GREMLIN Database
SP5AF - Stage V sporulation protein AF
UniProt: P31845 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 493 (471)
Sequences: 2252 (1879)
Seq/√Len: 86.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
173_K203_E7.2001.00
245_A255_I7.0781.00
420_L432_G5.8131.00
179_E210_D4.7371.00
194_A199_V4.6021.00
111_V126_V4.5991.00
177_V206_I4.0831.00
375_Q379_E3.5251.00
226_V284_A3.4981.00
257_I264_V3.2551.00
310_F418_M3.0631.00
172_V186_S3.0431.00
236_V267_T3.0371.00
190_I193_I2.9971.00
106_V112_A2.9741.00
172_V188_C2.9411.00
187_I203_E2.9391.00
161_L257_I2.8701.00
148_D164_R2.8321.00
304_L433_F2.8141.00
340_I387_V2.7821.00
155_V158_T2.6941.00
98_T101_E2.6941.00
213_G250_L2.6781.00
112_A123_I2.6641.00
19_E47_Q2.6251.00
417_L432_G2.6241.00
180_R192_D2.6121.00
96_V123_I2.5171.00
441_A451_L2.4821.00
190_I249_V2.4131.00
196_P484_K2.4131.00
239_T245_A2.3871.00
113_V124_I2.3671.00
180_R485_N2.3631.00
161_L188_C2.3601.00
102_T112_A2.3271.00
169_R192_D2.2941.00
199_V254_V2.2721.00
95_K120_F2.2311.00
421_V429_L2.2121.00
201_I205_E2.2091.00
171_R191_E2.1681.00
417_L436_L2.1641.00
175_T207_A2.1601.00
137_P149_G2.1581.00
12_Y18_N2.1341.00
167_D180_R2.1201.00
344_D414_R2.1181.00
202_V267_T2.0991.00
31_S171_R2.0851.00
146_A282_R2.0681.00
13_R18_N2.0661.00
29_G171_R2.0121.00
275_V299_I1.9811.00
340_I418_M1.9791.00
202_V254_V1.9711.00
96_V101_E1.9081.00
205_E227_N1.9041.00
248_H266_I1.8961.00
177_V185_L1.8801.00
290_F412_M1.8571.00
211_V224_F1.8031.00
104_D182_K1.7991.00
52_N155_V1.7971.00
175_T203_E1.7951.00
34_V51_L1.7761.00
217_A261_S1.7491.00
427_K431_I1.7491.00
38_K45_E1.7391.00
136_E149_G1.7321.00
200_E204_K1.7291.00
114_I121_A1.7111.00
30_T173_K1.7051.00
165_I246_A1.6991.00
48_L113_V1.6971.00
307_P421_V1.6861.00
209_I221_V1.6781.00
85_E95_K1.6771.00
191_E196_P1.6711.00
201_I204_K1.6691.00
185_L258_V1.6671.00
154_I186_S1.6601.00
14_N17_K1.6571.00
188_C264_V1.6521.00
202_V256_I1.6441.00
337_I422_A1.6391.00
457_F462_L1.6351.00
16_A45_E1.6201.00
105_Q123_I1.6161.00
20_E24_N1.6001.00
281_Y464_Q1.5881.00
23_K36_V1.5851.00
110_L127_R1.5711.00
181_S213_G1.5601.00
298_G400_T1.5521.00
187_I254_V1.5511.00
105_Q110_L1.5401.00
30_T191_E1.5351.00
269_T274_H1.5311.00
298_G349_F1.5281.00
299_I410_N1.5231.00
175_T187_I1.5231.00
12_Y17_K1.4730.99
212_D477_R1.4670.99
218_D258_V1.4660.99
97_K120_F1.4580.99
102_T121_A1.4580.99
176_K184_D1.4530.99
196_P200_E1.4490.99
174_M184_D1.4490.99
92_Q125_D1.4400.99
453_P456_P1.4330.99
158_T172_V1.4260.99
306_L394_A1.4080.99
302_S401_T1.4060.99
293_W297_F1.4030.99
231_N235_L1.3940.99
311_L429_L1.3930.99
256_I267_T1.3830.99
19_E23_K1.3810.99
253_H273_H1.3760.99
34_V49_Y1.3700.99
439_A443_I1.3540.99
245_A264_V1.3250.99
289_T293_W1.3250.99
231_N439_A1.3210.99
347_I395_A1.3160.99
60_I111_V1.3150.99
94_E101_E1.3110.99
204_K208_S1.3040.99
53_G158_T1.3040.99
236_V265_I1.3040.99
176_K182_K1.3020.99
56_D128_S1.2990.99
36_V47_Q1.2960.99
19_E38_K1.2940.99
60_I126_V1.2920.99
15_P114_I1.2900.99
165_I250_L1.2730.98
203_E207_A1.2680.98
181_S250_L1.2670.98
53_G159_A1.2610.98
335_P338_L1.2500.98
15_P116_E1.2360.98
378_I389_L1.2300.98
55_C60_I1.2230.98
136_E147_R1.2160.98
346_G372_L1.2140.98
142_V277_H1.1940.98
193_I477_R1.1910.97
22_F34_V1.1860.97
150_F161_L1.1840.97
297_F345_L1.1730.97
134_P148_D1.1720.97
154_I257_I1.1690.97
63_L67_L1.1650.97
112_A121_A1.1550.97
348_E415_L1.1510.97
337_I419_I1.1400.97
255_I266_I1.1390.97
138_D147_R1.1370.97
344_D411_K1.1350.97
416_V420_L1.1340.97
36_V49_Y1.1230.96
148_D163_R1.1180.96
37_R93_V1.1100.96
477_R485_N1.1070.96
174_M186_S1.1070.96
169_R191_E1.1070.96
440_M451_L1.1060.96
194_A254_V1.1050.96
79_E82_D1.0960.96
235_L284_A1.0940.96
198_L254_V1.0940.96
304_L417_L1.0930.96
344_D391_V1.0930.96
266_I273_H1.0880.96
110_L125_D1.0820.96
58_A62_H1.0820.96
158_T188_C1.0800.96
274_H406_L1.0750.95
175_T206_I1.0740.95
108_S166_R1.0740.95
334_I338_L1.0730.95
245_A266_I1.0720.95
204_K207_A1.0670.95
255_I264_V1.0660.95
17_K20_E1.0650.95
141_K359_T1.0630.95
81_V89_L1.0620.95
328_L385_P1.0620.95
41_I46_I1.0570.95
53_G155_V1.0570.95
54_L159_A1.0560.95
201_I227_N1.0540.95
15_P47_Q1.0540.95
21_Y25_R1.0530.95
18_N114_I1.0470.95
129_Y153_N1.0460.95
148_D260_T1.0460.95
202_V446_L1.0440.95
195_D448_T1.0390.94
188_C249_V1.0340.94
165_I190_I1.0310.94
148_D160_L1.0300.94
254_V267_T1.0270.94
143_V279_E1.0230.94
40_T45_E1.0170.94
336_I387_V1.0150.94
233_F413_S1.0130.94
102_T114_I1.0110.94
341_F418_M1.0110.94
185_L256_I1.0090.94
215_T220_T1.0080.94
270_T273_H1.0070.93
347_I366_G1.0040.93
226_V235_L1.0000.93
30_T171_R0.9990.93
19_E49_Y0.9980.93
135_E149_G0.9950.93
418_M422_A0.9940.93
354_A362_S0.9920.93
47_Q114_I0.9900.93
344_D415_L0.9900.93
272_F454_L0.9860.93
180_R477_R0.9860.93
151_V157_N0.9840.93
275_V296_F0.9830.93
223_E468_R0.9820.93
251_E281_Y0.9820.93
307_P417_L0.9820.93
32_Y173_K0.9760.92
195_D482_H0.9650.92
192_D196_P0.9630.92
398_T407_S0.9570.92
397_G400_T0.9530.91
413_S436_L0.9530.91
272_F465_V0.9450.91
110_L123_I0.9450.91
271_L451_L0.9440.91
233_F439_A0.9440.91
183_T258_V0.9430.91
454_L466_L0.9420.91
180_R190_I0.9420.91
55_C126_V0.9350.91
129_Y163_R0.9340.91
292_R464_Q0.9330.91
38_K47_Q0.9300.90
164_R183_T0.9260.90
223_E471_K0.9200.90
159_A163_R0.9140.90
137_P240_E0.9110.90
140_E244_V0.9110.90
161_L245_A0.9060.89
344_D351_R0.9050.89
166_R184_D0.9020.89
96_V102_T0.9010.89
187_I202_V0.8990.89
62_H65_R0.8990.89
365_M395_A0.8990.89
310_F340_I0.8920.89
237_R273_H0.8920.89
180_R193_I0.8860.88
21_Y99_L0.8860.88
313_V336_I0.8850.88
200_E203_E0.8830.88
46_I113_V0.8800.88
271_L406_L0.8780.88
22_F49_Y0.8740.88
13_R119_G0.8740.88
233_F440_M0.8720.87
28_M34_V0.8700.87
49_Y114_I0.8700.87
33_D162_R0.8700.87
94_E105_Q0.8700.87
81_V85_E0.8690.87
290_F348_E0.8660.87
301_A393_L0.8630.87
469_T474_A0.8620.87
133_N151_V0.8600.87
247_N251_E0.8590.87
236_V446_L0.8580.87
351_R354_A0.8580.87
233_F436_L0.8560.86
35_G52_N0.8550.86
269_T273_H0.8550.86
357_T399_F0.8550.86
233_F450_Y0.8460.86
298_G397_G0.8400.85
155_V184_D0.8400.85
139_T263_S0.8380.85
205_E225_I0.8360.85
354_A363_T0.8350.85
328_L386_E0.8350.85
130_P159_A0.8340.85
270_T274_H0.8330.85
16_A20_E0.8320.85
81_V95_K0.8300.85
161_L170_L0.8270.84
199_V202_V0.8270.84
101_E104_D0.8270.84
25_R103_T0.8250.84
232_P439_A0.8230.84
104_D176_K0.8220.84
28_M31_S0.8210.84
131_G156_V0.8200.84
411_K415_L0.8170.84
164_R260_T0.8160.84
96_V112_A0.8150.84
303_T413_S0.8150.84
142_V278_A0.8140.84
209_I212_D0.8140.84
153_N184_D0.8110.83
84_V89_L0.8060.83
366_G386_E0.8050.83
337_I418_M0.8030.83
239_T255_I0.8030.83
359_T365_M0.8020.83
64_M68_V0.8000.82
237_R269_T0.7980.82
430_V434_T0.7970.82
483_P486_R0.7960.82
248_H273_H0.7940.82
417_L421_V0.7930.82
163_R259_D0.7920.82
244_V278_A0.7920.82
99_L114_I0.7910.82
226_V229_S0.7890.82
220_T247_N0.7860.81
180_R212_D0.7850.81
354_A364_A0.7850.81
419_I422_A0.7840.81
329_N332_T0.7830.81
49_Y106_V0.7820.81
132_R152_E0.7820.81
153_N156_V0.7800.81
139_T361_L0.7780.81
195_D483_P0.7750.80
364_A370_A0.7730.80
289_T469_T0.7720.80
193_I212_D0.7720.80
460_K476_V0.7710.80
97_K101_E0.7680.80
150_F245_A0.7670.80
53_G129_Y0.7670.80
217_A220_T0.7660.80
348_E351_R0.7610.79
441_A455_I0.7610.79
39_L64_M0.7590.79
28_M107_L0.7590.79
314_L423_L0.7560.79
271_L400_T0.7540.79
274_H411_K0.7520.78
452_W461_A0.7500.78
476_V487_L0.7480.78
193_I221_V0.7480.78
214_L221_V0.7460.78
417_L420_L0.7440.78
476_V479_S0.7440.78
460_K463_W0.7430.78
198_L446_L0.7400.77
28_M103_T0.7370.77
283_Q408_L0.7350.77
302_S410_N0.7340.77
391_V415_L0.7300.76
39_L46_I0.7290.76
108_S127_R0.7280.76
144_R149_G0.7270.76
237_R274_H0.7270.76
208_S444_K0.7250.76
343_A387_V0.7250.76
28_M171_R0.7220.76
346_G393_L0.7210.75
274_H369_A0.7180.75
32_Y158_T0.7180.75
102_T106_V0.7160.75
247_N276_Q0.7160.75
142_V279_E0.7160.75
167_D485_N0.7150.75
306_L344_D0.7150.75
63_L124_I0.7140.75
137_P263_S0.7090.74
379_E385_P0.7080.74
131_G153_N0.7070.74
160_L163_R0.7070.74
310_F314_L0.7070.74
415_L419_I0.7050.74
413_S416_V0.7010.73
21_Y24_N0.7010.73
187_I194_A0.6980.73
336_I366_G0.6970.73
46_I115_V0.6960.73
404_Y408_L0.6950.73
267_T446_L0.6950.73
237_R248_H0.6940.73
337_I341_F0.6930.73
59_Y126_V0.6930.73
158_T186_S0.6920.72
77_P80_L0.6920.72
234_P274_H0.6920.72
333_H352_M0.6900.72
166_R259_D0.6890.72
30_T203_E0.6870.72
245_A429_L0.6870.72
153_N259_D0.6860.72
463_W476_V0.6860.72
211_V214_L0.6850.72
148_D246_A0.6830.71
308_I454_L0.6810.71
219_K223_E0.6810.71
271_L299_I0.6810.71
39_L68_V0.6790.71
181_S215_T0.6770.71
22_F28_M0.6770.71
161_L246_A0.6770.71
368_I395_A0.6740.70
188_C257_I0.6730.70
458_N461_A0.6720.70
394_A418_M0.6720.70
274_H403_S0.6710.70
331_D392_S0.6700.70
452_W456_P0.6700.70
151_V156_V0.6680.70
307_P404_Y0.6670.70
328_L370_A0.6670.70
134_P160_L0.6660.70
144_R222_E0.6640.69
290_F345_L0.6630.69
308_I312_F0.6620.69
333_H376_I0.6600.69
51_L54_L0.6590.69
108_S184_D0.6580.69
334_I382_L0.6580.69
154_I259_D0.6570.68
127_R155_V0.6570.68
315_Q426_I0.6570.68
81_V122_F0.6570.68
463_W469_T0.6560.68
315_Q425_H0.6550.68
232_P287_V0.6550.68
215_T250_L0.6550.68
238_Y263_S0.6540.68
328_L384_S0.6540.68
241_R266_I0.6530.68
366_G444_K0.6530.68
128_S135_E0.6520.68
35_G50_Y0.6520.68
82_D86_N0.6500.68
329_N375_Q0.6500.68
278_A282_R0.6500.68
381_G456_P0.6490.68
140_E328_L0.6490.68
269_T401_T0.6490.68
173_K187_I0.6490.68
272_F296_F0.6470.67
275_V406_L0.6460.67
416_V436_L0.6440.67
38_K85_E0.6430.67
349_F366_G0.6430.67
104_D297_F0.6390.66
378_I385_P0.6380.66
219_K224_F0.6380.66
375_Q392_S0.6380.66
212_D478_P0.6370.66
237_R266_I0.6360.66
214_L220_T0.6360.66
197_D200_E0.6350.66
412_M485_N0.6340.66
97_K119_G0.6340.66
31_S34_V0.6330.66
270_T448_T0.6320.65
404_Y407_S0.6320.65
142_V146_A0.6320.65
68_V71_N0.6310.65
318_L404_Y0.6300.65
83_I88_L0.6300.65
276_Q292_R0.6290.65
15_P18_N0.6290.65
350_L368_I0.6280.65
130_P156_V0.6280.65
217_A282_R0.6270.65
140_E298_G0.6250.65
185_L221_V0.6240.65
336_I371_V0.6220.64
192_D484_K0.6220.64
225_I446_L0.6210.64
135_E138_D0.6210.64
198_L448_T0.6210.64
283_Q405_E0.6200.64
22_F36_V0.6200.64
92_Q165_I0.6200.64
448_T481_V0.6190.64
57_T153_N0.6190.64
340_I391_V0.6170.64
179_E485_N0.6150.63
232_P412_M0.6150.63
34_V190_I0.6150.63
164_R246_A0.6140.63
45_E116_E0.6130.63
22_F47_Q0.6110.63
235_L373_I0.6100.63
307_P407_S0.6080.63
301_A305_F0.6060.62
406_L409_A0.6060.62
475_K487_L0.6050.62
329_N384_S0.6040.62
481_V484_K0.6030.62
344_D418_M0.6030.62
270_T366_G0.6020.62
357_T370_A0.6000.61
464_Q474_A0.5970.61
53_G163_R0.5960.61
304_L429_L0.5950.61
418_M421_V0.5950.61
48_L124_I0.5940.61
196_P199_V0.5940.61
211_V218_D0.5920.60
305_F393_L0.5910.60
153_N163_R0.5900.60
481_V486_R0.5900.60
462_L465_V0.5900.60
227_N445_S0.5890.60
131_G151_V0.5880.60
355_I360_A0.5880.60
347_I354_A0.5880.60
173_K191_E0.5870.60
377_A383_F0.5870.60
175_T256_I0.5860.60
232_P344_D0.5860.60
54_L163_R0.5850.60
237_R244_V0.5850.60
352_M376_I0.5840.59
344_D407_S0.5830.59
429_L432_G0.5830.59
419_I423_L0.5830.59
437_I441_A0.5830.59
251_E276_Q0.5820.59
307_P344_D0.5820.59
137_P282_R0.5820.59
23_K49_Y0.5810.59
248_H253_H0.5810.59
397_G457_F0.5810.59
187_I199_V0.5810.59
316_P336_I0.5810.59
308_I426_I0.5810.59
352_M356_H0.5800.59
225_I236_V0.5800.59
357_T373_I0.5800.59
81_V120_F0.5790.59
48_L111_V0.5790.59
80_L84_V0.5780.59
300_L304_L0.5760.58
445_S449_P0.5760.58
218_D265_I0.5760.58
220_T251_E0.5750.58
83_I87_R0.5750.58
458_N479_S0.5750.58
276_Q281_Y0.5750.58
382_L389_L0.5750.58
464_Q468_R0.5740.58
310_F422_A0.5740.58
92_Q153_N0.5730.58
302_S398_T0.5730.58
95_K122_F0.5710.58
418_M424_F0.5710.58
357_T361_L0.5700.58
463_W474_A0.5690.57
321_D375_Q0.5690.57
302_S306_L0.5690.57
148_D183_T0.5680.57
324_K400_T0.5680.57
164_R241_R0.5670.57
332_T384_S0.5670.57
324_K386_E0.5670.57
201_I225_I0.5670.57
229_S472_P0.5660.57
255_I267_T0.5660.57
180_R250_L0.5650.57
228_Q235_L0.5630.57
350_L388_I0.5620.57
366_G371_V0.5620.57
306_L390_Y0.5620.57
193_I485_N0.5620.57
431_I435_V0.5610.56
160_L164_R0.5610.56
214_L258_V0.5600.56
280_E352_M0.5600.56
365_M389_L0.5590.56
195_D481_V0.5590.56
202_V227_N0.5580.56
81_V84_V0.5560.56
169_R180_R0.5560.56
14_N19_E0.5550.56
211_V471_K0.5550.56
417_L429_L0.5540.55
285_P370_A0.5540.55
93_V122_F0.5540.55
333_H425_H0.5530.55
281_Y463_W0.5530.55
311_L421_V0.5510.55
269_T272_F0.5510.55
149_G263_S0.5500.55
269_T346_G0.5500.55
37_R151_V0.5500.55
205_E445_S0.5490.55
294_V372_L0.5480.55
16_A38_K0.5480.55
209_I224_F0.5470.55
468_R471_K0.5470.55
147_R263_S0.5470.55
115_V476_V0.5470.55
372_L440_M0.5470.55
319_L322_N0.5470.55
253_H276_Q0.5460.54
339_Q352_M0.5460.54
101_E105_Q0.5450.54
161_L361_L0.5450.54
52_N92_Q0.5450.54
170_L249_V0.5450.54
239_T402_P0.5450.54
194_A198_L0.5450.54
353_A395_A0.5450.54
417_L433_F0.5440.54
22_F114_I0.5440.54
161_L164_R0.5440.54
319_L360_A0.5440.54
454_L465_V0.5440.54
64_M251_E0.5420.54
151_V184_D0.5420.54
314_L425_H0.5420.54
201_I208_S0.5420.54
299_I401_T0.5400.54
32_Y174_M0.5400.54
251_E464_Q0.5400.54
154_I184_D0.5390.53
274_H344_D0.5390.53
236_V371_V0.5370.53
354_A360_A0.5370.53
468_R474_A0.5360.53
192_D318_L0.5350.53
150_F264_V0.5340.53
210_D477_R0.5340.53
20_E23_K0.5340.53
52_N57_T0.5340.53
135_E423_L0.5340.53
97_K100_D0.5330.53
146_A405_E0.5330.53
230_Y473_G0.5320.53
361_L365_M0.5320.53
220_T471_K0.5310.52
84_V87_R0.5310.52
177_V199_V0.5310.52
449_P456_P0.5300.52
357_T382_L0.5290.52
151_V301_A0.5280.52
251_E254_V0.5270.52
298_G364_A0.5270.52
202_V355_I0.5260.52
273_H276_Q0.5240.51
105_Q177_V0.5240.51
459_G463_W0.5230.51
242_P246_A0.5220.51
318_L395_A0.5210.51
407_S411_K0.5210.51
198_L482_H0.5210.51
347_I391_V0.5200.51
100_D182_K0.5200.51
108_S206_I0.5190.51
15_P102_T0.5190.51
356_H376_I0.5180.51
181_S366_G0.5170.50
222_E238_Y0.5170.50
384_S425_H0.5160.50
304_L414_R0.5160.50
179_E196_P0.5150.50
190_I206_I0.5150.50
229_S284_A0.5140.50
25_R99_L0.5130.50
217_A221_V0.5120.50
391_V398_T0.5120.50
360_A366_G0.5120.50
114_I122_F0.5110.50
232_P241_R0.5110.50
299_I370_A0.5100.50
28_M167_D0.5100.50
144_R218_D0.5100.50
193_I199_V0.5100.50
303_T410_N0.5090.49
198_L201_I0.5080.49
273_H354_A0.5080.49
351_R413_S0.5070.49
55_C196_P0.5070.49
88_L124_I0.5070.49
222_E265_I0.5070.49
129_Y155_V0.5060.49
343_A365_M0.5060.49
24_N29_G0.5050.49
195_D199_V0.5040.49
185_L206_I0.5040.49
315_Q367_L0.5020.48
391_V418_M0.5020.48
150_F157_N0.5020.48
332_T389_L0.5020.48
183_T213_G0.5010.48
298_G480_I0.5000.48
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3hh1A 2 0.213 24 0.982 Contact Map
1s94A 1 0.2211 15.2 0.983 Contact Map
4bemA 3 0.1643 14.7 0.984 Contact Map
3kpeA 3 0.0994 14.5 0.984 Contact Map
2xndJ 3 0.146 9.8 0.985 Contact Map
2xquA 3 0.1643 9.6 0.985 Contact Map
4v1gA 3 0.1724 9.4 0.985 Contact Map
1ez3A 1 0.2231 8.2 0.985 Contact Map
3zk1A 5 0.1805 7.6 0.985 Contact Map
3h0lB 1 0.2434 6.6 0.986 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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