GREMLIN Database
SP2D - Stage II sporulation protein D
UniProt: P07372 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 343 (270)
Sequences: 1480 (1221)
Seq/√Len: 74.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
96_E99_K4.4671.00
128_D133_F4.2361.00
168_I175_P3.7311.00
103_L316_V3.5621.00
167_K316_V3.4611.00
169_L320_V3.3831.00
184_S302_S3.2271.00
192_E198_A3.1441.00
159_D163_S3.0551.00
107_T175_P3.0171.00
128_D132_M2.9811.00
307_N311_K2.9171.00
169_L327_T2.7761.00
246_G255_T2.7131.00
82_I158_T2.6881.00
82_I162_A2.6421.00
57_A153_K2.5181.00
94_K307_N2.4861.00
218_A292_T2.3601.00
170_T330_S2.3231.00
103_L320_V2.2921.00
171_Y200_P2.2311.00
210_D302_S2.1641.00
187_Y204_S2.1411.00
95_P163_S2.1161.00
89_M98_L2.1041.00
315_T318_D2.0211.00
252_Q298_G2.0211.00
189_E193_A1.9981.00
305_G323_Y1.9691.00
99_K163_S1.9291.00
76_P79_E1.9101.00
207_S302_S1.8841.00
221_T289_T1.8681.00
184_S210_D1.8401.00
314_K318_D1.8331.00
79_E82_I1.8231.00
321_K327_T1.7981.00
104_A324_Y1.7831.00
167_K317_D1.7831.00
95_P159_D1.7661.00
281_W290_V1.7431.00
169_L329_I1.7011.00
93_F304_Y1.6941.00
243_K257_V1.6851.00
223_T226_E1.6551.00
78_E165_Q1.6321.00
87_S137_K1.6221.00
106_R164_T1.6090.99
190_N193_A1.5900.99
104_A301_M1.5880.99
187_Y206_K1.5720.99
192_E203_K1.5610.99
191_A203_K1.5230.99
94_K311_K1.5120.99
86_A158_T1.5050.99
62_I69_N1.5040.99
245_T257_V1.4990.99
190_N203_K1.4960.99
221_T287_T1.4900.99
78_E166_G1.4850.99
246_G254_A1.4790.99
107_T176_I1.4670.99
248_T254_A1.4580.99
61_S74_N1.4320.99
179_S195_W1.4270.99
282_K291_T1.4100.99
239_S258_I1.4090.99
162_A165_Q1.4010.99
317_D321_K1.3960.99
219_T291_T1.3960.99
181_F188_T1.3940.99
255_T262_T1.3940.99
188_T205_V1.3900.98
253_V256_A1.3840.98
109_I127_V1.3620.98
275_N295_F1.3250.98
189_E248_T1.3240.98
215_K275_N1.3240.98
321_K328_Q1.3190.98
95_P156_K1.2900.98
75_I136_Y1.2790.97
309_M314_K1.2750.97
99_K316_V1.2570.97
181_F185_N1.2570.97
253_V279_F1.2530.97
167_K331_E1.2380.97
198_A203_K1.2200.97
81_V161_V1.2110.96
173_N330_S1.2010.96
263_L268_I1.1960.96
252_Q278_D1.1940.96
217_K293_R1.1870.96
252_Q277_A1.1860.96
86_A157_I1.1770.96
80_Y134_Q1.1670.95
111_R177_E1.1660.95
181_F184_S1.1480.95
171_Y201_Y1.1430.95
170_T328_Q1.1400.95
169_L178_A1.1390.95
185_N278_D1.1320.95
100_A306_A1.1300.95
96_E316_V1.1140.94
94_K310_A1.1100.94
183_T213_S1.1070.94
184_S207_S1.1040.94
283_R288_I1.1020.94
83_G87_S1.0950.94
209_W309_M1.0920.94
110_V175_P1.0900.94
107_T111_R1.0840.93
237_G260_G1.0830.93
308_F311_K1.0800.93
185_N252_Q1.0780.93
108_F182_S1.0770.93
207_S210_D1.0720.93
183_T216_Y1.0550.92
103_L167_K1.0510.92
190_N204_S1.0480.92
167_K327_T1.0440.92
250_G298_G1.0420.92
205_V322_Y1.0400.92
281_W288_I1.0260.91
318_D321_K1.0170.91
116_N133_F1.0150.91
78_E106_R1.0100.91
309_M312_E1.0090.91
89_M97_A0.9950.90
228_Q238_S0.9860.90
207_S323_Y0.9790.89
193_A249_P0.9770.89
98_L160_A0.9640.88
95_P160_A0.9590.88
242_G258_I0.9560.88
264_K267_D0.9560.88
226_E229_Q0.9550.88
183_T210_D0.9460.88
89_M93_F0.9380.87
80_Y84_V0.9360.87
85_V90_P0.9330.87
189_E252_Q0.9300.87
209_W314_K0.9270.86
90_P93_F0.9230.86
167_K329_I0.9220.86
321_K329_I0.9180.86
284_N289_T0.9160.86
169_L176_I0.9100.85
241_V283_R0.9070.85
220_K275_N0.9060.85
73_E136_Y0.9060.85
116_N128_D0.9040.85
192_E196_T0.9040.85
308_F312_E0.9010.85
297_H303_Q0.8940.84
72_V126_L0.8900.84
98_L157_I0.8850.84
77_L110_V0.8850.84
87_S135_V0.8840.84
158_T162_A0.8810.84
170_T173_N0.8800.83
245_T253_V0.8770.83
105_A134_Q0.8760.83
65_Y70_Q0.8760.83
103_L317_D0.8760.83
306_A310_A0.8750.83
111_R174_Q0.8730.83
257_V262_T0.8690.83
155_K159_D0.8610.82
175_P178_A0.8590.82
191_A199_I0.8580.82
69_N73_E0.8530.81
207_S322_Y0.8500.81
219_T289_T0.8490.81
309_M322_Y0.8460.81
122_P130_T0.8460.81
84_V324_Y0.8450.81
227_F288_I0.8440.81
75_I286_D0.8420.81
111_R175_P0.8390.80
227_F230_K0.8390.80
95_P99_K0.8380.80
240_A261_K0.8360.80
85_V157_I0.8340.80
101_Q105_A0.8330.80
181_F322_Y0.8250.79
200_P326_G0.8250.79
266_R270_E0.8230.79
241_V259_N0.8190.79
113_M180_F0.8190.79
243_K259_N0.8160.79
112_L115_S0.8150.78
101_Q303_Q0.8130.78
60_V71_S0.8100.78
244_I253_V0.8090.78
250_G277_A0.8090.78
231_L258_I0.8080.78
105_A180_F0.8060.78
64_V68_A0.8040.78
57_A79_E0.8030.77
178_A327_T0.8030.77
49_S53_E0.8030.77
73_E138_S0.8020.77
84_V164_T0.7980.77
280_E293_R0.7970.77
89_M304_Y0.7910.76
253_V265_G0.7880.76
227_F290_V0.7860.76
193_A254_A0.7830.76
73_E292_T0.7830.76
131_Q295_F0.7820.76
228_Q239_S0.7730.75
88_E269_R0.7720.75
105_A272_L0.7690.74
224_A288_I0.7680.74
66_R73_E0.7680.74
268_I272_L0.7610.74
309_M318_D0.7550.73
279_F290_V0.7550.73
224_A228_Q0.7540.73
88_E297_H0.7510.73
225_A318_D0.7490.72
85_V105_A0.7470.72
321_K325_Q0.7470.72
229_Q233_V0.7430.72
176_I324_Y0.7420.72
102_A161_V0.7340.71
88_E303_Q0.7310.71
104_A320_V0.7310.71
51_G54_T0.7310.71
320_V327_T0.7300.70
84_V106_R0.7280.70
189_E250_G0.7230.70
230_K271_K0.7220.70
80_Y109_I0.7220.70
170_T176_I0.7210.69
274_L322_Y0.7070.68
90_P307_N0.7060.68
100_A310_A0.7050.68
153_K158_T0.7040.68
125_S129_D0.7040.68
228_Q232_G0.7040.68
321_K326_G0.6980.67
84_V88_E0.6940.67
205_V325_Q0.6920.66
184_S188_T0.6860.66
69_N282_K0.6830.65
248_T265_G0.6810.65
69_N138_S0.6740.64
83_G139_K0.6730.64
116_N132_M0.6720.64
184_S248_T0.6710.64
183_T215_K0.6680.64
278_D293_R0.6670.63
171_Y326_G0.6640.63
266_R271_K0.6630.63
183_T214_P0.6610.63
88_E134_Q0.6600.63
222_F272_L0.6570.62
182_S213_S0.6570.62
225_A229_Q0.6530.62
245_T255_T0.6500.61
176_I201_Y0.6480.61
107_T178_A0.6450.61
194_Y266_R0.6430.61
49_S52_A0.6420.61
135_V182_S0.6410.60
190_N198_A0.6410.60
171_Y202_L0.6410.60
209_W308_F0.6410.60
130_T135_V0.6400.60
258_I263_L0.6390.60
276_S294_G0.6390.60
82_I153_K0.6380.60
237_G261_K0.6370.60
247_E280_E0.6350.60
322_Y325_Q0.6320.59
238_S261_K0.6320.59
81_V292_T0.6320.59
51_G55_L0.6310.59
93_F307_N0.6300.59
191_A198_A0.6300.59
84_V105_A0.6290.59
98_L275_N0.6280.59
222_F290_V0.6270.59
58_S136_Y0.6260.59
179_S202_L0.6220.58
56_K292_T0.6190.58
62_I71_S0.6190.58
156_K159_D0.6180.58
57_A82_I0.6160.57
183_T268_I0.6160.57
58_S73_E0.6130.57
84_V127_V0.6130.57
228_Q231_L0.6120.57
125_S128_D0.6120.57
213_S216_Y0.6110.57
239_S283_R0.6080.56
108_F179_S0.6080.56
114_V175_P0.6070.56
102_A160_A0.6070.56
81_V109_I0.6070.56
182_S295_F0.6070.56
317_D329_I0.6050.56
112_L127_V0.6040.56
113_M127_V0.6030.56
110_V168_I0.6000.55
220_K290_V0.5970.55
78_E164_T0.5970.55
289_T301_M0.5950.55
188_T204_S0.5920.54
169_L321_K0.5920.54
136_Y181_F0.5920.54
225_A243_K0.5910.54
77_L109_I0.5900.54
131_Q230_K0.5900.54
155_K158_T0.5880.54
59_P258_I0.5840.53
218_A239_S0.5830.53
90_P184_S0.5820.53
229_Q240_A0.5810.53
314_K322_Y0.5790.53
182_S304_Y0.5780.53
217_K247_E0.5760.52
185_N292_T0.5740.52
268_I279_F0.5730.52
168_I329_I0.5730.52
228_Q242_G0.5730.52
58_S138_S0.5720.52
87_S131_Q0.5710.52
224_A283_R0.5660.51
213_S304_Y0.5660.51
112_L133_F0.5650.51
239_S242_G0.5640.51
111_R114_V0.5640.51
220_K273_G0.5640.51
82_I108_F0.5600.50
199_I202_L0.5590.50
92_T98_L0.5590.50
268_I274_L0.5580.50
224_A239_S0.5540.50
325_Q331_E0.5540.50
116_N124_G0.5530.50
284_N287_T0.5530.50
81_V260_G0.5530.50
281_W313_G0.5530.50
56_K73_E0.5520.49
256_A272_L0.5510.49
113_M174_Q0.5510.49
84_V134_Q0.5490.49
320_V329_I0.5490.49
220_K256_A0.5460.49
101_Q135_V0.5460.49
269_R297_H0.5450.49
281_W332_A0.5440.48
326_G329_I0.5440.48
91_A153_K0.5440.48
265_G277_A0.5410.48
176_I179_S0.5410.48
97_A310_A0.5400.48
208_P211_K0.5400.48
107_T168_I0.5400.48
231_L237_G0.5400.48
123_K128_D0.5390.48
277_A298_G0.5380.48
214_P304_Y0.5370.48
82_I161_V0.5360.47
97_A100_A0.5340.47
129_D133_F0.5320.47
228_Q237_G0.5310.47
72_V124_G0.5280.46
203_K325_Q0.5280.46
69_N292_T0.5270.46
247_E253_V0.5260.46
132_M169_L0.5260.46
235_L271_K0.5260.46
65_Y125_S0.5250.46
81_V86_A0.5240.46
88_E136_Y0.5220.46
190_N196_T0.5210.46
213_S308_F0.5200.45
63_P72_V0.5190.45
128_D179_S0.5160.45
269_R303_Q0.5150.45
80_Y106_R0.5150.45
299_V305_G0.5120.44
113_M116_N0.5110.44
153_K157_I0.5090.44
134_Q303_Q0.5090.44
97_A101_Q0.5080.44
105_A248_T0.5070.44
219_T286_D0.5060.44
201_Y326_G0.5050.44
222_F287_T0.5050.44
180_F303_Q0.5050.44
138_S292_T0.5050.44
88_E182_S0.5040.43
116_N123_K0.5030.43
74_N332_A0.5030.43
155_K219_T0.5020.43
124_G197_S0.5020.43
239_S292_T0.5010.43
122_P132_M0.5010.43
85_V102_A0.5000.43
298_G303_Q0.5000.43
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4rwrA 1 0.7843 100 0.172 Contact Map
3behA 3 0.2915 18.1 0.974 Contact Map
1n7lA 1 0.0729 8.6 0.977 Contact Map
4ff5A 1 0.5335 6.9 0.978 Contact Map
5a2fA 1 0 6.3 0.979 Contact Map
4kytB 2 0.0641 5.4 0.979 Contact Map
4y3uB 1 0.0671 5.2 0.979 Contact Map
3oigA 4 0.1808 5.2 0.979 Contact Map
1fmhB 1 0.0845 5.1 0.98 Contact Map
3d1pA 1 0.2974 5 0.98 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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