GREMLIN Database
PPB3 - Alkaline phosphatase 3
UniProt: P19405 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 462 (407)
Sequences: 1915 (1314)
Seq/√Len: 65.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
202_R207_E5.2341.00
218_D221_D4.2511.00
203_N206_K4.1691.00
240_P249_I3.9801.00
303_K307_D3.5221.00
141_A266_D3.3941.00
219_R223_L3.3251.00
257_N304_A3.2661.00
219_R254_D3.2291.00
278_Q281_W3.1981.00
129_K137_E3.0511.00
151_T168_M2.9131.00
91_P103_A2.7671.00
136_K187_D2.7471.00
99_T104_A2.7351.00
149_E168_M2.7321.00
132_L271_F2.6361.00
150_I189_L2.5591.00
148_S277_S2.5411.00
205_I215_Y2.4961.00
258_T261_K2.4691.00
159_G175_Y2.4481.00
258_T262_K2.4331.00
146_A277_S2.4051.00
253_K297_D2.4031.00
253_K304_A2.3851.00
298_F302_Y2.3801.00
87_Q426_V2.3761.00
154_T273_M2.3541.00
190_L232_G2.3181.00
253_K300_Q2.3061.00
276_G298_F2.3061.00
190_L230_V2.2981.00
301_A305_A2.2731.00
79_F299_E2.2721.00
51_G279_I2.2501.00
242_K297_D2.1441.00
194_K215_Y2.0791.00
105_A157_S2.0431.00
62_Y296_E2.0421.00
57_S422_E2.0081.00
130_T133_E1.9521.00
78_A303_K1.9311.00
245_R251_S1.9001.00
297_D300_Q1.8981.00
242_K251_S1.8821.00
132_L142_T1.8741.00
55_G324_S1.8741.00
59_T427_Y1.8721.00
169_N196_N1.8371.00
118_A121_V1.7911.00
261_K308_F1.7751.00
78_A299_E1.7741.00
50_I108_M1.7671.00
62_Y295_M1.7441.00
318_V436_F1.7170.99
85_G440_I1.7100.99
114_T118_A1.6960.99
260_I305_A1.6790.99
85_G426_V1.6680.99
49_L305_A1.6670.99
55_G321_A1.6670.99
162_D174_D1.6610.99
304_A307_D1.6460.99
256_T305_A1.6450.99
119_I156_A1.6310.99
110_A120_A1.6280.99
131_V449_V1.6190.99
244_D290_G1.6090.99
120_A157_S1.5960.99
49_L298_F1.5960.99
259_A272_L1.5870.99
134_A446_A1.5770.99
115_Y118_A1.5590.99
91_P116_N1.5530.99
287_D290_G1.5320.99
87_Q445_I1.5310.99
140_K453_L1.5150.99
47_I305_A1.5130.99
253_K301_A1.4830.98
133_E161_H1.4730.98
46_V453_L1.4730.98
241_K278_Q1.4670.98
256_T274_V1.4650.98
144_L158_F1.4610.98
131_V446_A1.4580.98
92_D115_Y1.4570.98
242_K245_R1.4560.98
324_S422_E1.4520.98
309_A317_V1.4520.98
58_Y283_G1.4520.98
43_I452_A1.4210.98
444_E447_K1.4170.98
168_M238_G1.4120.98
294_E297_D1.4110.98
230_V262_K1.4080.98
110_A128_A1.4070.98
56_V425_P1.4010.98
149_E238_G1.3920.98
135_A453_L1.3870.98
99_T103_A1.3810.98
195_S198_D1.3810.98
149_E237_G1.3790.97
206_K209_K1.3780.97
87_Q440_I1.3750.97
310_K313_K1.3750.97
176_F208_F1.3720.97
426_V440_I1.3710.97
283_G291_A1.3700.97
120_A130_T1.3520.97
152_H238_G1.3520.97
257_N261_K1.3500.97
195_S237_G1.3480.97
49_L276_G1.3450.97
105_A109_S1.3300.97
62_Y77_T1.3290.97
49_L317_V1.3190.97
190_L234_F1.3170.97
213_Y229_Q1.3100.97
144_L159_G1.3070.97
148_S281_W1.3060.97
63_R423_D1.3060.97
85_G437_A1.3050.96
92_D123_N1.3030.96
309_A429_Y1.3030.96
435_T451_K1.3000.96
235_A249_I1.2850.96
309_A315_T1.2850.96
50_I154_T1.2820.96
91_P99_T1.2820.96
55_G422_E1.2820.96
436_F448_N1.2800.96
96_Q116_N1.2770.96
63_R80_D1.2760.96
88_T441_D1.2750.96
109_S158_F1.2720.96
143_G272_L1.2710.96
241_K285_D1.2520.96
318_V449_V1.2510.96
306_I429_Y1.2350.95
66_K299_E1.2340.95
426_V445_I1.2320.95
336_Y343_I1.2030.94
152_H281_W1.2030.94
120_A128_A1.2000.94
301_A304_A1.1990.94
108_M445_I1.1950.94
305_A309_A1.1900.94
187_D229_Q1.1890.94
88_T439_Q1.1840.94
54_M59_T1.1760.94
232_G255_M1.1690.94
119_I162_D1.1660.93
298_F319_A1.1620.93
276_G301_A1.1570.93
238_G281_W1.1510.93
66_K296_E1.1460.93
228_S265_K1.1420.93
238_G278_Q1.1390.93
329_S332_A1.1350.93
382_E389_A1.1320.92
99_T442_N1.1310.92
149_E152_H1.1310.92
90_Y441_D1.1300.92
382_E385_S1.1270.92
48_V271_F1.1260.92
279_I295_M1.1220.92
235_A240_P1.1170.92
132_L186_I1.1090.92
151_T197_F1.1080.92
208_F231_L1.1080.92
51_G320_T1.1050.92
47_I309_A1.1020.91
73_V76_P1.0980.91
177_D202_R1.0970.91
332_A336_Y1.0860.91
136_K142_T1.0860.91
151_T238_G1.0800.91
132_L158_F1.0800.91
109_S120_A1.0760.90
217_D221_D1.0750.90
444_E448_N1.0730.90
188_V259_A1.0720.90
99_T116_N1.0620.90
427_Y439_Q1.0610.90
377_A381_A1.0520.89
152_H168_M1.0500.89
82_Y429_Y1.0490.89
223_L258_T1.0490.89
103_A116_N1.0480.89
122_D126_S1.0470.89
252_L297_D1.0460.89
377_A382_E1.0420.89
145_V259_A1.0420.89
276_G305_A1.0390.89
385_S388_E1.0350.89
340_S343_I1.0330.89
336_Y339_F1.0310.88
277_S281_W1.0300.88
135_A455_Y1.0260.88
243_I349_T1.0240.88
222_M258_T1.0240.88
234_F255_M1.0170.88
194_K217_D1.0150.88
306_I317_V1.0120.87
239_L249_I1.0110.87
83_L433_S1.0060.87
190_L216_V1.0050.87
206_K210_K0.9980.87
49_L302_Y0.9910.86
446_A450_F0.9890.86
108_M273_M0.9820.86
239_L255_M0.9760.86
82_Y306_I0.9760.86
215_Y233_L0.9750.85
115_Y123_N0.9660.85
46_V449_V0.9650.85
329_S336_Y0.9650.85
324_S420_T0.9600.85
304_A308_F0.9580.84
134_A450_F0.9460.84
440_I444_E0.9430.83
60_S64_Y0.9400.83
78_A306_I0.9390.83
227_D262_K0.9360.83
241_K282_A0.9350.83
135_A142_T0.9310.83
145_V188_V0.9300.83
160_S171_I0.9190.82
243_I289_V0.9160.82
128_A161_H0.9160.82
195_S236_D0.9160.82
336_Y340_S0.9120.81
297_D301_A0.9110.81
86_Q425_P0.9090.81
309_A313_K0.9090.81
256_T260_I0.9030.81
151_T196_N0.9010.81
190_L222_M0.9010.81
188_V230_V0.9000.81
141_A268_D0.9000.81
260_I308_F0.9000.81
78_A81_Q0.8980.80
69_K293_S0.8970.80
253_K257_N0.8940.80
243_I290_G0.8930.80
129_K134_A0.8900.80
151_T172_A0.8890.80
67_D75_E0.8890.80
286_N326_G0.8870.80
104_A442_N0.8860.79
133_E137_E0.8850.79
48_V318_V0.8840.79
382_E409_R0.8790.79
305_A317_V0.8790.79
167_N170_S0.8770.79
144_L271_F0.8760.79
168_M237_G0.8750.79
51_G54_M0.8720.78
205_I217_D0.8720.78
160_S175_Y0.8720.78
98_V331_G0.8700.78
241_K281_W0.8690.78
54_M295_M0.8640.78
65_L296_E0.8630.78
130_T161_H0.8560.77
83_L437_A0.8550.77
311_K314_H0.8550.77
141_A187_D0.8530.77
317_V429_Y0.8470.76
310_K314_H0.8430.76
54_M279_I0.8420.76
363_V366_T0.8410.76
296_E299_E0.8400.76
274_V301_A0.8370.75
328_Y336_Y0.8330.75
244_D294_E0.8310.75
59_T425_P0.8300.75
136_K185_K0.8290.75
222_M232_G0.8290.75
194_K236_D0.8280.75
152_H166_K0.8270.75
282_A290_G0.8250.74
48_V320_T0.8200.74
117_N164_S0.8180.74
379_T382_E0.8180.74
140_K269_G0.8170.74
106_T114_T0.8160.74
310_K429_Y0.8140.73
383_I386_V0.8130.73
223_L254_D0.8120.73
175_Y208_F0.8100.73
173_D204_L0.8090.73
366_T370_Y0.8040.73
79_F427_Y0.8010.72
377_A385_S0.8010.72
143_G188_V0.8000.72
190_L255_M0.7990.72
199_R203_N0.7980.72
403_E407_N0.7950.72
84_V428_A0.7920.71
289_V404_N0.7890.71
326_G414_W0.7860.71
55_G328_Y0.7840.71
282_A287_D0.7830.71
216_V230_V0.7810.70
136_K184_H0.7800.70
49_L140_K0.7780.70
384_Q445_I0.7780.70
143_G269_G0.7770.70
49_L274_V0.7760.70
102_A119_I0.7730.70
55_G91_P0.7710.69
61_A292_M0.7710.69
54_M320_T0.7690.69
227_D230_V0.7690.69
56_V86_Q0.7660.69
188_V228_S0.7640.69
316_L436_F0.7630.69
299_E303_K0.7630.69
109_S273_M0.7620.68
141_A265_K0.7580.68
383_I387_E0.7570.68
384_Q388_E0.7560.68
48_V108_M0.7560.68
77_T299_E0.7560.68
289_V293_S0.7560.68
276_G279_I0.7550.68
300_Q303_K0.7540.68
159_G176_F0.7530.67
264_N308_F0.7510.67
198_D201_D0.7490.67
105_A158_F0.7490.67
113_K441_D0.7490.67
430_G433_S0.7490.67
51_G321_A0.7480.67
68_N71_T0.7470.67
296_E300_Q0.7450.67
302_Y319_A0.7450.67
205_I231_L0.7440.67
119_I157_S0.7440.67
385_S389_A0.7430.66
149_E278_Q0.7400.66
341_E367_L0.7400.66
139_G268_D0.7390.66
215_Y254_D0.7360.66
346_A349_T0.7360.66
144_L186_I0.7360.66
316_L433_S0.7340.66
285_D416_T0.7330.65
372_D417_G0.7320.65
135_A140_K0.7310.65
377_A380_K0.7290.65
173_D177_D0.7290.65
93_D115_Y0.7260.65
216_V222_M0.7260.65
87_Q318_V0.7230.64
162_D167_N0.7220.64
222_M248_D0.7200.64
65_L293_S0.7200.64
204_L207_E0.7200.64
350_P354_A0.7190.64
170_S173_D0.7190.64
44_K314_H0.7180.64
267_K313_K0.7180.64
440_I448_N0.7130.63
307_D310_K0.7130.63
58_Y324_S0.7110.63
162_D170_S0.7110.63
89_T424_V0.7080.63
112_I128_A0.7070.63
341_E344_K0.7070.63
386_V389_A0.7040.62
108_M154_T0.7020.62
445_I449_V0.7010.62
209_K215_Y0.7000.62
428_A436_F0.6990.62
50_I273_M0.6940.61
51_G319_A0.6910.61
43_I140_K0.6900.61
318_V446_A0.6900.61
320_T426_V0.6880.61
108_M446_A0.6870.60
48_V158_F0.6870.60
127_E161_H0.6870.60
104_A108_M0.6870.60
320_T424_V0.6850.60
93_D96_Q0.6850.60
222_M249_I0.6850.60
372_D418_G0.6840.60
337_N344_K0.6840.60
220_K223_L0.6830.60
251_S282_A0.6810.60
65_L289_V0.6790.59
207_E210_K0.6780.59
383_I388_E0.6780.59
340_S345_A0.6740.59
209_K212_G0.6740.59
56_V422_E0.6720.59
244_D287_D0.6720.59
359_D362_D0.6720.59
107_A113_K0.6710.59
147_T255_M0.6690.58
257_N308_F0.6660.58
365_K369_T0.6660.58
437_A440_I0.6660.58
173_D202_R0.6650.58
50_I158_F0.6590.57
49_L319_A0.6580.57
190_L272_L0.6570.57
77_T296_E0.6550.57
147_T239_L0.6540.57
339_F344_K0.6530.57
366_T372_D0.6510.56
220_K248_D0.6500.56
216_V221_D0.6500.56
176_F207_E0.6490.56
60_S423_D0.6490.56
311_K429_Y0.6490.56
50_I298_F0.6490.56
203_N207_E0.6480.56
65_L292_M0.6470.56
49_L301_A0.6470.56
339_F342_P0.6460.56
270_F308_F0.6460.56
47_I232_G0.6440.55
98_V116_N0.6440.55
367_L370_Y0.6440.55
220_K224_K0.6420.55
369_T372_D0.6420.55
50_I320_T0.6390.55
66_K70_K0.6370.55
169_N195_S0.6360.55
188_V272_L0.6360.55
286_N410_S0.6350.54
99_T107_A0.6330.54
58_Y291_A0.6320.54
225_N228_S0.6300.54
65_L69_K0.6300.54
348_R352_F0.6290.54
283_G370_Y0.6290.54
174_D178_E0.6240.53
206_K220_K0.6220.53
152_H278_Q0.6210.53
147_T278_Q0.6210.53
251_S297_D0.6210.53
234_F250_P0.6210.53
222_M234_F0.6200.53
170_S200_K0.6190.53
225_N229_Q0.6170.52
197_F233_L0.6170.52
307_D311_K0.6170.52
367_L371_I0.6150.52
168_M196_N0.6140.52
318_V337_N0.6130.52
218_D248_D0.6120.52
163_H167_N0.6110.52
106_T446_A0.6100.52
316_L430_G0.6090.51
140_K268_D0.6090.51
352_F356_K0.6090.51
186_I189_L0.6080.51
366_T371_I0.6080.51
205_I209_K0.6080.51
337_N340_S0.6080.51
103_A117_N0.6040.51
39_N434_E0.6030.51
225_N230_V0.6030.51
106_T120_A0.6030.51
415_T420_T0.6020.51
339_F343_I0.6020.51
382_E386_V0.6020.51
45_N188_V0.5990.50
68_N75_E0.5980.50
86_Q439_Q0.5980.50
251_S256_T0.5970.50
284_H323_H0.5960.50
211_A258_T0.5950.50
188_V262_K0.5950.50
157_S273_M0.5950.50
342_P346_A0.5950.50
109_S271_F0.5940.50
103_A164_S0.5940.50
45_N63_R0.5930.50
82_Y302_Y0.5930.50
368_K371_I0.5910.49
428_A433_S0.5900.49
185_K229_Q0.5900.49
332_A337_N0.5890.49
109_S130_T0.5880.49
377_A384_Q0.5880.49
274_V305_A0.5870.49
60_S80_D0.5870.49
323_H419_H0.5860.49
378_L382_E0.5850.49
190_L262_K0.5820.48
407_N414_W0.5810.48
176_F204_L0.5800.48
330_I336_Y0.5800.48
306_I314_H0.5790.48
105_A446_A0.5790.48
284_H419_H0.5770.48
153_A323_H0.5730.47
111_G188_V0.5730.47
257_N303_K0.5730.47
153_A419_H0.5720.47
67_D71_T0.5710.47
146_A150_I0.5710.47
205_I233_L0.5690.47
78_A302_Y0.5670.47
340_S344_K0.5660.46
112_I122_D0.5660.46
358_A362_D0.5660.46
47_I190_L0.5650.46
341_E345_A0.5630.46
100_D116_N0.5610.46
188_V232_G0.5600.46
260_I309_A0.5590.46
153_A284_H0.5590.46
306_I310_K0.5570.45
77_T295_M0.5570.45
214_S225_N0.5560.45
62_Y292_M0.5540.45
208_F324_S0.5530.45
199_R202_R0.5530.45
328_Y332_A0.5520.45
199_R207_E0.5520.45
270_F309_A0.5520.45
361_A364_E0.5520.45
332_A339_F0.5520.45
376_L381_A0.5510.45
71_T75_E0.5500.45
60_S63_R0.5500.45
332_A340_S0.5500.45
401_A405_I0.5500.45
152_H277_S0.5490.44
225_N231_L0.5490.44
132_L159_G0.5480.44
166_K238_G0.5480.44
229_Q265_K0.5480.44
107_A191_G0.5470.44
84_V437_A0.5470.44
80_D423_D0.5460.44
380_K389_A0.5440.44
85_G438_G0.5440.44
404_N408_K0.5440.44
39_N42_E0.5410.43
363_V367_L0.5410.43
251_S294_E0.5400.43
380_K384_Q0.5400.43
56_V59_T0.5390.43
152_H241_K0.5360.43
364_E367_L0.5360.43
435_T448_N0.5350.43
328_Y331_G0.5350.43
134_A455_Y0.5340.43
120_A159_G0.5340.43
159_G189_L0.5330.43
88_T423_D0.5310.42
370_Y373_Q0.5310.42
158_F273_M0.5300.42
82_Y317_V0.5290.42
155_P284_H0.5290.42
184_H229_Q0.5290.42
380_K385_S0.5280.42
210_K220_K0.5280.42
79_F302_Y0.5280.42
368_K372_D0.5260.42
362_D387_E0.5250.42
106_T294_E0.5240.42
299_E427_Y0.5230.41
316_L452_A0.5220.41
84_V440_I0.5220.41
168_M197_F0.5220.41
50_I109_S0.5210.41
131_V135_A0.5210.41
48_V449_V0.5190.41
72_K268_D0.5180.41
145_V272_L0.5180.41
241_K277_S0.5170.41
142_T186_I0.5160.41
103_A114_T0.5150.41
399_D403_E0.5150.41
60_S330_I0.5140.40
35_K38_G0.5130.40
276_G302_Y0.5120.40
223_L265_K0.5100.40
362_D366_T0.5100.40
155_P323_H0.5100.40
369_T373_Q0.5090.40
261_K264_N0.5090.40
143_G263_L0.5080.40
154_T157_S0.5080.40
375_K382_E0.5080.40
47_I315_T0.5070.40
434_E451_K0.5070.40
143_G260_I0.5060.40
327_G330_I0.5060.40
56_V60_S0.5050.39
397_D400_N0.5040.39
153_A165_R0.5040.39
337_N342_P0.5020.39
176_F211_A0.5020.39
287_D405_I0.5010.39
80_D291_A0.5010.39
290_G405_I0.5010.39
384_Q387_E0.5010.39
274_V309_A0.5000.39
368_K373_Q0.5000.39
436_F445_I0.5000.39
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3e2dA 2 0.8766 100 0.175 Contact Map
1k7hA 2 0.9134 100 0.283 Contact Map
2x98A 4 0.8658 100 0.341 Contact Map
1zedA 2 0.9134 100 0.367 Contact Map
3wbhA 2 0.8745 100 0.385 Contact Map
3a52A 2 0.8615 100 0.389 Contact Map
3tg0A 2 0.8333 100 0.523 Contact Map
2w5vA 2 0.7381 100 0.68 Contact Map
2mlyB 1 0.2684 100 0.806 Contact Map
3q3qA 2 0.8506 99.9 0.844 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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