GREMLIN Database
PPOX - Protoporphyrinogen oxidase
UniProt: P32397 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 470 (424)
Sequences: 15481 (12577)
Seq/√Len: 610.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
7_H36_E4.2321.00
9_V278_A4.1031.00
38_T251_Y3.7841.00
245_L248_T3.7291.00
7_H279_D3.7181.00
42_A255_K3.6841.00
46_V252_K3.4941.00
258_K269_E3.4591.00
40_V276_L3.1261.00
20_A282_I3.1241.00
283_V290_A3.1021.00
46_V242_E2.9391.00
9_V40_V2.7951.00
44_P253_G2.7771.00
269_E275_T2.7511.00
457_G461_V2.7081.00
260_S269_E2.6991.00
235_Q239_E2.6801.00
9_V276_L2.6781.00
13_G46_V2.6591.00
36_E249_K2.5341.00
9_V268_L2.4051.00
236_T239_E2.3601.00
330_T334_I2.3541.00
379_S382_D2.3151.00
257_T271_D2.3051.00
11_I283_V2.3011.00
255_K271_D2.3011.00
254_T270_L2.2931.00
270_L276_L2.2901.00
331_G334_I2.2751.00
251_Y274_V2.2641.00
8_V35_L2.2631.00
40_V251_Y2.2311.00
23_Y458_K2.2271.00
242_E246_K2.1991.00
284_T441_T2.1791.00
242_E250_V2.1771.00
150_Q158_R2.1741.00
54_K59_I2.1281.00
251_Y254_T2.1241.00
11_I285_A2.1061.00
280_S439_Y2.0821.00
52_T59_I2.0341.00
7_H38_T2.0291.00
267_S277_D2.0141.00
45_R51_Q2.0021.00
15_I450_I1.9991.00
117_K120_P1.9901.00
319_P400_E1.9551.00
16_T284_T1.9461.00
319_P402_E1.9331.00
153_G157_R1.9301.00
23_Y26_K1.8941.00
22_F244_Q1.8781.00
40_V278_A1.8771.00
240_E243_K1.8701.00
384_I404_T1.8361.00
27_E465_L1.8161.00
260_S267_S1.8161.00
19_A454_I1.8091.00
267_S275_T1.8071.00
6_K38_T1.7861.00
257_T269_E1.7861.00
262_S267_S1.7461.00
385_N389_E1.7391.00
254_T272_N1.7321.00
282_I464_A1.7141.00
381_N385_N1.7051.00
23_Y462_S1.6701.00
260_S275_T1.6691.00
26_K80_D1.6261.00
151_S154_E1.6051.00
360_K402_E1.6051.00
38_T249_K1.5981.00
115_P130_S1.5831.00
392_K397_I1.5811.00
8_V280_S1.5801.00
411_E414_P1.5631.00
251_Y276_L1.5591.00
270_L274_V1.5451.00
285_A290_A1.5431.00
35_L465_L1.5251.00
21_A39_L1.5201.00
380_D408_R1.5091.00
246_K249_K1.4931.00
8_V24_M1.4921.00
329_G332_F1.4591.00
79_K84_E1.4531.00
50_I238_V1.4451.00
331_G335_S1.4421.00
46_V238_V1.4361.00
9_V38_T1.4291.00
266_Y281_V1.4261.00
441_T460_A1.4231.00
254_T274_V1.4221.00
414_P418_V1.4211.00
76_Q80_D1.4111.00
58_Y317_G1.4091.00
25_E248_T1.3981.00
17_G284_T1.3821.00
46_V250_V1.3811.00
239_E243_K1.3801.00
38_T276_L1.3791.00
22_F26_K1.3711.00
40_V256_V1.3681.00
430_A433_S1.3521.00
10_I21_A1.3421.00
439_Y460_A1.3421.00
39_L250_V1.3411.00
153_G182_D1.3271.00
134_R137_M1.3241.00
245_L250_V1.3201.00
280_S464_A1.3181.00
86_L231_S1.3161.00
7_H35_L1.3141.00
330_T333_V1.3141.00
162_D166_E1.3091.00
243_K246_K1.3091.00
234_L450_I1.3071.00
180_D183_K1.3071.00
38_T278_A1.3031.00
129_L132_K1.2891.00
157_R166_E1.2811.00
75_P79_K1.2741.00
40_V254_T1.2711.00
259_L266_Y1.2561.00
49_K443_A1.2561.00
332_F335_S1.2451.00
122_V125_G1.2401.00
412_S415_Q1.2331.00
278_A281_V1.2301.00
261_H266_Y1.2271.00
16_T456_Q1.2261.00
384_I388_L1.2191.00
314_V406_V1.2171.00
401_P404_T1.2161.00
22_F241_I1.2141.00
459_A463_D1.2091.00
460_A463_D1.2061.00
256_V268_L1.2021.00
319_P360_K1.2011.00
390_D393_K1.1911.00
318_F388_L1.1891.00
286_P289_A1.1861.00
25_E245_L1.1861.00
266_Y437_G1.1851.00
287_H444_S1.1791.00
76_Q458_K1.1761.00
125_G128_S1.1731.00
25_E37_L1.1721.00
54_K57_G1.1711.00
73_S458_K1.1671.00
317_G403_M1.1651.00
318_F401_P1.1641.00
316_L388_L1.1591.00
84_E231_S1.1481.00
16_T441_T1.1471.00
411_E419_G1.1461.00
13_G39_L1.1421.00
54_K235_Q1.1411.00
386_I389_E1.1381.00
366_Y369_K1.1261.00
230_L237_L1.1241.00
77_L81_L1.1241.00
40_V270_L1.1241.00
413_M419_G1.1221.00
115_P118_I1.1221.00
76_Q79_K1.1191.00
388_L392_K1.1081.00
329_G333_V1.1071.00
232_T236_T1.1061.00
6_K279_D1.1041.00
347_W350_K1.0941.00
262_S277_D1.0931.00
445_F459_A1.0891.00
320_E400_E1.0891.00
254_T276_L1.0891.00
458_K462_S1.0831.00
414_P419_G1.0811.00
414_P417_H1.0761.00
410_H413_M1.0731.00
328_E331_G1.0711.00
53_V62_R1.0641.00
126_L132_K1.0621.00
331_G336_R1.0561.00
382_D385_N1.0551.00
150_Q154_E1.0511.00
265_C277_D1.0501.00
262_S265_C1.0501.00
23_Y457_G1.0461.00
413_M417_H1.0421.00
364_R368_G1.0391.00
242_E252_K1.0391.00
239_E242_E1.0361.00
84_E87_L1.0311.00
170_E181_I1.0291.00
24_M461_V1.0241.00
171_P175_G1.0231.00
137_M141_L1.0191.00
411_E418_V1.0191.00
259_L281_V1.0151.00
93_G96_Y1.0131.00
439_Y464_A1.0111.00
382_D386_I1.0101.00
259_L283_V1.0071.00
22_F245_L1.0061.00
10_I37_L1.0041.00
432_A463_D1.0031.00
406_V409_W1.0021.00
113_G116_T0.9971.00
118_I122_V0.9961.00
289_A292_G0.9961.00
389_E393_K0.9931.00
244_Q247_L0.9891.00
23_Y80_D0.9881.00
445_F456_Q0.9881.00
174_S180_D0.9871.00
412_S416_Y0.9861.00
138_D141_L0.9861.00
13_G252_K0.9851.00
7_H276_L0.9841.00
55_K405_C0.9831.00
236_T240_E0.9781.00
344_A348_T0.9771.00
163_E167_N0.9721.00
78_V83_L0.9661.00
410_H414_P0.9651.00
126_L129_L0.9601.00
415_Q418_V0.9581.00
112_M115_P0.9551.00
418_V421_K0.9551.00
113_G117_K0.9521.00
15_I50_I0.9471.00
415_Q419_G0.9471.00
413_M418_V0.9451.00
127_F131_G0.9411.00
439_Y463_D0.9361.00
22_F80_D0.9361.00
435_Y438_V0.9351.00
378_L382_D0.9351.00
26_K244_Q0.9341.00
74_A454_I0.9331.00
35_L38_T0.9271.00
128_S131_G0.9261.00
7_H277_D0.9261.00
329_G334_I0.9261.00
342_I347_W0.9201.00
52_T235_Q0.9181.00
419_G422_Q0.9181.00
412_S417_H0.9151.00
79_K82_G0.9151.00
367_V387_V0.9111.00
343_T346_T0.9101.00
115_P121_F0.9091.00
6_K36_E0.9051.00
243_K247_L0.9001.00
403_M409_W0.8981.00
122_V126_L0.8981.00
411_E422_Q0.8911.00
20_A457_G0.8901.00
343_T348_T0.8851.00
43_S51_Q0.8811.00
135_A139_F0.8771.00
411_E416_Y0.8761.00
114_I117_K0.8741.00
23_Y27_E0.8731.00
391_L395_M0.8731.00
283_V438_V0.8721.00
270_L273_G0.8711.00
238_V242_E0.8711.00
285_A293_M0.8701.00
78_V81_L0.8701.00
58_Y357_P0.8681.00
268_L271_D0.8671.00
344_A347_W0.8661.00
81_L85_H0.8651.00
132_K135_A0.8641.00
340_F393_K0.8631.00
128_S132_K0.8611.00
389_E392_K0.8611.00
317_G402_E0.8591.00
401_P409_W0.8581.00
417_H420_H0.8541.00
261_H435_Y0.8491.00
167_N171_P0.8471.00
261_H437_G0.8461.00
107_P113_G0.8431.00
175_G178_A0.8421.00
361_T364_R0.8411.00
6_K9_V0.8401.00
83_L247_L0.8351.00
191_P194_Y0.8341.00
49_K63_G0.8311.00
116_T120_P0.8291.00
118_I121_F0.8271.00
171_P174_S0.8221.00
170_E174_S0.8211.00
442_G456_Q0.8211.00
390_D394_V0.8191.00
123_S129_L0.8151.00
56_D403_M0.8151.00
333_V336_R0.8141.00
55_K403_M0.8131.00
380_D384_I0.8101.00
411_E420_H0.8091.00
414_P420_H0.8081.00
129_L133_A0.8081.00
330_T335_S0.8061.00
34_P37_L0.8061.00
175_G179_G0.8021.00
73_S76_Q0.8021.00
342_I348_T0.8021.00
114_I118_I0.7971.00
316_L365_A0.7971.00
177_Y184_L0.7951.00
453_C457_G0.7941.00
21_A245_L0.7921.00
25_E249_K0.7911.00
136_A140_I0.7901.00
92_T95_S0.7891.00
157_R162_D0.7881.00
371_G374_S0.7881.00
343_T347_W0.7851.00
39_L245_L0.7851.00
53_V405_C0.7841.00
58_Y360_K0.7821.00
412_S419_G0.7811.00
392_K398_N0.7791.00
328_E334_I0.7791.00
27_E34_P0.7761.00
461_V464_A0.7751.00
16_T453_C0.7741.00
259_L268_L0.7691.00
19_A23_Y0.7661.00
27_E462_S0.7621.00
460_A464_A0.7611.00
341_A347_W0.7591.00
312_A408_R0.7591.00
116_T119_A0.7581.00
186_L190_F0.7581.00
257_T260_S0.7581.00
82_G88_V0.7581.00
15_I238_V0.7551.00
61_E234_L0.7531.00
38_T279_D0.7531.00
77_L80_D0.7511.00
7_H278_A0.7511.00
257_T270_L0.7511.00
131_G134_R0.7511.00
189_T192_Q0.7511.00
81_L241_I0.7511.00
119_A122_V0.7501.00
130_S134_R0.7491.00
318_F322_S0.7491.00
19_A457_G0.7451.00
280_S437_G0.7451.00
361_T365_A0.7421.00
73_S455_D0.7391.00
391_L398_N0.7351.00
50_I64_P0.7341.00
384_I387_V0.7331.00
10_I17_G0.7321.00
240_E244_Q0.7281.00
187_M190_F0.7281.00
185_S188_S0.7251.00
111_V115_P0.7231.00
6_K35_L0.7221.00
27_E35_L0.7191.00
131_G135_A0.7191.00
96_Y99_V0.7191.00
11_I256_V0.7181.00
15_I453_C0.7171.00
83_L88_V0.7161.00
150_Q155_F0.7161.00
123_S132_K0.7161.00
163_E166_E0.7151.00
381_N384_I0.7151.00
75_P85_H0.7141.00
256_V270_L0.7131.00
372_D375_I0.7131.00
85_H88_V0.7121.00
365_A391_L0.7121.00
402_E405_C0.7111.00
383_I406_V0.7111.00
144_S148_D0.7101.00
455_D459_A0.7101.00
312_A406_V0.7071.00
75_P228_Q0.7071.00
115_P119_A0.7061.00
112_M116_T0.7001.00
115_P122_V0.6981.00
36_E279_D0.6981.00
348_T352_W0.6981.00
132_K136_A0.6971.00
288_K292_G0.6961.00
105_P108_K0.6961.00
15_I19_A0.6961.00
169_I172_L0.6951.00
413_M416_Y0.6931.00
285_A289_A0.6911.00
136_A139_F0.6901.00
94_Q98_L0.6901.00
257_T273_G0.6891.00
21_A248_T0.6881.00
109_G113_G0.6881.00
282_I439_Y0.6871.00
386_I390_D0.6841.00
25_E246_K0.6841.00
95_S98_L0.6821.00
441_T457_G0.6821.00
364_R369_K0.6801.00
15_I454_I0.6801.00
172_L175_G0.6791.00
405_C409_W0.6791.00
405_C408_R0.6761.00
388_L401_P0.6761.00
414_P421_K0.6761.00
321_G324_Q0.6751.00
192_Q195_Q0.6741.00
134_R138_D0.6741.00
413_M420_H0.6721.00
137_M140_I0.6721.00
119_A123_S0.6701.00
71_K451_P0.6691.00
45_R235_Q0.6671.00
162_D165_V0.6661.00
328_E333_V0.6661.00
119_A124_T0.6631.00
125_G132_K0.6631.00
104_H109_G0.6631.00
77_L454_I0.6621.00
400_E403_M0.6611.00
135_A138_D0.6611.00
191_P195_Q0.6601.00
329_G335_S0.6591.00
416_Y419_G0.6561.00
35_L279_D0.6551.00
133_A140_I0.6551.00
282_I460_A0.6521.00
22_F81_L0.6501.00
64_P234_L0.6491.00
428_R431_L0.6491.00
384_I406_V0.6481.00
187_M191_P0.6471.00
123_S126_L0.6471.00
127_F132_K0.6471.00
143_A146_T0.6461.00
95_S105_P0.6451.00
152_L156_F0.6431.00
127_F130_S0.6401.00
41_E252_K0.6381.00
40_V268_L0.6381.00
429_E433_S0.6371.00
119_A125_G0.6371.00
18_L245_L0.6371.00
230_L236_T0.6331.00
106_M109_G0.6331.00
412_S418_V0.6331.00
287_H291_A0.6321.00
342_I345_C0.6301.00
404_T408_R0.6301.00
461_V465_L0.6291.00
312_A315_A0.6291.00
341_A344_A0.6291.00
172_L177_Y0.6281.00
363_L368_G0.6281.00
316_L404_T0.6271.00
102_T164_V0.6261.00
164_V167_N0.6251.00
178_A185_S0.6251.00
171_P176_I0.6251.00
309_T313_N0.6251.00
301_S304_K0.6241.00
404_T409_W0.6231.00
132_K137_M0.6231.00
50_I234_L0.6221.00
18_L238_V0.6221.00
188_S191_P0.6221.00
111_V114_I0.6211.00
181_I184_L0.6201.00
9_V281_V0.6191.00
82_G85_H0.6191.00
35_L280_S0.6191.00
428_R446_E0.6181.00
82_G87_L0.6161.00
78_V230_L0.6151.00
133_A139_F0.6121.00
177_Y189_T0.6121.00
418_V422_Q0.6101.00
15_I241_I0.6081.00
117_K121_F0.6081.00
143_A147_K0.6071.00
281_V437_G0.6071.00
265_C436_P0.6061.00
317_G360_K0.6061.00
356_A359_G0.6051.00
58_Y402_E0.6041.00
385_N388_L0.6041.00
133_A136_A0.6041.00
412_S422_Q0.6001.00
385_N404_T0.6001.00
20_A460_A0.6001.00
286_P444_S0.5991.00
76_Q81_L0.5991.00
303_L306_M0.5981.00
154_E157_R0.5961.00
306_M309_T0.5961.00
148_D151_S0.5951.00
416_Y420_H0.5941.00
19_A241_I0.5941.00
344_A349_N0.5891.00
287_H303_L0.5891.00
187_M193_F0.5881.00
265_C279_D0.5881.00
404_T407_T0.5871.00
415_Q420_H0.5871.00
18_L39_L0.5871.00
24_M464_A0.5851.00
126_L130_S0.5851.00
392_K399_G0.5831.00
74_A450_I0.5811.00
383_I387_V0.5811.00
125_G136_A0.5801.00
119_A126_L0.5801.00
395_M398_N0.5791.00
268_L281_V0.5781.00
105_P109_G0.5781.00
75_P78_V0.5771.00
177_Y181_I0.5771.00
316_L401_P0.5771.00
52_T61_E0.5751.00
6_K280_S0.5741.00
61_E66_S0.5731.00
190_F194_Y0.5731.00
65_D364_R0.5731.00
412_S421_K0.5731.00
174_S181_I0.5711.00
388_L404_T0.5701.00
125_G129_L0.5701.00
318_F323_V0.5701.00
179_G183_K0.5691.00
340_F390_D0.5691.00
81_L86_L0.5681.00
50_I450_I0.5671.00
129_L134_R0.5671.00
459_A462_S0.5671.00
380_D383_I0.5661.00
120_P123_S0.5641.00
411_E415_Q0.5631.00
123_S127_F0.5631.00
184_L188_S0.5631.00
112_M130_S0.5611.00
184_L189_T0.5611.00
189_T195_Q0.5611.00
280_S283_V0.5611.00
10_I282_I0.5611.00
186_L189_T0.5601.00
64_P450_I0.5601.00
51_Q407_T0.5591.00
417_H422_Q0.5591.00
84_E88_V0.5581.00
311_V315_A0.5581.00
60_I362_L0.5551.00
166_E170_E0.5551.00
410_H418_V0.5541.00
375_I378_L0.5521.00
261_H436_P0.5521.00
92_T98_L0.5500.99
289_A293_M0.5500.99
360_K364_R0.5490.99
74_A78_V0.5480.99
342_I346_T0.5480.99
283_V440_M0.5480.99
94_Q99_V0.5470.99
413_M422_Q0.5460.99
39_L46_V0.5460.99
314_V317_G0.5450.99
130_S133_A0.5450.99
174_S177_Y0.5440.99
114_I121_F0.5430.99
332_F336_R0.5430.99
356_A360_K0.5420.99
302_H306_M0.5420.99
6_K249_K0.5410.99
348_T351_K0.5410.99
314_V318_F0.5400.99
341_A349_N0.5400.99
92_T97_V0.5390.99
97_V105_P0.5390.99
346_T351_K0.5390.99
349_N361_T0.5370.99
242_E248_T0.5370.99
174_S178_A0.5370.99
328_E332_F0.5360.99
180_D185_S0.5320.99
164_V171_P0.5320.99
132_K138_D0.5310.99
290_A294_L0.5300.99
373_E376_V0.5280.99
189_T193_F0.5270.99
410_H415_Q0.5260.99
188_S192_Q0.5230.99
258_K295_S0.5220.99
94_Q97_V0.5210.99
122_V127_F0.5210.99
23_Y77_L0.5210.99
73_S454_I0.5200.99
362_L368_G0.5190.99
261_H438_V0.5170.99
160_V169_I0.5170.99
72_K76_Q0.5160.99
171_P177_Y0.5150.99
147_K150_Q0.5150.99
155_F186_L0.5150.99
80_D83_L0.5150.99
417_H421_K0.5130.99
229_T234_L0.5130.99
287_H306_M0.5130.99
328_E335_S0.5130.99
103_L107_P0.5130.99
344_A351_K0.5130.99
372_D376_V0.5120.99
132_K140_I0.5110.99
131_G138_D0.5110.99
282_I441_T0.5100.99
177_Y180_D0.5100.99
73_S451_P0.5090.99
37_L245_L0.5070.99
51_Q62_R0.5050.99
172_L176_I0.5030.99
175_G180_D0.5010.99
365_A369_K0.5010.99
380_D406_V0.5000.99
292_G304_K0.5000.99
149_D154_E0.5000.99
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3i6dA 1 0.8787 100 0.285 Contact Map
3lovA 1 0.9447 100 0.319 Contact Map
2ivdA 1 0.9383 100 0.332 Contact Map
1sezA 3 0.9404 100 0.34 Contact Map
4i59A 1 0.9043 100 0.366 Contact Map
2yg5A 2 0.9085 100 0.367 Contact Map
3nksA 2 0.934 100 0.39 Contact Map
1s3eA 2 0.9128 100 0.39 Contact Map
4dsgA 1 0.9213 100 0.421 Contact Map
2vvmA 2 0.9021 100 0.422 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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