GREMLIN Database
SUBT - Subtilisin E
UniProt: P04189 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 381 (326)
Sequences: 11464 (8646)
Seq/√Len: 478.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
202_L208_G3.6791.00
284_G305_M3.3381.00
285_A293_A3.2591.00
336_A376_V3.1651.00
100_D211_S3.1291.00
315_L321_G3.1221.00
128_T193_S3.0941.00
134_V178_I3.0501.00
336_A374_I2.7001.00
173_H328_M2.6321.00
285_A295_F2.6271.00
229_N330_T2.6161.00
295_F309_V2.5461.00
315_L323_Y2.5361.00
293_A309_V2.5351.00
130_S193_S2.5261.00
361_T375_N2.5231.00
176_G313_S2.4581.00
246_D279_S2.3161.00
254_V281_I2.3151.00
174_V229_N2.2941.00
199_V216_G2.2621.00
348_T351_Q2.1831.00
275_A282_A2.0681.00
133_K195_S2.0651.00
167_G204_S2.0581.00
133_K197_Y2.0421.00
177_T332_H2.0381.00
254_V337_A2.0381.00
136_V178_I2.0351.00
173_H313_S1.9801.00
258_A330_T1.9491.00
239_T243_T1.9491.00
176_G314_T1.9351.00
359_T379_A1.8771.00
353_R357_E1.8651.00
330_T334_A1.8541.00
166_D169_S1.8491.00
199_V219_W1.8341.00
180_A314_T1.8081.00
179_A196_L1.8001.00
81_D84_A1.7941.00
137_I201_V1.7761.00
287_N309_V1.7661.00
221_I248_A1.7431.00
284_G302_L1.7411.00
217_I248_A1.7401.00
292_R305_M1.6871.00
135_A225_M1.6771.00
285_A308_G1.6761.00
134_V338_A1.6481.00
256_A330_T1.6481.00
135_A220_A1.6471.00
344_H347_W1.6361.00
197_Y225_M1.6251.00
285_A309_V1.6181.00
113_G332_H1.6181.00
103_A210_Y1.6151.00
286_V305_M1.6141.00
354_D358_S1.5921.00
337_A352_V1.5841.00
226_D341_L1.5731.00
177_T328_M1.5721.00
263_G292_R1.5711.00
219_W223_N1.5581.00
257_A280_T1.5571.00
323_Y328_M1.5561.00
174_V331_P1.5531.00
256_A337_A1.5311.00
209_Q212_W1.5161.00
304_V374_I1.5121.00
229_N334_A1.5111.00
142_D316_P1.5081.00
246_D250_S1.5061.00
98_E238_S1.5051.00
136_V174_V1.4971.00
142_D317_G1.4971.00
344_H355_R1.4931.00
242_K246_D1.4931.00
290_N373_L1.4911.00
128_T342_S1.4901.00
248_A255_V1.4891.00
132_V341_L1.4731.00
218_E222_S1.4631.00
333_V374_I1.4621.00
118_K375_N1.4611.00
341_L352_V1.4601.00
179_A194_A1.4581.00
116_Q290_N1.4511.00
175_A198_A1.4391.00
292_R296_S1.4371.00
221_I253_I1.4371.00
256_A333_V1.4341.00
213_I232_L1.4191.00
347_W355_R1.4161.00
312_Q322_A1.4121.00
134_V194_A1.4061.00
340_I379_A1.4041.00
98_E240_A1.4021.00
173_H323_Y1.3651.00
259_A282_A1.3511.00
44_K95_A1.3481.00
190_V335_G1.3451.00
68_K215_N1.3371.00
247_K250_S1.3121.00
242_K276_K1.3121.00
173_H177_T1.3121.00
340_I347_W1.3101.00
55_K58_V1.2991.00
258_A329_A1.2981.00
141_I198_A1.2921.00
132_V338_A1.2911.00
155_S160_E1.2831.00
287_N291_Q1.2681.00
284_G296_S1.2661.00
227_V334_A1.2521.00
375_N378_A1.2511.00
304_V356_L1.2481.00
214_I240_A1.2371.00
309_V324_N1.2331.00
141_I196_L1.2291.00
139_S168_S1.2281.00
107_A110_V1.2081.00
201_V232_L1.1941.00
286_V373_L1.1921.00
217_I230_M1.1911.00
281_I353_R1.1831.00
246_D278_P1.1671.00
347_W351_Q1.1591.00
357_E371_K1.1551.00
201_V216_G1.1541.00
141_I175_A1.1521.00
245_V280_T1.1481.00
337_A356_L1.1391.00
155_S158_P1.1281.00
283_V304_V1.1201.00
340_I352_V1.1181.00
262_E297_S1.1141.00
59_I64_G1.1141.00
214_I244_V1.1121.00
254_V349_N1.1031.00
101_H210_Y1.1021.00
254_V352_V1.1001.00
218_E247_K1.0921.00
228_I253_I1.0901.00
125_Q377_Q1.0881.00
355_R359_T1.0871.00
191_A338_A1.0811.00
208_G212_W1.0681.00
58_V62_K1.0631.00
121_A124_S1.0531.00
118_K377_Q1.0531.00
361_T373_L1.0491.00
147_D314_T1.0451.00
106_Y258_A1.0431.00
307_P311_I1.0421.00
149_N181_L1.0411.00
292_R368_Y1.0391.00
210_Y241_L1.0391.00
85_V89_K1.0321.00
256_A334_A1.0291.00
151_R181_L1.0261.00
190_V332_H1.0231.00
139_S166_D1.0181.00
86_K89_K1.0171.00
228_I248_A1.0151.00
306_A333_V1.0141.00
145_H320_Y1.0141.00
243_T247_K1.0131.00
217_I228_I1.0101.00
121_A377_Q1.0051.00
117_I190_V1.0031.00
262_E272_G1.0011.00
141_I148_L1.0001.00
264_S272_G0.9981.00
180_A187_V0.9961.00
313_S328_M0.9931.00
105_E108_Q0.9871.00
175_A196_L0.9861.00
298_A368_Y0.9731.00
259_A302_L0.9711.00
137_I216_G0.9691.00
242_K277_Y0.9661.00
127_Y342_S0.9651.00
246_D277_Y0.9641.00
119_A123_H0.9561.00
217_I245_V0.9541.00
283_V333_V0.9471.00
136_V175_A0.9471.00
118_K121_A0.9471.00
228_I255_V0.9471.00
155_S200_K0.9451.00
143_S165_Q0.9431.00
341_L345_P0.9381.00
221_I251_S0.9371.00
363_L373_L0.9291.00
286_V290_N0.9261.00
191_A194_A0.9221.00
336_A356_L0.9171.00
273_Y276_K0.9161.00
231_S331_P0.9161.00
220_A228_I0.9161.00
310_S324_N0.9141.00
273_Y277_Y0.9131.00
122_L125_Q0.9101.00
129_G191_A0.9031.00
44_K93_S0.8991.00
142_D318_G0.8991.00
328_M331_P0.8991.00
55_K59_I0.8981.00
107_A111_P0.8921.00
199_V220_A0.8881.00
337_A340_I0.8861.00
249_V353_R0.8831.00
230_M257_A0.8791.00
74_N78_A0.8761.00
303_D357_E0.8651.00
66_V70_F0.8611.00
230_M255_V0.8601.00
172_T314_T0.8571.00
264_S298_A0.8561.00
292_R298_A0.8551.00
220_A248_A0.8521.00
54_K58_V0.8511.00
154_A157_V0.8491.00
84_A87_E0.8481.00
152_G197_Y0.8481.00
88_L94_V0.8371.00
249_V279_S0.8301.00
100_D103_A0.8291.00
229_N256_A0.8291.00
360_A372_G0.8291.00
249_V350_A0.8251.00
87_E90_K0.8251.00
131_N195_S0.8221.00
304_V360_A0.8181.00
152_G155_S0.8161.00
362_Y366_S0.8151.00
350_A354_D0.8121.00
72_Y218_E0.8081.00
227_V254_V0.8061.00
340_I344_H0.8041.00
169_S315_L0.8031.00
213_I230_M0.8021.00
80_L84_A0.8001.00
58_V61_E0.7981.00
236_T276_K0.7961.00
295_F324_N0.7931.00
142_D146_P0.7921.00
77_A211_S0.7881.00
85_V97_V0.7781.00
133_K224_N0.7771.00
129_G194_A0.7751.00
108_Q111_P0.7741.00
170_H327_S0.7711.00
219_W222_S0.7681.00
137_I230_M0.7631.00
199_V223_N0.7621.00
147_D320_Y0.7621.00
96_Y243_T0.7601.00
150_V181_L0.7601.00
201_V213_I0.7571.00
166_D200_K0.7561.00
120_P123_H0.7541.00
333_V356_L0.7531.00
304_V357_E0.7511.00
138_D170_H0.7501.00
336_A339_L0.7481.00
153_G156_F0.7481.00
261_N331_P0.7481.00
142_D315_L0.7471.00
215_N218_E0.7441.00
210_Y234_G0.7421.00
249_V349_N0.7411.00
263_G298_A0.7401.00
218_E221_I0.7391.00
282_A303_D0.7391.00
247_K251_S0.7391.00
100_D209_Q0.7381.00
293_A324_N0.7371.00
117_I123_H0.7361.00
239_T242_K0.7351.00
285_A324_N0.7321.00
169_S323_Y0.7311.00
66_V69_Q0.7311.00
341_L349_N0.7311.00
232_L257_A0.7301.00
135_A197_Y0.7281.00
137_I228_I0.7271.00
259_A284_G0.7251.00
277_Y280_T0.7231.00
361_T378_A0.7191.00
145_H176_G0.7141.00
178_I335_G0.7131.00
272_G275_A0.7101.00
133_K226_D0.7101.00
147_D183_N0.7101.00
339_L376_V0.7091.00
70_F77_A0.7081.00
307_P332_H0.7031.00
155_S164_Y0.7031.00
132_V342_S0.7021.00
281_I337_A0.7021.00
279_S350_A0.7001.00
340_I355_R0.6991.00
250_S279_S0.6911.00
347_W352_V0.6911.00
126_G129_G0.6901.00
102_I209_Q0.6881.00
144_S165_Q0.6871.00
245_V255_V0.6851.00
341_L347_W0.6801.00
254_V341_L0.6801.00
67_Q79_T0.6791.00
132_V194_A0.6791.00
281_I357_E0.6751.00
284_G292_R0.6751.00
135_A228_I0.6751.00
222_S247_K0.6711.00
82_E86_K0.6701.00
103_A213_I0.6651.00
56_K60_S0.6611.00
70_F218_E0.6601.00
354_D357_E0.6591.00
120_P124_S0.6581.00
116_Q286_V0.6581.00
227_V337_A0.6571.00
263_G297_S0.6571.00
89_K97_V0.6551.00
202_L232_L0.6551.00
156_F159_S0.6541.00
174_V328_M0.6541.00
327_S331_P0.6541.00
252_G341_L0.6521.00
220_A253_I0.6521.00
94_V97_V0.6501.00
217_I255_V0.6481.00
341_L344_H0.6461.00
221_I247_K0.6411.00
137_I199_V0.6381.00
220_A225_M0.6371.00
288_S310_S0.6371.00
145_H314_T0.6351.00
217_I241_L0.6311.00
177_T311_I0.6291.00
233_G258_A0.6281.00
281_I304_V0.6281.00
331_P334_A0.6271.00
98_E239_T0.6261.00
135_A199_V0.6261.00
59_I62_K0.6251.00
252_G349_N0.6230.99
194_A338_A0.6220.99
304_V333_V0.6210.99
246_D249_V0.6200.99
244_V248_A0.6180.99
127_Y343_K0.6170.99
257_A275_A0.6170.99
80_L88_L0.6160.99
145_H148_L0.6160.99
227_V256_A0.6150.99
132_V227_V0.6150.99
134_V227_V0.6140.99
46_T49_A0.6140.99
362_Y369_Y0.6130.99
103_A234_G0.6130.99
302_L305_M0.6100.99
156_F219_W0.6100.99
133_K225_M0.6070.99
315_L319_T0.6070.99
258_A272_G0.6050.99
363_L369_Y0.6050.99
174_V330_T0.6050.99
128_T192_P0.6050.99
240_A243_T0.6040.99
254_V353_R0.6030.99
148_L314_T0.6010.99
326_T331_P0.5980.99
110_V113_G0.5970.99
77_A100_D0.5970.99
168_S323_Y0.5960.99
249_V255_V0.5940.99
155_S159_S0.5900.99
249_V254_V0.5890.99
249_V277_Y0.5890.99
152_G198_A0.5880.99
117_I332_H0.5870.99
152_G157_V0.5850.99
70_F215_N0.5850.99
142_D165_Q0.5840.99
167_G200_K0.5840.99
70_F73_V0.5820.99
292_R369_Y0.5810.99
68_K77_A0.5810.99
197_Y223_N0.5780.99
156_F199_V0.5780.99
174_V231_S0.5780.99
56_K61_E0.5780.99
146_P318_G0.5780.99
258_A331_P0.5760.99
312_Q320_Y0.5740.99
95_A99_E0.5730.99
86_K90_K0.5720.99
349_N353_R0.5720.99
54_K57_D0.5710.99
56_K59_I0.5700.99
112_Y116_Q0.5700.99
183_N186_G0.5680.99
244_V247_K0.5680.99
114_I119_A0.5680.99
80_L85_V0.5680.99
106_Y111_P0.5660.99
146_P185_I0.5660.99
72_Y222_S0.5650.99
230_M245_V0.5650.99
115_S120_P0.5650.99
113_G177_T0.5630.99
208_G213_I0.5620.99
216_G220_A0.5610.99
136_V229_N0.5600.99
170_H261_N0.5580.99
210_Y238_S0.5560.99
344_H351_Q0.5560.99
310_S322_A0.5560.99
355_R358_S0.5550.99
68_K72_Y0.5540.99
180_A189_G0.5530.99
131_N191_A0.5520.99
237_G242_K0.5510.99
153_G157_V0.5510.99
256_A281_I0.5500.99
272_G297_S0.5500.99
143_S198_A0.5500.99
203_D207_S0.5480.99
211_S215_N0.5450.99
366_S371_K0.5450.99
243_T246_D0.5440.99
147_D180_A0.5430.99
212_W215_N0.5420.99
44_K49_A0.5420.99
227_V338_A0.5410.99
214_I241_L0.5400.99
303_D353_R0.5390.99
197_Y219_W0.5390.99
311_I332_H0.5390.99
103_A241_L0.5370.98
231_S258_A0.5370.98
294_S324_N0.5350.98
340_I359_T0.5340.98
190_V336_A0.5340.98
225_M253_I0.5340.98
233_G262_E0.5340.98
113_G116_Q0.5330.98
261_N327_S0.5310.98
364_G369_Y0.5310.98
360_A371_K0.5300.98
110_V312_Q0.5270.98
154_A160_E0.5260.98
189_G335_G0.5250.98
67_Q77_A0.5240.98
54_K59_I0.5220.98
55_K62_K0.5210.98
138_D171_G0.5200.98
129_G342_S0.5200.98
45_Q95_A0.5190.98
154_A159_S0.5190.98
78_A81_D0.5190.98
87_E91_D0.5190.98
203_D206_G0.5180.98
45_Q48_S0.5170.98
281_I356_L0.5160.98
111_P312_Q0.5150.98
202_L212_W0.5140.98
148_L151_R0.5120.98
133_K223_N0.5100.98
210_Y214_I0.5100.98
65_K79_T0.5070.98
201_V230_M0.5070.98
257_A282_A0.5060.98
59_I63_G0.5060.98
339_L342_S0.5020.98
104_H207_S0.5020.98
132_V226_D0.5020.98
177_T313_S0.5020.98
200_K203_D0.5010.98
366_S369_Y0.5010.98
105_E111_P0.5000.98
139_S169_S0.5000.98
229_N331_P0.5000.98
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3whiA 2 0.9108 100 0.126 Contact Map
3afgA 1 0.9633 100 0.206 Contact Map
3wiuA 2 0.8793 100 0.208 Contact Map
1r6vA 1 0.9606 100 0.211 Contact Map
3t41A 1 0.8871 100 0.228 Contact Map
4tr2A 2 0.9134 100 0.229 Contact Map
2p4eP 2 0.8976 100 0.242 Contact Map
1to2E 1 0.7218 100 0.248 Contact Map
1r0rE 1 0.7192 100 0.252 Contact Map
1gciA 1 0.706 100 0.259 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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