SP0A - BACSU - GREMLIN Contacts

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SP0A - Stage 0 sporulation protein A

UniProt:	P06534	Sequences: MEKIKVCVADDNRELVSLLSEYIEGQEDMEVIGVAYNGQECLSLFKEKDPDVLVLDIIMPHLDGLAVLERLRESDLKKQPNVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNASSVTHRAPSSQSSIIRSSQPEPKKKNLDASITSIIHEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVSMTKAKPTNSEFIAMVADKLRLEHKAS	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	267 (226)
Sequences:	19562 (16467)
Seq/√Len:	1095.4

ECOLI - CHEB RSSB BARA RCSC CREB TORS ARCA YEHT BASR RSTA ARCB QSEB DCUR PHOP YEDW DPIA YGEK YPDB FIMZ RCSB CUSR EVGS UVRY NARP YHJB PHOB OMPR NARL KDPE TORR EVGA NTRC BAER CPXR YJJQ GLRR BGLJ CSGD UHPA ATOC ZRAR RCSA RCSD
BACSU - SP0A YESN GLNL CHEB YKOG YVRH MALR DEGU CITT LYTT YXJL YFIK PHOP RESD YCLJ YYCF YXDJ NATR YVFU CMPA LIAR DCTR YHCZ YRKP BCER YVCP YCBL CSSR YBDJ YDFI DESR CHEV
HALSA - CHEB Q9HR09 BAT O51970 Q9HRE5 Q9HNM3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
16_V	36_Y	3.385	1.00
16_V	34_V	3.314	1.00
20_S	34_V	3.232	1.00
75_D	78_K	2.911	1.00
9_A	41_C	2.887	1.00
144_S	151_K	2.856	1.00
46_K	74_S	2.758	1.00
42_L	70_R	2.682	1.00
69_E	100_L	2.606	1.00
38_G	62_L	2.564	1.00
39_Q	61_H	2.430	1.00
55_L	67_V	2.424	1.00
63_D	66_A	2.387	1.00
33_G	50_P	2.336	1.00
5_K	30_E	2.212	1.00
160_I	165_G	2.166	1.00
37_N	61_H	2.083	1.00
70_R	74_S	2.072	1.00
45_F	71_L	2.037	1.00
33_G	44_L	2.004	1.00
35_A	44_L	1.999	1.00
81_N	129_V	1.988	1.00
103_S	122_V	1.985	1.00
7_C	35_A	1.966	1.00
68_L	82_V	1.965	1.00
26_Q	116_V	1.963	1.00
36_Y	40_E	1.962	1.00
104_Y	118_H	1.932	1.00
33_G	48_K	1.919	1.00
70_R	73_E	1.907	1.00
37_N	40_E	1.838	1.00
62_L	70_R	1.725	1.00
58_I	88_F	1.720	1.00
44_L	48_K	1.709	1.00
81_N	125_N	1.646	1.00
5_K	33_G	1.621	1.00
155_A	160_I	1.616	1.00
96_K	100_L	1.610	1.00
38_G	67_V	1.603	1.00
44_L	50_P	1.598	1.00
22_Y	112_M	1.583	1.00
5_K	48_K	1.556	1.00
83_I	118_H	1.537	1.00
68_L	84_M	1.508	1.00
159_S	163_E	1.507	1.00
103_S	125_N	1.474	1.00
44_L	47_E	1.466	1.00
62_L	66_A	1.459	1.00
53_L	71_L	1.456	1.00
104_Y	121_Q	1.437	1.00
41_C	67_V	1.419	1.00
75_D	80_P	1.416	1.00
69_E	96_K	1.415	1.00
126_A	130_T	1.402	1.00
66_A	69_E	1.401	1.00
6_V	29_M	1.400	1.00
20_S	24_E	1.381	1.00
84_M	97_A	1.358	1.00
10_D	16_V	1.347	1.00
65_L	97_A	1.343	1.00
53_L	80_P	1.336	1.00
54_V	119_I	1.331	1.00
219_A	222_V	1.301	1.00
225_S	235_L	1.298	1.00
117_G	121_Q	1.287	1.00
132_R	135_S	1.270	1.00
53_L	82_V	1.265	1.00
55_L	82_V	1.249	1.00
96_K	99_D	1.241	1.00
42_L	62_L	1.240	1.00
39_Q	43_S	1.238	1.00
57_I	84_M	1.234	1.00
11_D	56_D	1.223	1.00
35_A	41_C	1.223	1.00
84_M	102_A	1.222	1.00
215_A	219_A	1.213	1.00
156_S	159_S	1.208	1.00
71_L	82_V	1.205	1.00
160_I	163_E	1.202	1.00
42_L	67_V	1.187	1.00
68_L	102_A	1.185	1.00
200_D	233_S	1.181	1.00
52_V	119_I	1.161	1.00
132_R	136_S	1.151	1.00
55_L	84_M	1.133	1.00
128_S	132_R	1.131	1.00
43_S	47_E	1.130	1.00
72_R	103_S	1.118	1.00
52_V	123_S	1.114	1.00
57_I	65_L	1.107	1.00
114_N	118_H	1.104	1.00
23_I	119_I	1.101	1.00
15_L	18_L	1.094	1.00
95_K	99_D	1.083	1.00
196_V	200_D	1.081	1.00
214_R	218_H	1.080	1.00
91_E	94_T	1.077	1.00
69_E	73_E	1.076	1.00
116_V	120_R	1.066	1.00
57_I	93_V	1.061	1.00
40_E	43_S	1.058	1.00
15_L	110_F	1.054	1.00
39_Q	62_L	1.052	1.00
99_D	104_Y	1.051	1.00
159_S	162_H	1.024	1.00
43_S	46_K	1.020	1.00
42_L	46_K	1.020	1.00
8_V	19_L	1.017	1.00
54_V	115_L	1.014	1.00
25_G	28_D	1.012	1.00
212_V	236_F	1.011	1.00
153_L	156_S	0.991	1.00
17_S	20_S	0.990	1.00
5_K	50_P	0.988	1.00
81_N	126_A	0.985	1.00
13_R	17_S	0.980	1.00
66_A	70_R	0.978	1.00
118_H	122_V	0.976	1.00
110_F	115_L	0.975	1.00
124_G	127_S	0.974	1.00
29_M	120_R	0.970	1.00
139_S	142_R	0.968	1.00
213_E	216_I	0.966	1.00
65_L	96_K	0.965	1.00
81_N	103_S	0.965	1.00
51_D	126_A	0.961	1.00
9_A	53_L	0.957	1.00
67_V	71_L	0.956	1.00
17_S	34_V	0.955	1.00
212_V	240_V	0.949	1.00
55_L	68_L	0.942	1.00
83_I	122_V	0.940	1.00
21_E	25_G	0.940	1.00
35_A	38_G	0.936	1.00
91_E	95_K	0.935	1.00
114_N	117_G	0.933	1.00
92_D	95_K	0.931	1.00
55_L	71_L	0.927	1.00
38_G	59_M	0.922	1.00
135_S	139_S	0.919	1.00
35_A	40_E	0.916	1.00
26_Q	120_R	0.916	1.00
9_A	67_V	0.915	1.00
123_S	127_S	0.914	1.00
7_C	50_P	0.913	1.00
194_T	236_F	0.912	1.00
38_G	42_L	0.901	1.00
7_C	45_F	0.901	1.00
131_H	136_S	0.896	1.00
17_S	21_E	0.896	1.00
187_I	191_G	0.892	1.00
125_N	129_V	0.891	1.00
45_F	50_P	0.891	1.00
45_F	80_P	0.889	1.00
5_K	51_D	0.889	1.00
136_S	139_S	0.885	1.00
19_L	115_L	0.885	1.00
54_V	83_I	0.882	1.00
139_S	143_S	0.881	1.00
174_L	177_R	0.870	1.00
191_G	217_R	0.869	1.00
75_D	79_Q	0.867	1.00
7_C	44_L	0.865	1.00
214_R	217_R	0.864	1.00
12_N	15_L	0.861	1.00
59_M	62_L	0.853	1.00
131_H	135_S	0.852	1.00
9_A	38_G	0.852	1.00
41_C	45_F	0.851	1.00
223_A	226_R	0.847	1.00
124_G	128_S	0.846	1.00
57_I	63_D	0.846	1.00
187_I	224_W	0.845	1.00
40_E	44_L	0.842	1.00
217_R	221_E	0.837	1.00
72_R	81_N	0.833	1.00
13_R	16_V	0.829	1.00
216_I	220_I	0.827	1.00
158_T	162_H	0.823	1.00
10_D	59_M	0.820	1.00
19_L	85_L	0.816	1.00
111_D	114_N	0.815	1.00
89_G	107_L	0.813	1.00
22_Y	116_V	0.810	1.00
152_N	156_S	0.809	1.00
58_I	90_Q	0.808	1.00
93_V	96_K	0.802	1.00
118_H	121_Q	0.802	1.00
9_A	59_M	0.800	1.00
45_F	75_D	0.796	1.00
191_G	195_K	0.795	1.00
85_L	110_F	0.791	1.00
90_Q	93_V	0.788	1.00
46_K	70_R	0.787	1.00
68_L	100_L	0.787	1.00
7_C	41_C	0.785	1.00
79_Q	129_V	0.783	1.00
112_M	116_V	0.781	1.00
158_T	161_I	0.771	1.00
138_S	142_R	0.765	1.00
194_T	212_V	0.764	1.00
31_V	34_V	0.756	1.00
94_T	114_N	0.754	1.00
9_A	35_A	0.754	1.00
182_M	186_D	0.754	1.00
227_G	231_S	0.752	1.00
71_L	80_P	0.751	1.00
120_R	124_G	0.750	1.00
54_V	85_L	0.749	1.00
53_L	67_V	0.746	1.00
8_V	20_S	0.744	1.00
37_N	59_M	0.744	1.00
195_K	199_P	0.735	1.00
135_S	138_S	0.731	1.00
113_E	117_G	0.729	1.00
134_P	138_S	0.729	1.00
45_F	53_L	0.726	1.00
191_G	221_E	0.715	1.00
212_V	233_S	0.714	1.00
81_N	122_V	0.712	1.00
14_E	18_L	0.711	1.00
58_I	63_D	0.708	1.00
94_T	98_V	0.706	1.00
207_T	210_S	0.705	1.00
192_S	196_V	0.700	1.00
55_L	59_M	0.697	1.00
140_I	143_S	0.690	1.00
187_I	190_L	0.690	1.00
218_H	221_E	0.685	1.00
123_S	126_A	0.684	1.00
8_V	31_V	0.681	1.00
57_I	97_A	0.681	1.00
20_S	31_V	0.680	1.00
88_F	93_V	0.680	1.00
179_A	185_N	0.676	1.00
59_M	67_V	0.675	1.00
223_A	231_S	0.670	1.00
8_V	16_V	0.668	1.00
11_D	59_M	0.665	1.00
136_S	140_I	0.665	1.00
92_D	96_K	0.665	1.00
106_I	110_F	0.659	1.00
38_G	61_H	0.656	1.00
7_C	33_G	0.655	1.00
79_Q	133_A	0.654	1.00
213_E	217_R	0.651	1.00
93_V	105_F	0.650	1.00
202_A	210_S	0.647	1.00
129_V	132_R	0.647	1.00
89_G	94_T	0.646	1.00
155_A	158_T	0.644	1.00
219_A	223_A	0.643	1.00
117_G	120_R	0.641	1.00
191_G	194_T	0.641	1.00
62_L	67_V	0.640	1.00
212_V	216_I	0.639	1.00
197_L	233_S	0.638	1.00
52_V	81_N	0.637	1.00
58_I	87_A	0.637	1.00
56_D	108_K	0.634	1.00
153_L	160_I	0.629	1.00
133_A	136_S	0.628	1.00
18_L	22_Y	0.627	1.00
152_N	155_A	0.626	1.00
235_L	239_T	0.624	1.00
186_D	189_L	0.621	1.00
11_D	87_A	0.621	1.00
199_P	203_K	0.619	1.00
128_S	131_H	0.610	1.00
72_R	80_P	0.610	1.00
212_V	217_R	0.607	1.00
125_N	128_S	0.606	1.00
97_A	102_A	0.600	1.00
30_E	33_G	0.597	1.00
197_L	201_I	0.597	1.00
65_L	93_V	0.593	1.00
138_S	143_S	0.592	1.00
71_L	74_S	0.590	1.00
201_I	205_F	0.588	1.00
233_S	237_G	0.588	1.00
210_S	214_R	0.587	1.00
225_S	228_N	0.584	1.00
23_I	116_V	0.581	1.00
87_A	109_P	0.578	1.00
29_M	116_V	0.578	1.00
6_V	23_I	0.574	1.00
16_V	20_S	0.573	1.00
13_R	36_Y	0.572	1.00
196_V	199_P	0.570	1.00
164_I	183_V	0.565	1.00
218_H	222_V	0.564	1.00
154_D	161_I	0.563	1.00
98_V	102_A	0.561	1.00
63_D	96_K	0.561	1.00
45_F	67_V	0.559	1.00
131_H	134_P	0.558	1.00
84_M	100_L	0.558	1.00
135_S	143_S	0.554	1.00
181_S	185_N	0.554	1.00
11_D	86_T	0.552	1.00
29_M	123_S	0.552	1.00
134_P	137_Q	0.550	1.00
61_H	67_V	0.544	1.00
133_A	137_Q	0.542	1.00
129_V	133_A	0.541	1.00
85_L	88_F	0.540	1.00
26_Q	29_M	0.540	1.00
207_T	211_R	0.538	1.00
155_A	159_S	0.537	1.00
28_D	31_V	0.537	1.00
236_F	239_T	0.536	1.00
52_V	83_I	0.535	1.00
49_D	75_D	0.534	1.00
156_S	160_I	0.531	1.00
74_S	78_K	0.531	1.00
198_Y	202_A	0.530	1.00
160_I	166_V	0.528	1.00
98_V	104_Y	0.527	1.00
200_D	204_K	0.527	1.00
103_S	118_H	0.527	1.00
228_N	231_S	0.522	1.00
30_E	48_K	0.521	1.00
50_P	80_P	0.520	1.00
58_I	93_V	0.520	1.00
68_L	72_R	0.520	1.00
126_A	129_V	0.518	1.00
156_S	164_I	0.518	1.00
119_I	123_S	0.518	1.00
202_A	208_T	0.518	1.00
12_N	87_A	0.516	1.00
228_N	232_I	0.512	1.00
9_A	55_L	0.508	1.00
153_L	166_V	0.508	1.00
231_S	234_S	0.506	1.00
216_I	219_A	0.503	1.00
6_V	54_V	0.502	1.00
39_Q	70_R	0.501	1.00
137_Q	141_I	0.501	1.00
215_A	218_H	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3breA	4	0.985	100	0.446	Contact Map
3dzdA	2	0.9101	100	0.456	Contact Map
4g97A	1	0.4906	100	0.466	Contact Map
3n0rA	1	0.4607	100	0.467	Contact Map
4l4uA	2	0.9438	100	0.467	Contact Map
3rqiA	2	0.633	99.9	0.479	Contact Map
1yioA	1	0.7416	99.9	0.481	Contact Map
3lufA	2	0.4757	99.9	0.487	Contact Map
3eq2A	2	0.9176	99.9	0.488	Contact Map
1s8nA	1	0.7079	99.9	0.49	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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