GREMLIN Database
HEM3 - Porphobilinogen deaminase
UniProt: P16616 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 314 (297)
Sequences: 3530 (2292)
Seq/√Len: 133.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
220_T225_K4.3371.00
156_Q160_T4.3081.00
6_K38_K3.2261.00
8_G74_I3.2051.00
101_E174_S2.9081.00
99_E221_D2.8821.00
23_I27_K2.8801.00
11_R39_E2.7031.00
170_A186_F2.7021.00
237_A291_A2.6071.00
200_A228_V2.5721.00
266_S270_K2.4251.00
159_E182_V2.3961.00
123_V145_K2.3751.00
206_R209_D2.3241.00
275_E289_C2.3131.00
11_R41_V2.2781.00
25_K28_E2.2711.00
257_E278_T2.2701.00
277_V292_L2.2121.00
132_S136_L2.1241.00
93_V207_E2.1231.00
91_G207_E2.0781.00
275_E292_L2.0641.00
145_K163_Y2.0171.00
114_K117_E1.9951.00
47_I50_V1.9881.00
4_T34_A1.9661.00
151_I176_M1.9611.00
123_V143_T1.9451.00
7_V23_I1.9361.00
13_K152_D1.9261.00
221_D224_T1.9161.00
4_T36_E1.9031.00
147_I166_I1.9021.00
259_E278_T1.8981.00
93_V213_L1.8901.00
95_G205_C1.8881.00
261_T276_T1.8841.00
279_G285_V1.8691.00
264_V298_A1.8521.00
67_Q71_N1.8341.00
98_P201_L1.7921.00
20_K24_Q1.7901.00
234_F262_G1.7691.00
66_E204_E1.7671.00
225_K229_L1.7621.00
259_E276_T1.7441.00
21_W25_K1.7341.00
237_A287_K1.7081.00
277_V289_C1.6951.00
81_M197_G1.6951.00
291_A295_D1.6791.00
19_T37_I1.6571.00
29_I218_Q1.6341.00
210_E214_A1.6111.00
275_E296_K1.5951.00
96_C220_T1.5891.00
188_E191_R1.5781.00
74_I77_A1.5711.00
104_R186_F1.5661.00
70_L92_L1.5631.00
16_M39_E1.5431.00
41_V46_R1.5021.00
247_A263_L1.4981.00
138_E191_R1.4951.00
20_K23_I1.4821.00
152_D178_W1.4821.00
196_V248_G1.4781.00
229_L283_E1.4631.00
205_C213_L1.4611.00
271_I300_D1.4581.00
209_D212_L1.4521.00
281_D284_E1.4521.00
97_I200_A1.4331.00
30_N218_Q1.4251.00
136_L144_I1.4251.00
9_S37_I1.4241.00
25_K29_I1.4231.00
27_K34_A1.4051.00
227_T252_L1.4031.00
113_V117_E1.4021.00
68_A73_E1.3961.00
95_G213_L1.3911.00
104_R170_A1.3841.00
6_K36_E1.3721.00
277_V288_R1.3481.00
222_E226_R1.3431.00
23_I35_F1.3391.00
288_R292_L1.3201.00
185_E191_R1.3141.00
124_I165_A1.3141.00
116_S191_R1.3041.00
284_E288_R1.2991.00
102_D170_A1.2941.00
81_M202_A1.2931.00
116_S185_E1.2931.00
230_A260_M1.2911.00
292_L296_K1.2821.00
187_L192_C1.2721.00
234_F289_C1.2711.00
287_K291_A1.2671.00
174_S179_K1.2601.00
24_Q28_E1.2531.00
251_V261_T1.2491.00
106_A170_A1.2401.00
7_V76_M1.2361.00
132_S146_W1.2341.00
251_V259_E1.2311.00
17_T21_W1.2191.00
38_K73_E1.2031.00
5_I212_L1.2011.00
163_Y166_I1.1900.99
170_A174_S1.1850.99
286_G290_A1.1830.99
19_T23_I1.1790.99
132_S144_I1.1760.99
261_T274_K1.1680.99
81_M228_V1.1620.99
40_I65_I1.1510.99
212_L216_F1.1510.99
237_A294_A1.1440.99
109_S184_T1.1410.99
108_I155_L1.1380.99
265_A272_I1.1280.99
293_M298_A1.1270.99
214_A217_S1.1260.99
133_A265_A1.1180.99
124_I135_L1.1170.99
294_A299_K1.1170.99
157_K162_D1.1150.99
114_K185_E1.1040.99
283_E287_K1.1020.99
94_I202_A1.1000.99
40_I68_A1.0990.99
27_K35_F1.0930.99
234_F260_M1.0910.99
234_F293_M1.0890.99
137_I272_I1.0810.99
108_I173_L1.0800.99
24_Q27_K1.0740.99
183_V186_F1.0710.99
253_N278_T1.0610.99
236_N283_E1.0600.99
253_N259_E1.0550.99
88_L204_E1.0490.99
279_G282_P1.0490.99
16_M20_K1.0440.99
158_L166_I1.0410.99
22_V98_P1.0270.98
48_V152_D1.0230.98
205_C216_F1.0150.98
260_M289_C1.0070.98
11_R40_I0.9990.98
118_M122_A0.9980.98
102_D186_F0.9880.98
139_R142_L0.9880.98
178_W182_V0.9860.98
97_I228_V0.9740.98
51_T146_W0.9650.98
230_A258_I0.9630.98
32_S35_F0.9610.98
68_A74_I0.9580.98
179_K183_V0.9500.97
151_I168_L0.9470.97
20_K37_I0.9470.97
170_A183_V0.9460.97
233_A237_A0.9460.97
145_K162_D0.9460.97
124_I142_L0.9430.97
66_E92_L0.9420.97
193_L247_A0.9420.97
6_K72_E0.9370.97
223_Y227_T0.9360.97
135_L142_L0.9290.97
7_V19_T0.9290.97
113_V184_T0.9230.97
284_E287_K0.9180.97
103_P249_Y0.9180.97
7_V35_F0.9130.97
123_V163_Y0.9020.96
152_D156_Q0.8950.96
92_L204_E0.8920.96
151_I178_W0.8910.96
121_G142_L0.8910.96
240_G246_I0.8890.96
43_K47_I0.8820.96
84_M202_A0.8820.96
174_S186_F0.8750.96
262_G289_C0.8700.96
282_P286_G0.8650.95
102_D174_S0.8630.95
75_D206_R0.8630.95
210_E213_L0.8560.95
238_M262_G0.8550.95
248_G260_M0.8540.95
273_F301_L0.8500.95
122_A139_R0.8470.95
193_L261_T0.8420.95
116_S138_E0.8380.95
120_K143_T0.8380.95
168_L172_G0.8270.94
93_V205_C0.8250.94
119_K122_A0.8240.94
133_A269_G0.8240.94
122_A142_L0.8230.94
50_V54_K0.8200.94
213_L217_S0.8200.94
226_R282_P0.8170.94
58_K64_E0.8160.94
277_V285_V0.8110.94
109_S112_R0.8110.94
187_L191_R0.8110.94
81_M94_I0.8080.94
55_V60_L0.8070.93
38_K74_I0.8040.93
263_L274_K0.8030.93
107_L112_R0.8020.93
292_L295_D0.8020.93
200_A224_T0.8020.93
26_L33_F0.7960.93
43_K55_V0.7930.93
5_I35_F0.7930.93
266_S271_I0.7920.93
135_L144_I0.7890.93
120_K141_D0.7810.92
23_I37_I0.7800.92
256_D280_N0.7780.92
110_K159_E0.7730.92
52_L57_G0.7730.92
90_E207_E0.7730.92
9_S15_A0.7720.92
288_R291_A0.7720.92
5_I76_M0.7720.92
224_T228_V0.7720.92
89_P92_L0.7710.92
223_Y252_L0.7710.92
62_V92_L0.7680.92
238_M246_I0.7660.92
14_L151_I0.7640.91
104_R188_E0.7640.91
189_P249_Y0.7640.91
137_I274_K0.7580.91
271_I296_K0.7570.91
107_L165_A0.7560.91
265_A274_K0.7500.91
25_K99_E0.7490.91
21_W201_L0.7490.91
227_T250_S0.7460.91
273_F293_M0.7440.90
52_L60_L0.7440.90
263_L272_I0.7390.90
136_L143_T0.7390.90
13_K17_T0.7380.90
104_R189_P0.7370.90
11_R16_M0.7360.90
147_I163_Y0.7340.90
62_V86_A0.7330.90
52_L56_G0.7320.90
43_K46_R0.7250.89
125_G163_Y0.7250.89
81_M97_I0.7250.89
106_A173_L0.7240.89
107_L110_K0.7220.89
234_F290_A0.7180.89
56_G60_L0.7160.89
134_Q193_L0.7150.89
97_I225_K0.7140.89
115_L185_E0.7120.88
43_K64_E0.7120.88
134_Q192_C0.7070.88
16_M37_I0.7060.88
122_A141_D0.7050.88
21_W98_P0.7050.88
233_A283_E0.7020.88
8_G38_K0.7020.88
190_E261_T0.6920.87
107_L184_T0.6890.87
107_L115_L0.6880.87
215_L218_Q0.6860.86
233_A286_G0.6840.86
26_L35_F0.6830.86
193_L249_Y0.6830.86
194_P249_Y0.6830.86
17_T201_L0.6820.86
217_S220_T0.6820.86
66_E88_L0.6810.86
226_R252_L0.6790.86
234_F248_G0.6760.86
106_A183_V0.6760.86
66_E84_M0.6720.85
69_L72_E0.6710.85
227_T251_V0.6690.85
14_L101_E0.6680.85
158_L163_Y0.6670.85
208_S213_L0.6670.85
96_C216_F0.6630.85
20_K39_E0.6620.84
17_T20_K0.6610.84
86_A133_A0.6610.84
253_N257_E0.6590.84
26_L215_L0.6580.84
196_V235_L0.6570.84
30_N215_L0.6560.84
266_S269_G0.6550.84
100_R227_T0.6550.84
21_W24_Q0.6520.84
119_K139_R0.6520.84
63_K66_E0.6520.84
151_I172_G0.6510.83
138_E187_L0.6510.83
230_A282_P0.6500.83
96_C205_C0.6500.83
156_Q159_E0.6500.83
9_S39_E0.6490.83
66_E69_L0.6480.83
86_A241_G0.6460.83
115_L118_M0.6450.83
282_P285_V0.6450.83
233_A287_K0.6440.83
193_L263_L0.6440.83
275_E293_M0.6430.83
6_K75_D0.6400.82
70_L91_G0.6400.82
72_E208_S0.6340.82
22_V96_C0.6340.82
76_M216_F0.6330.82
86_A235_L0.6320.82
60_L240_G0.6290.81
22_V203_I0.6280.81
19_T35_F0.6270.81
223_Y226_R0.6250.81
241_G244_V0.6240.81
283_E286_G0.6220.81
33_F215_L0.6220.81
92_L206_R0.6190.80
55_V62_V0.6190.80
19_T76_M0.6170.80
121_G139_R0.6170.80
211_E214_A0.6170.80
63_K70_L0.6170.80
22_V219_F0.6130.80
155_L159_E0.6090.79
42_T64_E0.6030.79
239_E299_K0.6000.78
48_V59_G0.6000.78
297_G301_L0.5980.78
51_T54_K0.5970.78
88_L92_L0.5970.78
197_G200_A0.5960.78
40_I152_D0.5960.78
135_L167_I0.5930.77
76_M205_C0.5900.77
49_D55_V0.5890.77
11_R42_T0.5890.77
65_I98_P0.5880.77
13_K176_M0.5860.77
244_V297_G0.5860.77
47_I55_V0.5850.76
158_L165_A0.5840.76
112_R184_T0.5830.76
50_V53_S0.5820.76
253_N256_D0.5810.76
101_E171_A0.5800.76
273_F297_G0.5760.75
289_C292_L0.5750.75
238_M264_V0.5740.75
258_I285_V0.5740.75
155_L158_L0.5720.75
273_F296_K0.5700.75
101_E169_A0.5700.75
63_K68_A0.5700.75
58_K244_V0.5690.74
155_L173_L0.5680.74
254_G257_E0.5650.74
110_K113_V0.5630.74
152_D176_M0.5610.73
53_S243_Q0.5600.73
126_T132_S0.5600.73
226_R258_I0.5560.73
239_E246_I0.5560.73
171_A201_L0.5560.73
167_I192_C0.5550.73
138_E141_D0.5550.73
58_K66_E0.5550.73
194_P250_S0.5540.73
106_A186_F0.5530.72
56_G59_G0.5530.72
54_K243_Q0.5510.72
111_N164_D0.5510.72
233_A289_C0.5510.72
264_V293_M0.5500.72
46_R50_V0.5500.72
239_E262_G0.5490.72
230_A286_G0.5480.72
10_R14_L0.5470.72
127_S146_W0.5450.71
35_F76_M0.5430.71
212_L215_L0.5420.71
250_S260_M0.5410.71
250_S258_I0.5400.71
178_W183_V0.5400.71
279_G289_C0.5390.70
118_M121_G0.5390.70
125_G161_E0.5380.70
48_V129_L0.5380.70
77_A194_P0.5370.70
40_I74_I0.5370.70
294_A298_A0.5340.70
50_V55_V0.5340.70
69_L206_R0.5330.70
17_T173_L0.5330.70
63_K67_Q0.5320.70
109_S113_V0.5320.70
47_I60_L0.5310.69
10_R15_A0.5300.69
118_M165_A0.5260.69
120_K139_R0.5250.69
207_E213_L0.5250.69
8_G40_I0.5230.68
262_G286_G0.5230.68
249_Y261_T0.5220.68
63_K89_P0.5220.68
87_V236_N0.5220.68
80_S202_A0.5170.67
248_G286_G0.5160.67
252_L258_I0.5140.67
262_G290_A0.5140.67
57_G62_V0.5130.67
134_Q238_M0.5130.67
243_Q246_I0.5110.66
80_S203_I0.5100.66
214_A218_Q0.5090.66
229_L282_P0.5090.66
134_Q263_L0.5060.66
158_L161_E0.5060.66
115_L187_L0.5050.66
108_I184_T0.5040.65
264_V297_G0.5030.65
157_K163_Y0.5030.65
226_R279_G0.5020.65
226_R280_N0.5020.65
97_I202_A0.5020.65
144_I192_C0.5020.65
43_K63_K0.5000.65
13_K20_K0.5000.65
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4mlvA 1 0.9108 100 0.024 Contact Map
1gtkA 1 0.9299 100 0.026 Contact Map
3ecrA 1 0.9204 100 0.033 Contact Map
4htgA 2 0.9331 100 0.042 Contact Map
4qhqA 1 0.6497 97 0.933 Contact Map
4gotA 1 0.6783 97 0.934 Contact Map
3ir1A 3 0.672 96.8 0.935 Contact Map
4ntlA 1 0.6656 96.8 0.936 Contact Map
3un6A 1 0.8248 96.8 0.936 Contact Map
3up9A 1 0.672 96.7 0.936 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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