GREMLIN Database
RISA - Riboflavin synthase
UniProt: P16440 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 215 (195)
Sequences: 3744 (2544)
Seq/√Len: 182.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
88_A91_G3.0721.00
106_A122_L2.5511.00
69_A134_L2.4831.00
121_D154_T2.3701.00
107_E123_K2.3301.00
104_G128_L2.3211.00
141_D172_K2.2901.00
80_K171_E2.2371.00
54_T57_Q2.1941.00
132_L147_I2.1571.00
130_K190_Y2.0881.00
52_D59_T2.0761.00
109_T123_K2.0641.00
11_T27_K2.0611.00
13_E27_K2.0501.00
87_M93_F2.0411.00
131_T190_Y2.0171.00
74_D177_K1.9921.00
121_D156_T1.9671.00
118_V164_I1.9611.00
151_T154_T1.9221.00
11_T80_K1.8661.00
27_K56_N1.8501.00
123_K153_D1.8471.00
25_T59_T1.8181.00
172_K176_S1.7641.00
89_A97_F1.7591.00
143_V169_F1.7521.00
64_P68_K1.7461.00
140_V178_V1.7281.00
162_H166_E1.7211.00
149_G156_T1.6761.00
10_G83_L1.6471.00
111_I120_Y1.6271.00
43_V81_V1.6081.00
36_H39_D1.5881.00
107_E177_K1.5851.00
114_K119_Y1.5811.00
35_V41_I1.5661.00
17_K22_M1.5601.00
124_M180_I1.5581.00
63_M158_S1.5541.00
89_A93_F1.5511.00
140_V180_I1.5431.00
111_I118_V1.5151.00
108_I120_Y1.5081.00
108_I172_K1.4951.00
75_L79_S1.4931.00
8_E28_C1.4801.00
46_I70_T1.4641.00
123_K154_T1.4471.00
25_T57_Q1.4401.00
110_R121_D1.4331.00
7_E85_R1.4001.00
8_E29_S1.3821.00
37_L162_H1.3801.00
185_I189_M1.3691.00
108_I122_L1.3491.00
117_A158_S1.3431.00
98_V139_T1.3321.00
133_V186_G1.3321.00
7_E92_R1.2981.00
143_V168_I1.2931.00
43_V58_F1.2861.00
132_L155_V1.2591.00
65_E135_K1.2581.00
120_Y164_I1.2541.00
97_F184_M1.2531.00
119_Y156_T1.2441.00
9_T80_K1.2221.00
93_F97_F1.2171.00
76_T79_S1.2161.00
141_D168_I1.2131.00
148_F156_T1.2091.00
44_N141_D1.2051.00
164_I170_S1.1961.00
132_L138_I1.1911.00
106_A140_V1.1821.00
44_N71_S1.1801.00
120_Y159_L1.1761.00
122_L157_I1.1671.00
148_F158_S1.1611.00
133_V191_R1.1591.00
124_M132_L1.1411.00
97_F185_I1.1401.00
133_V184_M1.1351.00
65_E69_A1.1151.00
51_T61_D1.1121.00
71_S177_K1.1111.00
68_K114_K1.1081.00
27_K57_Q1.1071.00
24_L62_V1.1061.00
143_V159_L1.0971.00
140_V157_I1.0951.00
44_N75_L1.0641.00
169_F172_K1.0621.00
23_A51_T1.0611.00
133_V187_K1.0601.00
109_T154_T1.0581.00
131_T182_C1.0571.00
64_P114_K1.0521.00
85_R92_R1.0511.00
143_V172_K1.0501.00
13_E57_Q1.0491.00
105_T177_K1.0461.00
32_L35_V1.0331.00
35_V50_V1.0271.00
122_L178_V1.0271.00
35_V53_F1.0261.00
190_Y194_H1.0210.99
113_E118_V1.0030.99
21_A116_N1.0010.99
15_M22_M0.9960.99
119_Y158_S0.9930.99
30_K33_E0.9710.99
80_K170_S0.9610.99
111_I173_T0.9590.99
99_S184_M0.9560.99
10_G26_I0.9560.99
108_I178_V0.9510.99
50_V53_F0.9500.99
134_L149_G0.9490.99
106_A180_I0.9450.99
14_S25_T0.9320.99
88_A93_F0.9280.99
9_T168_I0.9260.99
63_M148_F0.9250.99
34_D85_R0.9210.99
173_T176_S0.9200.99
138_I155_V0.9180.99
124_M129_T0.9130.99
39_D94_G0.9120.99
34_D92_R0.9090.99
86_A92_R0.9050.99
108_I111_I0.8970.99
71_S179_N0.8940.99
12_I81_V0.8940.99
120_Y169_F0.8900.99
110_R174_I0.8900.99
16_K23_A0.8860.99
119_Y148_F0.8850.99
159_L164_I0.8780.98
14_S77_K0.8750.98
104_G125_D0.8740.98
99_S102_V0.8710.98
168_I172_K0.8630.98
23_A59_T0.8510.98
22_M67_V0.8450.98
37_L53_F0.8420.98
8_E30_K0.8410.98
12_I26_I0.8340.98
31_I34_D0.8330.98
21_A115_S0.8310.98
34_D94_G0.8310.98
12_I75_L0.8270.98
140_V155_V0.8150.98
124_M155_V0.8090.97
18_A23_A0.8090.97
46_I102_V0.8040.97
72_L75_L0.7990.97
128_L182_C0.7980.97
87_M91_G0.7960.97
12_I15_M0.7900.97
124_M128_L0.7880.97
8_E31_I0.7870.97
9_T166_E0.7850.97
111_I174_I0.7760.97
61_D161_P0.7760.97
104_G124_M0.7730.97
143_V167_T0.7720.97
129_T191_R0.7690.96
39_D92_R0.7680.96
191_R195_K0.7570.96
28_C32_L0.7510.96
63_M117_A0.7500.96
117_A164_I0.7370.96
48_L66_T0.7350.95
28_C31_I0.7340.95
23_A61_D0.7220.95
186_G192_F0.7210.95
73_N177_K0.7180.95
126_P152_E0.7120.95
23_A52_D0.7090.94
150_L191_R0.7050.94
26_I83_L0.7010.94
129_T153_D0.6960.94
112_E121_D0.6940.94
172_K178_V0.6940.94
185_I193_L0.6940.94
155_V180_I0.6890.94
35_V85_R0.6890.94
37_L51_T0.6880.94
5_I94_G0.6880.94
67_V73_N0.6860.93
44_N168_I0.6830.93
26_I60_V0.6820.93
22_M64_P0.6810.93
112_E119_Y0.6740.93
138_I180_I0.6720.93
102_V139_T0.6670.93
130_K192_F0.6660.92
53_F57_Q0.6660.92
43_V83_L0.6650.92
189_M193_L0.6620.92
25_T54_T0.6580.92
31_I35_V0.6560.92
49_T162_H0.6560.92
20_H64_P0.6540.92
186_G189_M0.6520.92
62_V66_T0.6520.92
7_E91_G0.6470.91
3_T93_F0.6460.91
112_E156_T0.6450.91
88_A92_R0.6440.91
13_E25_T0.6430.91
133_V188_Y0.6430.91
44_N79_S0.6420.91
15_M77_K0.6390.91
131_T191_R0.6360.91
182_C187_K0.6350.91
13_E56_N0.6290.90
43_V75_L0.6210.90
106_A124_M0.6180.89
39_D86_A0.6180.89
26_I43_V0.6140.89
32_L58_F0.6120.89
13_E77_K0.6110.89
121_D151_T0.6090.89
48_L62_V0.6090.89
1_M4_G0.6030.88
71_S141_D0.6030.88
111_I164_I0.6030.88
63_M157_I0.6000.88
9_T170_S0.5980.88
36_H53_F0.5950.88
72_L79_S0.5930.87
29_S32_L0.5920.87
109_T174_I0.5920.87
39_D85_R0.5910.87
132_L180_I0.5900.87
137_S185_I0.5890.87
101_H137_S0.5890.87
35_V39_D0.5870.87
72_L81_V0.5840.87
137_S143_V0.5840.87
126_P153_D0.5800.86
18_A21_A0.5790.86
37_L50_V0.5780.86
122_L140_V0.5780.86
37_L166_E0.5750.86
69_A148_F0.5690.85
114_K117_A0.5680.85
119_Y149_G0.5680.85
122_L155_V0.5680.85
5_I31_I0.5660.85
132_L182_C0.5620.84
159_L169_F0.5610.84
64_P158_S0.5600.84
86_A99_S0.5580.84
43_V48_L0.5570.84
97_F137_S0.5540.84
188_Y192_F0.5540.84
159_L163_T0.5500.83
191_R194_H0.5490.83
5_I86_A0.5470.83
41_I53_F0.5420.82
137_S167_T0.5400.82
87_M139_T0.5370.82
108_I174_I0.5350.81
111_I170_S0.5350.81
140_V169_F0.5300.81
57_Q60_V0.5280.81
143_V170_S0.5280.81
104_G182_C0.5230.80
67_V72_L0.5200.80
82_N168_I0.5200.80
109_T121_D0.5190.79
63_M116_N0.5190.79
182_C186_G0.5140.79
98_V103_D0.5130.79
96_H144_S0.5130.79
137_S161_P0.5100.78
69_A135_K0.5100.78
65_E68_K0.5060.78
39_D53_F0.5040.77
43_V60_V0.5030.77
129_T150_L0.5030.77
37_L52_D0.5030.77
113_E174_I0.5010.77
21_A63_M0.5000.77
28_C58_F0.5000.77
130_K195_K0.5000.77
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1kzlA 1 0.9302 100 0.111 Contact Map
4g6iA 3 0.9116 100 0.122 Contact Map
1i8dA 3 0.9302 100 0.126 Contact Map
3a35A 1 0.8512 100 0.171 Contact Map
3ddyA 1 0.8093 100 0.182 Contact Map
4tkoB 1 0.5814 47 0.955 Contact Map
2wg5A 3 0.2558 28.3 0.96 Contact Map
4nbqA 3 0.4326 25.5 0.961 Contact Map
3h43A 5 0.2698 22.5 0.962 Contact Map
2f1mA 2 0.6093 20.2 0.963 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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