FLIY - BACSU - GREMLIN Contacts

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FLIY - Flagellar motor switch phosphatase FliY

UniProt:	P24073	Sequences: MENNRLSQDEIDALLNGTGSTLDEPEIPEVDDLSEMERDAIGEIGNISFGSSATALSTLLNQKVDITTPSVTVIPRSKISDAFPEPYVAIEVNYTEGFSGSNLLVVEQSDAAIIADLMIGGDGKGADPSLGEIHLSAVQEAMNQMMGSAATSMSTVFSKKIDISPPRVELLDVTEEKGTDRIPDDEMLVKVSFNLKVGELIDSSIMQLYPLTFAKDLISSLMNSESAEEEETVQPEVTYEQPKEPVTPEPRIEPKQQQQPPKRQGTAKKAAPVQVSPVEFSAFDPNEAVQAPIHNLDMLLDIPLSITVELGRTKRSVKEILELSAGSIIELDKLAGEPVDILVNQRIVAKGEVVVIEENFGVRVTDILSQAERINNLK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	378 (367)
Sequences:	517 (428)
Seq/√Len:	22.4

BACSU - FLIY

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
62_Q	198_G	7.301	1.00
122_D	125_G	4.411	1.00
131_G	134_H	4.016	1.00
197_V	201_I	3.990	1.00
35_E	38_R	3.725	1.00
133_I	150_A	3.620	1.00
12_D	18_T	3.611	1.00
55_A	148_S	3.311	1.00
82_A	190_K	3.175	1.00
309_E	336_G	3.175	1.00
309_E	340_D	2.819	0.99
243_K	249_E	2.792	0.99
159_K	220_S	2.719	0.99
339_V	362_V	2.695	0.99
341_I	362_V	2.650	0.99
242_P	245_P	2.627	0.99
243_K	246_V	2.607	0.99
51_S	155_T	2.558	0.98
299_L	345_Q	2.451	0.98
49_F	345_Q	2.361	0.97
245_P	248_P	2.260	0.96
92_V	221_L	2.182	0.96
248_P	251_R	2.141	0.95
115_A	138_V	2.126	0.95
111_A	138_V	2.104	0.95
251_R	257_Q	2.038	0.94
72_T	319_E	2.025	0.94
340_D	366_D	2.024	0.94
248_P	253_E	1.988	0.93
9_D	52_S	1.987	0.93
244_E	249_E	1.974	0.93
309_E	312_R	1.970	0.93
245_P	251_R	1.928	0.92
68_T	203_S	1.922	0.92
79_I	197_V	1.897	0.91
323_L	327_S	1.895	0.91
246_V	251_R	1.888	0.91
298_M	302_I	1.887	0.91
213_F	283_F	1.875	0.91
253_E	258_Q	1.855	0.90
118_M	141_A	1.855	0.90
243_K	247_T	1.835	0.90
243_K	250_P	1.833	0.89
231_E	238_T	1.832	0.89
39_D	42_G	1.813	0.89
37_E	215_K	1.793	0.88
154_S	336_G	1.782	0.88
262_K	265_G	1.778	0.88
42_G	54_T	1.778	0.88
295_N	335_A	1.748	0.87
336_G	340_D	1.721	0.86
89_A	103_L	1.716	0.86
247_T	257_Q	1.712	0.86
113_I	160_K	1.703	0.86
301_D	358_E	1.688	0.85
252_I	258_Q	1.682	0.85
252_I	255_K	1.679	0.85
60_L	201_I	1.678	0.85
245_P	250_P	1.656	0.84
195_L	299_L	1.650	0.84
40_A	221_L	1.646	0.84
280_F	356_I	1.628	0.83
104_L	148_S	1.626	0.83
306_I	343_V	1.624	0.83
251_R	255_K	1.615	0.82
335_A	339_V	1.601	0.82
247_T	251_R	1.593	0.82
244_E	250_P	1.569	0.81
44_I	217_L	1.542	0.79
9_D	158_S	1.529	0.79
189_V	214_A	1.527	0.79
162_D	170_L	1.519	0.78
106_V	299_L	1.509	0.78
39_D	54_T	1.493	0.77
88_V	135_L	1.487	0.77
333_K	356_I	1.483	0.77
78_K	81_D	1.467	0.76
283_F	329_I	1.462	0.75
42_G	46_N	1.446	0.75
309_E	366_D	1.439	0.74
111_A	115_A	1.438	0.74
213_F	336_G	1.430	0.74
336_G	366_D	1.428	0.74
134_H	366_D	1.419	0.73
12_D	133_I	1.408	0.73
246_V	256_Q	1.401	0.72
22_L	160_K	1.399	0.72
134_H	213_F	1.395	0.72
95_T	164_S	1.383	0.71
55_A	195_L	1.380	0.71
246_V	250_P	1.378	0.71
39_D	142_M	1.373	0.71
250_P	253_E	1.368	0.70
369_S	373_R	1.359	0.70
334_L	359_N	1.358	0.70
246_V	254_P	1.350	0.69
347_I	368_L	1.333	0.68
307_T	328_I	1.330	0.68
187_M	364_V	1.321	0.68
348_V	372_E	1.320	0.68
66_I	142_M	1.317	0.67
243_K	253_E	1.308	0.67
42_G	47_I	1.306	0.67
201_I	356_I	1.301	0.66
157_F	221_L	1.294	0.66
262_K	266_T	1.291	0.66
251_R	254_P	1.282	0.65
145_M	193_F	1.272	0.65
43_E	47_I	1.270	0.65
47_I	54_T	1.269	0.64
62_Q	191_V	1.266	0.64
283_F	336_G	1.255	0.64
39_D	47_I	1.241	0.63
46_N	54_T	1.238	0.62
242_P	247_T	1.234	0.62
209_Y	325_A	1.233	0.62
253_E	256_Q	1.230	0.62
309_E	362_V	1.230	0.62
256_Q	319_E	1.209	0.61
297_D	302_I	1.207	0.60
111_A	294_H	1.207	0.60
299_L	302_I	1.179	0.59
54_T	193_F	1.178	0.59
249_E	253_E	1.178	0.59
49_F	139_Q	1.174	0.58
66_I	143_N	1.168	0.58
54_T	163_I	1.167	0.58
254_P	257_Q	1.165	0.58
288_A	292_P	1.159	0.57
317_V	325_A	1.159	0.57
244_E	251_R	1.150	0.57
58_T	182_I	1.147	0.56
265_G	268_K	1.146	0.56
219_S	222_M	1.142	0.56
92_V	111_A	1.139	0.56
54_T	143_N	1.139	0.56
146_M	154_S	1.138	0.56
371_A	375_N	1.133	0.56
147_G	151_T	1.130	0.55
370_Q	373_R	1.129	0.55
75_P	299_L	1.128	0.55
168_V	302_I	1.120	0.55
129_S	150_A	1.112	0.54
44_I	211_L	1.111	0.54
242_P	251_R	1.104	0.54
111_A	323_L	1.102	0.53
49_F	52_S	1.094	0.53
230_E	250_P	1.089	0.53
63_K	191_V	1.083	0.52
106_V	111_A	1.082	0.52
246_V	257_Q	1.077	0.52
10_E	14_L	1.075	0.52
109_S	113_I	1.072	0.51
213_F	309_E	1.072	0.51
238_T	245_P	1.070	0.51
19_G	364_V	1.069	0.51
56_L	165_P	1.068	0.51
42_G	166_P	1.068	0.51
238_T	253_E	1.064	0.51
42_G	163_I	1.062	0.51
247_T	255_K	1.062	0.51
9_D	315_R	1.058	0.50
27_I	197_V	1.055	0.50
123_G	141_A	1.053	0.50
244_E	247_T	1.048	0.50
51_S	95_T	1.048	0.50
242_P	253_E	1.047	0.50
236_E	262_K	1.047	0.50
280_F	333_K	1.036	0.49
187_M	300_L	1.032	0.49
44_I	205_I	1.023	0.48
146_M	309_E	1.018	0.48
54_T	142_M	1.017	0.48
164_S	335_A	1.016	0.48
139_Q	302_I	1.016	0.48
157_F	172_D	1.014	0.47
13_A	323_L	1.011	0.47
3_N	162_D	1.008	0.47
242_P	246_V	1.004	0.47
39_D	151_T	1.000	0.46
47_I	142_M	0.997	0.46
213_F	340_D	0.987	0.46
260_P	268_K	0.987	0.46
46_N	193_F	0.985	0.45
24_E	244_E	0.981	0.45
8_Q	13_A	0.977	0.45
228_E	231_E	0.970	0.44
71_V	188_L	0.970	0.44
243_K	268_K	0.970	0.44
54_T	145_M	0.961	0.44
54_T	166_P	0.958	0.44
201_I	366_D	0.953	0.43
51_S	148_S	0.953	0.43
254_P	259_Q	0.951	0.43
232_T	260_P	0.948	0.43
77_S	243_K	0.946	0.43
154_S	322_E	0.946	0.43
296_L	347_I	0.945	0.43
334_L	339_V	0.944	0.43
51_S	83_F	0.941	0.42
42_G	50_G	0.938	0.42
102_N	193_F	0.937	0.42
51_S	106_V	0.933	0.42
69_P	143_N	0.933	0.42
256_Q	260_P	0.931	0.42
39_D	46_N	0.930	0.42
90_I	357_E	0.929	0.42
249_E	257_Q	0.928	0.42
159_K	216_D	0.928	0.42
9_D	196_K	0.927	0.42
34_S	37_E	0.926	0.41
21_T	25_P	0.925	0.41
242_P	256_Q	0.924	0.41
42_G	143_N	0.924	0.41
163_I	166_P	0.924	0.41
110_D	203_S	0.924	0.41
46_N	50_G	0.919	0.41
310_L	352_E	0.918	0.41
208_L	333_K	0.918	0.41
77_S	129_S	0.916	0.41
138_V	155_T	0.909	0.40
345_Q	364_V	0.908	0.40
69_P	193_F	0.908	0.40
12_D	57_S	0.905	0.40
91_E	135_L	0.901	0.40
244_E	254_P	0.900	0.40
351_G	359_N	0.900	0.40
244_E	255_K	0.899	0.40
300_L	314_K	0.897	0.40
49_F	296_L	0.897	0.40
186_E	229_E	0.895	0.39
49_F	299_L	0.891	0.39
350_K	366_D	0.889	0.39
247_T	250_P	0.886	0.39
300_L	345_Q	0.883	0.39
20_S	368_L	0.883	0.39
175_E	242_P	0.881	0.38
53_A	309_E	0.880	0.38
45_G	115_A	0.880	0.38
46_N	163_I	0.879	0.38
237_V	245_P	0.877	0.38
36_M	108_Q	0.873	0.38
16_N	24_E	0.868	0.38
49_F	119_I	0.867	0.38
274_Q	327_S	0.858	0.37
253_E	257_Q	0.855	0.37
42_G	193_F	0.851	0.37
338_P	356_I	0.850	0.37
245_P	255_K	0.850	0.37
251_R	262_K	0.849	0.36
50_G	54_T	0.847	0.36
248_P	258_Q	0.845	0.36
252_I	259_Q	0.845	0.36
208_L	213_F	0.843	0.36
35_E	368_L	0.842	0.36
150_A	345_Q	0.841	0.36
263_R	266_T	0.841	0.36
278_V	316_S	0.841	0.36
234_Q	237_V	0.839	0.36
215_K	313_T	0.839	0.36
243_K	251_R	0.838	0.36
339_V	353_V	0.838	0.36
23_D	30_V	0.836	0.36
119_I	131_G	0.831	0.35
243_K	257_Q	0.823	0.35
39_D	166_P	0.823	0.35
151_T	166_P	0.821	0.35
39_D	163_I	0.820	0.35
19_G	242_P	0.819	0.35
283_F	333_K	0.815	0.34
163_I	193_F	0.814	0.34
278_V	294_H	0.813	0.34
138_V	203_S	0.811	0.34
277_P	340_D	0.809	0.34
27_I	266_T	0.807	0.34
328_I	347_I	0.805	0.34
246_V	249_E	0.805	0.34
86_P	117_L	0.804	0.34
55_A	138_V	0.803	0.34
341_I	351_G	0.799	0.33
46_N	145_M	0.798	0.33
142_M	166_P	0.798	0.33
330_E	352_E	0.796	0.33
219_S	237_V	0.796	0.33
238_T	269_K	0.795	0.33
27_I	256_Q	0.792	0.33
250_P	254_P	0.792	0.33
118_M	165_P	0.791	0.33
24_E	247_T	0.789	0.33
62_Q	149_A	0.787	0.33
20_S	232_T	0.786	0.33
271_A	325_A	0.785	0.32
54_T	69_P	0.782	0.32
348_V	357_E	0.778	0.32
194_N	202_D	0.778	0.32
310_L	337_E	0.778	0.32
46_N	64_V	0.778	0.32
69_P	145_M	0.777	0.32
242_P	254_P	0.776	0.32
338_P	362_V	0.774	0.32
16_N	175_E	0.774	0.32
256_Q	259_Q	0.773	0.32
154_S	213_F	0.765	0.31
16_N	223_N	0.765	0.31
213_F	217_L	0.765	0.31
166_P	193_F	0.764	0.31
312_R	342_L	0.764	0.31
12_D	224_S	0.763	0.31
335_A	340_D	0.763	0.31
95_T	362_V	0.763	0.31
50_G	193_F	0.762	0.31
20_S	23_D	0.760	0.31
43_E	46_N	0.760	0.31
62_Q	199_E	0.758	0.31
143_N	163_I	0.756	0.31
46_N	142_M	0.755	0.31
118_M	121_G	0.755	0.31
248_P	261_P	0.754	0.31
34_S	252_I	0.750	0.30
236_E	259_Q	0.750	0.30
299_L	359_N	0.744	0.30
276_S	281_S	0.742	0.30
51_S	55_A	0.738	0.30
57_S	115_A	0.737	0.30
47_I	143_N	0.736	0.30
127_D	347_I	0.734	0.29
309_E	356_I	0.733	0.29
25_P	248_P	0.732	0.29
46_N	166_P	0.732	0.29
97_G	151_T	0.731	0.29
260_P	263_R	0.729	0.29
116_D	197_V	0.728	0.29
137_A	351_G	0.728	0.29
238_T	244_E	0.725	0.29
242_P	250_P	0.725	0.29
96_E	131_G	0.725	0.29
167_R	191_V	0.724	0.29
9_D	21_T	0.723	0.29
97_G	110_D	0.723	0.29
253_E	259_Q	0.722	0.29
248_P	254_P	0.719	0.29
340_D	356_I	0.719	0.29
9_D	61_N	0.716	0.28
79_I	89_A	0.715	0.28
39_D	193_F	0.710	0.28
24_E	237_V	0.709	0.28
122_D	126_A	0.708	0.28
203_S	295_N	0.705	0.28
164_S	339_V	0.702	0.28
349_A	354_V	0.700	0.28
79_I	103_L	0.700	0.28
8_Q	12_D	0.698	0.27
154_S	309_E	0.696	0.27
333_K	355_V	0.694	0.27
143_N	203_S	0.693	0.27
103_L	182_I	0.693	0.27
5_R	306_I	0.693	0.27
352_E	358_E	0.691	0.27
15_L	368_L	0.691	0.27
250_P	255_K	0.690	0.27
199_E	375_N	0.687	0.27
129_S	263_R	0.686	0.27
95_T	131_G	0.686	0.27
276_S	305_S	0.685	0.27
46_N	66_I	0.681	0.27
52_S	57_S	0.680	0.27
112_A	134_H	0.677	0.26
229_E	261_P	0.675	0.26
54_T	102_N	0.675	0.26
245_P	258_Q	0.675	0.26
42_G	66_I	0.669	0.26
112_A	325_A	0.667	0.26
54_T	66_I	0.664	0.26
51_S	138_V	0.663	0.26
46_N	151_T	0.663	0.26
280_F	346_R	0.663	0.26
17_G	375_N	0.661	0.26
43_E	50_G	0.661	0.26
335_A	360_F	0.661	0.26
231_E	235_P	0.655	0.25
33_L	38_R	0.655	0.25
249_E	255_K	0.655	0.25
19_G	32_D	0.653	0.25
25_P	237_V	0.652	0.25
271_A	314_K	0.651	0.25
254_P	258_Q	0.651	0.25
334_L	337_E	0.650	0.25
142_M	163_I	0.649	0.25
339_V	369_S	0.649	0.25
149_A	372_E	0.645	0.25
52_S	104_L	0.645	0.25
42_G	151_T	0.645	0.25
315_R	374_I	0.645	0.25
171_L	178_G	0.644	0.25
7_S	215_K	0.644	0.25
55_A	161_I	0.644	0.25
342_L	348_V	0.638	0.24
312_R	320_I	0.637	0.24
342_L	358_E	0.637	0.24
111_A	362_V	0.636	0.24
185_D	256_Q	0.636	0.24
83_F	359_N	0.635	0.24
102_N	166_P	0.635	0.24
86_P	145_M	0.635	0.24
36_M	235_P	0.629	0.24
176_E	182_I	0.627	0.24
102_N	143_N	0.627	0.24
332_D	363_R	0.625	0.24
172_D	287_E	0.625	0.24
115_A	139_Q	0.624	0.24
23_D	27_I	0.622	0.24
336_G	350_K	0.621	0.23
112_A	126_A	0.621	0.23
102_N	207_Q	0.621	0.23
126_A	329_I	0.619	0.23
136_S	144_Q	0.619	0.23
226_S	234_Q	0.617	0.23
66_I	141_A	0.617	0.23
185_D	258_Q	0.617	0.23
283_F	328_I	0.616	0.23
146_M	336_G	0.613	0.23
234_Q	238_T	0.612	0.23
136_S	143_N	0.612	0.23
77_S	245_P	0.611	0.23
307_T	336_G	0.610	0.23
353_V	358_E	0.610	0.23
137_A	143_N	0.610	0.23
47_I	145_M	0.607	0.23
73_V	122_D	0.606	0.23
15_L	208_L	0.604	0.23
23_D	369_S	0.600	0.22
36_M	126_A	0.598	0.22
219_S	223_N	0.597	0.22
143_N	193_F	0.595	0.22
42_G	142_M	0.595	0.22
79_I	162_D	0.594	0.22
216_D	287_E	0.594	0.22
182_I	271_A	0.592	0.22
243_K	248_P	0.591	0.22
61_N	341_I	0.590	0.22
244_E	258_Q	0.586	0.22
248_P	257_Q	0.586	0.22
65_D	146_M	0.586	0.22
22_L	246_V	0.585	0.22
200_L	205_I	0.585	0.22
230_E	258_Q	0.584	0.22
77_S	248_P	0.584	0.22
156_V	364_V	0.583	0.22
42_G	102_N	0.583	0.22
316_S	328_I	0.583	0.22
349_A	352_E	0.582	0.22
134_H	317_V	0.580	0.22
193_F	207_Q	0.580	0.22
3_N	316_S	0.577	0.21
37_E	50_G	0.577	0.21
99_S	368_L	0.577	0.21
54_T	151_T	0.575	0.21
187_M	302_I	0.575	0.21
244_E	263_R	0.574	0.21
137_A	359_N	0.574	0.21
275_V	315_R	0.573	0.21
184_D	197_V	0.572	0.21
226_S	247_T	0.571	0.21
243_K	254_P	0.570	0.21
218_I	221_L	0.570	0.21
33_L	267_A	0.570	0.21
209_Y	357_E	0.568	0.21
66_I	166_P	0.567	0.21
237_V	258_Q	0.567	0.21
295_N	333_K	0.564	0.21
49_F	83_F	0.564	0.21
17_G	278_V	0.563	0.21
46_N	49_F	0.563	0.21
337_E	352_E	0.563	0.21
122_D	201_I	0.561	0.21
245_P	256_Q	0.561	0.21
301_D	352_E	0.561	0.21
142_M	145_M	0.560	0.21
12_D	16_N	0.558	0.21
75_P	253_E	0.557	0.20
23_D	81_D	0.556	0.20
165_P	207_Q	0.556	0.20
194_N	291_A	0.556	0.20
94_Y	278_V	0.554	0.20
84_P	132_E	0.554	0.20
19_G	22_L	0.553	0.20
30_V	225_E	0.553	0.20
215_K	236_E	0.553	0.20
283_F	323_L	0.551	0.20
169_E	217_L	0.551	0.20
189_V	225_E	0.550	0.20
77_S	185_D	0.549	0.20
335_A	366_D	0.549	0.20
116_D	364_V	0.548	0.20
139_Q	204_S	0.547	0.20
126_A	314_K	0.547	0.20
224_S	315_R	0.546	0.20
280_F	283_F	0.545	0.20
227_A	233_V	0.545	0.20
69_P	166_P	0.545	0.20
232_T	244_E	0.544	0.20
296_L	299_L	0.542	0.20
47_I	163_I	0.542	0.20
39_D	102_N	0.542	0.20
295_N	309_E	0.541	0.20
34_S	233_V	0.540	0.20
91_E	131_G	0.539	0.20
244_E	253_E	0.538	0.20
50_G	143_N	0.533	0.19
78_K	265_G	0.532	0.19
245_P	252_I	0.532	0.19
128_P	285_P	0.532	0.19
43_E	54_T	0.531	0.19
19_G	219_S	0.531	0.19
247_T	254_P	0.530	0.19
108_Q	170_L	0.530	0.19
3_N	17_G	0.529	0.19
341_I	357_E	0.529	0.19
213_F	366_D	0.528	0.19
226_S	242_P	0.526	0.19
136_S	163_I	0.522	0.19
17_G	162_D	0.521	0.19
338_P	352_E	0.516	0.19
253_E	291_A	0.515	0.19
114_I	203_S	0.515	0.19
226_S	254_P	0.514	0.19
162_D	298_M	0.513	0.19
320_I	333_K	0.512	0.19
58_T	169_E	0.512	0.19
338_P	353_V	0.511	0.18
16_N	362_V	0.510	0.18
9_D	341_I	0.510	0.18
46_N	143_N	0.510	0.18
248_P	260_P	0.509	0.18
47_I	193_F	0.509	0.18
218_I	319_E	0.509	0.18
356_I	366_D	0.507	0.18
19_G	73_V	0.506	0.18
84_P	332_D	0.506	0.18
17_G	345_Q	0.506	0.18
89_A	105_V	0.506	0.18
118_M	207_Q	0.505	0.18
217_L	283_F	0.504	0.18
139_Q	324_S	0.504	0.18
47_I	102_N	0.503	0.18
16_N	297_D	0.503	0.18
249_E	261_P	0.502	0.18
84_P	221_L	0.500	0.18
352_E	361_G	0.500	0.18
143_N	147_G	0.500	0.18

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4hynA	1	0.5265	100	0.744	Contact Map
3qtaA	2	0.5291	100	0.765	Contact Map
1xkrA	1	0.5106	100	0.773	Contact Map
4fhrA	1	0.4788	100	0.776	Contact Map
4gc8A	1	0.4815	100	0.778	Contact Map
4yxcB	1	0.4365	100	0.783	Contact Map
1o6aA	2	0.2275	99.9	0.818	Contact Map
1squA	2	0.4048	99.9	0.823	Contact Map
3hm4A	2	0.4048	99.9	0.83	Contact Map
3h2dA	2	0.4048	99.9	0.83	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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