GREMLIN Database
ISCS2 - Putative cysteine desulfurase IscS 2
UniProt: O34874 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 381 (316)
Sequences: 34626 (24099)
Seq/√Len: 1355.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
270_L363_E3.4631.00
274_R366_M3.4121.00
90_H117_T3.3701.00
162_G173_F3.3351.00
270_L359_S3.2441.00
157_P160_A3.0381.00
147_H180_G2.8651.00
90_H137_R2.7161.00
187_A190_K2.6651.00
51_Q251_N2.6481.00
314_Q371_P2.6051.00
133_K167_E2.6021.00
133_K164_V2.5981.00
90_H115_D2.5721.00
95_S120_S2.5221.00
177_Y196_C2.5121.00
248_K251_N2.4931.00
131_D134_A2.4191.00
165_L171_I2.2721.00
239_H242_G2.2371.00
274_R363_E2.2061.00
314_Q368_A2.1881.00
278_I370_R2.1881.00
198_I211_A2.1771.00
270_L274_R2.1631.00
90_H140_T2.1571.00
143_V165_L2.1201.00
131_D135_A2.0441.00
364_P368_A1.9781.00
274_R277_E1.9301.00
351_I365_F1.9211.00
163_E167_E1.8911.00
162_G166_K1.8891.00
128_S131_D1.8781.00
282_V298_S1.8581.00
73_N100_S1.8571.00
57_G187_A1.8511.00
266_A359_S1.8421.00
159_E163_E1.8111.00
90_H139_D1.8101.00
310_M372_G1.8041.00
159_E190_K1.8001.00
117_T135_A1.7851.00
168_H171_I1.7831.00
67_S71_E1.7691.00
165_L173_F1.7641.00
263_T267_V1.7501.00
272_M295_I1.7441.00
303_K306_V1.7121.00
120_S131_D1.6981.00
89_K141_V1.6961.00
129_I164_V1.6381.00
119_L135_A1.6321.00
269_E273_N1.6221.00
284_N298_S1.6101.00
89_K139_D1.6011.00
282_V300_P1.5941.00
145_M161_A1.5861.00
54_R254_S1.5671.00
164_V168_H1.5661.00
309_H313_E1.5601.00
160_A163_E1.5561.00
274_R370_R1.5531.00
166_K194_D1.5481.00
122_N126_F1.5351.00
66_T72_A1.5171.00
51_Q247_A1.4911.00
175_V193_I1.4841.00
193_I196_C1.4781.00
271_F353_L1.4771.00
133_K168_H1.4731.00
142_L174_H1.4571.00
136_I164_V1.4501.00
262_D266_A1.4411.00
50_N54_R1.4311.00
76_A195_L1.4101.00
185_P188_I1.4021.00
47_A51_Q1.3851.00
258_D262_D1.3771.00
166_K192_G1.3711.00
359_S363_E1.3711.00
162_G192_G1.3621.00
145_M173_F1.3591.00
186_L190_K1.3551.00
296_N348_S1.3521.00
224_I227_G1.3491.00
129_I160_A1.3441.00
265_T269_E1.3371.00
69_A178_V1.3311.00
200_G246_L1.3281.00
62_D216_E1.3251.00
358_T361_V1.3241.00
310_M313_E1.3201.00
206_L245_S1.3121.00
148_V291_A1.2981.00
80_A172_L1.2981.00
130_E133_K1.2971.00
261_L265_T1.2931.00
185_P189_E1.2871.00
277_E370_R1.2851.00
95_S118_Y1.2811.00
263_T266_A1.2801.00
298_S347_S1.2771.00
73_N174_H1.2721.00
226_G229_Q1.2681.00
310_M314_Q1.2621.00
137_R140_T1.2601.00
122_N128_S1.2561.00
268_K272_M1.2511.00
224_I229_Q1.2511.00
158_I175_V1.2511.00
51_Q254_S1.2411.00
225_T228_S1.2401.00
130_E134_A1.2381.00
92_I135_A1.2351.00
93_A105_L1.2341.00
368_A371_P1.2331.00
256_D259_T1.2241.00
270_L362_A1.2181.00
92_I140_T1.2101.00
44_L244_V1.2041.00
223_L227_G1.1931.00
72_A197_S1.1921.00
133_K137_R1.1751.00
272_M276_S1.1721.00
132_L161_A1.1531.00
203_F246_L1.1511.00
51_Q54_R1.1491.00
164_V167_E1.1451.00
221_I225_T1.1411.00
223_L226_G1.1411.00
50_N53_K1.1361.00
52_I211_A1.1361.00
247_A251_N1.1341.00
48_A247_A1.1261.00
271_F362_A1.1201.00
59_K216_E1.1131.00
174_H197_S1.1121.00
107_Q225_T1.1051.00
195_L214_V1.1051.00
364_P367_N1.1021.00
256_D260_R1.0991.00
115_D137_R1.0981.00
311_L318_V1.0961.00
143_V171_I1.0931.00
78_K225_T1.0891.00
306_V310_M1.0881.00
273_N276_S1.0831.00
133_K160_A1.0781.00
225_T229_Q1.0761.00
186_L189_E1.0741.00
276_S287_Q1.0711.00
366_M370_R1.0621.00
367_N371_P1.0611.00
93_A118_Y1.0561.00
43_Q47_A1.0551.00
360_D363_E1.0521.00
77_L105_L1.0481.00
86_K89_K1.0481.00
163_E192_G1.0461.00
47_A247_A1.0441.00
102_T118_Y1.0401.00
85_I88_G1.0321.00
85_I114_F1.0281.00
124_D286_P1.0231.00
103_E106_E1.0221.00
129_I133_K1.0171.00
274_R367_N1.0131.00
291_A294_I1.0051.00
363_E367_N1.0041.00
318_V349_I1.0031.00
267_V362_A1.0031.00
204_H354_N1.0021.00
147_H196_C0.9881.00
59_K189_E0.9881.00
132_L143_V0.9861.00
55_S181_I0.9831.00
148_V180_G0.9831.00
137_R168_H0.9811.00
316_I364_P0.9811.00
145_M158_I0.9801.00
307_L372_G0.9761.00
181_I198_I0.9741.00
316_I368_A0.9721.00
185_P190_K0.9651.00
273_N277_E0.9641.00
280_G300_P0.9641.00
259_T262_D0.9591.00
251_N255_A0.9581.00
91_I105_L0.9461.00
166_K173_F0.9431.00
259_T263_T0.9351.00
144_S174_H0.9321.00
57_G189_E0.9321.00
212_L215_K0.9301.00
88_G141_V0.9291.00
251_N254_S0.9291.00
222_P225_T0.9201.00
291_A295_I0.9161.00
147_H158_I0.9131.00
275_L369_L0.9101.00
308_L320_T0.9101.00
307_L369_L0.9091.00
63_I213_I0.9041.00
155_V184_V0.9031.00
188_I213_I0.9021.00
61_Y216_E0.9011.00
293_H296_N0.8961.00
248_K252_L0.8951.00
224_I228_S0.8911.00
136_I140_T0.8861.00
308_L318_V0.8831.00
78_K107_Q0.8821.00
220_L224_I0.8811.00
136_I168_H0.8801.00
316_I365_F0.8801.00
96_I156_Q0.8791.00
297_F351_I0.8791.00
85_I89_K0.8761.00
117_T137_R0.8741.00
45_L243_A0.8641.00
180_G196_C0.8611.00
305_E309_H0.8561.00
309_H312_E0.8481.00
159_E191_A0.8431.00
274_R278_I0.8421.00
130_E160_A0.8371.00
119_L131_D0.8371.00
271_F365_F0.8351.00
101_V144_S0.8351.00
223_L229_Q0.8281.00
146_M150_N0.8261.00
165_L170_N0.8251.00
74_N78_K0.8211.00
174_H195_L0.8181.00
272_M283_I0.8161.00
53_K58_L0.8141.00
267_V359_S0.8081.00
147_H191_A0.8071.00
268_K295_I0.8061.00
260_R355_Y0.8001.00
162_G191_A0.7991.00
211_A215_K0.7991.00
172_L194_D0.7981.00
263_T359_S0.7971.00
290_S294_I0.7961.00
80_A84_K0.7941.00
244_V247_A0.7931.00
306_V309_H0.7891.00
92_I117_T0.7761.00
117_T140_T0.7751.00
360_D364_P0.7751.00
221_I228_S0.7701.00
353_L362_A0.7691.00
266_A270_L0.7681.00
260_R264_M0.7681.00
308_L312_E0.7661.00
136_I171_I0.7641.00
318_V351_I0.7631.00
172_L195_L0.7601.00
36_R40_E0.7581.00
47_A251_N0.7571.00
129_I132_L0.7551.00
92_I119_L0.7531.00
292_P295_I0.7511.00
165_L168_H0.7491.00
56_L198_I0.7491.00
105_L116_I0.7481.00
148_V292_P0.7461.00
271_F295_I0.7421.00
220_L226_G0.7411.00
55_S250_I0.7401.00
187_A191_A0.7371.00
200_G209_T0.7361.00
362_A366_M0.7361.00
232_G240_T0.7351.00
260_R263_T0.7291.00
48_A51_Q0.7291.00
357_Q362_A0.7211.00
354_N357_Q0.7191.00
84_K87_T0.7171.00
194_D215_K0.7141.00
222_P226_G0.7131.00
46_Q50_N0.7121.00
52_I250_I0.7091.00
76_A174_H0.7081.00
56_L213_I0.7061.00
51_Q252_L0.7061.00
220_L228_S0.7041.00
292_P296_N0.7041.00
36_R312_E0.7001.00
295_I298_S0.6951.00
161_A173_F0.6931.00
147_H186_L0.6911.00
163_E166_K0.6881.00
218_T224_I0.6881.00
200_G205_G0.6871.00
275_L297_F0.6871.00
365_F369_L0.6861.00
233_I239_H0.6861.00
80_A195_L0.6861.00
185_P258_D0.6841.00
357_Q361_V0.6821.00
120_S135_A0.6811.00
102_T106_E0.6801.00
90_H138_P0.6791.00
95_S121_V0.6761.00
235_A238_E0.6751.00
262_D265_T0.6751.00
235_A239_H0.6741.00
160_A164_V0.6731.00
367_N370_R0.6711.00
267_V353_L0.6711.00
218_T222_P0.6691.00
203_F250_I0.6671.00
73_N197_S0.6661.00
246_L250_I0.6651.00
266_A269_E0.6651.00
271_F351_I0.6641.00
221_I224_I0.6641.00
220_L225_T0.6631.00
132_L164_V0.6631.00
235_A240_T0.6611.00
126_F156_Q0.6571.00
28_F31_P0.6541.00
276_S286_P0.6531.00
307_L310_M0.6531.00
156_Q161_A0.6481.00
40_E43_Q0.6471.00
143_V161_A0.6461.00
363_E366_M0.6451.00
236_G239_H0.6451.00
159_E186_L0.6431.00
115_D139_D0.6411.00
271_F366_M0.6411.00
138_P168_H0.6401.00
233_I238_E0.6391.00
209_T242_G0.6391.00
271_F275_L0.6391.00
99_P103_E0.6381.00
177_Y181_I0.6381.00
241_A244_V0.6321.00
76_A80_A0.6321.00
292_P297_F0.6271.00
192_G215_K0.6251.00
270_L273_N0.6241.00
284_N348_S0.6231.00
51_Q55_S0.6221.00
44_L47_A0.6221.00
91_I142_L0.6211.00
237_T240_T0.6211.00
158_I173_F0.6211.00
267_V271_F0.6191.00
278_I281_V0.6191.00
101_V105_L0.6161.00
204_H355_Y0.6151.00
234_R238_E0.6151.00
181_I186_L0.6141.00
158_I162_G0.6141.00
221_I227_G0.6141.00
223_L228_S0.6131.00
294_I298_S0.6131.00
234_R240_T0.6111.00
284_N296_N0.6081.00
222_P228_S0.6061.00
290_S295_I0.6061.00
122_N131_D0.6031.00
64_V75_A0.6011.00
37_Y317_F0.5981.00
93_A97_E0.5981.00
47_A50_N0.5971.00
365_F368_A0.5971.00
181_I203_F0.5971.00
55_S254_S0.5961.00
52_I65_F0.5951.00
209_T243_A0.5941.00
59_K62_D0.5931.00
69_A73_N0.5931.00
74_N107_Q0.5921.00
222_P227_G0.5921.00
307_L349_I0.5901.00
278_I366_M0.5891.00
93_A116_I0.5881.00
208_G211_A0.5851.00
147_H175_V0.5821.00
162_G193_I0.5811.00
222_P229_Q0.5811.00
232_G238_E0.5811.00
250_I254_S0.5801.00
249_A253_A0.5781.00
56_L188_I0.5771.00
66_T75_A0.5761.00
230_Q236_G0.5761.00
202_K205_G0.5741.00
368_A372_G0.5731.00
294_I297_F0.5711.00
302_I307_L0.5711.00
218_T223_L0.5711.00
242_G245_S0.5711.00
69_A197_S0.5691.00
258_D261_L0.5671.00
221_I229_Q0.5661.00
211_A214_V0.5661.00
89_K115_D0.5661.00
272_M287_Q0.5641.00
35_H38_G0.5631.00
281_V299_V0.5601.00
51_Q250_I0.5601.00
96_I121_V0.5571.00
78_K223_L0.5561.00
105_L144_S0.5561.00
33_S38_G0.5531.00
185_P261_L0.5531.00
270_L366_M0.5531.00
119_L127_V0.5531.00
304_A320_T0.5511.00
271_F274_R0.5501.00
252_L255_A0.5501.00
58_L213_I0.5491.00
297_F301_G0.5491.00
132_L136_I0.5491.00
264_M293_H0.5481.00
200_G203_F0.5481.00
219_R223_L0.5471.00
162_G175_V0.5421.00
291_A297_F0.5381.00
307_L311_L0.5371.00
274_R362_A0.5371.00
245_S249_A0.5361.00
178_V199_S0.5351.00
52_I181_I0.5351.00
218_T221_I0.5351.00
58_L196_C0.5341.00
267_V357_Q0.5341.00
293_H297_F0.5341.00
127_V132_L0.5271.00
290_S293_H0.5261.00
107_Q112_F0.5261.00
119_L132_L0.5251.00
304_A308_L0.5231.00
54_R57_G0.5231.00
257_F261_L0.5231.00
181_I250_I0.5211.00
177_Y180_G0.5211.00
298_S344_I0.5201.00
42_E46_Q0.5191.00
48_A243_A0.5181.00
50_N61_Y0.5181.00
231_K236_G0.5181.00
205_G209_T0.5171.00
194_D199_S0.5171.00
129_I157_P0.5161.00
180_G184_V0.5151.00
94_T145_M0.5141.00
46_Q49_K0.5141.00
291_A296_N0.5131.00
230_Q238_E0.5131.00
94_T97_E0.5131.00
219_R222_P0.5121.00
49_K230_Q0.5121.00
93_A144_S0.5111.00
236_G240_T0.5101.00
103_E107_Q0.5101.00
221_I226_G0.5101.00
247_A250_I0.5081.00
302_I306_V0.5071.00
230_Q234_R0.5061.00
202_K350_R0.5051.00
76_A212_L0.5041.00
220_L223_L0.5021.00
194_D197_S0.5011.00
196_C213_I0.5011.00
133_K136_I0.5001.00
232_G235_A0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4isyA 2 0.9764 100 0.339 Contact Map
1eg5A 2 0.9475 100 0.349 Contact Map
4hvkA 2 0.9685 100 0.353 Contact Map
3vaxA 2 0.916 100 0.361 Contact Map
3a9zA 2 0.9843 100 0.369 Contact Map
3lvmA 2 0.9764 100 0.372 Contact Map
3e9kA 2 0.9108 100 0.373 Contact Map
4ixoA 2 0.9318 100 0.375 Contact Map
1kmjA 2 0.9528 100 0.381 Contact Map
3caiA 2 0.9423 100 0.386 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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