GREMLIN Database
RF2 - Peptide chain release factor 2
UniProt: P28367 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 366 (336)
Sequences: 5925 (3041)
Seq/√Len: 165.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
298_R301_E4.4931.00
296_Q300_E3.2571.00
300_E304_A3.0941.00
129_L180_L3.0601.00
32_R58_E3.0291.00
158_R354_D3.0271.00
297_R301_E2.8051.00
327_F349_M2.7811.00
30_E70_K2.7701.00
243_Y272_T2.7511.00
77_E81_M2.6391.00
155_G182_I2.6251.00
240_V262_I2.5681.00
126_N224_E2.5511.00
152_T162_V2.5331.00
244_R257_D2.5101.00
48_D51_K2.4971.00
238_I262_I2.4871.00
344_N347_A2.4581.00
238_I287_M2.3881.00
148_L180_L2.3691.00
71_K108_E2.3491.00
36_L55_V2.3001.00
262_I287_M2.2161.00
301_E305_E2.1931.00
239_K265_L2.1911.00
148_L178_V2.1531.00
44_E47_N2.1301.00
280_I284_E2.1271.00
158_R190_Y2.1051.00
348_V356_F2.0921.00
51_K54_T2.0331.00
35_E39_Q2.0311.00
248_A252_H2.0241.00
245_A252_H2.0101.00
135_A216_F2.0041.00
78_E81_M1.9771.00
63_K67_N1.9511.00
163_E183_K1.9381.00
347_A352_D1.9191.00
299_I303_Q1.8871.00
39_Q51_K1.8741.00
131_L144_G1.8321.00
248_A277_R1.8291.00
148_L152_T1.8051.00
79_L102_L1.7931.00
207_D211_R1.7881.00
74_E78_E1.7761.00
147_L217_V1.7731.00
252_H256_T1.7691.00
108_E111_E1.7411.00
252_H277_R1.7321.00
154_W354_D1.7051.00
241_D263_T1.6971.00
295_Y299_I1.6901.00
340_T355_T1.6651.00
83_H106_T1.6561.00
37_D63_K1.6501.00
252_H258_S1.6461.00
104_S108_E1.6441.00
303_Q307_D1.6421.00
163_E181_L1.6251.00
299_I302_Q1.6191.00
276_E281_K1.6151.00
126_N183_K1.6131.00
207_D213_H1.6051.00
162_V180_L1.6011.00
40_M56_I1.6001.00
59_A63_K1.5991.00
39_Q42_D1.5881.00
167_Y175_I1.5521.00
252_H255_T1.5421.00
34_A37_D1.5411.00
37_D59_A1.5241.00
276_E285_R1.5071.00
148_L164_T1.5031.00
263_T270_V1.4991.00
333_V356_F1.4831.00
240_V283_R1.4831.00
327_F345_V1.4601.00
298_R302_Q1.4491.00
242_T260_V1.4131.00
228_E291_K1.4131.00
245_A255_T1.4121.00
69_Y73_N1.4051.00
149_R350_D1.4021.00
275_T285_R1.4001.00
75_S108_E1.3891.00
242_T257_D1.3861.00
284_E288_K1.3851.00
161_K183_K1.3851.00
302_Q306_L1.3661.00
50_Q54_T1.3551.00
289_M293_K1.3551.00
164_T178_V1.3401.00
50_Q53_Q1.3341.00
305_E308_E1.3331.00
239_K263_T1.3321.00
107_K111_E1.3271.00
285_R288_K1.3181.00
100_K104_S1.3091.00
157_R352_D1.3051.00
68_S115_Q1.3021.00
131_L178_V1.2781.00
238_I265_L1.2761.00
155_G162_V1.2731.00
96_L100_K1.2561.00
345_V349_M1.2531.00
280_I283_R1.2531.00
160_F191_L1.2461.00
229_I295_Y1.2451.00
342_M348_V1.2431.00
52_A56_I1.2361.00
331_S346_Q1.2341.00
97_E101_E1.2321.00
216_F316_I1.2181.00
203_I210_G1.2131.00
40_M45_F1.2071.00
300_E303_Q1.2021.00
67_N71_K1.2001.00
27_E31_A1.1941.00
269_V297_R1.1891.00
264_H268_N1.1861.00
138_T170_G1.1841.00
32_R35_E1.1791.00
33_I59_A1.1781.00
324_S336_H1.1751.00
75_S101_E1.1701.00
124_K186_N1.1661.00
348_V353_I1.1631.00
131_L219_C1.1521.00
314_K320_S1.1481.00
181_L224_E1.1441.00
259_A274_Q1.1391.00
264_H267_T1.1371.00
106_T110_N1.1101.00
153_R350_D1.1081.00
333_V348_V1.0981.00
281_K288_K1.0921.00
152_T180_L1.0921.00
68_S71_K1.0921.00
342_M355_T1.0911.00
207_D210_G1.0881.00
128_I181_L1.0851.00
303_Q306_L1.0851.00
95_Q99_E1.0821.00
128_I165_L1.0680.99
99_E103_K1.0670.99
42_D45_F1.0650.99
190_Y358_D1.0640.99
182_I191_L1.0590.99
304_A308_E1.0570.99
235_T238_I1.0550.99
252_H257_D1.0500.99
243_Y261_R1.0490.99
127_A189_G1.0480.99
157_R350_D1.0480.99
49_Q52_A1.0480.99
71_K75_S1.0470.99
75_S109_F1.0420.99
122_Y125_N1.0370.99
81_M84_D1.0330.99
307_D311_G1.0320.99
96_L99_E1.0310.99
73_N77_E1.0310.99
242_T252_H1.0270.99
342_M345_V1.0250.99
175_I178_V1.0220.99
34_A38_E1.0220.99
70_K73_N1.0180.99
142_D172_E1.0140.99
129_L182_I1.0110.99
190_Y354_D1.0090.99
272_T275_T1.0020.99
344_N355_T1.0020.99
340_T359_A1.0000.99
233_I287_M0.9970.99
72_L76_H0.9950.99
49_Q53_Q0.9930.99
32_R39_Q0.9910.99
146_M327_F0.9820.99
355_T359_A0.9790.99
94_L98_L0.9780.99
100_K103_K0.9760.99
220_E310_R0.9720.99
302_Q305_E0.9670.99
154_W190_Y0.9570.99
195_K220_E0.9560.99
301_E304_A0.9520.99
124_K185_H0.9440.99
144_G217_V0.9410.99
241_D261_R0.9380.99
80_Q84_D0.9350.99
46_W52_A0.9280.99
61_G185_H0.9270.99
78_E105_L0.9260.99
148_L175_I0.9250.99
160_F187_A0.9210.98
122_Y299_I0.9200.98
30_E73_N0.9190.98
193_A360_Y0.9180.98
66_V70_K0.9180.98
164_T167_Y0.9160.98
114_L117_L0.9150.98
46_W49_Q0.9080.98
296_Q299_I0.9070.98
67_N70_K0.9070.98
33_I66_V0.9060.98
275_T289_M0.9060.98
28_S31_A0.9060.98
30_E34_A0.8990.98
269_V294_L0.8980.98
252_H280_I0.8970.98
31_A35_E0.8970.98
238_I266_P0.8890.98
353_I357_I0.8880.98
126_N181_L0.8880.98
331_S345_V0.8860.98
275_T281_K0.8840.98
354_D358_D0.8800.98
64_D67_N0.8760.98
141_Q172_E0.8750.98
352_D355_T0.8740.98
190_Y361_L0.8710.98
271_V294_L0.8650.98
265_L268_N0.8590.98
153_R156_E0.8590.98
33_I63_K0.8580.98
272_T289_M0.8530.97
353_I356_F0.8510.97
235_T287_M0.8500.97
93_D97_E0.8490.97
310_R313_Q0.8400.97
154_W160_F0.8380.97
27_E73_N0.8380.97
255_T260_V0.8340.97
283_R287_M0.8320.97
81_M85_L0.8310.97
242_T248_A0.8300.97
262_I283_R0.8300.97
201_V324_S0.8300.97
129_L191_L0.8270.97
133_P140_S0.8200.97
165_L181_L0.8170.97
291_K295_Y0.8160.97
38_E41_A0.8130.97
277_R288_K0.8080.97
145_S170_G0.8070.96
324_S332_M0.8060.96
33_I62_L0.8040.96
79_L105_L0.8030.96
340_T348_V0.8030.96
133_P168_L0.8010.96
326_V332_M0.8010.96
103_K106_T0.7930.96
40_M59_A0.7890.96
181_L288_K0.7890.96
39_Q55_V0.7890.96
167_Y178_V0.7870.96
230_D291_K0.7870.96
281_K284_E0.7840.96
201_V326_V0.7830.96
297_R300_E0.7790.96
76_H80_Q0.7780.96
123_D192_K0.7770.96
33_I37_D0.7760.96
357_I361_L0.7760.96
88_E91_D0.7710.95
203_I209_S0.7710.95
225_F230_D0.7710.95
334_K341_E0.7700.95
122_Y295_Y0.7690.95
145_S175_I0.7610.95
229_I291_K0.7610.95
110_N113_E0.7600.95
97_E100_K0.7580.95
72_L75_S0.7560.95
102_L106_T0.7560.95
103_K107_K0.7470.95
105_L109_F0.7450.95
248_A255_T0.7450.95
241_D265_L0.7420.94
141_Q145_S0.7410.94
294_L297_R0.7400.94
256_T278_S0.7380.94
75_S105_L0.7370.94
243_Y274_Q0.7330.94
54_T58_E0.7310.94
240_V260_V0.7310.94
338_T359_A0.7290.94
104_S107_K0.7280.94
81_M88_E0.7270.94
338_T360_Y0.7250.94
85_L88_E0.7230.94
240_V287_M0.7230.94
79_L109_F0.7220.94
346_Q350_D0.7210.94
70_K74_E0.7190.93
336_H339_N0.7180.93
92_T96_L0.7160.93
165_L289_M0.7140.93
231_I291_K0.7120.93
93_D96_L0.7100.93
75_S79_L0.7090.93
27_E30_E0.7080.93
148_L162_V0.7080.93
152_T156_E0.7080.93
42_D47_N0.7040.93
25_D28_S0.7030.93
157_R354_D0.7010.93
253_V257_D0.7010.93
246_S256_T0.6980.92
329_P332_M0.6970.92
133_P141_Q0.6960.92
45_F55_V0.6960.92
255_T280_I0.6960.92
146_M349_M0.6950.92
330_Y334_K0.6950.92
90_P98_L0.6940.92
37_D41_A0.6920.92
239_K272_T0.6920.92
58_E61_G0.6920.92
31_A34_A0.6900.92
79_L83_H0.6900.92
64_D68_S0.6890.92
154_W158_R0.6880.92
244_R252_H0.6880.92
315_E320_S0.6860.92
263_T272_T0.6820.92
82_T86_L0.6820.92
358_D362_R0.6760.91
115_Q186_N0.6760.91
152_T178_V0.6750.91
83_H87_K0.6740.91
65_Y115_Q0.6740.91
333_V345_V0.6720.91
86_L98_L0.6720.91
86_L94_L0.6720.91
125_N225_F0.6710.91
71_K74_E0.6700.91
233_I262_I0.6670.91
197_V313_Q0.6670.91
45_F52_A0.6660.91
171_D321_Q0.6650.90
148_L167_Y0.6640.90
273_C285_R0.6630.90
338_T356_F0.6620.90
305_E309_I0.6620.90
330_Y341_E0.6610.90
56_I59_A0.6610.90
267_T294_L0.6600.90
255_T272_T0.6570.90
287_M291_K0.6550.90
25_D29_K0.6530.90
94_L97_E0.6520.90
82_T98_L0.6520.90
30_E66_V0.6510.90
45_F51_K0.6490.89
86_L102_L0.6460.89
79_L101_E0.6410.89
273_C289_M0.6380.89
231_I294_L0.6370.89
237_D266_P0.6370.89
75_S112_F0.6370.89
107_K110_N0.6360.88
135_A316_I0.6350.88
131_L148_L0.6330.88
154_W357_I0.6320.88
239_K287_M0.6320.88
77_E80_Q0.6310.88
31_A42_D0.6310.88
227_D302_Q0.6300.88
262_I271_V0.6290.88
166_D179_T0.6290.88
189_G361_L0.6270.88
225_F292_A0.6250.88
141_Q176_K0.6240.87
243_Y263_T0.6240.87
265_L269_V0.6220.87
138_T172_E0.6220.87
79_L106_T0.6210.87
35_E38_E0.6210.87
139_E213_H0.6210.87
150_M353_I0.6210.87
142_D145_S0.6190.87
245_A259_A0.6140.87
338_T341_E0.6120.86
248_A257_D0.6120.86
271_V289_M0.6110.86
145_S167_Y0.6100.86
343_G347_A0.6100.86
331_S341_E0.6090.86
342_M356_F0.6080.86
127_A188_Y0.6080.86
308_E311_G0.6080.86
271_V293_K0.6060.86
55_V59_A0.6040.86
84_D88_E0.6030.86
197_V314_K0.6030.86
61_G119_S0.6030.86
218_S316_I0.6020.86
29_K65_Y0.6010.85
356_F359_A0.6010.85
332_M336_H0.6000.85
82_T94_L0.5970.85
132_H218_S0.5960.85
40_M52_A0.5960.85
243_Y259_A0.5960.85
138_T171_D0.5960.85
153_R157_R0.5950.85
225_F295_Y0.5940.85
234_R237_D0.5930.85
228_E231_I0.5910.85
149_R153_R0.5900.84
183_K187_A0.5880.84
60_N63_K0.5870.84
83_H102_L0.5870.84
62_L66_V0.5860.84
76_H109_F0.5850.84
197_V216_F0.5830.84
38_E42_D0.5830.84
281_K285_R0.5830.84
306_L310_R0.5810.84
142_D327_F0.5810.84
285_R289_M0.5800.83
133_P167_Y0.5770.83
86_L95_Q0.5760.83
347_A350_D0.5670.82
209_S213_H0.5670.82
78_E82_T0.5650.82
255_T281_K0.5640.82
245_A272_T0.5630.82
187_A191_L0.5630.82
131_L217_V0.5620.81
51_K55_V0.5610.81
145_S172_E0.5610.81
118_L192_K0.5610.81
197_V320_S0.5600.81
134_G216_F0.5590.81
91_D94_L0.5570.81
252_H278_S0.5560.81
81_M97_E0.5550.81
166_D177_S0.5540.81
146_M205_P0.5540.81
54_T57_N0.5530.80
243_Y270_V0.5530.80
135_A317_G0.5520.80
168_L177_S0.5490.80
68_S112_F0.5480.80
82_T85_L0.5460.80
208_S213_H0.5420.79
225_F299_I0.5410.79
197_V218_S0.5400.79
140_S213_H0.5370.79
229_I299_I0.5370.79
294_L298_R0.5350.78
195_K313_Q0.5330.78
160_F190_Y0.5310.78
29_K62_L0.5300.78
218_S315_E0.5300.78
52_A59_A0.5290.77
146_M200_L0.5290.77
85_L98_L0.5290.77
237_D265_L0.5280.77
61_G124_K0.5240.77
109_F113_E0.5240.77
149_R152_T0.5230.77
326_V330_Y0.5230.77
271_V276_E0.5220.77
242_T255_T0.5220.77
309_I313_Q0.5210.76
144_G175_I0.5200.76
233_I291_K0.5190.76
228_E288_K0.5180.76
130_E211_R0.5180.76
220_E293_K0.5170.76
248_A283_R0.5170.76
133_P176_K0.5150.76
53_Q56_I0.5150.76
222_M292_A0.5140.75
316_I320_S0.5140.75
140_S197_V0.5130.75
240_V263_T0.5120.75
331_S336_H0.5120.75
260_V271_V0.5120.75
134_G197_V0.5120.75
242_T280_I0.5120.75
132_H220_E0.5110.75
84_D87_K0.5110.75
246_S272_T0.5110.75
30_E33_I0.5100.75
333_V342_M0.5100.75
216_F320_S0.5080.75
163_E281_K0.5070.74
140_S217_V0.5070.74
257_D280_I0.5060.74
135_A140_S0.5040.74
57_N124_K0.5040.74
307_D310_R0.5020.74
318_W324_S0.5010.74
140_S168_L0.5010.74
239_K256_T0.5010.74
82_T101_E0.5000.73
252_H275_T0.5000.73
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1zbtA 1 0.8415 100 0.127 Contact Map
2ihr1 1 0.9536 100 0.135 Contact Map
1gqeA 1 0.9891 100 0.136 Contact Map
3d5aX 1 0.929 100 0.138 Contact Map
2b3tB 1 0.8005 100 0.141 Contact Map
1rq0A 3 0.8415 100 0.192 Contact Map
2rsmA 1 0.3142 100 0.706 Contact Map
3j7yp 1 0.1585 100 0.745 Contact Map
1j26A 1 0.2377 100 0.795 Contact Map
4dh9Y 1 0.2596 99.9 0.807 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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