FLIM - BACSU - GREMLIN Contacts

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FLIM - Flagellar motor switch protein FliM

UniProt:	P23453	Sequences: MSGEVLSQNEIDALLSAISTGEMDAEELKKEEKEKKVKVYDFKRALRFSKDQIRSLTRIHDNFARLLTTHFSAQLRTYIHISVSSVDQVPYEEFIRSIPNMTILNLFDVHPMEGRIMMEVNPTIAYTMMDRVMGGIGISHNKVDSLTEIETKIISNLFENALGNYKEAWQSIADIEPEMTEFEVNPQFVQMVSPNETVVVISLNTQIGEISGVINLCIPHIVLEPLIPKLSVHYWMQSDRNEPKPEETKSLEKRIMTAQIPVVAELGTSELTIEEFLSLEVGDCITLDKSVTDPLTVLVGDKPKFLGQAGRVNRKQAVQILDHDIRGEQDGE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	332 (311)
Sequences:	1937 (1306)
Seq/√Len:	74.0

ECOLI - FLIM
BACSU - FLIM FLIY

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
264_A	295_L	5.895	1.00
297_V	307_G	5.421	1.00
130_D	137_G	5.398	1.00
265_E	268_T	4.937	1.00
306_L	322_D	4.929	1.00
147_T	150_E	4.198	1.00
67_L	161_A	3.832	1.00
8_Q	12_D	3.802	1.00
77_T	208_G	3.595	1.00
297_V	318_V	3.576	1.00
271_L	276_F	3.278	1.00
296_T	306_L	3.207	1.00
307_G	318_V	3.156	1.00
294_P	321_L	3.127	1.00
125_A	154_I	3.097	1.00
133_M	153_I	2.967	1.00
264_A	297_V	2.750	1.00
296_T	303_P	2.688	1.00
70_H	164_N	2.684	1.00
21_G	25_A	2.623	1.00
279_L	283_D	2.622	1.00
271_L	279_L	2.526	1.00
12_D	18_I	2.455	1.00
267_G	287_L	2.402	1.00
50_K	53_I	2.316	1.00
265_E	296_T	2.043	1.00
265_E	298_L	1.964	1.00
93_E	96_R	1.945	1.00
273_I	276_F	1.928	1.00
220_H	224_E	1.914	1.00
155_S	159_E	1.913	1.00
130_D	135_G	1.849	1.00
21_G	24_D	1.810	1.00
128_M	157_L	1.775	1.00
202_S	213_V	1.775	1.00
23_M	28_L	1.755	1.00
106_L	115_R	1.752	1.00
110_H	178_E	1.735	1.00
97_S	200_V	1.725	1.00
263_V	301_D	1.717	1.00
274_E	278_S	1.691	1.00
166_K	176_E	1.677	1.00
308_Q	321_L	1.673	1.00
23_M	26_E	1.665	1.00
289_K	296_T	1.662	1.00
198_V	217_C	1.647	1.00
261_P	300_G	1.636	1.00
23_M	27_E	1.630	1.00
133_M	149_I	1.606	0.99
107_F	162_L	1.603	0.99
294_P	308_Q	1.578	0.99
58_R	181_E	1.570	0.99
24_D	28_L	1.533	0.99
303_P	324_D	1.533	0.99
104_L	117_M	1.494	0.99
262_V	299_V	1.488	0.99
41_D	45_A	1.443	0.99
244_K	247_E	1.439	0.99
8_Q	11_I	1.437	0.99
207_I	211_S	1.430	0.99
105_N	158_F	1.424	0.99
263_V	298_L	1.419	0.99
172_I	230_L	1.409	0.99
16_S	19_S	1.400	0.99
48_F	53_I	1.395	0.98
91_Y	198_V	1.382	0.98
136_I	139_S	1.377	0.98
262_V	305_F	1.375	0.98
109_V	162_L	1.369	0.98
92_E	96_R	1.369	0.98
116_I	216_L	1.350	0.98
295_L	318_V	1.343	0.98
10_E	14_L	1.341	0.98
110_H	176_E	1.336	0.98
272_T	275_E	1.333	0.98
17_A	21_G	1.331	0.98
19_S	22_E	1.315	0.98
103_I	146_L	1.314	0.98
20_T	23_M	1.312	0.98
311_R	314_R	1.306	0.98
93_E	97_S	1.303	0.98
24_D	27_E	1.299	0.98
94_F	217_C	1.291	0.97
98_I	102_T	1.289	0.97
312_V	317_A	1.285	0.97
12_D	99_P	1.277	0.97
127_T	131_R	1.253	0.97
116_I	218_I	1.246	0.97
18_I	21_G	1.246	0.97
83_V	203_L	1.244	0.97
55_S	230_L	1.244	0.97
307_G	311_R	1.243	0.97
272_T	326_R	1.242	0.97
118_M	216_L	1.240	0.97
65_R	76_R	1.239	0.97
90_P	93_E	1.236	0.97
118_M	178_E	1.214	0.96
273_I	285_I	1.213	0.96
8_Q	15_L	1.208	0.96
179_M	182_F	1.202	0.96
7_S	15_L	1.202	0.96
233_H	320_I	1.179	0.96
105_N	179_M	1.168	0.95
9_N	21_G	1.164	0.95
25_A	28_L	1.163	0.95
243_P	248_T	1.161	0.95
108_D	115_R	1.153	0.95
124_I	205_T	1.152	0.95
107_F	116_I	1.151	0.95
6_L	10_E	1.151	0.95
86_V	179_M	1.146	0.95
264_A	280_E	1.144	0.95
121_N	212_G	1.136	0.95
5_V	18_I	1.129	0.95
22_E	27_E	1.127	0.94
170_Q	292_T	1.124	0.94
71_F	223_L	1.119	0.94
40_Y	45_A	1.117	0.94
167_E	170_Q	1.115	0.94
166_K	177_P	1.111	0.94
20_T	27_E	1.110	0.94
20_T	25_A	1.109	0.94
168_A	230_L	1.105	0.94
106_L	183_E	1.103	0.94
269_S	287_L	1.099	0.94
267_G	311_R	1.095	0.94
310_G	317_A	1.093	0.94
189_V	192_V	1.086	0.93
126_Y	150_E	1.084	0.93
92_E	185_N	1.084	0.93
147_T	162_L	1.081	0.93
85_S	213_V	1.077	0.93
109_V	116_I	1.070	0.93
294_P	306_L	1.068	0.93
175_I	229_K	1.067	0.93
80_H	206_Q	1.056	0.92
218_I	326_R	1.054	0.92
273_I	277_L	1.043	0.92
124_I	212_G	1.043	0.92
264_A	305_F	1.035	0.92
308_Q	319_Q	1.034	0.92
69_T	188_F	1.033	0.91
48_F	90_P	1.028	0.91
48_F	51_D	1.027	0.91
103_I	132_V	1.024	0.91
108_D	180_T	1.002	0.90
255_I	302_K	0.996	0.90
9_N	17_A	0.996	0.90
61_D	65_R	0.995	0.90
139_S	142_K	0.983	0.89
197_T	220_H	0.977	0.89
21_G	27_E	0.973	0.89
94_F	215_N	0.971	0.89
277_L	287_L	0.965	0.88
168_A	301_D	0.963	0.88
19_S	25_A	0.961	0.88
255_I	258_A	0.959	0.88
22_E	25_A	0.955	0.88
51_D	90_P	0.952	0.88
72_S	304_K	0.949	0.88
4_E	17_A	0.942	0.87
290_S	293_D	0.941	0.87
157_L	293_D	0.936	0.87
20_T	24_D	0.927	0.86
106_L	150_E	0.923	0.86
99_P	195_N	0.916	0.86
16_S	20_T	0.914	0.86
169_W	196_E	0.913	0.85
42_F	45_A	0.911	0.85
56_L	199_V	0.910	0.85
19_S	23_M	0.909	0.85
63_F	165_Y	0.905	0.85
222_V	226_L	0.903	0.85
132_V	252_E	0.902	0.85
18_I	23_M	0.900	0.85
60_H	169_W	0.896	0.84
187_Q	190_Q	0.896	0.84
267_G	299_V	0.895	0.84
298_L	307_G	0.891	0.84
71_F	157_L	0.890	0.84
60_H	201_I	0.888	0.84
56_L	138_I	0.886	0.84
66_L	318_V	0.885	0.84
21_G	324_D	0.876	0.83
52_Q	224_E	0.874	0.83
56_L	104_L	0.873	0.83
81_I	205_T	0.873	0.83
56_L	136_I	0.868	0.82
9_N	13_A	0.864	0.82
84_S	204_N	0.862	0.82
175_I	225_P	0.862	0.82
286_T	291_V	0.862	0.82
21_G	42_F	0.859	0.82
48_F	92_E	0.857	0.82
239_D	248_T	0.856	0.82
165_Y	179_M	0.856	0.82
42_F	47_R	0.853	0.81
83_V	212_G	0.853	0.81
111_P	114_G	0.847	0.81
45_A	50_K	0.846	0.81
41_D	44_R	0.842	0.81
252_E	255_I	0.836	0.80
170_Q	207_I	0.834	0.80
132_V	307_G	0.834	0.80
94_F	104_L	0.829	0.79
116_I	158_F	0.829	0.79
16_S	23_M	0.821	0.79
312_V	319_Q	0.817	0.78
149_I	219_P	0.816	0.78
13_A	56_L	0.815	0.78
51_D	115_R	0.811	0.78
113_E	242_E	0.810	0.78
24_D	285_I	0.810	0.78
147_T	198_V	0.809	0.78
91_Y	95_I	0.809	0.78
218_I	225_P	0.808	0.78
183_E	191_M	0.805	0.77
148_E	247_E	0.804	0.77
116_I	162_L	0.803	0.77
295_L	307_G	0.801	0.77
70_H	160_N	0.800	0.77
257_T	302_K	0.799	0.77
150_E	292_T	0.798	0.77
312_V	315_K	0.797	0.77
252_E	256_M	0.796	0.77
267_G	289_K	0.796	0.77
149_I	230_L	0.796	0.77
258_A	305_F	0.790	0.76
86_V	199_V	0.786	0.76
151_T	155_S	0.778	0.75
170_Q	306_L	0.778	0.75
17_A	301_D	0.770	0.74
126_Y	144_D	0.770	0.74
112_M	225_P	0.767	0.74
227_I	230_L	0.765	0.74
130_D	320_I	0.760	0.73
16_S	24_D	0.760	0.73
7_S	10_E	0.755	0.73
54_R	57_T	0.753	0.73
120_V	214_I	0.750	0.72
205_T	214_I	0.749	0.72
79_I	201_I	0.749	0.72
46_L	88_Q	0.747	0.72
74_Q	160_N	0.747	0.72
193_S	196_E	0.747	0.72
64_A	301_D	0.744	0.72
139_S	216_L	0.743	0.72
263_V	321_L	0.742	0.72
150_E	322_D	0.728	0.70
120_V	197_T	0.725	0.70
228_P	244_K	0.724	0.70
95_I	186_P	0.722	0.69
38_K	83_V	0.721	0.69
188_F	194_P	0.721	0.69
58_R	264_A	0.719	0.69
268_T	298_L	0.719	0.69
179_M	184_V	0.714	0.69
11_I	18_I	0.713	0.68
286_T	289_K	0.709	0.68
155_S	259_Q	0.707	0.68
117_M	185_N	0.706	0.68
267_G	288_D	0.701	0.67
240_R	247_E	0.701	0.67
230_L	288_D	0.699	0.67
144_D	150_E	0.698	0.67
7_S	14_L	0.696	0.67
295_L	309_A	0.696	0.67
48_F	52_Q	0.693	0.66
185_N	189_V	0.693	0.66
213_V	255_I	0.691	0.66
219_P	279_L	0.691	0.66
19_S	108_D	0.689	0.66
218_I	265_E	0.686	0.65
292_T	296_T	0.683	0.65
51_D	237_Q	0.682	0.65
224_E	230_L	0.681	0.65
87_D	243_P	0.680	0.65
5_V	21_G	0.678	0.65
135_G	219_P	0.676	0.64
101_M	220_H	0.675	0.64
88_Q	195_N	0.673	0.64
5_V	9_N	0.673	0.64
120_V	125_A	0.672	0.64
17_A	23_M	0.672	0.64
41_D	190_Q	0.672	0.64
289_K	293_D	0.670	0.64
291_V	295_L	0.665	0.63
103_I	151_T	0.664	0.63
273_I	281_V	0.664	0.63
133_M	294_P	0.656	0.62
304_K	309_A	0.655	0.62
106_L	147_T	0.655	0.62
43_K	275_E	0.652	0.62
261_P	325_I	0.649	0.61
242_E	246_E	0.648	0.61
81_I	203_L	0.648	0.61
19_S	26_E	0.647	0.61
164_N	168_A	0.647	0.61
250_S	254_R	0.646	0.61
265_E	292_T	0.644	0.61
49_S	52_Q	0.642	0.60
45_A	239_D	0.642	0.60
54_R	62_N	0.641	0.60
26_E	326_R	0.641	0.60
260_I	277_L	0.640	0.60
251_L	310_G	0.640	0.60
42_F	49_S	0.639	0.60
163_G	167_E	0.638	0.60
199_V	275_E	0.636	0.60
100_N	143_V	0.636	0.60
104_L	107_F	0.635	0.60
18_I	26_E	0.635	0.60
305_F	323_H	0.635	0.60
124_I	158_F	0.635	0.60
102_T	105_N	0.633	0.59
163_G	307_G	0.632	0.59
183_E	225_P	0.630	0.59
92_E	186_P	0.629	0.59
72_S	79_I	0.628	0.59
283_D	310_G	0.626	0.58
298_L	311_R	0.626	0.58
43_K	50_K	0.624	0.58
311_R	323_H	0.620	0.58
46_L	51_D	0.619	0.58
40_Y	43_K	0.618	0.57
238_S	242_E	0.616	0.57
248_T	252_E	0.616	0.57
89_V	192_V	0.615	0.57
263_V	309_A	0.614	0.57
118_M	249_K	0.612	0.57
251_L	260_I	0.611	0.57
271_L	275_E	0.611	0.57
170_Q	288_D	0.610	0.56
114_G	224_E	0.609	0.56
298_L	303_P	0.607	0.56
237_Q	240_R	0.605	0.56
265_E	318_V	0.604	0.56
272_T	278_S	0.604	0.56
101_M	143_V	0.604	0.56
52_Q	57_T	0.602	0.56
97_S	275_E	0.601	0.55
109_V	175_I	0.600	0.55
88_Q	196_E	0.599	0.55
114_G	128_M	0.598	0.55
196_E	283_D	0.597	0.55
92_E	194_P	0.596	0.55
214_I	266_L	0.594	0.55
9_N	67_L	0.593	0.54
144_D	191_M	0.593	0.54
203_L	260_I	0.593	0.54
274_E	316_Q	0.592	0.54
47_R	197_T	0.591	0.54
187_Q	197_T	0.591	0.54
119_E	304_K	0.590	0.54
150_E	222_V	0.589	0.54
22_E	28_L	0.585	0.53
125_A	290_S	0.584	0.53
166_K	301_D	0.584	0.53
309_A	316_Q	0.581	0.53
112_M	226_L	0.581	0.53
113_E	133_M	0.580	0.53
294_P	322_D	0.578	0.53
82_S	152_K	0.577	0.52
147_T	219_P	0.577	0.52
70_H	153_I	0.577	0.52
290_S	314_R	0.576	0.52
41_D	91_Y	0.575	0.52
261_P	301_D	0.575	0.52
188_F	225_P	0.574	0.52
184_V	237_Q	0.571	0.52
5_V	17_A	0.570	0.52
26_E	275_E	0.569	0.51
185_N	225_P	0.569	0.51
180_T	297_V	0.569	0.51
54_R	227_I	0.568	0.51
232_V	237_Q	0.565	0.51
108_D	214_I	0.565	0.51
4_E	22_E	0.564	0.51
263_V	269_S	0.563	0.51
275_E	314_R	0.563	0.51
268_T	296_T	0.563	0.51
82_S	211_S	0.562	0.51
264_A	307_G	0.562	0.51
18_I	92_E	0.562	0.51
155_S	213_V	0.561	0.50
202_S	215_N	0.561	0.50
154_I	255_I	0.561	0.50
68_T	83_V	0.560	0.50
68_T	71_F	0.559	0.50
159_E	170_Q	0.559	0.50
253_K	306_L	0.559	0.50
41_D	47_R	0.558	0.50
174_D	279_L	0.557	0.50
131_R	167_E	0.557	0.50
106_L	191_M	0.557	0.50
101_M	128_M	0.555	0.50
79_I	152_K	0.555	0.50
123_T	204_N	0.554	0.50
257_T	260_I	0.553	0.49
156_N	181_E	0.552	0.49
108_D	288_D	0.551	0.49
118_M	278_S	0.551	0.49
266_L	290_S	0.550	0.49
51_D	54_R	0.550	0.49
40_Y	44_R	0.549	0.49
111_P	176_E	0.549	0.49
91_Y	115_R	0.549	0.49
110_H	121_N	0.548	0.49
270_E	323_H	0.547	0.49
177_P	297_V	0.546	0.49
107_F	177_P	0.546	0.49
125_A	273_I	0.544	0.48
293_D	325_I	0.544	0.48
94_F	187_Q	0.542	0.48
94_F	117_M	0.542	0.48
46_L	220_H	0.541	0.48
296_T	321_L	0.541	0.48
226_L	249_K	0.541	0.48
99_P	230_L	0.539	0.48
75_L	137_G	0.539	0.48
132_V	158_F	0.539	0.48
56_L	59_I	0.537	0.47
163_G	179_M	0.536	0.47
188_F	193_S	0.535	0.47
141_N	292_T	0.532	0.47
242_E	250_S	0.531	0.47
172_I	320_I	0.531	0.47
275_E	319_Q	0.528	0.46
265_E	312_V	0.528	0.46
205_T	315_K	0.527	0.46
264_A	289_K	0.526	0.46
204_N	211_S	0.525	0.46
182_F	216_L	0.525	0.46
97_S	186_P	0.525	0.46
267_G	293_D	0.524	0.46
51_D	142_K	0.523	0.46
92_E	196_E	0.523	0.46
176_E	260_I	0.522	0.46
287_L	294_P	0.521	0.45
93_E	183_E	0.520	0.45
255_I	262_V	0.519	0.45
111_P	126_Y	0.516	0.45
4_E	7_S	0.514	0.45
258_A	312_V	0.514	0.45
117_M	281_V	0.513	0.44
47_R	50_K	0.513	0.44
52_Q	62_N	0.513	0.44
265_E	271_L	0.513	0.44
260_I	299_V	0.511	0.44
285_I	292_T	0.510	0.44
84_S	300_G	0.509	0.44
22_E	26_E	0.508	0.44
103_I	182_F	0.508	0.44
301_D	320_I	0.508	0.44
126_Y	283_D	0.507	0.44
93_E	147_T	0.507	0.44
4_E	21_G	0.506	0.44
255_I	281_V	0.506	0.44
277_L	298_L	0.506	0.44
277_L	323_H	0.504	0.43
262_V	278_S	0.501	0.43
46_L	197_T	0.501	0.43
260_I	315_K	0.501	0.43
247_E	310_G	0.500	0.43
147_T	185_N	0.500	0.43
69_T	127_T	0.500	0.43

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4gc8A	1	0.5542	100	0.506	Contact Map
4fhrA	1	0.5482	100	0.594	Contact Map
4hynA	1	0.5753	100	0.642	Contact Map
1xkrA	1	0.5602	100	0.642	Contact Map
4yxcB	1	0.4789	99.9	0.691	Contact Map
3qtaA	2	0.5783	99.9	0.712	Contact Map
1o6aA	2	0.253	99.8	0.737	Contact Map
3uepA	2	0.247	99.8	0.747	Contact Map
4yxbD	2	0.2319	99.8	0.753	Contact Map
4tt9A	2	0.2199	99.8	0.755	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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