HEM2 - BACSU - GREMLIN Contacts

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HEM2 - Delta-aminolevulinic acid dehydratase

UniProt:	P30950	Sequences: MSQSFNRHRRLRTSKAMREMVKETRLHPSDFIYPIFVVEGLEGKKAVPSMPDVHHVSLDLLKDEVAELVKLGIQSVIVFGIPEEKDDCGTQAYHDHGIVQKAITEIKEHFPEMVVVADTCLCEYTDHGHCGLVKDGVILNDESLELLAQTAVSQAKAGADIIAPSNMMDGFVTVIREALDKEGFVNIPIMSYAVKYSSEFYGPFRDAANSTPQFGDRKTYQMDPANRMEALREAQSDVEEGADFLIVKPSLSYMDIMRDVKNEFTLPLVAYNVSGEYSMVKAAAQNGWIKEKEIVLEILTSMKRAGADLIITYHAKDAAKWLAE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	324 (318)
Sequences:	2874 (1470)
Seq/√Len:	82.4

ECOLI - HEM2
BACSU - HEM2
HALSA - Q9HMZ1
SULSO - Q980U6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
227_R	259_D	3.498	1.00
258_R	262_N	3.483	1.00
234_A	260_V	3.476	1.00
91_Q	94_H	3.434	1.00
87_D	126_D	3.087	1.00
64_E	316_K	3.042	1.00
30_D	303_K	3.007	1.00
292_K	321_W	2.909	1.00
117_A	154_Q	2.803	1.00
166_N	171_F	2.623	1.00
250_S	302_M	2.594	1.00
238_V	266_L	2.581	1.00
299_L	318_A	2.501	1.00
100_Q	153_S	2.479	1.00
234_A	245_L	2.473	1.00
103_I	115_V	2.424	1.00
156_K	184_F	2.325	1.00
152_V	178_A	2.297	1.00
107_K	160_D	2.291	1.00
137_V	186_N	2.216	1.00
22_K	223_D	2.212	1.00
281_K	291_E	2.188	1.00
227_R	256_I	2.168	1.00
44_K	54_H	2.140	1.00
139_L	142_E	2.116	1.00
93_Y	146_L	2.044	1.00
261_K	308_D	2.042	1.00
290_K	293_E	2.035	1.00
46_A	54_H	2.034	1.00
66_A	70_K	2.031	1.00
67_E	320_K	2.020	1.00
295_V	321_W	2.014	1.00
194_V	234_A	1.998	1.00
257_M	270_A	1.998	1.00
95_D	156_K	1.952	1.00
282_A	288_W	1.948	1.00
269_V	309_L	1.922	1.00
8_H	236_S	1.919	1.00
15_K	19_E	1.908	1.00
45_K	55_H	1.873	1.00
244_F	269_V	1.862	1.00
148_A	178_A	1.829	1.00
69_V	110_F	1.820	1.00
86_D	90_T	1.811	1.00
103_I	117_A	1.800	1.00
62_K	110_F	1.759	1.00
32_I	309_L	1.756	1.00
235_Q	239_E	1.732	1.00
60_L	63_D	1.732	1.00
228_M	261_K	1.729	1.00
259_D	262_N	1.686	1.00
44_K	60_L	1.686	1.00
93_Y	149_Q	1.674	1.00
19_E	74_Q	1.645	1.00
296_L	318_A	1.641	1.00
48_P	206_D	1.639	1.00
6_N	232_R	1.622	1.00
62_K	109_H	1.596	1.00
58_L	105_E	1.595	1.00
47_V	53_V	1.589	1.00
235_Q	264_F	1.588	1.00
132_L	146_L	1.586	1.00
251_L	272_N	1.569	1.00
63_D	66_A	1.567	1.00
75_S	116_V	1.554	1.00
200_F	298_I	1.552	1.00
61_L	106_I	1.538	1.00
295_V	314_H	1.512	0.99
44_K	56_V	1.495	0.99
66_A	110_F	1.486	0.99
43_G	57_S	1.475	0.99
19_E	29_S	1.475	0.99
228_M	259_D	1.472	0.99
252_S	301_S	1.465	0.99
25_R	29_S	1.462	0.99
161_I	244_F	1.459	0.99
76_V	115_V	1.438	0.99
42_E	60_L	1.437	0.99
39_E	101_K	1.433	0.99
317_D	320_K	1.431	0.99
254_M	302_M	1.431	0.99
76_V	113_M	1.431	0.99
156_K	182_E	1.424	0.99
67_E	316_K	1.407	0.99
33_Y	68_L	1.399	0.99
121_L	150_T	1.392	0.99
250_S	270_A	1.391	0.99
49_S	206_D	1.390	0.99
291_E	295_V	1.379	0.99
190_M	244_F	1.376	0.99
260_V	268_L	1.372	0.99
272_N	314_H	1.361	0.99
77_I	311_I	1.356	0.99
77_I	163_A	1.349	0.99
38_V	45_K	1.348	0.99
117_A	159_A	1.330	0.99
291_E	317_D	1.327	0.99
280_V	298_I	1.327	0.99
134_K	142_E	1.325	0.99
11_L	21_V	1.318	0.98
238_V	245_L	1.317	0.98
31_F	318_A	1.314	0.98
119_T	162_I	1.312	0.98
65_V	110_F	1.296	0.98
62_K	105_E	1.284	0.98
289_I	294_I	1.265	0.98
193_A	234_A	1.265	0.98
238_V	264_F	1.264	0.98
65_V	106_I	1.261	0.98
24_T	302_M	1.256	0.98
161_I	188_P	1.256	0.98
65_V	113_M	1.252	0.98
152_V	182_E	1.246	0.98
200_F	252_S	1.245	0.98
272_N	298_I	1.235	0.98
148_A	174_V	1.230	0.98
32_I	116_V	1.220	0.97
40_G	59_D	1.216	0.97
97_G	100_Q	1.212	0.97
114_V	161_I	1.210	0.97
281_K	317_D	1.205	0.97
65_V	76_V	1.205	0.97
64_E	68_L	1.205	0.97
235_Q	263_E	1.203	0.97
22_K	225_A	1.193	0.97
56_V	60_L	1.187	0.97
199_E	293_E	1.186	0.97
66_A	109_H	1.181	0.97
270_A	302_M	1.176	0.97
228_M	308_D	1.169	0.97
69_V	113_M	1.149	0.96
252_S	298_I	1.138	0.96
245_L	266_L	1.117	0.96
33_Y	53_V	1.116	0.96
251_L	298_I	1.113	0.96
284_A	289_I	1.109	0.96
178_A	182_E	1.109	0.96
105_E	108_E	1.099	0.95
134_K	139_L	1.098	0.95
312_T	315_A	1.096	0.95
251_L	301_S	1.090	0.95
199_E	294_I	1.090	0.95
89_G	125_T	1.088	0.95
299_L	302_M	1.087	0.95
318_A	321_W	1.074	0.95
96_H	101_K	1.072	0.95
16_A	114_V	1.068	0.95
68_L	316_K	1.067	0.95
284_A	295_V	1.063	0.94
127_H	133_V	1.061	0.94
85_K	211_T	1.061	0.94
200_F	280_V	1.059	0.94
13_T	186_N	1.046	0.94
245_L	260_V	1.044	0.94
38_V	43_G	1.040	0.94
227_R	258_R	1.032	0.93
196_Y	222_M	1.031	0.93
292_K	295_V	1.023	0.93
120_C	130_C	1.017	0.93
13_T	137_V	1.016	0.93
62_K	66_A	0.997	0.92
17_M	114_V	0.993	0.92
31_F	312_T	0.992	0.92
107_K	115_V	0.991	0.92
14_S	188_P	0.990	0.92
225_A	300_T	0.983	0.92
116_V	163_A	0.981	0.92
55_H	209_N	0.973	0.91
106_I	110_F	0.964	0.91
303_K	310_I	0.963	0.91
73_I	319_A	0.961	0.91
147_L	166_N	0.961	0.91
261_K	265_T	0.960	0.91
37_V	61_L	0.955	0.91
38_V	55_H	0.954	0.90
119_T	154_Q	0.952	0.90
280_V	314_H	0.951	0.90
254_M	257_M	0.948	0.90
69_V	112_E	0.947	0.90
245_L	268_L	0.944	0.90
33_Y	316_K	0.944	0.90
151_A	162_I	0.941	0.90
50_M	278_S	0.940	0.90
28_P	319_A	0.940	0.90
298_I	314_H	0.939	0.90
231_L	234_A	0.939	0.90
71_L	320_K	0.936	0.90
298_I	301_S	0.934	0.90
38_V	57_S	0.934	0.90
172_V	189_I	0.932	0.89
13_T	17_M	0.931	0.89
296_L	321_W	0.930	0.89
86_D	89_G	0.929	0.89
281_K	285_Q	0.928	0.89
71_L	316_K	0.922	0.89
58_L	102_A	0.921	0.89
116_V	161_I	0.914	0.88
173_T	177_E	0.911	0.88
103_I	159_A	0.909	0.88
235_Q	265_T	0.905	0.88
244_F	309_L	0.905	0.88
93_Y	153_S	0.900	0.88
68_L	76_V	0.899	0.88
64_E	67_E	0.897	0.88
120_C	217_R	0.896	0.87
49_S	288_W	0.896	0.87
257_M	302_M	0.894	0.87
230_A	256_I	0.892	0.87
123_E	209_N	0.891	0.87
280_V	289_I	0.889	0.87
105_E	109_H	0.889	0.87
17_M	188_P	0.887	0.87
130_C	217_R	0.884	0.87
42_E	59_D	0.882	0.87
5_F	8_H	0.873	0.86
280_V	294_I	0.872	0.86
292_K	296_L	0.869	0.86
99_V	124_Y	0.868	0.86
164_P	175_I	0.867	0.86
121_L	146_L	0.865	0.86
88_C	93_Y	0.862	0.85
200_F	294_I	0.860	0.85
146_L	150_T	0.857	0.85
41_L	57_S	0.854	0.85
6_N	239_E	0.853	0.85
254_M	301_S	0.850	0.85
291_E	321_W	0.846	0.84
35_I	61_L	0.844	0.84
190_M	309_L	0.842	0.84
106_I	113_M	0.841	0.84
194_V	245_L	0.841	0.84
28_P	31_F	0.832	0.83
296_L	300_T	0.832	0.83
284_A	291_E	0.829	0.83
257_M	307_A	0.829	0.83
32_I	311_I	0.823	0.83
281_K	295_V	0.822	0.83
145_E	149_Q	0.820	0.82
231_L	260_V	0.820	0.82
245_L	264_F	0.817	0.82
146_L	149_Q	0.812	0.82
154_Q	162_I	0.810	0.82
281_K	314_H	0.806	0.81
144_L	174_V	0.803	0.81
318_A	322_L	0.803	0.81
314_H	321_W	0.801	0.81
104_T	156_K	0.795	0.80
14_S	17_M	0.793	0.80
280_V	284_A	0.788	0.80
282_A	286_N	0.785	0.80
5_F	177_E	0.784	0.79
103_I	154_Q	0.784	0.79
171_F	191_S	0.782	0.79
66_A	69_V	0.781	0.79
39_E	83_E	0.780	0.79
200_F	251_L	0.777	0.79
119_T	166_N	0.776	0.79
265_T	268_L	0.774	0.79
151_A	175_I	0.772	0.78
26_L	31_F	0.768	0.78
65_V	105_E	0.764	0.78
35_I	78_V	0.763	0.78
58_L	98_I	0.762	0.78
106_I	115_V	0.756	0.77
35_I	64_E	0.756	0.77
272_N	280_V	0.754	0.77
125_T	133_V	0.754	0.77
38_V	41_L	0.746	0.76
133_V	138_I	0.746	0.76
104_T	108_E	0.738	0.75
80_G	130_C	0.738	0.75
230_A	234_A	0.737	0.75
231_L	235_Q	0.736	0.75
87_D	127_H	0.735	0.75
20_M	74_Q	0.734	0.75
246_I	311_I	0.731	0.75
53_V	277_Y	0.731	0.75
296_L	322_L	0.729	0.74
315_A	319_A	0.729	0.74
26_L	299_L	0.727	0.74
80_G	120_C	0.726	0.74
56_V	64_E	0.725	0.74
26_L	318_A	0.721	0.74
194_V	260_V	0.720	0.73
226_N	229_E	0.718	0.73
200_F	314_H	0.716	0.73
26_L	296_L	0.715	0.73
120_C	123_E	0.715	0.73
80_G	217_R	0.714	0.73
47_V	206_D	0.714	0.73
14_S	114_V	0.714	0.73
58_L	62_K	0.712	0.73
45_K	209_N	0.707	0.72
6_N	236_S	0.707	0.72
61_L	65_V	0.706	0.72
81_I	123_E	0.705	0.72
43_G	60_L	0.702	0.72
81_I	209_N	0.701	0.71
76_V	106_I	0.701	0.71
191_S	194_V	0.701	0.71
122_C	130_C	0.699	0.71
147_L	171_F	0.697	0.71
123_E	130_C	0.697	0.71
82_P	98_I	0.696	0.71
119_T	171_F	0.694	0.71
252_S	302_M	0.694	0.71
208_A	212_P	0.688	0.70
40_G	43_G	0.687	0.70
231_L	263_E	0.687	0.70
152_V	155_A	0.680	0.69
279_M	283_A	0.678	0.69
37_V	102_A	0.673	0.68
5_F	236_S	0.672	0.68
291_E	314_H	0.671	0.68
138_I	168_M	0.669	0.68
78_V	103_I	0.665	0.67
155_A	187_I	0.658	0.67
269_V	311_I	0.656	0.66
294_I	298_I	0.655	0.66
123_E	217_R	0.653	0.66
246_I	269_V	0.653	0.66
71_L	319_A	0.651	0.66
55_H	123_E	0.650	0.66
252_S	272_N	0.646	0.65
57_S	60_L	0.643	0.65
58_L	101_K	0.642	0.65
8_H	11_L	0.639	0.64
19_E	225_A	0.638	0.64
224_P	300_T	0.638	0.64
21_V	223_D	0.634	0.64
295_V	317_D	0.633	0.64
251_L	302_M	0.632	0.64
228_M	258_R	0.631	0.63
82_P	100_Q	0.631	0.63
65_V	68_L	0.630	0.63
44_K	64_E	0.630	0.63
122_C	208_A	0.626	0.63
178_A	181_K	0.625	0.63
62_K	65_V	0.625	0.63
51_P	288_W	0.625	0.63
281_K	284_A	0.624	0.63
143_S	147_L	0.624	0.63
36_F	204_F	0.623	0.62
272_N	299_L	0.622	0.62
218_K	293_E	0.621	0.62
41_L	59_D	0.621	0.62
133_V	139_L	0.620	0.62
73_I	316_K	0.618	0.62
122_C	217_R	0.616	0.62
23_E	226_N	0.615	0.61
77_I	116_V	0.612	0.61
155_A	188_P	0.611	0.61
197_S	218_K	0.610	0.61
137_V	185_V	0.608	0.61
261_K	266_L	0.606	0.60
49_S	278_S	0.605	0.60
100_Q	154_Q	0.605	0.60
11_L	188_P	0.605	0.60
121_L	132_L	0.604	0.60
284_A	290_K	0.601	0.60
15_K	22_K	0.601	0.60
137_V	219_T	0.600	0.60
234_A	238_V	0.599	0.59
130_C	208_A	0.599	0.59
35_I	76_V	0.597	0.59
295_V	298_I	0.594	0.59
71_L	74_Q	0.593	0.59
172_V	175_I	0.592	0.59
100_Q	150_T	0.592	0.59
16_A	19_E	0.591	0.58
40_G	57_S	0.590	0.58
180_D	185_V	0.590	0.58
142_E	145_E	0.587	0.58
43_G	59_D	0.586	0.58
227_R	262_N	0.585	0.58
17_M	20_M	0.585	0.58
68_L	113_M	0.583	0.57
121_L	171_F	0.582	0.57
24_T	300_T	0.580	0.57
224_P	253_Y	0.580	0.57
52_D	317_D	0.579	0.57
77_I	80_G	0.579	0.57
81_I	85_K	0.578	0.57
69_V	111_P	0.578	0.57
93_Y	150_T	0.578	0.57
120_C	208_A	0.577	0.57
8_H	232_R	0.577	0.57
25_R	30_D	0.577	0.57
132_L	150_T	0.576	0.56
228_M	265_T	0.576	0.56
261_K	264_F	0.575	0.56
121_L	166_N	0.575	0.56
259_D	263_E	0.574	0.56
61_L	102_A	0.573	0.56
162_I	189_I	0.573	0.56
196_Y	227_R	0.571	0.56
56_V	65_V	0.571	0.56
298_I	302_M	0.570	0.56
84_E	91_Q	0.569	0.56
24_T	225_A	0.568	0.55
312_T	318_A	0.567	0.55
250_S	257_M	0.564	0.55
176_R	180_D	0.564	0.55
162_I	175_I	0.563	0.55
38_V	59_D	0.563	0.55
45_K	123_E	0.561	0.54
89_G	121_L	0.561	0.54
107_K	111_P	0.561	0.54
96_H	104_T	0.559	0.54
174_V	178_A	0.558	0.54
31_F	116_V	0.557	0.54
80_G	123_E	0.556	0.54
193_A	260_V	0.554	0.54
65_V	109_H	0.554	0.54
17_M	161_I	0.553	0.53
261_K	268_L	0.553	0.53
138_I	220_Y	0.553	0.53
32_I	75_S	0.552	0.53
24_T	227_R	0.550	0.53
91_Q	150_T	0.550	0.53
161_I	190_M	0.549	0.53
110_F	113_M	0.548	0.53
47_V	55_H	0.545	0.52
36_F	271_Y	0.545	0.52
92_A	150_T	0.545	0.52
78_V	99_V	0.544	0.52
81_I	120_C	0.539	0.52
30_D	310_I	0.537	0.51
154_Q	159_A	0.532	0.51
216_D	293_E	0.532	0.51
63_D	109_H	0.530	0.50
81_I	130_C	0.528	0.50
50_M	288_W	0.528	0.50
208_A	217_R	0.528	0.50
314_H	317_D	0.528	0.50
260_V	263_E	0.527	0.50
91_Q	123_E	0.526	0.50
81_I	217_R	0.526	0.50
143_S	166_N	0.525	0.50
280_V	291_E	0.524	0.50
42_E	292_K	0.524	0.50
228_M	232_R	0.522	0.49
302_M	307_A	0.522	0.49
192_Y	210_S	0.521	0.49
33_Y	64_E	0.521	0.49
117_A	151_A	0.520	0.49
41_L	101_K	0.520	0.49
11_L	17_M	0.520	0.49
63_D	67_E	0.519	0.49
142_E	146_L	0.519	0.49
66_A	108_E	0.519	0.49
138_I	219_T	0.518	0.49
278_S	282_A	0.516	0.49
258_R	261_K	0.514	0.48
7_R	10_R	0.513	0.48
283_A	288_W	0.512	0.48
35_I	316_K	0.512	0.48
270_A	307_A	0.511	0.48
67_E	71_L	0.510	0.48
41_L	44_K	0.509	0.48
77_I	122_C	0.509	0.48
11_L	14_S	0.508	0.48
38_V	60_L	0.508	0.48
66_A	112_E	0.508	0.48
133_V	213_Q	0.507	0.47
46_A	285_Q	0.507	0.47
66_A	156_K	0.505	0.47
73_I	113_M	0.505	0.47
26_L	300_T	0.504	0.47
133_V	137_V	0.503	0.47
78_V	106_I	0.503	0.47
42_E	57_S	0.503	0.47
134_K	137_V	0.502	0.47
47_V	207_A	0.502	0.47
35_I	65_V	0.501	0.47
132_L	142_E	0.500	0.46
235_Q	238_V	0.500	0.46

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1w1zA	5	0.9846	100	-0.011	Contact Map
1w5qA	5	0.9722	100	-0.002	Contact Map
3obkA	5	0.9969	100	0.005	Contact Map
1l6sA	5	0.9938	100	0.017	Contact Map
1h7nA	5	1	100	0.037	Contact Map
1pv8A	5	0.8642	100	0.044	Contact Map
3vcrA	3	0.5988	97.2	0.949	Contact Map
1wbhA	3	0.5833	97.2	0.949	Contact Map
3lg3A	4	0.8148	96.9	0.952	Contact Map
3i4eA	4	0.8302	96.7	0.953	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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