GREMLIN Database
HIS2 - Histidine biosynthesis bifunctional protein HisIE
UniProt: O34912 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 209 (195)
Sequences: 1587 (910)
Seq/√Len: 65.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
41_T70_V4.4631.00
137_T180_Y3.4751.00
18_I30_L3.2951.00
7_L15_I3.0661.00
126_E130_E2.8971.00
56_A62_E2.5911.00
146_D165_N2.4341.00
173_W199_V2.3691.00
7_L75_Y2.3161.00
48_W59_H2.2501.00
50_Y55_Q2.1871.00
47_T84_V2.1761.00
46_E59_H2.1671.00
69_A89_S2.1201.00
71_K87_E2.1161.00
67_T89_S2.1081.00
172_K199_V2.0871.00
40_K43_E2.0291.00
146_D164_K2.0151.00
88_P91_P2.0121.00
8_R11_E1.9941.00
39_E43_E1.9911.00
69_A87_E1.9871.00
35_K39_E1.9871.00
17_A33_M1.9811.00
30_L81_A1.9461.00
199_V202_K1.9081.00
121_E187_R1.8741.00
26_E98_Y1.8541.00
157_S160_I1.8541.00
31_A92_A1.8341.00
97_S102_T1.8281.00
17_A84_V1.8191.00
46_E69_A1.8181.00
153_G157_S1.8061.00
125_A187_R1.8041.00
175_A186_L1.7991.00
19_V49_F1.7781.00
150_K157_S1.7240.99
132_P138_T1.7080.99
33_M84_V1.6770.99
175_A182_L1.6580.99
73_I84_V1.6490.99
159_V175_A1.6370.99
131_M138_T1.6240.99
145_V188_E1.6230.99
95_T102_T1.5850.99
72_G85_L1.5840.99
128_Q187_R1.5610.99
46_E67_T1.5580.99
179_L182_L1.4430.98
17_A37_S1.4410.98
47_T70_V1.4390.98
171_L191_L1.4340.98
40_K48_W1.4130.98
25_K85_L1.4120.98
40_K57_L1.4000.98
56_A63_T1.3790.97
159_V178_L1.3730.97
176_A196_V1.3390.97
168_H191_L1.3380.97
49_F88_P1.3260.97
59_H62_E1.3230.97
144_G148_I1.3150.97
175_A179_L1.2840.96
168_H195_D1.2590.96
138_T142_R1.2470.95
131_M134_G1.1990.94
29_T81_A1.1770.94
22_A71_K1.1680.94
40_K44_T1.1640.93
159_V182_L1.1590.93
66_N88_P1.1530.93
128_Q141_F1.1510.93
40_K59_H1.1460.93
117_M193_L1.1430.93
23_A87_E1.1370.93
176_A199_V1.1350.93
163_A185_L1.1350.93
162_A170_E1.1130.92
29_T83_L1.1040.91
16_P30_L1.1020.91
137_T184_V1.0990.91
139_Y147_K1.0810.91
147_K161_I1.0780.90
121_E193_L1.0760.90
143_E147_K1.0730.90
49_F68_Q1.0650.90
34_N37_S1.0590.90
70_V101_F1.0530.89
166_R189_Q1.0510.89
156_A160_I1.0500.89
68_Q92_A1.0450.89
180_Y184_V1.0370.89
119_E204_H1.0250.88
192_P195_D1.0220.88
145_V189_Q1.0190.88
124_I141_F1.0150.88
168_H189_Q1.0090.87
9_F38_Y1.0080.87
116_I204_H1.0020.87
198_D201_K0.9850.86
153_G160_I0.9670.85
125_A129_A0.9580.84
38_Y42_L0.9570.84
8_R53_S0.9540.84
152_V156_A0.9530.84
27_V83_L0.9510.84
49_F84_V0.9420.83
196_V199_V0.9400.83
156_A161_I0.9360.83
134_G141_F0.9300.83
19_V86_V0.9280.83
74_R85_L0.9270.82
47_T68_Q0.9240.82
162_A171_L0.9190.82
44_T48_W0.9160.82
46_E62_E0.9130.81
68_Q86_V0.9130.81
7_L38_Y0.9120.81
19_V47_T0.9120.81
47_T86_V0.9050.81
45_K69_A0.9000.81
19_V84_V0.8960.80
68_Q88_P0.8900.80
66_N91_P0.8800.79
130_E138_T0.8790.79
66_N89_S0.8770.79
198_D202_K0.8560.77
72_G104_E0.8520.77
128_Q190_S0.8470.76
15_I82_L0.8450.76
9_F15_I0.8430.76
50_Y62_E0.8400.76
191_L195_D0.8340.75
34_N50_Y0.8250.74
183_L193_L0.8220.74
134_G142_R0.8130.73
145_V163_A0.8110.73
140_L184_V0.8100.73
163_A171_L0.8040.73
117_M183_L0.7950.72
167_D170_E0.7950.72
31_A47_T0.7920.71
113_R194_D0.7880.71
50_Y56_A0.7840.71
34_N57_L0.7820.70
70_V84_V0.7710.69
42_L73_I0.7630.69
131_M142_R0.7630.69
47_T59_H0.7570.68
124_I187_R0.7540.68
150_K153_G0.7470.67
59_H67_T0.7460.67
172_K191_L0.7450.67
165_N170_E0.7310.65
171_L189_Q0.7240.64
190_S197_L0.7230.64
124_I183_L0.7220.64
153_G161_I0.7170.64
149_L157_S0.7100.63
141_F148_I0.7080.63
118_N122_R0.7030.62
199_V203_R0.7010.62
88_P98_Y0.6990.62
10_N32_Y0.6970.62
166_R170_E0.6960.61
123_V204_H0.6960.61
194_D201_K0.6940.61
118_N121_E0.6870.60
139_Y142_R0.6860.60
21_D98_Y0.6840.60
168_H172_K0.6840.60
145_V156_A0.6830.60
121_E192_P0.6820.60
159_V196_V0.6800.60
128_Q131_M0.6780.59
194_D198_D0.6770.59
130_E134_G0.6740.59
59_H68_Q0.6730.59
147_K157_S0.6730.59
148_I188_E0.6720.59
70_V73_I0.6680.58
134_G138_T0.6660.58
54_R63_T0.6640.58
47_T92_A0.6610.57
139_Y143_E0.6580.57
91_P98_Y0.6540.57
47_T73_I0.6530.57
31_A68_Q0.6510.56
16_P32_Y0.6470.56
195_D198_D0.6440.56
194_D197_L0.6430.55
16_P81_A0.6420.55
193_L197_L0.6390.55
57_L90_G0.6380.55
152_V178_L0.6360.55
161_I165_N0.6350.54
146_D170_E0.6310.54
39_E169_E0.6300.54
145_V164_K0.6270.54
114_F197_L0.6270.54
178_L193_L0.6260.53
67_T71_K0.6250.53
22_A87_E0.6230.53
172_K195_D0.6230.53
139_Y150_K0.6220.53
164_K170_E0.6200.53
187_R190_S0.6180.52
12_D55_Q0.6170.52
10_N13_G0.6150.52
44_T59_H0.6110.52
146_D161_I0.6080.51
145_V171_L0.6060.51
18_I83_L0.6050.51
37_S47_T0.6040.51
32_Y81_A0.6010.51
159_V176_A0.5980.50
7_L82_L0.5980.50
121_E125_A0.5950.50
128_Q138_T0.5940.50
145_V165_N0.5910.49
135_A138_T0.5890.49
42_L45_K0.5890.49
12_D104_E0.5860.49
123_V141_F0.5830.48
113_R201_K0.5820.48
17_A49_F0.5790.48
21_D24_S0.5790.48
18_I27_V0.5770.48
126_E131_M0.5750.47
60_K94_H0.5720.47
37_S41_T0.5700.47
170_E173_W0.5670.47
31_A54_R0.5660.46
21_D87_E0.5650.46
48_W57_L0.5630.46
24_S98_Y0.5620.46
10_N14_L0.5600.46
53_S81_A0.5580.45
8_R54_R0.5580.45
49_F86_V0.5560.45
53_S92_A0.5550.45
63_T137_T0.5550.45
95_T103_K0.5540.45
33_M47_T0.5530.45
147_K153_G0.5530.45
124_I132_P0.5520.45
147_K170_E0.5500.45
141_F187_R0.5470.44
159_V162_A0.5450.44
149_L153_G0.5450.44
168_H190_S0.5430.44
179_L193_L0.5400.43
99_S162_A0.5400.43
150_K159_V0.5380.43
41_T47_T0.5370.43
45_K169_E0.5350.43
35_K79_Q0.5320.42
31_A59_H0.5320.42
33_M37_S0.5310.42
17_A25_K0.5310.42
29_T88_P0.5300.42
62_E122_R0.5290.42
144_G188_E0.5280.42
10_N35_K0.5240.42
171_L196_V0.5230.41
145_V149_L0.5210.41
26_E105_Q0.5210.41
29_T58_W0.5200.41
166_R188_E0.5200.41
149_L164_K0.5170.41
114_F180_Y0.5170.41
27_V30_L0.5170.41
117_M197_L0.5170.41
180_Y185_L0.5160.41
172_K179_L0.5150.41
172_K176_A0.5150.41
149_L160_I0.5140.40
138_T141_F0.5130.40
124_I178_L0.5110.40
139_Y180_Y0.5110.40
146_D150_K0.5100.40
66_N130_E0.5100.40
139_Y170_E0.5090.40
146_D160_I0.5060.40
33_M41_T0.5050.39
132_P141_F0.5050.39
152_V185_L0.5040.39
173_W202_K0.5040.39
71_K89_S0.5020.39
160_I165_N0.5000.39
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1zpsA 2 0.5837 100 0.429 Contact Map
2a7wA 4 0.4354 100 0.64 Contact Map
1yvwA 4 0.4402 100 0.647 Contact Map
1y6xA 3 0.4163 100 0.655 Contact Map
1yxbA 4 0.3971 100 0.659 Contact Map
2yxhA 2 0.4211 99.1 0.86 Contact Map
2gtaA 4 0.4211 98.6 0.882 Contact Map
1vmgA 4 0.3971 97.9 0.902 Contact Map
2oieA 3 0.4593 97.7 0.908 Contact Map
2q5zA 4 0.4115 97.5 0.911 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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