GREMLIN Database
ZNUA - High-affinity zinc uptake system binding-protein ZnuA
UniProt: O34966 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 319 (288)
Sequences: 5829 (4656)
Seq/√Len: 274.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
157_Q190_Q4.1351.00
171_D178_Y3.9701.00
57_K174_N3.8661.00
54_V58_G3.8541.00
57_K177_Y3.7181.00
164_T182_S3.4941.00
195_L305_K3.4861.00
152_S221_E3.3341.00
193_D222_Y3.1951.00
161_K186_I3.1731.00
175_K179_E2.9301.00
171_D174_N2.6371.00
157_Q186_I2.6181.00
174_N178_Y2.6151.00
50_T90_V2.5901.00
168_V179_E2.5791.00
164_T168_V2.5011.00
52_Q188_K2.4801.00
38_H59_D2.4601.00
160_V182_S2.3901.00
266_S270_D2.3011.00
47_Y62_L2.1431.00
49_F156_A2.1021.00
174_N177_Y2.0781.00
199_T312_K2.0231.00
164_T179_E2.0111.00
50_T167_I2.0031.00
207_E225_K2.0011.00
168_V175_K1.9751.00
40_V86_A1.9701.00
61_D82_N1.9411.00
193_D197_R1.9371.00
177_Y181_N1.9271.00
270_D274_S1.9271.00
50_T163_I1.9261.00
182_S186_I1.8981.00
140_E143_H1.8301.00
47_Y51_K1.8251.00
250_A276_I1.8201.00
196_Y308_L1.8201.00
36_K59_D1.7681.00
248_T252_E1.7621.00
298_L302_D1.7201.00
249_Y253_H1.7201.00
184_E188_K1.6471.00
160_V185_Y1.6391.00
154_V221_E1.6331.00
195_L309_D1.6221.00
120_G162_N1.6091.00
90_V117_A1.6001.00
166_Q169_K1.5841.00
209_I250_A1.5631.00
49_F185_Y1.5621.00
176_E180_K1.5601.00
299_G302_D1.5461.00
188_K301_I1.5421.00
244_A248_T1.5121.00
88_L166_Q1.5091.00
166_Q170_Q1.5011.00
79_D82_N1.4731.00
195_L199_T1.4681.00
155_L158_K1.4661.00
180_K184_E1.4651.00
116_N119_K1.4571.00
54_V167_I1.4521.00
57_K171_D1.4451.00
88_L170_Q1.4351.00
121_I124_M1.4331.00
309_D313_D1.4191.00
38_H87_D1.4041.00
207_E253_H1.4031.00
199_T309_D1.4031.00
38_H61_D1.4001.00
154_V193_D1.3951.00
179_E183_K1.3931.00
53_I182_S1.3891.00
117_A159_E1.3801.00
48_E52_Q1.3751.00
259_Y314_S1.3411.00
158_K162_N1.3311.00
183_K187_A1.3281.00
194_K198_T1.3171.00
196_Y218_L1.3111.00
37_L170_Q1.3101.00
39_V167_I1.3071.00
188_K305_K1.3051.00
190_Q194_K1.2981.00
113_V169_K1.2971.00
218_L308_L1.2871.00
57_K181_N1.2851.00
186_I190_Q1.2721.00
149_V159_E1.2691.00
161_K164_T1.2671.00
52_Q185_Y1.2651.00
183_K186_I1.2571.00
41_T47_Y1.2471.00
78_K81_A1.2421.00
197_R201_K1.2391.00
191_D195_L1.2381.00
242_S245_K1.2361.00
49_F160_V1.2361.00
196_Y304_M1.2201.00
91_Y116_N1.2201.00
53_I160_V1.2181.00
90_V115_V1.2131.00
118_S121_I1.2071.00
81_A85_D1.2041.00
246_L272_L1.1971.00
152_S155_L1.1931.00
306_Q310_A1.1901.00
219_A224_L1.1881.00
187_A191_D1.1771.00
124_M220_K1.1751.00
165_A168_V1.1681.00
73_W79_D1.1581.00
39_V58_G1.1551.00
91_Y100_V1.1421.00
113_V170_Q1.1391.00
305_K309_D1.1361.00
162_N166_Q1.1271.00
52_Q301_I1.1211.00
75_P99_W1.1191.00
250_A278_A1.1171.00
247_K275_E1.1121.00
176_E179_E1.1121.00
187_A190_Q1.1111.00
181_N184_E1.1011.00
161_K165_A1.0861.00
177_Y180_K1.0761.00
238_P268_V1.0711.00
71_H148_H1.0611.00
247_K272_L1.0571.00
46_M117_A1.0521.00
205_K256_K1.0501.00
291_K295_D1.0431.00
55_K177_Y1.0421.00
188_K191_D1.0391.00
146_D216_G1.0341.00
48_E185_Y1.0311.00
180_K183_K1.0261.00
17_L20_A1.0171.00
53_I178_Y1.0161.00
41_T62_L1.0141.00
39_V88_L1.0131.00
90_V163_I1.0131.00
37_L54_V1.0131.00
14_C17_L1.0071.00
309_D312_K1.0021.00
49_F163_I0.9961.00
184_E187_A0.9901.00
161_K182_S0.9901.00
154_V157_Q0.9881.00
257_V281_E0.9871.00
158_K161_K0.9781.00
206_K223_G0.9771.00
227_V249_Y0.9741.00
213_T228_P0.9731.00
92_N159_E0.9661.00
118_S123_L0.9601.00
215_F283_L0.9531.00
141_H144_A0.9521.00
155_L220_K0.9491.00
121_I155_L0.9461.00
37_L58_G0.9431.00
139_H142_S0.9431.00
260_F280_T0.9411.00
13_A16_L0.9411.00
306_Q309_D0.9401.00
157_Q161_K0.9371.00
40_V82_N0.9361.00
41_T50_T0.9251.00
298_L306_Q0.9251.00
115_V166_Q0.9241.00
153_P189_L0.9221.00
153_P221_E0.9201.00
197_R221_E0.9171.00
155_L159_E0.9111.00
200_A308_L0.9021.00
247_K251_K0.8961.00
40_V61_D0.8961.00
57_K178_Y0.8951.00
40_V63_L0.8931.00
273_A280_T0.8931.00
193_D221_E0.8911.00
258_I273_A0.8871.00
54_V60_V0.8731.00
156_A304_M0.8731.00
207_E227_V0.8681.00
164_T186_I0.8671.00
14_C18_V0.8651.00
185_Y189_L0.8621.00
39_V54_V0.8541.00
41_T46_M0.8501.00
208_F219_A0.8471.00
163_I167_I0.8441.00
46_M159_E0.8371.00
69_E72_D0.8341.00
88_L115_V0.8260.99
273_A277_G0.8230.99
209_I246_L0.8190.99
91_Y114_F0.8160.99
208_F226_Q0.8150.99
165_A169_K0.8110.99
245_K248_T0.8090.99
160_V186_I0.8080.99
37_L87_D0.8060.99
89_F100_V0.8050.99
308_L312_K0.8040.99
102_S105_K0.8030.99
41_T60_V0.8010.99
94_E123_L0.7930.99
162_N165_A0.7920.99
138_E141_H0.7880.99
113_V166_Q0.7880.99
90_V166_Q0.7880.99
38_H85_D0.7870.99
89_F112_A0.7870.99
300_Y304_M0.7830.99
208_F224_L0.7710.99
210_T215_F0.7690.99
190_Q193_D0.7680.99
198_T201_K0.7670.99
257_V314_S0.7660.99
153_P304_M0.7660.99
165_A179_E0.7640.99
80_I103_A0.7630.99
15_F18_V0.7600.99
53_I181_N0.7510.99
49_F159_E0.7500.99
52_Q181_N0.7440.99
203_A206_K0.7440.99
48_E51_K0.7430.99
89_F104_E0.7410.99
195_L312_K0.7390.99
195_L308_L0.7360.99
179_E182_S0.7360.99
161_K183_K0.7270.99
121_I126_G0.7220.99
225_K253_H0.7170.99
245_K249_Y0.7170.99
189_L192_L0.7110.98
219_A226_Q0.7090.98
248_T251_K0.7080.98
208_F311_L0.7070.98
241_A244_A0.7050.98
304_M308_L0.7040.98
117_A162_N0.7040.98
281_E310_A0.7000.98
154_V197_R0.6980.98
195_L198_T0.6960.98
267_K270_D0.6950.98
239_S242_S0.6940.98
191_D194_K0.6940.98
21_C24_S0.6940.98
168_V182_S0.6920.98
39_V50_T0.6910.98
50_T54_V0.6890.98
200_A224_L0.6880.98
149_V155_L0.6880.98
40_V83_I0.6870.98
69_E234_P0.6850.98
39_V60_V0.6850.98
46_M92_N0.6790.98
104_E114_F0.6770.98
63_L83_I0.6740.98
87_D170_Q0.6730.98
54_V57_K0.6730.98
78_K82_N0.6730.98
100_V104_E0.6710.98
211_Q260_F0.6710.98
302_D305_K0.6680.98
52_Q299_G0.6670.98
203_A223_G0.6660.98
9_F12_A0.6650.98
172_P175_K0.6640.98
263_I284_N0.6640.98
65_P68_V0.6630.98
47_Y60_V0.6620.98
156_A159_E0.6600.98
12_A15_F0.6600.98
168_V178_Y0.6570.97
202_K206_K0.6540.97
37_L171_D0.6530.97
55_K184_E0.6480.97
189_L300_Y0.6480.97
53_I164_T0.6460.97
160_V164_T0.6450.97
210_T213_T0.6420.97
58_G167_I0.6420.97
259_Y283_L0.6380.97
115_V162_N0.6370.97
295_D298_L0.6360.97
46_M90_V0.6300.97
157_Q164_T0.6300.97
188_K299_G0.6290.97
94_E118_S0.6270.97
231_G234_P0.6260.97
263_I288_G0.6260.97
269_A273_A0.6260.97
196_Y200_A0.6230.96
164_T178_Y0.6220.96
83_I103_A0.6220.96
275_E278_A0.6180.96
279_K313_D0.6180.96
42_T97_E0.6170.96
153_P193_D0.6160.96
37_L167_I0.6120.96
197_R223_G0.6090.96
260_F269_A0.6090.96
148_H212_H0.6080.96
53_I168_V0.6080.96
121_I158_K0.6070.96
209_I255_V0.6040.96
260_F264_A0.6020.96
94_E147_P0.6010.96
280_T283_L0.6010.96
212_H261_E0.6000.96
156_A300_Y0.5990.96
160_V189_L0.5990.96
213_T233_S0.5980.96
290_S295_D0.5970.96
218_L311_L0.5930.95
258_I269_A0.5920.95
247_K276_I0.5920.95
92_N96_M0.5910.95
285_T290_S0.5900.95
209_I249_Y0.5900.95
98_T101_P0.5880.95
194_K197_R0.5840.95
70_P263_I0.5810.95
271_T274_S0.5780.95
42_T91_Y0.5780.95
304_M307_N0.5770.95
286_L303_I0.5770.95
9_F16_L0.5760.95
284_N311_L0.5760.95
10_V16_L0.5730.94
44_Y48_E0.5720.94
271_T275_E0.5720.94
287_E290_S0.5710.94
51_K60_V0.5710.94
301_I305_K0.5710.94
9_F13_A0.5700.94
92_N149_V0.5690.94
153_P301_I0.5670.94
272_L276_I0.5670.94
192_L301_I0.5660.94
211_Q264_A0.5650.94
113_V117_A0.5640.94
251_K277_G0.5630.94
224_L315_L0.5630.94
227_V246_L0.5620.94
52_Q160_V0.5590.94
202_K205_K0.5590.94
77_P240_A0.5550.93
199_T203_A0.5550.93
216_G220_K0.5550.93
127_S141_H0.5540.93
83_I107_M0.5520.93
99_W103_A0.5510.93
157_Q189_L0.5500.93
12_A18_V0.5490.93
210_T226_Q0.5470.93
250_A258_I0.5470.93
17_L21_C0.5460.93
20_A23_N0.5450.93
43_F96_M0.5440.93
285_T289_L0.5430.93
18_V21_C0.5430.93
101_P105_K0.5430.93
39_V90_V0.5420.93
125_E145_M0.5410.93
263_I286_L0.5400.92
76_T79_D0.5400.92
43_F69_E0.5400.92
310_A313_D0.5400.92
262_E284_N0.5390.92
64_I70_P0.5390.92
284_N303_I0.5380.92
24_S32_S0.5370.92
45_P48_E0.5370.92
210_T219_A0.5370.92
118_S149_V0.5370.92
12_A16_L0.5360.92
19_A22_G0.5360.92
261_E264_A0.5350.92
284_N287_E0.5350.92
159_E163_I0.5340.92
172_P178_Y0.5330.92
152_S220_K0.5310.92
229_I243_L0.5300.92
10_V25_S0.5290.92
243_L271_T0.5290.92
243_L272_L0.5290.92
56_D177_Y0.5280.92
146_D212_H0.5270.92
226_Q229_I0.5270.92
185_Y301_I0.5270.92
108_G112_A0.5260.92
15_F19_A0.5260.92
260_F282_V0.5250.91
199_T308_L0.5250.91
71_H212_H0.5230.91
45_P286_L0.5230.91
55_K60_V0.5210.91
151_L304_M0.5180.91
214_A261_E0.5180.91
120_G124_M0.5170.91
191_D305_K0.5170.91
109_Q112_A0.5170.91
31_D34_G0.5160.91
218_L224_L0.5160.91
286_L289_L0.5150.91
258_I276_I0.5140.91
241_A245_K0.5140.91
182_S185_Y0.5130.90
153_P222_Y0.5120.90
156_A163_I0.5090.90
240_A244_A0.5090.90
290_S296_K0.5090.90
96_M147_P0.5080.90
211_Q261_E0.5080.90
115_V163_I0.5070.90
146_D219_A0.5070.90
200_A203_A0.5070.90
75_P103_A0.5070.90
192_L305_K0.5060.90
231_G242_S0.5050.90
48_E299_G0.5040.90
44_Y47_Y0.5030.90
192_L304_M0.5020.90
21_C25_S0.5000.89
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4xrvA 1 0.815 100 0.113 Contact Map
2o1eA 1 0.8339 100 0.12 Contact Map
4utoA 1 0.8401 100 0.138 Contact Map
3cx3A 1 0.8307 100 0.151 Contact Map
2prsA 1 0.8245 100 0.167 Contact Map
4nnoA 1 0.8464 100 0.179 Contact Map
3ujpA 4 0.8433 100 0.18 Contact Map
1pq4A 1 0.815 100 0.18 Contact Map
1toaA 1 0.8464 100 0.189 Contact Map
4h0fA 1 0.8276 100 0.19 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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