GREMLIN Database
GUB - Beta-glucanase
UniProt: P04957 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 242 (215)
Sequences: 3916 (2991)
Seq/√Len: 204.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
173_H189_D4.9391.00
185_K192_L3.8771.00
105_V119_F3.6451.00
192_L195_T3.3051.00
102_L176_A3.0311.00
187_Y192_L3.0211.00
68_S78_A3.0191.00
42_N45_F3.0041.00
106_R235_D2.9081.00
67_V90_G2.7941.00
76_R233_H2.6771.00
107_M119_F2.6681.00
78_A231_Y2.6201.00
74_E233_H2.5311.00
148_F193_K2.4301.00
76_R231_Y2.4281.00
104_E176_A2.4201.00
185_K195_T2.3371.00
102_L178_D2.3241.00
37_P238_R2.2591.00
177_F184_I2.2471.00
108_K233_H2.2351.00
172_Y233_H2.1711.00
48_K67_V2.1561.00
107_M232_A2.0351.00
121_T205_G2.0171.00
148_F186_W2.0091.00
47_Q93_R1.9881.00
183_S195_T1.9861.00
176_A187_Y1.9341.00
103_Y207_I1.9271.00
70_T76_R1.9261.00
106_R174_T1.9141.00
67_V92_N1.9091.00
66_N88_D1.8651.00
99_G204_P1.7851.00
79_L211_L1.7481.00
209_M237_V1.6941.00
119_F209_M1.6831.00
98_Y239_Y1.6091.00
224_Y228_N1.5871.00
32_G35_F1.5811.00
178_D185_K1.5791.00
47_Q97_T1.5711.00
40_G69_M1.4991.00
133_E137_E1.4951.00
106_R236_W1.4931.00
177_F186_W1.4821.00
35_F238_R1.4711.00
74_E172_Y1.4521.00
104_E174_T1.4441.00
70_T74_E1.4341.00
211_L232_A1.4091.00
80_T88_D1.3921.00
115_I230_L1.3901.00
160_K163_D1.3611.00
49_A93_R1.3591.00
121_T136_I1.3571.00
33_S241_K1.3421.00
104_E236_W1.3111.00
82_P88_D1.2901.00
39_N235_D1.2841.00
41_Y92_N1.2721.00
56_N60_C1.2641.00
105_V209_M1.2591.00
32_G240_T1.2421.00
68_S76_R1.2391.00
45_F98_Y1.2121.00
103_Y136_I1.2031.00
174_T190_G1.2021.00
183_S197_T1.1841.00
123_T131_W1.1791.00
109_P138_F1.1701.00
71_S74_E1.1651.00
100_Y181_P1.1571.00
41_Y48_K1.1391.00
115_I213_N1.1371.00
108_K171_A1.1311.00
115_I211_L1.1221.00
36_D45_F1.1081.00
123_T205_G1.1081.00
110_A117_S1.0951.00
122_Y208_M1.0941.00
139_L147_Q1.0821.00
36_D237_V1.0661.00
129_T132_D1.0571.00
103_Y121_T1.0531.00
142_D145_K1.0521.00
118_S137_E1.0511.00
146_V188_V1.0481.00
77_L92_N1.0451.00
132_D205_G1.0421.00
79_L213_N1.0401.00
168_A175_Y1.0341.00
58_F89_C1.0291.00
81_S87_F1.0191.00
207_I237_V1.0101.00
116_V210_N1.0091.00
30_T102_L1.0091.00
128_G132_D1.0071.00
31_G241_K1.0071.00
66_N80_T1.0031.00
120_F137_E1.0031.00
87_F229_P0.9991.00
118_S133_E0.9951.00
116_V120_F0.9941.00
161_I164_L0.9931.00
153_N157_N0.9811.00
187_Y190_G0.9781.00
57_M224_Y0.9711.00
108_K172_Y0.9671.00
225_N228_N0.9671.00
126_T131_W0.9590.99
178_D187_Y0.9390.99
47_Q96_Q0.9360.99
74_E235_D0.9300.99
175_Y188_V0.9280.99
121_T177_F0.9180.99
180_Q185_K0.9180.99
116_V212_W0.9140.99
168_A188_V0.9140.99
167_D170_N0.9130.99
106_R172_Y0.9100.99
124_G204_P0.8970.99
121_T207_I0.8960.99
49_A52_Y0.8830.99
174_T236_W0.8830.99
159_E162_V0.8760.99
99_G203_T0.8610.99
162_V165_G0.8550.99
48_K90_G0.8510.99
94_S207_I0.8460.99
130_P153_N0.8450.99
134_I150_Y0.8430.99
209_M234_Y0.8410.99
119_F207_I0.8340.99
140_G143_T0.8340.99
155_A158_H0.8310.98
54_N57_M0.8290.98
93_R206_K0.8250.98
104_E238_R0.8240.98
54_N58_F0.8210.98
120_F133_E0.8170.98
94_S98_Y0.8160.98
127_D203_T0.8140.98
34_F45_F0.8000.98
180_Q183_S0.8000.98
55_G219_E0.7930.98
65_N80_T0.7910.98
120_F208_M0.7880.98
134_I184_I0.7870.98
92_N209_M0.7810.98
125_P203_T0.7810.98
60_C212_W0.7770.98
124_G206_K0.7750.98
47_Q95_V0.7700.98
141_K220_W0.7700.98
198_N202_T0.7610.97
125_P131_W0.7590.97
96_Q124_G0.7580.97
50_D53_S0.7570.97
111_K169_A0.7570.97
176_A190_G0.7530.97
23_T26_A0.7460.97
36_D46_W0.7450.97
102_L187_Y0.7440.97
125_P204_P0.7420.97
159_E165_G0.7410.97
103_Y239_Y0.7410.97
27_S30_T0.7400.97
159_E163_D0.7340.97
123_T201_P0.7320.97
153_N156_G0.7320.97
53_S91_E0.7310.97
69_M76_R0.7300.97
82_P86_K0.7300.97
119_F136_I0.7270.97
161_I165_G0.7230.96
118_S135_D0.7220.96
56_N212_W0.7200.96
220_W228_N0.7180.96
160_K165_G0.7160.96
41_Y67_V0.7160.96
49_A91_E0.7150.96
54_N122_Y0.7130.96
207_I239_Y0.7110.96
89_C212_W0.7060.96
63_R82_P0.7060.96
30_T241_K0.7050.96
220_W224_Y0.7010.96
163_D166_F0.6940.95
120_F210_N0.6930.95
103_Y119_F0.6910.95
81_S226_G0.6890.95
186_W196_A0.6840.95
139_L221_L0.6830.95
91_E132_D0.6820.95
98_Y124_G0.6770.95
213_N224_Y0.6770.95
74_E106_R0.6750.95
118_S210_N0.6720.95
61_T66_N0.6680.94
126_T206_K0.6670.94
70_T231_Y0.6630.94
152_T199_Q0.6600.94
91_E208_M0.6570.94
168_A171_A0.6570.94
218_D221_L0.6550.94
33_S103_Y0.6540.94
102_L236_W0.6540.94
134_I177_F0.6530.94
123_T126_T0.6530.94
102_L240_T0.6490.94
188_V191_Q0.6430.93
111_K170_N0.6400.93
73_G235_D0.6340.93
139_L220_W0.6340.93
122_Y126_T0.6340.93
43_S48_K0.6330.93
24_A27_S0.6290.92
95_V127_D0.6260.92
117_S212_W0.6260.92
59_N62_W0.6250.92
100_Y179_W0.6250.92
65_N82_P0.6250.92
109_P117_S0.6230.92
138_F141_K0.6200.92
107_M175_Y0.6180.92
58_F62_W0.6170.92
103_Y177_F0.6150.92
102_L190_G0.6090.91
33_S239_Y0.6050.91
75_M92_N0.6040.91
32_G238_R0.6020.91
152_T200_I0.6010.91
66_N82_P0.6010.91
67_V77_L0.6010.91
34_F240_T0.5990.91
120_F212_W0.5980.90
199_Q202_T0.5950.90
122_Y206_K0.5950.90
37_P236_W0.5920.90
81_S86_K0.5910.90
150_Y196_A0.5900.90
218_D222_G0.5890.90
60_C89_C0.5890.90
113_T221_L0.5890.90
131_W203_T0.5870.90
77_L211_L0.5850.90
79_L231_Y0.5850.90
41_Y69_M0.5780.89
87_F114_G0.5750.89
68_S231_Y0.5750.89
186_W193_K0.5730.89
107_M234_Y0.5730.89
108_K170_N0.5720.88
162_V166_F0.5720.88
104_E187_Y0.5710.88
139_L142_D0.5690.88
60_C63_R0.5690.88
70_T233_H0.5690.88
209_M232_A0.5680.88
102_L174_T0.5680.88
100_Y203_T0.5660.88
58_F61_T0.5640.88
147_Q220_W0.5630.88
75_M209_M0.5620.88
36_D238_R0.5620.88
159_E164_L0.5600.87
160_K164_L0.5590.87
113_T223_S0.5590.87
105_V236_W0.5560.87
172_Y175_Y0.5550.87
172_Y235_D0.5540.87
24_A28_A0.5540.87
43_S97_T0.5540.87
135_D151_Y0.5530.87
218_D223_S0.5530.87
219_E223_S0.5530.87
128_G131_W0.5520.87
51_G55_G0.5480.86
141_K221_L0.5470.86
153_N200_I0.5460.86
98_Y103_Y0.5450.86
51_G54_N0.5450.86
165_G168_A0.5420.86
54_N60_C0.5400.85
79_L89_C0.5390.85
223_S227_V0.5390.85
152_T156_G0.5350.85
91_E212_W0.5350.85
25_T28_A0.5340.85
182_N198_N0.5340.85
167_D171_A0.5330.85
151_Y154_G0.5320.85
63_R83_A0.5300.84
113_T141_K0.5250.84
148_F152_T0.5230.84
117_S210_N0.5210.83
112_N227_V0.5190.83
120_F135_D0.5190.83
115_I138_F0.5180.83
210_N214_G0.5170.83
221_L228_N0.5170.83
30_T33_S0.5160.83
43_S62_W0.5150.83
67_V75_M0.5120.82
166_F170_N0.5120.82
64_A90_G0.5110.82
112_N230_L0.5110.82
127_D131_W0.5100.82
64_A67_V0.5090.82
109_P146_V0.5090.82
115_I228_N0.5080.82
60_C210_N0.5070.82
132_D214_G0.5060.82
114_G214_G0.5050.82
116_V208_M0.5040.81
176_A186_W0.5010.81
123_T206_K0.5010.81
112_N140_G0.5000.81
116_V137_E0.5000.81
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3o5sA 1 0.8802 100 0.168 Contact Map
3wvjA 1 0.8843 100 0.195 Contact Map
3i4iA 1 0.876 100 0.199 Contact Map
2ayhA 1 0.8843 100 0.202 Contact Map
3ilnA 1 0.8802 100 0.219 Contact Map
1upsA 1 0.8926 100 0.224 Contact Map
4bpzA 1 0.8843 100 0.23 Contact Map
3juuA 1 0.9174 100 0.231 Contact Map
3azyA 1 0.8636 100 0.235 Contact Map
2hykA 1 0.8595 100 0.24 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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