GREMLIN Database
PEL1 - Pectin lyase
UniProt: O34819 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 345 (291)
Sequences: 2386 (1512)
Seq/√Len: 88.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
162_V194_S5.2801.00
270_Y295_G3.7831.00
64_Y86_Q3.5951.00
121_T130_F3.4151.00
159_I162_V3.2141.00
151_L154_G3.0941.00
321_Q329_F2.6311.00
55_T127_N2.6081.00
182_I189_I2.6051.00
163_T336_S2.5281.00
193_N225_N2.5091.00
178_K181_Y2.4471.00
187_D220_H2.4391.00
102_N127_N2.4191.00
191_I194_S2.3641.00
75_L83_L2.3601.00
130_F133_L2.2901.00
182_I191_I2.2871.00
155_M187_D2.2481.00
81_K103_K2.1781.00
55_T102_N2.1531.00
326_T330_V2.1391.00
225_N321_Q2.0681.00
239_M244_F2.0651.00
77_G81_K2.0551.00
67_S70_D2.0461.00
295_G323_P1.9581.00
272_Y327_K1.9561.00
247_K320_V1.9481.00
121_T128_V1.9441.00
231_L235_G1.9331.00
227_Y252_N1.9301.00
224_A247_K1.9261.00
188_Y220_H1.9151.00
322_T326_T1.9081.00
63_V74_H1.8821.00
156_N188_Y1.8411.00
193_N224_A1.7971.00
256_Q259_T1.7681.00
323_P326_T1.7651.00
79_T102_N1.7651.00
135_F164_F1.7331.00
223_I226_N1.7031.00
163_T197_A1.6731.00
239_M266_I1.6521.00
180_V191_I1.6171.00
238_L261_A1.6051.00
121_T125_F1.5921.00
322_T325_Y1.5901.00
61_Q79_T1.5661.00
182_I186_A1.5641.00
224_A321_Q1.5591.00
58_K62_I1.5551.00
113_T136_E1.5531.00
134_T163_T1.5481.00
250_Y327_K1.5451.00
99_I119_L1.5211.00
274_G327_K1.5141.00
71_L97_V1.5121.00
258_I273_F1.5101.00
164_F196_F1.5011.00
293_D296_S1.4921.00
84_V108_S1.4841.00
193_N333_Y1.4730.99
71_L114_L1.4580.99
330_V334_A1.4510.99
309_S312_P1.4200.99
47_K50_S1.4050.99
62_I82_I1.4020.99
92_S115_K1.3900.99
252_N274_G1.3810.99
151_L182_I1.3770.99
51_K156_N1.3740.99
292_K295_G1.3570.99
245_H269_E1.3550.99
147_I177_D1.3460.99
49_I52_T1.3390.99
121_T151_L1.3360.99
101_S126_G1.3170.99
195_K334_A1.3140.99
73_T77_G1.3100.99
152_D183_G1.3060.99
205_L223_I1.3010.99
307_L310_W1.2980.99
199_H231_L1.2940.99
130_F149_L1.2930.99
77_G100_G1.2820.99
328_E332_K1.2810.99
58_K82_I1.2760.99
136_E165_S1.2740.99
135_F162_V1.2640.98
268_S296_S1.2640.98
135_F148_Q1.2630.98
250_Y326_T1.2610.98
180_V196_F1.2600.98
229_E252_N1.2530.98
195_K227_Y1.2510.98
203_L228_F1.2490.98
59_N62_I1.2460.98
249_N271_N1.2400.98
85_L89_L1.2380.98
329_F333_Y1.2370.98
163_T195_K1.2300.98
74_H83_L1.2270.98
88_D113_T1.2190.98
195_K229_E1.2120.98
46_E49_I1.2050.98
126_G155_M1.2030.98
68_V72_K1.1950.98
269_E294_I1.1850.98
48_G52_T1.1830.98
121_T157_Y1.1810.98
122_S152_D1.1710.97
193_N329_F1.1700.97
187_D217_G1.1640.97
202_G231_L1.1630.97
271_N296_S1.1630.97
227_Y250_Y1.1600.97
83_L97_V1.1590.97
164_F179_L1.1530.97
148_Q164_F1.1520.97
149_L164_F1.1480.97
47_K52_T1.1350.97
225_N247_K1.1220.97
133_L162_V1.1210.97
71_L75_L1.1200.97
71_L105_L1.1190.97
308_T312_P1.1090.96
47_K51_K1.1070.96
253_N276_G1.1050.96
119_L130_F1.1030.96
66_Q70_D1.0950.96
238_L259_T1.0860.96
56_G129_I1.0840.96
92_S116_N1.0840.96
186_A189_I1.0810.96
227_Y330_V1.0780.96
65_I74_H1.0750.96
68_V89_L1.0730.96
44_K49_I1.0710.96
260_I266_I1.0640.95
198_N230_N1.0600.95
165_S197_A1.0590.95
235_G251_S1.0530.95
207_Y211_S1.0460.95
151_L189_I1.0430.95
247_K318_Y1.0430.95
113_T134_T1.0420.95
284_D287_G1.0380.95
54_T156_N1.0290.95
270_Y323_P1.0280.95
151_L159_I1.0280.95
88_D112_K1.0230.94
154_G157_Y1.0120.94
137_H166_G1.0020.94
48_G51_K1.0000.94
177_D181_Y0.9940.93
258_I268_S0.9860.93
98_T122_S0.9850.93
297_Y324_E0.9830.93
227_Y331_T0.9690.93
246_V251_S0.9670.92
36_L39_F0.9510.92
164_F194_S0.9500.92
250_Y272_Y0.9490.92
46_E52_T0.9460.92
98_T120_T0.9390.91
216_D263_K0.9360.91
91_A117_I0.9360.91
78_S100_G0.9310.91
166_G200_K0.9300.91
245_H267_Y0.9290.91
184_K207_Y0.9270.91
282_I285_D0.9270.91
159_I180_V0.9270.91
183_G207_Y0.9210.91
128_V157_Y0.9210.91
149_L159_I0.9200.91
203_L223_I0.9180.90
250_Y274_G0.9170.90
230_N252_N0.9150.90
88_D115_K0.9140.90
194_S226_N0.9130.90
279_K282_I0.9130.90
327_K331_T0.9110.90
134_T336_S0.9060.90
115_K137_H0.9030.90
73_T76_G0.8990.89
83_L103_K0.8870.89
179_L231_L0.8850.89
74_H81_K0.8780.88
151_L157_Y0.8690.88
69_N73_T0.8660.88
72_K97_V0.8610.87
193_N321_Q0.8570.87
204_I240_R0.8560.87
297_Y323_P0.8540.87
247_K269_E0.8510.87
324_E328_E0.8510.87
61_Q82_I0.8510.87
89_L93_A0.8510.87
99_I128_V0.8490.87
47_K54_T0.8480.87
57_G60_G0.8470.86
207_Y210_D0.8420.86
281_G285_D0.8370.86
272_Y299_S0.8360.86
119_L135_F0.8300.85
164_F180_V0.8220.85
134_T165_S0.8200.85
84_V104_T0.8190.85
54_T57_G0.8170.84
325_Y329_F0.8150.84
200_K232_Y0.8140.84
206_G240_R0.8130.84
200_K233_V0.8120.84
45_N247_K0.8120.84
60_G82_I0.8120.84
201_Y232_Y0.8110.84
65_I70_D0.8090.84
79_T100_G0.8060.84
251_S271_N0.8060.84
75_L105_L0.8010.83
201_Y241_Y0.8000.83
269_E318_Y0.7960.83
75_L103_K0.7940.83
55_T59_N0.7920.83
307_L311_N0.7870.82
46_E50_S0.7770.81
272_Y330_V0.7750.81
270_Y320_V0.7740.81
310_W314_S0.7700.81
233_V256_Q0.7690.81
45_N58_K0.7660.80
123_S153_S0.7650.80
85_L93_A0.7530.79
99_I121_T0.7530.79
185_S218_Y0.7520.79
114_L119_L0.7520.79
220_H243_Y0.7510.79
160_D192_S0.7490.79
37_L41_G0.7460.79
158_W192_S0.7460.79
197_A229_E0.7430.78
226_N249_N0.7400.78
132_N337_Q0.7400.78
152_D184_K0.7390.78
126_G156_N0.7390.78
147_I178_K0.7380.78
233_V259_T0.7370.78
297_Y303_Q0.7370.78
272_Y326_T0.7350.78
177_D234_R0.7350.78
246_V258_I0.7330.77
149_L162_V0.7320.77
319_R324_E0.7300.77
222_T245_H0.7290.77
126_G157_Y0.7250.77
254_F276_G0.7250.77
63_V77_G0.7250.77
282_I286_K0.7250.77
100_G103_K0.7240.77
101_S123_S0.7220.76
45_N49_I0.7210.76
60_G103_K0.7210.76
132_N338_S0.7200.76
113_T133_L0.7190.76
87_N112_K0.7180.76
147_I181_Y0.7150.76
238_L244_F0.7140.76
321_Q325_Y0.7120.75
182_I221_M0.7110.75
75_L97_V0.7110.75
204_I209_D0.7090.75
59_N82_I0.7080.75
75_L100_G0.7040.75
76_G100_G0.7030.74
105_L130_F0.7010.74
325_Y328_E0.7010.74
232_Y255_N0.7010.74
64_Y84_V0.7000.74
56_G60_G0.7000.74
70_D73_T0.7000.74
66_Q86_Q0.6990.74
283_L287_G0.6980.74
259_T262_T0.6960.74
76_G97_V0.6920.73
272_Y323_P0.6920.73
223_I228_F0.6900.73
321_Q326_T0.6880.73
230_N253_N0.6850.73
311_N314_S0.6850.73
90_S115_K0.6840.72
68_V91_A0.6810.72
135_F151_L0.6770.72
93_A97_V0.6750.71
44_K47_K0.6720.71
48_G224_A0.6720.71
60_G81_K0.6720.71
189_I221_M0.6720.71
326_T331_T0.6710.71
101_S127_N0.6700.71
84_V106_V0.6650.70
138_S146_D0.6630.70
60_G75_L0.6610.70
211_S215_Y0.6600.70
300_L303_Q0.6600.70
76_G80_P0.6590.70
205_L208_P0.6570.69
223_I246_V0.6570.69
68_V94_K0.6570.69
211_S214_Q0.6490.68
129_I158_W0.6480.68
202_G228_F0.6470.68
296_S323_P0.6450.68
238_L256_Q0.6440.68
241_Y264_A0.6420.68
41_G59_N0.6410.68
85_L133_L0.6390.67
107_G133_L0.6330.67
144_N209_D0.6330.67
267_Y292_K0.6320.66
50_S188_Y0.6310.66
46_E51_K0.6310.66
308_T311_N0.6280.66
283_L286_K0.6270.66
71_L85_L0.6270.66
148_Q180_V0.6260.66
77_G102_N0.6240.65
289_G292_K0.6200.65
36_L41_G0.6190.65
181_Y240_R0.6190.65
187_D218_Y0.6170.65
128_V154_G0.6160.64
112_K133_L0.6120.64
265_K290_Y0.6120.64
184_K211_S0.6120.64
72_K93_A0.6110.64
280_G283_L0.6060.63
110_A113_T0.6060.63
108_S112_K0.6030.63
191_I196_F0.6020.63
307_L312_P0.6020.63
75_L81_K0.6020.63
114_L117_I0.6000.62
64_Y67_S0.6000.62
43_A49_I0.5990.62
75_L93_A0.5980.62
217_G241_Y0.5980.62
86_Q111_K0.5980.62
204_I208_P0.5950.62
81_K100_G0.5910.61
101_S128_V0.5870.61
245_H320_V0.5860.61
239_M246_V0.5810.60
281_G284_D0.5810.60
268_S273_F0.5790.60
312_P315_N0.5790.60
324_E332_K0.5790.60
270_Y322_T0.5760.59
59_N79_T0.5760.59
178_K199_H0.5750.59
177_D182_I0.5750.59
61_Q80_P0.5730.59
212_Q217_G0.5720.59
52_T188_Y0.5720.59
61_Q69_N0.5700.59
161_H193_N0.5670.58
149_L180_V0.5660.58
114_L133_L0.5630.58
112_K336_S0.5620.57
76_G98_T0.5600.57
180_V189_I0.5570.57
239_M264_A0.5570.57
260_I268_S0.5570.57
280_G291_F0.5560.57
267_Y315_N0.5540.56
320_V326_T0.5530.56
185_S240_R0.5520.56
119_L122_S0.5510.56
46_E82_I0.5500.56
219_P242_G0.5490.56
314_S317_S0.5490.56
156_N186_A0.5490.56
159_I191_I0.5480.56
215_Y218_Y0.5460.55
212_Q215_Y0.5420.55
317_S320_V0.5420.55
81_K271_N0.5420.55
50_S220_H0.5410.55
246_V271_N0.5400.54
155_M188_Y0.5380.54
289_G293_D0.5360.54
121_T159_I0.5340.54
56_G127_N0.5330.54
257_A261_A0.5330.54
153_S185_S0.5310.53
247_K321_Q0.5300.53
63_V73_T0.5300.53
235_G254_F0.5280.53
299_S303_Q0.5280.53
137_H165_S0.5280.53
105_L119_L0.5270.53
240_R264_A0.5250.52
60_G80_P0.5250.52
306_P310_W0.5240.52
225_N329_F0.5240.52
276_G279_K0.5220.52
177_D180_V0.5210.52
122_S154_G0.5200.52
167_H199_H0.5200.52
272_Y297_Y0.5200.52
244_F266_I0.5200.52
250_Y331_T0.5190.52
84_V91_A0.5190.52
51_K54_T0.5180.52
282_I287_G0.5180.52
49_I56_G0.5180.52
241_Y262_T0.5170.51
179_L199_H0.5140.51
202_G206_G0.5120.51
65_I85_L0.5110.51
36_L40_A0.5090.50
268_S271_N0.5080.50
279_K311_N0.5080.50
308_T314_S0.5070.50
54_T129_I0.5050.50
329_F332_K0.5040.50
102_N126_G0.5020.49
141_I144_N0.5020.49
116_N168_S0.5020.49
219_P241_Y0.5020.49
239_M258_I0.5000.49
239_M268_S0.5000.49
317_S324_E0.5000.49
242_G264_A0.5000.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1vblA 1 0.8638 100 0.239 Contact Map
1pe9A 1 0.8783 100 0.245 Contact Map
2o04A 1 0.8638 100 0.247 Contact Map
4hwvA 2 0.9217 100 0.253 Contact Map
1pclA 1 0.858 100 0.272 Contact Map
2qy1A 1 0.8377 100 0.292 Contact Map
1pxzA 1 0.8725 100 0.295 Contact Map
3vmvA 1 0.8261 100 0.297 Contact Map
3zscA 3 0.8435 100 0.299 Contact Map
1airA 1 0.8493 100 0.331 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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