GREMLIN Database
NADE - NH(3)-dependent NAD(+) synthetase
UniProt: P08164 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 272 (238)
Sequences: 4538 (2531)
Seq/√Len: 164.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
201_E205_L4.8871.00
78_V147_I3.6641.00
59_A156_L3.4671.00
41_G153_L3.4321.00
251_K255_M3.1571.00
23_I196_E2.8421.00
93_L202_R2.7461.00
85_Q89_D2.6221.00
77_A100_P2.4961.00
248_A252_R2.4451.00
54_L194_L2.4031.00
34_V42_F2.2781.00
20_K196_E2.2761.00
30_L58_L2.1901.00
243_A247_E2.1431.00
46_I95_L2.0751.00
90_D206_K2.0701.00
257_E261_Q2.0661.00
109_K113_S2.0451.00
95_L103_S2.0351.00
56_G100_P1.9621.00
92_Q96_K1.9141.00
45_G144_Q1.8861.00
19_P192_T1.8751.00
115_F142_I1.8681.00
55_A182_L1.8471.00
139_T142_I1.8181.00
231_E245_V1.7721.00
23_I61_L1.7441.00
110_S117_D1.7201.00
50_Q194_L1.6571.00
42_F63_V1.6521.00
90_D202_R1.6171.00
79_R103_S1.6051.00
195_K201_E1.6031.00
93_L96_K1.5981.00
115_F139_T1.5911.00
238_G241_V1.5831.00
76_I104_W1.5481.00
192_T195_K1.5411.00
92_Q105_K1.5371.00
183_T266_M1.5021.00
171_K258_H1.4991.00
191_R230_D1.4991.00
220_S223_T1.4961.00
228_S231_E1.4881.00
53_T203_L1.4841.00
231_E242_S1.4771.00
28_N32_Q1.4761.00
20_K24_E1.4481.00
135_V142_I1.4461.00
169_F175_G1.4431.00
76_I153_L1.4411.00
186_T189_Q1.4321.00
147_I151_E1.4251.00
41_G76_I1.4131.00
58_L181_P1.3961.00
195_K204_Y1.3921.00
222_E228_S1.3871.00
131_N146_A1.3771.00
207_E220_S1.3731.00
252_R255_M1.3701.00
112_V139_T1.3561.00
166_T232_I1.3481.00
42_F59_A1.3401.00
94_A203_L1.3261.00
93_L97_F1.3201.00
41_G74_Q1.3181.00
40_K152_G1.3141.00
42_F156_L1.3121.00
188_R234_D1.3111.00
161_A184_G1.3091.00
37_T154_L1.3061.00
166_T225_L1.3031.00
64_E72_D1.3021.00
60_Q72_D1.2941.00
177_A180_L1.2931.00
79_R88_E1.2921.00
234_D239_K1.2851.00
111_T118_Q1.2801.00
86_Q202_R1.2761.00
24_E28_N1.2581.00
89_D202_R1.2511.00
50_Q190_G1.2421.00
30_L42_F1.2411.00
236_L253_Y1.2381.00
56_G98_I1.2371.00
61_L197_L1.2351.00
56_G77_A1.2341.00
57_R61_L1.2331.00
92_Q103_S1.2321.00
252_R256_T1.2261.00
112_V116_S1.2141.00
33_Y36_K1.2101.00
256_T260_R1.1921.00
186_T236_L1.1891.00
43_V147_I1.1801.00
248_A251_K1.1781.00
88_E92_Q1.1701.00
89_D93_L1.1691.00
60_Q98_I1.1631.00
23_I197_L1.1551.00
195_K205_L1.1541.00
64_E75_F1.1471.00
88_E105_K1.1451.00
111_T139_T1.1431.00
160_H221_D1.1351.00
79_R91_A1.1331.00
166_T252_R1.1141.00
34_V63_V1.0881.00
31_K35_K1.0871.00
175_G263_P1.0791.00
60_Q75_F1.0760.99
111_T143_A1.0730.99
55_A156_L1.0690.99
104_W151_E1.0670.99
185_L189_Q1.0650.99
77_A95_L1.0610.99
244_K248_A1.0590.99
50_Q204_Y1.0490.99
101_D104_W1.0450.99
23_I27_V1.0430.99
234_D241_V1.0380.99
247_E251_K1.0300.99
87_D211_D1.0300.99
27_V31_K1.0260.99
86_Q206_K1.0190.99
213_L224_E1.0170.99
111_T115_F1.0160.99
172_Y176_G1.0150.99
156_L179_L1.0050.99
27_V62_A0.9990.99
23_I58_L0.9970.99
237_E240_E0.9900.99
57_R98_I0.9850.99
191_R207_E0.9820.99
79_R92_Q0.9780.99
34_V37_T0.9770.99
159_D169_F0.9770.99
210_A219_Q0.9740.99
30_L179_L0.9700.99
257_E260_R0.9660.99
157_G177_A0.9640.99
113_S116_S0.9620.99
111_T114_A0.9600.99
210_A221_D0.9570.99
26_R58_L0.9500.99
113_S117_D0.9230.98
119_Y146_A0.9140.98
189_Q192_T0.9140.98
97_F199_A0.9130.98
92_Q95_L0.9040.98
208_P221_D0.8970.98
180_L183_T0.8900.98
141_M176_G0.8890.98
60_Q64_E0.8820.98
179_L267_F0.8810.98
77_A103_S0.8780.98
53_T94_A0.8780.98
32_Q36_K0.8730.98
114_A117_D0.8730.98
235_Y250_E0.8710.98
178_D258_H0.8710.98
83_G86_Q0.8700.98
171_K178_D0.8680.98
108_I139_T0.8660.98
80_L143_A0.8660.98
242_S245_V0.8650.98
106_F143_A0.8610.98
44_L55_A0.8610.98
106_F118_Q0.8560.97
41_G151_E0.8540.97
89_D92_Q0.8510.97
246_S250_E0.8490.97
35_K74_Q0.8450.97
219_Q224_E0.8420.97
155_V176_G0.8380.97
139_T143_A0.8380.97
164_A169_F0.8290.97
22_E189_Q0.8160.97
30_L181_P0.8160.97
110_S118_Q0.8150.97
233_D236_L0.8120.96
132_K150_Q0.8050.96
43_V153_L0.8010.96
100_P103_S0.8000.96
194_L204_Y0.7950.96
145_Y178_D0.7940.96
107_D110_S0.7920.96
125_D150_Q0.7910.96
134_N170_T0.7900.96
168_F224_E0.7890.96
131_N150_Q0.7880.96
188_R237_E0.7870.96
168_F210_A0.7860.96
114_A118_Q0.7850.96
235_Y246_S0.7800.96
39_A152_G0.7780.96
244_K247_E0.7730.95
160_H208_P0.7690.95
155_V258_H0.7660.95
45_G80_L0.7610.95
63_V75_F0.7580.95
27_V61_L0.7530.95
40_K74_Q0.7510.95
28_N31_K0.7460.94
34_V41_G0.7440.94
61_L64_E0.7410.94
93_L200_P0.7310.94
63_V74_Q0.7260.94
76_I151_E0.7260.94
26_R184_G0.7250.94
59_A75_F0.7220.93
172_Y177_A0.7220.93
94_A97_F0.7220.93
232_I249_L0.7170.93
190_G194_L0.7110.93
110_S114_A0.7100.93
183_T267_F0.7060.93
148_G258_H0.7030.92
133_G224_E0.7010.92
123_T150_Q0.6980.92
44_L56_G0.6970.92
19_P196_E0.6950.92
146_A150_Q0.6940.92
79_R105_K0.6940.92
30_L63_V0.6930.92
135_V146_A0.6920.92
119_Y123_T0.6910.92
22_E26_R0.6910.92
164_A183_T0.6880.92
108_I112_V0.6870.92
110_S113_S0.6830.91
60_Q100_P0.6820.91
80_L144_Q0.6790.91
168_F219_Q0.6770.91
164_A263_P0.6760.91
44_L75_F0.6730.91
188_R233_D0.6730.91
77_A203_L0.6720.91
219_Q223_T0.6700.91
72_D99_K0.6690.90
136_K211_D0.6670.90
157_G176_G0.6660.90
82_H113_S0.6650.90
89_D96_K0.6600.90
80_L155_V0.6570.90
226_G229_Y0.6560.90
54_L190_G0.6560.90
254_S257_E0.6530.89
220_S224_E0.6500.89
192_T196_E0.6470.89
207_E222_E0.6460.89
83_G109_K0.6450.89
76_I101_D0.6440.89
134_N138_R0.6420.89
191_R204_Y0.6410.89
250_E254_S0.6400.88
101_D153_L0.6350.88
86_Q90_D0.6330.88
160_H210_A0.6310.88
24_E27_V0.6280.88
87_D206_K0.6270.87
31_K62_A0.6260.87
243_A246_S0.6260.87
57_R94_A0.6250.87
78_V143_A0.6230.87
188_R230_D0.6200.87
231_E234_D0.6200.87
157_G181_P0.6190.87
135_V139_T0.6190.87
164_A256_T0.6190.87
42_F154_L0.6180.87
133_G213_L0.6180.87
131_N149_G0.6170.87
57_R97_F0.6150.86
232_I235_Y0.6130.86
22_E25_D0.6130.86
54_L197_L0.6130.86
57_R64_E0.6110.86
237_E241_V0.6110.86
106_F119_Y0.6090.86
245_V248_A0.6050.86
121_Q124_G0.5980.85
47_S51_D0.5980.85
162_A232_I0.5950.85
46_I94_A0.5950.85
179_L183_T0.5930.84
88_E91_A0.5910.84
128_T131_N0.5910.84
133_G137_A0.5890.84
165_V253_Y0.5890.84
191_R195_K0.5880.84
161_A183_T0.5870.84
21_Q25_D0.5860.84
104_W107_D0.5850.84
57_R199_A0.5820.83
231_E235_Y0.5810.83
32_Q35_K0.5790.83
104_W147_I0.5770.83
130_F133_G0.5770.83
95_L100_P0.5770.83
128_T150_Q0.5770.83
116_S135_V0.5690.82
129_D132_K0.5680.82
159_D175_G0.5600.81
164_A180_L0.5590.81
159_D164_A0.5590.81
85_Q88_E0.5570.81
222_E230_D0.5560.80
84_T211_D0.5560.80
86_Q89_D0.5560.80
18_D21_Q0.5550.80
112_V135_V0.5510.80
37_T183_T0.5500.80
79_R83_G0.5500.80
214_D218_Q0.5460.79
42_F75_F0.5440.79
78_V104_W0.5440.79
182_L190_G0.5390.78
241_V246_S0.5380.78
159_D180_L0.5370.78
46_I53_T0.5370.78
137_A212_L0.5330.78
222_E234_D0.5330.78
133_G136_K0.5330.78
36_K257_E0.5320.77
84_T206_K0.5300.77
90_D93_L0.5290.77
50_Q191_R0.5290.77
50_Q187_K0.5270.77
87_D136_K0.5240.76
42_F179_L0.5230.76
109_K112_V0.5230.76
56_G94_A0.5200.76
148_G153_L0.5180.76
126_Q129_D0.5170.75
30_L156_L0.5150.75
47_S163_E0.5150.75
159_D177_A0.5150.75
134_N137_A0.5130.75
43_V78_V0.5130.75
141_M145_Y0.5120.75
176_G180_L0.5100.74
238_G242_S0.5080.74
60_Q77_A0.5080.74
64_E99_K0.5080.74
86_Q91_A0.5070.74
183_T186_T0.5070.74
30_L153_L0.5060.74
50_Q54_L0.5060.74
79_R95_L0.5050.74
175_G178_D0.5040.74
240_E246_S0.5030.73
166_T249_L0.5010.73
171_K175_G0.5010.73
43_V148_G0.5000.73
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1kqpA 2 0.9926 100 0.326 Contact Map
1wxiA 2 0.9412 100 0.328 Contact Map
4q16A 2 0.9963 100 0.334 Contact Map
4xfdA 1 0.9081 100 0.345 Contact Map
3q4gA 2 0.9338 100 0.345 Contact Map
3ilvA 2 0.8713 100 0.362 Contact Map
3dpiA 3 0.9449 100 0.363 Contact Map
4f4hA 2 0.8971 100 0.388 Contact Map
3n05A 2 0.875 100 0.402 Contact Map
1xngA 2 0.875 100 0.403 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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