GREMLIN Database
ALF - Probable fructose-bisphosphate aldolase
UniProt: P13243 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 285 (276)
Sequences: 3952 (2005)
Seq/√Len: 120.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
163_E167_R3.9761.00
40_E273_I3.3951.00
40_E269_K3.3391.00
12_T16_K3.0501.00
113_F163_E2.9971.00
41_E277_R2.7531.00
43_S281_S2.6841.00
113_F117_V2.5751.00
162_Q166_E2.5541.00
196_E200_K2.5091.00
97_I132_V2.4821.00
36_Q269_K2.4371.00
246_E250_A2.4111.00
39_E77_T2.3991.00
122_K125_E2.3931.00
112_P115_E2.3791.00
94_A126_L2.3641.00
191_G194_E2.3631.00
106_I119_T2.2661.00
216_T278_E2.2651.00
177_L195_M2.2521.00
34_I45_V2.2261.00
118_A122_K2.2061.00
49_V101_F2.1971.00
12_T18_Y2.1351.00
41_E273_I2.1311.00
6_M81_A2.0661.00
243_A267_A2.0321.00
128_H171_D1.9781.00
41_E282_S1.9721.00
98_H126_L1.9701.00
9_M81_A1.9511.00
196_E225_L1.8951.00
192_F221_K1.8841.00
121_A125_E1.8811.00
215_P218_D1.8721.00
266_E270_E1.8691.00
269_K273_I1.8551.00
11_N15_E1.8441.00
162_Q201_S1.8161.00
7_T11_N1.7901.00
277_R282_S1.7841.00
14_K228_A1.7581.00
221_K224_S1.7461.00
6_M172_C1.7261.00
115_E118_A1.7151.00
94_A98_H1.6721.00
35_L76_V1.6561.00
108_A111_H1.6451.00
22_Q48_G1.6321.00
124_V134_V1.6231.00
243_A246_E1.5771.00
160_E163_E1.5441.00
98_H130_H1.5311.00
159_K201_S1.5301.00
45_V80_V1.5211.00
192_F225_L1.5201.00
196_E221_K1.5111.00
276_M282_S1.5101.00
25_L47_L1.5051.00
114_E167_R1.5041.00
83_H103_S1.4861.00
111_H115_E1.4731.00
126_L130_H1.4661.00
206_L227_T1.4621.00
221_K225_L1.4551.00
14_K199_G1.4391.00
270_E273_I1.4271.00
242_K246_E1.4191.00
31_T70_L1.4151.00
35_L80_V1.4071.00
32_Q265_R1.4031.00
39_E76_V1.3541.00
68_K72_E1.3501.00
197_E200_K1.3491.00
21_G272_V1.3421.00
113_F160_E1.3251.00
157_D160_E1.3251.00
267_A270_E1.3111.00
216_T274_G1.2991.00
247_T251_K1.2671.00
179_S214_I1.2671.00
65_A68_K1.2550.99
245_R248_L1.2460.99
90_F94_A1.2440.99
31_T67_V1.2440.99
25_L31_T1.2400.99
224_S280_G1.2310.99
65_A69_A1.2310.99
271_T274_G1.2230.99
194_E198_I1.2190.99
90_F122_K1.2180.99
263_P266_E1.2110.99
113_F167_R1.1980.99
25_L34_I1.1930.99
51_E92_S1.1930.99
17_G224_S1.1890.99
37_A272_V1.1850.99
125_E129_F1.1780.99
42_K77_T1.1700.99
244_V264_A1.1700.99
223_I279_F1.1680.99
216_T220_K1.1670.99
62_T70_L1.1590.99
64_V82_I1.1580.99
37_A41_E1.1520.99
111_H119_T1.1490.99
120_T136_A1.1460.99
38_A45_V1.1400.99
68_K82_I1.1390.99
61_K72_E1.1230.99
123_V134_V1.1170.99
121_A167_R1.1120.99
5_S133_S1.1100.99
194_E197_E1.1100.99
114_E163_E1.1030.99
233_N237_Q1.0980.99
197_E201_S1.0870.98
119_T122_K1.0850.98
36_Q265_R1.0850.98
97_I100_G1.0840.98
61_K95_K1.0750.98
104_V134_V1.0730.98
270_E274_G1.0690.98
114_E118_A1.0680.98
104_V127_A1.0660.98
165_V204_L1.0630.98
104_V123_V1.0560.98
61_K73_E1.0500.98
117_V167_R1.0500.98
193_K197_E1.0440.98
94_A130_H1.0410.98
247_T254_E1.0410.98
37_A269_K1.0400.98
97_I130_H1.0330.98
97_I126_L1.0300.98
273_I277_R1.0220.98
69_A72_E1.0120.98
202_T206_L1.0120.98
175_P198_I1.0010.97
108_A119_T1.0000.97
21_G276_M0.9970.97
8_E12_T0.9970.97
12_T15_E0.9900.97
161_C202_T0.9890.97
91_E95_K0.9880.97
266_E269_K0.9870.97
158_P201_S0.9850.97
199_G228_A0.9850.97
49_V63_V0.9800.97
33_A268_I0.9760.97
193_K196_E0.9730.97
90_F93_C0.9640.97
26_N53_A0.9610.97
37_A273_I0.9580.97
21_G279_F0.9570.97
239_S271_T0.9560.97
251_K254_E0.9510.96
230_I279_F0.9490.96
76_V80_V0.9470.96
274_G278_E0.9440.96
9_M20_V0.9410.96
38_A276_M0.9380.96
150_A153_V0.9350.96
232_V279_F0.9340.96
191_G197_E0.9320.96
26_N30_F0.9160.96
10_L172_C0.9150.96
102_T133_S0.9150.96
136_A164_L0.9070.95
244_V260_Y0.9070.95
31_T47_L0.9010.95
97_I127_A0.9010.95
220_K224_S0.8920.95
115_E119_T0.8860.95
155_Y194_E0.8850.95
136_A170_I0.8840.95
93_C97_I0.8830.95
158_P197_E0.8820.95
17_G228_A0.8810.95
50_S53_A0.8700.94
71_M76_V0.8700.94
192_F218_D0.8670.94
144_Q149_I0.8650.94
34_I272_V0.8570.94
18_Y43_S0.8560.94
64_V68_K0.8520.94
43_S280_G0.8510.94
177_L208_L0.8500.94
272_V276_M0.8490.94
88_S110_H0.8490.94
208_L222_S0.8470.93
90_F100_G0.8460.93
96_A100_G0.8440.93
219_I279_F0.8430.93
67_V82_I0.8360.93
183_P238_I0.8340.93
219_I223_I0.8340.93
44_P79_P0.8270.93
5_S8_E0.8260.93
120_T124_V0.8230.92
10_L20_V0.8190.92
263_P267_A0.8150.92
92_S96_A0.8130.92
161_C198_I0.8120.92
126_L129_F0.8120.92
143_G146_D0.8100.92
93_C96_A0.8090.92
38_A78_V0.8080.92
254_E259_K0.8060.92
249_A252_P0.8050.92
51_E89_S0.8020.91
91_E94_A0.7960.91
37_A276_M0.7950.91
23_F45_V0.7940.91
23_F272_V0.7930.91
57_M63_V0.7910.91
27_N30_F0.7890.91
94_A97_I0.7880.91
51_E88_S0.7880.91
48_G233_N0.7870.91
179_S190_L0.7850.91
39_E78_V0.7820.90
156_A161_C0.7790.90
50_S233_N0.7760.90
61_K65_A0.7730.90
128_H131_G0.7730.90
276_M281_S0.7730.90
104_V132_V0.7730.90
219_I230_I0.7700.90
5_S131_G0.7690.90
215_P219_I0.7670.90
165_V202_T0.7670.90
48_G83_H0.7650.89
163_E166_E0.7610.89
145_E148_V0.7610.89
93_C100_G0.7610.89
127_A132_V0.7600.89
62_T66_M0.7590.89
220_K280_G0.7540.89
246_E249_A0.7540.89
159_K162_Q0.7530.89
90_F126_L0.7530.89
28_L66_M0.7520.89
49_V54_G0.7510.89
195_M227_T0.7500.88
235_E275_K0.7450.88
53_A233_N0.7450.88
138_L165_V0.7440.88
156_A175_P0.7440.88
168_T171_D0.7350.87
106_I123_V0.7340.87
123_V126_L0.7330.87
112_P118_A0.7300.87
87_G93_C0.7290.87
89_S92_S0.7290.87
147_D151_E0.7280.87
23_F34_I0.7280.87
198_I202_T0.7240.87
152_G157_D0.7230.87
24_N50_S0.7170.86
256_D259_K0.7170.86
195_M225_L0.7160.86
156_A198_I0.7150.86
184_Y188_P0.7130.86
6_M133_S0.7100.86
124_V128_H0.7100.86
8_E11_N0.7020.85
236_N268_I0.6930.84
138_L164_L0.6930.84
140_T153_V0.6900.84
11_N14_K0.6820.83
223_I226_G0.6820.83
106_I111_H0.6800.83
96_A101_F0.6790.83
243_A264_A0.6780.83
81_A133_S0.6780.83
217_A220_K0.6760.83
138_L161_C0.6750.83
245_R249_A0.6730.82
50_S258_R0.6730.82
26_N233_N0.6720.82
90_F99_A0.6680.82
26_N50_S0.6670.82
243_A247_T0.6630.81
95_K99_A0.6620.81
10_L133_S0.6620.81
140_T150_A0.6610.81
268_I272_V0.6590.81
89_S93_C0.6590.81
43_S283_N0.6570.81
143_G148_V0.6550.81
52_G258_R0.6480.80
220_K278_E0.6470.80
276_M283_N0.6470.80
32_Q36_Q0.6470.80
6_M9_M0.6470.80
241_A248_L0.6460.80
247_T250_A0.6460.80
96_A99_A0.6450.80
9_M12_T0.6430.79
172_C207_V0.6420.79
29_E240_S0.6420.79
142_G211_G0.6410.79
113_F138_L0.6390.79
240_S261_L0.6380.79
31_T66_M0.6370.79
94_A122_K0.6360.79
39_E42_K0.6340.79
25_L63_V0.6340.79
248_L260_Y0.6340.79
158_P194_E0.6320.78
92_S95_K0.6270.78
217_A221_K0.6260.78
71_M80_V0.6230.77
64_V101_F0.6200.77
63_V67_V0.6170.77
108_A136_A0.6130.76
155_Y191_G0.6120.76
92_S100_G0.6110.76
6_M10_L0.6090.76
88_S92_S0.6070.75
46_I81_A0.6050.75
124_V169_G0.6050.75
23_F276_M0.6040.75
219_I232_V0.6020.75
93_C123_V0.5980.74
6_M46_I0.5960.74
136_A168_T0.5960.74
5_S102_T0.5960.74
144_Q147_D0.5880.73
238_I242_K0.5880.73
240_S264_A0.5870.73
35_L78_V0.5850.73
175_P195_M0.5830.73
13_A18_Y0.5820.72
69_A73_E0.5810.72
119_T123_V0.5810.72
61_K69_A0.5800.72
165_V170_I0.5760.72
35_L47_L0.5740.71
271_T275_K0.5720.71
90_F97_I0.5700.71
14_K203_G0.5670.70
262_G266_E0.5670.70
190_L222_S0.5660.70
117_V163_E0.5660.70
18_Y280_G0.5650.70
198_I206_L0.5640.70
243_A263_P0.5630.70
184_Y226_G0.5610.70
90_F123_V0.5610.70
232_V235_E0.5600.70
116_N139_G0.5600.70
28_L62_T0.5580.69
43_S276_M0.5560.69
15_E197_E0.5550.69
212_T235_E0.5530.69
211_G232_V0.5510.68
68_K71_M0.5510.68
248_L252_P0.5510.68
159_K163_E0.5500.68
259_K263_P0.5500.68
133_S172_C0.5480.68
199_G203_G0.5470.68
81_A102_T0.5470.68
170_I204_L0.5440.67
219_I278_E0.5420.67
59_G95_K0.5410.67
177_L225_L0.5410.67
91_E100_G0.5380.66
160_E167_R0.5370.66
140_T194_E0.5360.66
42_K283_N0.5360.66
150_A154_I0.5350.66
186_G234_T0.5350.66
180_V188_P0.5340.66
82_I102_T0.5340.66
63_V70_L0.5330.66
182_G188_P0.5330.66
33_A37_A0.5320.66
247_T263_P0.5310.65
257_P260_Y0.5300.65
53_A256_D0.5300.65
15_E250_A0.5290.65
143_G147_D0.5290.65
136_A157_D0.5290.65
30_F268_I0.5280.65
63_V66_M0.5280.65
138_L157_D0.5270.65
97_I104_V0.5270.65
95_K98_H0.5270.65
17_G199_G0.5240.64
64_V99_A0.5160.63
30_F53_A0.5150.63
45_V276_M0.5150.63
26_N49_V0.5150.63
33_A265_R0.5140.63
214_I218_D0.5140.63
146_D152_G0.5130.63
92_S97_I0.5110.63
26_N257_P0.5100.62
54_G92_S0.5100.62
117_V168_T0.5100.62
121_A124_V0.5090.62
31_T57_M0.5080.62
90_F96_A0.5070.62
218_D221_K0.5060.62
157_D194_E0.5020.61
157_D197_E0.5020.61
186_G189_N0.5020.61
244_V261_L0.5020.61
114_E117_V0.5010.61
266_E277_R0.5000.61
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4to8A 2 0.9754 100 0.04 Contact Map
1gvfA 3 0.9649 100 0.042 Contact Map
2iswA 2 0.9439 100 0.049 Contact Map
3n9rA 3 0.9614 100 0.054 Contact Map
1rvgA 3 0.9825 100 0.055 Contact Map
3elfA 4 0.9509 100 0.09 Contact Map
3pm6A 2 0.9614 100 0.105 Contact Map
1dosA 2 0.9404 100 0.113 Contact Map
3qm3A 4 0.9754 100 0.118 Contact Map
3txvA 2 0.8596 100 0.242 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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