GREMLIN Database
ASPG1 - L-asparaginase 1
UniProt: P26900 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 329 (314)
Sequences: 3441 (2510)
Seq/√Len: 141.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
95_L108_I4.4371.00
3_K43_T4.2051.00
220_D252_E4.1851.00
209_K232_E3.7971.00
91_T108_I3.6891.00
47_Q68_A3.4831.00
99_L106_I3.3951.00
142_V154_A3.2831.00
220_D249_K3.0551.00
5_L76_Y3.0141.00
314_V317_R2.9991.00
311_D314_V2.9661.00
7_L49_L2.6661.00
52_I61_Y2.6601.00
149_I167_S2.4381.00
305_A318_I2.4381.00
228_G302_L2.3331.00
80_V133_A2.2971.00
45_E76_Y2.2861.00
3_K45_E2.2591.00
143_V148_V2.2281.00
206_C226_Y2.2261.00
73_Y104_K2.2121.00
69_V81_I2.2101.00
251_N255_E2.2081.00
59_P213_G2.1991.00
232_E295_T2.1951.00
263_T290_S2.1781.00
73_Y102_A2.1551.00
8_T14_A2.1521.00
73_Y79_F2.1501.00
149_I154_A2.1221.00
157_L166_E2.1191.00
99_L102_A2.0741.00
261_V319_M2.0051.00
5_L72_N1.9801.00
148_V174_A1.9441.00
128_D146_G1.9181.00
132_F148_V1.9011.00
88_M142_V1.8901.00
66_A98_M1.8661.00
262_I287_I1.8531.00
58_Q61_Y1.8401.00
28_A46_T1.8401.00
50_M61_Y1.8321.00
7_L68_A1.8071.00
305_A315_V1.8051.00
56_N241_F1.7911.00
62_W212_P1.7751.00
312_L316_K1.7661.00
96_S152_T1.7611.00
261_V290_S1.7371.00
233_S262_I1.7261.00
7_L47_Q1.6791.00
222_L253_L1.6731.00
202_C227_K1.6611.00
216_P220_D1.6511.00
18_G23_A1.6501.00
59_P303_M1.6311.00
72_N75_A1.6221.00
57_M61_Y1.6121.00
312_L315_V1.6021.00
62_W300_P1.6011.00
128_D143_V1.6001.00
109_T144_F1.5801.00
261_V302_L1.5711.00
157_L164_A1.5641.00
249_K252_E1.5481.00
94_A98_M1.5271.00
83_H91_T1.5181.00
207_L295_T1.5141.00
204_D215_K1.4931.00
29_D33_S1.4831.00
216_P249_K1.4661.00
127_T131_R1.4631.00
69_V106_I1.4421.00
219_F253_L1.4331.00
259_V315_V1.4311.00
316_K320_E1.4211.00
305_A309_S1.4041.00
132_F143_V1.3961.00
8_T28_A1.3671.00
229_I253_L1.3661.00
63_V98_M1.3581.00
68_A72_N1.3521.00
68_A71_E1.3501.00
204_D227_K1.3351.00
159_T264_T1.3121.00
280_R284_Q1.3021.00
62_W94_A1.2971.00
103_K137_V1.2961.00
120_T123_K1.2951.00
27_K30_E1.2861.00
219_F249_K1.2861.00
138_G186_Q1.2701.00
139_G150_Q1.2661.00
263_T295_T1.2641.00
251_N285_D1.2601.00
49_L68_A1.2601.00
204_D217_E1.2451.00
252_E256_S1.2371.00
220_D223_K1.2371.00
158_R268_E1.2341.00
66_A70_K1.2291.00
124_K128_D1.2221.00
147_R163_D1.2161.00
47_Q72_N1.2151.00
67_E199_T1.2131.00
208_L219_F1.2021.00
176_I181_I1.1961.00
61_Y64_E1.1771.00
138_G183_Y1.1761.00
67_E71_E1.1671.00
219_F246_I1.1661.00
209_K295_T1.1570.99
259_V288_I1.1480.99
52_I57_M1.1340.99
148_V181_I1.1320.99
22_L114_P1.1230.99
8_T48_S1.1190.99
59_P62_W1.1120.99
138_G188_T1.1110.99
69_V79_F1.1090.99
221_A224_S1.1040.99
88_M110_G1.1000.99
65_I81_I1.0990.99
80_V130_I1.0980.99
4_L77_D1.0890.99
210_L240_P1.0890.99
153_R185_K1.0860.99
50_M65_I1.0800.99
149_I172_Y1.0660.99
250_V287_I1.0650.99
309_S318_I1.0650.99
205_V306_L1.0620.99
81_I108_I1.0580.99
45_E72_N1.0500.99
204_D226_Y1.0410.99
2_K136_G1.0350.99
79_F104_K1.0330.99
42_Y134_C1.0240.99
80_V134_C1.0190.99
222_L258_I1.0150.99
254_I260_V1.0110.99
302_L315_V1.0060.99
85_T110_G1.0000.98
70_K197_V0.9970.98
55_T238_G0.9950.98
141_Y173_I0.9940.98
3_K77_D0.9870.98
4_L134_C0.9750.98
110_G144_F0.9740.98
175_F184_N0.9730.98
281_R284_Q0.9710.98
203_T307_G0.9700.98
87_T234_Y0.9670.98
16_V30_E0.9660.98
309_S314_V0.9640.98
143_V176_I0.9590.98
299_V303_M0.9560.98
135_E183_Y0.9550.98
113_I162_Y0.9520.98
216_P252_E0.9430.98
89_A296_E0.9420.98
90_Y265_Q0.9350.98
42_Y130_I0.9330.98
265_Q296_E0.9280.98
231_I250_V0.9270.98
88_M108_I0.9260.97
85_T156_K0.9220.97
16_V25_G0.9200.97
254_I286_L0.9160.97
239_V250_V0.9160.97
13_I122_A0.9070.97
70_K74_D0.9030.97
7_L81_I0.8990.97
93_A97_Y0.8950.97
86_D235_G0.8920.97
155_I295_T0.8850.97
78_G105_P0.8840.97
210_L246_I0.8830.97
63_V67_E0.8820.97
36_S42_Y0.8820.97
60_E64_E0.8810.97
96_S151_G0.8810.97
250_V254_I0.8710.96
217_E221_A0.8650.96
36_S127_T0.8650.96
70_K199_T0.8640.96
222_L256_S0.8620.96
76_Y104_K0.8590.96
119_K124_K0.8590.96
158_R269_E0.8550.96
210_L231_I0.8520.96
160_K236_S0.8490.96
107_V141_Y0.8410.96
153_R297_A0.8390.96
238_G279_G0.8390.96
219_F250_V0.8380.95
89_A265_Q0.8340.95
6_M126_I0.8270.95
93_A152_T0.8260.95
310_S314_V0.8250.95
263_T298_I0.8240.95
69_V73_Y0.8240.95
55_T278_V0.8210.95
59_P212_P0.8190.95
132_F183_Y0.8170.95
254_I285_D0.8090.95
5_L47_Q0.8070.94
159_T270_G0.8010.94
222_L225_M0.8000.94
217_E224_S0.8000.94
14_A82_T0.7990.94
132_F176_I0.7990.94
102_A106_I0.7980.94
15_S24_P0.7970.94
56_N278_V0.7960.94
6_M82_T0.7940.94
210_L239_V0.7930.94
153_R292_N0.7930.94
223_K226_Y0.7910.94
147_R172_Y0.7900.94
5_L45_E0.7880.94
124_K162_Y0.7870.94
43_T77_D0.7870.94
96_S150_Q0.7870.94
205_V230_V0.7860.94
158_R161_S0.7850.94
159_T266_C0.7840.94
139_G173_I0.7840.94
314_V318_I0.7790.93
145_D163_D0.7770.93
13_I111_S0.7770.93
37_K40_N0.7750.93
298_I319_M0.7740.93
41_D134_C0.7730.93
41_D127_T0.7710.93
107_V133_A0.7700.93
295_T298_I0.7680.93
313_P317_R0.7640.93
155_I294_N0.7640.93
93_A300_P0.7610.93
156_K235_G0.7610.93
288_I319_M0.7590.92
48_S51_N0.7580.92
210_L234_Y0.7570.92
147_R166_E0.7510.92
157_L267_L0.7490.92
38_L130_I0.7480.92
7_L72_N0.7470.92
262_I273_M0.7450.92
53_D56_N0.7440.92
293_M301_K0.7430.92
248_S252_E0.7420.92
252_E255_E0.7410.92
100_Q196_T0.7400.92
86_D238_G0.7370.91
228_G298_I0.7350.91
76_Y79_F0.7350.91
121_D156_K0.7350.91
81_I95_L0.7340.91
73_Y99_L0.7330.91
115_I122_A0.7320.91
9_T83_H0.7280.91
69_V99_L0.7280.91
208_L218_M0.7260.91
208_L214_L0.7250.91
50_M64_E0.7230.91
184_N291_R0.7220.90
177_N236_S0.7220.90
37_K123_K0.7160.90
274_S279_G0.7160.90
141_Y148_V0.7150.90
214_L246_I0.7150.90
115_I120_T0.7150.90
66_A94_A0.7130.90
97_Y304_W0.7110.90
259_V312_L0.7100.90
254_I287_I0.7100.90
216_P219_F0.7070.90
13_I82_T0.7050.89
294_N297_A0.7040.89
59_P63_V0.7030.89
229_I250_V0.7030.89
282_V287_I0.7020.89
214_L219_F0.7010.89
9_T65_I0.7010.89
53_D278_V0.7000.89
55_T234_Y0.6980.89
168_I268_E0.6970.89
210_L214_L0.6930.89
97_Y301_K0.6920.89
17_E21_G0.6920.89
100_Q198_D0.6910.88
214_L240_P0.6900.88
234_Y240_P0.6870.88
109_T129_A0.6870.88
107_V132_F0.6870.88
6_M130_I0.6870.88
17_E116_T0.6830.88
198_D308_Q0.6830.88
254_I257_G0.6820.88
239_V246_I0.6810.88
26_V115_I0.6790.88
150_Q173_I0.6790.88
230_V261_V0.6790.88
89_A154_A0.6780.88
138_G141_Y0.6780.88
235_G238_G0.6770.87
141_Y183_Y0.6740.87
92_S142_V0.6740.87
62_W91_T0.6730.87
150_Q153_R0.6710.87
311_D318_I0.6690.87
174_A183_Y0.6680.87
112_Q164_A0.6670.87
203_T227_K0.6650.87
64_E68_A0.6620.86
311_D317_R0.6620.86
248_S251_N0.6610.86
212_P296_E0.6610.86
14_A28_A0.6590.86
181_I184_N0.6580.86
108_I151_G0.6540.86
155_I267_L0.6520.85
153_R301_K0.6500.85
304_W308_Q0.6500.85
66_A197_V0.6490.85
218_M226_Y0.6460.85
274_S283_N0.6450.85
222_L249_K0.6420.85
72_N76_Y0.6400.84
183_Y186_Q0.6390.84
2_K77_D0.6390.84
90_Y299_V0.6390.84
227_K258_I0.6360.84
63_V200_S0.6340.84
309_S312_L0.6280.83
70_K100_Q0.6270.83
5_L69_V0.6260.83
144_F149_I0.6240.83
97_Y297_A0.6230.83
114_P117_F0.6230.83
309_S315_V0.6220.83
297_A301_K0.6220.83
21_G24_P0.6200.82
110_G156_K0.6190.82
281_R285_D0.6190.82
112_Q156_K0.6180.82
92_S154_A0.6160.82
8_T15_S0.6130.82
93_A301_K0.6100.81
206_C215_K0.6090.81
97_Y152_T0.6090.81
29_D45_E0.6090.81
147_R175_F0.6070.81
132_F181_I0.6070.81
82_T126_I0.6070.81
35_V130_I0.6060.81
15_S22_L0.6060.81
188_T191_E0.6050.81
72_N79_F0.6030.81
7_L69_V0.6030.81
112_Q235_G0.5990.80
271_E289_R0.5980.80
67_E200_S0.5970.80
163_D166_E0.5970.80
236_S262_I0.5920.80
118_Q146_G0.5900.79
167_S171_P0.5890.79
75_A103_K0.5880.79
184_N189_E0.5870.79
141_Y174_A0.5860.79
129_A143_V0.5850.79
41_D130_I0.5850.79
93_A151_G0.5850.79
253_L258_I0.5840.79
216_P245_D0.5840.79
22_L117_F0.5830.78
14_A51_N0.5820.78
282_V285_D0.5820.78
128_D179_D0.5820.78
17_E22_L0.5820.78
228_G306_L0.5820.78
27_K115_I0.5800.78
67_E203_T0.5790.78
15_S55_T0.5780.78
4_L130_I0.5770.78
290_S298_I0.5750.78
231_I239_V0.5740.77
226_Y258_I0.5720.77
53_D88_M0.5720.77
219_F251_N0.5710.77
62_W95_L0.5710.77
64_E71_E0.5700.77
8_T46_T0.5690.77
61_Y65_I0.5680.77
54_S83_H0.5670.77
177_N180_G0.5670.77
306_L309_S0.5660.76
259_V306_L0.5660.76
63_V201_L0.5630.76
137_V141_Y0.5630.76
238_G282_V0.5620.76
228_G259_V0.5620.76
73_Y76_Y0.5620.76
233_S239_V0.5620.76
17_E117_F0.5610.76
155_I296_E0.5600.76
57_M91_T0.5600.76
290_S319_M0.5590.76
14_A31_L0.5590.76
66_A99_L0.5570.75
92_S108_I0.5570.75
53_D151_G0.5560.75
225_M256_S0.5530.75
186_Q190_P0.5510.75
144_F164_A0.5490.74
227_K310_S0.5480.74
131_R179_D0.5470.74
160_K270_G0.5470.74
174_A181_I0.5460.74
82_T139_G0.5460.74
30_E173_I0.5440.74
228_G263_T0.5440.74
222_L229_I0.5410.73
128_D176_I0.5410.73
85_T149_I0.5400.73
45_E77_D0.5380.73
112_Q144_F0.5380.73
36_S39_D0.5350.72
222_L226_Y0.5350.72
113_I119_K0.5330.72
60_E256_S0.5330.72
46_T49_L0.5330.72
4_L44_M0.5320.72
54_S237_G0.5300.72
132_F141_Y0.5300.72
315_V319_M0.5280.71
225_M232_E0.5280.71
253_L260_V0.5270.71
69_V95_L0.5270.71
209_K296_E0.5260.71
63_V94_A0.5260.71
129_A132_F0.5240.71
43_T248_S0.5230.71
49_L65_I0.5220.71
247_L251_N0.5220.71
207_L299_V0.5200.70
41_D44_M0.5190.70
38_L42_Y0.5180.70
249_K253_L0.5180.70
211_H303_M0.5180.70
305_A310_S0.5160.70
276_Y282_V0.5160.70
223_K255_E0.5160.70
22_L25_G0.5160.70
100_Q195_F0.5160.70
2_K42_Y0.5130.69
41_D131_R0.5110.69
169_N292_N0.5100.69
223_K252_E0.5100.69
63_V199_T0.5070.68
65_I68_A0.5070.68
6_M203_T0.5070.68
172_Y175_F0.5020.68
293_M297_A0.5010.67
155_I164_A0.5010.67
86_D276_Y0.5000.67
53_D238_G0.5000.67
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4q0mA 2 0.9909 100 0.032 Contact Map
1wlsA 2 0.9878 100 0.04 Contact Map
2d6fA 2 0.9878 100 0.048 Contact Map
1zq1A 2 0.9909 100 0.048 Contact Map
2himA 4 0.9666 100 0.059 Contact Map
4r8kA 3 0.9848 100 0.073 Contact Map
1nnsA 4 0.9574 100 0.095 Contact Map
1wsaA 4 0.9422 100 0.098 Contact Map
3nxkA 4 0.9514 100 0.1 Contact Map
1agxA 4 0.9544 100 0.1 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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