GREMLIN Database
SACX - Negative regulator of SacY activity
UniProt: P15400 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 459 (360)
Sequences: 4552 (2589)
Seq/√Len: 136.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
401_Y440_I4.0171.00
394_G444_S3.8181.00
298_G396_A3.7671.00
226_S262_A3.6501.00
347_A446_F3.3921.00
230_M254_T3.2711.00
253_I257_V3.2581.00
401_Y433_H2.9611.00
164_G178_G2.8261.00
397_I444_S2.6421.00
341_Q381_N2.5131.00
390_G452_L2.5011.00
98_L103_A2.4981.00
241_V249_I2.4901.00
351_K453_G2.4831.00
435_M439_L2.4771.00
401_Y405_V2.3811.00
449_T453_G2.3691.00
350_L449_T2.3591.00
389_I393_I2.3551.00
165_F179_A2.3411.00
165_F175_P2.2921.00
343_G441_A2.2661.00
128_L132_I2.2461.00
283_Y287_H2.2261.00
236_L240_I2.2261.00
345_G376_I2.2061.00
405_V433_H2.1991.00
238_K246_D2.1731.00
319_L328_N2.1641.00
229_V261_L2.1251.00
352_T357_L2.0751.00
161_I183_G2.0661.00
237_L253_I2.0631.00
447_I451_F2.0241.00
297_G395_G1.9571.00
272_I276_I1.9341.00
349_F362_L1.8941.00
132_I137_W1.8751.00
294_A400_A1.8521.00
343_G445_A1.8461.00
350_L445_A1.8451.00
354_Q357_L1.8381.00
232_K235_K1.8291.00
390_G394_G1.8051.00
107_T171_F1.7921.00
433_H436_I1.7891.00
126_M153_S1.7881.00
402_V437_G1.7751.00
245_L249_I1.7711.00
342_G368_A1.7681.00
390_G448_A1.7611.00
275_Q279_Q1.7451.00
153_S157_I1.7321.00
448_A452_L1.7301.00
377_V388_F1.7181.00
302_T392_A1.6931.00
161_I179_A1.6921.00
343_G442_A1.6911.00
400_A404_A1.6881.00
384_L387_P1.6861.00
225_L270_A1.6851.00
443_V447_I1.6821.00
444_S448_A1.6791.00
237_L241_V1.6451.00
232_K236_L1.6391.00
173_S227_V1.6321.00
228_F231_S1.6231.00
348_V361_A1.6161.00
233_I258_T1.6141.00
298_G302_T1.6131.00
346_L365_S1.6081.00
100_P103_A1.5651.00
322_N325_V1.5621.00
169_R175_P1.5611.00
327_V408_V1.5391.00
293_G399_G1.5281.00
280_S283_Y1.5141.00
297_G399_G1.5041.00
294_A396_A1.4981.00
228_F232_K1.4951.00
249_I253_I1.4801.00
182_A254_T1.4711.00
106_K170_Q1.4611.00
229_V258_T1.4581.00
357_L384_L1.4351.00
168_A227_V1.4351.00
165_F176_Y1.4181.00
434_Y438_F1.4121.00
394_G447_I1.4091.00
343_G347_A1.4031.00
258_T262_A1.3981.00
386_R455_K1.3931.00
290_I400_A1.3921.00
167_A228_F1.3871.00
233_I257_V1.3871.00
354_Q358_K1.3761.00
276_I280_S1.3761.00
446_F450_L1.3711.00
346_L350_L1.3661.00
318_T322_N1.3611.00
348_V352_T1.3521.00
100_P104_F1.3441.00
384_L454_F1.3381.00
282_V286_D1.3031.00
124_L252_F1.3021.00
397_I440_I1.2831.00
300_Y333_I1.2601.00
365_S369_F1.2571.00
166_S176_Y1.2531.00
293_G403_V1.2481.00
165_F180_V1.2391.00
178_G182_A1.2391.00
401_Y437_G1.2391.00
246_D250_I1.2381.00
401_Y436_I1.2371.00
301_S338_N1.2351.00
125_L152_S1.2341.00
394_G445_A1.2271.00
398_G440_I1.2261.00
335_S434_Y1.2251.00
151_V259_G1.2221.00
147_M211_I1.2181.00
293_G400_A1.2131.00
237_L250_I1.2051.00
273_I276_I1.2001.00
432_V435_M1.1961.00
229_V266_M1.1911.00
277_M303_I1.1901.00
402_V407_V1.1891.00
290_I404_A1.1840.99
298_G389_I1.1730.99
181_I255_V1.1710.99
163_V220_M1.1680.99
122_S155_A1.1600.99
221_I225_L1.1560.99
276_I279_Q1.1550.99
377_V391_A1.1520.99
336_M395_G1.1500.99
112_F220_M1.1480.99
168_A175_P1.1430.99
168_A178_G1.1300.99
303_I312_F1.1300.99
351_K449_T1.1230.99
363_P367_T1.1130.99
347_A450_L1.1130.99
129_I145_I1.1080.99
115_I166_S1.0970.99
438_F442_A1.0940.99
340_A391_A1.0850.99
386_R453_G1.0820.99
336_M402_V1.0770.99
231_S235_K1.0740.99
265_V269_A1.0670.99
184_L259_G1.0600.99
173_S230_M1.0590.99
394_G398_G1.0570.99
227_V231_S1.0480.99
168_A173_S1.0480.99
381_N387_P1.0420.99
420_I435_M1.0340.99
294_A397_I1.0330.99
393_I448_A1.0310.99
401_Y404_A1.0300.99
340_A344_A1.0290.99
181_I223_I1.0280.99
351_K454_F1.0230.99
350_L446_F1.0220.99
233_I237_L1.0200.98
349_F361_A1.0170.98
226_S258_T1.0140.98
270_A274_G1.0140.98
96_R100_P1.0090.98
281_I329_F1.0080.98
172_G235_K1.0080.98
390_G447_I1.0070.98
182_A223_I1.0070.98
174_N234_E1.0000.98
439_L443_V0.9970.98
398_G441_A0.9950.98
171_F228_F0.9940.98
110_D170_Q0.9940.98
154_T183_G0.9900.98
158_L188_P0.9900.98
139_A142_S0.9870.98
438_F441_A0.9860.98
298_G392_A0.9850.98
167_A224_L0.9850.98
123_G412_Y0.9830.98
242_P245_L0.9770.98
96_R99_N0.9730.98
241_V245_L0.9710.98
413_G422_I0.9700.98
180_V184_L0.9700.98
341_Q391_A0.9700.98
391_A444_S0.9680.98
164_G223_I0.9670.98
288_A291_A0.9660.98
161_I180_V0.9620.98
350_L453_G0.9570.98
335_S419_M0.9510.98
432_V436_I0.9500.98
115_I159_L0.9480.98
163_V223_I0.9470.98
107_T111_I0.9470.98
236_L239_S0.9470.98
450_L454_F0.9420.97
181_I258_T0.9360.97
312_F316_E0.9350.97
344_A381_N0.9270.97
243_K246_D0.9260.97
357_L380_V0.9260.97
369_F417_I0.9230.97
430_N433_H0.9180.97
226_S261_L0.9150.97
257_V260_C0.9130.97
269_A273_I0.9130.97
146_K149_D0.9100.97
339_V342_G0.9070.97
144_W147_M0.9040.97
186_T217_Q0.9040.97
344_A391_A0.9020.97
173_S178_G0.9020.97
296_F331_V0.9010.97
329_F332_P0.8990.97
126_M156_F0.8970.97
302_T388_F0.8940.97
240_I249_I0.8910.96
129_I152_S0.8890.96
266_M270_A0.8890.96
440_I443_V0.8820.96
216_Y222_P0.8820.96
221_I270_A0.8790.96
387_P390_G0.8780.96
130_G149_D0.8780.96
405_V436_I0.8770.96
349_F358_K0.8760.96
279_Q282_V0.8710.96
101_A104_F0.8710.96
97_K103_A0.8690.96
450_L453_G0.8630.96
260_C265_V0.8630.96
357_L456_E0.8620.96
238_K250_I0.8600.96
390_G451_F0.8560.96
440_I444_S0.8550.96
421_S425_P0.8540.95
341_Q376_I0.8530.95
390_G449_T0.8520.95
155_A162_L0.8500.95
442_A445_A0.8480.95
368_A373_V0.8480.95
93_K96_R0.8470.95
200_T203_S0.8460.95
261_L266_M0.8450.95
407_V437_G0.8410.95
256_M260_C0.8370.95
381_N391_A0.8370.95
373_V376_I0.8370.95
381_N388_F0.8360.95
164_G227_V0.8330.95
225_L266_M0.8310.95
164_G182_A0.8310.95
165_F169_R0.8300.95
263_L268_P0.8290.95
104_F107_T0.8260.95
272_I275_Q0.8260.95
162_L180_V0.8240.95
229_V233_I0.8220.94
325_V329_F0.8210.94
96_R103_A0.8210.94
106_K110_D0.8190.94
223_I227_V0.8160.94
387_P449_T0.8160.94
111_I224_L0.8160.94
151_V184_L0.8070.94
171_F231_S0.8060.94
107_T228_F0.8050.94
162_L183_G0.8050.94
241_V250_I0.7970.94
191_L195_M0.7970.94
441_A445_A0.7970.94
337_A341_Q0.7960.93
442_A446_F0.7930.93
405_V440_I0.7890.93
397_I401_Y0.7880.93
239_S353_K0.7850.93
206_I210_H0.7850.93
351_K450_L0.7840.93
348_V380_V0.7830.93
131_M135_F0.7800.93
358_K362_L0.7790.93
173_S234_E0.7780.93
365_S368_A0.7770.93
95_S103_A0.7750.93
302_T306_S0.7750.93
279_Q283_Y0.7740.92
229_V232_K0.7730.92
167_A171_F0.7720.92
117_P120_T0.7720.92
261_L265_V0.7710.92
144_W206_I0.7670.92
451_F455_K0.7660.92
142_S146_K0.7640.92
360_I364_A0.7590.92
135_F138_F0.7580.92
122_S154_T0.7580.92
195_M198_S0.7560.92
132_I138_F0.7540.91
352_T456_E0.7540.91
299_I302_T0.7540.91
290_I294_A0.7540.91
348_V387_P0.7540.91
341_Q344_A0.7500.91
113_V310_H0.7480.91
439_L447_I0.7480.91
172_G234_E0.7480.91
343_G369_F0.7470.91
154_T184_L0.7460.91
164_G179_A0.7450.91
304_V337_A0.7440.91
424_L430_N0.7410.91
269_A272_I0.7400.91
177_L230_M0.7380.91
407_V433_H0.7370.91
299_I312_F0.7330.90
306_S378_F0.7310.90
301_S377_V0.7310.90
293_G333_I0.7290.90
289_G292_A0.7290.90
318_T321_A0.7280.90
445_A453_G0.7260.90
283_Y289_G0.7250.90
172_G178_G0.7230.90
339_V438_F0.7220.90
192_D195_M0.7210.90
245_L370_L0.7110.89
386_R454_F0.7030.88
205_D209_L0.7020.88
131_M137_W0.6980.88
153_S187_H0.6960.88
355_S358_K0.6960.88
335_S402_V0.6950.88
344_A387_P0.6950.88
274_G308_L0.6940.88
389_I392_A0.6940.88
146_K150_L0.6910.88
417_I421_S0.6900.87
254_T258_T0.6880.87
294_A298_G0.6840.87
296_F312_F0.6840.87
341_Q368_A0.6830.87
221_I305_L0.6830.87
193_P204_L0.6810.87
362_L365_S0.6800.87
133_K145_I0.6790.87
340_A394_G0.6790.87
293_G297_G0.6770.86
406_Q429_A0.6770.86
295_L299_I0.6760.86
425_P430_N0.6760.86
182_A230_M0.6760.86
234_E250_I0.6750.86
344_A449_T0.6750.86
337_A340_A0.6750.86
161_I182_A0.6750.86
452_L456_E0.6750.86
190_L194_S0.6720.86
354_Q456_E0.6720.86
364_A367_T0.6710.86
131_M134_V0.6700.86
336_M398_G0.6690.86
394_G448_A0.6680.86
301_S337_A0.6680.86
159_L220_M0.6680.86
321_A325_V0.6660.86
295_L298_G0.6650.85
297_G396_A0.6650.85
99_N102_A0.6630.85
393_I396_A0.6630.85
95_S98_L0.6620.85
299_I392_A0.6600.85
339_V437_G0.6600.85
345_G365_S0.6600.85
397_I400_A0.6580.85
241_V253_I0.6570.85
407_V415_T0.6560.85
415_T419_M0.6550.85
149_D153_S0.6530.84
411_S422_I0.6450.84
122_S159_L0.6440.84
345_G349_F0.6430.84
383_K387_P0.6410.83
311_S314_A0.6410.83
380_V384_L0.6360.83
233_I254_T0.6330.83
296_F303_I0.6320.82
342_G345_G0.6300.82
133_K149_D0.6290.82
348_V357_L0.6280.82
339_V416_G0.6280.82
271_S275_Q0.6250.82
336_M340_A0.6250.82
247_V250_I0.6250.82
257_V261_L0.6250.82
407_V431_F0.6210.81
434_Y439_L0.6210.81
399_G403_V0.6170.81
227_V230_M0.6170.81
388_F391_A0.6170.81
429_A432_V0.6160.81
338_N373_V0.6160.81
394_G397_I0.6150.81
344_A348_V0.6120.80
317_A321_A0.6120.80
386_R390_G0.6080.80
370_L418_P0.6070.80
104_F110_D0.6060.80
135_F139_A0.6050.80
278_T312_F0.6050.80
162_L179_A0.6030.79
283_Y286_D0.6020.79
361_A365_S0.6000.79
346_L446_F0.5990.79
287_H291_A0.5980.79
111_I167_A0.5970.79
124_L127_G0.5950.79
288_A292_A0.5940.78
133_K137_W0.5940.78
126_M157_I0.5930.78
345_G361_A0.5920.78
243_K356_S0.5920.78
348_V351_K0.5910.78
346_L349_F0.5900.78
356_S360_I0.5880.78
108_L306_S0.5870.78
299_I303_I0.5870.78
347_A449_T0.5870.78
351_K386_R0.5870.78
178_G227_V0.5870.78
237_L240_I0.5850.77
119_I310_H0.5830.77
362_L366_L0.5810.77
386_R389_I0.5810.77
450_L455_K0.5810.77
116_I416_G0.5800.77
234_E238_K0.5800.77
351_K456_E0.5790.77
105_A382_L0.5790.77
168_A230_M0.5780.77
233_I253_I0.5770.76
129_I149_D0.5750.76
225_L228_F0.5750.76
325_V330_L0.5740.76
341_Q373_V0.5720.76
433_H440_I0.5720.76
326_G330_L0.5720.76
337_A415_T0.5710.76
359_K363_P0.5710.76
446_F449_T0.5680.75
181_I226_S0.5680.75
301_S388_F0.5680.75
410_N424_L0.5670.75
348_V384_L0.5670.75
317_A412_Y0.5670.75
294_A297_G0.5650.75
424_L431_F0.5650.75
196_L199_K0.5630.75
415_T422_I0.5630.75
127_G293_G0.5620.75
156_F159_L0.5580.74
326_G329_F0.5570.74
338_N365_S0.5570.74
427_G430_N0.5550.74
336_M441_A0.5550.74
118_A177_L0.5550.74
121_A255_V0.5550.74
313_Y316_E0.5540.74
150_L187_H0.5530.73
252_F255_V0.5520.73
418_P421_S0.5520.73
345_G381_N0.5520.73
357_L454_F0.5520.73
301_S373_V0.5510.73
435_M438_F0.5510.73
420_I424_L0.5510.73
190_L263_L0.5500.73
205_D208_G0.5500.73
298_G306_S0.5460.72
123_G314_A0.5460.72
157_I188_P0.5440.72
203_S212_P0.5440.72
212_P271_S0.5420.72
118_A180_V0.5410.72
112_F314_A0.5400.72
127_G416_G0.5380.71
157_I217_Q0.5370.71
296_F333_I0.5370.71
173_S254_T0.5370.71
367_T376_I0.5370.71
395_G399_G0.5360.71
387_P391_A0.5350.71
154_T187_H0.5350.71
104_F108_L0.5340.71
201_P204_L0.5330.71
416_G419_M0.5300.70
297_G333_I0.5270.70
342_G365_S0.5260.70
206_I209_L0.5260.70
128_L131_M0.5250.70
116_I371_G0.5250.70
123_G367_T0.5230.69
172_G175_P0.5230.69
338_N391_A0.5220.69
109_S310_H0.5190.69
125_L252_F0.5180.68
337_A391_A0.5180.68
308_L315_I0.5160.68
94_A103_A0.5130.68
337_A398_G0.5130.68
133_K138_F0.5130.68
175_P227_V0.5120.68
206_I211_I0.5110.67
133_K140_A0.5100.67
216_Y274_G0.5100.67
281_I315_I0.5090.67
141_G146_K0.5080.67
229_V257_V0.5080.67
212_P215_G0.5070.67
127_G388_F0.5070.67
108_L119_I0.5050.67
337_A373_V0.5050.67
202_S205_D0.5040.66
301_S341_Q0.5040.66
123_G127_G0.5040.66
119_I455_K0.5040.66
304_V341_Q0.5020.66
357_L361_A0.5020.66
226_S230_M0.5020.66
221_I277_M0.5000.66
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3qnqA 2 0.8061 100 0.648 Contact Map
3ipjA 1 0.1983 100 0.789 Contact Map
1ibaA 1 0.1678 100 0.794 Contact Map
3bp3A 1 0.1743 100 0.794 Contact Map
4bxoA 1 0.1176 53 0.971 Contact Map
1kftA 1 0.1024 39.1 0.973 Contact Map
4n8nA 1 0.1046 33.4 0.974 Contact Map
2a1jB 2 0.1133 31.7 0.975 Contact Map
3noyA 2 0.183 30.1 0.975 Contact Map
1x2iA 2 0.1111 27.5 0.975 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0109 seconds.