ISCS2 - BACSU - GREMLIN Contacts

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ISCS2 - Putative cysteine desulfurase IscS 2

UniProt:	O34874	Sequences: MIYLDNSSTTKPYDEVLNVYEQTSSRYFGNPSSLHRYGAETEQLLQAAKNQIKRSLGLKKYDIVFTSGATEANNAALKGAALSKIKTGKHIIATSIEHPSVTESLEQLKELFGFDITYLSVNEDGFVSIEDLKAAIRPDTVLVSMMHVNNEVGSVQPIEAAGEVLKEHSNILFHVDYVQGIYKVPLAIEKAGIDLCSISGHKFHGLKGTGALIVKEGTRLIPLITGGSQQKGIRAGTEHTAGAVSLAKAINLASADFDTRLDTMTAVKELFMNRLSEIEGVVINTPQMNSAPHIINFSVPGIKAEVLLHMLEEQDIFVSTTSACSAKEHKPSKVLLEMGKGEQIAGSSIRISLNYSQTSDVAEPFMNALRPGIKKLREMMR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	381 (316)
Sequences:	34626 (24099)
Seq/√Len:	1355.7

ECOLI - ISCS CSDA SUFS SERC DCEB DCEA LTAE
BACSU - ISCS2 ISCS ISCS1 SUFS YCBU PUCG SERC
HALSA - CSD Q9HR11 Q9HMR6 MFNA Q9HHV3
SULSO - Q97WK1 Q97VM5 Q97ZK0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
270_L	363_E	3.463	1.00
274_R	366_M	3.412	1.00
90_H	117_T	3.370	1.00
162_G	173_F	3.335	1.00
270_L	359_S	3.244	1.00
157_P	160_A	3.038	1.00
147_H	180_G	2.865	1.00
90_H	137_R	2.716	1.00
187_A	190_K	2.665	1.00
51_Q	251_N	2.648	1.00
314_Q	371_P	2.605	1.00
133_K	167_E	2.602	1.00
133_K	164_V	2.598	1.00
90_H	115_D	2.572	1.00
95_S	120_S	2.522	1.00
177_Y	196_C	2.512	1.00
248_K	251_N	2.493	1.00
131_D	134_A	2.419	1.00
165_L	171_I	2.272	1.00
239_H	242_G	2.237	1.00
274_R	363_E	2.206	1.00
314_Q	368_A	2.188	1.00
278_I	370_R	2.188	1.00
198_I	211_A	2.177	1.00
270_L	274_R	2.163	1.00
90_H	140_T	2.157	1.00
143_V	165_L	2.120	1.00
131_D	135_A	2.044	1.00
364_P	368_A	1.978	1.00
274_R	277_E	1.930	1.00
351_I	365_F	1.921	1.00
163_E	167_E	1.891	1.00
162_G	166_K	1.889	1.00
128_S	131_D	1.878	1.00
282_V	298_S	1.858	1.00
73_N	100_S	1.857	1.00
57_G	187_A	1.851	1.00
266_A	359_S	1.842	1.00
159_E	163_E	1.811	1.00
90_H	139_D	1.810	1.00
310_M	372_G	1.804	1.00
159_E	190_K	1.800	1.00
117_T	135_A	1.785	1.00
168_H	171_I	1.783	1.00
67_S	71_E	1.769	1.00
165_L	173_F	1.764	1.00
263_T	267_V	1.750	1.00
272_M	295_I	1.744	1.00
303_K	306_V	1.712	1.00
120_S	131_D	1.698	1.00
89_K	141_V	1.696	1.00
129_I	164_V	1.638	1.00
119_L	135_A	1.632	1.00
269_E	273_N	1.622	1.00
284_N	298_S	1.610	1.00
89_K	139_D	1.601	1.00
282_V	300_P	1.594	1.00
145_M	161_A	1.586	1.00
54_R	254_S	1.567	1.00
164_V	168_H	1.566	1.00
309_H	313_E	1.560	1.00
160_A	163_E	1.556	1.00
274_R	370_R	1.553	1.00
166_K	194_D	1.548	1.00
122_N	126_F	1.535	1.00
66_T	72_A	1.517	1.00
51_Q	247_A	1.491	1.00
175_V	193_I	1.484	1.00
193_I	196_C	1.478	1.00
271_F	353_L	1.477	1.00
133_K	168_H	1.473	1.00
142_L	174_H	1.457	1.00
136_I	164_V	1.450	1.00
262_D	266_A	1.441	1.00
50_N	54_R	1.431	1.00
76_A	195_L	1.410	1.00
185_P	188_I	1.402	1.00
47_A	51_Q	1.385	1.00
258_D	262_D	1.377	1.00
166_K	192_G	1.371	1.00
359_S	363_E	1.371	1.00
162_G	192_G	1.362	1.00
145_M	173_F	1.359	1.00
186_L	190_K	1.355	1.00
296_N	348_S	1.352	1.00
224_I	227_G	1.349	1.00
129_I	160_A	1.344	1.00
265_T	269_E	1.337	1.00
69_A	178_V	1.331	1.00
200_G	246_L	1.328	1.00
62_D	216_E	1.325	1.00
358_T	361_V	1.324	1.00
310_M	313_E	1.320	1.00
206_L	245_S	1.312	1.00
148_V	291_A	1.298	1.00
80_A	172_L	1.298	1.00
130_E	133_K	1.297	1.00
261_L	265_T	1.293	1.00
185_P	189_E	1.287	1.00
277_E	370_R	1.285	1.00
95_S	118_Y	1.281	1.00
263_T	266_A	1.280	1.00
298_S	347_S	1.277	1.00
73_N	174_H	1.272	1.00
226_G	229_Q	1.268	1.00
310_M	314_Q	1.262	1.00
137_R	140_T	1.260	1.00
122_N	128_S	1.256	1.00
268_K	272_M	1.251	1.00
224_I	229_Q	1.251	1.00
158_I	175_V	1.251	1.00
51_Q	254_S	1.241	1.00
225_T	228_S	1.240	1.00
130_E	134_A	1.238	1.00
92_I	135_A	1.235	1.00
93_A	105_L	1.234	1.00
368_A	371_P	1.233	1.00
256_D	259_T	1.224	1.00
270_L	362_A	1.218	1.00
92_I	140_T	1.210	1.00
44_L	244_V	1.204	1.00
223_L	227_G	1.193	1.00
72_A	197_S	1.192	1.00
133_K	137_R	1.175	1.00
272_M	276_S	1.172	1.00
132_L	161_A	1.153	1.00
203_F	246_L	1.151	1.00
51_Q	54_R	1.149	1.00
164_V	167_E	1.145	1.00
221_I	225_T	1.141	1.00
223_L	226_G	1.141	1.00
50_N	53_K	1.136	1.00
52_I	211_A	1.136	1.00
247_A	251_N	1.134	1.00
48_A	247_A	1.126	1.00
271_F	362_A	1.120	1.00
59_K	216_E	1.113	1.00
174_H	197_S	1.112	1.00
107_Q	225_T	1.105	1.00
195_L	214_V	1.105	1.00
364_P	367_N	1.102	1.00
256_D	260_R	1.099	1.00
115_D	137_R	1.098	1.00
311_L	318_V	1.096	1.00
143_V	171_I	1.093	1.00
78_K	225_T	1.089	1.00
306_V	310_M	1.088	1.00
273_N	276_S	1.083	1.00
133_K	160_A	1.078	1.00
225_T	229_Q	1.076	1.00
186_L	189_E	1.074	1.00
276_S	287_Q	1.071	1.00
366_M	370_R	1.062	1.00
367_N	371_P	1.061	1.00
93_A	118_Y	1.056	1.00
43_Q	47_A	1.055	1.00
360_D	363_E	1.052	1.00
77_L	105_L	1.048	1.00
86_K	89_K	1.048	1.00
163_E	192_G	1.046	1.00
47_A	247_A	1.044	1.00
102_T	118_Y	1.040	1.00
85_I	88_G	1.032	1.00
85_I	114_F	1.028	1.00
124_D	286_P	1.023	1.00
103_E	106_E	1.022	1.00
129_I	133_K	1.017	1.00
274_R	367_N	1.013	1.00
291_A	294_I	1.005	1.00
363_E	367_N	1.004	1.00
318_V	349_I	1.003	1.00
267_V	362_A	1.003	1.00
204_H	354_N	1.002	1.00
147_H	196_C	0.988	1.00
59_K	189_E	0.988	1.00
132_L	143_V	0.986	1.00
55_S	181_I	0.983	1.00
148_V	180_G	0.983	1.00
137_R	168_H	0.981	1.00
316_I	364_P	0.981	1.00
145_M	158_I	0.980	1.00
307_L	372_G	0.976	1.00
181_I	198_I	0.974	1.00
316_I	368_A	0.972	1.00
185_P	190_K	0.965	1.00
273_N	277_E	0.964	1.00
280_G	300_P	0.964	1.00
259_T	262_D	0.959	1.00
251_N	255_A	0.958	1.00
91_I	105_L	0.946	1.00
166_K	173_F	0.943	1.00
259_T	263_T	0.935	1.00
144_S	174_H	0.932	1.00
57_G	189_E	0.932	1.00
212_L	215_K	0.930	1.00
88_G	141_V	0.929	1.00
251_N	254_S	0.929	1.00
222_P	225_T	0.920	1.00
291_A	295_I	0.916	1.00
147_H	158_I	0.913	1.00
275_L	369_L	0.910	1.00
308_L	320_T	0.910	1.00
307_L	369_L	0.909	1.00
63_I	213_I	0.904	1.00
155_V	184_V	0.903	1.00
188_I	213_I	0.902	1.00
61_Y	216_E	0.901	1.00
293_H	296_N	0.896	1.00
248_K	252_L	0.895	1.00
224_I	228_S	0.891	1.00
136_I	140_T	0.886	1.00
308_L	318_V	0.883	1.00
78_K	107_Q	0.882	1.00
220_L	224_I	0.881	1.00
136_I	168_H	0.880	1.00
316_I	365_F	0.880	1.00
96_I	156_Q	0.879	1.00
297_F	351_I	0.879	1.00
85_I	89_K	0.876	1.00
117_T	137_R	0.874	1.00
45_L	243_A	0.864	1.00
180_G	196_C	0.861	1.00
305_E	309_H	0.856	1.00
309_H	312_E	0.848	1.00
159_E	191_A	0.843	1.00
274_R	278_I	0.842	1.00
130_E	160_A	0.837	1.00
119_L	131_D	0.837	1.00
271_F	365_F	0.835	1.00
101_V	144_S	0.835	1.00
223_L	229_Q	0.828	1.00
146_M	150_N	0.826	1.00
165_L	170_N	0.825	1.00
74_N	78_K	0.821	1.00
174_H	195_L	0.818	1.00
272_M	283_I	0.816	1.00
53_K	58_L	0.814	1.00
267_V	359_S	0.808	1.00
147_H	191_A	0.807	1.00
268_K	295_I	0.806	1.00
260_R	355_Y	0.800	1.00
162_G	191_A	0.799	1.00
211_A	215_K	0.799	1.00
172_L	194_D	0.798	1.00
263_T	359_S	0.797	1.00
290_S	294_I	0.796	1.00
80_A	84_K	0.794	1.00
244_V	247_A	0.793	1.00
306_V	309_H	0.789	1.00
92_I	117_T	0.776	1.00
117_T	140_T	0.775	1.00
360_D	364_P	0.775	1.00
221_I	228_S	0.770	1.00
353_L	362_A	0.769	1.00
266_A	270_L	0.768	1.00
260_R	264_M	0.768	1.00
308_L	312_E	0.766	1.00
136_I	171_I	0.764	1.00
318_V	351_I	0.763	1.00
172_L	195_L	0.760	1.00
36_R	40_E	0.758	1.00
47_A	251_N	0.757	1.00
129_I	132_L	0.755	1.00
92_I	119_L	0.753	1.00
292_P	295_I	0.751	1.00
165_L	168_H	0.749	1.00
56_L	198_I	0.749	1.00
105_L	116_I	0.748	1.00
148_V	292_P	0.746	1.00
271_F	295_I	0.742	1.00
220_L	226_G	0.741	1.00
55_S	250_I	0.740	1.00
187_A	191_A	0.737	1.00
200_G	209_T	0.736	1.00
362_A	366_M	0.736	1.00
232_G	240_T	0.735	1.00
260_R	263_T	0.729	1.00
48_A	51_Q	0.729	1.00
357_Q	362_A	0.721	1.00
354_N	357_Q	0.719	1.00
84_K	87_T	0.717	1.00
194_D	215_K	0.714	1.00
222_P	226_G	0.713	1.00
46_Q	50_N	0.712	1.00
52_I	250_I	0.709	1.00
76_A	174_H	0.708	1.00
56_L	213_I	0.706	1.00
51_Q	252_L	0.706	1.00
220_L	228_S	0.704	1.00
292_P	296_N	0.704	1.00
36_R	312_E	0.700	1.00
295_I	298_S	0.695	1.00
161_A	173_F	0.693	1.00
147_H	186_L	0.691	1.00
163_E	166_K	0.688	1.00
218_T	224_I	0.688	1.00
200_G	205_G	0.687	1.00
275_L	297_F	0.687	1.00
365_F	369_L	0.686	1.00
233_I	239_H	0.686	1.00
80_A	195_L	0.686	1.00
185_P	258_D	0.684	1.00
357_Q	361_V	0.682	1.00
120_S	135_A	0.681	1.00
102_T	106_E	0.680	1.00
90_H	138_P	0.679	1.00
95_S	121_V	0.676	1.00
235_A	238_E	0.675	1.00
262_D	265_T	0.675	1.00
235_A	239_H	0.674	1.00
160_A	164_V	0.673	1.00
367_N	370_R	0.671	1.00
267_V	353_L	0.671	1.00
218_T	222_P	0.669	1.00
203_F	250_I	0.667	1.00
73_N	197_S	0.666	1.00
246_L	250_I	0.665	1.00
266_A	269_E	0.665	1.00
271_F	351_I	0.664	1.00
221_I	224_I	0.664	1.00
220_L	225_T	0.663	1.00
132_L	164_V	0.663	1.00
235_A	240_T	0.661	1.00
126_F	156_Q	0.657	1.00
28_F	31_P	0.654	1.00
276_S	286_P	0.653	1.00
307_L	310_M	0.653	1.00
156_Q	161_A	0.648	1.00
40_E	43_Q	0.647	1.00
143_V	161_A	0.646	1.00
363_E	366_M	0.645	1.00
236_G	239_H	0.645	1.00
159_E	186_L	0.643	1.00
115_D	139_D	0.641	1.00
271_F	366_M	0.641	1.00
138_P	168_H	0.640	1.00
233_I	238_E	0.639	1.00
209_T	242_G	0.639	1.00
271_F	275_L	0.639	1.00
99_P	103_E	0.638	1.00
177_Y	181_I	0.638	1.00
241_A	244_V	0.632	1.00
76_A	80_A	0.632	1.00
292_P	297_F	0.627	1.00
192_G	215_K	0.625	1.00
270_L	273_N	0.624	1.00
284_N	348_S	0.623	1.00
51_Q	55_S	0.622	1.00
44_L	47_A	0.622	1.00
91_I	142_L	0.621	1.00
237_T	240_T	0.621	1.00
158_I	173_F	0.621	1.00
267_V	271_F	0.619	1.00
278_I	281_V	0.619	1.00
101_V	105_L	0.616	1.00
204_H	355_Y	0.615	1.00
234_R	238_E	0.615	1.00
181_I	186_L	0.614	1.00
158_I	162_G	0.614	1.00
221_I	227_G	0.614	1.00
223_L	228_S	0.613	1.00
294_I	298_S	0.613	1.00
234_R	240_T	0.611	1.00
284_N	296_N	0.608	1.00
222_P	228_S	0.606	1.00
290_S	295_I	0.606	1.00
122_N	131_D	0.603	1.00
64_V	75_A	0.601	1.00
37_Y	317_F	0.598	1.00
93_A	97_E	0.598	1.00
47_A	50_N	0.597	1.00
365_F	368_A	0.597	1.00
181_I	203_F	0.597	1.00
55_S	254_S	0.596	1.00
52_I	65_F	0.595	1.00
209_T	243_A	0.594	1.00
59_K	62_D	0.593	1.00
69_A	73_N	0.593	1.00
74_N	107_Q	0.592	1.00
222_P	227_G	0.592	1.00
307_L	349_I	0.590	1.00
278_I	366_M	0.589	1.00
93_A	116_I	0.588	1.00
208_G	211_A	0.585	1.00
147_H	175_V	0.582	1.00
162_G	193_I	0.581	1.00
222_P	229_Q	0.581	1.00
232_G	238_E	0.581	1.00
250_I	254_S	0.580	1.00
249_A	253_A	0.578	1.00
56_L	188_I	0.577	1.00
66_T	75_A	0.576	1.00
230_Q	236_G	0.576	1.00
202_K	205_G	0.574	1.00
368_A	372_G	0.573	1.00
294_I	297_F	0.571	1.00
302_I	307_L	0.571	1.00
218_T	223_L	0.571	1.00
242_G	245_S	0.571	1.00
69_A	197_S	0.569	1.00
258_D	261_L	0.567	1.00
221_I	229_Q	0.566	1.00
211_A	214_V	0.566	1.00
89_K	115_D	0.566	1.00
272_M	287_Q	0.564	1.00
35_H	38_G	0.563	1.00
281_V	299_V	0.560	1.00
51_Q	250_I	0.560	1.00
96_I	121_V	0.557	1.00
78_K	223_L	0.556	1.00
105_L	144_S	0.556	1.00
33_S	38_G	0.553	1.00
185_P	261_L	0.553	1.00
270_L	366_M	0.553	1.00
119_L	127_V	0.553	1.00
304_A	320_T	0.551	1.00
271_F	274_R	0.550	1.00
252_L	255_A	0.550	1.00
58_L	213_I	0.549	1.00
297_F	301_G	0.549	1.00
132_L	136_I	0.549	1.00
264_M	293_H	0.548	1.00
200_G	203_F	0.548	1.00
219_R	223_L	0.547	1.00
162_G	175_V	0.542	1.00
291_A	297_F	0.538	1.00
307_L	311_L	0.537	1.00
274_R	362_A	0.537	1.00
245_S	249_A	0.536	1.00
178_V	199_S	0.535	1.00
52_I	181_I	0.535	1.00
218_T	221_I	0.535	1.00
58_L	196_C	0.534	1.00
267_V	357_Q	0.534	1.00
293_H	297_F	0.534	1.00
127_V	132_L	0.527	1.00
290_S	293_H	0.526	1.00
107_Q	112_F	0.526	1.00
119_L	132_L	0.525	1.00
304_A	308_L	0.523	1.00
54_R	57_G	0.523	1.00
257_F	261_L	0.523	1.00
181_I	250_I	0.521	1.00
177_Y	180_G	0.521	1.00
298_S	344_I	0.520	1.00
42_E	46_Q	0.519	1.00
48_A	243_A	0.518	1.00
50_N	61_Y	0.518	1.00
231_K	236_G	0.518	1.00
205_G	209_T	0.517	1.00
194_D	199_S	0.517	1.00
129_I	157_P	0.516	1.00
180_G	184_V	0.515	1.00
94_T	145_M	0.514	1.00
46_Q	49_K	0.514	1.00
291_A	296_N	0.513	1.00
230_Q	238_E	0.513	1.00
94_T	97_E	0.513	1.00
219_R	222_P	0.512	1.00
49_K	230_Q	0.512	1.00
93_A	144_S	0.511	1.00
236_G	240_T	0.510	1.00
103_E	107_Q	0.510	1.00
221_I	226_G	0.510	1.00
247_A	250_I	0.508	1.00
302_I	306_V	0.507	1.00
230_Q	234_R	0.506	1.00
202_K	350_R	0.505	1.00
76_A	212_L	0.504	1.00
220_L	223_L	0.502	1.00
194_D	197_S	0.501	1.00
196_C	213_I	0.501	1.00
133_K	136_I	0.500	1.00
232_G	235_A	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4isyA	2	0.9764	100	0.339	Contact Map
1eg5A	2	0.9475	100	0.349	Contact Map
4hvkA	2	0.9685	100	0.353	Contact Map
3vaxA	2	0.916	100	0.361	Contact Map
3a9zA	2	0.9843	100	0.369	Contact Map
3lvmA	2	0.9764	100	0.372	Contact Map
3e9kA	2	0.9108	100	0.373	Contact Map
4ixoA	2	0.9318	100	0.375	Contact Map
1kmjA	2	0.9528	100	0.381	Contact Map
3caiA	2	0.9423	100	0.386	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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