GREMLIN Database
HEMH - Ferrochelatase
UniProt: P32396 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 310 (292)
Sequences: 3152 (1926)
Seq/√Len: 112.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
155_T210_G3.7101.00
70_H298_D3.5101.00
182_A204_A3.2901.00
60_T84_I3.0561.00
187_E191_E3.0181.00
62_Q292_A2.9811.00
83_Y99_E2.9031.00
177_M217_A2.9001.00
229_P232_G2.7871.00
274_K278_D2.6101.00
150_E306_K2.5901.00
66_N294_P2.5841.00
146_S199_Q2.4721.00
128_R131_E2.4131.00
17_Y90_E2.4071.00
187_E229_P2.3701.00
249_Q283_S2.3481.00
159_D295_E2.2711.00
239_T251_F2.2091.00
98_A102_K2.1741.00
18_K21_D2.1451.00
70_H301_A2.1211.00
163_E293_K2.0791.00
208_A216_Y2.0771.00
241_D246_K2.0431.00
11_M125_Y2.0421.00
63_Q297_I1.9821.00
188_K228_D1.9561.00
83_Y103_D1.9401.00
83_Y100_M1.9401.00
149_D202_E1.9121.00
190_K197_P1.8901.00
107_E140_T1.8641.00
162_K210_G1.8271.00
255_P269_N1.8091.00
152_K302_T1.7581.00
204_A218_V1.7321.00
205_K209_E1.7171.00
238_L241_D1.7101.00
66_N297_I1.6811.00
202_E206_L1.6651.00
6_M107_E1.6461.00
161_V254_V1.6351.00
87_K124_S1.6261.00
219_G235_V1.6221.00
81_K103_D1.6051.00
180_V207_I1.6041.00
197_P201_H1.5781.00
67_L297_I1.5651.00
110_S129_A1.5581.00
90_E95_D1.5571.00
198_D202_E1.5421.00
57_A61_E1.5341.00
202_E205_K1.5301.00
53_I58_Q1.5301.00
201_H220_W1.5291.00
237_D240_R1.4841.00
27_T37_P1.4641.00
180_V254_V1.4461.00
182_A218_V1.4441.00
180_V216_Y1.4441.00
52_G62_Q1.4331.00
147_W259_V1.4331.00
131_E134_E1.4281.00
217_A238_L1.4021.00
15_T57_A1.3931.00
37_P40_L1.3901.00
99_E103_D1.3901.00
153_F157_W1.3851.00
270_D286_R1.3831.00
154_V210_G1.3781.00
157_W207_I1.3421.00
22_I43_L1.3401.00
58_Q62_Q1.3351.00
59_I260_A1.3291.00
142_T307_K1.3201.00
7_G105_I1.3181.00
66_N292_A1.3171.00
96_A99_E1.3040.99
236_Q276_V1.3010.99
185_L189_I1.2950.99
160_R293_K1.2930.99
239_T273_C1.2900.99
85_G96_A1.2760.99
185_L190_K1.2730.99
7_G96_A1.2560.99
17_Y24_R1.2530.99
219_G238_L1.2530.99
101_H136_L1.2500.99
83_Y96_A1.2480.99
150_E303_V1.2480.99
65_H69_Q1.2460.99
164_T285_Y1.2450.99
204_A216_Y1.2450.99
126_N143_S1.2430.99
197_P220_W1.2420.99
158_V210_G1.2320.99
96_A100_M1.2300.99
28_H128_R1.2300.99
275_V278_D1.2300.99
100_M108_A1.2300.99
151_P210_G1.2290.99
165_Y252_V1.2210.99
252_V285_Y1.2160.99
223_E234_D1.2160.99
159_D162_K1.2150.99
180_V208_A1.2140.99
155_T159_D1.2060.99
27_T31_R1.2040.99
274_K284_Y1.1980.99
7_G100_M1.1980.99
158_V162_K1.1950.99
111_I300_L1.1720.99
250_A283_S1.1630.99
154_V206_L1.1600.99
164_T167_S1.1590.99
240_R276_V1.1580.99
67_L301_A1.1560.99
157_W290_P1.1560.99
64_A68_E1.1500.99
148_Y199_Q1.1480.99
38_E42_D1.1480.99
301_A305_L1.1450.99
190_K198_D1.1410.99
52_G58_Q1.1410.99
299_A303_V1.1310.99
206_L209_E1.1280.99
259_V296_F1.1250.98
68_E72_N1.1250.98
28_H89_I1.1180.98
107_E142_T1.1170.98
36_E40_L1.1150.98
240_R279_D1.1100.98
59_I292_A1.1060.98
100_M105_I1.1030.98
65_H68_E1.1010.98
45_D48_E1.0970.98
261_D291_N1.0960.98
61_E65_H1.0940.98
266_L270_D1.0900.98
41_Q45_D1.0750.98
64_A84_I1.0730.98
98_A136_L1.0730.98
176_A249_Q1.0600.98
204_A208_A1.0560.98
152_K298_D1.0460.98
150_E302_T1.0450.98
253_Y284_Y1.0440.98
170_E215_E1.0410.97
98_A132_E1.0400.97
273_C276_V1.0400.97
17_Y21_D1.0290.97
105_I108_A1.0290.97
275_V279_D1.0280.97
179_I251_F1.0200.97
66_N69_Q1.0140.97
147_W296_F1.0120.97
181_S221_Q1.0110.97
126_N130_K1.0100.97
148_Y206_L1.0080.97
57_A84_I1.0040.97
177_M248_Y1.0020.97
188_K192_F0.9990.97
67_L300_L0.9950.97
152_K155_T0.9820.96
158_V207_I0.9790.96
175_N249_Q0.9730.96
140_T307_K0.9720.96
160_R288_E0.9720.96
179_I217_A0.9710.96
70_H73_E0.9700.96
109_V304_V0.9660.96
11_M93_I0.9580.96
93_I125_Y0.9520.96
255_P286_R0.9470.96
232_G237_D0.9430.96
277_T282_A0.9410.95
157_W254_V0.9390.95
272_E276_V0.9360.95
120_F185_L0.9350.95
28_H92_F0.9310.95
161_V178_L0.9220.95
19_E22_I0.9220.95
71_L301_A0.9200.95
5_K106_T0.9200.95
9_L85_G0.9170.95
156_Y290_P0.9100.95
155_T295_E0.9080.94
111_I144_V0.9020.94
49_A271_Y0.8980.94
6_M109_V0.8920.94
161_V207_I0.8900.94
175_N250_A0.8880.94
109_V144_V0.8860.94
9_L96_A0.8850.94
128_R132_E0.8830.94
239_T280_I0.8830.94
165_Y170_E0.8820.94
98_A101_H0.8790.93
100_M103_D0.8760.93
116_H200_L0.8710.93
132_E136_L0.8640.93
149_D206_L0.8590.93
109_V307_K0.8570.93
118_S189_I0.8540.92
176_A250_A0.8540.92
110_S143_S0.8530.92
243_F280_I0.8500.92
240_R245_Q0.8460.92
167_S285_Y0.8450.92
38_E41_Q0.8440.92
93_I128_R0.8430.92
63_Q300_L0.8400.92
162_K166_A0.8400.92
176_A252_V0.8340.92
23_E40_L0.8340.92
198_D201_H0.8300.91
152_K295_E0.8290.91
276_V279_D0.8290.91
243_F282_A0.8280.91
154_V203_S0.8270.91
270_D274_K0.8270.91
163_E167_S0.8210.91
61_E84_I0.8180.91
269_N284_Y0.8170.91
8_L304_V0.8150.91
94_E132_E0.8150.91
160_R163_E0.8110.90
23_E37_P0.8100.90
178_L254_V0.8080.90
113_L259_V0.8070.90
243_F247_G0.8040.90
59_I62_Q0.8040.90
157_W161_V0.7960.90
19_E48_E0.7950.90
59_I291_N0.7950.90
37_P41_Q0.7930.89
180_V204_A0.7930.89
243_F246_K0.7920.89
94_E135_K0.7910.89
198_D205_K0.7910.89
87_K128_R0.7900.89
179_I219_G0.7870.89
133_A136_L0.7800.89
49_A267_Y0.7750.88
240_R280_I0.7740.88
109_V142_T0.7730.88
25_Y43_L0.7700.88
7_G83_Y0.7700.88
62_Q65_H0.7670.88
157_W203_S0.7660.88
177_M246_K0.7650.88
72_N79_T0.7620.87
152_K299_A0.7620.87
157_W160_R0.7610.87
182_A200_L0.7600.87
130_K143_S0.7590.87
132_E135_K0.7570.87
253_Y269_N0.7540.87
164_T288_E0.7540.87
243_F249_Q0.7520.87
243_F248_Y0.7500.87
19_E44_K0.7490.87
120_F189_I0.7450.86
111_I304_V0.7400.86
203_S207_I0.7380.86
241_D245_Q0.7360.86
239_T284_Y0.7350.86
238_L242_L0.7350.86
17_Y61_E0.7320.85
97_V101_H0.7280.85
7_G85_G0.7240.85
25_Y28_H0.7220.85
159_D163_E0.7190.84
201_H218_V0.7170.84
302_T306_K0.7160.84
301_A304_V0.7130.84
97_V132_E0.7100.84
72_N80_F0.7090.83
296_F299_A0.7060.83
35_P39_M0.7050.83
163_E288_E0.7040.83
108_A139_L0.6990.83
34_K127_K0.6980.82
154_V207_I0.6970.82
273_C289_M0.6960.82
189_I228_D0.6930.82
10_V64_A0.6920.82
175_N252_V0.6900.82
22_I44_K0.6880.82
123_Q127_K0.6880.82
64_A82_A0.6870.81
97_V108_A0.6840.81
68_E81_K0.6830.81
46_R267_Y0.6770.81
13_Y114_A0.6750.80
168_M176_A0.6710.80
92_F95_D0.6710.80
95_D98_A0.6700.80
62_Q66_N0.6680.80
140_T145_E0.6680.80
165_Y178_L0.6680.80
298_D302_T0.6670.80
163_E166_A0.6670.80
164_T168_M0.6660.79
243_F283_S0.6660.79
60_T64_A0.6640.79
119_T123_Q0.6630.79
251_F282_A0.6620.79
154_V158_V0.6620.79
206_L210_G0.6590.79
201_H205_K0.6590.79
168_M285_Y0.6580.79
11_M129_A0.6570.79
208_A213_V0.6520.78
296_F300_L0.6510.78
252_V287_P0.6500.78
271_Y275_V0.6490.78
50_I267_Y0.6470.77
148_Y202_E0.6460.77
179_I238_L0.6440.77
15_T54_S0.6420.77
261_D289_M0.6420.77
270_D273_C0.6410.77
32_G88_H0.6390.77
101_H133_A0.6390.77
168_M250_A0.6380.76
136_L139_L0.6360.76
67_L304_V0.6330.76
213_V216_Y0.6330.76
273_C284_Y0.6330.76
13_Y29_I0.6330.76
8_L80_F0.6310.76
42_D46_R0.6310.76
197_P231_L0.6290.75
116_H121_S0.6280.75
177_M215_E0.6280.75
177_M251_F0.6270.75
150_E299_A0.6250.75
29_I262_H0.6240.75
97_V136_L0.6210.75
276_V280_I0.6200.74
160_R254_V0.6190.74
165_Y175_N0.6190.74
5_K81_K0.6170.74
226_T268_D0.6160.74
94_E131_E0.6150.74
28_H88_H0.6140.74
298_D301_A0.6140.74
9_L97_V0.6140.74
81_K85_G0.6100.73
25_Y29_I0.6090.73
99_E102_K0.6070.73
97_V129_A0.6070.73
165_Y168_M0.6060.73
297_I300_L0.6050.73
46_R263_L0.6040.73
176_A248_Y0.6030.72
236_Q273_C0.6010.72
84_I96_A0.6000.72
18_K53_I0.5980.72
26_Y39_M0.5980.72
6_M80_F0.5930.71
125_Y128_R0.5920.71
110_S141_I0.5910.71
68_E82_A0.5880.71
190_K205_K0.5870.70
113_L260_A0.5850.70
219_G251_F0.5840.70
269_N272_E0.5800.69
156_Y299_A0.5770.69
94_E98_A0.5760.69
184_S232_G0.5760.69
15_T56_L0.5760.69
44_K48_E0.5740.69
63_Q260_A0.5730.69
23_E44_K0.5730.69
156_Y295_E0.5690.68
69_Q102_K0.5690.68
106_T140_T0.5680.68
43_L124_S0.5670.68
168_M252_V0.5670.68
26_Y35_P0.5600.67
267_Y271_Y0.5580.67
238_L246_K0.5560.66
11_M110_S0.5540.66
24_R90_E0.5540.66
34_K123_Q0.5530.66
119_T137_G0.5530.66
161_V287_P0.5520.66
119_T189_I0.5520.66
151_P155_T0.5500.65
43_L46_R0.5500.65
92_F96_A0.5480.65
51_G58_Q0.5460.65
197_P202_E0.5450.65
286_R289_M0.5440.65
45_D272_E0.5430.64
129_A133_A0.5420.64
124_S138_G0.5400.64
42_D45_D0.5380.64
163_E295_E0.5380.64
151_P206_L0.5370.64
245_Q249_Q0.5360.63
177_M242_L0.5340.63
159_D293_K0.5340.63
190_K202_E0.5330.63
85_G125_Y0.5300.63
12_A114_A0.5300.63
18_K24_R0.5290.62
9_L93_I0.5290.62
265_V268_D0.5290.62
63_Q71_L0.5280.62
28_H94_E0.5270.62
162_K214_S0.5270.62
117_F123_Q0.5270.62
184_S234_D0.5260.62
250_A285_Y0.5250.62
70_H297_I0.5250.62
70_H294_P0.5230.62
11_M85_G0.5210.61
247_G283_S0.5200.61
237_D276_V0.5170.61
278_D283_S0.5160.61
162_K211_A0.5160.61
63_Q66_N0.5150.60
145_E307_K0.5150.60
295_E298_D0.5150.60
160_R287_P0.5140.60
271_Y276_V0.5120.60
179_I235_V0.5120.60
217_A246_K0.5080.59
21_D89_I0.5060.59
63_Q67_L0.5060.59
40_L44_K0.5040.59
165_Y169_P0.5030.59
202_E209_E0.5030.59
101_H139_L0.5000.58
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2h1vA 1 0.9935 100 0.19 Contact Map
1lbqA 2 0.9806 100 0.192 Contact Map
3hcnA 2 0.9903 100 0.202 Contact Map
2xwpA 5 0.7903 100 0.595 Contact Map
2xvyA 3 0.7677 100 0.645 Contact Map
4ccsA 1 0.729 99.8 0.794 Contact Map
2jh3A 2 0.7935 99.7 0.816 Contact Map
3lyhA 2 0.3935 99.7 0.82 Contact Map
1tjnA 4 0.3742 99.5 0.842 Contact Map
2xwsA 2 0.3774 99.4 0.846 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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