TPIS - BACSU - GREMLIN Contacts

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TPIS - Triosephosphate isomerase

UniProt:	P27876	Sequences: MRKPIIAGNWKMNKTLGEAVSFVEEVKSSIPAADKAEAVVCAPALFLEKLASAVKGTDLKVGAQNMHFEESGAFTGEISPVALKDLGVDYCVIGHSERREMFAETDETVNKKAHAAFKHGIVPIICVGETLEEREAGKTNDLVADQVKKGLAGLSEEQVAASVIAYEPIWAIGTGKSSTAKDANDVCAHIRKTVAESFSQEAADKLRIQYGGSVKPANIKEYMAESDIDGALVGGASLEPQSFVQLLEEGQYE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	253 (246)
Sequences:	4612 (2996)
Seq/√Len:	191.0

ECOLI - TPIS
BACSU - TPIS

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
41_C	62_G	3.243	1.00
135_E	176_K	3.194	1.00
184_N	227_D	3.167	1.00
139_T	186_V	2.777	1.00
138_K	141_D	2.664	1.00
239_E	242_S	2.313	1.00
13_N	71_S	2.286	1.00
44_A	78_I	2.264	1.00
148_K	193_T	2.220	1.00
143_V	186_V	2.160	1.00
140_N	189_H	2.093	1.00
125_I	150_G	2.093	1.00
133_E	142_L	2.059	1.00
113_A	125_I	2.037	1.00
116_A	121_I	2.016	1.00
106_D	145_D	1.989	1.00
3_K	37_E	1.947	1.00
216_P	242_S	1.938	1.00
106_D	149_K	1.917	1.00
60_K	89_D	1.902	1.00
214_V	231_A	1.901	1.00
74_F	101_M	1.893	1.00
144_A	189_H	1.890	1.00
155_S	158_Q	1.880	1.00
133_E	138_K	1.874	1.00
38_A	247_L	1.873	1.00
200_Q	204_D	1.849	1.00
66_M	80_P	1.839	1.00
5_I	163_V	1.828	1.00
131_L	135_E	1.818	1.00
51_A	61_V	1.818	1.00
83_L	88_V	1.787	1.00
131_L	176_K	1.760	1.00
70_E	118_K	1.759	1.00
37_E	207_R	1.691	1.00
181_K	225_E	1.686	1.00
7_A	124_I	1.677	1.00
63_A	83_L	1.668	1.00
7_A	39_V	1.667	1.00
66_M	116_A	1.661	1.00
183_A	222_Y	1.631	1.00
109_V	125_I	1.622	1.00
68_F	111_K	1.618	1.00
180_A	221_E	1.587	1.00
117_F	162_S	1.586	1.00
45_L	78_I	1.580	1.00
5_I	39_V	1.560	1.00
14_K	18_E	1.557	1.00
41_C	92_V	1.556	1.00
69_E	111_K	1.543	1.00
91_C	116_A	1.519	1.00
194_V	206_L	1.519	1.00
140_N	185_D	1.509	1.00
127_V	143_V	1.508	1.00
130_T	133_E	1.503	1.00
130_T	169_I	1.491	1.00
29_S	244_V	1.490	1.00
188_A	192_K	1.450	1.00
122_V	161_A	1.447	1.00
48_E	52_S	1.440	1.00
69_E	74_F	1.436	1.00
66_M	93_I	1.430	1.00
23_V	50_L	1.429	1.00
54_V	61_V	1.418	1.00
21_S	25_E	1.401	1.00
65_N	102_F	1.397	1.00
110_N	114_H	1.374	1.00
199_S	202_A	1.372	1.00
6_I	223_M	1.359	1.00
42_A	64_Q	1.355	1.00
105_T	108_T	1.347	1.00
214_V	223_M	1.347	1.00
160_A	205_K	1.342	1.00
144_A	193_T	1.342	1.00
19_A	50_L	1.341	1.00
195_A	200_Q	1.333	1.00
92_V	124_I	1.306	1.00
114_H	118_K	1.291	1.00
65_N	112_K	1.283	1.00
65_N	77_E	1.278	1.00
19_A	46_F	1.277	1.00
191_R	208_I	1.277	1.00
148_K	197_S	1.277	1.00
67_H	102_F	1.272	1.00
70_E	119_H	1.269	1.00
183_A	228_I	1.251	1.00
68_F	108_T	1.247	1.00
144_A	148_K	1.238	1.00
15_T	18_E	1.223	1.00
115_A	118_K	1.222	1.00
42_A	50_L	1.222	1.00
183_A	225_E	1.218	1.00
100_E	130_T	1.216	1.00
177_S	222_Y	1.208	1.00
5_I	209_Q	1.208	1.00
90_Y	122_V	1.207	1.00
134_R	182_D	1.202	1.00
39_V	90_Y	1.169	1.00
127_V	164_I	1.165	1.00
91_C	121_I	1.164	1.00
77_E	102_F	1.161	1.00
189_H	193_T	1.160	1.00
63_A	91_C	1.157	1.00
127_V	147_V	1.154	1.00
80_P	115_A	1.152	1.00
67_H	115_A	1.144	1.00
184_N	226_S	1.140	1.00
158_Q	161_A	1.139	1.00
180_A	183_A	1.130	1.00
80_P	119_H	1.130	1.00
217_A	242_S	1.126	1.00
22_F	240_P	1.120	1.00
14_K	19_A	1.109	1.00
195_A	203_A	1.108	1.00
130_T	142_L	1.108	1.00
41_C	124_I	1.102	1.00
25_E	241_Q	1.097	1.00
110_N	153_G	1.089	1.00
15_T	85_D	1.083	1.00
142_L	169_I	1.079	1.00
47_L	63_A	1.074	1.00
70_E	115_A	1.072	1.00
115_A	119_H	1.071	1.00
133_E	145_D	1.068	1.00
43_P	46_F	1.054	1.00
219_I	223_M	1.053	1.00
114_H	153_G	1.050	1.00
182_D	185_D	1.040	1.00
68_F	102_F	1.038	1.00
17_G	85_D	1.035	1.00
195_A	204_D	1.028	1.00
4_P	223_M	1.028	1.00
219_I	246_L	1.024	1.00
16_L	49_K	1.007	1.00
223_M	231_A	0.997	0.99
66_M	91_C	0.997	0.99
132_E	136_A	0.995	0.99
93_I	113_A	0.995	0.99
245_Q	248_E	0.989	0.99
181_K	185_D	0.986	0.99
81_V	119_H	0.986	0.99
215_K	242_S	0.983	0.99
141_D	144_A	0.982	0.99
52_S	55_K	0.982	0.99
24_E	28_S	0.980	0.99
4_P	207_R	0.978	0.99
70_E	81_V	0.977	0.99
54_V	59_L	0.975	0.99
47_L	61_V	0.971	0.99
24_E	53_A	0.970	0.99
193_T	196_E	0.968	0.99
214_V	233_V	0.967	0.99
193_T	197_S	0.967	0.99
5_I	207_R	0.960	0.99
37_E	60_K	0.958	0.99
156_E	199_S	0.954	0.99
192_K	195_A	0.950	0.99
191_R	227_D	0.950	0.99
110_N	150_G	0.950	0.99
49_K	52_S	0.945	0.99
201_E	205_K	0.940	0.99
12_M	79_S	0.938	0.99
225_E	228_I	0.910	0.99
184_N	225_E	0.906	0.99
127_V	169_I	0.904	0.99
189_H	192_K	0.903	0.99
192_K	196_E	0.902	0.99
89_D	122_V	0.900	0.99
16_L	46_F	0.897	0.99
84_K	119_H	0.893	0.99
20_V	53_A	0.893	0.99
222_Y	228_I	0.883	0.99
16_L	48_E	0.879	0.99
60_K	90_Y	0.878	0.99
20_V	24_E	0.876	0.99
15_T	46_F	0.876	0.99
159_V	162_S	0.874	0.99
27_K	57_T	0.869	0.99
139_T	143_V	0.869	0.99
63_A	88_V	0.868	0.99
55_K	58_D	0.862	0.99
71_S	81_V	0.857	0.98
45_L	48_E	0.857	0.98
145_D	149_K	0.856	0.98
22_F	26_V	0.854	0.98
43_P	82_A	0.849	0.98
134_R	185_D	0.846	0.98
23_V	54_V	0.845	0.98
26_V	240_P	0.838	0.98
24_E	27_K	0.836	0.98
107_E	111_K	0.835	0.98
17_G	21_S	0.832	0.98
90_Y	123_P	0.830	0.98
140_N	144_A	0.827	0.98
147_V	190_I	0.826	0.98
51_A	86_L	0.825	0.98
100_E	169_I	0.820	0.98
117_F	161_A	0.820	0.98
113_A	162_S	0.816	0.98
159_V	206_L	0.812	0.98
154_L	158_Q	0.812	0.98
61_V	88_V	0.810	0.98
25_E	240_P	0.809	0.98
80_P	116_A	0.809	0.98
25_E	29_S	0.807	0.98
122_V	163_V	0.806	0.98
103_A	108_T	0.795	0.98
46_F	82_A	0.795	0.98
188_A	227_D	0.789	0.97
45_L	86_L	0.789	0.97
20_V	49_K	0.787	0.97
148_K	152_A	0.785	0.97
113_A	150_G	0.784	0.97
14_K	22_F	0.782	0.97
190_I	208_I	0.782	0.97
69_E	102_F	0.779	0.97
127_V	190_I	0.777	0.97
244_V	248_E	0.774	0.97
222_Y	231_A	0.774	0.97
14_K	71_S	0.770	0.97
6_I	219_I	0.768	0.97
113_A	117_F	0.766	0.97
77_E	98_R	0.765	0.97
48_E	86_L	0.763	0.97
233_V	246_L	0.761	0.97
181_K	221_E	0.760	0.97
93_I	116_A	0.759	0.97
50_L	61_V	0.758	0.97
23_V	53_A	0.755	0.97
159_V	202_A	0.751	0.96
107_E	149_K	0.749	0.96
84_K	120_G	0.745	0.96
216_P	245_Q	0.741	0.96
241_Q	245_Q	0.737	0.96
144_A	196_E	0.736	0.96
188_A	226_S	0.734	0.96
19_A	22_F	0.727	0.96
219_I	231_A	0.724	0.96
184_N	188_A	0.724	0.96
131_L	174_T	0.722	0.96
164_I	190_I	0.720	0.96
195_A	199_S	0.717	0.95
154_L	162_S	0.713	0.95
27_K	53_A	0.711	0.95
37_E	122_V	0.706	0.95
242_S	245_Q	0.704	0.95
7_A	41_C	0.703	0.95
29_S	241_Q	0.700	0.95
143_V	190_I	0.700	0.95
62_G	90_Y	0.697	0.95
191_R	229_D	0.695	0.95
19_A	49_K	0.695	0.95
150_G	153_G	0.694	0.95
22_F	50_L	0.691	0.94
114_H	158_Q	0.690	0.94
223_M	249_E	0.690	0.94
39_V	124_I	0.687	0.94
27_K	56_G	0.686	0.94
16_L	85_D	0.683	0.94
149_K	152_A	0.682	0.94
51_A	54_V	0.680	0.94
45_L	82_A	0.675	0.94
116_A	123_P	0.674	0.94
140_N	186_V	0.672	0.94
38_A	42_A	0.672	0.94
90_Y	163_V	0.669	0.93
27_K	55_K	0.666	0.93
143_V	189_H	0.662	0.93
178_S	222_Y	0.658	0.93
215_K	218_N	0.657	0.93
108_T	111_K	0.657	0.93
18_E	21_S	0.657	0.93
66_M	119_H	0.651	0.93
180_A	225_E	0.648	0.92
141_D	145_D	0.643	0.92
51_A	89_D	0.643	0.92
156_E	202_A	0.636	0.92
178_S	183_A	0.634	0.92
19_A	23_V	0.631	0.91
4_P	229_D	0.631	0.91
76_G	98_R	0.630	0.91
117_F	154_L	0.625	0.91
78_I	82_A	0.625	0.91
80_P	121_I	0.622	0.91
26_V	40_V	0.618	0.90
123_P	159_V	0.618	0.90
39_V	163_V	0.614	0.90
187_C	228_I	0.612	0.90
125_I	162_S	0.610	0.90
219_I	249_E	0.608	0.90
159_V	198_F	0.608	0.90
26_V	244_V	0.608	0.90
39_V	62_G	0.607	0.90
3_K	207_R	0.607	0.90
110_N	154_L	0.607	0.90
151_L	159_V	0.602	0.89
51_A	87_G	0.601	0.89
111_K	153_G	0.600	0.89
67_H	108_T	0.596	0.89
26_V	30_I	0.595	0.89
29_S	248_E	0.588	0.88
156_E	159_V	0.587	0.88
66_M	83_L	0.586	0.88
43_P	78_I	0.586	0.88
125_I	147_V	0.584	0.88
49_K	53_A	0.583	0.88
67_H	101_M	0.581	0.88
147_V	193_T	0.577	0.87
40_V	50_L	0.575	0.87
46_F	85_D	0.574	0.87
90_Y	124_I	0.572	0.87
140_N	143_V	0.572	0.87
14_K	46_F	0.571	0.87
187_C	219_I	0.569	0.86
220_K	224_A	0.569	0.86
113_A	154_L	0.567	0.86
9_N	232_L	0.565	0.86
142_L	145_D	0.565	0.86
22_F	25_E	0.565	0.86
38_A	243_F	0.564	0.86
32_A	248_E	0.564	0.86
71_S	79_S	0.562	0.86
26_V	59_L	0.562	0.86
235_G	238_L	0.562	0.86
68_F	112_K	0.559	0.86
218_N	222_Y	0.554	0.85
233_V	237_S	0.551	0.85
74_F	102_F	0.551	0.85
22_F	40_V	0.548	0.84
50_L	54_V	0.547	0.84
221_E	224_A	0.546	0.84
174_T	177_S	0.544	0.84
177_S	180_A	0.543	0.84
25_E	28_S	0.541	0.84
33_A	36_A	0.539	0.83
51_A	88_V	0.537	0.83
93_I	109_V	0.535	0.83
109_V	150_G	0.535	0.83
66_M	121_I	0.534	0.83
244_V	247_L	0.534	0.83
33_A	58_D	0.534	0.83
127_V	186_V	0.533	0.83
217_A	221_E	0.531	0.83
70_E	80_P	0.528	0.82
20_V	52_S	0.527	0.82
138_K	145_D	0.526	0.82
51_A	55_K	0.524	0.82
83_L	86_L	0.523	0.82
217_A	245_Q	0.522	0.81
12_M	67_H	0.521	0.81
20_V	27_K	0.519	0.81
54_V	57_T	0.518	0.81
177_S	231_A	0.515	0.81
66_M	82_A	0.515	0.81
155_S	162_S	0.514	0.80
215_K	239_E	0.513	0.80
73_A	235_G	0.513	0.80
114_H	152_A	0.512	0.80
28_S	31_P	0.509	0.80
47_L	83_L	0.507	0.80
105_T	109_V	0.507	0.80
21_S	24_E	0.505	0.79
179_T	182_D	0.503	0.79
39_V	42_A	0.503	0.79
182_D	222_Y	0.503	0.79
240_P	244_V	0.503	0.79
84_K	89_D	0.501	0.79
77_E	101_M	0.500	0.79
67_H	74_F	0.500	0.79

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3m9yA	2	0.9881	100	0.052	Contact Map
1aw2A	2	0.9921	100	0.052	Contact Map
2btmA	2	0.9881	100	0.054	Contact Map
1yyaA	2	0.9881	100	0.055	Contact Map
2j27A	2	0.9802	100	0.056	Contact Map
3ta6A	2	0.996	100	0.056	Contact Map
1m6jA	2	0.996	100	0.057	Contact Map
1o5xA	2	0.9723	100	0.059	Contact Map
4mvaA	2	0.9921	100	0.06	Contact Map
1b9bA	3	0.996	100	0.064	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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