GREMLIN Database
ISP1 - Major intracellular serine protease
UniProt: P11018 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 319 (272)
Sequences: 15611 (11139)
Seq/√Len: 675.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
119_L126_G3.2251.00
40_K110_E3.1681.00
255_A301_L2.8471.00
203_G224_V2.8441.00
234_L240_G2.7691.00
255_A299_L2.5721.00
46_V95_I2.4541.00
204_S212_S2.4431.00
204_S214_F2.3971.00
280_L300_Y2.3081.00
90_H247_M2.2831.00
147_S249_A2.2141.00
42_K110_E2.2111.00
212_S228_E2.1501.00
214_F228_E2.1451.00
204_S227_G2.0591.00
90_H232_S2.0561.00
45_K112_S2.0481.00
116_V137_Y2.0161.00
267_F270_K2.0041.00
164_K198_E1.9861.00
94_T251_H1.9391.00
172_L200_I1.9331.00
93_G232_S1.9311.00
194_A201_A1.9191.00
45_K114_L1.9091.00
278_A304_P1.9031.00
93_G233_T1.8771.00
172_L256_L1.8681.00
48_V95_I1.8481.00
39_V261_S1.8461.00
144_D260_K1.8291.00
91_V250_P1.7881.00
259_I304_P1.7861.00
136_N165_N1.7821.00
234_L242_L1.7701.00
91_V147_S1.7281.00
302_T306_E1.7151.00
96_A113_L1.7071.00
260_K271_L1.7001.00
137_Y141_Q1.6761.00
46_V257_A1.6661.00
116_V134_G1.6441.00
157_P161_E1.6401.00
136_N140_E1.6371.00
273_E277_F1.6121.00
28_V209_R1.6071.00
107_V254_G1.6041.00
25_G251_H1.6011.00
83_D86_G1.5891.00
147_S253_S1.5821.00
256_L271_L1.5791.00
84_Y122_E1.5501.00
242_L247_M1.5431.00
174_V256_L1.5381.00
47_A138_A1.5321.00
203_G215_S1.5301.00
53_C115_I1.5301.00
249_A253_S1.5291.00
263_E266_S1.5181.00
139_V166_A1.5151.00
114_L143_V1.5051.00
252_V299_L1.4881.00
127_Q130_W1.4831.00
272_S276_V1.4721.00
174_V252_V1.4581.00
160_K164_K1.4581.00
44_I260_K1.4531.00
47_A143_V1.4431.00
266_S274_S1.4281.00
176_A249_A1.4241.00
205_V224_V1.4231.00
175_C199_V1.4231.00
174_V249_A1.4211.00
90_H242_L1.4181.00
145_I253_S1.4081.00
49_L118_V1.4071.00
203_G221_I1.4031.00
164_K168_K1.3731.00
204_S228_E1.3601.00
177_A201_A1.3541.00
205_V298_F1.3421.00
139_V171_V1.3241.00
46_V111_A1.3191.00
206_S228_E1.3131.00
30_K300_Y1.3111.00
40_K261_S1.3071.00
231_L241_K1.3031.00
97_A233_T1.2971.00
94_T247_M1.2941.00
300_Y303_A1.2891.00
48_V91_V1.2871.00
256_L275_E1.2851.00
209_R298_F1.2841.00
33_E302_T1.2601.00
167_V198_E1.2531.00
63_Q98_N1.2511.00
92_A115_I1.2501.00
228_E243_T1.2461.00
223_L275_E1.2371.00
92_A113_L1.2321.00
146_I171_V1.2301.00
181_G211_L1.2241.00
54_D236_N1.2171.00
54_D235_P1.2161.00
263_E274_S1.2151.00
165_N168_K1.1851.00
200_I272_S1.1811.00
135_I166_A1.1811.00
96_A111_A1.1671.00
217_A293_L1.1651.00
180_E216_N1.1601.00
259_I266_S1.1551.00
44_I257_A1.1541.00
108_A257_A1.1491.00
276_V296_N1.1411.00
30_K33_E1.1411.00
166_A173_V1.1361.00
131_I150_L1.1331.00
87_H246_S1.1291.00
69_N74_D1.1201.00
280_L298_F1.1071.00
132_I158_E1.0951.00
132_I162_A1.0921.00
225_A252_V1.0911.00
120_G123_N1.0911.00
223_L299_L1.0861.00
266_S270_K1.0781.00
148_M173_V1.0761.00
90_H94_T1.0741.00
211_L224_V1.0571.00
211_L215_S1.0571.00
24_E28_V1.0521.00
247_M250_P1.0381.00
135_I148_M1.0381.00
37_K302_T1.0331.00
65_I112_S1.0311.00
57_H239_Y1.0291.00
50_D87_H1.0271.00
26_I31_A1.0261.00
172_L271_L1.0231.00
62_N98_N1.0191.00
262_Y305_D1.0191.00
160_K195_A1.0131.00
165_N169_N1.0121.00
30_K302_T1.0091.00
148_M175_C1.0021.00
160_K196_Y1.0011.00
189_E220_E0.9971.00
291_K296_N0.9951.00
279_Q297_G0.9891.00
41_G108_A0.9801.00
177_A221_I0.9741.00
70_F137_Y0.9721.00
202_V252_V0.9711.00
43_N112_S0.9711.00
28_V205_V0.9681.00
170_G260_K0.9661.00
172_L268_Q0.9651.00
139_V169_N0.9631.00
274_S278_A0.9601.00
167_V272_S0.9531.00
48_V92_A0.9521.00
91_V247_M0.9441.00
282_R298_F0.9421.00
34_M305_D0.9401.00
108_A111_A0.9381.00
303_A306_E0.9371.00
33_E36_A0.9361.00
99_D105_A0.9181.00
31_A35_W0.9171.00
151_G176_A0.9161.00
281_I291_K0.9161.00
57_H233_T0.9151.00
179_N246_S0.9111.00
149_S250_P0.9111.00
58_P237_K0.9111.00
211_L217_A0.9071.00
172_L272_S0.9071.00
107_V251_H0.9051.00
118_V134_G0.9051.00
135_I146_I0.9051.00
126_G130_W0.9011.00
39_V262_Y0.9001.00
27_K32_P0.8941.00
290_A296_N0.8901.00
53_C92_A0.8881.00
202_V223_L0.8831.00
146_I173_V0.8771.00
118_V150_L0.8761.00
97_A104_I0.8701.00
223_L279_Q0.8701.00
69_N72_D0.8671.00
66_G115_I0.8651.00
204_S245_T0.8601.00
51_T85_N0.8581.00
163_V199_V0.8531.00
65_I114_L0.8521.00
70_F116_V0.8471.00
69_N117_K0.8451.00
23_P26_I0.8451.00
49_L134_G0.8431.00
164_K197_N0.8421.00
259_I271_L0.8411.00
45_K144_D0.8391.00
211_L293_L0.8331.00
137_Y140_E0.8301.00
59_D239_Y0.8291.00
35_W105_A0.8281.00
60_L64_I0.8271.00
32_P35_W0.8261.00
86_G242_L0.8251.00
281_I289_I0.8231.00
222_D276_V0.8211.00
192_Y196_Y0.8201.00
146_I166_A0.8181.00
206_S210_E0.8181.00
139_V165_N0.8141.00
87_H179_N0.8061.00
51_T83_D0.8061.00
226_P230_I0.8031.00
256_L259_I0.8021.00
49_L116_V0.8011.00
140_E165_N0.7991.00
57_H93_G0.7991.00
161_E165_N0.7981.00
205_V209_R0.7941.00
66_G114_L0.7901.00
145_I256_L0.7881.00
49_L148_M0.7851.00
29_I107_V0.7821.00
47_A114_L0.7801.00
52_G117_K0.7801.00
44_I261_S0.7781.00
283_R294_A0.7771.00
114_L141_Q0.7751.00
167_V269_R0.7751.00
192_Y195_A0.7751.00
55_T82_S0.7731.00
291_K294_A0.7721.00
53_C113_L0.7721.00
128_Y152_G0.7701.00
34_M37_K0.7691.00
260_K264_E0.7671.00
258_L301_L0.7631.00
232_S247_M0.7621.00
116_V141_Q0.7601.00
111_A257_A0.7501.00
260_K268_Q0.7481.00
128_Y159_L0.7471.00
45_K142_K0.7451.00
207_V229_N0.7431.00
172_L260_K0.7421.00
64_I115_I0.7391.00
116_V138_A0.7361.00
280_L303_A0.7321.00
226_P251_H0.7321.00
47_A116_V0.7291.00
130_W133_N0.7281.00
255_A275_E0.7251.00
164_K196_Y0.7241.00
37_K306_E0.7181.00
211_L294_A0.7151.00
59_D233_T0.7151.00
138_A146_I0.7111.00
129_E133_N0.7091.00
44_I144_D0.7081.00
223_L276_V0.7081.00
35_W109_P0.7071.00
53_C60_L0.7051.00
55_T64_I0.6981.00
95_I254_G0.6971.00
135_I163_V0.6961.00
83_D117_K0.6951.00
174_V253_S0.6931.00
61_K98_N0.6921.00
29_I251_H0.6921.00
214_F243_T0.6891.00
273_E276_V0.6891.00
229_N243_T0.6861.00
133_N136_N0.6861.00
246_S250_P0.6851.00
157_P160_K0.6851.00
32_P36_A0.6791.00
54_D58_P0.6741.00
180_E191_S0.6721.00
38_G41_G0.6721.00
170_G268_Q0.6721.00
177_A203_G0.6671.00
89_T233_T0.6671.00
138_A143_V0.6651.00
200_I276_V0.6651.00
162_A166_A0.6631.00
50_D88_G0.6631.00
167_V268_Q0.6621.00
161_E164_K0.6611.00
145_I172_L0.6611.00
268_Q272_S0.6581.00
176_A248_A0.6571.00
54_D237_K0.6571.00
47_A146_I0.6551.00
282_R294_A0.6551.00
68_K71_T0.6531.00
128_Y132_I0.6521.00
138_A166_A0.6511.00
145_I174_V0.6491.00
250_P253_S0.6481.00
200_I256_L0.6431.00
281_I284_T0.6431.00
57_H60_L0.6421.00
259_I263_E0.6361.00
39_V258_L0.6341.00
120_G125_S0.6321.00
108_A258_L0.6311.00
45_K143_V0.6301.00
274_S304_P0.6281.00
118_V131_I0.6251.00
196_Y199_V0.6231.00
59_D97_A0.6201.00
49_L146_I0.6171.00
149_S176_A0.6161.00
234_L238_K0.6151.00
222_D272_S0.6141.00
198_E269_R0.6141.00
200_I223_L0.6111.00
61_K64_I0.6091.00
224_V294_A0.6071.00
164_K167_V0.6061.00
40_K109_P0.6031.00
25_G28_V0.6011.00
168_K198_E0.6011.00
131_I148_M0.5991.00
94_T230_I0.5971.00
59_D104_I0.5941.00
174_V200_I0.5931.00
179_N250_P0.5881.00
136_N139_V0.5881.00
41_G261_S0.5881.00
224_V295_G0.5871.00
181_G217_A0.5871.00
182_D217_A0.5871.00
158_E161_E0.5821.00
97_A106_G0.5811.00
67_G115_I0.5811.00
24_E207_V0.5781.00
150_L175_C0.5781.00
270_K273_E0.5771.00
259_I305_D0.5741.00
91_V149_S0.5741.00
119_L150_L0.5711.00
132_I159_L0.5701.00
229_N241_K0.5681.00
24_E27_K0.5671.00
50_D246_S0.5661.00
255_A258_L0.5651.00
45_K65_I0.5621.00
41_G111_A0.5591.00
147_S250_P0.5581.00
95_I147_S0.5561.00
191_S194_A0.5561.00
160_K192_Y0.5561.00
23_P28_V0.5551.00
160_K198_E0.5551.00
155_D160_K0.5531.00
252_V275_E0.5521.00
86_G234_L0.5521.00
290_A293_L0.5511.00
200_I275_E0.5491.00
162_A165_N0.5461.00
251_H255_A0.5451.00
67_G70_F0.5421.00
56_S82_S0.5411.00
269_R273_E0.5401.00
218_N222_D0.5401.00
99_D109_P0.5391.00
221_I224_V0.5391.00
33_E37_K0.5341.00
204_S243_T0.5311.00
190_L220_E0.5311.00
274_S277_F0.5281.00
84_Y117_K0.5281.00
128_Y131_I0.5281.00
147_S174_V0.5271.00
29_I226_P0.5261.00
146_I150_L0.5261.00
167_V196_Y0.5241.00
182_D189_E0.5230.99
151_G180_E0.5220.99
44_I145_I0.5190.99
106_G254_G0.5170.99
173_V199_V0.5150.99
31_A107_V0.5150.99
63_Q112_S0.5150.99
126_G131_I0.5130.99
258_L261_S0.5120.99
201_A222_D0.5100.99
181_G216_N0.5100.99
145_I257_A0.5080.99
43_N261_S0.5080.99
27_K30_K0.5070.99
218_N221_I0.5070.99
85_N242_L0.5060.99
29_I301_L0.5060.99
209_R280_L0.5040.99
50_D179_N0.5030.99
60_L233_T0.5010.99
54_D234_L0.5000.99
69_N73_D0.5000.99
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4tr2A 2 0.9216 100 0.158 Contact Map
2x8jA 2 0.9373 100 0.158 Contact Map
1r6vA 1 0.9749 100 0.16 Contact Map
3whiA 2 0.9185 100 0.17 Contact Map
1to2E 1 0.8809 100 0.176 Contact Map
1r0rE 1 0.8589 100 0.176 Contact Map
1dbiA 1 0.8433 100 0.178 Contact Map
1gciA 1 0.8433 100 0.179 Contact Map
4lvnA 1 0.9122 100 0.179 Contact Map
2z30A 1 0.8934 100 0.184 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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