.9800.28
93_L283_A0.9790.28
609_G746_D0.9770.28
609_G747_G0.9770.28
61_E289_G0.9760.28
265_A321_P0.9750.28
526_V703_F0.9730.27
164_G612_Y0.9720.27
361_S643_T0.9710.27
557_M639_E0.9690.27
65_F556_P0.9680.27
90_A638_F0.9670.27
105_A280_A0.9660.27
173_F334_G0.9660.27
618_G748_I0.9650.27
430_K776_E0.9650.27
209_A219_E0.9620.27
489_E799_V0.9620.27
272_G483_Q0.9600.27
678_S737_A0.9590.27
45_I804_I0.9590.27
68_P688_Q0.9580.27
531_L587_S0.9580.27
101_L280_A0.9560.27
681_D719_P0.9560.27
195_I489_E0.9540.27
231_S428_E0.9510.27
399_C681_D0.9500.27
446_C610_D0.9480.26
446_C628_D0.9480.26
446_C631_G0.9480.26
250_L586_G0.9470.26
593_V741_L0.9470.26
50_G776_E0.9450.26
111_F740_L0.9440.26
48_V324_I0.9430.26
80_K581_S0.9420.26
85_V214_Q0.9410.26
112_V218_A0.9410.26
345_Y348_K0.9400.26
162_E409_W0.9390.26
67_L678_S0.9390.26
218_A302_G0.9380.26
633_G665_N0.9370.26
411_Q737_A0.9370.26
615_M712_K0.9360.26
629_G640_S0.9350.26
134_F327_K0.9310.26
417_Y670_L0.9310.26
85_V227_V0.9270.26
352_V785_V0.9270.26
607_V722_I0.9270.26
130_I142_G0.9250.26
208_L720_I0.9240.26
136_M692_C0.9210.25
279_K406_N0.9210.25
82_F438_L0.9200.25
289_G701_P0.9190.25
412_N753_K0.9190.25
625_A644_I0.9190.25
446_C681_D0.9170.25
116_V288_V0.9160.25
436_R685_I0.9160.25
127_A328_V0.9150.25
556_P663_T0.9140.25
655_I688_Q0.9140.25
183_K689_D0.9140.25
111_F156_Y0.9140.25
221_I278_K0.9130.25
399_C609_G0.9110.25
642_E661_I0.9110.25
630_M698_G0.9100.25
579_G603_G0.9090.25
72_A239_G0.9090.25
680_L733_E0.9090.25
716_S723_I0.9090.25
416_T798_T0.9080.25
469_L643_T0.9060.25
307_L532_E0.9050.25
135_S748_I0.9050.25
562_L723_I0.9040.25
82_F322_Q0.9030.25
51_F150_V0.9030.25
429_E692_C0.9030.25
222_L790_S0.9020.25
126_K680_L0.9010.25
315_C713_V0.9000.25
89_L422_Q0.8960.24
124_R295_L0.8960.24
48_V539_E0.8950.24
739_D745_S0.8940.24
67_L113_F0.8930.24
591_Y704_I0.8930.24
89_L287_I0.8930.24
702_S742_I0.8890.24
629_G746_D0.8870.24
629_G747_G0.8870.24
528_I745_S0.8870.24
596_G602_K0.8870.24
472_K632_N0.8860.24
136_M485_L0.8860.24
189_L476_S0.8840.24
719_P795_D0.8840.24
719_P796_D0.8840.24
699_S744_M0.8830.24
66_A264_L0.8830.24
764_R768_G0.8820.24
310_S426_E0.8810.24
463_H805_D0.8810.24
71_G510_E0.8800.24
205_L447_S0.8800.24
698_G719_P0.8780.24
154_Y189_L0.8770.24
129_P313_A0.8770.24
245_V626_I0.8760.24
348_K591_Y0.8760.24
127_A781_L0.8730.24
139_A526_V0.8720.24
671_R697_V0.8720.24
250_L273_L0.8720.24
678_S727_D0.8710.24
347_R526_V0.8700.23
51_F626_I0.8700.23
477_R687_L0.8670.23
629_G719_P0.8650.23
514_E711_M0.8640.23
136_M714_Q0.8630.23
529_Y646_L0.8610.23
363_V442_F0.8600.23
136_M645_K0.8580.23
227_V232_F0.8570.23
397_H724_N0.8570.23
706_R735_L0.8570.23
222_L327_K0.8570.23
356_K615_M0.8560.23
272_G309_E0.8560.23
48_V745_S0.8550.23
231_S282_A0.8530.23
178_P643_T0.8500.23
597_A612_Y0.8490.23
328_V663_T0.8460.23
326_R329_A0.8460.23
68_P525_H0.8450.23
77_R591_Y0.8450.23
535_N616_E0.8440.23
183_K325_T0.8440.23
226_V597_A0.8440.23
250_L289_G0.8430.23
613_S757_N0.8420.22
563_E804_I0.8410.22
171_L658_K0.8400.22
221_I280_A0.8390.22
376_Q800_V0.8390.22
172_I791_G0.8380.22
438_L613_S0.8380.22
168_I615_M0.8370.22
199_I316_L0.8370.22
269_L682_L0.8340.22
216_M582_H0.8340.22
422_Q803_R0.8340.22
201_I226_V0.8330.22
95_I612_Y0.8330.22
279_K396_E0.8320.22
587_S676_I0.8320.22
196_C317_F0.8310.22
91_G707_G0.8300.22
544_F575_P0.8290.22
754_H757_N0.8270.22
163_A329_A0.8270.22
138_A231_S0.8260.22
480_V717_N0.8250.22
245_V266_F0.8230.22
274_L634_A0.8230.22
566_I741_L0.8230.22
323_S540_M0.8200.22
159_A613_S0.8190.22
698_G795_D0.8180.22
698_G796_D0.8180.22
218_A803_R0.8180.22
138_A142_G0.8180.22
260_Q690_A0.8170.22
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427_T668_L0.5040.13
166_S516_L0.5040.13
72_A373_T0.5030.13
199_I420_M0.5030.13
85_V205_L0.5020.13
233_I776_E0.5020.13
721_G739_D0.5010.13
394_I674_D0.5010.13
629_G633_G0.5000.13
156_Y502_R0.5000.13
520_G661_I0.5000.13
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3t91A 3 0.2745 100 0.807 Contact Map
3pu9A 2 0.2769 100 0.835 Contact Map
3f79A 2 0.283 100 0.851 Contact Map
3ke6A 2 0.2902 100 0.854 Contact Map
3zt9A 1 0.2322 100 0.863 Contact Map
3w42A 1 0.2394 100 0.867 Contact Map
3eq2A 2 0.4135 100 0.87 Contact Map
2pk0A 1 0.2503 100 0.872 Contact Map
2j82A 1 0.2334 100 0.873 Contact Map
4da1A 1 0.2479 99.9 0.88 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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