GREMLIN Database
MNMA - tRNA-specific 2-thiouridylase MnmA
UniProt: O35020 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 371 (348)
Sequences: 3673 (2125)
Seq/√Len: 113.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
303_S332_D3.8421.00
91_E96_R3.6001.00
25_L186_A3.4831.00
224_T228_E3.4681.00
221_D267_I3.4581.00
9_R33_D3.3401.00
184_K192_T3.2981.00
221_D229_V2.9211.00
132_R173_E2.8851.00
221_D232_R2.8711.00
259_V271_D2.7251.00
118_L166_K2.7191.00
287_T359_D2.7141.00
181_E185_E2.6701.00
225_M278_L2.6441.00
178_R181_E2.5861.00
262_D265_K2.5841.00
283_K328_E2.5591.00
219_P232_R2.4861.00
306_A344_V2.4231.00
29_E185_E2.2921.00
223_M267_I2.2611.00
286_A344_V2.2571.00
141_R351_E2.1961.00
320_R328_E2.1161.00
145_G153_T2.0661.00
289_I346_F2.0601.00
38_F71_Y2.0501.00
301_E320_R2.0431.00
59_D193_K2.0421.00
236_L268_L2.0401.00
283_K330_I1.9551.00
144_R307_K1.9431.00
132_R146_I1.9401.00
87_Y103_L1.9271.00
276_N336_R1.9181.00
284_I344_V1.9111.00
306_A317_V1.9061.00
262_D267_I1.9051.00
206_N209_T1.8971.00
21_S190_T1.8851.00
222_M270_V1.8831.00
252_S256_W1.8771.00
181_E184_K1.8661.00
329_V346_F1.8291.00
132_R172_G1.8191.00
254_E272_Q1.8041.00
81_Y86_Q1.7991.00
160_T163_T1.7751.00
35_I72_Y1.7751.00
157_N343_A1.7751.00
329_V344_V1.7661.00
11_V122_A1.7491.00
175_Q178_R1.7341.00
305_T316_K1.7331.00
159_L163_T1.7071.00
225_M259_V1.6881.00
316_K332_D1.6791.00
304_C317_V1.6471.00
315_H335_V1.6261.00
27_L126_A1.6211.00
343_A357_T1.6131.00
303_S316_K1.6131.00
66_Q189_A1.6111.00
260_G269_Y1.6011.00
320_R330_I1.5951.00
134_D137_G1.5841.00
303_S318_T1.5761.00
12_V23_A1.5701.00
281_S338_V1.5621.00
307_K314_D1.5601.00
14_M21_S1.5551.00
133_V140_V1.5451.00
157_N355_G1.5441.00
198_G205_R1.5401.00
36_G69_I1.5331.00
106_K158_Q1.5321.00
157_N357_T1.5261.00
149_N177_S1.5171.00
24_A69_I1.5031.00
322_T328_E1.4881.00
140_V161_E1.4791.00
236_L261_K1.4391.00
29_E186_A1.4221.00
272_Q278_L1.4161.00
134_D141_R1.4121.00
90_D93_K1.3981.00
262_D269_Y1.3961.00
90_D94_A1.3941.00
144_R314_D1.3811.00
229_V232_R1.3781.00
174_L179_V1.3741.00
318_T332_D1.3671.00
180_R195_D1.3581.00
305_T314_D1.3581.00
11_V37_I1.3561.00
111_K115_E1.3471.00
319_V346_F1.3411.00
304_C353_L1.3351.00
25_L29_E1.3251.00
182_I186_A1.3231.00
60_V71_Y1.3181.00
81_Y214_Y1.3181.00
285_T328_E1.3121.00
102_V343_A1.3121.00
223_M265_K1.3111.00
8_T123_D1.3051.00
161_E292_V1.2950.99
14_M36_G1.2930.99
244_R255_P1.2930.99
284_I331_F1.2910.99
286_A356_G1.2900.99
224_T230_K1.2850.99
143_L350_E1.2840.99
208_K234_D1.2800.99
134_D143_L1.2800.99
230_K270_V1.2660.99
22_V182_I1.2650.99
302_I319_V1.2580.99
322_T326_E1.2560.99
86_Q90_D1.2490.99
10_V32_Y1.2450.99
87_Y91_E1.2440.99
140_V165_S1.2380.99
83_K107_E1.2370.99
61_I65_N1.2260.99
78_K82_E1.2230.99
289_I327_A1.2220.99
223_M229_V1.2160.99
280_Y336_R1.2120.99
209_T213_Q1.2110.99
204_E209_T1.2080.99
132_R350_E1.2060.99
294_S297_M1.2040.99
9_R35_I1.2010.99
223_M269_Y1.2010.99
261_K268_L1.1940.99
219_P234_D1.1850.99
116_H119_S1.1850.99
28_K68_G1.1780.99
11_V35_I1.1770.99
21_S63_V1.1750.99
136_S139_K1.1730.99
143_L351_E1.1730.99
343_A355_G1.1730.99
284_I338_V1.1660.99
285_T360_D1.1640.99
282_D330_I1.1610.99
8_T124_Y1.1560.99
222_M268_L1.1550.99
249_I270_V1.1540.99
174_L182_I1.1530.99
318_T330_I1.1450.99
10_V168_M1.1370.99
321_M327_A1.1370.99
25_L182_I1.1320.99
139_K161_E1.1280.99
322_T325_G1.1220.99
249_I252_S1.1200.99
307_K310_Y1.1160.98
28_K67_I1.1130.98
331_F335_V1.1020.98
35_I122_A1.1010.98
8_T32_Y1.0910.98
253_G256_W1.0910.98
144_R305_T1.0830.98
301_E321_M1.0820.98
327_A346_F1.0810.98
10_V124_Y1.0800.98
113_F125_L1.0790.98
135_R140_V1.0670.98
289_I356_G1.0540.98
178_R185_E1.0530.98
147_D175_Q1.0490.98
276_N280_Y1.0450.98
57_Y71_Y1.0400.98
124_Y168_M1.0350.97
96_R287_T1.0160.97
212_S238_Y1.0130.97
225_M271_D0.9940.97
348_D351_E0.9900.97
51_C56_D0.9890.97
132_R143_L0.9830.97
301_E318_T0.9780.96
145_G171_I0.9780.96
66_Q187_E0.9760.96
146_I173_E0.9710.96
57_Y73_A0.9690.96
222_M230_K0.9660.96
91_E94_A0.9650.96
208_K238_Y0.9650.96
13_G113_F0.9640.96
224_T256_W0.9630.96
285_T326_E0.9620.96
38_F57_Y0.9580.96
162_D165_S0.9510.96
171_I179_V0.9480.96
61_I71_Y0.9470.96
182_I185_E0.9440.96
208_K211_L0.9370.95
285_T322_T0.9360.95
304_C346_F0.9330.95
24_A36_G0.9320.95
112_A115_E0.9310.95
145_G148_E0.9260.95
145_G172_G0.9250.95
118_L162_D0.9240.95
272_Q277_P0.9200.95
114_L118_L0.9200.95
144_R153_T0.9100.95
153_T310_Y0.9070.95
245_H255_P0.9020.94
284_I329_V0.9020.94
203_G209_T0.9000.94
89_L215_L0.9000.94
157_N345_V0.8980.94
102_V157_N0.8920.94
286_A346_F0.8750.94
146_I175_Q0.8750.94
308_F312_Q0.8740.93
22_V183_A0.8720.93
58_E61_I0.8720.93
284_I306_A0.8710.93
155_F311_R0.8700.93
22_V179_V0.8690.93
35_I70_P0.8660.93
89_L214_Y0.8620.93
82_E86_Q0.8610.93
312_Q315_H0.8560.93
148_E175_Q0.8530.93
326_E359_D0.8460.92
80_Y101_D0.8450.92
14_M64_C0.8440.92
159_L292_V0.8430.92
296_I299_G0.8380.92
304_C319_V0.8340.92
62_R65_N0.8230.91
291_W296_I0.8210.91
43_D77_E0.8200.91
135_R138_G0.8190.91
160_T165_S0.8180.91
125_L167_V0.8180.91
220_G233_H0.8150.91
142_M352_C0.8150.91
139_K294_S0.8140.91
284_I308_F0.8070.90
110_F167_V0.8060.90
88_F92_Y0.8030.90
132_R171_I0.7980.90
210_F214_Y0.7950.90
230_K249_I0.7920.90
13_G34_V0.7890.89
236_L263_L0.7880.89
306_A331_F0.7850.89
59_D190_T0.7830.89
297_M321_M0.7830.89
131_A153_T0.7820.89
190_T193_K0.7790.89
25_L28_K0.7780.89
323_G326_E0.7780.89
256_W270_V0.7760.89
302_I348_D0.7750.89
163_T167_V0.7730.88
107_E111_K0.7720.88
259_V278_L0.7700.88
308_F335_V0.7680.88
40_K75_N0.7650.88
37_I122_A0.7650.88
218_Q266_N0.7650.88
158_Q343_A0.7650.88
9_R121_G0.7640.88
139_K295_D0.7640.88
288_N325_G0.7620.88
18_V183_A0.7620.88
180_R190_T0.7560.87
79_Q112_A0.7560.87
42_W85_F0.7540.87
252_S270_V0.7540.87
26_L174_L0.7510.87
249_I256_W0.7470.87
308_F331_F0.7460.87
40_K77_E0.7440.87
211_L215_L0.7430.86
41_N80_Y0.7410.86
133_V164_L0.7400.86
282_D334_Q0.7390.86
59_D180_R0.7360.86
25_L67_I0.7350.86
345_V352_C0.7340.86
203_G206_N0.7340.86
291_W354_G0.7310.86
94_A287_T0.7280.85
60_V64_C0.7250.85
26_L182_I0.7240.85
127_T199_I0.7240.85
327_A356_G0.7220.85
23_A171_I0.7210.85
85_F214_Y0.7170.84
346_F354_G0.7170.84
74_V116_H0.7110.84
159_L164_L0.7100.84
102_V158_Q0.7090.84
258_A268_L0.7060.84
297_M302_I0.7050.83
37_I113_F0.7020.83
72_Y120_L0.7000.83
146_I172_G0.6990.83
222_M236_L0.6990.83
317_V346_F0.6960.83
286_A327_A0.6960.83
11_V117_A0.6950.83
286_A306_A0.6950.83
14_M63_V0.6880.82
335_V338_V0.6860.82
99_N102_V0.6850.82
26_L173_E0.6850.82
280_Y337_A0.6850.82
234_D239_Y0.6850.82
153_T352_C0.6840.82
27_L168_M0.6810.81
42_W210_F0.6800.81
116_H120_L0.6760.81
10_V34_V0.6760.81
44_D77_E0.6740.81
80_Y203_G0.6740.81
84_V199_I0.6740.81
223_M230_K0.6730.81
180_R193_K0.6720.80
39_M76_F0.6700.80
22_V186_A0.6700.80
241_I261_K0.6670.80
171_I175_Q0.6650.80
101_D198_G0.6650.80
250_G253_G0.6650.80
291_W321_M0.6640.80
62_R66_Q0.6640.80
41_N198_G0.6640.80
250_G255_P0.6630.80
319_V329_V0.6630.80
310_Y314_D0.6620.79
247_L268_L0.6620.79
286_A289_I0.6570.79
319_V327_A0.6570.79
80_Y85_F0.6560.79
293_R297_M0.6550.79
312_Q335_V0.6550.79
225_M269_Y0.6540.79
144_R148_E0.6530.78
315_H333_E0.6520.78
161_E165_S0.6520.78
18_V191_A0.6520.78
60_V63_V0.6510.78
149_N311_R0.6490.78
145_G310_Y0.6480.78
41_N101_D0.6470.78
37_I74_V0.6470.78
97_T240_T0.6460.78
27_L30_Q0.6460.78
221_D262_D0.6460.78
135_R141_R0.6440.78
13_G125_L0.6430.77
144_R350_E0.6420.77
85_F89_L0.6420.77
132_R145_G0.6420.77
256_W272_Q0.6400.77
161_E164_L0.6400.77
271_D278_L0.6390.77
222_M252_S0.6380.77
27_L34_V0.6380.77
186_A189_A0.6360.77
303_S348_D0.6360.77
239_Y247_L0.6350.77
291_W327_A0.6340.76
10_V27_L0.6330.76
18_V195_D0.6330.76
81_Y85_F0.6310.76
38_F60_V0.6310.76
133_V169_F0.6300.76
227_G269_Y0.6280.76
85_F210_F0.6270.76
244_R257_F0.6240.75
260_G265_K0.6220.75
62_R189_A0.6210.75
79_Q108_I0.6200.75
137_G141_R0.6200.75
307_K311_R0.6200.75
10_V126_A0.6200.75
56_D59_D0.6190.75
321_M325_G0.6190.75
208_K237_M0.6180.75
296_I321_M0.6180.75
133_V167_V0.6170.74
117_A120_L0.6150.74
126_A168_M0.6140.74
236_L266_N0.6140.74
158_Q355_G0.6130.74
147_D155_F0.6120.74
209_T212_S0.6120.74
299_G321_M0.6100.74
202_I210_F0.6100.74
114_L163_T0.6090.74
41_N311_R0.6090.74
297_M301_E0.6080.73
41_N76_F0.6080.73
131_A159_L0.6070.73
24_A67_I0.6070.73
260_G281_S0.6070.73
26_L30_Q0.6030.73
56_D80_Y0.6030.73
15_S19_D0.6030.73
65_N68_G0.6020.73
114_L125_L0.6000.72
99_N310_Y0.6000.72
220_G235_G0.5970.72
61_I72_Y0.5960.72
55_E58_E0.5950.72
212_S217_A0.5950.72
58_E62_R0.5950.72
282_D333_E0.5950.72
291_W356_G0.5940.72
39_M79_Q0.5940.72
117_A125_L0.5920.71
306_A346_F0.5920.71
64_C71_Y0.5860.71
18_V188_L0.5850.71
212_S237_M0.5840.70
115_E118_L0.5830.70
103_L106_K0.5820.70
101_D203_G0.5810.70
229_V267_I0.5810.70
180_R191_A0.5810.70
30_Q173_E0.5780.70
79_Q83_K0.5780.70
198_G207_F0.5780.70
256_W261_K0.5770.70
155_F169_F0.5730.69
134_D139_K0.5720.69
261_K308_F0.5710.69
276_N279_L0.5700.69
316_K333_E0.5700.69
93_K211_L0.5690.68
13_G27_L0.5680.68
9_R122_A0.5680.68
18_V190_T0.5670.68
39_M202_I0.5670.68
64_C69_I0.5670.68
291_W297_M0.5660.68
57_Y61_I0.5660.68
292_V354_G0.5640.68
317_V329_V0.5630.68
197_T205_R0.5600.67
21_S36_G0.5580.67
14_M24_A0.5580.67
33_D70_P0.5570.67
250_G256_W0.5570.67
134_D138_G0.5560.67
103_L107_E0.5540.66
141_R350_E0.5540.66
140_V159_L0.5530.66
142_M292_V0.5530.66
241_I338_V0.5510.66
133_V159_L0.5500.66
189_A192_T0.5490.66
149_N175_Q0.5490.66
344_V358_I0.5470.65
305_T348_D0.5470.65
102_V107_E0.5450.65
147_D177_S0.5450.65
161_E294_S0.5440.65
211_L237_M0.5430.65
43_D81_Y0.5410.65
225_M260_G0.5410.65
306_A329_V0.5400.64
131_A167_V0.5400.64
259_V270_V0.5390.64
281_S337_A0.5380.64
334_Q360_D0.5370.64
136_S141_R0.5360.64
254_E276_N0.5360.64
199_I202_I0.5360.64
148_E153_T0.5350.64
160_T167_V0.5350.64
161_E293_R0.5340.64
109_K155_F0.5340.64
250_G254_E0.5310.63
315_H338_V0.5310.63
304_C329_V0.5300.63
224_T252_S0.5300.63
17_G20_S0.5290.63
118_L123_D0.5290.63
56_D193_K0.5290.63
297_M327_A0.5260.62
79_Q116_H0.5240.62
23_A26_L0.5230.62
35_I71_Y0.5230.62
321_M324_E0.5220.62
115_E119_S0.5220.62
35_I120_L0.5210.62
195_D311_R0.5200.62
184_K191_A0.5200.62
146_I178_R0.5190.61
15_S20_S0.5190.61
261_K312_Q0.5180.61
14_M65_N0.5180.61
305_T349_G0.5160.61
90_D215_L0.5150.61
217_A234_D0.5150.61
216_P237_M0.5150.61
231_G266_N0.5130.61
36_G92_Y0.5110.60
44_D47_E0.5110.60
26_L171_I0.5080.60
271_D276_N0.5070.60
34_V69_I0.5070.60
96_R340_P0.5060.60
42_W203_G0.5050.59
287_T333_E0.5050.59
308_F338_V0.5050.59
217_A237_M0.5010.59
202_I207_F0.5000.59
39_M337_A0.5000.59
22_V26_L0.5000.59
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2hmaA 2 0.9757 100 0.273 Contact Map
2derA 1 0.9542 100 0.276 Contact Map
4nzpA 4 0.8464 100 0.599 Contact Map
4xfjA 4 0.8706 100 0.642 Contact Map
1korA 4 0.8544 100 0.69 Contact Map
1k92A 4 0.8814 100 0.73 Contact Map
3k32A 2 0.5148 100 0.758 Contact Map
2nz2A 4 0.8356 100 0.767 Contact Map
1vl2A 4 0.8625 100 0.777 Contact Map
4kr6A 2 0.5741 100 0.789 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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