GREMLIN Database
FUMC - Fumarate hydratase class II
UniProt: P07343 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 462 (402)
Sequences: 10894 (5824)
Seq/√Len: 290.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
59_K69_A4.0191.00
68_K131_D3.4921.00
60_R247_E3.4221.00
52_R77_D3.4121.00
56_L73_A3.3081.00
395_T398_E3.2771.00
46_A152_L2.9961.00
163_D210_K2.9171.00
56_L251_L2.8451.00
166_R207_D2.8121.00
283_A338_I2.7721.00
159_V214_L2.7201.00
168_T387_V2.6881.00
397_Q401_S2.6881.00
154_V275_A2.6691.00
42_R372_Q2.6571.00
444_T447_Q2.6311.00
156_E217_T2.6221.00
341_N370_S2.5411.00
159_V210_K2.5341.00
219_K274_Y2.5321.00
195_L391_P2.5011.00
247_E250_K2.4771.00
112_R116_L2.3911.00
172_K175_A2.3491.00
334_I377_G2.3361.00
65_D68_K2.3131.00
59_K66_A2.3001.00
151_V221_R2.2981.00
178_D393_K2.2111.00
221_R255_T2.2081.00
280_K342_D2.1811.00
398_E402_N2.1091.00
308_V390_E2.1001.00
163_D214_L2.0901.00
337_Q366_N2.0681.00
66_A70_E2.0661.00
161_A379_N2.0461.00
166_R210_K2.0371.00
431_L439_K1.9921.00
171_E387_V1.9911.00
247_E251_L1.9771.00
168_T382_H1.9631.00
171_E175_A1.9631.00
395_T399_N1.9571.00
425_L429_E1.9501.00
284_A339_M1.9481.00
290_A331_L1.9241.00
210_K214_L1.9171.00
168_T386_A1.9111.00
215_E219_K1.8861.00
212_M274_Y1.8421.00
49_I152_L1.8381.00
162_L282_L1.8321.00
215_E218_D1.7771.00
70_E74_A1.7741.00
170_D207_D1.7661.00
52_R73_A1.7641.00
441_E444_T1.7611.00
223_L249_T1.7601.00
453_K456_D1.7481.00
425_L440_L1.7461.00
56_L247_E1.7411.00
259_S265_A1.7371.00
176_Y179_I1.7281.00
418_N421_K1.7241.00
286_L331_L1.7221.00
222_A257_S1.7171.00
382_H387_V1.7031.00
166_R203_V1.6931.00
393_K397_Q1.6821.00
397_Q455_E1.6771.00
164_Q382_H1.6741.00
434_K449_N1.6671.00
445_E449_N1.6551.00
69_A73_A1.6491.00
447_Q450_E1.6411.00
209_S285_D1.6321.00
208_R264_H1.6131.00
225_I244_V1.6011.00
431_L436_A1.5811.00
52_R80_L1.5611.00
438_L445_E1.5541.00
273_T349_A1.5411.00
227_G265_A1.5231.00
344_T362_V1.5221.00
161_A375_S1.5051.00
57_A247_E1.5041.00
246_E250_K1.5021.00
173_A203_V1.4831.00
245_S249_T1.4661.00
172_K176_Y1.4601.00
218_D255_T1.4331.00
216_A278_A1.4321.00
446_E449_N1.4211.00
392_N395_T1.4211.00
153_A375_S1.4091.00
105_M109_V1.4061.00
328_S385_C1.3961.00
431_L435_E1.3801.00
77_D81_K1.3801.00
394_E398_E1.3761.00
208_R270_D1.3751.00
110_A113_A1.3611.00
269_H360_K1.3611.00
211_E219_K1.3491.00
45_K49_I1.3401.00
295_W353_N1.3261.00
199_I202_W1.3251.00
52_R56_L1.3241.00
343_A347_F1.3121.00
290_A381_F1.3071.00
164_Q387_V1.3031.00
418_N442_L1.3021.00
170_D174_K1.3021.00
280_K346_G1.2991.00
273_T281_A1.2951.00
150_A275_A1.2931.00
166_R206_L1.2921.00
432_T435_E1.2871.00
112_R115_A1.2851.00
277_G281_A1.2841.00
179_I393_K1.2741.00
42_R153_A1.2741.00
71_A74_A1.2691.00
45_K80_L1.2651.00
168_T171_E1.2651.00
159_V217_T1.2631.00
202_W355_E1.2571.00
47_F141_T1.2571.00
37_E369_Q1.2491.00
55_A64_L1.2481.00
306_E390_E1.2371.00
195_L199_I1.2321.00
306_E392_N1.2271.00
48_A80_L1.2041.00
162_L206_L1.2041.00
78_D112_R1.2021.00
337_Q369_Q1.2001.00
161_A382_H1.1821.00
174_K177_N1.1821.00
63_N134_R1.1771.00
135_S236_A1.1521.00
177_N196_G1.1451.00
170_D203_V1.1431.00
66_A69_A1.1421.00
176_Y393_K1.1371.00
331_L377_G1.1371.00
213_I282_L1.1351.00
159_V163_D1.1291.00
48_A105_M1.1291.00
45_K84_Y1.1281.00
345_I367_F1.1261.00
195_L396_I1.1231.00
249_T255_T1.1231.00
161_A164_Q1.1221.00
163_D167_N1.1181.00
78_D83_K1.1171.00
70_E73_A1.1081.00
48_A79_V1.1071.00
135_S237_H1.1061.00
53_S251_L1.1001.00
150_A367_F1.0991.00
151_V254_Q1.0971.00
164_Q379_N1.0961.00
414_I419_A1.0931.00
189_D300_P1.0911.00
202_W289_I1.0861.00
293_V328_S1.0851.00
77_D80_L1.0831.00
106_N109_V1.0751.00
272_I367_F1.0701.00
211_E218_D1.0661.00
335_A339_M1.0611.00
413_H447_Q1.0591.00
169_L289_I1.0591.00
165_L206_L1.0581.00
178_D196_G1.0581.00
339_M347_F1.0561.00
144_T223_L1.0531.00
73_A77_D1.0531.00
50_L141_T1.0471.00
153_A157_Q1.0461.00
181_K396_I1.0451.00
71_A75_V1.0411.00
192_P400_L1.0401.00
200_S261_N1.0381.00
429_E436_A1.0341.00
287_M332_T1.0291.00
60_R251_L1.0291.00
340_G344_T1.0251.00
75_V78_D1.0241.00
41_M85_D1.0221.00
46_A148_V1.0201.00
146_M364_I1.0181.00
154_V217_T1.0161.00
165_L386_A1.0151.00
278_A282_L1.0131.00
172_K386_A1.0051.00
221_R254_Q0.9981.00
49_I251_L0.9981.00
106_N110_A0.9971.00
172_K199_I0.9971.00
177_N194_T0.9961.00
244_V249_T0.9941.00
286_L378_M0.9931.00
45_K152_L0.9881.00
157_Q375_S0.9851.00
146_M272_I0.9791.00
59_K70_E0.9761.00
154_V213_I0.9761.00
412_P447_Q0.9701.00
413_H451_M0.9691.00
186_H329_E0.9681.00
151_V217_T0.9671.00
179_I396_I0.9671.00
41_M45_K0.9651.00
308_V392_N0.9611.00
425_L436_A0.9591.00
183_G193_L0.9591.00
312_N384_K0.9571.00
293_V389_I0.9561.00
431_L440_L0.9561.00
193_L230_V0.9551.00
65_D131_D0.9541.00
275_A367_F0.9491.00
189_D299_G0.9471.00
74_A116_L0.9411.00
342_D346_G0.9401.00
217_T221_R0.9391.00
204_H208_R0.9371.00
109_V113_A0.9351.00
162_L210_K0.9341.00
61_L237_H0.9321.00
92_V114_T0.9301.00
113_A116_L0.9301.00
215_E274_Y0.9271.00
276_H345_I0.9271.00
307_I389_I0.9221.00
295_W354_F0.9211.00
50_L144_T0.9201.00
42_R152_L0.9131.00
435_E439_K0.9111.00
67_E70_E0.9091.00
89_P92_V0.9071.00
113_A126_I0.9031.00
211_E215_E0.9001.00
223_L248_I0.8981.00
169_L199_I0.8971.00
91_V94_Q0.8931.00
57_A61_L0.8931.00
184_R189_D0.8911.00
175_A390_E0.8911.00
92_V95_T0.8901.00
47_F50_L0.8871.00
193_L263_F0.8841.00
68_K126_I0.8821.00
71_A128_P0.8811.00
49_I80_L0.8791.00
436_A440_L0.8751.00
78_D82_G0.8751.00
166_R170_D0.8691.00
446_E450_E0.8651.00
276_H367_F0.8641.00
127_H130_D0.8611.00
197_Q262_K0.8611.00
81_K85_D0.8601.00
337_Q365_Y0.8571.00
78_D124_Q0.8551.00
107_E113_A0.8521.00
218_D221_R0.8511.00
74_A78_D0.8511.00
84_Y112_R0.8501.00
303_G395_T0.8491.00
400_L454_P0.8481.00
448_F452_V0.8481.00
284_A349_A0.8471.00
49_I248_I0.8441.00
198_E355_E0.8421.00
46_A149_A0.8411.00
82_G112_R0.8371.00
276_H342_D0.8361.00
173_A200_S0.8351.00
179_I391_P0.8351.00
189_D403_S0.8331.00
90_L93_W0.8331.00
78_D116_L0.8321.00
152_L156_E0.8301.00
434_K445_E0.8281.00
434_K438_L0.8261.00
204_H260_P0.8241.00
169_L203_V0.8211.00
76_C79_V0.8211.00
68_K72_I0.8201.00
187_L295_W0.8201.00
307_I388_G0.8191.00
167_N171_E0.8181.00
146_M367_F0.8181.00
180_V400_L0.8181.00
203_V207_D0.8141.00
168_T172_K0.8121.00
365_Y369_Q0.8111.00
169_L386_A0.8091.00
437_A440_L0.8081.00
194_T458_V0.8061.00
110_A114_T0.8030.99
425_L439_K0.8010.99
228_T318_I0.8000.99
78_D84_Y0.7990.99
67_E71_A0.7980.99
45_K85_D0.7970.99
222_A271_E0.7960.99
55_A69_A0.7940.99
198_E296_L0.7920.99
282_L378_M0.7910.99
331_L381_F0.7900.99
54_T136_Q0.7890.99
187_L298_S0.7890.99
341_N369_Q0.7880.99
287_M350_S0.7850.99
176_Y391_P0.7830.99
289_I381_F0.7760.99
277_G342_D0.7750.99
401_S434_K0.7690.99
106_N113_A0.7680.99
339_M343_A0.7670.99
59_K64_L0.7660.99
184_R300_P0.7660.99
268_S285_D0.7640.99
60_R66_A0.7620.99
421_K425_L0.7620.99
267_T355_E0.7610.99
57_A244_V0.7590.99
441_E445_E0.7540.99
208_R222_A0.7540.99
274_Y277_G0.7540.99
336_A348_A0.7500.99
68_K128_P0.7500.99
447_Q451_M0.7490.99
90_L94_Q0.7460.99
87_N91_V0.7450.99
89_P93_W0.7420.99
308_V388_G0.7400.99
336_A344_T0.7350.99
181_K198_E0.7330.99
154_V216_A0.7310.99
337_Q341_N0.7300.99
222_A259_S0.7290.99
224_A265_A0.7290.99
104_N109_V0.7280.99
47_F105_M0.7280.99
258_S262_K0.7260.99
310_P388_G0.7220.99
56_L60_R0.7200.99
270_D285_D0.7150.99
162_L378_M0.7150.99
82_G87_N0.7150.99
426_A429_E0.7140.99
344_T366_N0.7140.99
343_A346_G0.7130.99
72_I128_P0.7130.99
109_V112_R0.7100.99
290_A328_S0.7090.99
246_E249_T0.7070.99
263_F355_E0.7050.99
180_V458_V0.7030.99
296_L354_F0.7030.99
175_A393_K0.7030.99
385_C389_I0.7020.99
275_A372_Q0.7000.99
391_P396_I0.7000.99
245_S258_S0.6990.99
60_R237_H0.6990.99
52_R251_L0.6970.99
43_V142_F0.6940.99
103_M106_N0.6940.99
409_A413_H0.6930.98
53_S56_L0.6930.98
84_Y87_N0.6920.98
201_G288_K0.6910.98
341_N371_V0.6910.98
182_I302_C0.6900.98
436_A439_K0.6890.98
275_A279_L0.6890.98
421_K440_L0.6880.98
80_L84_Y0.6880.98
284_A346_G0.6870.98
206_L378_M0.6860.98
272_I345_I0.6850.98
257_S260_P0.6850.98
157_Q161_A0.6850.98
303_G392_N0.6850.98
111_N115_A0.6840.98
179_I194_T0.6830.98
114_T129_N0.6830.98
79_V109_V0.6820.98
169_L206_L0.6810.98
102_N310_P0.6800.98
64_L134_R0.6800.98
348_A362_V0.6790.98
339_M346_G0.6780.98
158_L371_V0.6760.98
287_M351_Q0.6760.98
367_F371_V0.6730.98
39_M365_Y0.6730.98
94_Q108_V0.6720.98
443_L451_M0.6720.98
414_I418_N0.6710.98
432_T436_A0.6710.98
435_E445_E0.6700.98
394_E397_Q0.6700.98
221_R249_T0.6690.98
72_I76_C0.6690.98
308_V311_E0.6680.98
147_H271_E0.6680.98
206_L209_S0.6670.98
154_V159_V0.6670.98
264_H270_D0.6630.98
223_L258_S0.6630.98
205_M285_D0.6610.98
167_N170_D0.6590.98
212_M270_D0.6560.98
378_M382_H0.6550.98
52_R69_A0.6540.98
199_I396_I0.6530.98
37_E365_Y0.6530.98
408_T411_N0.6530.98
205_M268_S0.6520.98
337_Q373_L0.6520.98
225_I248_I0.6520.98
289_I386_A0.6520.98
420_A424_K0.6500.98
163_D207_D0.6480.98
438_L448_F0.6470.98
158_L375_S0.6460.98
128_P131_D0.6460.98
344_T348_A0.6460.98
104_N107_E0.6440.98
56_L69_A0.6420.98
88_F94_Q0.6390.97
165_L382_H0.6390.97
176_Y195_L0.6380.97
156_E254_Q0.6380.97
63_N237_H0.6370.97
151_V255_T0.6370.97
286_L382_H0.6350.97
179_I397_Q0.6350.97
151_V156_E0.6330.97
178_D455_E0.6320.97
163_D166_R0.6320.97
171_E174_K0.6320.97
395_T402_N0.6310.97
291_N332_T0.6300.97
154_V220_M0.6290.97
107_E110_A0.6280.97
149_A371_V0.6280.97
410_L448_F0.6280.97
59_K73_A0.6260.97
299_G303_G0.6260.97
327_Q381_F0.6250.97
160_P164_Q0.6220.97
455_E459_K0.6220.97
284_A350_S0.6200.97
181_K193_L0.6200.97
280_K284_A0.6180.97
401_S432_T0.6180.97
108_V113_A0.6180.97
282_L374_L0.6170.97
114_T125_T0.6170.97
182_I400_L0.6160.97
86_D89_P0.6140.97
248_I251_L0.6140.97
287_M347_F0.6120.97
209_S281_A0.6120.97
164_Q168_T0.6100.97
264_H285_D0.6100.97
79_V85_D0.6100.97
305_G392_N0.6100.97
275_A371_V0.6060.97
88_F108_V0.6060.97
55_A132_V0.6040.96
55_A72_I0.6040.96
158_L279_L0.6030.96
399_N402_N0.6030.96
212_M215_E0.6020.96
63_N135_S0.5980.96
273_T277_G0.5980.96
331_L385_C0.5930.96
165_L378_M0.5920.96
413_H417_E0.5920.96
64_L68_K0.5910.96
88_F93_W0.5910.96
48_A76_C0.5890.96
216_A275_A0.5880.96
211_E214_L0.5880.96
68_K71_A0.5870.96
429_E439_K0.5870.96
191_T230_V0.5870.96
153_A371_V0.5860.96
205_M267_T0.5850.96
349_A360_K0.5850.96
82_G116_L0.5840.96
174_K178_D0.5820.96
108_V112_R0.5810.96
173_A177_N0.5810.96
206_L286_L0.5800.96
69_A72_I0.5800.96
87_N90_L0.5790.95
216_A220_M0.5790.95
312_N388_G0.5780.95
327_Q385_C0.5780.95
279_L374_L0.5770.95
202_W205_M0.5770.95
105_M110_A0.5770.95
212_M219_K0.5760.95
172_K389_I0.5760.95
107_E112_R0.5750.95
273_T345_I0.5740.95
107_E111_N0.5730.95
206_L282_L0.5710.95
332_T359_F0.5690.95
64_L72_I0.5680.95
183_G230_V0.5680.95
366_N369_Q0.5670.95
431_L437_A0.5650.95
393_K396_I0.5650.95
104_N110_A0.5650.95
279_L282_L0.5610.95
280_K343_A0.5600.95
82_G124_Q0.5600.95
125_T128_P0.5590.95
170_D173_A0.5590.95
400_L405_M0.5570.94
425_L431_L0.5560.94
211_E222_A0.5560.94
94_Q111_N0.5540.94
127_H417_E0.5530.94
48_A85_D0.5530.94
83_K88_F0.5530.94
89_P94_Q0.5530.94
283_A374_L0.5530.94
229_A318_I0.5480.94
108_V111_N0.5480.94
209_S212_M0.5470.94
131_D134_R0.5460.94
162_L213_I0.5460.94
46_A49_I0.5460.94
158_L374_L0.5450.94
159_V278_A0.5440.94
189_D405_M0.5430.94
181_K296_L0.5430.94
450_E456_D0.5430.94
277_G280_K0.5420.94
42_R157_Q0.5410.94
450_E453_K0.5410.94
218_D222_A0.5410.94
105_M108_V0.5400.94
165_L168_T0.5370.93
50_L145_A0.5370.93
54_T248_I0.5370.93
427_H430_G0.5360.93
64_L69_A0.5350.93
213_I278_A0.5340.93
204_H207_D0.5330.93
295_W302_C0.5330.93
59_K65_D0.5320.93
207_D211_E0.5320.93
114_T124_Q0.5320.93
82_G85_D0.5310.93
364_I368_L0.5310.93
49_I53_S0.5300.93
291_N351_Q0.5300.93
422_I440_L0.5290.93
198_E292_D0.5290.93
409_A412_P0.5270.93
161_A380_S0.5260.93
214_L218_D0.5240.92
336_A347_F0.5240.92
334_I373_L0.5230.92
438_L450_E0.5210.92
290_A332_T0.5200.92
109_V132_V0.5180.92
435_E438_L0.5180.92
186_H358_V0.5160.92
199_I389_I0.5150.92
48_A82_G0.5130.92
425_L428_K0.5130.92
274_Y281_A0.5130.92
216_A274_Y0.5130.92
61_L247_E0.5120.92
49_I252_T0.5120.92
293_V381_F0.5100.91
193_L197_Q0.5090.91
417_E421_K0.5090.91
65_D69_A0.5080.91
150_A220_M0.5080.91
313_E326_T0.5080.91
438_L449_N0.5080.91
55_A73_A0.5070.91
279_L338_I0.5050.91
244_V248_I0.5050.91
81_K84_Y0.5000.91
338_I373_L0.5000.91
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1furA 4 0.987 100 0.118 Contact Map
1vdkA 4 0.987 100 0.122 Contact Map
1yfmA 4 0.9675 100 0.125 Contact Map
3gtdA 4 0.9827 100 0.138 Contact Map
4hgvA 4 0.9935 100 0.139 Contact Map
3r6qA 4 0.9913 100 0.139 Contact Map
3e04A 4 0.9935 100 0.142 Contact Map
1jswA 4 0.9827 100 0.143 Contact Map
4admA 4 0.9805 100 0.153 Contact Map
3ocfA 4 0.9654 100 0.156 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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