GREMLIN Database
HEM4 - Uroporphyrinogen-III synthase
UniProt: P21248 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 262 (240)
Sequences: 4252 (3228)
Seq/√Len: 208.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
28_V33_G4.5121.00
221_S227_N4.0831.00
62_P161_K3.7441.00
143_N167_E3.5821.00
195_S229_A3.1801.00
227_N239_T2.7751.00
136_T159_E2.4851.00
64_W94_A2.4741.00
141_K162_E2.4511.00
152_E155_P2.4311.00
62_P138_T2.4151.00
189_Y220_I2.3461.00
11_T34_K2.3381.00
95_A101_A2.3041.00
44_R169_I2.2651.00
199_H229_A2.2411.00
27_K31_L2.2311.00
44_R167_E2.2201.00
77_F107_H2.1101.00
63_G135_E2.0701.00
198_V230_L2.0511.00
171_D200_T2.0231.00
124_A152_E1.9691.00
25_Q37_L1.9561.00
53_E79_Y1.9521.00
221_S230_L1.9311.00
221_S239_T1.9291.00
67_F73_A1.9061.00
219_C235_I1.9011.00
42_T171_D1.8841.00
189_Y252_M1.8631.00
93_I109_V1.8551.00
27_K246_D1.8331.00
14_V25_Q1.8231.00
13_L187_F1.8051.00
113_V126_A1.8021.00
74_D103_R1.7991.00
128_K156_L1.7471.00
64_W92_K1.6971.00
141_K145_S1.6791.00
14_V21_A1.6641.00
228_D231_L1.6541.00
228_D232_T1.6501.00
43_F168_T1.6401.00
120_A149_I1.6381.00
25_Q35_A1.6191.00
202_M233_Y1.6161.00
227_N231_L1.6091.00
43_F71_N1.6061.00
67_F76_F1.6051.00
64_W137_I1.5961.00
20_Q71_N1.5741.00
150_K154_V1.5531.00
136_T161_K1.5521.00
103_R106_M1.5291.00
119_I122_Q1.5261.00
147_D151_Q1.5091.00
94_A123_L1.4861.00
121_E146_R1.4841.00
31_L253_C1.4821.00
192_F201_F1.4821.00
199_H233_Y1.4741.00
45_R75_F1.4711.00
220_I248_M1.4681.00
243_F247_G1.4591.00
117_E122_Q1.4471.00
75_F165_L1.4401.00
230_L237_S1.4321.00
151_Q154_V1.3751.00
240_P247_G1.3501.00
63_G136_T1.3281.00
116_Q126_A1.3271.00
191_T248_M1.3251.00
193_T197_T1.3191.00
28_V35_A1.3161.00
150_K162_E1.3151.00
242_T247_G1.3031.00
70_V99_K1.3021.00
192_F198_V1.2981.00
222_I226_T1.2931.00
42_T169_I1.2861.00
61_A136_T1.2801.00
180_D184_Q1.2611.00
223_G226_T1.2601.00
11_T36_V1.2601.00
168_T196_S1.2561.00
29_E35_A1.2561.00
219_C230_L1.2401.00
229_A232_T1.2371.00
95_A104_L1.2221.00
143_N164_V1.2221.00
230_L235_I1.2111.00
192_F219_C1.1991.00
26_Q30_A1.1901.00
92_K112_D1.1851.00
25_Q29_E1.1811.00
189_Y218_A1.1761.00
224_P243_F1.1481.00
95_A114_M1.1481.00
132_E135_E1.1351.00
58_D61_A1.1311.00
170_P203_H1.1291.00
26_Q29_E1.1131.00
101_A105_K1.1101.00
139_V150_K1.1051.00
195_S225_L1.0761.00
244_T247_G1.0611.00
229_A233_Y1.0531.00
54_Q58_D1.0511.00
66_V127_L1.0451.00
40_L200_T1.0421.00
120_A124_A1.0251.00
170_P200_T1.0101.00
179_K183_G1.0061.00
64_W113_V1.0051.00
58_D161_K1.0041.00
18_K22_A1.0031.00
144_L196_S0.9991.00
101_A114_M0.9871.00
73_A103_R0.9871.00
70_V103_R0.9851.00
202_M235_I0.9731.00
105_K110_S0.9731.00
176_E180_D0.9691.00
22_A37_L0.9681.00
137_I158_F0.9611.00
65_L140_M0.9591.00
65_L93_I0.9520.99
181_A186_S0.9480.99
73_A100_T0.9330.99
121_E148_V0.9310.99
148_V152_E0.9270.99
36_V187_F0.9250.99
54_Q57_E0.9250.99
53_E57_E0.9080.99
27_K30_A0.9070.99
72_G140_M0.9070.99
144_L168_T0.9030.99
24_F191_T0.9020.99
16_R197_T0.9010.99
18_K21_A0.9010.99
152_E156_L0.8980.99
42_T172_E0.8940.99
14_V191_T0.8920.99
113_V123_L0.8910.99
145_S162_E0.8900.99
105_K111_V0.8890.99
65_L91_K0.8830.99
12_V189_Y0.8720.99
96_V120_A0.8690.99
95_A111_V0.8680.99
195_S226_T0.8630.99
200_T204_V0.8630.99
220_I251_L0.8590.99
41_I200_T0.8560.99
14_V37_L0.8530.99
46_A164_V0.8510.99
66_V123_L0.8470.99
224_P228_D0.8460.99
134_G158_F0.8400.99
154_V160_V0.8390.99
182_A213_K0.8380.99
36_V181_A0.8340.99
72_G139_V0.8290.99
43_F75_F0.8290.99
46_A167_E0.8250.99
120_A148_V0.8240.99
102_R106_M0.8180.98
22_A26_Q0.8170.98
98_E118_Y0.8150.98
191_T220_I0.8150.98
240_P251_L0.8100.98
71_N75_F0.8090.98
74_D78_S0.8070.98
140_M165_L0.8040.98
76_F140_M0.8030.98
119_I224_P0.8010.98
41_I168_T0.7970.98
40_L201_F0.7970.98
191_T252_M0.7940.98
124_A156_L0.7940.98
231_L236_T0.7890.98
138_T163_W0.7890.98
21_A37_L0.7850.98
190_V219_C0.7850.98
14_V28_V0.7850.98
40_L204_V0.7830.98
151_Q155_P0.7780.98
109_V112_D0.7700.98
41_I194_S0.7680.98
206_G209_L0.7620.98
132_E158_F0.7620.98
24_F28_V0.7610.98
14_V35_A0.7600.98
219_C238_H0.7600.98
218_A238_H0.7550.97
74_D106_M0.7530.97
241_D250_E0.7510.97
120_A168_T0.7490.97
104_L109_V0.7470.97
78_S107_H0.7400.97
126_A130_H0.7390.97
11_T188_D0.7380.97
99_K102_R0.7240.97
64_W127_L0.7210.97
139_V149_I0.7210.97
153_L158_F0.7150.96
142_G164_V0.7120.96
147_D162_E0.7100.96
139_V160_V0.7050.96
148_V151_Q0.7050.96
103_R107_H0.7050.96
118_Y244_T0.7040.96
141_K150_K0.7020.96
46_A49_N0.6980.96
67_F93_I0.6960.96
52_A79_Y0.6920.96
115_P118_Y0.6920.96
193_T245_I0.6900.96
123_L127_L0.6900.96
146_R225_L0.6870.95
224_P239_T0.6840.95
185_Y216_G0.6810.95
76_F165_L0.6800.95
92_K130_H0.6790.95
94_A126_A0.6790.95
81_K85_L0.6770.95
45_R78_S0.6750.95
66_V149_I0.6740.95
128_K152_E0.6740.95
102_R105_K0.6730.95
15_T197_T0.6720.95
67_F140_M0.6710.95
18_K37_L0.6690.95
16_R100_T0.6670.95
230_L234_G0.6670.95
250_E254_S0.6640.95
96_V123_L0.6640.95
75_F78_S0.6620.95
173_E204_V0.6620.95
118_Y122_Q0.6580.94
177_A181_A0.6580.94
51_V54_Q0.6570.94
38_T173_E0.6570.94
247_G251_L0.6490.94
142_G196_S0.6490.94
57_E83_N0.6480.94
94_A193_T0.6460.94
196_S223_G0.6450.94
93_I104_L0.6400.93
249_L252_M0.6380.93
49_N53_E0.6380.93
17_N43_F0.6380.93
63_G90_H0.6350.93
225_L243_F0.6320.93
52_A165_L0.6310.93
129_Q156_L0.6290.93
53_E82_E0.6260.93
41_I196_S0.6240.93
238_H251_L0.6240.93
180_D183_G0.6220.92
96_V100_T0.6220.92
144_L164_V0.6220.92
52_A163_W0.6220.92
24_F193_T0.6120.92
119_I243_F0.6110.92
231_L237_S0.6090.92
141_K147_D0.6080.92
249_L253_C0.6060.91
51_V55_V0.6050.91
20_Q70_V0.6040.91
190_V217_T0.6010.91
67_F95_A0.6010.91
94_A115_P0.6000.91
53_E60_A0.5980.91
67_F100_T0.5950.91
64_W126_A0.5920.91
120_A145_S0.5920.91
120_A225_L0.5920.91
222_I240_P0.5900.90
53_E83_N0.5880.90
96_V142_G0.5880.90
154_V159_E0.5870.90
119_I225_L0.5850.90
33_G253_C0.5840.90
27_K249_L0.5820.90
64_W135_E0.5810.90
241_D254_S0.5800.90
250_E253_C0.5790.90
16_R20_Q0.5770.89
41_I197_T0.5760.89
124_A148_V0.5760.89
101_A111_V0.5760.89
23_S26_Q0.5730.89
60_A85_L0.5720.89
71_N74_D0.5690.89
142_G165_L0.5690.89
199_H232_T0.5680.89
29_E34_K0.5680.89
124_A149_I0.5670.89
66_V158_F0.5660.88
142_G168_T0.5650.88
67_F139_V0.5640.88
66_V94_A0.5640.88
55_V161_K0.5620.88
127_L130_H0.5620.88
69_S96_V0.5610.88
73_A104_L0.5590.88
139_V193_T0.5580.88
192_F197_T0.5570.88
140_M145_S0.5530.87
98_E244_T0.5520.87
108_N111_V0.5520.87
182_A187_F0.5520.87
122_Q126_A0.5510.87
65_L138_T0.5500.87
141_K164_V0.5500.87
69_S100_T0.5470.87
240_P243_F0.5460.87
140_M163_W0.5460.87
241_D251_L0.5440.86
124_A127_L0.5410.86
139_V144_L0.5390.86
122_Q125_D0.5380.86
15_T39_S0.5380.86
40_L192_F0.5340.85
14_V24_F0.5340.85
124_A128_K0.5340.85
127_L156_L0.5340.85
20_Q24_F0.5330.85
179_K208_E0.5320.85
171_D174_G0.5310.85
135_E158_F0.5300.85
38_T178_L0.5240.84
147_D150_K0.5240.84
231_L239_T0.5220.84
220_I252_M0.5220.84
125_D152_E0.5220.84
12_V15_T0.5210.84
29_E37_L0.5210.84
146_R168_T0.5200.84
68_T168_T0.5190.84
139_V162_E0.5180.84
79_Y82_E0.5170.84
144_L199_H0.5160.84
220_I224_P0.5160.84
69_S142_G0.5130.83
170_P204_V0.5120.83
99_K240_P0.5110.83
203_H206_G0.5090.83
57_E85_L0.5090.83
92_K135_E0.5080.83
24_F249_L0.5080.83
191_T245_I0.5060.82
139_V145_S0.5050.82
131_A135_E0.5040.82
15_T24_F0.5020.82
199_H203_H0.5010.82
220_I238_H0.5010.82
145_S225_L0.5000.82
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3re1A 1 0.9618 100 0.17 Contact Map
3d8tA 1 0.9313 100 0.179 Contact Map
1wcwA 1 0.9389 100 0.181 Contact Map
1jr2A 1 0.9313 100 0.196 Contact Map
4es6A 1 0.9313 100 0.196 Contact Map
3mw8A 1 0.8969 100 0.222 Contact Map
3p9zA 1 0.8092 100 0.311 Contact Map
2vk2A 1 0.9237 96 0.891 Contact Map
2o20A 2 0.9008 95.9 0.891 Contact Map
3qk7A 2 0.8893 95.9 0.891 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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