GREMLIN Database
BIRA - Bifunctional ligase/repressor BirA
UniProt: P0CI75 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 325 (278)
Sequences: 2781 (2158)
Seq/√Len: 129.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
298_L309_E3.9221.00
165_Q272_L3.8581.00
165_Q253_C3.4771.00
92_L98_T3.3621.00
91_V117_K3.0821.00
169_E253_C2.9911.00
98_T101_E2.8821.00
310_T315_K2.8381.00
176_D278_L2.8251.00
139_M247_I2.6551.00
99_A142_I2.6131.00
137_V247_I2.5781.00
168_E276_Y2.5561.00
140_S208_I2.5271.00
131_S138_W2.4601.00
89_H92_L2.4511.00
274_E299_G2.4351.00
76_G248_Q2.3921.00
301_D305_V2.3541.00
93_S97_K2.3021.00
116_D243_R2.2891.00
285_R294_Y2.2461.00
164_V276_Y2.2361.00
169_E249_H2.2201.00
114_V247_I2.2161.00
310_T313_G2.2061.00
168_E174_Q2.1801.00
165_Q257_R2.1231.00
112_L251_L2.1221.00
93_S98_T2.0591.00
185_I190_T2.0491.00
307_L314_I2.0381.00
163_V250_I2.0281.00
301_D316_K1.9941.00
283_N311_N1.9771.00
163_V191_V1.9211.00
283_N296_K1.8671.00
279_G297_A1.8601.00
270_K274_E1.8541.00
112_L141_L1.8521.00
99_A113_V1.8441.00
159_A209_I1.8391.00
155_L207_V1.8361.00
160_A177_I1.8151.00
162_A257_R1.7821.00
309_E314_I1.7271.00
231_T236_A1.7221.00
298_L314_I1.7021.00
93_S101_E1.7021.00
54_V57_K1.6661.00
286_A322_I1.6501.00
285_R292_T1.6381.00
283_N294_Y1.6321.00
280_I299_G1.5931.00
166_G250_I1.5901.00
89_H98_T1.5871.00
103_A142_I1.5531.00
286_A317_I1.5321.00
307_L316_K1.5211.00
102_L113_V1.5071.00
299_G307_L1.5021.00
185_I236_A1.4861.00
52_E60_R1.4701.00
183_I191_V1.4651.00
66_G242_D1.4641.00
52_E62_I1.4491.00
175_T183_I1.4451.00
109_E258_Y1.4241.00
131_S136_G1.4231.00
184_L278_L1.4061.00
231_T235_Q1.3961.00
296_K309_E1.3871.00
163_V183_I1.3781.00
162_A166_G1.3671.00
70_E74_R1.3601.00
224_D227_K1.3151.00
164_V175_T1.3121.00
187_G282_T1.3051.00
101_E105_N1.3001.00
211_I250_I1.2821.00
257_R272_L1.2501.00
96_Q208_I1.2421.00
166_G253_C1.2381.00
242_D245_G1.2321.00
79_T83_G1.2221.00
213_I250_I1.2211.00
136_G217_Q1.2191.00
167_I171_A1.2090.99
87_I102_L1.2010.99
78_K252_L1.1990.99
83_G255_E1.1970.99
129_W180_P1.1950.99
249_H252_L1.1890.99
246_V250_I1.1820.99
81_V259_R1.1720.99
186_N236_A1.1680.99
70_E88_Y1.1660.99
309_E313_G1.1640.99
215_V246_V1.1640.99
198_Q206_S1.1560.99
260_D264_H1.1540.99
68_L88_Y1.1400.99
73_I244_A1.1390.99
195_T207_V1.1390.99
98_T102_L1.1380.99
91_V119_T1.1360.99
159_A211_I1.1250.99
277_A280_I1.1200.99
254_F258_Y1.1150.99
305_V316_K1.1140.99
281_G298_L1.1130.99
286_A308_L1.1110.99
303_E316_K1.0970.99
287_R323_E1.0850.99
293_F315_K1.0850.99
284_M297_A1.0830.99
216_N234_S1.0830.99
157_L304_G1.0800.99
50_E63_K1.0780.99
218_Q234_S1.0720.99
163_V211_I1.0680.99
266_F270_K1.0630.99
250_I254_F1.0530.99
253_C256_K1.0500.99
274_E280_I1.0420.98
277_A297_A1.0420.98
298_L307_L1.0390.98
273_W277_A1.0360.98
87_I111_T1.0310.98
158_L257_R1.0260.98
168_E175_T1.0220.98
166_G170_A1.0200.98
107_A142_I1.0190.98
95_T115_A1.0180.98
80_E259_R1.0170.98
244_A248_Q1.0150.98
253_C257_R1.0130.98
175_T185_I1.0060.98
156_T195_T1.0030.98
191_V213_I1.0000.98
305_V318_Y0.9940.98
232_S235_Q0.9910.98
164_V183_I0.9880.98
108_P111_T0.9860.98
295_G310_T0.9830.98
271_L275_S0.9810.98
141_L254_F0.9750.98
69_S72_E0.9640.97
84_Q110_G0.9630.97
249_H253_C0.9580.97
164_V168_E0.9470.97
286_A293_F0.9450.97
83_G110_G0.9440.97
114_V139_M0.9420.97
79_T255_E0.9410.97
132_Q135_N0.9350.97
100_H208_I0.9350.97
110_G255_E0.9300.97
121_G131_S0.9280.97
103_A206_S0.9210.97
218_Q221_D0.9170.96
279_G322_I0.9150.96
132_Q138_W0.9140.96
190_T231_T0.9120.96
167_I175_T0.9100.96
139_M250_I0.9100.96
248_Q252_L0.9070.96
77_L248_Q0.9030.96
170_A249_H0.8970.96
161_V257_R0.8860.96
99_A140_S0.8740.95
127_R321_D0.8730.95
279_G299_G0.8720.95
161_V272_L0.8660.95
159_A254_F0.8650.95
209_I258_Y0.8590.95
72_E242_D0.8580.95
161_V269_I0.8580.95
266_F300_I0.8530.95
176_D187_G0.8460.95
161_V273_W0.8460.95
295_G308_L0.8440.94
89_H113_V0.8420.94
141_L251_L0.8420.94
96_Q320_A0.8360.94
270_K302_D0.8360.94
257_R260_D0.8340.94
152_T204_V0.8310.94
188_K231_T0.8300.94
87_I98_T0.8280.94
217_Q222_F0.8260.94
137_V215_V0.8260.94
272_L275_S0.8200.94
111_T142_I0.8190.94
102_L111_T0.8190.94
112_L139_M0.8180.93
271_L274_E0.8180.93
257_R269_I0.8170.93
45_R53_A0.8160.93
209_I254_F0.8090.93
73_I76_G0.8080.93
260_D272_L0.8080.93
215_V243_R0.8070.93
69_S244_A0.8050.93
269_I273_W0.8030.93
295_G317_I0.8030.93
158_L161_V0.8020.93
309_E312_E0.8000.93
143_L258_Y0.8000.93
68_L244_A0.7970.93
282_T297_A0.7960.93
90_D133_E0.7960.93
245_G248_Q0.7930.92
184_L279_G0.7910.92
154_Q261_Y0.7890.92
139_M211_I0.7890.92
267_T302_D0.7850.92
68_L73_I0.7830.92
293_F317_I0.7800.92
277_A299_G0.7780.92
50_E62_I0.7770.92
90_D116_D0.7760.92
108_P144_R0.7710.91
144_R206_S0.7690.91
166_G246_V0.7690.91
135_N221_D0.7620.91
234_S240_K0.7610.91
167_I213_I0.7600.91
166_G249_H0.7590.91
112_L247_I0.7580.91
308_L317_I0.7570.91
273_W300_I0.7510.90
245_G249_H0.7510.90
73_I247_I0.7510.90
68_L247_I0.7480.90
216_N232_S0.7420.90
135_N217_Q0.7380.89
272_L276_Y0.7370.89
147_I261_Y0.7340.89
161_V276_Y0.7330.89
116_D134_G0.7330.89
167_I246_V0.7330.89
98_T113_V0.7330.89
287_R290_N0.7310.89
222_F230_A0.7280.89
213_I246_V0.7220.88
88_Y244_A0.7210.88
195_T209_I0.7130.88
280_I297_A0.7120.88
97_K120_A0.7110.88
287_R292_T0.7090.87
237_A241_I0.7090.87
171_A246_V0.7080.87
117_K131_S0.7030.87
51_V60_R0.7030.87
184_L273_W0.7030.87
84_Q255_E0.7030.87
49_Y65_P0.7000.87
45_R50_E0.7000.87
167_I233_L0.6990.87
247_I251_L0.6970.87
252_L256_K0.6970.87
159_A195_T0.6940.86
79_T84_Q0.6900.86
115_A138_W0.6850.86
286_A320_A0.6850.86
87_I92_L0.6840.86
82_M255_E0.6820.85
174_Q186_N0.6810.85
125_M323_E0.6810.85
293_F310_T0.6800.85
165_Q276_Y0.6780.85
68_L243_R0.6780.85
73_I86_L0.6780.85
61_L64_K0.6720.85
172_G175_T0.6700.84
99_A111_T0.6660.84
216_N238_G0.6640.84
58_G200_E0.6640.84
129_W157_L0.6610.84
234_S237_A0.6570.83
226_L229_I0.6570.83
288_T293_F0.6550.83
72_E244_A0.6530.83
139_M251_L0.6510.83
218_Q232_S0.6480.82
163_V213_I0.6480.82
130_H223_P0.6470.82
279_G282_T0.6460.82
145_P258_Y0.6430.82
295_G304_G0.6420.82
132_Q217_Q0.6410.82
161_V165_Q0.6380.81
164_V177_I0.6380.81
226_L230_A0.6370.81
197_M207_V0.6330.81
305_V321_D0.6320.81
217_Q230_A0.6310.81
73_I248_Q0.6310.81
157_L160_A0.6300.81
117_K133_E0.6290.80
219_P235_Q0.6270.80
155_L195_T0.6260.80
81_V109_E0.6250.80
143_L209_I0.6240.80
59_Y200_E0.6240.80
149_L197_M0.6220.80
109_E143_L0.6220.80
216_N239_E0.6220.80
158_L207_V0.6200.79
109_E144_R0.6190.79
96_Q140_S0.6180.79
110_G142_I0.6180.79
129_W304_G0.6170.79
197_M273_W0.6150.79
167_I183_I0.6140.79
77_L86_L0.6140.79
152_T197_M0.6140.79
48_G58_G0.6120.79
138_W217_Q0.6110.78
76_G245_G0.6090.78
117_K130_H0.6080.78
51_V58_G0.6070.78
85_H111_T0.6050.78
256_K260_D0.6040.78
148_P151_K0.6000.77
53_A60_R0.5990.77
265_G268_P0.5990.77
167_I190_T0.5980.77
185_I231_T0.5970.77
97_K101_E0.5950.77
293_F308_L0.5940.77
294_Y311_N0.5940.77
136_G183_I0.5900.76
154_Q266_F0.5890.76
145_P204_V0.5890.76
160_A193_I0.5890.76
72_E76_G0.5860.76
217_Q232_S0.5850.75
153_P157_L0.5830.75
86_L112_L0.5790.75
97_K122_R0.5780.75
259_R262_M0.5780.75
154_Q158_L0.5760.74
151_K154_Q0.5760.74
129_W216_N0.5760.74
49_Y61_L0.5750.74
217_Q235_Q0.5740.74
286_A295_G0.5730.74
99_A102_L0.5700.74
149_L152_T0.5700.74
49_Y52_E0.5700.74
70_E73_I0.5690.73
215_V241_I0.5690.73
260_D268_P0.5680.73
297_A306_L0.5680.73
92_L115_A0.5670.73
129_W197_M0.5670.73
107_A111_T0.5670.73
187_G278_L0.5640.73
188_K236_A0.5640.73
251_L255_E0.5640.73
102_L105_N0.5600.72
180_P273_W0.5590.72
167_I170_A0.5580.72
45_R52_E0.5580.72
274_E277_A0.5570.72
157_L193_I0.5540.71
126_S321_D0.5520.71
241_I246_V0.5520.71
297_A308_L0.5520.71
158_L261_Y0.5500.71
48_G59_Y0.5470.71
156_T177_I0.5460.70
273_W279_G0.5460.70
169_E245_G0.5420.70
230_A235_Q0.5390.69
274_E307_L0.5380.69
71_S75_F0.5360.69
106_N109_E0.5360.69
107_A110_G0.5360.69
100_H198_Q0.5340.69
173_I186_N0.5330.69
46_K281_G0.5320.68
51_V59_Y0.5280.68
89_H93_S0.5260.68
268_P271_L0.5230.67
49_Y72_E0.5220.67
157_L180_P0.5220.67
56_R59_Y0.5220.67
102_L106_N0.5220.67
180_P304_G0.5210.67
92_L113_V0.5200.67
266_F274_E0.5180.66
284_M317_I0.5170.66
217_Q221_D0.5150.66
96_Q194_L0.5140.66
255_E258_Y0.5130.66
168_E275_S0.5130.66
141_L255_E0.5130.66
103_A106_N0.5130.66
142_I206_S0.5120.66
143_L254_F0.5120.66
171_A233_L0.5120.66
200_E203_R0.5120.66
288_T291_G0.5110.65
64_K120_A0.5090.65
154_Q265_G0.5070.65
279_G284_M0.5070.65
90_D101_E0.5050.64
102_L107_A0.5040.64
257_R274_E0.5040.64
72_E75_F0.5040.64
149_L201_E0.5020.64
87_I113_V0.5020.64
96_Q196_E0.5020.64
110_G258_Y0.5000.64
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3rkxA 1 0.9446 100 0.163 Contact Map
1biaA 1 0.8954 100 0.191 Contact Map
4op0A 1 0.7815 100 0.34 Contact Map
2eayA 2 0.6738 100 0.343 Contact Map
2dxuA 2 0.7015 100 0.349 Contact Map
2ej9A 1 0.7046 100 0.368 Contact Map
3bfmA 2 0.6954 100 0.474 Contact Map
1vqzA 1 0.7323 99.9 0.741 Contact Map
2p5iA 1 0.6738 99.9 0.772 Contact Map
2qhsA 1 0.6031 99.7 0.819 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0097 seconds.