GREMLIN Database
CWLA - N-acetylmuramoyl-L-alanine amidase CwlA
UniProt: P24808 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 272 (240)
Sequences: 337 (248)
Seq/√Len: 16.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
76_H91_G7.1331.00
233_D237_G4.0761.00
153_V157_I3.3220.99
57_F114_F2.8950.98
234_G240_T2.7160.97
94_I111_A2.6840.96
55_F93_E2.5320.95
150_W154_K2.5050.95
68_I74_A2.4930.95
233_D240_T2.4720.94
134_D150_W2.4490.94
261_P265_A2.4170.94
60_D95_C2.3870.93
108_E156_A2.3300.92
112_I156_A2.2380.91
213_Q240_T2.2020.90
29_T135_W2.1920.90
116_A124_W2.1740.89
80_G85_G2.1330.88
236_Y240_T2.1310.88
57_F70_T2.1150.88
248_Q257_G2.0660.87
50_S54_S2.0660.87
37_A95_C2.0310.86
236_Y241_A2.0290.86
206_G213_Q2.0190.85
240_T243_A1.9790.84
33_T93_E1.9240.83
131_K134_D1.9230.82
111_A153_V1.9150.82
179_K183_T1.8570.80
96_Y104_Y1.8560.80
79_D136_N1.8440.80
237_G240_T1.8310.79
85_G135_W1.8310.79
116_A119_L1.7140.74
42_E46_M1.7040.74
107_A145_L1.6950.73
67_G77_T1.6930.73
248_Q256_D1.6840.73
47_K71_N1.6820.73
27_Y89_S1.6600.72
234_G237_G1.6560.72
62_K65_I1.6520.71
32_N96_Y1.6470.71
43_I46_M1.6390.71
251_Y263_T1.6130.70
58_A67_G1.6050.69
247_F253_L1.5930.69
200_K203_M1.5820.68
116_A161_L1.5700.67
250_M264_K1.5660.67
37_A42_E1.5590.67
222_Y231_G1.5250.65
246_R250_M1.5190.65
39_A43_I1.5070.64
42_E45_Y1.4950.64
223_P246_R1.4420.61
213_Q233_D1.3860.58
195_G200_K1.3850.57
244_I259_Y1.3740.57
213_Q237_G1.3730.57
38_S42_E1.3640.56
221_F243_A1.3500.55
31_H132_H1.2930.52
167_K171_S1.2870.52
54_S75_W1.2800.51
33_T37_A1.2770.51
251_Y266_K1.2730.51
241_A245_R1.2690.51
253_L262_K1.2690.51
244_I263_T1.2660.51
54_S140_C1.2530.50
236_Y243_A1.2460.49
215_A218_A1.2460.49
55_F96_Y1.2360.49
40_A44_S1.2240.48
33_T138_K1.2230.48
131_K145_L1.2180.48
210_T240_T1.2140.48
221_F250_M1.2110.47
54_S134_D1.1790.46
259_Y264_K1.1750.45
108_E112_I1.1720.45
80_G135_W1.1620.45
38_S48_N1.1620.45
245_R262_K1.1370.43
244_I253_L1.1360.43
167_K177_K1.1320.43
210_T234_G1.1300.43
159_A166_G1.1230.42
192_L195_G1.1180.42
92_V114_F1.0950.41
57_F140_C1.0950.41
69_P72_R1.0920.41
93_E96_Y1.0720.39
210_T231_G1.0610.39
117_Q121_E1.0590.39
91_G138_K1.0550.39
30_I115_V1.0480.38
54_S68_I1.0460.38
134_D154_K1.0460.38
199_V206_G1.0430.38
21_N87_R1.0420.38
94_I107_A1.0420.38
244_I247_F1.0340.37
173_A182_N1.0330.37
86_N132_H1.0310.37
247_F263_T1.0160.36
39_A42_E1.0160.36
130_R150_W1.0110.36
29_T130_R1.0110.36
237_G248_Q1.0020.36
29_T89_S0.9980.35
31_H86_N0.9880.35
195_G202_P0.9880.35
253_L263_T0.9830.35
236_Y239_K0.9820.35
217_A223_P0.9800.35
70_T121_E0.9800.35
179_K182_N0.9790.34
76_H86_N0.9750.34
79_D137_G0.9720.34
86_N91_G0.9660.34
235_V262_K0.9620.34
197_F200_K0.9480.33
54_S72_R0.9470.33
238_P242_D0.9450.33
59_V111_A0.9370.32
222_Y240_T0.9370.32
118_L129_V0.9260.32
119_L161_L0.9250.32
170_S180_T0.9240.32
163_K167_K0.9200.31
217_A232_I0.9190.31
64_V113_K0.9180.31
220_Y223_P0.9150.31
206_G209_V0.9060.31
76_H138_K0.9050.31
35_N69_P0.9020.31
213_Q222_Y0.9000.30
104_Y107_A0.8970.30
188_T191_A0.8950.30
248_Q255_Q0.8910.30
78_G93_E0.8890.30
212_I244_I0.8870.30
46_M51_S0.8860.30
32_N138_K0.8790.29
254_T262_K0.8760.29
180_T183_T0.8730.29
213_Q243_A0.8680.29
66_Q114_F0.8680.29
245_R249_S0.8670.29
212_I221_F0.8670.29
248_Q260_G0.8650.29
258_I261_P0.8650.29
55_F91_G0.8630.29
238_P245_R0.8590.28
253_L260_G0.8590.28
215_A264_K0.8590.28
259_Y263_T0.8570.28
232_I239_K0.8560.28
209_V243_A0.8520.28
54_S93_E0.8490.28
249_S259_Y0.8480.28
233_D236_Y0.8450.28
78_G86_N0.8430.28
28_I117_Q0.8410.28
166_G170_S0.8410.28
217_A241_A0.8400.28
107_A131_K0.8380.28
46_M55_F0.8360.27
35_N42_E0.8350.27
196_I202_P0.8340.27
206_G243_A0.8310.27
32_N117_Q0.8190.27
190_Y204_M0.8160.26
27_Y154_K0.8100.26
34_A42_E0.8060.26
95_C107_A0.8050.26
241_A264_K0.8050.26
124_W161_L0.7990.26
197_F204_M0.7920.25
36_D97_S0.7910.25
165_G170_S0.7850.25
177_K183_T0.7850.25
55_F58_A0.7850.25
249_S255_Q0.7830.25
27_Y104_Y0.7810.25
45_Y60_D0.7780.25
27_Y150_W0.7760.25
39_A95_C0.7700.24
230_N242_D0.7680.24
46_M58_A0.7670.24
23_M140_C0.7660.24
54_S89_S0.7630.24
13_S118_L0.7630.24
54_S66_Q0.7600.24
38_S41_N0.7570.24
38_S45_Y0.7560.24
209_V212_I0.7550.24
79_D140_C0.7550.24
249_S262_K0.7550.24
220_Y267_L0.7390.23
29_T85_G0.7380.23
163_K168_T0.7380.23
51_S70_T0.7380.23
178_K183_T0.7350.23
37_A45_Y0.7350.23
210_T233_D0.7350.23
210_T232_I0.7350.23
236_Y259_Y0.7340.23
170_S179_K0.7330.23
55_F67_G0.7320.23
74_A91_G0.7260.23
46_M54_S0.7260.23
247_F251_Y0.7260.23
240_T260_G0.7240.23
221_F264_K0.7240.23
140_C145_L0.7220.22
77_T91_G0.7210.22
89_S140_C0.7160.22
52_S78_G0.7140.22
92_V129_V0.7120.22
37_A41_N0.7120.22
258_I266_K0.7110.22
196_I200_K0.7110.22
215_A243_A0.7020.22
137_G140_C0.6940.21
32_N104_Y0.6930.21
94_I103_R0.6930.21
218_A222_Y0.6920.21
175_V178_K0.6920.21
158_E166_G0.6910.21
50_S75_W0.6900.21
242_D246_R0.6890.21
251_Y254_T0.6870.21
237_G260_G0.6860.21
26_E88_K0.6850.21
131_K150_W0.6840.21
75_W93_E0.6840.21
193_P197_F0.6830.21
190_Y198_K0.6830.21
56_H86_N0.6790.21
53_T72_R0.6760.21
55_F74_A0.6760.21
213_Q234_G0.6740.21
211_Q215_A0.6730.21
41_N45_Y0.6710.20
241_A259_Y0.6700.20
48_N118_L0.6680.20
36_D129_V0.6670.20
190_Y193_P0.6660.20
174_S177_K0.6640.20
163_K172_K0.6620.20
59_V110_L0.6590.20
231_G234_G0.6560.20
42_E95_C0.6520.20
231_G239_K0.6500.20
253_L261_P0.6480.20
138_K142_H0.6470.20
215_A220_Y0.6460.20
38_S46_M0.6450.20
129_V142_H0.6410.19
68_I75_W0.6390.19
250_M259_Y0.6380.19
64_V114_F0.6350.19
39_A63_Q0.6350.19
60_D63_Q0.6350.19
62_K110_L0.6280.19
59_V94_I0.6270.19
206_G221_F0.6230.19
235_V265_A0.6190.19
27_Y130_R0.6190.19
205_R219_L0.6170.19
55_F76_H0.6170.19
190_Y202_P0.6140.18
249_S264_K0.6120.18
74_A96_Y0.6120.18
78_G138_K0.6110.18
172_K177_K0.6110.18
210_T213_Q0.6100.18
218_A231_G0.6080.18
195_G199_V0.6070.18
233_D258_I0.6010.18
213_Q263_T0.6010.18
231_G237_G0.6010.18
221_F234_G0.6000.18
213_Q236_Y0.5950.18
231_G250_M0.5950.18
208_K215_A0.5950.18
96_Y138_K0.5940.18
163_K217_A0.5920.18
29_T140_C0.5890.18
35_N39_A0.5870.17
258_I265_A0.5860.17
46_M53_T0.5830.17
199_V215_A0.5820.17
20_P72_R0.5810.17
152_Q173_A0.5800.17
55_F68_I0.5790.17
36_D67_G0.5750.17
89_S154_K0.5750.17
121_E154_K0.5740.17
175_V179_K0.5740.17
192_L197_F0.5740.17
33_T153_V0.5690.17
53_T93_E0.5650.17
44_S47_K0.5650.17
95_C111_A0.5650.17
54_S103_R0.5640.17
44_S48_N0.5630.17
223_P234_G0.5630.17
212_I215_A0.5610.17
178_K181_T0.5600.17
106_A201_S0.5600.17
55_F107_A0.5570.17
97_S101_G0.5550.16
241_A255_Q0.5550.16
233_D243_A0.5540.16
200_K215_A0.5510.16
257_G263_T0.5500.16
32_N150_W0.5490.16
28_I115_V0.5470.16
217_A231_G0.5460.16
78_G136_N0.5440.16
205_R208_K0.5430.16
207_E220_Y0.5410.16
253_L266_K0.5410.16
188_T201_S0.5370.16
54_S57_F0.5370.16
16_G73_N0.5360.16
25_A104_Y0.5340.16
205_R235_V0.5330.16
131_K140_C0.5290.16
223_P232_I0.5290.16
192_L202_P0.5280.16
237_G257_G0.5280.16
64_V159_A0.5270.16
200_K206_G0.5270.16
96_Y140_C0.5260.16
242_D254_T0.5250.16
63_Q66_Q0.5240.15
222_Y264_K0.5190.15
176_A181_T0.5180.15
243_A247_F0.5170.15
166_G172_K0.5160.15
33_T96_Y0.5150.15
162_K165_G0.5140.15
196_I199_V0.5100.15
166_G178_K0.5090.15
173_A176_A0.5070.15
32_N91_G0.5070.15
232_I240_T0.5060.15
262_K265_A0.5010.15
91_G131_K0.5010.15
39_A46_M0.5010.15
236_Y249_S0.5000.15
89_S92_V0.5000.15
182_N201_S0.5000.15
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4bolA 2 0.8199 100 0.366 Contact Map
3d2yA 3 0.8346 100 0.373 Contact Map
4bxjA 4 0.8162 100 0.378 Contact Map
1yb0A 3 0.5846 100 0.495 Contact Map
3rdrA 4 0.5515 100 0.519 Contact Map
4ivvA 1 0.5662 100 0.521 Contact Map
4knkA 1 0.6213 100 0.523 Contact Map
4olsA 2 0.5772 100 0.526 Contact Map
1j3gA 1 0.5846 100 0.573 Contact Map
1ohtA 1 0.5772 100 0.586 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0086 seconds.