GREMLIN Database
LEU3 - 3-isopropylmalate dehydrogenase
UniProt: P05645 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 365 (333)
Sequences: 6926 (3093)
Seq/√Len: 169.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
181_A215_D4.1451.00
174_R212_E3.8421.00
173_I206_A3.0841.00
20_S349_T2.9721.00
174_R178_K2.8671.00
4_R37_E2.7311.00
23_D350_E2.7091.00
225_N229_Q2.6371.00
329_S355_A2.6131.00
307_R320_E2.6031.00
325_K355_A2.4251.00
26_K38_F2.4041.00
57_E61_A2.3361.00
351_E354_A2.3251.00
166_R170_E2.2641.00
18_L298_A2.2581.00
7_L22_T2.1941.00
20_S23_D2.1921.00
307_R317_K2.1601.00
188_T219_E2.1061.00
41_G62_A2.0861.00
206_A218_L2.0831.00
53_N57_E2.0571.00
186_K219_E2.0451.00
22_T40_Y2.0221.00
326_V355_A2.0001.00
131_V179_M1.9761.00
170_E208_D1.9551.00
6_A62_A1.9541.00
42_L50_E1.9411.00
296_F352_V1.9361.00
64_K99_L1.9291.00
173_I241_V1.9181.00
300_L323_V1.9151.00
312_L315_E1.9121.00
170_E205_V1.8941.00
186_K217_K1.8891.00
299_I352_V1.8751.00
291_G344_S1.8611.00
178_K213_F1.8611.00
233_A236_Q1.8341.00
47_A56_P1.8041.00
61_A65_N1.8011.00
291_G346_Q1.7971.00
317_K321_D1.7911.00
15_P19_E1.7731.00
13_I293_A1.7511.00
207_E220_H1.7421.00
166_R208_D1.7211.00
174_R213_F1.7181.00
208_D211_Q1.7151.00
303_A320_E1.7121.00
349_T353_K1.7091.00
331_K351_E1.6901.00
109_K324_N1.6641.00
277_F309_S1.6571.00
190_V226_A1.6561.00
64_K98_Q1.6471.00
313_E317_K1.6201.00
34_H312_L1.6171.00
170_E174_R1.6151.00
156_Q166_R1.6071.00
205_V208_D1.5801.00
350_E354_A1.5691.00
102_F271_S1.5591.00
162_L243_E1.5491.00
112_E328_A1.5261.00
61_A64_K1.5091.00
344_S347_A1.5071.00
105_L131_V1.5061.00
3_K312_L1.4991.00
147_K204_E1.4941.00
6_A66_A1.4891.00
28_V319_V1.4881.00
60_V98_Q1.4711.00
39_E65_N1.4591.00
21_A302_A1.4581.00
231_I253_E1.4581.00
307_R316_A1.4461.00
209_V212_E1.4381.00
94_S98_Q1.4361.00
46_A50_E1.4281.00
8_L43_I1.4241.00
174_R209_V1.4221.00
133_V175_E1.4171.00
189_S206_A1.4141.00
6_A41_G1.4091.00
23_D353_K1.4021.00
178_K182_A1.3911.00
38_F302_A1.3851.00
39_E66_A1.3801.00
34_H315_E1.3761.00
353_K356_I1.3691.00
22_T26_K1.3661.00
291_G345_T1.3651.00
155_E167_T1.3591.00
184_K238_D1.3551.00
132_I251_S1.3541.00
25_L36_F1.3531.00
27_S353_K1.3481.00
9_P40_Y1.3461.00
162_L198_S1.3361.00
113_S328_A1.3171.00
307_R313_E1.3151.00
76_G79_W1.3131.00
42_L51_H1.2871.00
21_A70_L1.2801.00
296_F348_I1.2651.00
42_L47_A1.2621.00
292_M334_R1.2611.00
326_V351_E1.2601.00
221_M229_Q1.2551.00
227_A250_L1.2541.00
55_L60_V1.2471.00
133_V179_M1.2461.00
189_S220_H1.2321.00
305_L310_F1.2201.00
122_K126_D1.2141.00
173_I209_V1.2131.00
22_T38_F1.2101.00
201_L205_V1.2061.00
21_A298_A1.2061.00
178_K181_A1.1991.00
39_E62_A1.1941.00
185_G238_D1.1941.00
177_F213_F1.1881.00
3_K310_F1.1851.00
221_M225_N1.1831.00
63_C69_I1.1821.00
321_D325_K1.1801.00
221_M237_F1.1721.00
170_E209_V1.1721.00
306_L315_E1.1701.00
207_E210_A1.1661.00
348_I352_V1.1571.00
24_V319_V1.1421.00
4_R39_E1.1421.00
132_I254_A1.1341.00
7_L40_Y1.1341.00
221_M226_A1.1301.00
230_L240_V1.1281.00
204_E208_D1.1211.00
47_A51_H1.1201.00
166_R205_V1.1171.00
351_E355_A1.1161.00
109_K129_D1.1151.00
130_F230_L1.1111.00
160_D198_S1.1091.00
347_A350_E1.0941.00
51_H58_E1.0821.00
177_F216_V1.0821.00
183_R238_D1.0781.00
337_A340_E1.0711.00
6_A63_C1.0691.00
100_D171_R1.0681.00
112_E126_D1.0681.00
105_L133_V1.0641.00
227_A253_E1.0641.00
169_I173_I1.0580.99
28_V315_E1.0550.99
25_L302_A1.0500.99
70_L302_A1.0430.99
318_A356_I1.0420.99
287_I293_A1.0420.99
127_N235_N1.0420.99
187_V218_L1.0400.99
325_K329_S1.0370.99
223_V249_I1.0350.99
23_D346_Q1.0300.99
157_E163_F1.0210.99
145_P161_T1.0190.99
71_L92_L1.0170.99
193_A203_R1.0140.99
291_G347_A1.0130.99
20_S346_Q1.0050.99
19_E23_D1.0020.99
140_L243_E0.9960.99
140_L162_L0.9950.99
167_T170_E0.9940.99
164_Y243_E0.9900.99
296_F327_L0.9900.99
179_M183_R0.9900.99
136_L268_S0.9900.99
190_V223_V0.9880.99
58_E61_A0.9870.99
181_A185_G0.9850.99
229_Q236_Q0.9820.99
21_A299_I0.9780.99
23_D349_T0.9780.99
158_A201_L0.9690.99
4_R65_N0.9660.99
176_G187_V0.9630.99
219_E236_Q0.9610.99
181_A216_V0.9580.99
56_P59_T0.9550.99
24_V356_I0.9520.99
47_A59_T0.9510.99
177_F210_A0.9500.99
49_D78_K0.9480.99
315_E318_A0.9470.99
300_L320_E0.9470.99
232_Y256_M0.9470.99
206_A241_V0.9450.99
24_V353_K0.9330.99
187_V209_V0.9330.99
130_F254_A0.9310.99
191_D203_R0.9270.99
287_I292_M0.9210.99
139_G162_L0.9200.99
103_A172_V0.9160.99
53_N94_S0.9090.98
296_F323_V0.9060.98
187_V239_V0.9010.98
270_S274_L0.9000.98
109_K320_E0.8990.98
28_V302_A0.8970.98
16_E289_G0.8970.98
177_F187_V0.8940.98
326_V331_K0.8930.98
7_L18_L0.8920.98
164_Y169_I0.8920.98
9_P19_E0.8910.98
17_V298_A0.8900.98
20_S291_G0.8900.98
279_P298_A0.8860.98
41_G66_A0.8760.98
229_Q233_A0.8750.98
181_A213_F0.8730.98
317_K320_E0.8720.98
58_E62_A0.8690.98
162_L199_S0.8670.98
139_G243_E0.8650.98
333_T348_I0.8610.98
24_V299_I0.8610.98
17_V72_G0.8600.98
325_K328_A0.8600.98
62_A66_A0.8580.98
287_I334_R0.8570.98
105_L175_E0.8570.98
340_E347_A0.8550.98
326_V352_V0.8540.98
327_L332_R0.8540.98
189_S218_L0.8480.98
41_G59_T0.8460.98
303_A307_R0.8450.98
103_A175_E0.8440.97
187_V241_V0.8410.97
68_A310_F0.8400.97
113_S324_N0.8390.97
7_L38_F0.8380.97
43_I95_I0.8380.97
299_I323_V0.8370.97
152_T155_E0.8350.97
111_F324_N0.8280.97
199_S246_F0.8270.97
108_V254_A0.8220.97
286_D334_R0.8200.97
3_K34_H0.8140.97
28_V34_H0.8130.97
18_L72_G0.8110.97
100_D137_T0.8110.97
190_V240_V0.8100.97
49_D76_G0.8090.97
20_S350_E0.8090.97
191_D220_H0.8020.97
334_R344_S0.8020.97
193_A222_L0.8010.97
8_L63_C0.7990.97
187_V216_V0.7990.97
43_I91_G0.7980.96
290_K334_R0.7950.96
146_S159_V0.7940.96
190_V250_L0.7930.96
140_L245_M0.7920.96
73_A281_H0.7920.96
322_A326_V0.7920.96
188_T221_M0.7910.96
53_N86_L0.7890.96
267_A277_F0.7880.96
302_A305_L0.7840.96
43_I71_L0.7810.96
21_A25_L0.7790.96
275_H309_S0.7780.96
104_N280_V0.7680.96
48_I54_P0.7640.95
123_E235_N0.7560.95
42_L46_A0.7550.95
223_V227_A0.7530.95
20_S353_K0.7530.95
19_E346_Q0.7530.95
162_L245_M0.7520.95
45_G49_D0.7500.95
112_E324_N0.7500.95
36_F302_A0.7480.95
205_V209_V0.7470.95
230_L254_A0.7470.95
173_I218_L0.7440.95
321_D324_N0.7430.95
101_L276_L0.7430.95
108_V258_T0.7420.95
175_E179_M0.7410.95
110_V128_V0.7400.95
45_G76_G0.7400.95
166_R201_L0.7360.94
19_E40_Y0.7360.94
157_E165_K0.7310.94
176_G241_V0.7280.94
207_E218_L0.7270.94
129_D238_D0.7260.94
265_P304_M0.7250.94
41_G58_E0.7240.94
182_A311_G0.7240.94
226_A240_V0.7230.94
346_Q350_E0.7220.94
132_I250_L0.7190.94
223_V243_E0.7160.94
60_V64_K0.7160.94
209_V213_F0.7160.94
115_S126_D0.7140.94
75_G283_S0.7130.94
138_G168_E0.7130.94
64_K274_L0.7070.93
25_L306_L0.7070.93
151_N154_G0.7040.93
286_D293_A0.7030.93
151_N155_E0.7020.93
70_L298_A0.7010.93
133_V172_V0.7010.93
45_G75_G0.7000.93
265_P300_L0.6960.93
162_L246_F0.6940.93
108_V130_F0.6930.93
284_A287_I0.6930.93
350_E353_K0.6930.93
314_E317_K0.6920.92
226_A237_F0.6880.92
73_A298_A0.6870.92
175_E271_S0.6850.92
130_F257_L0.6850.92
177_F218_L0.6840.92
302_A306_L0.6820.92
225_N261_L0.6810.92
178_K212_E0.6800.92
48_I86_L0.6750.92
20_S299_I0.6740.91
122_K127_N0.6710.91
346_Q349_T0.6700.91
145_P159_V0.6700.91
158_A198_S0.6680.91
62_A65_N0.6680.91
203_R207_E0.6680.91
69_I276_L0.6650.91
316_A320_E0.6630.91
191_D222_L0.6620.91
53_N58_E0.6600.91
25_L38_F0.6580.90
18_L21_A0.6570.90
267_A304_M0.6560.90
5_I305_L0.6540.90
170_E211_Q0.6540.90
326_V348_I0.6530.90
86_L90_K0.6520.90
90_K94_S0.6510.90
172_V241_V0.6500.90
46_A49_D0.6480.90
110_V115_S0.6470.90
324_N328_A0.6470.90
91_G94_S0.6460.90
260_S297_A0.6460.90
131_V239_V0.6440.90
169_I205_V0.6430.89
57_E94_S0.6400.89
156_Q204_E0.6380.89
43_I55_L0.6370.89
299_I319_V0.6360.89
47_A55_L0.6350.89
332_R336_L0.6340.89
190_V242_T0.6300.89
210_A214_P0.6300.89
263_M336_L0.6270.88
229_Q232_Y0.6270.88
156_Q167_T0.6260.88
55_L95_I0.6240.88
5_I36_F0.6220.88
280_V297_A0.6220.88
147_K158_A0.6210.88
115_S121_K0.6210.88
331_K355_A0.6200.88
160_D200_R0.6180.88
132_I240_V0.6170.87
100_D270_S0.6150.87
129_D184_K0.6150.87
28_V33_N0.6140.87
210_A218_L0.6130.87
18_L22_T0.6110.87
111_F300_L0.6110.87
54_P86_L0.6100.87
131_V183_R0.6100.87
185_G215_D0.6100.87
333_T336_L0.6090.87
169_I243_E0.6080.87
189_S202_W0.6070.87
192_K224_D0.6070.87
42_L58_E0.6060.87
25_L319_V0.6050.86
23_D26_K0.6050.86
331_K340_E0.6030.86
72_G298_A0.6030.86
159_V163_F0.6000.86
231_I257_L0.6000.86
265_P297_A0.5990.86
115_S122_K0.5980.86
45_G79_W0.5920.85
144_Q163_F0.5920.85
36_F312_L0.5920.85
306_L319_V0.5900.85
47_A50_E0.5890.85
336_L340_E0.5890.85
100_D167_T0.5870.85
59_T91_G0.5870.85
100_D138_G0.5850.85
28_V318_A0.5840.84
318_A322_A0.5830.84
310_F313_E0.5800.84
293_A334_R0.5790.84
63_C66_A0.5790.84
299_I349_T0.5790.84
274_L277_F0.5770.84
199_S243_E0.5760.84
225_N236_Q0.5730.83
5_I38_F0.5730.83
154_G157_E0.5720.83
141_Y278_E0.5710.83
8_L41_G0.5700.83
175_E178_K0.5640.82
76_G90_K0.5630.82
168_E171_R0.5630.82
147_K197_E0.5600.82
84_S90_K0.5590.82
63_C95_I0.5580.82
314_E318_A0.5580.82
84_S87_R0.5580.82
180_A187_V0.5560.82
49_D79_W0.5540.81
54_P58_E0.5540.81
36_F315_E0.5520.81
84_S200_R0.5480.81
128_V235_N0.5480.81
270_S275_H0.5470.80
19_E22_T0.5450.80
306_L316_A0.5420.80
269_L275_H0.5370.79
144_Q161_T0.5370.79
123_E127_N0.5370.79
260_S263_M0.5360.79
243_E246_F0.5340.79
131_V184_K0.5340.79
157_E166_R0.5310.78
40_Y46_A0.5300.78
133_V176_G0.5300.78
331_K354_A0.5280.78
173_I205_V0.5270.78
55_L59_T0.5260.78
225_N237_F0.5260.78
16_E291_G0.5250.78
25_L28_V0.5240.78
55_L94_S0.5230.77
71_L95_I0.5220.77
320_E324_N0.5210.77
291_G350_E0.5200.77
347_A351_E0.5200.77
226_A250_L0.5190.77
181_A214_P0.5180.77
287_I290_K0.5180.77
318_A353_K0.5170.77
99_L276_L0.5170.77
204_E211_Q0.5170.77
13_I17_V0.5150.76
41_G56_P0.5150.76
282_G285_P0.5140.76
151_N156_Q0.5130.76
15_P46_A0.5130.76
18_L70_L0.5110.76
154_G165_K0.5100.76
63_C99_L0.5090.76
97_K143_G0.5090.76
44_G47_A0.5090.76
145_P162_L0.5060.75
178_K209_V0.5060.75
115_S336_L0.5060.75
185_G216_V0.5050.75
317_K324_N0.5050.75
179_M182_A0.5050.75
5_I68_A0.5040.75
43_I59_T0.5040.75
102_F175_E0.5040.75
74_V283_S0.5030.75
248_D252_D0.5030.75
21_A24_V0.5020.75
6_A39_E0.5000.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3u1hA 2 0.9616 100 0.028 Contact Map
3r8wA 2 0.9671 100 0.03 Contact Map
2y3zA 2 0.9589 100 0.035 Contact Map
3uduA 3 0.9616 100 0.035 Contact Map
1a05A 2 0.9644 100 0.038 Contact Map
1cnzA 2 0.9753 100 0.043 Contact Map
3vmkA 2 0.9726 100 0.048 Contact Map
3flkA 3 0.9562 100 0.049 Contact Map
4xxvA 2 0.9589 100 0.05 Contact Map
1vlcA 2 0.9644 100 0.052 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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