SACY - BACSU - GREMLIN Contacts

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SACY - Levansucrase and sucrase synthesis operon antiterminator

UniProt:	P15401	Sequences: MKIKRILNHNAIVVKDQNEEKILLGAGIAFNKKKNDIVDPSKIEKTFIRKDTPDYKQFEEILETLPEDHIQISEQIISHAEKELNIKINERIHVAFSDHLSFAIERLSNGMVIKNPLLNEIKVLYPKEFQIGLWARALIKDKLGIHIPDDEIGNIAMHIHTARNNAGDMTQTLDITTMIRDIIEIIEIQLSINIVEDTISYERLVTHLRFAIQHIKAGESIYELDAEMIDIIKEKFKDAFLCALSIGTFVKKEYGFEFPEKELCYIAMHIQRFYQRSVAR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	280 (269)
Sequences:	3627 (3116)
Seq/√Len:	190.0

ECOLI - BGLG FRVR CSIE
BACSU - SACY SACT GLCT LICT MANR MTLR LICR

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
140_K	146_H	4.770	1.00
83_E	134_W	4.321	1.00
251_K	257_E	3.275	1.00
189_Q	245_S	3.113	1.00
233_K	240_F	3.067	1.00
187_E	193_N	3.055	1.00
229_I	264_C	3.052	1.00
233_K	260_E	3.009	1.00
105_E	109_N	2.990	1.00
10_N	13_V	2.973	1.00
239_A	271_Q	2.925	1.00
181_D	253_E	2.667	1.00
75_Q	142_K	2.636	1.00
243_A	267_A	2.495	1.00
186_I	204_L	2.490	1.00
133_L	152_I	2.360	1.00
14_V	21_K	2.340	1.00
197_D	276_R	2.276	1.00
185_I	249_F	2.261	1.00
76_I	135_A	2.205	1.00
99_H	158_H	2.204	1.00
214_H	262_E	2.130	1.00
79_H	138_L	2.130	1.00
243_A	263_L	2.104	1.00
218_G	256_F	2.055	1.00
4_K	15_K	2.046	1.00
7_L	13_V	1.976	1.00
27_G	31_N	1.962	1.00
244_L	260_E	1.960	1.00
180_R	201_Y	1.955	1.00
133_L	149_D	1.953	1.00
247_G	263_L	1.902	1.00
186_I	270_I	1.901	1.00
239_A	243_A	1.892	1.00
130_Q	133_L	1.833	1.00
11_A	14_V	1.822	1.00
80_A	135_A	1.770	1.00
79_H	134_W	1.759	1.00
11_A	15_K	1.747	1.00
182_I	246_I	1.732	1.00
207_H	269_H	1.716	1.00
107_L	147_I	1.692	1.00
190_L	242_C	1.686	1.00
239_A	270_I	1.684	1.00
186_I	242_C	1.654	1.00
6_I	33_K	1.653	1.00
184_E	188_I	1.602	1.00
118_L	153_G	1.597	1.00
241_L	244_L	1.589	1.00
231_I	235_K	1.577	1.00
136_R	140_K	1.553	1.00
197_D	273_F	1.552	1.00
212_I	216_K	1.547	1.00
11_A	16_D	1.544	1.00
66_P	69_H	1.527	1.00
95_A	158_H	1.526	1.00
248_T	251_K	1.504	1.00
107_L	148_P	1.503	1.00
232_I	267_A	1.495	1.00
244_L	263_L	1.478	1.00
247_G	266_I	1.471	1.00
200_S	276_R	1.446	1.00
243_A	247_G	1.427	1.00
12_I	15_K	1.412	1.00
132_G	152_I	1.405	1.00
81_E	88_I	1.405	1.00
226_A	264_C	1.400	1.00
211_A	215_I	1.385	1.00
200_S	273_F	1.382	1.00
76_I	80_A	1.379	1.00
183_I	201_Y	1.373	1.00
229_I	260_E	1.372	1.00
26_A	29_A	1.371	1.00
80_A	131_I	1.352	1.00
104_I	108_S	1.343	1.00
225_D	261_K	1.325	1.00
69_H	73_S	1.321	1.00
72_I	142_K	1.296	1.00
230_D	234_E	1.294	1.00
227_E	261_K	1.275	1.00
128_E	156_A	1.275	1.00
69_H	101_S	1.254	1.00
91_R	95_A	1.253	1.00
32_K	36_D	1.235	1.00
136_R	152_I	1.226	1.00
194_I	270_I	1.222	1.00
221_I	265_Y	1.214	1.00
104_I	147_I	1.213	1.00
77_I	97_S	1.204	1.00
211_A	259_P	1.185	1.00
10_N	14_V	1.185	1.00
12_I	16_D	1.184	1.00
148_P	151_E	1.182	1.00
95_A	99_H	1.181	1.00
186_I	243_A	1.171	1.00
16_D	19_E	1.157	1.00
177_T	253_E	1.157	1.00
244_L	248_T	1.148	1.00
194_I	273_F	1.147	1.00
208_L	211_A	1.135	1.00
240_F	267_A	1.131	1.00
243_A	270_I	1.127	1.00
132_G	135_A	1.111	1.00
226_A	261_K	1.109	1.00
45_K	49_R	1.097	1.00
261_K	264_C	1.093	1.00
181_D	249_F	1.087	1.00
72_I	143_L	1.082	1.00
129_F	152_I	1.081	1.00
179_I	205_V	1.076	1.00
132_G	156_A	1.067	1.00
240_F	260_E	1.066	1.00
137_A	146_H	1.064	1.00
185_I	188_I	1.064	1.00
240_F	244_L	1.062	1.00
134_W	138_L	1.054	1.00
129_F	156_A	1.054	1.00
101_S	105_E	1.044	1.00
13_V	16_D	1.038	1.00
100_L	155_I	1.035	1.00
243_A	246_I	1.034	1.00
27_G	30_F	1.032	1.00
100_L	139_I	1.028	1.00
17_Q	20_E	1.025	1.00
69_H	72_I	1.023	1.00
93_H	97_S	1.017	1.00
246_I	249_F	0.997	0.99
84_L	134_W	0.996	0.99
250_V	254_Y	0.992	0.99
243_A	266_I	0.987	0.99
246_I	270_I	0.986	0.99
201_Y	205_V	0.986	0.99
69_H	104_I	0.986	0.99
264_C	267_A	0.985	0.99
229_I	240_F	0.983	0.99
79_H	83_E	0.983	0.99
140_K	143_L	0.976	0.99
56_K	59_E	0.972	0.99
239_A	267_A	0.970	0.99
73_S	77_I	0.963	0.99
81_E	84_L	0.963	0.99
39_D	42_K	0.955	0.99
20_E	27_G	0.955	0.99
211_A	258_F	0.954	0.99
211_A	214_H	0.953	0.99
129_F	153_G	0.947	0.99
175_I	212_I	0.932	0.99
92_I	162_A	0.929	0.99
82_K	85_N	0.928	0.99
127_K	130_Q	0.926	0.99
134_W	137_A	0.925	0.99
273_F	276_R	0.925	0.99
27_G	33_K	0.922	0.99
159_I	163_R	0.922	0.99
112_V	148_P	0.920	0.99
118_L	154_N	0.919	0.99
80_A	159_I	0.918	0.99
247_G	258_F	0.914	0.99
182_I	270_I	0.914	0.99
126_P	129_F	0.914	0.99
249_F	253_E	0.912	0.99
71_Q	74_E	0.908	0.99
78_S	93_H	0.907	0.99
140_K	147_I	0.905	0.99
4_K	16_D	0.904	0.99
76_I	138_L	0.899	0.99
12_I	29_A	0.894	0.99
71_Q	75_Q	0.890	0.99
227_E	231_I	0.887	0.99
72_I	138_L	0.885	0.99
232_I	264_C	0.883	0.99
27_G	34_K	0.883	0.99
133_L	137_A	0.875	0.99
179_I	182_I	0.874	0.99
74_E	94_V	0.873	0.99
187_E	197_D	0.872	0.99
72_I	76_I	0.872	0.99
182_I	249_F	0.869	0.99
74_E	93_H	0.864	0.99
181_D	184_E	0.860	0.98
156_A	160_H	0.860	0.98
186_I	190_L	0.860	0.98
116_P	157_M	0.858	0.98
10_N	16_D	0.857	0.98
187_E	194_I	0.854	0.98
74_E	78_S	0.852	0.98
204_L	266_I	0.852	0.98
190_L	194_I	0.850	0.98
265_Y	269_H	0.846	0.98
248_T	252_K	0.843	0.98
179_I	208_L	0.843	0.98
242_C	246_I	0.843	0.98
178_M	182_I	0.841	0.98
28_I	35_N	0.841	0.98
185_I	189_Q	0.840	0.98
31_N	34_K	0.839	0.98
30_F	35_N	0.836	0.98
26_A	30_F	0.836	0.98
20_E	45_K	0.836	0.98
135_A	138_L	0.835	0.98
139_I	147_I	0.833	0.98
212_I	254_Y	0.833	0.98
129_F	133_L	0.832	0.98
189_Q	242_C	0.829	0.98
203_R	272_R	0.827	0.98
10_N	15_K	0.823	0.98
271_Q	274_Y	0.820	0.98
138_L	141_D	0.819	0.98
14_V	23_L	0.818	0.98
136_R	155_I	0.812	0.98
14_V	22_I	0.811	0.98
16_D	21_K	0.805	0.98
210_F	213_Q	0.803	0.98
99_H	103_A	0.799	0.98
203_R	276_R	0.794	0.97
88_I	159_I	0.792	0.97
172_T	176_T	0.790	0.97
269_H	272_R	0.785	0.97
151_E	265_Y	0.780	0.97
207_H	265_Y	0.774	0.97
136_R	139_I	0.768	0.97
238_D	241_L	0.767	0.97
238_D	243_A	0.764	0.97
20_E	35_N	0.762	0.97
113_I	151_E	0.762	0.97
40_P	46_T	0.760	0.97
215_I	256_F	0.759	0.97
17_Q	21_K	0.758	0.97
185_I	246_I	0.755	0.97
199_I	203_R	0.751	0.96
20_E	31_N	0.747	0.96
27_G	106_R	0.745	0.96
171_Q	174_D	0.744	0.96
73_S	100_L	0.741	0.96
226_A	263_L	0.731	0.96
80_A	138_L	0.730	0.96
106_R	154_N	0.730	0.96
180_R	184_E	0.725	0.96
240_F	243_A	0.723	0.96
206_T	210_F	0.723	0.96
162_A	211_A	0.722	0.96
178_M	254_Y	0.721	0.96
227_E	235_K	0.717	0.95
209_R	213_Q	0.715	0.95
251_K	254_Y	0.715	0.95
135_A	159_I	0.714	0.95
253_E	256_F	0.714	0.95
103_A	107_L	0.713	0.95
121_I	153_G	0.711	0.95
250_V	256_F	0.710	0.95
203_R	273_F	0.704	0.95
6_I	34_K	0.704	0.95
241_L	245_S	0.704	0.95
17_Q	22_I	0.701	0.95
65_L	101_S	0.699	0.95
86_I	268_M	0.696	0.95
108_S	144_G	0.694	0.94
11_A	25_G	0.693	0.94
239_A	274_Y	0.692	0.94
272_R	276_R	0.692	0.94
80_A	134_W	0.687	0.94
183_I	270_I	0.686	0.94
73_S	97_S	0.681	0.94
240_F	263_L	0.679	0.94
86_I	163_R	0.677	0.94
29_A	32_K	0.676	0.94
68_D	142_K	0.675	0.94
176_T	205_V	0.673	0.94
227_E	264_C	0.673	0.94
178_M	208_L	0.671	0.93
30_F	33_K	0.670	0.93
42_K	46_T	0.670	0.93
73_S	76_I	0.668	0.93
213_Q	217_A	0.667	0.93
78_S	82_K	0.663	0.93
179_I	183_I	0.661	0.93
33_K	37_I	0.658	0.93
105_E	108_S	0.655	0.93
236_F	271_Q	0.652	0.92
190_L	193_N	0.650	0.92
132_G	159_I	0.650	0.92
229_I	233_K	0.649	0.92
89_N	163_R	0.648	0.92
107_L	151_E	0.647	0.92
203_R	269_H	0.644	0.92
91_R	162_A	0.643	0.92
226_A	230_D	0.639	0.92
108_S	137_A	0.638	0.92
190_L	246_I	0.638	0.92
197_D	275_Q	0.638	0.92
54_D	57_Q	0.635	0.91
139_I	143_L	0.634	0.91
231_I	236_F	0.633	0.91
242_C	270_I	0.632	0.91
115_N	153_G	0.628	0.91
67_E	71_Q	0.626	0.91
169_M	172_T	0.625	0.91
161_T	210_F	0.623	0.91
88_I	92_I	0.621	0.91
200_S	204_L	0.620	0.90
16_D	20_E	0.619	0.90
120_E	153_G	0.616	0.90
120_E	149_D	0.616	0.90
231_I	234_E	0.614	0.90
155_I	158_H	0.612	0.90
84_L	128_E	0.610	0.90
233_K	264_C	0.609	0.90
227_E	232_I	0.608	0.90
129_F	149_D	0.608	0.90
137_A	141_D	0.608	0.90
78_S	81_E	0.607	0.90
183_I	200_S	0.604	0.89
80_A	84_L	0.604	0.89
176_T	201_Y	0.597	0.89
184_E	195_V	0.596	0.89
99_H	151_E	0.595	0.89
132_G	155_I	0.593	0.88
99_H	203_R	0.592	0.88
250_V	258_F	0.591	0.88
165_N	266_I	0.589	0.88
119_N	123_V	0.588	0.88
232_I	268_M	0.587	0.88
115_N	121_I	0.585	0.88
214_H	221_I	0.585	0.88
150_D	153_G	0.585	0.88
35_N	38_V	0.585	0.88
115_N	120_E	0.583	0.88
107_L	112_V	0.582	0.88
204_L	273_F	0.581	0.87
84_L	88_I	0.580	0.87
115_N	150_D	0.579	0.87
69_H	108_S	0.579	0.87
106_R	151_E	0.578	0.87
228_M	231_I	0.576	0.87
31_N	35_N	0.574	0.87
84_L	131_I	0.573	0.87
10_N	29_A	0.573	0.87
92_I	211_A	0.573	0.87
104_I	139_I	0.572	0.87
185_I	245_S	0.569	0.86
15_K	24_L	0.569	0.86
86_I	127_K	0.569	0.86
175_I	205_V	0.568	0.86
229_I	261_K	0.565	0.86
158_H	210_F	0.565	0.86
20_E	34_K	0.563	0.86
126_P	156_A	0.562	0.86
212_I	256_F	0.560	0.85
199_I	204_L	0.557	0.85
183_I	186_I	0.554	0.85
75_Q	79_H	0.553	0.85
19_E	264_C	0.553	0.85
133_L	146_H	0.551	0.85
182_I	204_L	0.551	0.85
244_L	247_G	0.550	0.84
232_I	263_L	0.550	0.84
103_A	148_P	0.548	0.84
25_G	31_N	0.547	0.84
215_I	259_P	0.547	0.84
129_F	160_H	0.542	0.84
240_F	271_Q	0.542	0.84
80_A	132_G	0.541	0.83
77_I	135_A	0.540	0.83
158_H	161_T	0.538	0.83
76_I	139_I	0.537	0.83
22_I	27_G	0.536	0.83
50_K	90_E	0.534	0.83
76_I	79_H	0.532	0.82
11_A	29_A	0.531	0.82
33_K	36_D	0.531	0.82
19_E	70_I	0.531	0.82
271_Q	275_Q	0.531	0.82
183_I	187_E	0.529	0.82
79_H	131_I	0.529	0.82
152_I	156_A	0.529	0.82
117_L	120_E	0.528	0.82
15_K	40_P	0.528	0.82
36_D	40_P	0.528	0.82
221_I	261_K	0.527	0.82
114_K	150_D	0.527	0.82
137_A	140_K	0.526	0.82
199_I	210_F	0.526	0.82
131_I	159_I	0.524	0.82
104_I	145_I	0.523	0.81
73_S	139_I	0.519	0.81
179_I	201_Y	0.517	0.81
88_I	163_R	0.517	0.81
20_E	33_K	0.516	0.81
210_F	217_A	0.515	0.80
92_I	97_S	0.515	0.80
233_K	236_F	0.514	0.80
77_I	92_I	0.513	0.80
187_E	192_I	0.512	0.80
138_L	142_K	0.511	0.80
182_I	243_A	0.510	0.80
229_I	275_Q	0.510	0.80
209_R	271_Q	0.509	0.80
236_F	265_Y	0.507	0.79
187_E	195_V	0.506	0.79
269_H	276_R	0.505	0.79
241_L	248_T	0.505	0.79
215_I	262_E	0.504	0.79
186_I	189_Q	0.502	0.79
25_G	30_F	0.500	0.78

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4r6iA	2	0.9107	100	0.333	Contact Map
1h99A	2	0.7821	100	0.335	Contact Map
3sqnA	2	0.8893	100	0.442	Contact Map
3rioA	1	0.5893	99.9	0.595	Contact Map
3ufeA	2	0.3821	99.7	0.656	Contact Map
3nufA	2	0.4214	89.5	0.904	Contact Map
1auuA	2	0.1964	84.6	0.911	Contact Map
1l1cA	2	0.1929	84.1	0.912	Contact Map
1wwpA	2	0.2607	5	0.958	Contact Map
1wtyA	4	0.2643	4.9	0.958	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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