GREMLIN Database
PBUO - Guanine/hypoxanthine permease PbuO
UniProt: O34978 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 432 (422)
Sequences: 3195 (1816)
Seq/√Len: 88.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
4_L9_T4.6231.00
3_H6_E4.3511.00
155_D161_T3.9471.00
62_A322_L3.9011.00
17_A315_G3.5531.00
402_P422_A3.5081.00
38_N83_F3.2831.00
410_K413_E3.1651.00
315_G320_T3.1311.00
57_G289_A3.1231.00
379_L400_S3.0661.00
264_V311_V3.0321.00
11_I319_L2.9981.00
4_L319_L2.9591.00
59_L325_A2.8751.00
15_I323_T2.8551.00
55_I329_A2.8131.00
70_A324_V2.7081.00
29_V51_I2.6831.00
69_I303_A2.6331.00
4_L11_I2.5601.00
107_V111_I2.4201.00
221_L224_G2.4191.00
176_G199_T2.3971.00
180_S195_G2.3761.00
405_K413_E2.2841.00
17_A320_T2.2501.00
7_Q317_T2.2321.00
172_L199_T2.2181.00
192_L351_A2.1451.00
184_M358_L2.1051.00
15_I19_L2.0751.00
3_H66_N2.0591.00
10_S13_Q2.0581.00
175_V202_I2.0581.00
233_A236_D2.0471.00
75_M78_N2.0441.00
149_A173_T2.0291.00
143_F386_L2.0221.00
414_I419_I1.9971.00
55_I59_L1.9681.00
59_L63_L1.9611.00
326_V330_A1.9441.00
194_I198_A1.9431.00
363_V381_I1.9381.00
58_T325_A1.9331.00
147_R159_L1.9301.00
85_V249_F1.9161.00
183_L194_I1.8991.00
411_W419_I1.8941.00
179_I198_A1.8831.00
177_L353_I1.8561.00
59_L322_L1.8461.00
406_A411_W1.8321.00
181_V375_F1.8111.00
60_W64_A1.8041.00
371_M375_F1.7851.00
29_V331_S1.7811.00
221_L225_L1.7721.00
372_D408_R1.7601.00
182_V407_A1.7561.00
56_V296_F1.7491.00
342_S345_A1.7281.00
406_A419_I1.7261.00
220_H224_G1.7251.00
15_I327_M1.7221.00
147_R388_S1.7001.00
143_F147_R1.6991.00
216_M334_F1.6971.00
110_I291_T1.6951.00
323_T326_V1.6891.00
175_V179_I1.6891.00
184_M354_I1.6841.00
154_A159_L1.6711.00
119_K123_E1.6601.00
143_F160_V1.6541.00
100_S234_F1.6221.00
32_N78_N1.6151.00
364_S369_K1.6151.00
164_N167_S1.6021.00
222_P225_L1.6011.00
56_V292_V1.6001.00
411_W414_I1.5911.00
54_S72_A1.5791.00
96_A228_S1.5671.00
128_N373_E1.5621.00
28_I32_N1.5541.00
372_D405_K1.5491.00
145_G353_I1.5451.00
55_I325_A1.5391.00
201_L205_F1.5341.00
380_V397_G1.5321.00
184_M191_A1.5161.00
67_Y276_L1.5081.00
48_T332_M1.5071.00
37_A43_F1.4911.00
403_I407_A1.4891.00
94_T97_T1.4660.99
379_L396_L1.4650.99
278_N281_K1.4470.99
38_N82_A1.4400.99
131_Y365_N1.4320.99
74_G302_T1.4250.99
266_E365_N1.4100.99
183_L188_V1.3860.99
189_S313_A1.3850.99
171_I175_V1.3720.99
366_M374_A1.3710.99
229_N295_V1.3640.99
383_A396_L1.3560.99
19_L327_M1.3560.99
152_V387_T1.3540.99
123_E275_K1.3520.99
182_V403_I1.3500.99
135_T363_V1.3420.99
41_V46_V1.3400.99
180_S354_I1.3400.99
60_W285_A1.3370.99
119_K280_R1.3340.99
261_M282_A1.3320.99
103_F294_A1.3280.99
220_H223_E1.3270.99
53_A301_T1.3220.99
25_M70_A1.3130.99
324_V331_S1.3120.99
180_S184_M1.3010.99
382_L386_L1.2920.99
253_T300_P1.2880.99
221_L226_M1.2790.99
405_K410_K1.2590.98
41_V95_Y1.2550.98
13_Q17_A1.2530.98
146_L353_I1.2490.98
176_G350_P1.2370.98
114_L284_L1.2310.98
197_A201_L1.2290.98
82_A87_S1.2250.98
67_Y278_N1.2240.98
118_R286_D1.2240.98
71_I301_T1.2100.98
32_N35_I1.2090.98
360_M381_I1.2020.98
402_P414_I1.2000.98
100_S237_V1.1970.98
253_T290_T1.1910.98
416_P420_V1.1900.98
426_F430_F1.1860.98
32_N50_T1.1770.97
4_L7_Q1.1710.97
110_I284_L1.1680.97
271_M276_L1.1650.97
415_H419_I1.1570.97
107_V291_T1.1540.97
178_L403_I1.1460.97
167_S170_V1.1460.97
221_L227_I1.1410.97
7_Q278_N1.1400.97
291_T294_A1.1390.97
319_L323_T1.1380.97
179_I195_G1.1370.97
368_W374_A1.1360.97
205_F209_L1.1340.97
32_N75_M1.1310.97
4_L10_S1.1310.97
222_P226_M1.1310.97
200_A341_L1.1280.97
234_F237_V1.1280.97
319_L322_L1.1270.97
67_Y282_A1.1260.97
62_A321_A1.1210.97
220_H225_L1.1210.97
172_L206_T1.1190.97
257_T303_A1.1150.96
288_T292_V1.1120.96
181_V404_V1.1060.96
179_I202_I1.1000.96
31_V75_M1.0960.96
116_P280_R1.0960.96
4_L66_N1.0950.96
51_I55_I1.0910.96
104_T242_L1.0890.96
400_S404_V1.0830.96
221_L228_S1.0800.96
202_I206_T1.0760.96
85_V245_V1.0740.96
110_I287_S1.0740.96
373_E405_K1.0730.96
183_L191_A1.0720.96
172_L203_A1.0650.95
132_G380_V1.0530.95
30_V339_S1.0510.95
325_A329_A1.0500.95
15_I326_V1.0490.95
265_A269_G1.0490.95
142_A378_F1.0470.95
233_A238_I1.0470.95
266_E271_M1.0430.95
323_T327_M1.0430.95
79_A83_F1.0420.95
105_A253_T1.0390.95
111_I115_T1.0370.95
156_E159_L1.0330.95
349_S353_I1.0310.95
383_A387_T1.0290.94
287_S291_T1.0210.94
54_S58_T1.0190.94
106_G290_T1.0180.94
224_G227_I1.0170.94
15_I319_L1.0140.94
185_V375_F1.0140.94
423_I427_I1.0110.94
395_A429_L1.0110.94
157_S161_T1.0110.94
12_K16_I1.0100.94
215_F337_L1.0090.94
4_L323_T1.0060.94
225_L228_S1.0020.94
200_A340_A1.0000.94
81_L99_F1.0000.94
144_I388_S0.9990.94
135_T381_I0.9950.93
372_D376_P0.9950.93
186_L409_G0.9910.93
121_L254_I0.9890.93
330_A334_F0.9840.93
24_T309_A0.9810.93
147_R154_A0.9810.93
292_V296_F0.9780.93
16_I313_A0.9760.93
185_V371_M0.9750.93
267_Q312_A0.9700.93
272_K275_K0.9700.93
142_A382_L0.9680.92
25_M304_F0.9650.92
175_V206_T0.9640.92
66_N317_T0.9620.92
223_E226_M0.9610.92
370_E405_K0.9610.92
120_Q417_L0.9600.92
292_V295_V0.9570.92
151_I353_I0.9520.92
224_G228_S0.9520.92
155_D158_N0.9500.92
212_S215_F0.9460.92
69_I270_L0.9430.91
205_F208_Q0.9390.91
180_S192_L0.9360.91
250_L254_I0.9300.91
13_Q189_S0.9270.91
220_H227_I0.9260.91
112_L250_L0.9230.91
61_M71_I0.9210.91
199_T203_A0.9190.90
48_T52_I0.9180.90
331_S335_S0.9110.90
134_T263_G0.9110.90
80_Y85_V0.9100.90
218_L222_P0.9080.90
69_I264_V0.9040.90
215_F334_F0.9030.90
39_A46_V0.9000.89
35_I82_A0.9000.89
101_A245_V0.8980.89
270_L303_A0.8970.89
289_A301_T0.8930.89
253_T302_T0.8920.89
290_T300_P0.8910.89
49_A298_T0.8880.89
97_T236_D0.8870.89
216_M221_L0.8860.89
64_A284_L0.8830.89
227_I295_V0.8820.89
26_V29_V0.8820.89
158_N161_T0.8790.88
195_G351_A0.8780.88
219_P229_N0.8770.88
320_T324_V0.8760.88
179_I194_I0.8750.88
39_A86_V0.8720.88
220_H226_M0.8700.88
379_L383_A0.8690.88
127_N131_Y0.8690.88
360_M363_V0.8690.88
233_A237_V0.8660.88
42_P45_Q0.8630.87
84_H88_A0.8610.87
104_T246_V0.8600.87
132_G381_I0.8570.87
398_F421_F0.8550.87
146_L151_I0.8550.87
53_A296_F0.8530.87
103_F295_V0.8510.87
143_F152_V0.8460.86
41_V96_A0.8450.86
50_T78_N0.8430.86
175_V198_A0.8420.86
44_D48_T0.8420.86
257_T287_S0.8410.86
154_A158_N0.8400.86
370_E410_K0.8380.86
120_Q123_E0.8370.86
182_V408_R0.8350.86
133_I251_L0.8340.86
57_G301_T0.8340.86
253_T286_D0.8330.86
356_G378_F0.8320.85
6_E66_N0.8320.85
72_A304_F0.8300.85
64_A281_K0.8290.85
409_G412_R0.8280.85
203_A209_L0.8260.85
111_I114_L0.8250.85
308_S312_A0.8240.85
30_V341_L0.8210.85
113_S284_L0.8160.84
105_A300_P0.8140.84
58_T322_L0.8130.84
172_L202_I0.8120.84
29_V47_F0.8090.84
84_H87_S0.8090.84
139_L360_M0.8080.84
30_V34_V0.8020.83
83_F87_S0.8020.83
80_Y84_H0.8020.83
139_L385_P0.8020.83
366_M378_F0.8000.83
100_S235_G0.7960.83
226_M229_N0.7940.83
34_V43_F0.7940.83
28_I78_N0.7930.83
190_G313_A0.7930.83
219_P333_F0.7880.82
7_Q270_L0.7880.82
77_L256_D0.7830.82
389_S392_T0.7820.82
153_A157_S0.7750.81
217_S220_H0.7740.81
194_I197_A0.7730.81
105_A250_L0.7730.81
174_L178_L0.7710.81
57_G292_V0.7690.81
62_A285_A0.7690.81
69_I263_G0.7670.80
253_T257_T0.7660.80
218_L224_G0.7530.79
143_F159_L0.7530.79
265_A276_L0.7510.79
85_V101_A0.7500.79
173_T353_I0.7500.79
30_V338_V0.7460.79
46_V49_A0.7440.78
372_D410_K0.7420.78
195_G199_T0.7390.78
258_T368_W0.7390.78
54_S57_G0.7360.78
11_I15_I0.7330.77
254_I428_Q0.7300.77
35_I157_S0.7290.77
361_G364_S0.7290.77
200_A345_A0.7280.77
322_L326_V0.7270.77
235_G239_H0.7270.77
179_I199_T0.7260.77
21_T70_A0.7240.76
115_T119_K0.7230.76
153_A160_V0.7220.76
404_V408_R0.7210.76
396_L400_S0.7200.76
181_V354_I0.7200.76
223_E228_S0.7190.76
28_I50_T0.7180.76
53_A57_G0.7170.76
157_S160_V0.7170.76
32_N299_S0.7160.76
402_P418_V0.7150.76
357_S374_A0.7140.76
218_L221_L0.7140.76
218_L223_E0.7130.75
403_I409_G0.7130.75
267_Q362_S0.7130.75
363_V368_W0.7080.75
18_G323_T0.7070.75
63_L66_N0.7050.75
204_F209_L0.7030.74
293_G302_T0.7020.74
360_M378_F0.7010.74
132_G377_A0.7010.74
383_A392_T0.6980.74
283_L425_F0.6970.74
54_S71_I0.6960.74
366_M377_A0.6950.74
193_F342_S0.6940.73
63_L322_L0.6940.73
132_G366_M0.6930.73
421_F424_L0.6930.73
124_A417_L0.6920.73
154_A157_S0.6920.73
35_I78_N0.6900.73
37_A83_F0.6890.73
101_A242_L0.6870.73
219_P227_I0.6860.73
217_S221_L0.6850.72
155_D160_V0.6840.72
88_A91_G0.6840.72
58_T321_A0.6830.72
203_A208_Q0.6800.72
395_A399_I0.6800.72
173_T349_S0.6740.71
186_L189_S0.6730.71
196_M342_S0.6700.71
9_T319_L0.6690.71
237_V241_G0.6680.71
119_K275_K0.6680.71
34_V83_F0.6670.70
287_S290_T0.6660.70
158_N162_L0.6620.70
380_V384_M0.6610.70
156_E161_T0.6610.70
24_T405_K0.6610.70
265_A268_A0.6610.70
51_I332_M0.6590.70
180_S351_A0.6580.69
41_V45_Q0.6580.69
212_S216_M0.6570.69
21_T310_G0.6560.69
235_G238_I0.6540.69
180_S199_T0.6520.69
57_G285_A0.6490.68
200_A342_S0.6480.68
146_L383_A0.6470.68
103_F291_T0.6460.68
80_Y83_F0.6430.68
172_L208_Q0.6430.68
327_M330_A0.6420.68
28_I304_F0.6420.68
395_A425_F0.6410.67
108_L111_I0.6380.67
171_I174_L0.6380.67
177_L354_I0.6350.67
178_L182_V0.6350.67
196_M345_A0.6350.67
150_G167_S0.6330.66
133_I421_F0.6310.66
149_A349_S0.6300.66
59_L285_A0.6290.66
36_L299_S0.6290.66
51_I54_S0.6280.66
109_F300_P0.6280.66
387_T392_T0.6280.66
129_L418_V0.6260.66
80_Y248_S0.6250.65
60_W292_V0.6250.65
155_D159_L0.6250.65
175_V199_T0.6240.65
159_L162_L0.6240.65
392_T428_Q0.6240.65
129_L398_F0.6220.65
251_L395_A0.6220.65
165_L168_P0.6200.65
70_A304_F0.6190.65
256_D260_T0.6180.65
93_I97_T0.6170.64
160_V387_T0.6170.64
78_N299_S0.6140.64
59_L329_A0.6140.64
152_V160_V0.6120.64
257_T290_T0.6100.64
261_M300_P0.6100.64
177_L379_L0.6080.63
267_Q361_G0.6070.63
2_F9_T0.6060.63
128_N377_A0.6030.63
326_V412_R0.6030.63
24_T303_A0.6010.62
224_G229_N0.6000.62
173_T177_L0.6000.62
392_T429_L0.6000.62
77_L302_T0.5990.62
37_A46_V0.5980.62
100_S229_N0.5970.62
154_A160_V0.5960.62
288_T291_T0.5950.62
121_L265_A0.5930.61
83_F86_V0.5920.61
225_L229_N0.5920.61
182_V186_L0.5910.61
19_L323_T0.5910.61
219_P224_G0.5910.61
291_T303_A0.5900.61
204_F208_Q0.5880.61
74_G253_T0.5860.61
56_V60_W0.5860.61
64_A288_T0.5860.61
34_V38_N0.5850.60
291_T295_V0.5850.60
202_I205_F0.5840.60
32_N79_A0.5830.60
71_I253_T0.5830.60
70_A351_A0.5820.60
354_I375_F0.5820.60
354_I358_L0.5820.60
42_P46_V0.5810.60
87_S93_I0.5810.60
39_A87_S0.5790.60
69_I276_L0.5780.59
53_A293_G0.5770.59
276_L279_V0.5770.59
110_I114_L0.5760.59
367_N373_E0.5750.59
268_A271_M0.5750.59
147_R150_G0.5750.59
86_V94_T0.5730.59
137_I256_D0.5730.59
108_L250_L0.5720.59
238_I242_L0.5720.59
414_I422_A0.5710.59
13_Q315_G0.5710.59
45_Q224_G0.5700.58
100_S104_T0.5690.58
109_F113_S0.5660.58
257_T391_S0.5650.58
25_M72_A0.5630.57
85_V93_I0.5630.57
423_I426_F0.5600.57
153_A159_L0.5600.57
177_L400_S0.5600.57
250_L264_V0.5570.57
4_L8_Q0.5560.57
334_F338_V0.5560.57
216_M333_F0.5550.56
179_I183_L0.5540.56
253_T303_A0.5530.56
5_K8_Q0.5520.56
122_I258_T0.5520.56
4_L15_I0.5510.56
271_M279_V0.5510.56
170_V173_T0.5510.56
238_I241_G0.5500.56
26_V309_A0.5500.56
69_I282_A0.5500.56
34_V37_A0.5490.56
210_H213_K0.5480.55
157_S343_G0.5480.55
50_T75_M0.5460.55
134_T266_E0.5460.55
218_L225_L0.5450.55
109_F287_S0.5450.55
258_T290_T0.5440.55
173_T344_I0.5440.55
157_S382_L0.5430.55
122_I207_G0.5420.55
379_L404_V0.5420.55
218_L226_M0.5410.55
61_M286_D0.5410.55
91_G245_V0.5410.55
175_V205_F0.5400.54
365_N369_K0.5400.54
211_F214_G0.5390.54
109_F252_V0.5380.54
216_M220_H0.5370.54
312_A358_L0.5350.54
381_I384_M0.5350.54
58_T63_L0.5350.54
234_F238_I0.5340.54
81_L249_F0.5340.54
5_K107_V0.5330.53
131_Y135_T0.5330.53
77_L252_V0.5330.53
131_Y366_M0.5320.53
136_G384_M0.5320.53
204_F340_A0.5310.53
236_D240_H0.5300.53
241_G244_A0.5290.53
3_H7_Q0.5280.53
190_G194_I0.5280.53
153_A161_T0.5270.53
222_P227_I0.5270.53
98_A102_V0.5270.53
177_L371_M0.5270.53
372_D404_V0.5260.53
99_F296_F0.5260.53
102_V249_F0.5260.53
86_V98_A0.5250.52
59_L62_A0.5250.52
20_T309_A0.5240.52
58_T70_A0.5240.52
284_L291_T0.5240.52
97_T249_F0.5230.52
43_F47_F0.5210.52
133_I262_I0.5200.52
198_A205_F0.5200.52
7_Q10_S0.5200.52
2_F7_Q0.5190.52
165_L174_L0.5180.51
154_A162_L0.5180.51
330_A333_F0.5180.51
406_A414_I0.5170.51
21_T25_M0.5170.51
166_H170_V0.5170.51
73_P298_T0.5140.51
373_E376_P0.5140.51
217_S339_S0.5140.51
47_F335_S0.5120.51
184_M371_M0.5110.50
181_V185_V0.5100.50
13_Q16_I0.5080.50
181_V357_S0.5070.50
112_L193_F0.5070.50
207_G210_H0.5070.50
131_Y266_E0.5070.50
54_S301_T0.5070.50
70_A277_P0.5070.50
42_P95_Y0.5060.50
265_A271_M0.5060.50
100_S236_D0.5050.50
28_I299_S0.5050.50
154_A161_T0.5050.50
326_V329_A0.5040.50
142_A388_S0.5040.50
145_G182_V0.5040.50
116_P415_H0.5030.49
146_L152_V0.5030.49
163_G170_V0.5030.49
188_V327_M0.5030.49
32_N298_T0.5010.49
130_K350_P0.5010.49
14_E22_F0.5010.49
402_P411_W0.5010.49
51_I428_Q0.5000.49
26_V394_I0.5000.49
9_T66_N0.5000.49
24_T373_E0.5000.49
260_T264_V0.5000.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3qe7A 2 0.8588 100 0.652 Contact Map
3lt7A 3 0.0671 3.9 0.985 Contact Map
4f35D 2 0.5394 2.7 0.986 Contact Map
3pzfA 1 0.1157 2.5 0.986 Contact Map
3o7qA 1 0.4051 2.2 0.986 Contact Map
4ujpg 1 0.0764 2.1 0.987 Contact Map
3j45G 1 0.1366 2.1 0.987 Contact Map
3j01B 1 0.2662 1.9 0.987 Contact Map
4j2kA 2 0.1134 1.9 0.987 Contact Map
3s8kA 2 0.1389 1.8 0.987 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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