FLGG - BACSU - GREMLIN Contacts

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FLGG - Flagellar basal-body rod protein FlgG

UniProt:	P23446	Sequences: MLRSLYSGISGMKNFQTKLDVIGNNIANVNTVGFKKSRVTFKDMVSQTIAGGSAAGATIGGTNSKQIGLGSSSGTIDTIHSTSATQSTGRTLDLAIDGDGYFRIDTGDGTAYTRAGNFYLDNTGTLVTGDGYHVLNMNGGTIKIPTDAQSFSIGSDGKVSIVDAEGKTQDGGQIGIVTFANSDGLDKIGSNLYRESLNSGTASAANQPGDGGTGALKSGFLEMSNVDLTDEFTEMIVAQRGFQSNSKIITTSDEILQELVNLKR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	264 (245)
Sequences:	5700 (4224)
Seq/√Len:	269.9

ECOLI - FLGF FLGE FLGG
BACSU - FLGG FLHP FLHO

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
102_F	118_F	3.703	1.00
94_L	153_I	3.410	1.00
154_G	158_K	3.319	1.00
188_I	194_R	3.175	1.00
121_D	125_T	3.120	1.00
112_Y	179_F	3.049	1.00
85_T	220_F	3.002	1.00
128_T	132_Y	2.999	1.00
125_T	133_H	2.941	1.00
103_R	177_V	2.818	1.00
85_T	95_A	2.777	1.00
161_I	171_G	2.749	1.00
99_D	178_T	2.543	1.00
186_D	194_R	2.475	1.00
217_K	220_F	2.422	1.00
158_K	170_D	2.360	1.00
80_H	101_Y	2.294	1.00
106_T	111_A	2.267	1.00
254_E	257_Q	2.238	1.00
186_D	196_S	2.157	1.00
104_I	113_T	2.108	1.00
195_E	201_T	2.064	1.00
106_T	110_T	2.052	1.00
86_Q	223_M	2.028	1.00
36_K	231_E	2.015	1.00
37_S	187_K	2.013	1.00
94_L	216_L	2.012	1.00
111_A	188_I	2.011	1.00
19_L	235_M	1.941	1.00
87_S	220_F	1.931	1.00
18_K	238_A	1.931	1.00
4_S	252_S	1.922	1.00
111_A	192_L	1.851	1.00
26_I	228_L	1.828	1.00
96_I	214_G	1.823	1.00
105_D	135_L	1.821	1.00
115_A	191_N	1.815	1.00
126_L	134_V	1.805	1.00
247_K	250_T	1.802	1.00
154_G	160_S	1.786	1.00
97_D	217_K	1.762	1.00
22_I	238_A	1.758	1.00
104_I	128_T	1.743	1.00
160_S	170_D	1.737	1.00
96_I	176_I	1.728	1.00
18_K	234_E	1.719	1.00
181_N	184_G	1.702	1.00
106_T	109_G	1.694	1.00
19_L	238_A	1.679	1.00
230_D	234_E	1.644	1.00
121_D	127_V	1.638	1.00
14_N	71_S	1.626	1.00
148_A	161_I	1.626	1.00
153_I	159_V	1.602	1.00
227_D	230_D	1.587	1.00
92_L	151_F	1.587	1.00
118_F	134_V	1.537	1.00
88_T	93_D	1.528	1.00
32_V	221_L	1.511	1.00
99_D	182_S	1.496	1.00
179_F	195_E	1.492	1.00
125_T	143_K	1.467	1.00
155_S	215_A	1.453	1.00
32_V	115_A	1.440	1.00
111_A	194_R	1.435	1.00
234_E	237_V	1.425	1.00
107_G	132_Y	1.397	1.00
41_F	45_V	1.396	1.00
22_I	235_M	1.394	1.00
136_N	175_G	1.369	1.00
21_V	39_V	1.369	1.00
8_G	248_I	1.368	1.00
15_F	241_G	1.365	1.00
16_Q	20_D	1.360	1.00
78_T	187_K	1.357	1.00
115_A	129_G	1.350	1.00
106_T	132_Y	1.347	1.00
94_L	102_F	1.346	1.00
84_A	223_M	1.341	1.00
231_E	234_E	1.317	1.00
158_K	173_Q	1.304	1.00
250_T	253_D	1.300	1.00
94_L	174_I	1.291	1.00
29_V	228_L	1.285	1.00
103_R	135_L	1.283	1.00
36_K	230_D	1.270	1.00
36_K	79_I	1.266	1.00
92_L	126_L	1.264	1.00
137_M	173_Q	1.237	1.00
17_T	73_S	1.223	1.00
178_T	213_T	1.221	1.00
188_I	192_L	1.210	1.00
90_R	93_D	1.200	1.00
32_V	117_N	1.193	1.00
37_S	78_T	1.189	1.00
209_G	215_A	1.187	1.00
101_Y	182_S	1.179	1.00
132_Y	192_L	1.177	1.00
104_I	118_F	1.173	1.00
139_G	142_I	1.163	1.00
24_N	34_F	1.160	1.00
195_E	199_S	1.154	1.00
39_V	73_S	1.154	1.00
149_Q	164_A	1.148	1.00
144_I	148_A	1.140	1.00
80_H	193_Y	1.139	1.00
104_I	192_L	1.121	1.00
104_I	111_A	1.111	1.00
38_R	77_D	1.100	1.00
94_L	218_S	1.098	1.00
34_F	37_S	1.091	1.00
128_T	134_V	1.091	1.00
146_T	164_A	1.090	1.00
184_G	196_S	1.072	1.00
257_Q	261_N	1.038	1.00
199_S	202_A	1.033	1.00
253_D	256_L	1.026	1.00
136_N	172_G	1.015	1.00
11_G	245_N	1.014	1.00
130_D	190_S	1.006	1.00
92_L	120_L	0.994	1.00
38_R	79_I	0.994	1.00
15_F	238_A	0.988	1.00
136_N	142_I	0.984	1.00
103_R	175_G	0.981	1.00
22_I	231_E	0.979	1.00
97_D	215_A	0.975	1.00
240_R	243_Q	0.969	1.00
15_F	18_K	0.962	1.00
150_S	162_V	0.957	1.00
176_I	214_G	0.954	1.00
179_F	185_L	0.952	1.00
66_Q	187_K	0.951	1.00
196_S	199_S	0.951	1.00
163_D	166_G	0.950	1.00
112_Y	202_A	0.949	1.00
19_L	255_I	0.937	1.00
155_S	217_K	0.935	1.00
160_S	168_T	0.913	1.00
94_L	118_F	0.912	1.00
162_V	168_T	0.911	1.00
21_V	34_F	0.902	1.00
162_V	166_G	0.902	1.00
25_N	231_E	0.897	1.00
256_L	260_V	0.891	1.00
91_T	150_S	0.890	1.00
18_K	22_I	0.888	1.00
187_K	193_Y	0.883	1.00
135_L	141_T	0.879	1.00
137_M	175_G	0.877	1.00
96_I	102_F	0.875	1.00
95_A	220_F	0.868	1.00
176_I	213_T	0.865	1.00
145_P	161_I	0.860	1.00
2_L	256_L	0.854	1.00
90_R	119_Y	0.846	1.00
9_I	12_M	0.843	1.00
134_V	174_I	0.833	0.99
105_D	133_H	0.832	0.99
179_F	202_A	0.825	0.99
145_P	148_A	0.822	0.99
36_K	225_N	0.819	0.99
208_P	216_L	0.819	0.99
229_T	232_F	0.814	0.99
19_L	239_Q	0.813	0.99
146_T	165_E	0.809	0.99
119_Y	129_G	0.809	0.99
93_D	117_N	0.804	0.99
48_T	51_G	0.801	0.99
232_F	250_T	0.786	0.99
143_K	167_K	0.784	0.99
96_I	100_G	0.779	0.99
102_F	174_I	0.778	0.99
117_N	129_G	0.776	0.99
41_F	44_M	0.772	0.99
163_D	169_Q	0.770	0.99
240_R	253_D	0.769	0.99
133_H	141_T	0.767	0.99
23_G	248_I	0.767	0.99
11_G	15_F	0.764	0.99
250_T	254_E	0.763	0.99
126_L	142_I	0.763	0.99
85_T	217_K	0.761	0.99
44_M	48_T	0.760	0.99
113_T	128_T	0.749	0.99
165_E	168_T	0.747	0.99
32_V	223_M	0.745	0.99
18_K	237_V	0.744	0.99
209_G	212_G	0.741	0.99
43_D	46_S	0.740	0.99
3_R	69_L	0.737	0.99
139_G	175_G	0.735	0.99
12_M	246_S	0.733	0.99
251_T	254_E	0.726	0.99
127_V	133_H	0.714	0.98
5_L	249_I	0.711	0.98
42_K	72_S	0.708	0.98
143_K	146_T	0.707	0.98
252_S	255_I	0.706	0.98
8_G	249_I	0.705	0.98
82_T	97_D	0.704	0.98
13_K	227_D	0.702	0.98
126_L	174_I	0.702	0.98
226_V	231_E	0.701	0.98
128_T	192_L	0.701	0.98
100_G	214_G	0.699	0.98
40_T	77_D	0.698	0.98
142_I	146_T	0.698	0.98
249_I	253_D	0.694	0.98
258_E	261_N	0.693	0.98
70_G	73_S	0.693	0.98
172_G	175_G	0.691	0.98
178_T	182_S	0.690	0.98
228_L	243_Q	0.688	0.98
8_G	245_N	0.687	0.98
242_F	246_S	0.686	0.98
250_T	257_Q	0.685	0.98
105_D	108_D	0.680	0.98
148_A	164_A	0.678	0.98
149_Q	162_V	0.675	0.98
16_Q	242_F	0.675	0.98
10_S	14_N	0.673	0.98
4_S	248_I	0.672	0.98
46_S	68_G	0.667	0.98
172_G	177_V	0.666	0.98
159_V	172_G	0.664	0.97
137_M	142_I	0.664	0.97
38_R	41_F	0.663	0.97
40_T	44_M	0.661	0.97
2_L	255_I	0.660	0.97
209_G	214_G	0.659	0.97
176_I	179_F	0.658	0.97
188_I	191_N	0.652	0.97
52_G	64_S	0.651	0.97
231_E	239_Q	0.649	0.97
138_N	142_I	0.648	0.97
4_S	7_S	0.646	0.97
156_D	173_Q	0.644	0.97
94_L	134_V	0.643	0.97
163_D	167_K	0.643	0.97
181_N	185_L	0.639	0.97
243_Q	247_K	0.638	0.97
174_I	216_L	0.638	0.97
146_T	170_D	0.636	0.97
120_L	126_L	0.636	0.97
86_Q	221_L	0.636	0.97
121_D	133_H	0.632	0.97
229_T	237_V	0.630	0.97
40_T	75_T	0.630	0.97
146_T	161_I	0.630	0.97
141_T	144_I	0.630	0.97
142_I	145_P	0.625	0.96
255_I	259_L	0.623	0.96
211_G	214_G	0.623	0.96
104_I	134_V	0.618	0.96
68_G	76_I	0.618	0.96
147_D	163_D	0.614	0.96
125_T	141_T	0.613	0.96
184_G	200_G	0.613	0.96
91_T	218_S	0.612	0.96
42_K	74_G	0.612	0.96
246_S	255_I	0.611	0.96
113_T	116_G	0.610	0.96
142_I	172_G	0.609	0.96
7_S	70_G	0.607	0.96
248_I	252_S	0.606	0.96
49_I	52_G	0.603	0.96
13_K	230_D	0.602	0.96
96_I	216_L	0.599	0.95
5_L	256_L	0.599	0.95
117_N	221_L	0.598	0.95
14_N	41_F	0.597	0.95
104_I	132_Y	0.597	0.95
138_N	172_G	0.597	0.95
100_G	114_R	0.595	0.95
152_S	162_V	0.594	0.95
12_M	15_F	0.593	0.95
172_G	176_I	0.592	0.95
6_Y	237_V	0.590	0.95
209_G	213_T	0.589	0.95
154_G	170_D	0.589	0.95
34_F	38_R	0.589	0.95
251_T	255_I	0.588	0.95
151_F	161_I	0.587	0.95
82_T	85_T	0.586	0.95
100_G	176_I	0.584	0.95
247_K	258_E	0.582	0.95
107_G	110_T	0.582	0.95
179_F	200_G	0.581	0.95
47_Q	69_L	0.581	0.95
232_F	258_E	0.580	0.95
136_N	139_G	0.578	0.94
17_T	21_V	0.578	0.94
101_Y	193_Y	0.577	0.94
170_D	173_Q	0.573	0.94
102_F	116_G	0.571	0.94
139_G	172_G	0.571	0.94
184_G	199_S	0.570	0.94
173_Q	176_I	0.570	0.94
155_S	159_V	0.569	0.94
90_R	221_L	0.566	0.94
21_V	73_S	0.565	0.94
178_T	203_S	0.560	0.93
196_S	200_G	0.560	0.93
105_D	109_G	0.559	0.93
21_V	76_I	0.558	0.93
184_G	198_N	0.556	0.93
48_T	66_Q	0.556	0.93
49_I	53_S	0.556	0.93
3_R	20_D	0.555	0.93
13_K	17_T	0.554	0.93
94_L	219_G	0.554	0.93
239_Q	246_S	0.554	0.93
96_I	101_Y	0.553	0.93
108_D	111_A	0.553	0.93
103_R	112_Y	0.552	0.93
119_Y	122_N	0.550	0.93
15_F	233_T	0.549	0.93
177_V	180_A	0.548	0.93
248_I	251_T	0.548	0.93
144_I	171_G	0.548	0.93
103_R	174_I	0.548	0.93
7_S	12_M	0.547	0.93
48_T	52_G	0.546	0.93
244_S	248_I	0.545	0.93
5_L	11_G	0.541	0.92
237_V	256_L	0.540	0.92
82_T	98_G	0.540	0.92
33_G	193_Y	0.539	0.92
25_N	34_F	0.537	0.92
112_Y	116_G	0.536	0.92
247_K	251_T	0.536	0.92
105_D	141_T	0.535	0.92
142_I	163_D	0.534	0.92
106_T	133_H	0.532	0.92
91_T	219_G	0.532	0.92
243_Q	250_T	0.532	0.92
180_A	183_D	0.530	0.91
175_G	179_F	0.530	0.91
149_Q	161_I	0.529	0.91
174_I	177_V	0.528	0.91
118_F	129_G	0.528	0.91
9_I	15_F	0.527	0.91
167_K	170_D	0.526	0.91
153_I	218_S	0.525	0.91
253_D	257_Q	0.524	0.91
82_T	114_R	0.523	0.91
7_S	20_D	0.523	0.91
243_Q	246_S	0.522	0.91
242_F	255_I	0.520	0.91
102_F	153_I	0.518	0.91
146_T	163_D	0.517	0.90
175_G	180_A	0.516	0.90
159_V	171_G	0.512	0.90
39_V	76_I	0.511	0.90
43_D	68_G	0.511	0.90
189_G	192_L	0.510	0.90
236_I	239_Q	0.510	0.90
244_S	247_K	0.509	0.90
142_I	159_V	0.507	0.90
52_G	66_Q	0.506	0.90
141_T	146_T	0.505	0.89
135_L	175_G	0.505	0.89
210_D	215_A	0.505	0.89
121_D	124_G	0.505	0.89
246_S	259_L	0.503	0.89
32_V	86_Q	0.500	0.89
102_F	114_R	0.500	0.89

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3a69A	3	0.7083	100	0.103	Contact Map
1wlgA	2	0.5038	100	0.562	Contact Map
4ut1A	1	0.5189	99.7	0.725	Contact Map
1oryB	2	0.1515	15.9	0.958	Contact Map
2x5pA	1	0.3788	9.4	0.962	Contact Map
3j9aA	1	0.2159	4.5	0.967	Contact Map
2m4eA	1	0.072	2.6	0.971	Contact Map
2jujA	1	0.1629	1.8	0.973	Contact Map
4dwlA	3	0.1136	1.8	0.973	Contact Map
4g35B	1	0.0152	1.7	0.974	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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