GREMLIN Database
ASPA - Aspartate ammonia-lyase
UniProt: P26899 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 475 (403)
Sequences: 10905 (5739)
Seq/√Len: 285.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
64_M74_G3.8771.00
73_I137_H3.4661.00
65_D252_H3.3241.00
57_K82_D3.3071.00
61_L78_V3.2371.00
400_R403_Q3.2061.00
51_A158_K2.8771.00
168_E215_I2.8401.00
61_L256_I2.7441.00
288_M343_V2.7281.00
171_H212_E2.7171.00
164_L219_K2.6201.00
402_K406_E2.6171.00
160_L280_V2.6081.00
173_V392_K2.6071.00
47_E377_S2.5951.00
117_R121_I2.5391.00
161_E222_R2.5171.00
449_T452_E2.5131.00
346_N375_S2.4861.00
224_H279_E2.4821.00
200_L396_A2.4771.00
252_H255_D2.4191.00
164_L215_I2.3901.00
339_I382_G2.2951.00
177_K180_E2.2931.00
285_K347_D2.2571.00
70_Y73_I2.2421.00
157_L226_Y2.2271.00
183_S398_E2.1981.00
64_M71_E2.1921.00
226_Y260_P2.1531.00
313_S395_E2.0701.00
168_E219_K2.0551.00
403_Q407_K2.0381.00
166_T384_R2.0051.00
342_Q371_N2.0051.00
171_H215_I1.9981.00
71_E75_Q1.9661.00
176_Q392_K1.9641.00
173_V387_T1.9511.00
436_Q444_Q1.9461.00
176_Q180_E1.9361.00
452_E455_I1.9241.00
252_H256_I1.9221.00
400_R404_Y1.9071.00
173_V391_L1.8911.00
289_M344_I1.8901.00
220_Q224_H1.8811.00
295_A336_I1.8721.00
215_I219_K1.8591.00
415_V418_H1.8301.00
54_I158_K1.8191.00
75_Q79_Q1.8061.00
217_R279_E1.7921.00
83_E121_I1.7511.00
167_M287_C1.7461.00
175_K212_E1.7401.00
402_K460_Y1.7351.00
57_K78_V1.7331.00
228_V254_A1.7311.00
220_Q223_Q1.7311.00
181_F184_V1.7061.00
423_A426_R1.7041.00
387_T392_K1.7011.00
450_E454_D1.6991.00
291_M336_I1.6881.00
171_H208_S1.6611.00
446_D449_T1.6551.00
398_E402_K1.6521.00
439_R454_D1.6501.00
458_N461_E1.6491.00
436_Q441_L1.6421.00
278_T354_S1.6311.00
214_D290_N1.6271.00
61_L252_H1.6181.00
264_A270_A1.6171.00
74_G78_V1.6101.00
227_E262_V1.6101.00
213_R269_D1.6061.00
169_D387_T1.5751.00
230_M249_V1.5561.00
251_K255_D1.5541.00
76_A122_M1.5421.00
443_L450_E1.5301.00
57_K85_L1.5221.00
166_T380_N1.5201.00
232_A270_A1.4951.00
349_T367_V1.4941.00
430_E445_H1.4751.00
177_K181_F1.4741.00
178_A208_S1.4691.00
348_N352_L1.4381.00
62_A252_H1.4371.00
430_E434_T1.4331.00
223_Q260_P1.4291.00
285_K351_C1.4151.00
221_S283_S1.4071.00
159_L380_N1.3881.00
213_R275_D1.3781.00
117_R120_E1.3781.00
333_A390_C1.3741.00
274_T365_E1.3561.00
82_D86_E1.3461.00
281_S350_I1.3431.00
216_K224_H1.3351.00
87_G121_I1.3271.00
50_N54_I1.3211.00
397_N400_R1.3201.00
399_K403_Q1.3161.00
300_L358_Q1.3111.00
171_H211_L1.3071.00
47_E159_L1.3061.00
57_K61_L1.3031.00
295_A386_F1.2961.00
156_T280_V1.2931.00
282_A286_V1.2881.00
451_E454_D1.2801.00
76_A79_Q1.2751.00
204_F207_Y1.2751.00
289_M354_S1.2671.00
52_L147_V1.2661.00
278_T286_V1.2651.00
60_A69_L1.2581.00
164_L222_R1.2581.00
169_D392_K1.2571.00
175_K179_Q1.2541.00
42_Y374_Q1.2461.00
50_N85_L1.2411.00
342_Q374_Q1.2381.00
207_Y360_E1.2361.00
423_A447_V1.2341.00
437_S440_D1.2311.00
173_V176_Q1.2301.00
344_I352_L1.2281.00
110_A114_I1.2261.00
419_L427_I1.2171.00
184_V398_E1.2141.00
311_E397_N1.2131.00
200_L204_F1.2121.00
345_G349_T1.2121.00
167_M211_L1.2081.00
311_E395_E1.2081.00
436_Q440_D1.2001.00
141_S241_A1.1971.00
179_Q182_D1.1971.00
53_A85_L1.1941.00
181_F398_E1.1761.00
53_A110_A1.1721.00
218_I287_C1.1621.00
68_R140_M1.1611.00
53_A84_I1.1591.00
156_T372_L1.1521.00
254_A260_P1.1491.00
166_T387_T1.1461.00
71_E74_G1.1461.00
350_I372_L1.1451.00
83_E117_R1.1421.00
445_H449_T1.1411.00
336_I382_G1.1391.00
194_D305_P1.1281.00
175_K208_S1.1231.00
83_E88_K1.1231.00
200_L401_M1.1141.00
168_E172_S1.1131.00
141_S242_D1.1061.00
182_D201_G1.1061.00
347_D351_C1.0961.00
166_T169_D1.0941.00
164_L168_E1.0941.00
174_F294_I1.0921.00
298_L333_A1.0861.00
250_V254_A1.0841.00
417_P452_E1.0811.00
80_A83_E1.0811.00
186_K401_M1.0791.00
170_M211_L1.0761.00
82_D85_L1.0741.00
157_L259_L1.0741.00
340_A344_I1.0721.00
169_D384_R1.0711.00
159_L162_K1.0651.00
50_N89_W1.0631.00
58_A256_I1.0601.00
209_R265_D1.0591.00
76_A80_A1.0581.00
183_S201_G1.0551.00
277_Y372_L1.0481.00
197_P405_V1.0481.00
439_R443_L1.0481.00
170_M391_L1.0471.00
434_T441_L1.0421.00
162_K380_N1.0411.00
207_Y294_I1.0401.00
216_K223_Q1.0381.00
51_A154_I1.0351.00
55_V147_V1.0311.00
46_E90_H1.0281.00
445_H448_L1.0261.00
150_T228_V1.0241.00
75_Q78_V1.0231.00
283_S287_C1.0231.00
194_D304_G1.0131.00
152_I369_V1.0121.00
182_D199_R1.0101.00
418_H456_I1.0091.00
226_Y259_L1.0091.00
177_K204_F1.0081.00
184_V401_M1.0081.00
160_L222_R1.0071.00
78_V82_D1.0061.00
292_S337_N1.0041.00
177_K391_L1.0031.00
198_I235_V0.9961.00
198_I268_V0.9941.00
79_Q121_I0.9921.00
199_R463_T0.9921.00
65_D256_I0.9901.00
440_D444_Q0.9881.00
50_N158_K0.9881.00
191_H334_E0.9851.00
64_M75_Q0.9801.00
419_L424_A0.9801.00
94_I119_L0.9781.00
157_L222_R0.9771.00
300_L359_L0.9771.00
317_R389_N0.9741.00
415_V419_L0.9741.00
313_S397_N0.9741.00
152_I277_Y0.9721.00
83_E87_G0.9711.00
228_V253_L0.9701.00
54_I256_I0.9681.00
188_G198_I0.9671.00
209_R213_R0.9651.00
291_M383_F0.9651.00
167_M215_I0.9641.00
222_R226_Y0.9631.00
280_V372_L0.9631.00
298_L394_I0.9581.00
185_I463_T0.9551.00
437_S441_L0.9541.00
76_A134_P0.9461.00
220_Q279_E0.9421.00
160_L218_I0.9421.00
46_E50_N0.9411.00
94_I97_P0.9351.00
216_K220_Q0.9321.00
79_Q83_E0.9271.00
54_I253_L0.9261.00
52_L110_A0.9241.00
438_V441_L0.9241.00
108_M111_N0.9231.00
202_Q267_L0.9231.00
189_R194_D0.9181.00
66_V242_D0.9131.00
55_V150_T0.9091.00
312_I394_I0.9091.00
282_A347_D0.9071.00
47_E158_K0.8991.00
171_H175_K0.8981.00
111_N115_G0.8971.00
172_S176_Q0.8941.00
281_S347_D0.8931.00
262_V265_D0.8881.00
342_Q370_F0.8871.00
174_F204_F0.8861.00
70_Y137_H0.8861.00
54_I85_L0.8851.00
52_L55_V0.8831.00
406_E439_R0.8791.00
62_A66_V0.8781.00
430_E444_Q0.8771.00
72_G75_Q0.8741.00
180_E395_E0.8711.00
281_S372_L0.8701.00
178_A205_E0.8691.00
405_V459_P0.8691.00
184_V396_A0.8681.00
189_R305_P0.8681.00
62_A249_V0.8631.00
287_C383_F0.8621.00
208_S212_E0.8611.00
451_E455_I0.8591.00
93_F97_P0.8591.00
51_A155_S0.8551.00
430_E441_L0.8521.00
418_H452_E0.8491.00
439_R450_E0.8481.00
268_V360_E0.8461.00
441_L444_Q0.8451.00
203_E360_E0.8431.00
158_K161_E0.8351.00
192_L300_L0.8351.00
133_S136_T0.8331.00
152_I372_L0.8271.00
370_F374_Q0.8241.00
292_S355_E0.8231.00
440_D450_E0.8201.00
452_E456_I0.8120.99
336_I386_F0.8120.99
194_D408_S0.8110.99
119_L135_N0.8110.99
263_G267_L0.8090.99
174_F208_S0.8050.99
174_F391_L0.8050.99
289_M351_C0.8040.99
249_V253_L0.8030.99
273_N290_N0.8020.99
86_E90_H0.8020.99
282_A351_C0.8000.99
173_V177_K0.7990.99
185_I405_V0.7940.99
312_I393_G0.7910.99
64_M69_L0.7900.99
181_F396_A0.7900.99
294_I386_F0.7890.99
83_E89_W0.7890.99
50_N90_H0.7840.99
65_D71_E0.7830.99
341_F349_T0.7820.99
87_G117_R0.7810.99
346_N374_Q0.7810.99
203_E301_M0.7800.99
89_W117_R0.7790.99
279_E282_A0.7780.99
349_T371_N0.7740.99
59_A142_Q0.7730.99
233_T323_I0.7720.99
60_A74_G0.7710.99
192_L303_S0.7710.99
227_E276_A0.7700.99
115_G118_A0.7660.99
213_R227_E0.7660.99
73_I134_P0.7660.99
187_M307_A0.7610.99
180_E398_E0.7600.99
342_Q346_N0.7580.99
72_G76_A0.7550.99
229_N270_A0.7550.99
313_S393_G0.7530.99
426_R430_E0.7480.99
344_I348_N0.7450.99
416_N419_L0.7450.99
226_Y254_A0.7440.99
80_A122_M0.7440.99
167_M383_F0.7430.99
308_G397_N0.7430.99
183_S460_Y0.7410.99
301_M359_L0.7400.99
453_L457_L0.7390.99
186_K203_E0.7360.99
250_V263_G0.7300.99
313_S316_A0.7290.99
57_K256_I0.7260.99
58_A61_L0.7250.99
189_R304_G0.7240.99
275_D290_N0.7240.99
390_C394_I0.7240.99
443_L454_D0.7220.99
160_L221_S0.7220.99
73_I77_I0.7220.99
272_Q360_E0.7220.99
341_F353_A0.7200.99
77_I134_P0.7200.99
69_L140_M0.7200.99
353_A367_V0.7190.99
61_L65_D0.7190.99
269_D275_D0.7190.99
436_Q442_C0.7190.99
448_L456_I0.7150.99
269_D290_N0.7130.99
280_V284_L0.7110.99
295_A333_A0.7080.99
81_A84_I0.7080.99
250_V253_L0.7070.99
44_I370_F0.7070.99
184_V199_R0.7060.99
211_L383_F0.7060.99
48_M148_F0.7040.99
315_P393_G0.7020.99
280_V377_S0.7000.99
87_G92_Q0.7000.99
116_N120_E0.6990.98
85_L89_W0.6980.98
163_L380_N0.6960.98
206_A293_K0.6940.98
277_Y350_I0.6930.98
170_M387_T0.6930.98
294_I391_L0.6920.98
399_K402_K0.6920.98
162_K166_T0.6900.98
176_Q179_Q0.6900.98
65_D242_D0.6880.98
84_I114_I0.6880.98
396_A401_M0.6880.98
163_L376_I0.6880.98
346_N376_I0.6870.98
153_H276_A0.6860.98
217_R275_D0.6850.98
172_S175_K0.6850.98
57_K74_G0.6820.98
210_V290_N0.6800.98
168_E212_E0.6790.98
210_V273_N0.6790.98
342_Q378_I0.6750.98
68_R242_D0.6750.98
134_P137_H0.6730.98
211_L214_D0.6730.98
296_N337_N0.6690.98
419_L423_A0.6680.98
278_T350_I0.6660.98
204_F401_M0.6660.98
253_L256_I0.6650.98
223_Q226_Y0.6640.98
214_D286_V0.6640.98
230_M253_L0.6630.98
60_A77_I0.6630.98
157_L161_E0.6620.98
184_V402_K0.6610.98
287_C379_M0.6600.98
174_F211_L0.6600.98
344_I351_C0.6540.98
181_F200_L0.6540.98
168_E171_H0.6540.98
216_K219_K0.6530.98
73_I76_A0.6520.98
460_Y464_K0.6510.98
383_F387_T0.6480.98
372_L376_I0.6460.97
161_E259_L0.6460.97
157_L260_P0.6440.97
66_V252_H0.6430.97
304_G308_G0.6420.97
169_D173_V0.6420.97
160_L225_L0.6420.97
227_E264_A0.6420.97
155_S376_I0.6400.97
42_Y370_F0.6400.97
187_M405_V0.6400.97
186_K198_I0.6370.97
348_N351_C0.6360.97
249_V252_H0.6360.97
278_T282_A0.6350.97
170_M383_F0.6350.97
133_S422_E0.6320.97
448_L452_E0.6320.97
310_A397_N0.6310.97
400_R407_K0.6310.97
292_S352_L0.6300.97
341_F352_L0.6280.97
163_L284_L0.6240.97
221_S225_L0.6240.97
210_V272_Q0.6230.97
84_I90_H0.6230.97
217_R220_Q0.6220.97
61_L74_G0.6220.97
159_L376_I0.6210.97
336_I390_C0.6200.97
60_A138_V0.6170.97
455_I461_E0.6170.97
137_H140_M0.6160.97
81_A114_I0.6140.97
308_G400_R0.6130.97
68_R141_S0.6130.97
160_L164_L0.6110.97
249_V254_A0.6070.96
64_M78_V0.6050.96
349_T368_L0.6040.96
216_K227_E0.6030.96
438_V442_C0.6020.96
285_K289_M0.6020.96
332_M386_F0.6020.96
291_M387_T0.6020.96
178_A182_D0.6010.96
217_R224_H0.6000.96
404_Y407_K0.6000.96
405_V410_G0.5990.96
112_E116_N0.5990.96
278_T351_C0.5980.96
214_D217_R0.5960.96
345_G348_N0.5960.96
69_L74_G0.5950.96
349_T353_A0.5950.96
280_V376_I0.5950.96
165_K169_D0.5950.96
69_L77_I0.5940.96
207_Y210_V0.5940.96
317_R393_G0.5930.96
221_S280_V0.5900.96
420_G423_A0.5900.96
406_E437_S0.5900.96
308_G312_I0.5890.96
179_Q183_S0.5880.96
185_I459_P0.5880.96
115_G119_L0.5870.96
337_N364_M0.5870.96
164_L283_S0.5860.96
53_A81_A0.5860.96
69_L73_I0.5850.96
108_M112_E0.5840.95
70_Y74_G0.5830.95
163_L379_M0.5810.95
284_L379_M0.5810.95
177_K394_I0.5800.95
218_I283_S0.5790.95
175_K178_A0.5790.95
425_A429_R0.5780.95
455_I458_N0.5770.95
430_E436_Q0.5770.95
288_M379_M0.5770.95
284_L287_C0.5770.95
279_E286_V0.5750.95
398_E401_M0.5750.95
461_E464_K0.5740.95
441_L445_H0.5730.95
109_N115_G0.5720.95
203_E297_D0.5710.95
55_V151_A0.5650.95
53_A90_H0.5630.95
109_N114_I0.5630.95
282_A285_K0.5630.95
332_M390_C0.5610.94
211_L287_C0.5610.94
86_E89_W0.5610.94
60_A78_V0.5590.94
47_E162_K0.5570.94
156_T225_L0.5570.94
369_V373_L0.5570.94
371_N374_Q0.5570.94
116_N123_G0.5560.94
167_M218_I0.5550.94
49_I88_K0.5550.94
212_E216_K0.5520.94
448_L453_L0.5500.94
211_L291_M0.5500.94
54_I58_A0.5500.94
295_A337_N0.5490.94
194_D410_G0.5490.94
217_R286_V0.5490.94
339_I378_I0.5490.94
51_A54_I0.5480.94
53_A87_G0.5480.94
144_T229_N0.5470.94
170_M173_V0.5470.94
292_S356_A0.5470.94
234_A323_I0.5470.94
286_V354_S0.5460.94
440_D443_L0.5450.94
223_Q227_E0.5450.94
204_F394_I0.5420.93
108_M115_G0.5410.93
443_L453_L0.5410.93
199_R464_K0.5400.93
191_H363_V0.5380.93
391_L394_I0.5370.93
119_L123_G0.5370.93
55_V230_M0.5370.93
445_H453_L0.5360.93
453_L456_I0.5360.93
209_R212_E0.5350.93
229_N271_T0.5350.93
77_I122_M0.5340.93
426_R445_H0.5330.93
122_M134_P0.5330.93
298_L391_L0.5310.93
186_K301_M0.5310.93
111_N114_I0.5300.93
93_F113_V0.5290.93
254_A257_S0.5280.92
184_V201_G0.5280.92
281_S375_S0.5270.92
54_I257_S0.5270.92
221_S279_E0.5260.92
192_L358_Q0.5260.92
107_N315_P0.5240.92
300_L303_S0.5220.92
89_W92_Q0.5210.92
59_A253_L0.5180.92
219_K223_Q0.5180.92
354_S365_E0.5170.92
157_L225_L0.5160.92
198_I202_Q0.5160.92
244_E255_D0.5150.92
205_E208_S0.5140.91
286_V290_N0.5120.91
298_L386_F0.5110.91
164_L171_H0.5100.91
195_A235_V0.5100.91
213_R224_H0.5080.91
275_D279_E0.5080.91
318_Q331_V0.5080.91
432_I435_G0.5070.91
412_I424_A0.5060.91
142_Q147_V0.5050.91
228_V263_G0.5040.91
395_E398_E0.5040.91
166_T385_S0.5030.91
164_L167_M0.5020.90
254_A259_L0.5020.90
449_T455_I0.5020.90
233_T241_A0.5010.90
336_I340_A0.5000.90
74_G77_I0.5000.90
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1furA 4 0.9642 100 0.164 Contact Map
1yfmA 4 0.9411 100 0.165 Contact Map
1vdkA 4 0.9621 100 0.169 Contact Map
3r6qA 4 0.9747 100 0.173 Contact Map
3gtdA 4 0.9558 100 0.178 Contact Map
4hgvA 4 0.9684 100 0.178 Contact Map
3e04A 4 0.9663 100 0.18 Contact Map
1jswA 4 0.9621 100 0.181 Contact Map
3ocfA 4 0.9411 100 0.192 Contact Map
4admA 4 0.9537 100 0.194 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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