GREMLIN Database
GPR - Germination protease
UniProt: P22322 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 368 (353)
Sequences: 153 (103)
Seq/√Len: 5.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
160_F240_V4.6700.97
108_S111_A4.3740.95
47_G89_K2.9000.72
138_V154_Y2.8380.70
115_I136_L2.7580.68
56_V302_L2.7120.66
92_A229_D2.6280.64
61_A180_I2.4990.60
209_S261_F2.4790.59
35_E188_V2.4400.58
82_N240_V2.4060.57
181_E184_K2.3490.55
68_E188_V2.3080.53
91_S360_Q2.2750.52
56_V240_V2.2540.51
180_I233_V2.1880.49
87_Q341_D2.1840.49
295_Q358_V2.1530.48
125_P193_A2.1460.48
72_L98_F2.0920.46
258_L302_L2.0840.46
40_I57_T2.0750.45
115_I257_I2.0710.45
56_V64_S2.0550.45
132_A191_I2.0340.44
94_F101_F1.9930.43
160_F179_V1.9910.42
143_F150_V1.9540.41
75_E80_R1.9510.41
57_T340_D1.9450.41
79_I174_D1.8260.37
98_F350_L1.8260.37
208_I350_L1.8250.37
257_I305_V1.7960.36
13_I228_K1.7920.36
247_V255_D1.7890.36
138_V240_V1.7490.35
238_I311_D1.7470.35
61_A154_Y1.7470.35
39_F52_R1.7210.34
51_I85_E1.7200.34
59_E62_E1.7190.34
196_A299_Q1.7180.34
127_A361_E1.7090.33
72_L101_F1.7080.33
248_T256_Y1.6970.33
305_V361_E1.6930.33
48_G284_K1.6880.33
128_L339_I1.6840.33
79_I93_V1.6800.33
76_A90_V1.6780.33
183_S208_I1.6680.32
113_C187_F1.6660.32
122_N134_E1.6610.32
310_E315_Q1.6410.31
39_F267_D1.6250.31
132_A179_V1.6240.31
103_E115_I1.6240.31
105_L236_I1.6210.31
63_L238_I1.6160.31
58_K68_E1.6160.31
49_I280_T1.6010.30
184_K228_K1.5980.30
259_K320_V1.5970.30
257_I315_Q1.5930.30
208_I286_V1.5920.30
135_N344_N1.5810.30
88_E345_V1.5810.30
31_V131_M1.5730.30
127_A132_A1.5710.29
99_S106_N1.5680.29
254_V320_V1.5650.29
204_T209_S1.5570.29
45_D358_V1.5550.29
144_K305_V1.5420.29
136_L240_V1.5390.29
301_F304_I1.5300.28
165_M196_A1.5240.28
94_F98_F1.5230.28
140_R208_I1.5180.28
289_E346_L1.5140.28
102_L348_N1.5100.28
115_I154_Y1.4930.27
31_V359_S1.4850.27
48_G133_V1.4790.27
59_E103_E1.4700.27
74_L146_Q1.4660.27
196_A248_T1.4590.26
152_E226_L1.4560.26
52_R161_A1.4460.26
32_P35_E1.4450.26
240_V345_V1.4400.26
145_L170_I1.4340.26
154_Y357_K1.4330.26
138_V248_T1.4320.26
78_G87_Q1.4270.26
90_V94_F1.4240.25
79_I259_K1.4170.25
208_I288_T1.4040.25
9_N90_V1.4000.25
31_V35_E1.3880.25
196_A357_K1.3850.24
82_N136_L1.3760.24
62_E266_K1.3670.24
67_K227_S1.3600.24
112_S151_Q1.3590.24
253_T327_N1.3540.24
52_R301_F1.3530.24
247_V250_A1.3440.23
78_G90_V1.3440.23
61_A68_E1.3280.23
133_V159_A1.3200.23
52_R318_H1.3120.23
84_S297_Q1.3100.23
76_A204_T1.3040.23
39_F352_T1.3000.22
32_P52_R1.2900.22
132_A346_L1.2850.22
36_I362_N1.2810.22
259_K327_N1.2800.22
155_R187_F1.2740.22
44_R256_Y1.2690.22
100_A115_I1.2660.22
11_Y302_L1.2640.22
105_L136_L1.2620.22
51_I191_I1.2560.21
62_E65_G1.2560.21
101_F208_I1.2520.21
305_V346_L1.2460.21
67_K253_T1.2330.21
63_L315_Q1.2310.21
154_Y248_T1.2280.21
240_V283_K1.2200.21
219_V253_T1.2160.20
67_K174_D1.2140.20
76_A346_L1.2100.20
32_P92_A1.2060.20
199_V286_V1.2040.20
61_A85_E1.2020.20
201_R205_T1.1970.20
198_A259_K1.1960.20
201_R244_V1.1890.20
240_V301_F1.1850.20
17_L20_E1.1730.20
19_V177_K1.1640.19
32_P85_E1.1620.19
36_I50_K1.1590.19
161_A289_E1.1550.19
59_E275_V1.1500.19
247_V329_M1.1480.19
233_V359_S1.1470.19
23_E106_N1.1450.19
264_E342_M1.1450.19
38_G197_R1.1430.19
111_A146_Q1.1410.19
39_F64_S1.1390.19
250_A329_M1.1300.19
61_A211_T1.1300.19
115_I310_E1.1280.19
31_V89_K1.1220.18
34_K115_I1.1210.18
49_I175_I1.1170.18
140_R144_K1.1170.18
94_F211_T1.1120.18
116_V178_G1.1120.18
28_Q110_D1.1120.18
187_F354_L1.1110.18
74_L240_V1.1060.18
160_F182_Q1.1060.18
128_L157_V1.1050.18
264_E287_L1.1010.18
12_L25_M1.1000.18
51_I238_I1.0960.18
35_E348_N1.0840.18
75_E191_I1.0810.18
175_I179_V1.0790.18
257_I296_K1.0770.18
77_Q180_I1.0720.17
55_D191_I1.0680.17
37_K74_L1.0680.17
88_E108_S1.0620.17
98_F101_F1.0570.17
23_E33_T1.0560.17
78_G123_V1.0540.17
136_L154_Y1.0520.17
25_M94_F1.0470.17
155_R355_H1.0470.17
121_W204_T1.0420.17
259_K329_M1.0390.17
294_D297_Q1.0380.17
64_S345_V1.0310.17
13_I226_L1.0260.17
250_A255_D1.0220.17
57_T309_Q1.0090.16
102_L226_L1.0040.16
37_K63_L1.0040.16
40_I339_I0.9980.16
98_F226_L0.9970.16
35_E41_E0.9960.16
270_P329_M0.9920.16
45_D48_G0.9910.16
306_G327_N0.9910.16
295_Q299_Q0.9760.16
115_I138_V0.9700.16
142_L152_E0.9680.16
78_G86_M0.9670.16
76_A180_I0.9630.15
228_K284_K0.9580.15
90_V132_A0.9570.15
127_A253_T0.9560.15
160_F294_D0.9560.15
34_K52_R0.9560.15
244_V252_D0.9540.15
146_Q196_A0.9530.15
98_F132_A0.9520.15
91_S101_F0.9490.15
144_K294_D0.9490.15
195_A244_V0.9480.15
144_K152_E0.9460.15
40_I261_F0.9450.15
28_Q37_K0.9440.15
31_V136_L0.9440.15
78_G341_D0.9410.15
103_E109_K0.9390.15
16_D20_E0.9380.15
19_V180_I0.9380.15
180_I226_L0.9370.15
289_E297_Q0.9290.15
64_S302_L0.9280.15
56_V335_V0.9270.15
32_P286_V0.9230.15
155_R354_L0.9220.15
31_V55_D0.9160.15
191_I357_K0.9120.15
275_V279_M0.9070.14
31_V85_E0.9040.14
10_Q177_K0.9010.14
258_L340_D0.9000.14
31_V309_Q0.9000.14
261_F329_M0.9000.14
93_V176_I0.8970.14
290_D299_Q0.8920.14
26_A146_Q0.8860.14
56_V211_T0.8840.14
147_P204_T0.8770.14
190_A345_V0.8700.14
88_E180_I0.8690.14
56_V138_V0.8670.14
121_W134_E0.8650.14
101_F167_I0.8640.14
37_K266_K0.8630.14
38_G60_G0.8610.14
48_G86_M0.8600.14
322_S360_Q0.8570.14
83_D275_V0.8530.14
166_G328_L0.8530.14
240_V356_E0.8490.14
70_R91_S0.8480.14
50_K97_E0.8450.13
31_V144_K0.8380.13
153_G205_T0.8370.13
132_A301_F0.8360.13
278_G326_H0.8350.13
102_L261_F0.8350.13
14_R17_L0.8300.13
34_K361_E0.8290.13
135_N340_D0.8250.13
31_V45_D0.8250.13
91_S356_E0.8210.13
31_V361_E0.8190.13
337_S340_D0.8150.13
144_K357_K0.8150.13
25_M140_R0.8120.13
338_F358_V0.8120.13
75_E158_S0.8070.13
75_E322_S0.8020.13
11_Y236_I0.8010.13
101_F140_R0.7990.13
229_D290_D0.7970.13
84_S229_D0.7940.13
64_S229_D0.7930.13
62_E70_R0.7870.13
52_R57_T0.7870.13
161_A343_A0.7850.13
22_K32_P0.7820.12
245_D329_M0.7780.12
225_D273_S0.7750.12
40_I360_Q0.7750.12
55_D305_V0.7750.12
94_F349_G0.7730.12
64_S144_K0.7730.12
286_V289_E0.7720.12
198_A326_H0.7720.12
196_A285_K0.7710.12
77_Q165_M0.7700.12
294_D357_K0.7680.12
159_A186_D0.7680.12
131_M339_I0.7590.12
29_Q74_L0.7590.12
40_I257_I0.7570.12
59_E358_V0.7570.12
39_F359_S0.7560.12
180_I188_V0.7550.12
35_E104_N0.7550.12
24_A39_F0.7540.12
46_H111_A0.7480.12
56_V82_N0.7470.12
12_L356_E0.7460.12
137_L358_V0.7450.12
109_K356_E0.7450.12
51_I348_N0.7450.12
105_L135_N0.7430.12
189_I302_L0.7420.12
97_E208_I0.7410.12
151_Q307_T0.7410.12
135_N208_I0.7400.12
67_K176_I0.7400.12
142_L327_N0.7390.12
271_S358_V0.7380.12
188_V345_V0.7350.12
253_T263_R0.7330.12
309_Q346_L0.7290.12
46_H63_L0.7290.12
19_V346_L0.7260.12
46_H115_I0.7250.12
76_A81_E0.7240.12
337_S341_D0.7230.12
331_T349_G0.7220.12
222_K254_V0.7190.12
310_E359_S0.7180.12
63_L348_N0.7170.12
91_S337_S0.7170.12
67_K225_D0.7150.12
42_K261_F0.7130.12
133_V208_I0.7120.11
136_L279_M0.7100.11
11_Y105_L0.7070.11
52_R283_K0.7060.11
94_F208_I0.7050.11
327_N351_N0.7030.11
227_S259_K0.7030.11
225_D309_Q0.7020.11
156_P266_K0.7020.11
180_I357_K0.6980.11
59_E294_D0.6960.11
82_N87_Q0.6940.11
106_N238_I0.6920.11
264_E276_P0.6920.11
35_E64_S0.6910.11
103_E359_S0.6890.11
90_V339_I0.6860.11
227_S271_S0.6860.11
195_A219_V0.6850.11
64_S240_V0.6840.11
52_R302_L0.6830.11
55_D362_N0.6780.11
251_S329_M0.6780.11
199_V342_M0.6770.11
45_D361_E0.6770.11
9_N138_V0.6760.11
98_F322_S0.6750.11
84_S92_A0.6740.11
148_E277_A0.6730.11
257_I301_F0.6730.11
45_D108_S0.6700.11
29_Q238_I0.6670.11
81_E146_Q0.6670.11
64_S190_A0.6670.11
79_I198_A0.6660.11
224_K313_K0.6660.11
56_V263_R0.6660.11
79_I309_Q0.6650.11
209_S262_G0.6650.11
74_L315_Q0.6640.11
50_K345_V0.6610.11
147_P293_P0.6600.11
32_P42_K0.6580.11
74_L296_K0.6550.11
74_L92_A0.6540.11
250_A259_K0.6530.11
102_L295_Q0.6520.11
92_A285_K0.6480.11
138_V206_I0.6480.11
9_N318_H0.6470.11
25_M229_D0.6450.11
34_K340_D0.6450.11
256_Y311_D0.6450.11
65_G326_H0.6420.11
106_N115_I0.6410.11
59_E108_S0.6410.11
45_D50_K0.6400.11
134_E261_F0.6390.11
10_Q70_R0.6350.10
196_A199_V0.6350.10
283_K348_N0.6330.10
178_G248_T0.6330.10
87_Q200_E0.6320.10
34_K315_Q0.6320.10
49_I134_E0.6310.10
139_T308_L0.6310.10
74_L324_L0.6310.10
307_T339_I0.6290.10
166_G330_V0.6280.10
86_M350_L0.6280.10
106_N176_I0.6260.10
26_A32_P0.6250.10
109_K359_S0.6230.10
9_N167_I0.6230.10
72_L211_T0.6220.10
257_I311_D0.6210.10
175_I305_V0.6200.10
146_Q302_L0.6190.10
153_G331_T0.6190.10
107_I349_G0.6160.10
96_E269_R0.6120.10
202_V224_K0.6120.10
123_V197_R0.6100.10
222_K328_L0.6090.10
45_D85_E0.6080.10
318_H324_L0.6080.10
143_F157_V0.6060.10
31_V34_K0.6040.10
61_A231_L0.6040.10
28_Q31_V0.6030.10
219_V232_G0.5970.10
272_R277_A0.5970.10
61_A273_S0.5960.10
196_A294_D0.5940.10
101_F135_N0.5930.10
77_Q250_A0.5920.10
66_K245_D0.5900.10
15_T205_T0.5870.10
253_T259_K0.5860.10
88_E238_I0.5860.10
92_A296_K0.5850.10
35_E99_S0.5840.10
220_G243_V0.5830.10
35_E268_N0.5810.10
37_K47_G0.5800.10
335_V349_G0.5800.10
84_S250_A0.5790.10
157_V355_H0.5790.10
51_I107_I0.5770.10
76_A86_M0.5770.10
200_E256_Y0.5760.10
32_P196_A0.5750.10
11_Y112_S0.5740.10
240_V289_E0.5740.10
81_E284_K0.5730.10
338_F342_M0.5720.10
72_L307_T0.5710.10
90_V167_I0.5710.10
135_N337_S0.5710.10
94_F161_A0.5710.10
116_V339_I0.5700.10
135_N227_S0.5680.10
79_I350_L0.5670.10
80_R204_T0.5660.10
177_K191_I0.5650.10
227_S329_M0.5650.10
208_I265_M0.5630.10
63_L361_E0.5630.10
154_Y287_L0.5620.10
63_L103_E0.5620.10
88_E362_N0.5560.10
158_S183_S0.5550.09
92_A138_V0.5540.09
150_V155_R0.5540.09
91_S352_T0.5530.09
249_I330_V0.5530.09
42_K53_T0.5520.09
28_Q49_I0.5520.09
188_V228_K0.5510.09
12_L161_A0.5510.09
225_D358_V0.5490.09
131_M343_A0.5490.09
301_F344_N0.5480.09
139_T306_G0.5480.09
153_G355_H0.5470.09
58_K345_V0.5460.09
84_S352_T0.5450.09
258_L265_M0.5450.09
114_L232_G0.5420.09
85_E191_I0.5410.09
313_K335_V0.5390.09
21_T293_P0.5380.09
238_I315_Q0.5370.09
107_I228_K0.5340.09
111_A283_K0.5340.09
35_E46_H0.5310.09
140_R158_S0.5300.09
41_E70_R0.5300.09
135_N339_I0.5280.09
59_E238_I0.5270.09
67_K259_K0.5270.09
57_T318_H0.5270.09
262_G327_N0.5260.09
200_E251_S0.5260.09
248_T325_G0.5250.09
72_L81_E0.5240.09
259_K323_P0.5230.09
247_V270_P0.5230.09
94_F202_V0.5230.09
293_P313_K0.5220.09
115_I275_V0.5220.09
41_E326_H0.5220.09
148_E349_G0.5200.09
306_G351_N0.5190.09
54_V255_D0.5160.09
44_R265_M0.5150.09
21_T24_A0.5150.09
64_S132_A0.5150.09
68_E228_K0.5140.09
285_K322_S0.5130.09
130_P333_K0.5120.09
300_S309_Q0.5120.09
94_F350_L0.5110.09
14_R20_E0.5110.09
96_E289_E0.5110.09
161_A273_S0.5110.09
70_R280_T0.5070.09
37_K268_N0.5070.09
34_K238_I0.5070.09
50_K190_A0.5060.09
255_D281_F0.5060.09
11_Y101_F0.5050.09
31_V357_K0.5040.09
44_R116_V0.5020.09
46_H96_E0.5010.09
156_P335_V0.5000.09
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1c8bA 3 0.8587 100 0.264 Contact Map
1cfzA 1 0.3668 95.9 0.963 Contact Map
2e85A 2 0.3886 92.1 0.969 Contact Map
3pu6A 1 0.3587 91.7 0.969 Contact Map
2jrtA 1 0.2391 38.3 0.98 Contact Map
2xdqB 2 0.5707 34.5 0.981 Contact Map
3aa0A 1 0.1522 32.3 0.981 Contact Map
1up7A 3 0.5217 28.9 0.982 Contact Map
3ff4A 1 0.2962 28.3 0.982 Contact Map
2h2mA 1 0.1984 27.5 0.982 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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