ALF - BACSU - GREMLIN Contacts

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ALF - Probable fructose-bisphosphate aldolase

UniProt:	P13243	Sequences: MPLVSMTEMLNTAKEKGYAVGQFNLNNLEFTQAILQAAEEEKSPVILGVSEGAGRYMGGFKTVVAMVKALMEEYKVTVPVAIHLDHGSSFESCAKAIHAGFTSVMIDASHHPFEENVATTAKVVELAHFHGVSVEAELGTVGGQEDDVIAEGVIYADPKECQELVERTGIDCLAPALGSVHGPYKGEPNLGFKEMEEIGKSTGLPLVLHGGTGIPTADIKKSISLGTAKINVNTENQISSAKAVRETLAAKPDEYDPRKYLGPAREAIKETVIGKMREFGSSNQA	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	285 (276)
Sequences:	3952 (2005)
Seq/√Len:	120.7

ECOLI - GATY KBAY YDJI ALF
BACSU - ALF IOLJ

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
163_E	167_R	3.976	1.00
40_E	273_I	3.395	1.00
40_E	269_K	3.339	1.00
12_T	16_K	3.050	1.00
113_F	163_E	2.997	1.00
41_E	277_R	2.753	1.00
43_S	281_S	2.684	1.00
113_F	117_V	2.575	1.00
162_Q	166_E	2.554	1.00
196_E	200_K	2.509	1.00
97_I	132_V	2.482	1.00
36_Q	269_K	2.437	1.00
246_E	250_A	2.411	1.00
39_E	77_T	2.399	1.00
122_K	125_E	2.393	1.00
112_P	115_E	2.379	1.00
94_A	126_L	2.364	1.00
191_G	194_E	2.363	1.00
106_I	119_T	2.266	1.00
216_T	278_E	2.265	1.00
177_L	195_M	2.252	1.00
34_I	45_V	2.226	1.00
118_A	122_K	2.206	1.00
49_V	101_F	2.197	1.00
12_T	18_Y	2.135	1.00
41_E	273_I	2.131	1.00
6_M	81_A	2.066	1.00
243_A	267_A	2.032	1.00
128_H	171_D	1.978	1.00
41_E	282_S	1.972	1.00
98_H	126_L	1.970	1.00
9_M	81_A	1.951	1.00
196_E	225_L	1.895	1.00
192_F	221_K	1.884	1.00
121_A	125_E	1.881	1.00
215_P	218_D	1.872	1.00
266_E	270_E	1.869	1.00
269_K	273_I	1.855	1.00
11_N	15_E	1.844	1.00
162_Q	201_S	1.816	1.00
7_T	11_N	1.790	1.00
277_R	282_S	1.784	1.00
14_K	228_A	1.758	1.00
221_K	224_S	1.746	1.00
6_M	172_C	1.726	1.00
115_E	118_A	1.715	1.00
94_A	98_H	1.672	1.00
35_L	76_V	1.656	1.00
108_A	111_H	1.645	1.00
22_Q	48_G	1.632	1.00
124_V	134_V	1.623	1.00
243_A	246_E	1.577	1.00
160_E	163_E	1.544	1.00
98_H	130_H	1.531	1.00
159_K	201_S	1.530	1.00
45_V	80_V	1.521	1.00
192_F	225_L	1.520	1.00
196_E	221_K	1.511	1.00
276_M	282_S	1.510	1.00
25_L	47_L	1.505	1.00
114_E	167_R	1.504	1.00
83_H	103_S	1.486	1.00
111_H	115_E	1.473	1.00
126_L	130_H	1.466	1.00
206_L	227_T	1.462	1.00
221_K	225_L	1.455	1.00
14_K	199_G	1.439	1.00
270_E	273_I	1.427	1.00
242_K	246_E	1.419	1.00
31_T	70_L	1.415	1.00
35_L	80_V	1.407	1.00
32_Q	265_R	1.403	1.00
39_E	76_V	1.354	1.00
68_K	72_E	1.350	1.00
197_E	200_K	1.349	1.00
21_G	272_V	1.342	1.00
113_F	160_E	1.325	1.00
157_D	160_E	1.325	1.00
267_A	270_E	1.311	1.00
216_T	274_G	1.299	1.00
247_T	251_K	1.267	1.00
179_S	214_I	1.267	1.00
65_A	68_K	1.255	0.99
245_R	248_L	1.246	0.99
90_F	94_A	1.244	0.99
31_T	67_V	1.244	0.99
25_L	31_T	1.240	0.99
224_S	280_G	1.231	0.99
65_A	69_A	1.231	0.99
271_T	274_G	1.223	0.99
194_E	198_I	1.219	0.99
90_F	122_K	1.218	0.99
263_P	266_E	1.211	0.99
113_F	167_R	1.198	0.99
25_L	34_I	1.193	0.99
51_E	92_S	1.193	0.99
17_G	224_S	1.189	0.99
37_A	272_V	1.185	0.99
125_E	129_F	1.178	0.99
42_K	77_T	1.170	0.99
244_V	264_A	1.170	0.99
223_I	279_F	1.168	0.99
216_T	220_K	1.167	0.99
62_T	70_L	1.159	0.99
64_V	82_I	1.158	0.99
37_A	41_E	1.152	0.99
111_H	119_T	1.149	0.99
120_T	136_A	1.146	0.99
38_A	45_V	1.140	0.99
68_K	82_I	1.139	0.99
61_K	72_E	1.123	0.99
123_V	134_V	1.117	0.99
121_A	167_R	1.112	0.99
5_S	133_S	1.110	0.99
194_E	197_E	1.110	0.99
114_E	163_E	1.103	0.99
233_N	237_Q	1.098	0.99
197_E	201_S	1.087	0.98
119_T	122_K	1.085	0.98
36_Q	265_R	1.085	0.98
97_I	100_G	1.084	0.98
61_K	95_K	1.075	0.98
104_V	134_V	1.073	0.98
270_E	274_G	1.069	0.98
114_E	118_A	1.068	0.98
104_V	127_A	1.066	0.98
165_V	204_L	1.063	0.98
104_V	123_V	1.056	0.98
61_K	73_E	1.050	0.98
117_V	167_R	1.050	0.98
193_K	197_E	1.044	0.98
94_A	130_H	1.041	0.98
247_T	254_E	1.041	0.98
37_A	269_K	1.040	0.98
97_I	130_H	1.033	0.98
97_I	126_L	1.030	0.98
273_I	277_R	1.022	0.98
69_A	72_E	1.012	0.98
202_T	206_L	1.012	0.98
175_P	198_I	1.001	0.97
108_A	119_T	1.000	0.97
21_G	276_M	0.997	0.97
8_E	12_T	0.997	0.97
12_T	15_E	0.990	0.97
161_C	202_T	0.989	0.97
91_E	95_K	0.988	0.97
266_E	269_K	0.987	0.97
158_P	201_S	0.985	0.97
199_G	228_A	0.985	0.97
49_V	63_V	0.980	0.97
33_A	268_I	0.976	0.97
193_K	196_E	0.973	0.97
90_F	93_C	0.964	0.97
26_N	53_A	0.961	0.97
37_A	273_I	0.958	0.97
21_G	279_F	0.957	0.97
239_S	271_T	0.956	0.97
251_K	254_E	0.951	0.96
230_I	279_F	0.949	0.96
76_V	80_V	0.947	0.96
274_G	278_E	0.944	0.96
9_M	20_V	0.941	0.96
38_A	276_M	0.938	0.96
150_A	153_V	0.935	0.96
232_V	279_F	0.934	0.96
191_G	197_E	0.932	0.96
26_N	30_F	0.916	0.96
10_L	172_C	0.915	0.96
102_T	133_S	0.915	0.96
136_A	164_L	0.907	0.95
244_V	260_Y	0.907	0.95
31_T	47_L	0.901	0.95
97_I	127_A	0.901	0.95
220_K	224_S	0.892	0.95
115_E	119_T	0.886	0.95
155_Y	194_E	0.885	0.95
136_A	170_I	0.884	0.95
93_C	97_I	0.883	0.95
158_P	197_E	0.882	0.95
17_G	228_A	0.881	0.95
50_S	53_A	0.870	0.94
71_M	76_V	0.870	0.94
192_F	218_D	0.867	0.94
144_Q	149_I	0.865	0.94
34_I	272_V	0.857	0.94
18_Y	43_S	0.856	0.94
64_V	68_K	0.852	0.94
43_S	280_G	0.851	0.94
177_L	208_L	0.850	0.94
272_V	276_M	0.849	0.94
88_S	110_H	0.849	0.94
208_L	222_S	0.847	0.93
90_F	100_G	0.846	0.93
96_A	100_G	0.844	0.93
219_I	279_F	0.843	0.93
67_V	82_I	0.836	0.93
183_P	238_I	0.834	0.93
219_I	223_I	0.834	0.93
44_P	79_P	0.827	0.93
5_S	8_E	0.826	0.93
120_T	124_V	0.823	0.92
10_L	20_V	0.819	0.92
263_P	267_A	0.815	0.92
92_S	96_A	0.813	0.92
161_C	198_I	0.812	0.92
126_L	129_F	0.812	0.92
143_G	146_D	0.810	0.92
93_C	96_A	0.809	0.92
38_A	78_V	0.808	0.92
254_E	259_K	0.806	0.92
249_A	252_P	0.805	0.92
51_E	89_S	0.802	0.91
91_E	94_A	0.796	0.91
37_A	276_M	0.795	0.91
23_F	45_V	0.794	0.91
23_F	272_V	0.793	0.91
57_M	63_V	0.791	0.91
27_N	30_F	0.789	0.91
94_A	97_I	0.788	0.91
51_E	88_S	0.788	0.91
48_G	233_N	0.787	0.91
179_S	190_L	0.785	0.91
39_E	78_V	0.782	0.90
156_A	161_C	0.779	0.90
50_S	233_N	0.776	0.90
61_K	65_A	0.773	0.90
128_H	131_G	0.773	0.90
276_M	281_S	0.773	0.90
104_V	132_V	0.773	0.90
219_I	230_I	0.770	0.90
5_S	131_G	0.769	0.90
215_P	219_I	0.767	0.90
165_V	202_T	0.767	0.90
48_G	83_H	0.765	0.89
163_E	166_E	0.761	0.89
145_E	148_V	0.761	0.89
93_C	100_G	0.761	0.89
127_A	132_V	0.760	0.89
62_T	66_M	0.759	0.89
220_K	280_G	0.754	0.89
246_E	249_A	0.754	0.89
159_K	162_Q	0.753	0.89
90_F	126_L	0.753	0.89
28_L	66_M	0.752	0.89
49_V	54_G	0.751	0.89
195_M	227_T	0.750	0.88
235_E	275_K	0.745	0.88
53_A	233_N	0.745	0.88
138_L	165_V	0.744	0.88
156_A	175_P	0.744	0.88
168_T	171_D	0.735	0.87
106_I	123_V	0.734	0.87
123_V	126_L	0.733	0.87
112_P	118_A	0.730	0.87
87_G	93_C	0.729	0.87
89_S	92_S	0.729	0.87
147_D	151_E	0.728	0.87
23_F	34_I	0.728	0.87
198_I	202_T	0.724	0.87
152_G	157_D	0.723	0.87
24_N	50_S	0.717	0.86
256_D	259_K	0.717	0.86
195_M	225_L	0.716	0.86
156_A	198_I	0.715	0.86
184_Y	188_P	0.713	0.86
6_M	133_S	0.710	0.86
124_V	128_H	0.710	0.86
8_E	11_N	0.702	0.85
236_N	268_I	0.693	0.84
138_L	164_L	0.693	0.84
140_T	153_V	0.690	0.84
11_N	14_K	0.682	0.83
223_I	226_G	0.682	0.83
106_I	111_H	0.680	0.83
96_A	101_F	0.679	0.83
243_A	264_A	0.678	0.83
81_A	133_S	0.678	0.83
217_A	220_K	0.676	0.83
138_L	161_C	0.675	0.83
245_R	249_A	0.673	0.82
50_S	258_R	0.673	0.82
26_N	233_N	0.672	0.82
90_F	99_A	0.668	0.82
26_N	50_S	0.667	0.82
243_A	247_T	0.663	0.81
95_K	99_A	0.662	0.81
10_L	133_S	0.662	0.81
140_T	150_A	0.661	0.81
268_I	272_V	0.659	0.81
89_S	93_C	0.659	0.81
43_S	283_N	0.657	0.81
143_G	148_V	0.655	0.81
52_G	258_R	0.648	0.80
220_K	278_E	0.647	0.80
276_M	283_N	0.647	0.80
32_Q	36_Q	0.647	0.80
6_M	9_M	0.647	0.80
241_A	248_L	0.646	0.80
247_T	250_A	0.646	0.80
96_A	99_A	0.645	0.80
9_M	12_T	0.643	0.79
172_C	207_V	0.642	0.79
29_E	240_S	0.642	0.79
142_G	211_G	0.641	0.79
113_F	138_L	0.639	0.79
240_S	261_L	0.638	0.79
31_T	66_M	0.637	0.79
94_A	122_K	0.636	0.79
39_E	42_K	0.634	0.79
25_L	63_V	0.634	0.79
248_L	260_Y	0.634	0.79
158_P	194_E	0.632	0.78
92_S	95_K	0.627	0.78
217_A	221_K	0.626	0.78
71_M	80_V	0.623	0.77
64_V	101_F	0.620	0.77
63_V	67_V	0.617	0.77
108_A	136_A	0.613	0.76
155_Y	191_G	0.612	0.76
92_S	100_G	0.611	0.76
6_M	10_L	0.609	0.76
88_S	92_S	0.607	0.75
46_I	81_A	0.605	0.75
124_V	169_G	0.605	0.75
23_F	276_M	0.604	0.75
219_I	232_V	0.602	0.75
93_C	123_V	0.598	0.74
6_M	46_I	0.596	0.74
136_A	168_T	0.596	0.74
5_S	102_T	0.596	0.74
144_Q	147_D	0.588	0.73
238_I	242_K	0.588	0.73
240_S	264_A	0.587	0.73
35_L	78_V	0.585	0.73
175_P	195_M	0.583	0.73
13_A	18_Y	0.582	0.72
69_A	73_E	0.581	0.72
119_T	123_V	0.581	0.72
61_K	69_A	0.580	0.72
165_V	170_I	0.576	0.72
35_L	47_L	0.574	0.71
271_T	275_K	0.572	0.71
90_F	97_I	0.570	0.71
14_K	203_G	0.567	0.70
262_G	266_E	0.567	0.70
190_L	222_S	0.566	0.70
117_V	163_E	0.566	0.70
18_Y	280_G	0.565	0.70
198_I	206_L	0.564	0.70
243_A	263_P	0.563	0.70
184_Y	226_G	0.561	0.70
90_F	123_V	0.561	0.70
232_V	235_E	0.560	0.70
116_N	139_G	0.560	0.70
28_L	62_T	0.558	0.69
43_S	276_M	0.556	0.69
15_E	197_E	0.555	0.69
212_T	235_E	0.553	0.69
211_G	232_V	0.551	0.68
68_K	71_M	0.551	0.68
248_L	252_P	0.551	0.68
159_K	163_E	0.550	0.68
259_K	263_P	0.550	0.68
133_S	172_C	0.548	0.68
199_G	203_G	0.547	0.68
81_A	102_T	0.547	0.68
170_I	204_L	0.544	0.67
219_I	278_E	0.542	0.67
59_G	95_K	0.541	0.67
177_L	225_L	0.541	0.67
91_E	100_G	0.538	0.66
160_E	167_R	0.537	0.66
140_T	194_E	0.536	0.66
42_K	283_N	0.536	0.66
150_A	154_I	0.535	0.66
186_G	234_T	0.535	0.66
180_V	188_P	0.534	0.66
82_I	102_T	0.534	0.66
63_V	70_L	0.533	0.66
182_G	188_P	0.533	0.66
33_A	37_A	0.532	0.66
247_T	263_P	0.531	0.65
257_P	260_Y	0.530	0.65
53_A	256_D	0.530	0.65
15_E	250_A	0.529	0.65
143_G	147_D	0.529	0.65
136_A	157_D	0.529	0.65
30_F	268_I	0.528	0.65
63_V	66_M	0.528	0.65
138_L	157_D	0.527	0.65
97_I	104_V	0.527	0.65
95_K	98_H	0.527	0.65
17_G	199_G	0.524	0.64
64_V	99_A	0.516	0.63
30_F	53_A	0.515	0.63
45_V	276_M	0.515	0.63
26_N	49_V	0.515	0.63
33_A	265_R	0.514	0.63
214_I	218_D	0.514	0.63
146_D	152_G	0.513	0.63
92_S	97_I	0.511	0.63
26_N	257_P	0.510	0.62
54_G	92_S	0.510	0.62
117_V	168_T	0.510	0.62
121_A	124_V	0.509	0.62
31_T	57_M	0.508	0.62
90_F	96_A	0.507	0.62
218_D	221_K	0.506	0.62
157_D	194_E	0.502	0.61
157_D	197_E	0.502	0.61
186_G	189_N	0.502	0.61
244_V	261_L	0.502	0.61
114_E	117_V	0.501	0.61
266_E	277_R	0.500	0.61

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4to8A	2	0.9754	100	0.04	Contact Map
1gvfA	3	0.9649	100	0.042	Contact Map
2iswA	2	0.9439	100	0.049	Contact Map
3n9rA	3	0.9614	100	0.054	Contact Map
1rvgA	3	0.9825	100	0.055	Contact Map
3elfA	4	0.9509	100	0.09	Contact Map
3pm6A	2	0.9614	100	0.105	Contact Map
1dosA	2	0.9404	100	0.113	Contact Map
3qm3A	4	0.9754	100	0.118	Contact Map
3txvA	2	0.8596	100	0.242	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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