GREMLIN Database
PBPE - Penicillin-binding protein 4*
UniProt: P32959 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 451 (383)
Sequences: 3534 (2957)
Seq/√Len: 151.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
171_M179_T4.6381.00
51_L57_F4.1131.00
178_K183_L3.5781.00
77_K180_S3.5641.00
190_T244_S3.5251.00
70_G176_F3.4641.00
83_E101_R3.4111.00
84_D88_R3.1461.00
81_G84_D3.0701.00
31_G320_R2.8991.00
172_S175_D2.8951.00
76_E263_S2.7991.00
10_Q38_H2.7671.00
73_L176_F2.7281.00
84_D89_W2.6951.00
73_L180_S2.6921.00
36_Y40_F2.6711.00
263_S266_S2.6361.00
65_P252_F2.5741.00
27_L250_F2.5421.00
253_D322_T2.5341.00
24_G57_F2.4741.00
165_I176_F2.4471.00
68_A104_L2.4441.00
169_S176_F2.4201.00
162_A238_G2.4031.00
54_N247_S2.3741.00
44_E47_E2.3721.00
39_S53_T2.3321.00
176_F180_S2.3281.00
37_H247_S2.2861.00
92_G167_K2.2361.00
60_A306_W2.2001.00
297_G319_H2.1791.00
76_E266_S2.1111.00
55_S206_A2.0831.00
61_S64_K2.0821.00
74_L165_I2.0761.00
305_G310_S2.0631.00
256_L271_F2.0231.00
43_A49_R1.9841.00
85_K88_R1.9701.00
101_R286_F1.9661.00
10_Q40_F1.9561.00
128_V131_D1.9391.00
182_F242_V1.9381.00
163_V166_E1.9281.00
394_R406_E1.9251.00
25_T246_T1.9081.00
163_V167_K1.9071.00
27_L246_T1.8101.00
285_G290_L1.8061.00
70_G177_I1.8031.00
66_F242_V1.7991.00
42_Y48_K1.7921.00
69_L252_F1.7631.00
298_R318_D1.7261.00
187_M190_T1.7131.00
102_H148_N1.7081.00
59_L244_S1.7041.00
53_T247_S1.6811.00
39_S51_L1.6771.00
171_M176_F1.6411.00
377_S387_Q1.6221.00
372_S390_T1.6131.00
257_Y315_R1.6051.00
108_S157_G1.5971.00
179_T183_L1.5961.00
101_R269_S1.5571.00
100_I164_I1.5451.00
71_I161_L1.5421.00
24_G51_L1.5341.00
101_R274_V1.5291.00
25_T51_L1.5151.00
75_E82_Y1.5151.00
29_A250_F1.4901.00
69_L256_L1.4871.00
316_Y344_E1.4841.00
375_V390_T1.4791.00
189_E204_H1.4731.00
24_G328_N1.4451.00
52_K204_H1.4441.00
29_A322_T1.4421.00
175_D179_T1.4361.00
130_Q134_D1.4361.00
70_G169_S1.4351.00
42_Y47_E1.4331.00
171_M180_S1.4231.00
105_N274_V1.4131.00
25_T39_S1.4111.00
182_F190_T1.4081.00
269_S272_S1.4051.00
74_L169_S1.4051.00
14_E17_G1.4011.00
26_V325_Y1.3971.00
273_P283_D1.3961.00
72_I266_S1.3881.00
72_I82_Y1.3821.00
43_A55_S1.3731.00
182_F187_M1.3691.00
61_S153_Y1.3651.00
249_L326_L1.3641.00
249_L324_I1.3451.00
146_E149_E1.3421.00
64_K153_Y1.3311.00
70_G165_I1.3291.00
391_E396_Y1.3261.00
86_V103_L1.3211.00
165_I169_S1.3121.00
60_A311_T1.3031.00
253_D313_M1.2941.00
72_I75_E1.2921.00
68_A158_Y1.2731.00
84_D100_I1.2651.00
75_E269_S1.2571.00
98_V108_S1.2561.00
71_I104_L1.2521.00
192_V242_V1.2501.00
316_Y319_H1.2491.00
208_G309_Y1.2461.00
66_F182_F1.2451.00
390_T393_E1.2431.00
51_L55_S1.2411.00
171_M175_D1.2391.00
131_D134_D1.2251.00
66_F252_F1.2201.00
372_S375_V1.2161.00
71_I165_I1.2141.00
59_L311_T1.2131.00
25_T57_F1.2091.00
256_L288_W1.2071.00
374_Y415_F1.2051.00
299_I316_Y1.1961.00
61_S155_N1.1901.00
74_L176_F1.1881.00
75_E80_L1.1871.00
69_L181_I1.1871.00
253_D315_R1.1821.00
32_G254_Q1.1771.00
190_T248_D1.1711.00
62_L311_T1.1611.00
73_L77_K1.1601.00
87_D96_Q1.1581.00
173_Y238_G1.1581.00
190_T245_V1.1551.00
97_G146_E1.1461.00
325_Y340_L1.1431.00
377_S385_A1.1401.00
80_L100_I1.1391.00
14_E18_E1.1381.00
66_F187_M1.1251.00
89_W100_I1.1230.99
62_L313_M1.1220.99
158_Y161_L1.1200.99
264_K268_E1.1140.99
58_E306_W1.1070.99
284_Y302_H1.1060.99
293_S345_H1.1030.99
69_L262_I1.1010.99
375_V378_Y1.0980.99
54_N189_E1.0950.99
297_G316_Y1.0940.99
303_S312_M1.0900.99
262_I267_K1.0900.99
10_Q36_Y1.0880.99
104_L158_Y1.0850.99
28_A323_L1.0770.99
408_F416_F1.0750.99
27_L34_I1.0670.99
411_S414_R1.0650.99
64_K155_N1.0640.99
104_L161_L1.0580.99
371_Y415_F1.0560.99
409_P412_E1.0560.99
164_I168_A1.0550.99
173_Y192_V1.0540.99
89_W168_A1.0540.99
102_H107_T1.0530.99
30_E321_K1.0520.99
250_F322_T1.0480.99
391_E394_R1.0470.99
71_I80_L1.0460.99
12_L16_L1.0440.99
74_L77_K1.0420.99
257_Y322_T1.0400.99
71_I164_I1.0390.99
177_I182_F1.0370.99
45_M209_Y1.0350.99
246_T326_L1.0320.99
410_S416_F1.0170.99
44_E48_K1.0070.99
155_N239_D1.0020.99
370_I373_R1.0010.99
73_L261_F1.0000.99
290_L298_R0.9990.99
187_M244_S0.9970.99
297_G318_D0.9860.99
189_E248_D0.9850.99
13_F26_V0.9800.99
103_L157_G0.9790.99
177_I192_V0.9750.99
72_I270_A0.9730.99
293_S296_K0.9710.99
379_L385_A0.9680.98
81_G89_W0.9680.98
186_G251_R0.9660.98
100_I161_L0.9630.98
27_L324_I0.9570.98
44_E209_Y0.9550.98
189_E245_V0.9540.98
29_A253_D0.9500.98
107_T148_N0.9460.98
67_T104_L0.9450.98
100_I168_A0.9430.98
82_Y286_F0.9390.98
98_V147_P0.9330.98
169_S180_S0.9260.98
206_A309_Y0.9230.98
17_G40_F0.9230.98
212_D215_S0.9200.98
56_L191_R0.9190.98
65_P288_W0.9190.98
74_L79_I0.9190.98
37_H53_T0.9170.98
389_T398_E0.9110.98
371_Y374_Y0.9100.98
44_E49_R0.9080.98
10_Q14_E0.9080.98
374_Y413_T0.9060.98
187_M324_I0.8990.98
145_F150_G0.8970.98
15_T19_K0.8930.97
99_T102_H0.8930.97
256_L270_A0.8920.97
173_Y177_I0.8900.97
305_G311_T0.8850.97
391_E404_R0.8820.97
11_T15_T0.8780.97
154_S157_G0.8780.97
81_G100_I0.8770.97
106_H158_Y0.8730.97
86_V164_I0.8710.97
112_D154_S0.8710.97
257_Y320_R0.8700.97
39_S246_T0.8700.97
23_N329_K0.8700.97
178_K196_R0.8660.97
63_S242_V0.8650.97
112_D156_T0.8590.97
295_E345_H0.8580.97
155_N240_G0.8570.97
244_S248_D0.8570.97
59_L242_V0.8570.97
300_V315_R0.8570.97
13_F17_G0.8560.97
151_W284_Y0.8550.97
251_R254_Q0.8490.97
211_Y215_S0.8470.96
79_I168_A0.8440.96
37_H246_T0.8400.96
288_W315_R0.8380.96
407_L415_F0.8340.96
260_D264_K0.8340.96
237_Q241_T0.8330.96
62_L249_L0.8330.96
59_L63_S0.8330.96
44_E207_Y0.8310.96
155_N302_H0.8300.96
253_D324_I0.8260.96
73_L181_I0.8200.96
316_Y321_K0.8180.96
110_L156_T0.8170.96
187_M251_R0.8170.96
292_N296_K0.8140.96
270_A288_W0.8130.96
201_R204_H0.8120.96
270_A285_G0.8100.95
25_T326_L0.8080.95
366_I370_I0.8050.95
60_A240_G0.8050.95
409_P413_T0.8040.95
366_I369_A0.8020.95
316_Y348_F0.8010.95
43_A209_Y0.8000.95
46_T209_Y0.7980.95
34_I254_Q0.7980.95
387_Q398_E0.7970.95
66_F249_L0.7970.95
177_I190_T0.7960.95
255_A267_K0.7920.95
259_D264_K0.7900.95
246_T324_I0.7870.95
185_A255_A0.7840.95
100_I104_L0.7830.95
271_F300_V0.7810.95
367_D370_I0.7800.94
165_I173_Y0.7800.94
62_L65_P0.7790.94
86_V93_F0.7770.94
246_T250_F0.7760.94
314_I340_L0.7750.94
255_A261_F0.7730.94
108_S154_S0.7710.94
103_L161_L0.7710.94
291_Q301_S0.7700.94
258_Q320_R0.7670.94
82_Y104_L0.7640.94
398_E404_R0.7630.94
191_R243_N0.7630.94
63_S173_Y0.7610.94
172_S176_F0.7530.93
290_L300_V0.7520.93
60_A309_Y0.7510.93
256_L267_K0.7460.93
123_S126_I0.7440.93
311_T326_L0.7440.93
154_S302_H0.7430.93
25_T324_I0.7420.93
178_K182_F0.7400.93
60_A307_P0.7360.93
85_K97_G0.7360.93
410_S414_R0.7340.92
23_N45_M0.7330.92
131_D135_M0.7330.92
106_H157_G0.7320.92
312_M340_L0.7320.92
396_Y406_E0.7320.92
9_L343_A0.7300.92
80_L164_I0.7300.92
169_S172_S0.7300.92
132_I135_M0.7290.92
105_N286_F0.7290.92
63_S241_T0.7250.92
310_S329_K0.7230.92
86_V90_L0.7230.92
86_V100_I0.7230.92
276_L279_G0.7210.92
67_T238_G0.7190.92
315_R322_T0.7190.92
27_L322_T0.7170.92
21_Q211_Y0.7140.91
56_L243_N0.7130.91
34_I250_F0.7130.91
87_D91_P0.7110.91
386_A397_L0.7080.91
9_L13_F0.7080.91
342_A346_I0.7070.91
112_D117_F0.7050.91
389_T396_Y0.7030.91
102_H147_P0.7020.91
66_F177_I0.7010.91
409_P415_F0.7000.91
67_T162_A0.6990.91
158_Y287_G0.6970.90
102_H105_N0.6970.90
93_F160_L0.6920.90
70_G180_S0.6910.90
397_L405_L0.6900.90
287_G302_H0.6890.90
261_F267_K0.6870.90
67_T161_L0.6870.90
181_I185_A0.6860.90
254_Q258_Q0.6850.90
60_A308_G0.6830.90
54_N245_V0.6820.89
90_L163_V0.6800.89
11_T14_E0.6780.89
207_Y219_V0.6780.89
366_I409_P0.6770.89
162_A237_Q0.6760.89
61_S302_H0.6760.89
56_L245_V0.6750.89
289_V302_H0.6740.89
86_V89_W0.6740.89
284_Y288_W0.6730.89
66_F244_S0.6730.89
208_G211_Y0.6710.89
153_Y302_H0.6710.89
244_S249_L0.6690.89
371_Y390_T0.6690.89
86_V160_L0.6680.88
58_E243_N0.6640.88
85_K89_W0.6640.88
24_G39_S0.6620.88
259_D267_K0.6620.88
271_F288_W0.6590.88
174_A192_V0.6580.88
187_M248_D0.6570.88
158_Y284_Y0.6540.87
22_F327_S0.6520.87
53_T246_T0.6500.87
80_L168_A0.6470.87
49_R209_Y0.6470.87
205_Y309_Y0.6470.87
175_D178_K0.6470.87
101_R105_N0.6460.87
12_L342_A0.6450.87
302_H328_N0.6450.87
135_M138_N0.6440.87
59_L62_L0.6430.87
308_G330_E0.6430.87
291_Q299_I0.6410.86
26_V38_H0.6410.86
351_P355_P0.6400.86
34_I37_H0.6390.86
391_E398_E0.6390.86
29_A32_G0.6370.86
236_I306_W0.6340.86
63_S162_A0.6330.86
410_S413_T0.6300.85
340_L343_A0.6300.85
159_V236_I0.6300.85
64_K302_H0.6300.85
190_T251_R0.6290.85
241_T306_W0.6280.85
69_L73_L0.6280.85
89_W164_I0.6270.85
342_A345_H0.6270.85
340_L347_L0.6260.85
259_D271_F0.6260.85
72_I286_F0.6250.85
127_A131_D0.6250.85
284_Y287_G0.6240.85
162_A176_F0.6230.85
373_R413_T0.6220.85
188_N248_D0.6210.85
159_V239_D0.6200.85
29_A34_I0.6160.84
113_Y156_T0.6160.84
77_K176_F0.6150.84
9_L12_L0.6140.84
213_V216_E0.6130.84
29_A254_Q0.6130.84
65_P313_M0.6130.84
268_E272_S0.6130.84
80_L89_W0.6120.84
182_F192_V0.6110.84
42_Y49_R0.6100.84
398_E402_Q0.6100.84
61_S240_G0.6080.83
129_N174_A0.6080.83
151_W289_V0.6080.83
380_L383_G0.6070.83
374_Y378_Y0.6040.83
60_A239_D0.6030.83
397_L401_G0.6020.83
72_I76_E0.6020.83
239_D306_W0.6010.83
389_T404_R0.6010.83
183_L188_N0.6010.83
284_Y289_V0.6010.83
100_I105_N0.6010.83
42_Y45_M0.6000.83
202_I205_Y0.5970.82
82_Y101_R0.5950.82
262_I266_S0.5950.82
112_D239_D0.5930.82
273_P276_L0.5920.82
14_E40_F0.5910.82
90_L167_K0.5900.81
59_L326_L0.5880.81
371_Y375_V0.5880.81
63_S237_Q0.5870.81
299_I344_E0.5870.81
60_A241_T0.5870.81
269_S274_V0.5870.81
42_Y209_Y0.5860.81
75_E266_S0.5830.81
380_L384_T0.5830.81
396_Y404_R0.5820.81
399_I403_L0.5810.81
354_V357_R0.5800.80
111_P156_T0.5770.80
314_I323_L0.5760.80
133_V163_V0.5760.80
397_L407_L0.5750.80
177_I181_I0.5750.80
236_I240_G0.5730.80
25_T55_S0.5730.80
399_I402_Q0.5700.79
130_Q163_V0.5700.79
301_S314_I0.5690.79
162_A173_Y0.5670.79
134_D137_M0.5670.79
254_Q320_R0.5660.79
81_G88_R0.5660.79
113_Y159_V0.5660.79
347_L351_P0.5650.79
129_N166_E0.5640.79
259_D268_E0.5630.78
286_F291_Q0.5630.78
274_V286_F0.5600.78
93_F136_L0.5590.78
386_A399_I0.5570.78
166_E169_S0.5570.78
13_F16_L0.5570.78
98_V110_L0.5530.77
61_S304_G0.5530.77
87_D95_Y0.5510.77
253_D257_Y0.5490.77
144_Y152_M0.5490.77
30_E33_D0.5480.77
350_Q354_V0.5450.76
369_A374_Y0.5450.76
65_P284_Y0.5450.76
90_L93_F0.5440.76
387_Q390_T0.5420.76
155_N305_G0.5420.76
141_L144_Y0.5410.76
349_G355_P0.5400.76
375_V389_T0.5390.75
85_K100_I0.5390.75
369_A372_S0.5380.75
73_L262_I0.5370.75
397_L400_A0.5370.75
173_Y237_Q0.5360.75
70_G181_I0.5360.75
67_T70_G0.5360.75
63_S67_T0.5350.75
75_E84_D0.5330.75
288_W313_M0.5330.75
90_L145_F0.5320.74
231_V234_D0.5320.74
175_D183_L0.5320.74
341_K345_H0.5300.74
59_L240_G0.5300.74
123_S127_A0.5270.74
174_A238_G0.5270.74
75_E81_G0.5270.74
60_A63_S0.5260.74
260_D267_K0.5260.74
63_S192_V0.5260.74
271_F315_R0.5250.73
31_G34_I0.5250.73
67_T192_V0.5240.73
187_M249_L0.5240.73
74_L180_S0.5240.73
129_N132_I0.5240.73
92_G163_V0.5230.73
98_V102_H0.5230.73
26_V36_Y0.5230.73
127_A135_M0.5220.73
41_G55_S0.5220.73
183_L265_A0.5210.73
60_A243_N0.5200.73
206_A209_Y0.5200.73
267_K271_F0.5190.73
69_L267_K0.5190.73
319_H348_F0.5180.72
181_I255_A0.5170.72
145_F152_M0.5170.72
83_E99_T0.5170.72
364_K370_I0.5160.72
41_G328_N0.5150.72
371_Y395_L0.5120.72
384_T399_I0.5110.71
129_N163_V0.5100.71
112_D152_M0.5080.71
128_V133_V0.5070.71
391_E406_E0.5070.71
393_E396_Y0.5060.71
26_V323_L0.5050.71
182_F248_D0.5040.70
56_L189_E0.5040.70
270_A286_F0.5040.70
188_N367_D0.5030.70
111_P144_Y0.5030.70
211_Y214_H0.5020.70
75_E78_G0.5020.70
133_V159_V0.5020.70
346_I350_Q0.5020.70
249_L313_M0.5010.70
138_N141_L0.5000.70
247_S251_R0.5000.70
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1ei5A 2 0.949 100 0.245 Contact Map
2qmiA 5 0.9313 100 0.28 Contact Map
1yqsA 1 0.7494 100 0.345 Contact Map
2qz6A 1 0.694 100 0.346 Contact Map
3tg9A 2 0.7095 100 0.347 Contact Map
3wrzA 1 0.7162 100 0.347 Contact Map
4netA 1 0.7162 100 0.347 Contact Map
3w8kA 1 0.6984 100 0.349 Contact Map
4gdnA 1 0.7228 100 0.351 Contact Map
2drwA 3 0.7672 100 0.355 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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