GREMLIN Database
TPIS - Triosephosphate isomerase
UniProt: P27876 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 253 (246)
Sequences: 4612 (2996)
Seq/√Len: 191.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
41_C62_G3.2431.00
135_E176_K3.1941.00
184_N227_D3.1671.00
139_T186_V2.7771.00
138_K141_D2.6641.00
239_E242_S2.3131.00
13_N71_S2.2861.00
44_A78_I2.2641.00
148_K193_T2.2201.00
143_V186_V2.1601.00
140_N189_H2.0931.00
125_I150_G2.0931.00
133_E142_L2.0591.00
113_A125_I2.0371.00
116_A121_I2.0161.00
106_D145_D1.9891.00
3_K37_E1.9471.00
216_P242_S1.9381.00
106_D149_K1.9171.00
60_K89_D1.9021.00
214_V231_A1.9011.00
74_F101_M1.8931.00
144_A189_H1.8901.00
155_S158_Q1.8801.00
133_E138_K1.8741.00
38_A247_L1.8731.00
200_Q204_D1.8491.00
66_M80_P1.8391.00
5_I163_V1.8281.00
131_L135_E1.8181.00
51_A61_V1.8181.00
83_L88_V1.7871.00
131_L176_K1.7601.00
70_E118_K1.7591.00
37_E207_R1.6911.00
181_K225_E1.6861.00
7_A124_I1.6771.00
63_A83_L1.6681.00
7_A39_V1.6671.00
66_M116_A1.6611.00
183_A222_Y1.6311.00
109_V125_I1.6221.00
68_F111_K1.6181.00
180_A221_E1.5871.00
117_F162_S1.5861.00
45_L78_I1.5801.00
5_I39_V1.5601.00
14_K18_E1.5571.00
41_C92_V1.5561.00
69_E111_K1.5431.00
91_C116_A1.5191.00
194_V206_L1.5191.00
140_N185_D1.5091.00
127_V143_V1.5081.00
130_T133_E1.5031.00
130_T169_I1.4911.00
29_S244_V1.4901.00
188_A192_K1.4501.00
122_V161_A1.4471.00
48_E52_S1.4401.00
69_E74_F1.4361.00
66_M93_I1.4301.00
23_V50_L1.4291.00
54_V61_V1.4181.00
21_S25_E1.4011.00
65_N102_F1.3971.00
110_N114_H1.3741.00
199_S202_A1.3721.00
6_I223_M1.3591.00
42_A64_Q1.3551.00
105_T108_T1.3471.00
214_V223_M1.3471.00
160_A205_K1.3421.00
144_A193_T1.3421.00
19_A50_L1.3411.00
195_A200_Q1.3331.00
92_V124_I1.3061.00
114_H118_K1.2911.00
65_N112_K1.2831.00
65_N77_E1.2781.00
19_A46_F1.2771.00
191_R208_I1.2771.00
148_K197_S1.2771.00
67_H102_F1.2721.00
70_E119_H1.2691.00
183_A228_I1.2511.00
68_F108_T1.2471.00
144_A148_K1.2381.00
15_T18_E1.2231.00
115_A118_K1.2221.00
42_A50_L1.2221.00
183_A225_E1.2181.00
100_E130_T1.2161.00
177_S222_Y1.2081.00
5_I209_Q1.2081.00
90_Y122_V1.2071.00
134_R182_D1.2021.00
39_V90_Y1.1691.00
127_V164_I1.1651.00
91_C121_I1.1641.00
77_E102_F1.1611.00
189_H193_T1.1601.00
63_A91_C1.1571.00
127_V147_V1.1541.00
80_P115_A1.1521.00
67_H115_A1.1441.00
184_N226_S1.1401.00
158_Q161_A1.1391.00
180_A183_A1.1301.00
80_P119_H1.1301.00
217_A242_S1.1261.00
22_F240_P1.1201.00
14_K19_A1.1091.00
195_A203_A1.1081.00
130_T142_L1.1081.00
41_C124_I1.1021.00
25_E241_Q1.0971.00
110_N153_G1.0891.00
15_T85_D1.0831.00
142_L169_I1.0791.00
47_L63_A1.0741.00
70_E115_A1.0721.00
115_A119_H1.0711.00
133_E145_D1.0681.00
43_P46_F1.0541.00
219_I223_M1.0531.00
114_H153_G1.0501.00
182_D185_D1.0401.00
68_F102_F1.0381.00
17_G85_D1.0351.00
195_A204_D1.0281.00
4_P223_M1.0281.00
219_I246_L1.0241.00
16_L49_K1.0071.00
223_M231_A0.9970.99
66_M91_C0.9970.99
132_E136_A0.9950.99
93_I113_A0.9950.99
245_Q248_E0.9890.99
181_K185_D0.9860.99
81_V119_H0.9860.99
215_K242_S0.9830.99
141_D144_A0.9820.99
52_S55_K0.9820.99
24_E28_S0.9800.99
4_P207_R0.9780.99
70_E81_V0.9770.99
54_V59_L0.9750.99
47_L61_V0.9710.99
24_E53_A0.9700.99
193_T196_E0.9680.99
214_V233_V0.9670.99
193_T197_S0.9670.99
5_I207_R0.9600.99
37_E60_K0.9580.99
156_E199_S0.9540.99
192_K195_A0.9500.99
191_R227_D0.9500.99
110_N150_G0.9500.99
49_K52_S0.9450.99
201_E205_K0.9400.99
12_M79_S0.9380.99
225_E228_I0.9100.99
184_N225_E0.9060.99
127_V169_I0.9040.99
189_H192_K0.9030.99
192_K196_E0.9020.99
89_D122_V0.9000.99
16_L46_F0.8970.99
84_K119_H0.8930.99
20_V53_A0.8930.99
222_Y228_I0.8830.99
16_L48_E0.8790.99
60_K90_Y0.8780.99
20_V24_E0.8760.99
15_T46_F0.8760.99
159_V162_S0.8740.99
27_K57_T0.8690.99
139_T143_V0.8690.99
63_A88_V0.8680.99
55_K58_D0.8620.99
71_S81_V0.8570.98
45_L48_E0.8570.98
145_D149_K0.8560.98
22_F26_V0.8540.98
43_P82_A0.8490.98
134_R185_D0.8460.98
23_V54_V0.8450.98
26_V240_P0.8380.98
24_E27_K0.8360.98
107_E111_K0.8350.98
17_G21_S0.8320.98
90_Y123_P0.8300.98
140_N144_A0.8270.98
147_V190_I0.8260.98
51_A86_L0.8250.98
100_E169_I0.8200.98
117_F161_A0.8200.98
113_A162_S0.8160.98
159_V206_L0.8120.98
154_L158_Q0.8120.98
61_V88_V0.8100.98
25_E240_P0.8090.98
80_P116_A0.8090.98
25_E29_S0.8070.98
122_V163_V0.8060.98
103_A108_T0.7950.98
46_F82_A0.7950.98
188_A227_D0.7890.97
45_L86_L0.7890.97
20_V49_K0.7870.97
148_K152_A0.7850.97
113_A150_G0.7840.97
14_K22_F0.7820.97
190_I208_I0.7820.97
69_E102_F0.7790.97
127_V190_I0.7770.97
244_V248_E0.7740.97
222_Y231_A0.7740.97
14_K71_S0.7700.97
6_I219_I0.7680.97
113_A117_F0.7660.97
77_E98_R0.7650.97
48_E86_L0.7630.97
233_V246_L0.7610.97
181_K221_E0.7600.97
93_I116_A0.7590.97
50_L61_V0.7580.97
23_V53_A0.7550.97
159_V202_A0.7510.96
107_E149_K0.7490.96
84_K120_G0.7450.96
216_P245_Q0.7410.96
241_Q245_Q0.7370.96
144_A196_E0.7360.96
188_A226_S0.7340.96
19_A22_F0.7270.96
219_I231_A0.7240.96
184_N188_A0.7240.96
131_L174_T0.7220.96
164_I190_I0.7200.96
195_A199_S0.7170.95
154_L162_S0.7130.95
27_K53_A0.7110.95
37_E122_V0.7060.95
242_S245_Q0.7040.95
7_A41_C0.7030.95
29_S241_Q0.7000.95
143_V190_I0.7000.95
62_G90_Y0.6970.95
191_R229_D0.6950.95
19_A49_K0.6950.95
150_G153_G0.6940.95
22_F50_L0.6910.94
114_H158_Q0.6900.94
223_M249_E0.6900.94
39_V124_I0.6870.94
27_K56_G0.6860.94
16_L85_D0.6830.94
149_K152_A0.6820.94
51_A54_V0.6800.94
45_L82_A0.6750.94
116_A123_P0.6740.94
140_N186_V0.6720.94
38_A42_A0.6720.94
90_Y163_V0.6690.93
27_K55_K0.6660.93
143_V189_H0.6620.93
178_S222_Y0.6580.93
215_K218_N0.6570.93
108_T111_K0.6570.93
18_E21_S0.6570.93
66_M119_H0.6510.93
180_A225_E0.6480.92
141_D145_D0.6430.92
51_A89_D0.6430.92
156_E202_A0.6360.92
178_S183_A0.6340.92
19_A23_V0.6310.91
4_P229_D0.6310.91
76_G98_R0.6300.91
117_F154_L0.6250.91
78_I82_A0.6250.91
80_P121_I0.6220.91
26_V40_V0.6180.90
123_P159_V0.6180.90
39_V163_V0.6140.90
187_C228_I0.6120.90
125_I162_S0.6100.90
219_I249_E0.6080.90
159_V198_F0.6080.90
26_V244_V0.6080.90
39_V62_G0.6070.90
3_K207_R0.6070.90
110_N154_L0.6070.90
151_L159_V0.6020.89
51_A87_G0.6010.89
111_K153_G0.6000.89
67_H108_T0.5960.89
26_V30_I0.5950.89
29_S248_E0.5880.88
156_E159_V0.5870.88
66_M83_L0.5860.88
43_P78_I0.5860.88
125_I147_V0.5840.88
49_K53_A0.5830.88
67_H101_M0.5810.88
147_V193_T0.5770.87
40_V50_L0.5750.87
46_F85_D0.5740.87
90_Y124_I0.5720.87
140_N143_V0.5720.87
14_K46_F0.5710.87
187_C219_I0.5690.86
220_K224_A0.5690.86
113_A154_L0.5670.86
9_N232_L0.5650.86
142_L145_D0.5650.86
22_F25_E0.5650.86
38_A243_F0.5640.86
32_A248_E0.5640.86
71_S79_S0.5620.86
26_V59_L0.5620.86
235_G238_L0.5620.86
68_F112_K0.5590.86
218_N222_Y0.5540.85
233_V237_S0.5510.85
74_F102_F0.5510.85
22_F40_V0.5480.84
50_L54_V0.5470.84
221_E224_A0.5460.84
174_T177_S0.5440.84
177_S180_A0.5430.84
25_E28_S0.5410.84
33_A36_A0.5390.83
51_A88_V0.5370.83
93_I109_V0.5350.83
109_V150_G0.5350.83
66_M121_I0.5340.83
244_V247_L0.5340.83
33_A58_D0.5340.83
127_V186_V0.5330.83
217_A221_E0.5310.83
70_E80_P0.5280.82
20_V52_S0.5270.82
138_K145_D0.5260.82
51_A55_K0.5240.82
83_L86_L0.5230.82
217_A245_Q0.5220.81
12_M67_H0.5210.81
20_V27_K0.5190.81
54_V57_T0.5180.81
177_S231_A0.5150.81
66_M82_A0.5150.81
155_S162_S0.5140.80
215_K239_E0.5130.80
73_A235_G0.5130.80
114_H152_A0.5120.80
28_S31_P0.5090.80
47_L83_L0.5070.80
105_T109_V0.5070.80
21_S24_E0.5050.79
179_T182_D0.5030.79
39_V42_A0.5030.79
182_D222_Y0.5030.79
240_P244_V0.5030.79
84_K89_D0.5010.79
77_E101_M0.5000.79
67_H74_F0.5000.79
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3m9yA 2 0.9881 100 0.052 Contact Map
1aw2A 2 0.9921 100 0.052 Contact Map
2btmA 2 0.9881 100 0.054 Contact Map
1yyaA 2 0.9881 100 0.055 Contact Map
2j27A 2 0.9802 100 0.056 Contact Map
3ta6A 2 0.996 100 0.056 Contact Map
1m6jA 2 0.996 100 0.057 Contact Map
1o5xA 2 0.9723 100 0.059 Contact Map
4mvaA 2 0.9921 100 0.06 Contact Map
1b9bA 3 0.996 100 0.064 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0148 seconds.