GREMLIN Database
PURU - Formyltetrahydrofolate deformylase
UniProt: O34990 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 300 (280)
Sequences: 2257 (1202)
Seq/√Len: 71.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
144_R148_E5.3501.00
238_Y284_H5.2761.00
170_K173_E4.9521.00
141_E144_R4.6251.00
136_V147_V3.9691.00
250_E255_R3.8361.00
157_M167_V3.7501.00
219_N222_K3.3751.00
40_L45_A3.3051.00
106_V131_A3.2871.00
160_N163_I3.2351.00
39_F84_F3.1351.00
104_K287_D2.8031.00
131_A285_L2.7531.00
194_P241_N2.6641.00
107_A135_V2.5831.00
252_D276_V2.5371.00
105_R132_E2.5311.00
77_K81_Q2.4871.00
110_V138_S2.4741.00
172_L199_A2.4621.00
260_D268_I2.3021.00
173_E177_Q2.2701.00
134_A180_V2.2531.00
103_L132_E2.1751.00
176_E199_A2.1611.00
200_H204_I2.0971.00
242_D245_E2.0661.00
28_D46_N2.0441.00
109_F171_Q2.0191.00
119_E123_E1.9701.00
105_R179_D1.9401.00
120_L281_V1.9261.00
43_H83_A1.9061.00
168_E193_T1.8961.00
135_V178_Y1.8191.00
107_A180_V1.8011.00
114_L143_A1.7961.00
98_T287_D1.7841.00
28_D55_T1.7811.00
137_I171_Q1.7781.00
236_S280_A1.7321.00
185_L239_V1.7181.00
182_V281_V1.6881.00
272_I276_V1.6751.00
145_E148_E1.6511.00
163_I166_E1.6430.99
175_L183_I1.6310.99
39_F83_A1.6240.99
141_E156_Y1.6240.99
201_P204_I1.6210.99
123_E278_A1.6010.99
25_S94_T1.5910.99
76_K79_S1.5660.99
112_K142_E1.5300.99
49_E67_E1.4970.99
202_N291_V1.4930.99
254_E272_I1.4670.99
19_K67_E1.4670.99
35_A91_F1.4650.99
172_L200_H1.4620.99
36_V64_L1.4610.99
292_H297_I1.4560.99
106_V182_V1.4530.99
35_A90_K1.4480.99
32_I91_F1.4420.99
262_A266_K1.4090.98
262_A265_L1.4070.98
34_S38_A1.3980.98
164_R193_T1.3780.98
38_A42_E1.3760.98
155_H178_Y1.3710.98
165_A195_D1.3580.98
85_A89_E1.3470.98
105_R134_A1.3240.98
135_V180_V1.3230.98
257_D260_D1.3060.97
195_D198_S1.2800.97
21_R287_D1.2780.97
222_K266_K1.2570.97
159_A164_R1.2400.96
22_L97_L1.2310.96
77_K99_L1.2300.96
165_A169_K1.2250.96
114_L122_W1.2190.96
169_K173_E1.2140.96
107_A175_L1.2100.96
88_A95_W1.2070.96
164_R190_Q1.1920.96
159_A167_V1.1920.96
119_E271_T1.1860.95
118_H122_W1.1770.95
37_S50_S1.1680.95
103_L130_M1.1680.95
216_I245_E1.1630.95
128_N270_R1.1600.95
282_K286_E1.1600.95
157_M170_K1.1490.95
194_P198_S1.1470.95
146_L150_L1.1360.94
141_E148_E1.1300.94
29_Q90_K1.1190.94
166_E170_K1.1120.94
223_R245_E1.1120.94
261_N264_A1.1040.93
52_Q66_I1.1040.93
131_A134_A1.1030.93
119_E278_A1.0900.93
169_K195_D1.0860.93
107_A137_I1.0850.93
222_K226_E1.0830.93
23_L96_S1.0790.93
159_A163_I1.0760.92
109_F183_I1.0690.92
155_H174_L1.0680.92
157_M174_L1.0650.92
124_W133_I1.0590.92
45_A70_C1.0590.92
84_F88_A1.0530.92
36_V93_M1.0500.92
197_V204_I1.0420.91
160_N166_E1.0350.91
79_S82_A1.0340.91
198_S201_P1.0310.91
21_R100_A1.0260.91
104_K181_D1.0240.90
34_S41_F1.0170.90
84_F97_L1.0080.90
20_G68_F1.0050.90
184_V277_L1.0030.90
106_V134_A1.0000.89
205_I249_I0.9970.89
22_L68_F0.9960.89
145_E151_N0.9950.89
252_D279_R0.9880.89
172_L195_D0.9760.88
86_S90_K0.9720.88
82_A85_A0.9720.88
39_F43_H0.9680.88
250_E279_R0.9660.88
114_L118_H0.9570.87
78_S81_Q0.9490.87
231_L255_R0.9480.87
50_S66_I0.9410.86
61_R292_H0.9410.86
138_S188_Y0.9350.86
123_E271_T0.9310.86
284_H289_V0.9280.86
171_Q196_F0.9260.85
32_I52_Q0.9250.85
118_H146_L0.9240.85
46_N69_D0.9230.85
111_S138_S0.9220.85
183_I200_H0.9120.85
109_F137_I0.9110.84
84_F95_W0.9050.84
29_Q92_D0.9040.84
104_K285_L0.9020.84
46_N52_Q0.9010.84
58_E226_E0.9010.84
86_S89_E0.9010.84
107_A174_L0.8980.84
40_L68_F0.8880.83
267_N282_K0.8870.83
172_L176_E0.8790.82
159_A188_Y0.8700.82
61_R94_T0.8670.81
264_A267_N0.8630.81
81_Q95_W0.8600.81
124_W132_E0.8580.81
48_I69_D0.8470.80
123_E128_N0.8440.80
134_A178_Y0.8370.79
252_D255_R0.8320.79
114_L125_Q0.8320.79
105_R180_V0.8310.79
231_L250_E0.8300.78
147_V152_I0.8300.78
28_D50_S0.8290.78
32_I46_N0.8260.78
32_I36_V0.8220.78
192_L196_F0.8180.77
219_N266_K0.8170.77
108_I133_I0.8170.77
27_P92_D0.8160.77
290_I299_F0.8140.77
83_A86_S0.8120.77
99_L102_E0.8070.77
130_M285_L0.8060.76
106_V285_L0.8030.76
29_Q35_A0.8020.76
39_F87_V0.7960.76
271_T275_S0.7930.75
210_S239_V0.7910.75
167_V171_Q0.7900.75
143_A154_F0.7900.75
135_V174_L0.7900.75
30_P41_F0.7860.75
37_S41_F0.7850.75
49_E259_R0.7830.74
197_V202_N0.7810.74
108_I120_L0.7810.74
79_S83_A0.7800.74
225_Y295_K0.7780.74
19_K48_I0.7780.74
110_V117_L0.7740.73
118_H274_R0.7730.73
46_N204_I0.7720.73
254_E268_I0.7710.73
30_P46_N0.7700.73
198_S241_N0.7700.73
173_E176_E0.7680.73
197_V241_N0.7670.73
25_S61_R0.7620.72
56_D59_G0.7560.72
106_V181_D0.7510.71
136_V152_I0.7450.71
122_W150_L0.7410.70
24_V36_V0.7390.70
113_E223_R0.7320.69
193_T280_A0.7300.69
135_V155_H0.7260.69
157_M171_Q0.7260.69
33_V37_S0.7240.68
28_D32_I0.7230.68
171_Q175_L0.7230.68
114_L128_N0.7220.68
168_E280_A0.7190.68
134_A137_I0.7150.67
159_A193_T0.7150.67
40_L66_I0.7130.67
77_K97_L0.7070.67
19_K69_D0.7040.66
42_E83_A0.7040.66
124_W152_I0.7030.66
168_E236_S0.7030.66
26_C93_M0.6990.66
197_V203_R0.6950.65
81_Q99_L0.6950.65
98_T102_E0.6950.65
148_E151_N0.6910.65
52_Q64_L0.6910.65
113_E266_K0.6900.65
33_V66_I0.6900.65
24_V55_T0.6860.64
77_K101_S0.6850.64
182_V285_L0.6850.64
209_H234_A0.6810.64
30_P71_A0.6800.64
216_I242_D0.6780.63
168_E172_L0.6760.63
178_Y181_D0.6720.63
136_V151_N0.6700.62
257_D268_I0.6700.62
232_I240_T0.6690.62
113_E143_A0.6660.62
40_L80_L0.6630.62
39_F95_W0.6610.61
275_S279_R0.6610.61
121_I152_I0.6600.61
153_P178_Y0.6600.61
195_D199_A0.6590.61
136_V148_E0.6590.61
209_H220_P0.6580.61
56_D297_I0.6570.61
184_V281_V0.6540.61
138_S152_I0.6540.61
159_A190_Q0.6530.60
222_K262_A0.6530.60
96_S287_D0.6520.60
120_L278_A0.6500.60
192_L204_I0.6430.59
226_E294_N0.6380.59
82_A86_S0.6370.59
221_Y269_G0.6360.58
252_D272_I0.6330.58
37_S231_L0.6320.58
105_R177_Q0.6320.58
196_F200_H0.6310.58
22_L95_W0.6290.58
211_F227_R0.6290.58
106_V184_V0.6260.57
259_R298_V0.6250.57
68_F196_F0.6230.57
193_T196_F0.6230.57
220_P234_A0.6210.57
225_Y232_I0.6190.56
169_K179_D0.6150.56
46_N214_A0.6140.56
255_R283_W0.6120.56
28_D62_F0.6120.56
40_L91_F0.6100.55
118_H126_T0.6090.55
30_P44_G0.6070.55
130_M286_E0.6070.55
256_V260_D0.6060.55
151_N155_H0.6060.55
43_H74_R0.6030.54
48_I57_P0.6030.54
263_E270_R0.6020.54
35_A41_F0.6010.54
131_A182_V0.5990.54
20_G77_K0.5980.54
103_L106_V0.5970.54
65_R234_A0.5960.54
281_V284_H0.5950.53
46_N55_T0.5940.53
93_M187_R0.5940.53
191_I291_V0.5940.53
162_D166_E0.5930.53
130_M136_V0.5920.53
35_A42_E0.5900.53
252_D275_S0.5890.53
68_F138_S0.5890.53
97_L225_Y0.5880.53
23_L49_E0.5840.52
33_V62_F0.5820.52
161_K190_Q0.5820.52
25_S50_S0.5790.52
216_I239_V0.5780.51
170_K177_Q0.5780.51
117_L136_V0.5770.51
134_A184_V0.5750.51
90_K102_E0.5710.51
160_N165_A0.5710.51
161_K179_D0.5700.50
41_F295_K0.5700.50
183_I204_I0.5690.50
137_I174_L0.5690.50
32_I55_T0.5690.50
111_S187_R0.5680.50
260_D264_A0.5670.50
58_E225_Y0.5670.50
154_F240_T0.5660.50
143_A146_L0.5660.50
18_D99_L0.5650.50
145_E149_R0.5650.50
168_E192_L0.5630.50
67_E100_A0.5630.50
204_I214_A0.5620.49
124_W138_S0.5610.49
87_V91_F0.5610.49
121_I150_L0.5600.49
182_V284_H0.5600.49
192_L197_V0.5590.49
134_A152_I0.5570.49
47_I270_R0.5570.49
174_L178_Y0.5550.49
200_H203_R0.5540.48
223_R226_E0.5540.48
23_L290_I0.5540.48
236_S295_K0.5520.48
34_S122_W0.5520.48
136_V144_R0.5520.48
136_V149_R0.5510.48
176_E200_H0.5510.48
118_H150_L0.5510.48
138_S242_D0.5500.48
146_L161_K0.5500.48
156_Y279_R0.5490.48
113_E270_R0.5480.48
211_F253_I0.5460.47
140_H159_A0.5450.47
92_D203_R0.5440.47
24_V64_L0.5420.47
202_N240_T0.5410.47
55_T214_A0.5390.47
64_L239_V0.5390.47
142_E256_V0.5390.47
194_P256_V0.5390.47
78_S179_D0.5380.46
176_E179_D0.5380.46
106_V281_V0.5370.46
209_H258_H0.5370.46
161_K166_E0.5350.46
136_V176_E0.5340.46
35_A38_A0.5320.46
41_F214_A0.5290.45
88_A93_M0.5290.45
201_P241_N0.5270.45
42_E89_E0.5270.45
193_T236_S0.5270.45
64_L93_M0.5260.45
20_G191_I0.5260.45
175_L180_V0.5260.45
46_N168_E0.5250.45
48_I268_I0.5250.45
66_I236_S0.5250.45
201_P291_V0.5240.45
118_H257_D0.5230.45
141_E154_F0.5220.45
23_L214_A0.5210.44
109_F204_I0.5190.44
20_G99_L0.5190.44
150_L214_A0.5180.44
109_F175_L0.5170.44
36_V84_F0.5150.44
33_V47_I0.5130.43
50_S62_F0.5110.43
111_S140_H0.5110.43
63_F276_V0.5110.43
138_S156_Y0.5110.43
172_L183_I0.5100.43
227_R230_K0.5100.43
84_F87_V0.5100.43
211_F276_V0.5100.43
56_D292_H0.5090.43
240_T250_E0.5090.43
223_R266_K0.5080.43
155_H177_Q0.5080.43
36_V137_I0.5070.43
174_L177_Q0.5070.43
76_K87_V0.5060.43
87_V90_K0.5050.42
173_E199_A0.5040.42
125_Q199_A0.5030.42
62_F116_C0.5020.42
87_V174_L0.5020.42
79_S85_A0.5010.42
38_A82_A0.5000.42
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3obiA 4 0.9433 100 0.322 Contact Map
3louA 3 0.9333 100 0.324 Contact Map
3nrbA 4 0.94 100 0.326 Contact Map
3n0vA 4 0.9333 100 0.335 Contact Map
3w7bA 3 0.9467 100 0.339 Contact Map
3o1lA 3 0.9833 100 0.34 Contact Map
3tqrA 2 0.6533 100 0.614 Contact Map
3aufA 2 0.6967 100 0.616 Contact Map
1jkxA 2 0.6567 100 0.617 Contact Map
2ywrA 2 0.66 100 0.621 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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