GREMLIN Database
SP4FA - Stage IV sporulation protein FA
UniProt: P26936 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 264 (226)
Sequences: 4114 (3734)
Seq/√Len: 248.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
214_L232_G3.9961.00
189_V217_V3.7961.00
173_T234_I3.2881.00
203_H209_S3.2201.00
218_D233_S3.1661.00
236_L239_H3.1151.00
178_I214_L2.9271.00
161_D258_I2.7911.00
159_Q172_E2.7341.00
177_K230_K2.7211.00
152_A171_V2.6461.00
181_V246_F2.5761.00
175_S242_G2.4511.00
183_E226_D2.2661.00
162_F254_F2.2241.00
185_Y224_F2.2081.00
151_I227_K2.1871.00
180_S201_V2.1451.00
105_V109_F2.0501.00
195_T212_G1.9891.00
219_V223_D1.9381.00
167_E247_A1.9231.00
214_L217_V1.8091.00
110_E114_Q1.7741.00
107_K111_T1.7371.00
178_I234_I1.6821.00
104_A108_T1.6741.00
230_K233_S1.6621.00
112_E116_A1.6591.00
108_T112_E1.6501.00
201_V231_L1.6441.00
78_L82_C1.6421.00
200_K210_I1.6191.00
207_T250_D1.6171.00
187_V208_Y1.6151.00
114_Q118_A1.6141.00
209_S248_M1.6111.00
157_K172_E1.6051.00
121_W125_K1.6031.00
181_V209_S1.6001.00
116_A119_S1.5811.00
111_T114_Q1.5781.00
109_F113_F1.5561.00
121_W124_T1.5551.00
111_T115_F1.5401.00
173_T176_D1.5371.00
106_A110_E1.5071.00
38_S41_M1.5061.00
170_K245_Y1.5011.00
179_D230_K1.4931.00
226_D229_K1.4861.00
220_A223_D1.4541.00
118_A121_W1.4441.00
199_V214_L1.4301.00
130_L134_A1.4251.00
200_K208_Y1.4081.00
187_V202_Q1.3971.00
234_I242_G1.3301.00
212_G247_A1.3271.00
115_F119_S1.3171.00
119_S123_E1.3121.00
181_V211_Y1.3111.00
166_G170_K1.2821.00
156_G171_V1.2671.00
120_H124_T1.2561.00
175_S215_K1.2551.00
182_K227_K1.2531.00
219_V229_K1.2501.00
131_A135_P1.2471.00
199_V231_L1.2251.00
218_D232_G1.2191.00
98_V102_K1.2151.00
62_N66_P1.1991.00
234_I244_Y1.1981.00
117_S120_H1.1971.00
94_N98_V1.1901.00
249_K254_F1.1731.00
169_I258_I1.1731.00
35_K39_W1.1611.00
170_K243_V1.1571.00
56_D59_P1.1571.00
60_T64_K1.1501.00
103_P107_K1.1451.00
132_F135_P1.1311.00
109_F112_E1.1251.00
198_T210_I1.1231.00
91_Y94_N1.1111.00
118_A122_F1.1061.00
108_T111_T1.1031.00
78_L81_A1.0971.00
192_D198_T1.0971.00
106_A109_F1.0961.00
64_K68_V1.0961.00
70_T73_I1.0961.00
99_S102_K1.0941.00
39_W43_T1.0911.00
90_A94_N1.0911.00
117_S121_W1.0901.00
113_F117_S1.0871.00
166_G243_V1.0791.00
216_D233_S1.0771.00
97_P101_I1.0761.00
160_Q166_G1.0711.00
93_T97_P1.0711.00
256_D259_Q1.0661.00
46_E50_T1.0661.00
114_Q117_S1.0621.00
105_V108_T1.0581.00
68_V71_D1.0581.00
131_A134_A1.0561.00
94_N97_P1.0461.00
198_T212_G1.0421.00
49_G53_V1.0381.00
55_E59_P1.0351.00
195_T247_A1.0281.00
53_V57_N1.0241.00
36_P40_V1.0191.00
91_Y95_I1.0191.00
188_E198_T0.9931.00
82_C85_L0.9921.00
76_K80_S0.9841.00
71_D75_L0.9741.00
191_K197_L0.9711.00
164_D238_D0.9681.00
170_K213_E0.9671.00
155_S179_D0.9641.00
126_F130_L0.9631.00
101_I105_V0.9591.00
176_D234_I0.9481.00
158_V169_I0.9481.00
80_S83_L0.9411.00
34_Q38_S0.9401.00
54_Y58_M0.9361.00
177_K233_S0.9351.00
97_P100_Q0.9231.00
185_Y202_Q0.9211.00
45_Q48_H0.9181.00
90_A93_T0.9181.00
60_T63_G0.9171.00
213_E237_D0.9161.00
152_A261_I0.9121.00
120_H123_E0.9111.00
218_D230_K0.9111.00
188_E200_K0.9061.00
194_Q212_G0.9021.00
95_I99_S0.8981.00
67_L71_D0.8961.00
47_K50_T0.8961.00
154_A171_V0.8931.00
86_V90_A0.8921.00
49_G52_P0.8911.00
152_A155_S0.8821.00
150_L207_T0.8801.00
123_E126_F0.8791.00
169_I261_I0.8771.00
207_T248_M0.8751.00
30_V33_K0.8741.00
92_K96_G0.8720.99
255_I260_V0.8670.99
96_G100_Q0.8610.99
101_I104_A0.8610.99
59_P62_N0.8600.99
184_G201_V0.8600.99
56_D60_T0.8590.99
43_T46_E0.8570.99
43_T47_K0.8560.99
236_L240_N0.8540.99
130_L133_L0.8500.99
73_I77_C0.8450.99
154_A173_T0.8410.99
198_T214_L0.8390.99
215_K233_S0.8370.99
89_I92_K0.8330.99
52_P56_D0.8320.99
33_K36_P0.8230.99
57_N61_F0.8230.99
195_T210_I0.8210.99
81_A85_L0.8200.99
37_P41_M0.8180.99
247_A254_F0.8130.99
107_K110_E0.8120.99
51_L54_Y0.8100.99
110_E113_F0.8090.99
236_L242_G0.8050.99
202_Q206_N0.8010.99
40_V43_T0.7980.99
195_T249_K0.7970.99
67_L70_T0.7970.99
250_D255_I0.7910.99
87_S91_Y0.7910.99
38_S42_V0.7840.99
92_K95_I0.7750.99
46_E49_G0.7750.99
167_E254_F0.7700.99
52_P55_E0.7660.99
66_P69_K0.7650.99
183_E227_K0.7640.99
41_M45_Q0.7600.99
77_C80_S0.7580.99
63_G66_P0.7550.99
160_Q163_Q0.7460.98
79_L82_C0.7420.98
35_K38_S0.7380.98
161_D256_D0.7360.98
48_H51_L0.7330.98
42_V46_E0.7310.98
88_A92_K0.7280.98
104_A107_K0.7260.98
69_K73_I0.7260.98
196_G235_K0.7250.98
143_Q146_V0.7190.98
122_F126_F0.7190.98
85_L89_I0.7170.98
115_F118_A0.7120.98
79_L83_L0.7120.98
48_H52_P0.7110.98
143_Q147_G0.7080.98
214_L244_Y0.7030.98
51_L55_E0.7010.98
33_K37_P0.6990.98
213_E239_H0.6990.98
45_Q49_G0.6960.98
83_L86_V0.6950.98
61_F64_K0.6940.97
199_V219_V0.6910.97
74_I78_L0.6900.97
112_E119_S0.6900.97
103_P106_A0.6900.97
50_T54_Y0.6860.97
158_V258_I0.6860.97
159_Q241_K0.6830.97
89_I93_T0.6820.97
36_P39_W0.6810.97
119_S122_F0.6790.97
188_E192_D0.6740.97
98_V101_I0.6720.97
40_V44_D0.6710.97
113_F116_A0.6680.97
66_P70_T0.6660.97
77_C81_A0.6650.97
95_I98_V0.6600.97
59_P63_G0.6600.97
50_T53_V0.6570.97
84_V87_S0.6540.96
116_A120_H0.6530.96
214_L218_D0.6510.96
151_I181_V0.6500.96
209_S246_F0.6470.96
70_T74_I0.6430.96
88_A98_V0.6410.96
54_Y57_N0.6360.96
154_A234_I0.6320.96
195_T198_T0.6290.96
171_V261_I0.6270.95
159_Q243_V0.6260.95
42_V45_Q0.6240.95
63_G67_L0.6220.95
208_Y249_K0.6190.95
210_I249_K0.6180.95
93_T96_G0.6180.95
212_G249_K0.6160.95
73_I76_K0.6140.95
72_S76_K0.6140.95
60_T67_L0.6120.95
197_L217_V0.6110.95
85_L88_A0.6060.95
202_Q208_Y0.6060.95
183_E224_F0.6040.94
166_G213_E0.6040.94
93_T98_V0.6000.94
65_H68_V0.6000.94
150_L182_K0.6000.94
158_V161_D0.5950.94
169_I257_P0.5950.94
182_K203_H0.5940.94
154_A176_D0.5910.94
203_H211_Y0.5910.94
67_L74_I0.5890.94
149_D182_K0.5840.93
235_K243_V0.5800.93
147_G182_K0.5800.93
88_A91_Y0.5780.93
115_F122_F0.5770.93
100_Q103_P0.5730.93
50_T81_A0.5730.93
61_F65_H0.5700.93
178_I232_G0.5640.92
72_S75_L0.5630.92
151_I179_D0.5630.92
57_N60_T0.5630.92
213_E245_Y0.5620.92
214_L234_I0.5600.92
164_D167_E0.5600.92
83_L87_S0.5590.92
182_K207_T0.5580.92
210_I247_A0.5570.92
58_M62_N0.5520.91
80_S84_V0.5520.91
192_D200_K0.5510.91
96_G99_S0.5510.91
99_S103_P0.5510.91
166_G245_Y0.5510.91
35_K77_C0.5500.91
156_G248_M0.5480.91
172_E241_K0.5480.91
160_Q165_N0.5480.91
34_Q37_P0.5460.91
146_V182_K0.5450.91
211_Y246_F0.5450.91
57_N64_K0.5430.91
75_L79_L0.5420.91
135_P139_N0.5380.90
213_E235_K0.5370.90
167_E249_K0.5360.90
65_H69_K0.5350.90
44_D47_K0.5350.90
160_Q170_K0.5340.90
114_Q122_F0.5290.90
102_K106_A0.5290.90
203_H246_F0.5270.89
75_L78_L0.5270.89
150_L260_V0.5260.89
178_I244_Y0.5230.89
113_F118_A0.5230.89
200_K249_K0.5230.89
166_G239_H0.5230.89
140_K143_Q0.5170.89
41_M44_D0.5130.88
159_Q240_N0.5130.88
126_F132_F0.5130.88
213_E241_K0.5120.88
166_G241_K0.5070.88
159_Q239_H0.5060.87
258_I261_I0.5060.87
255_I259_Q0.5050.87
143_Q260_V0.5020.87
31_S34_Q0.5020.87
195_T200_K0.5010.87
49_G56_D0.5000.87
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4bh5A 4 0.4621 100 0.403 Contact Map
2hsiA 1 0.7159 100 0.414 Contact Map
3tufB 1 0.4508 100 0.419 Contact Map
4rnzA 1 0.9432 100 0.434 Contact Map
2gu1A 1 0.9394 100 0.447 Contact Map
3sluA 1 0.9735 100 0.454 Contact Map
1qwyA 1 0.5682 100 0.458 Contact Map
3it5A 1 0.4394 100 0.473 Contact Map
3nyyA 2 0.7803 100 0.474 Contact Map
4qp5A 1 0.447 100 0.483 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0085 seconds.