SACT - BACSU - GREMLIN Contacts

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SACT - SacPA operon antiterminator

UniProt:	P26212	Sequences: MKIYKVLNNNAALIKEDDQEKIVMGPGIAFQKKKNDLIPMNKVEKIFVVRDENEKFKQILQTLPEEHIEIAEDIISYAEGELAAPLSDHIHIALSDHLSFAIERIQNGLLVQNKLLHEIKALYKKEYEIGLWAIGHVKETLGVSLPEDEAGYIALHIHTAKMDAESMYSALKHTTMIKEMIEKIKQYFNRKVDENSISYQRLVTHLRYAVSRLESNEALHRMDEEMLYFIQKKYSFAYQCALELAEFLKNEYQLHLPESEAGYITLHVQRLQDLSE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	276 (264)
Sequences:	3643 (3156)
Seq/√Len:	194.3

ECOLI - BGLG CSIE FRVR
BACSU - SACT LICT SACY GLCT MANR MTLR LICR

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
138_K	144_S	4.614	1.00
81_E	132_W	4.275	1.00
249_K	255_H	3.051	1.00
227_L	262_G	2.969	1.00
185_K	191_K	2.921	1.00
187_Y	243_E	2.916	1.00
231_Q	238_Y	2.810	1.00
231_Q	258_E	2.777	1.00
237_A	269_Q	2.760	1.00
103_E	107_N	2.747	1.00
179_E	251_E	2.517	1.00
73_D	140_T	2.491	1.00
37_L	41_N	2.472	1.00
241_A	265_T	2.438	1.00
28_I	33_K	2.330	1.00
131_L	150_A	2.262	1.00
184_I	202_L	2.235	1.00
74_I	133_A	2.151	1.00
97_H	156_H	2.115	1.00
26_P	30_F	2.080	1.00
27_G	30_F	2.078	1.00
7_L	13_L	2.059	1.00
30_F	33_K	1.995	1.00
183_K	247_F	1.991	1.00
216_N	254_L	1.990	1.00
77_Y	136_H	1.985	1.00
241_A	261_A	1.962	1.00
37_L	40_M	1.958	1.00
11_A	15_K	1.955	1.00
212_R	260_E	1.912	1.00
39_P	42_K	1.893	1.00
245_A	261_A	1.822	1.00
237_A	241_A	1.797	1.00
242_L	258_E	1.787	1.00
178_K	199_Y	1.765	1.00
128_E	131_L	1.762	1.00
77_Y	132_W	1.734	1.00
184_I	268_V	1.726	1.00
14_I	21_K	1.693	1.00
78_A	133_A	1.676	1.00
131_L	147_E	1.653	1.00
195_N	271_L	1.652	1.00
188_F	240_C	1.595	1.00
237_A	268_V	1.576	1.00
105_I	145_L	1.556	1.00
116_L	151_G	1.545	1.00
19_Q	23_V	1.521	1.00
7_L	11_A	1.519	1.00
19_Q	30_F	1.515	1.00
184_I	240_C	1.507	1.00
182_E	186_Q	1.504	1.00
180_M	244_L	1.502	1.00
11_A	16_E	1.493	1.00
41_N	44_E	1.488	1.00
239_Q	242_L	1.486	1.00
12_A	15_K	1.485	1.00
134_I	138_K	1.465	1.00
93_A	156_H	1.465	1.00
20_E	27_G	1.464	1.00
105_I	146_P	1.459	1.00
229_F	233_K	1.455	1.00
27_G	31_Q	1.449	1.00
242_L	261_A	1.401	1.00
11_A	14_I	1.396	1.00
245_A	264_I	1.388	1.00
246_E	249_K	1.378	1.00
205_H	267_H	1.376	1.00
7_L	15_K	1.374	1.00
210_V	214_E	1.372	1.00
224_E	262_G	1.365	1.00
181_I	199_Y	1.354	1.00
19_Q	28_I	1.354	1.00
64_P	67_H	1.353	1.00
230_I	265_T	1.352	1.00
78_A	129_I	1.350	1.00
26_P	33_K	1.348	1.00
36_D	40_M	1.347	1.00
10_N	15_K	1.336	1.00
134_I	150_A	1.289	1.00
16_E	19_Q	1.279	1.00
198_S	271_L	1.275	1.00
79_E	86_L	1.267	1.00
241_A	245_A	1.257	1.00
70_I	140_T	1.233	1.00
21_K	30_F	1.230	1.00
8_N	16_E	1.225	1.00
89_H	93_A	1.221	1.00
130_G	150_A	1.219	1.00
8_N	11_A	1.217	1.00
209_A	213_L	1.198	1.00
219_L	263_Y	1.187	1.00
20_E	28_I	1.182	1.00
8_N	15_K	1.173	1.00
19_Q	33_K	1.172	1.00
192_V	268_V	1.170	1.00
75_I	95_S	1.167	1.00
102_I	106_Q	1.163	1.00
126_E	154_A	1.162	1.00
220_H	259_S	1.159	1.00
45_K	49_V	1.153	1.00
40_M	44_E	1.142	1.00
10_N	14_I	1.129	1.00
259_S	262_G	1.123	1.00
184_I	241_A	1.119	1.00
93_A	97_H	1.117	1.00
67_H	71_A	1.114	1.00
175_T	251_E	1.107	1.00
74_I	78_A	1.106	1.00
19_Q	26_P	1.097	1.00
227_L	258_E	1.096	1.00
206_L	209_A	1.090	1.00
209_A	257_P	1.087	1.00
225_E	259_S	1.086	1.00
67_H	99_S	1.084	1.00
146_P	149_E	1.084	1.00
71_A	75_I	1.081	1.00
38_I	41_N	1.061	1.00
102_I	145_L	1.058	1.00
37_L	44_E	1.051	1.00
228_Y	232_K	1.047	1.00
99_S	103_E	1.045	1.00
26_P	29_A	1.038	1.00
183_K	186_Q	1.025	1.00
173_H	210_V	1.022	1.00
209_A	256_L	1.021	1.00
70_I	141_L	1.013	1.00
23_V	26_P	1.013	1.00
241_A	268_V	1.012	1.00
224_E	259_S	1.011	1.00
34_K	37_L	1.009	1.00
37_L	42_K	1.007	1.00
238_Y	265_T	1.005	1.00
130_G	154_A	0.997	1.00
242_L	246_E	0.982	0.99
16_E	21_K	0.982	0.99
192_V	271_L	0.982	0.99
127_Y	150_A	0.969	0.99
74_I	136_H	0.968	0.99
98_L	153_I	0.967	0.99
77_Y	81_E	0.966	0.99
157_I	161_K	0.964	0.99
10_N	13_L	0.964	0.99
241_A	264_I	0.958	0.99
40_M	46_I	0.954	0.99
21_K	26_P	0.951	0.99
44_E	49_V	0.949	0.99
241_A	244_L	0.949	0.99
46_I	49_V	0.945	0.99
43_V	47_F	0.940	0.99
238_Y	258_E	0.939	0.99
67_H	70_I	0.938	0.99
124_K	127_Y	0.938	0.99
130_G	133_A	0.935	0.99
135_G	144_S	0.935	0.99
177_I	203_V	0.935	0.99
138_K	141_L	0.933	0.99
20_E	32_K	0.930	0.99
20_E	33_K	0.929	0.99
177_I	180_M	0.927	0.99
244_L	268_V	0.922	0.99
79_E	82_L	0.919	0.99
8_N	14_I	0.915	0.99
12_A	29_A	0.914	0.99
238_Y	242_L	0.911	0.99
248_L	252_Y	0.907	0.99
127_Y	154_A	0.905	0.99
72_E	92_I	0.902	0.99
98_L	137_V	0.900	0.99
199_Y	203_V	0.897	0.99
91_H	95_S	0.896	0.99
127_Y	131_L	0.895	0.99
78_A	157_I	0.891	0.99
90_I	160_A	0.890	0.99
227_L	238_Y	0.889	0.99
209_A	212_R	0.888	0.99
262_G	265_T	0.887	0.99
179_E	247_F	0.887	0.99
110_L	146_P	0.877	0.99
154_A	158_H	0.877	0.99
137_V	145_L	0.873	0.99
70_I	74_I	0.872	0.99
26_P	31_Q	0.867	0.99
240_C	244_L	0.865	0.99
25_G	31_Q	0.861	0.99
230_I	262_G	0.858	0.99
16_E	20_E	0.855	0.99
72_E	91_H	0.849	0.98
12_A	23_V	0.846	0.98
76_S	91_H	0.844	0.98
82_L	132_W	0.844	0.98
46_I	50_R	0.836	0.98
71_A	98_L	0.836	0.98
134_I	153_I	0.834	0.98
132_W	135_G	0.834	0.98
244_L	247_F	0.833	0.98
27_G	33_K	0.829	0.98
46_I	53_N	0.829	0.98
67_H	102_I	0.828	0.98
269_Q	272_Q	0.826	0.98
133_A	136_H	0.826	0.98
138_K	145_L	0.817	0.98
40_M	43_V	0.815	0.98
210_V	252_Y	0.814	0.98
185_K	192_V	0.811	0.98
188_F	192_V	0.808	0.98
245_A	256_L	0.808	0.98
185_K	195_N	0.804	0.98
70_I	136_H	0.804	0.98
69_E	72_E	0.803	0.98
132_W	136_H	0.801	0.98
20_E	30_F	0.800	0.98
15_K	18_D	0.800	0.98
187_Y	240_C	0.796	0.98
127_Y	151_G	0.794	0.98
201_R	270_R	0.793	0.98
131_L	135_G	0.793	0.98
267_H	270_R	0.790	0.98
204_T	208_Y	0.789	0.98
116_L	152_Y	0.786	0.97
183_K	187_Y	0.785	0.97
263_Y	267_H	0.785	0.97
180_M	247_F	0.784	0.97
36_D	41_N	0.783	0.97
12_A	38_I	0.779	0.97
246_E	250_N	0.779	0.97
247_F	251_E	0.777	0.97
21_K	33_K	0.776	0.97
237_A	272_Q	0.775	0.97
213_L	254_L	0.775	0.97
184_I	188_F	0.774	0.97
69_E	73_D	0.773	0.97
237_A	265_T	0.771	0.97
113_N	119_I	0.769	0.97
113_N	151_G	0.764	0.97
84_A	266_L	0.759	0.97
225_E	229_F	0.759	0.97
202_L	264_I	0.759	0.97
122_L	147_E	0.757	0.97
160_A	209_A	0.747	0.97
31_Q	37_L	0.741	0.96
180_M	268_V	0.741	0.96
106_Q	142_G	0.740	0.96
208_Y	211_S	0.737	0.96
14_I	23_V	0.733	0.96
176_M	180_M	0.730	0.96
179_E	182_E	0.729	0.96
225_E	233_K	0.726	0.96
177_I	206_L	0.718	0.96
170_A	174_T	0.713	0.96
238_Y	241_A	0.707	0.95
86_L	157_I	0.706	0.95
197_I	201_R	0.703	0.95
41_N	45_K	0.702	0.95
114_K	155_L	0.701	0.95
23_V	30_F	0.700	0.95
134_I	137_V	0.699	0.95
84_A	161_K	0.695	0.95
80_G	83_A	0.692	0.95
101_A	105_I	0.690	0.95
72_E	76_S	0.689	0.95
20_E	26_P	0.689	0.95
97_H	101_A	0.688	0.95
54_E	57_K	0.688	0.95
39_P	43_V	0.688	0.95
31_Q	35_N	0.688	0.95
37_L	43_V	0.686	0.94
104_R	152_Y	0.685	0.94
125_K	128_E	0.684	0.94
149_E	263_Y	0.684	0.94
236_F	241_A	0.683	0.94
44_E	47_F	0.679	0.94
119_I	151_G	0.677	0.94
28_I	31_Q	0.677	0.94
201_R	267_H	0.676	0.94
9_N	15_K	0.675	0.94
201_R	271_L	0.671	0.94
207_R	211_S	0.669	0.94
177_I	181_I	0.668	0.94
7_L	12_A	0.664	0.93
167_M	170_A	0.664	0.93
174_T	203_V	0.658	0.93
24_M	30_F	0.654	0.93
20_E	35_N	0.654	0.93
11_A	24_M	0.654	0.93
198_S	202_L	0.652	0.93
192_V	198_S	0.652	0.93
251_E	254_L	0.649	0.93
63_L	99_S	0.648	0.93
20_E	31_Q	0.647	0.93
22_I	27_G	0.647	0.93
227_L	231_Q	0.645	0.92
33_K	37_L	0.644	0.92
20_E	23_V	0.644	0.92
248_L	254_L	0.642	0.92
30_F	37_L	0.641	0.92
7_L	16_E	0.641	0.92
78_A	132_W	0.641	0.92
136_H	139_E	0.640	0.92
21_K	25_G	0.638	0.92
78_A	136_H	0.638	0.92
102_I	137_V	0.636	0.92
86_L	90_I	0.636	0.92
67_H	106_Q	0.635	0.92
225_E	262_G	0.634	0.92
12_A	33_K	0.623	0.91
178_K	182_E	0.621	0.91
105_I	110_L	0.621	0.91
239_Q	243_E	0.619	0.91
236_F	239_Q	0.618	0.91
103_E	106_Q	0.618	0.91
176_M	206_L	0.612	0.90
9_N	16_E	0.611	0.90
31_Q	34_K	0.611	0.90
76_S	79_E	0.611	0.90
77_Y	129_I	0.611	0.90
182_E	193_D	0.610	0.90
105_I	149_E	0.607	0.90
229_F	232_K	0.607	0.90
169_S	172_K	0.605	0.90
113_N	148_D	0.605	0.90
82_L	86_L	0.604	0.90
183_K	244_L	0.602	0.90
12_A	28_I	0.602	0.90
19_Q	22_I	0.596	0.89
183_K	243_E	0.596	0.89
182_E	185_K	0.595	0.89
73_D	77_Y	0.593	0.89
89_H	160_A	0.592	0.89
58_Q	61_Q	0.592	0.89
219_L	259_S	0.592	0.89
43_V	46_I	0.591	0.89
173_H	203_V	0.588	0.89
188_F	191_K	0.587	0.89
241_A	254_L	0.587	0.89
133_A	157_I	0.586	0.88
113_N	118_E	0.585	0.88
188_F	244_L	0.583	0.88
269_Q	273_D	0.582	0.88
71_A	95_S	0.581	0.88
82_L	126_E	0.580	0.88
234_Y	269_Q	0.579	0.88
76_S	80_G	0.578	0.88
136_H	140_T	0.577	0.88
175_T	179_E	0.571	0.87
137_V	141_L	0.569	0.87
117_H	121_A	0.569	0.87
238_Y	261_A	0.566	0.87
205_H	263_Y	0.566	0.87
15_K	21_K	0.566	0.87
127_Y	147_E	0.565	0.87
22_I	30_F	0.564	0.86
242_L	245_A	0.563	0.86
148_D	151_G	0.560	0.86
66_E	140_T	0.560	0.86
153_I	156_H	0.554	0.86
38_I	45_K	0.552	0.85
10_N	30_F	0.547	0.85
14_I	22_I	0.545	0.85
65_E	69_E	0.544	0.84
231_Q	262_G	0.541	0.84
229_F	234_Y	0.541	0.84
25_G	30_F	0.538	0.84
131_L	144_S	0.537	0.84
176_M	252_Y	0.537	0.84
227_L	259_S	0.534	0.83
127_Y	158_H	0.534	0.83
224_E	228_Y	0.533	0.83
75_I	133_A	0.531	0.83
181_I	185_K	0.529	0.83
81_E	125_K	0.528	0.83
180_M	241_A	0.528	0.83
181_I	268_V	0.526	0.82
213_L	257_P	0.521	0.82
195_N	273_D	0.521	0.82
124_K	154_A	0.519	0.82
204_T	259_S	0.517	0.81
126_E	155_L	0.516	0.81
249_K	252_Y	0.514	0.81
106_Q	135_G	0.513	0.81
248_L	256_L	0.512	0.81
82_L	129_I	0.511	0.81
27_G	39_P	0.511	0.81
12_A	21_K	0.511	0.81
21_K	28_I	0.511	0.81
27_G	35_N	0.510	0.81
74_I	77_Y	0.509	0.80
104_R	149_E	0.507	0.80
102_I	143_V	0.507	0.80
173_H	177_I	0.507	0.80
90_I	209_A	0.506	0.80
12_A	30_F	0.504	0.80
174_T	199_Y	0.504	0.80
130_G	157_I	0.503	0.80
212_R	219_L	0.503	0.80
28_I	35_N	0.500	0.79
240_C	268_V	0.500	0.79

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4r6iA	2	0.9601	100	0.332	Contact Map
1h99A	2	0.7971	100	0.361	Contact Map
3sqnA	2	0.9239	100	0.459	Contact Map
3rioA	1	0.5978	99.9	0.599	Contact Map
3ufeA	2	0.3877	99.7	0.667	Contact Map
3nufA	2	0.4275	92.1	0.902	Contact Map
1auuA	2	0.1993	89.7	0.907	Contact Map
1l1cA	2	0.1957	89.5	0.908	Contact Map
1wwpA	2	0.2572	9.3	0.954	Contact Map
1wtyA	4	0.2609	8.4	0.955	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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