GREMLIN Database
PPB4 - Alkaline phosphatase 4
UniProt: P19406 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 461 (403)
Sequences: 1912 (1291)
Seq/√Len: 64.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
209_R214_E5.0291.00
225_T228_A4.2651.00
210_N213_K4.0591.00
247_A256_T3.8581.00
310_K314_E3.5871.00
226_K230_K3.3751.00
148_S273_N3.2671.00
264_V311_A3.2641.00
226_K261_D3.1471.00
136_K144_Q3.0901.00
285_Q288_W3.0481.00
98_P110_A2.8021.00
157_I196_L2.7191.00
158_N175_M2.7181.00
166_G182_Y2.6671.00
143_K194_D2.6401.00
106_T111_A2.6221.00
156_E175_M2.5941.00
139_L278_F2.5441.00
167_A178_I2.5351.00
260_K311_A2.4411.00
265_S268_D2.4161.00
155_S284_S2.3981.00
265_S269_R2.3941.00
212_T222_Y2.3721.00
94_M430_V2.3421.00
260_K307_Q2.3311.00
161_T280_M2.3201.00
153_A284_S2.3091.00
308_A312_A2.3091.00
197_L237_V2.3071.00
197_L239_G2.2491.00
260_K304_D2.1971.00
112_G164_A2.1831.00
57_G286_I2.1781.00
305_F309_Y2.1741.00
283_G305_F2.1281.00
252_R258_S2.0431.00
137_S140_E2.0221.00
249_A258_S2.0121.00
304_D307_Q1.9981.00
201_K222_Y1.9851.00
249_A304_D1.9751.00
68_Y303_K1.9521.00
85_E306_E1.9471.00
63_P426_E1.9301.00
268_D315_F1.8691.00
61_G331_T1.8631.00
125_A128_V1.8531.00
139_L149_T1.8161.00
267_I312_A1.8001.00
176_D203_Y1.7931.00
180_N184_D1.7931.00
84_T310_K1.7891.00
68_Y302_V1.7260.99
251_D297_G1.7070.99
169_N181_S1.7000.99
55_M312_A1.7000.99
56_I115_L1.6990.99
84_T306_E1.6930.99
92_G430_V1.6880.99
121_T125_A1.6870.99
263_T312_A1.6770.99
92_G444_I1.6690.99
65_I431_Y1.6650.99
311_A314_E1.6530.99
55_M305_F1.6410.99
98_P123_N1.6310.99
180_N209_R1.5900.99
127_G164_A1.5850.99
325_I440_F1.5840.99
53_I312_A1.5720.99
61_G328_A1.5710.99
117_T127_G1.5650.99
140_E168_H1.5650.99
72_K81_P1.5630.99
294_D297_G1.5570.99
126_I163_A1.5530.99
266_A279_L1.5370.99
141_E450_A1.5350.99
122_Y125_A1.5210.99
386_E413_R1.5070.99
147_K457_L1.5010.99
151_L165_Y1.4910.98
138_V450_A1.4800.98
138_V453_I1.4690.98
263_T281_V1.4640.98
301_E304_D1.4480.98
99_D122_Y1.4440.98
94_M449_Q1.4440.98
49_I456_I1.4430.98
158_N179_A1.4370.98
430_V444_I1.4290.98
249_A252_R1.4280.98
64_Y290_A1.4260.98
92_G441_R1.4250.98
331_T426_E1.4250.98
260_K308_A1.4210.98
264_V268_D1.4000.98
316_A324_V1.3980.98
448_D451_N1.3950.98
55_M324_V1.3940.98
317_K320_K1.3930.98
62_T429_P1.3880.97
237_V269_R1.3870.97
290_A298_A1.3810.97
142_A457_L1.3790.97
375_A379_N1.3740.97
220_Y236_Q1.3720.97
117_T135_V1.3670.97
106_T110_A1.3650.97
316_A322_T1.3520.97
248_K285_Q1.3510.97
94_M444_I1.3340.97
126_I169_N1.3340.97
316_A433_Y1.3270.97
383_T386_E1.3190.97
197_L241_F1.3130.96
103_Y123_N1.2950.96
115_L449_Q1.2920.96
156_E244_G1.2910.96
127_G137_S1.2870.96
95_M445_N1.2850.96
202_S205_N1.2830.96
175_M245_G1.2830.96
183_M215_F1.2780.96
313_I433_Y1.2760.96
72_K306_E1.2750.96
52_V457_L1.2740.96
116_A165_Y1.2710.96
55_M283_G1.2660.96
98_P106_T1.2650.96
129_D133_K1.2650.96
202_S244_G1.2620.96
242_A256_T1.2600.96
377_Y381_K1.2570.95
95_M443_L1.2560.95
159_H245_G1.2490.95
99_D130_K1.2430.95
61_G426_E1.2430.95
112_G116_A1.2430.95
156_E245_G1.2400.95
150_G279_L1.2360.95
430_V449_Q1.2350.95
56_I161_T1.2250.95
155_S288_W1.2180.95
248_K292_D1.2180.95
239_G262_M1.2160.95
151_L166_G1.2100.94
440_F452_I1.1980.94
308_A311_A1.1930.94
139_L193_I1.1870.94
439_K455_K1.1850.94
72_K303_K1.1780.94
229_L265_S1.1690.93
213_K216_K1.1630.93
69_R427_E1.1610.93
127_G135_V1.1600.93
68_Y83_L1.1540.93
194_D236_Q1.1540.93
286_I302_V1.1480.93
375_A378_A1.1410.92
325_I453_I1.1390.92
97_H445_N1.1380.92
55_M309_Y1.1370.92
106_T123_N1.1370.92
448_D452_I1.1340.92
215_F238_L1.1250.92
313_I324_V1.1230.92
246_L262_M1.1220.92
159_H288_W1.1200.92
174_N177_Q1.1120.92
283_G308_A1.1020.91
156_E159_H1.1010.91
305_F326_A1.0970.91
195_V266_A1.0880.91
245_G288_W1.0650.90
386_E389_S1.0600.89
110_A123_N1.0560.89
53_I316_A1.0560.89
106_T446_N1.0550.89
263_T267_I1.0540.89
142_A459_T1.0520.89
52_V453_I1.0500.89
201_K224_T1.0460.89
312_A316_A1.0420.89
363_K366_E1.0420.89
139_L165_Y1.0390.88
54_V278_F1.0390.88
57_G327_T1.0380.88
242_A247_A1.0370.88
224_T228_A1.0340.88
60_M65_I1.0330.88
116_A127_G1.0290.88
245_G285_Q1.0290.88
90_L437_K1.0280.88
259_L304_D1.0260.88
246_L256_T1.0250.88
379_N382_V1.0210.88
311_A315_F1.0210.88
230_K265_S1.0150.87
283_G312_A1.0110.87
143_K149_T1.0080.87
142_A149_T0.9920.86
84_T87_D0.9880.86
141_E454_F0.9830.86
241_F262_M0.9780.85
431_Y443_L0.9750.85
88_R433_Y0.9740.85
450_A454_F0.9720.85
115_L280_M0.9680.85
235_Q272_Q0.9640.84
124_N171_S0.9630.84
158_N204_F0.9580.84
375_A381_K0.9570.84
152_V195_V0.9560.84
250_L297_G0.9540.84
202_S243_D0.9530.84
159_H175_M0.9510.84
331_T424_T0.9500.84
152_V266_A0.9440.83
82_K87_D0.9430.83
312_A324_V0.9430.83
140_E144_Q0.9380.83
84_T313_I0.9370.83
136_K141_E0.9370.83
444_I448_D0.9360.83
82_K86_F0.9360.83
158_N245_G0.9330.82
284_S288_W0.9290.82
250_L356_T0.9220.82
71_M303_K0.9210.82
250_L296_V0.9180.81
80_N83_L0.9170.81
66_R70_S0.9140.81
318_K321_H0.9130.81
316_A320_K0.9090.81
260_K264_V0.9090.81
248_K289_A0.9080.81
122_Y130_K0.9070.81
296_V408_K0.9030.80
54_V325_I0.9020.80
388_K392_A0.9010.80
377_Y382_V0.8980.80
222_Y240_L0.8970.80
69_R86_F0.8930.80
126_I164_A0.8890.79
183_M214_E0.8860.79
143_K192_K0.8800.79
274_K320_K0.8770.78
197_L229_L0.8740.78
150_G276_G0.8730.78
105_I338_G0.8720.78
60_M302_V0.8720.78
61_G335_F0.8710.78
54_V327_T0.8710.78
182_Y215_F0.8640.77
324_V433_Y0.8620.77
289_A294_D0.8540.76
267_I315_F0.8540.76
234_D269_R0.8520.76
201_K243_D0.8500.76
372_D376_R0.8500.76
90_L441_R0.8500.76
158_N203_Y0.8500.76
213_K217_Q0.8470.76
93_M429_P0.8450.76
135_V168_H0.8380.75
317_K321_H0.8350.75
150_G195_V0.8320.75
146_G275_K0.8280.74
148_S275_K0.8250.74
197_L262_M0.8240.74
195_V235_Q0.8240.74
148_S194_D0.8230.74
57_G60_M0.8230.74
304_D308_A0.8180.73
175_M244_G0.8180.73
151_L278_F0.8170.73
317_K433_Y0.8160.73
195_V237_V0.8160.73
449_Q453_I0.8160.73
66_R337_I0.8140.73
306_E310_K0.8130.73
143_K191_H0.8100.73
67_A299_M0.8080.72
137_S168_H0.8080.72
381_K387_I0.8060.72
157_I182_Y0.8050.72
147_K276_G0.8040.72
372_D377_Y0.8020.72
111_A446_N0.8020.72
212_T224_T0.8010.72
55_M281_V0.8010.72
227_Q230_K0.7990.72
230_K261_D0.7990.72
271_N315_F0.7990.72
296_V300_S0.7980.71
78_P86_F0.7950.71
54_V115_L0.7950.71
248_K288_W0.7930.71
159_H173_K0.7900.71
116_A280_M0.7900.71
314_E318_K0.7810.70
289_A297_G0.7810.70
251_D301_E0.7780.69
83_L86_F0.7760.69
309_Y326_A0.7760.69
109_A126_I0.7760.69
303_K306_E0.7730.69
71_M300_S0.7700.69
197_L223_V0.7680.69
307_Q310_K0.7660.68
211_L214_E0.7650.68
223_V237_V0.7630.68
399_S404_K0.7630.68
407_I411_N0.7630.68
119_V135_V0.7620.68
134_K168_H0.7580.67
370_V374_L0.7580.67
444_I452_I0.7570.67
182_Y196_L0.7560.67
57_G328_A0.7560.67
314_E317_K0.7550.67
74_N81_P0.7550.67
85_E88_R0.7540.67
281_V308_A0.7520.67
74_N78_P0.7490.67
376_R379_N0.7490.67
61_G98_P0.7480.66
212_T238_L0.7480.66
65_I429_P0.7460.66
206_R210_N0.7450.66
303_K307_Q0.7440.66
432_A440_F0.7430.66
60_M286_I0.7420.66
91_T432_A0.7410.66
50_R321_H0.7380.65
223_V229_L0.7370.65
73_N81_P0.7350.65
227_Q255_K0.7340.65
55_M147_K0.7330.65
323_L437_K0.7330.65
229_L239_G0.7320.65
378_A381_K0.7320.65
94_M325_I0.7310.65
96_T428_V0.7300.65
212_T240_L0.7300.65
142_A147_K0.7300.65
156_E285_Q0.7270.64
62_T426_E0.7250.64
214_E217_Q0.7210.64
85_E431_Y0.7200.63
180_N211_L0.7200.63
73_N79_N0.7170.63
264_V315_F0.7170.63
216_K219_G0.7150.63
277_F315_F0.7140.63
236_Q272_Q0.7140.63
151_L193_I0.7130.63
325_I450_A0.7110.63
205_N208_D0.7100.62
116_A278_F0.7090.62
368_K371_K0.7070.62
293_N334_G0.7060.62
359_F363_K0.7060.62
154_T262_M0.7060.62
64_Y331_T0.7050.62
62_T93_M0.7040.62
154_T285_Q0.7030.62
102_D122_Y0.7030.62
367_G371_K0.7020.62
78_P84_T0.7000.61
382_V386_E0.6980.61
434_G437_K0.6980.61
348_E372_D0.6980.61
197_L279_L0.6970.61
229_L256_T0.6970.61
387_I391_E0.6960.61
334_G338_G0.6960.61
105_I123_N0.6950.61
60_M327_T0.6940.61
222_Y261_D0.6930.61
292_D420_S0.6920.60
369_P372_D0.6870.60
347_A350_I0.6860.60
169_N174_N0.6860.60
115_L450_A0.6850.60
88_R313_I0.6850.60
77_T81_P0.6830.59
216_K222_Y0.6820.59
411_N418_W0.6790.59
318_K433_Y0.6790.59
66_R86_F0.6770.59
251_D356_T0.6770.59
115_L161_T0.6740.58
258_S263_T0.6740.58
54_V165_Y0.6740.58
113_T121_T0.6730.58
371_K378_A0.6720.58
212_T216_K0.6710.58
283_G286_I0.6700.58
175_M203_Y0.6690.58
213_K227_Q0.6650.57
229_L255_K0.6620.57
110_A171_S0.6610.57
234_D237_V0.6610.57
264_V310_K0.6600.57
55_M326_A0.6570.56
120_K445_N0.6540.56
382_V409_I0.6530.56
154_T246_L0.6520.56
71_M299_M0.6520.56
232_N237_V0.6510.56
140_E157_I0.6490.56
193_I196_L0.6490.56
251_D294_D0.6470.55
82_K85_E0.6470.55
49_I147_K0.6470.55
370_V373_V0.6460.55
241_F257_P0.6460.55
195_V279_L0.6450.55
184_D209_R0.6450.55
89_N309_Y0.6440.55
169_N177_Q0.6430.55
293_N414_S0.6420.55
85_E309_Y0.6410.55
57_G326_A0.6400.55
99_D102_D0.6390.54
64_Y298_A0.6380.54
56_I280_M0.6380.54
78_P83_L0.6350.54
179_A204_F0.6340.54
148_S272_Q0.6340.54
441_R444_I0.6330.54
281_V312_A0.6330.54
183_M211_L0.6310.53
56_I355_K0.6310.53
217_Q227_Q0.6290.53
251_D408_K0.6290.53
323_L434_G0.6280.53
179_A211_L0.6260.53
81_P86_F0.6260.53
89_N433_Y0.6260.53
147_K275_K0.6220.52
419_T424_T0.6220.52
112_G165_Y0.6220.52
110_A124_N0.6210.52
439_K456_I0.6200.52
76_D81_P0.6200.52
381_K386_E0.6190.52
107_D123_N0.6190.52
327_T428_V0.6190.52
45_D438_E0.6170.52
66_R427_E0.6170.52
170_E174_N0.6170.52
223_V228_A0.6150.52
55_M308_A0.6130.51
92_G442_G0.6130.51
93_M443_L0.6120.51
405_A409_I0.6100.51
116_A137_S0.6090.51
56_I327_T0.6080.51
221_S232_N0.6060.51
118_G195_V0.6040.50
382_V389_S0.6030.50
355_K359_F0.6020.50
164_A280_M0.6000.50
370_V375_A0.5970.50
371_K375_A0.5970.50
114_A120_K0.5950.49
258_S261_D0.5950.49
306_E431_Y0.5930.49
384_S387_I0.5920.49
204_F240_L0.5920.49
56_I165_Y0.5900.49
232_N236_Q0.5900.49
359_F362_K0.5890.49
258_S289_A0.5870.48
375_A382_V0.5860.48
71_M296_V0.5860.48
225_T255_K0.5860.48
113_T301_E0.5860.48
180_N214_E0.5840.48
75_G300_S0.5830.48
113_T450_A0.5820.48
232_N269_R0.5810.48
361_A364_I0.5810.48
197_L269_R0.5800.48
113_T127_G0.5790.47
111_A115_L0.5790.47
78_P82_K0.5770.47
371_K412_T0.5760.47
368_K372_D0.5760.47
206_R209_R0.5760.47
277_F316_A0.5750.47
313_I321_H0.5750.47
159_H285_Q0.5710.47
232_N235_Q0.5700.46
290_A325_I0.5690.46
374_L378_A0.5680.46
382_V387_I0.5660.46
283_G309_Y0.5660.46
192_K236_Q0.5660.46
112_G450_A0.5650.46
183_M238_L0.5650.46
246_L255_K0.5650.46
400_K403_S0.5640.46
66_R69_R0.5630.46
325_I430_V0.5610.45
297_G409_I0.5600.45
74_N77_T0.5590.45
327_T430_V0.5590.45
165_Y280_M0.5590.45
153_A157_I0.5580.45
335_F338_G0.5580.45
176_D202_S0.5580.45
115_L325_I0.5580.45
56_I305_F0.5570.45
121_T335_F0.5570.45
157_I179_A0.5560.45
54_V453_I0.5550.45
400_K410_F0.5540.45
195_V269_R0.5530.44
227_Q231_K0.5520.44
62_T65_I0.5510.44
191_H236_Q0.5500.44
323_L456_I0.5490.44
67_A335_F0.5490.44
206_R211_L0.5480.44
232_N238_L0.5460.44
64_Y67_A0.5440.43
385_E388_K0.5430.43
228_A401_G0.5430.43
121_T124_N0.5420.43
227_Q391_E0.5410.43
110_A121_T0.5410.43
105_I425_G0.5410.43
210_N214_E0.5400.43
330_H423_H0.5400.43
91_T441_R0.5390.43
291_H330_H0.5390.43
221_S236_Q0.5380.43
56_I116_A0.5380.43
372_D375_A0.5370.43
381_K384_S0.5360.43
152_V279_L0.5350.42
385_E389_S0.5350.42
163_A178_I0.5310.42
353_A356_T0.5300.42
323_L440_F0.5300.42
277_F417_G0.5290.42
95_M427_E0.5280.42
105_I413_R0.5270.41
160_A172_R0.5270.41
56_I112_G0.5260.41
160_A330_H0.5250.41
438_E455_K0.5250.41
328_A428_V0.5250.41
229_L241_F0.5240.41
362_K365_S0.5240.41
229_L242_A0.5230.41
206_R214_E0.5220.41
313_I317_K0.5220.41
268_D274_K0.5220.41
138_V454_F0.5210.41
434_G440_F0.5200.41
296_V353_A0.5200.41
233_K314_E0.5200.41
63_P337_I0.5190.41
73_N77_T0.5170.40
267_I316_A0.5170.40
139_L166_G0.5170.40
141_E145_Q0.5170.40
157_I240_L0.5160.40
156_E202_S0.5160.40
129_D132_G0.5150.40
294_D409_I0.5150.40
373_V377_Y0.5140.40
231_K234_D0.5130.40
51_N69_R0.5120.40
53_I239_G0.5110.40
210_N403_S0.5100.40
337_I425_G0.5100.40
258_S304_D0.5100.40
351_L354_K0.5090.39
291_H423_H0.5090.39
106_T114_A0.5080.39
72_K76_D0.5080.39
51_N195_V0.5070.39
183_M218_A0.5060.39
142_A145_Q0.5050.39
160_A423_H0.5050.39
218_A265_S0.5040.39
440_F449_Q0.5040.39
149_T193_I0.5040.39
439_K452_I0.5030.39
127_G166_G0.5030.39
80_N85_E0.5020.39
381_K389_S0.5020.39
42_K325_I0.5020.39
225_T256_T0.5010.39
79_N82_K0.5000.38
136_K140_E0.5000.38
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3e2dA 2 0.8764 100 0.148 Contact Map
1k7hA 2 0.9002 100 0.276 Contact Map
2x98A 4 0.8655 100 0.325 Contact Map
1zedA 2 0.9046 100 0.363 Contact Map
3wbhA 2 0.8742 100 0.365 Contact Map
3a52A 2 0.8633 100 0.379 Contact Map
3tg0A 2 0.833 100 0.514 Contact Map
2w5vA 2 0.7419 100 0.671 Contact Map
2mlyB 1 0.2798 100 0.79 Contact Map
3q3qA 2 0.8482 99.9 0.839 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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