GREMLIN Database
PKSB - Probable polyketide biosynthesis zinc-dependent hydrolase PksB
UniProt: O34769 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 225 (180)
Sequences: 14954 (10355)
Seq/√Len: 771.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
31_S56_K4.0641.00
120_T129_C3.7851.00
107_T114_R3.0051.00
60_L71_V2.9381.00
109_S114_R2.6821.00
57_A83_Y2.5771.00
107_T116_Q2.5431.00
115_A132_F2.4751.00
59_A130_Y2.4261.00
117_C128_M2.4031.00
116_Q133_S2.3131.00
86_K103_D2.2261.00
33_I57_A2.2191.00
31_S54_E2.1981.00
45_T77_M2.1981.00
83_Y100_I2.1391.00
32_A53_A1.9561.00
81_Q100_I1.8981.00
35_V137_F1.8881.00
47_T51_L1.8881.00
132_F135_S1.8561.00
117_C130_Y1.8101.00
25_E53_A1.8031.00
57_A81_Q1.8021.00
88_E126_G1.7741.00
156_S159_E1.7611.00
104_D166_R1.7391.00
85_S119_L1.7351.00
83_Y102_L1.7261.00
22_Y178_Y1.7051.00
131_L175_V1.6371.00
103_D106_Q1.6311.00
104_D167_I1.6261.00
162_D166_R1.6131.00
25_E32_A1.5831.00
48_L55_L1.5301.00
73_P77_M1.5291.00
55_L80_A1.5101.00
71_V82_V1.4801.00
45_T78_F1.4741.00
47_T50_E1.4601.00
46_T50_E1.4421.00
116_Q131_L1.4341.00
45_T49_S1.4191.00
168_K193_D1.4121.00
85_S128_M1.4081.00
33_I59_A1.3871.00
35_V61_T1.3831.00
113_T132_F1.3751.00
129_C136_I1.3561.00
100_I106_Q1.3561.00
27_I30_R1.3331.00
72_D76_K1.3291.00
131_L136_I1.3281.00
187_P190_S1.2861.00
161_F165_Q1.2741.00
118_L131_L1.2671.00
72_D75_T1.2301.00
122_G163_S1.2261.00
42_S46_T1.2201.00
49_S77_M1.2131.00
120_T163_S1.1941.00
86_K101_S1.1931.00
41_L73_P1.1791.00
55_L78_F1.1721.00
165_Q196_Q1.1701.00
75_T82_V1.1701.00
125_A159_E1.1561.00
46_T49_S1.1541.00
22_Y137_F1.1431.00
166_R170_E1.1411.00
34_V55_L1.1401.00
149_I152_D1.1371.00
124_T127_G1.1171.00
32_A55_L1.1021.00
75_T99_L1.0971.00
104_D119_L1.0801.00
177_V185_K1.0781.00
89_I93_Q1.0681.00
129_C141_T1.0661.00
89_I101_S1.0641.00
136_I171_V1.0641.00
26_D112_N1.0561.00
23_I47_T1.0561.00
118_L167_I1.0511.00
41_L70_L1.0451.00
37_P58_V1.0321.00
159_E162_D1.0321.00
176_R184_G1.0181.00
157_A161_F1.0171.00
120_T141_T1.0121.00
73_P76_K1.0071.00
59_A115_A0.9981.00
109_S112_N0.9941.00
172_S175_V0.9901.00
127_G139_G0.9761.00
26_D111_G0.9651.00
87_K119_L0.9621.00
61_T139_G0.9621.00
105_H116_Q0.9561.00
33_I110_I0.9531.00
165_Q168_K0.9521.00
12_T47_T0.9421.00
113_T135_S0.9381.00
43_K47_T0.9331.00
150_C157_A0.9321.00
191_I195_Y0.9311.00
41_L45_T0.9251.00
168_K196_Q0.9251.00
190_S194_L0.9221.00
157_A160_M0.9191.00
158_E162_D0.9151.00
40_E43_K0.9101.00
65_Y69_N0.9081.00
102_L128_M0.9031.00
60_L82_V0.8891.00
136_I177_V0.8731.00
167_I170_E0.8711.00
102_L117_C0.8691.00
173_P195_Y0.8671.00
110_I113_T0.8581.00
24_I35_V0.8471.00
30_R52_E0.8421.00
191_I194_L0.8341.00
25_E47_T0.8331.00
150_C160_M0.8301.00
138_T177_V0.8241.00
58_V80_A0.8241.00
21_C44_I0.8211.00
145_E188_G0.8141.00
31_S57_A0.8131.00
71_V75_T0.8091.00
87_K90_D0.8021.00
94_F98_N0.7981.00
19_N38_S0.7961.00
63_S84_M0.7951.00
44_I48_L0.7931.00
142_V177_V0.7871.00
84_M92_Y0.7871.00
189_H193_D0.7861.00
84_M98_N0.7841.00
131_L171_V0.7831.00
141_T163_S0.7821.00
94_F99_L0.7811.00
17_W65_Y0.7721.00
26_D30_R0.7691.00
120_T127_G0.7691.00
144_T183_F0.7691.00
141_T167_I0.7601.00
63_S88_E0.7591.00
141_T160_M0.7581.00
102_L130_Y0.7581.00
34_V37_P0.7561.00
86_K89_I0.7551.00
190_S193_D0.7541.00
57_A100_I0.7531.00
23_I34_V0.7531.00
116_Q171_V0.7511.00
25_E30_R0.7501.00
192_K196_Q0.7441.00
88_E91_Y0.7431.00
127_G141_T0.7411.00
84_M88_E0.7391.00
118_L129_C0.7391.00
173_P191_I0.7341.00
114_R133_S0.7311.00
88_E124_T0.7281.00
40_E44_I0.7271.00
149_I153_D0.7251.00
138_T179_P0.7251.00
25_E51_L0.7211.00
23_I32_A0.7201.00
119_L128_M0.7171.00
42_S73_P0.7171.00
33_I115_A0.7151.00
145_E189_H0.7141.00
173_P188_G0.7061.00
107_T133_S0.7061.00
125_A154_G0.7041.00
58_V74_L0.7021.00
34_V74_L0.6941.00
31_S111_G0.6901.00
143_F148_G0.6861.00
21_C38_S0.6791.00
58_V82_V0.6781.00
87_K103_D0.6761.00
19_N180_G0.6761.00
145_E148_G0.6731.00
56_K81_Q0.6721.00
93_Q97_R0.6651.00
115_A130_Y0.6651.00
164_I168_K0.6551.00
13_R18_T0.6511.00
44_I47_T0.6501.00
26_D113_T0.6481.00
102_L108_I0.6461.00
60_L68_V0.6441.00
26_D31_S0.6401.00
178_Y184_G0.6391.00
118_L166_R0.6361.00
81_Q99_L0.6331.00
43_K46_T0.6321.00
15_Q21_C0.6291.00
34_V48_L0.6281.00
135_S178_Y0.6251.00
108_I115_A0.6231.00
35_V59_A0.6201.00
74_L78_F0.6191.00
165_Q170_E0.6161.00
160_M164_I0.6111.00
129_C167_I0.6101.00
58_V71_V0.6051.00
102_L106_Q0.6051.00
41_L44_I0.6031.00
32_A54_E0.5961.00
32_A48_L0.5941.00
37_P71_V0.5931.00
32_A56_K0.5881.00
70_L73_P0.5871.00
42_S45_T0.5861.00
192_K195_Y0.5851.00
63_S126_G0.5851.00
137_F178_Y0.5841.00
23_I55_L0.5841.00
103_D119_L0.5821.00
24_I33_I0.5821.00
162_D165_Q0.5821.00
146_G149_I0.5801.00
173_P192_K0.5791.00
98_N101_S0.5791.00
30_R54_E0.5771.00
20_Y182_S0.5751.00
131_L173_P0.5721.00
20_Y180_G0.5691.00
76_K94_F0.5651.00
22_Y180_G0.5641.00
24_I132_F0.5571.00
35_V130_Y0.5561.00
23_I48_L0.5561.00
122_G150_C0.5551.00
129_C138_T0.5471.00
121_P163_S0.5471.00
110_I115_A0.5411.00
49_S52_E0.5401.00
147_C150_C0.5391.00
13_R20_Y0.5361.00
176_R186_S0.5351.00
147_C164_I0.5341.00
89_I92_Y0.5331.00
147_C157_A0.5321.00
48_L78_F0.5321.00
190_S196_Q0.5321.00
14_Y40_E0.5281.00
106_Q117_C0.5221.00
140_D181_H0.5211.00
83_Y106_Q0.5201.00
159_E163_S0.5191.00
75_T79_N0.5191.00
14_Y19_N0.5181.00
20_Y183_F0.5151.00
151_E155_S0.5141.00
131_L172_S0.5141.00
135_S176_R0.5121.00
177_V187_P0.5101.00
48_L53_A0.5091.00
42_S77_M0.5091.00
138_T185_K0.5051.00
85_S88_E0.5051.00
59_A108_I0.5021.00
147_C160_M0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4yslA 2 0.9956 100 0.318 Contact Map
3r2uA 4 0.9867 100 0.328 Contact Map
4efzA 2 0.9867 100 0.333 Contact Map
2zo4A 1 0.9689 100 0.335 Contact Map
3tp9A 2 0.9689 100 0.336 Contact Map
2p18A 1 0.9511 100 0.34 Contact Map
1qh5A 1 0.9244 100 0.343 Contact Map
2qedA 1 0.9244 100 0.349 Contact Map
4ysbA 2 0.9422 100 0.357 Contact Map
1xm8A 1 0.9378 100 0.359 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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