YLBJ - BACSU - GREMLIN Contacts

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YLBJ - Sporulation integral membrane protein YlbJ

UniProt:	O34765	Sequences: MNLSKINTLLIASFFLFLTATVISHPQASFEASKTGLSMWWEVVFPSLLPFFILSELLIGFGIVRFVGVLLEPFMRPIFRVPGVGGFVLAMGMASGNPAGAKLTARLRQENQISRVEAERLASFTNSSNPLFIFGAVAVGFFQNASLGILLASAHYLGNLAVGLTMRSYGRKEEQHLRRKKTIPFPSVKDALHALHTARLAEKRPLGKILGDAVTSSVQTLLMVGGFIILFSVFNRLLSVVQLTDVLSVFTKGALTLFQLPTQLDIPLLSGMFEITLGSKLVSETDVSLLQKAIIVSFILGFSGFSVQAQVAGILSETDIRFKPFFIARLLQGVYAAVFVMLLWKPLYTAWHDPYQYVFKSLNSSDMSTAFINGWNLLVQIGPAVTLCALFTYIIIFSYRLTNGTKKG	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	408 (361)
Sequences:	547 (481)
Seq/√Len:	25.3

BACSU - YLBJ

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
49_L	53_I	9.308	1.00
161_A	339_F	6.682	1.00
108_R	319_D	6.015	1.00
142_F	296_V	5.719	1.00
298_F	333_G	5.133	1.00
40_W	225_G	4.764	1.00
44_V	228_I	4.701	1.00
92_G	126_N	4.198	1.00
88_V	103_L	3.972	1.00
115_R	321_R	3.747	1.00
133_I	151_L	3.377	1.00
81_V	85_G	3.271	1.00
109_Q	317_E	3.009	1.00
325_F	329_R	2.985	1.00
302_F	308_Q	2.790	0.99
115_R	319_D	2.722	0.99
308_Q	322_F	2.719	0.99
82_P	117_E	2.714	0.99
22_V	29_S	2.662	0.99
165_T	335_Y	2.615	0.99
128_S	155_H	2.545	0.99
108_R	118_A	2.545	0.99
40_W	45_F	2.416	0.98
105_A	318_T	2.296	0.98
157_L	339_F	2.272	0.98
60_G	209_I	2.252	0.98
108_R	115_R	2.223	0.97
138_A	148_G	2.146	0.97
126_N	275_I	2.060	0.96
157_L	161_A	2.060	0.96
93_M	159_N	2.041	0.96
68_G	83_G	2.040	0.96
356_Q	359_F	2.038	0.96
122_A	315_L	2.032	0.95
341_M	345_K	1.994	0.95
97_N	325_F	1.947	0.94
159_N	329_R	1.944	0.94
27_Q	31_E	1.877	0.93
218_V	222_L	1.820	0.92
192_L	197_T	1.819	0.92
40_W	226_G	1.791	0.91
126_N	329_R	1.733	0.90
97_N	301_G	1.729	0.90
107_L	113_I	1.724	0.90
161_A	335_Y	1.708	0.89
301_G	333_G	1.703	0.89
71_L	90_A	1.680	0.88
36_G	232_S	1.657	0.88
40_W	95_S	1.649	0.87
374_G	378_L	1.636	0.87
368_S	378_L	1.619	0.86
158_G	336_A	1.613	0.86
85_G	121_L	1.581	0.85
99_A	129_N	1.580	0.85
29_S	32_A	1.565	0.84
47_S	128_S	1.565	0.84
371_F	377_L	1.549	0.84
129_N	329_R	1.548	0.84
67_V	123_S	1.539	0.83
132_F	276_T	1.536	0.83
254_A	258_F	1.534	0.83
271_G	306_S	1.502	0.82
86_G	216_S	1.501	0.82
360_K	375_W	1.497	0.82
39_M	275_I	1.486	0.81
160_L	295_I	1.485	0.81
22_V	151_L	1.471	0.80
141_F	279_S	1.464	0.80
150_L	343_L	1.457	0.80
49_L	228_I	1.447	0.79
65_R	198_A	1.443	0.79
156_Y	309_A	1.441	0.79
350_A	353_D	1.436	0.79
7_N	22_V	1.431	0.79
97_N	126_N	1.414	0.78
49_L	301_G	1.393	0.77
303_S	326_F	1.384	0.76
137_V	198_A	1.369	0.75
327_I	331_L	1.366	0.75
119_E	311_V	1.350	0.74
260_L	295_I	1.341	0.74
375_W	380_Q	1.335	0.73
251_T	375_W	1.325	0.73
114_S	117_E	1.322	0.73
378_L	384_A	1.314	0.72
294_I	337_A	1.301	0.72
108_R	318_T	1.298	0.71
93_M	127_S	1.293	0.71
74_F	344_W	1.291	0.71
151_L	297_S	1.291	0.71
371_F	379_V	1.287	0.71
295_I	305_F	1.285	0.71
299_I	332_Q	1.283	0.70
39_M	126_N	1.279	0.70
171_R	268_L	1.256	0.69
158_G	301_G	1.248	0.68
298_F	337_A	1.245	0.68
33_S	40_W	1.239	0.68
58_L	207_G	1.236	0.67
372_I	379_V	1.233	0.67
255_L	268_L	1.225	0.67
275_I	283_S	1.210	0.66
94_A	302_F	1.208	0.66
33_S	36_G	1.177	0.64
293_A	340_V	1.176	0.64
97_N	127_S	1.175	0.63
247_L	257_L	1.161	0.63
246_V	249_V	1.157	0.62
71_L	210_L	1.156	0.62
357_Y	360_K	1.151	0.62
246_V	256_T	1.149	0.62
72_E	76_R	1.145	0.61
87_F	276_T	1.122	0.60
157_L	303_S	1.120	0.60
151_L	333_G	1.118	0.60
133_I	155_H	1.113	0.59
106_R	110_E	1.111	0.59
335_Y	339_F	1.110	0.59
352_H	377_L	1.104	0.59
375_W	378_L	1.102	0.58
379_V	384_A	1.099	0.58
23_I	347_L	1.085	0.57
268_L	272_M	1.084	0.57
372_I	378_L	1.084	0.57
330_L	334_V	1.083	0.57
376_N	379_V	1.079	0.57
80_R	299_I	1.078	0.57
33_S	233_V	1.070	0.56
77_P	167_R	1.069	0.56
293_A	344_W	1.068	0.56
47_S	127_S	1.063	0.56
155_H	332_Q	1.048	0.55
280_K	284_E	1.047	0.54
211_G	225_G	1.046	0.54
131_L	233_V	1.035	0.54
187_S	190_D	1.032	0.53
79_F	83_G	1.029	0.53
336_A	339_F	1.024	0.53
158_G	305_F	1.020	0.53
32_A	223_M	1.018	0.52
245_D	249_V	1.018	0.52
158_G	332_Q	1.014	0.52
77_P	142_F	1.014	0.52
128_S	238_L	1.012	0.52
279_S	296_V	1.011	0.52
158_G	335_Y	1.008	0.52
216_S	300_L	1.005	0.51
105_A	315_L	1.005	0.51
77_P	119_E	1.004	0.51
251_T	268_L	0.999	0.51
358_V	362_L	0.997	0.51
378_L	382_G	0.996	0.51
52_F	217_S	0.995	0.51
133_I	156_Y	0.991	0.50
372_I	375_W	0.991	0.50
249_V	253_G	0.991	0.50
36_G	227_F	0.990	0.50
368_S	377_L	0.987	0.50
44_V	59_I	0.983	0.50
129_N	301_G	0.981	0.50
19_T	250_F	0.977	0.49
26_P	36_G	0.976	0.49
158_G	340_V	0.973	0.49
360_K	381_I	0.972	0.49
301_G	336_A	0.970	0.49
372_I	381_I	0.969	0.49
124_F	167_R	0.969	0.49
283_S	320_I	0.962	0.48
95_S	276_T	0.962	0.48
85_G	92_G	0.959	0.48
120_R	163_G	0.958	0.48
201_A	234_F	0.957	0.48
300_L	308_Q	0.954	0.48
369_T	373_N	0.952	0.48
362_L	372_I	0.949	0.47
144_N	292_K	0.949	0.47
154_A	303_S	0.940	0.47
126_N	325_F	0.939	0.47
91_M	163_G	0.939	0.47
227_F	307_V	0.938	0.47
374_G	384_A	0.936	0.46
40_W	44_V	0.934	0.46
52_F	125_T	0.933	0.46
370_A	384_A	0.931	0.46
102_K	276_T	0.929	0.46
213_A	269_L	0.926	0.46
374_G	380_Q	0.925	0.46
337_A	341_M	0.925	0.46
59_I	222_L	0.924	0.46
17_F	268_L	0.924	0.46
88_V	104_T	0.917	0.45
371_F	374_G	0.916	0.45
134_F	138_A	0.914	0.45
374_G	377_L	0.911	0.45
357_Y	362_L	0.910	0.45
369_T	378_L	0.907	0.44
97_N	307_V	0.903	0.44
30_F	314_I	0.902	0.44
40_W	211_G	0.902	0.44
148_G	227_F	0.901	0.44
127_S	159_N	0.901	0.44
136_A	141_F	0.900	0.44
311_V	329_R	0.898	0.44
215_T	219_Q	0.898	0.44
120_R	227_F	0.893	0.43
85_G	314_I	0.893	0.43
104_T	318_T	0.891	0.43
75_M	79_F	0.891	0.43
125_T	207_G	0.886	0.43
369_T	376_N	0.886	0.43
197_T	250_F	0.884	0.43
104_T	125_T	0.883	0.43
373_N	376_N	0.880	0.42
205_P	329_R	0.878	0.42
373_N	380_Q	0.876	0.42
359_F	362_L	0.875	0.42
121_L	271_G	0.874	0.42
191_A	195_L	0.874	0.42
373_N	377_L	0.870	0.42
40_W	117_E	0.868	0.42
377_L	380_Q	0.867	0.41
350_A	354_P	0.867	0.41
143_Q	146_S	0.865	0.41
119_E	125_T	0.861	0.41
358_V	361_S	0.857	0.41
360_K	371_F	0.855	0.41
78_I	306_S	0.854	0.41
252_K	362_L	0.853	0.40
190_D	194_A	0.852	0.40
150_L	164_L	0.851	0.40
33_S	225_G	0.851	0.40
71_L	144_N	0.850	0.40
203_K	209_I	0.848	0.40
80_R	171_R	0.848	0.40
56_E	125_T	0.848	0.40
131_L	224_V	0.847	0.40
269_L	342_L	0.847	0.40
208_K	212_D	0.845	0.40
45_F	218_V	0.843	0.40
65_R	112_Q	0.841	0.40
252_K	358_V	0.840	0.40
297_S	336_A	0.838	0.39
102_K	220_T	0.835	0.39
43_V	136_A	0.834	0.39
147_L	293_A	0.833	0.39
124_F	163_G	0.832	0.39
297_S	340_V	0.831	0.39
125_T	329_R	0.829	0.39
88_V	329_R	0.827	0.39
351_W	362_L	0.827	0.39
259_Q	288_S	0.827	0.39
77_P	87_F	0.826	0.39
229_I	270_S	0.823	0.38
40_W	87_F	0.823	0.38
151_L	300_L	0.816	0.38
127_S	235_N	0.814	0.38
211_G	227_F	0.813	0.38
70_L	145_A	0.806	0.37
138_A	169_Y	0.805	0.37
63_I	78_I	0.805	0.37
95_S	329_R	0.804	0.37
97_N	129_N	0.803	0.37
376_N	381_I	0.802	0.37
141_F	322_F	0.798	0.37
52_F	155_H	0.798	0.37
247_L	354_P	0.795	0.36
373_N	378_L	0.795	0.36
357_Y	372_I	0.794	0.36
369_T	372_I	0.793	0.36
91_M	103_L	0.792	0.36
351_W	358_V	0.791	0.36
339_F	343_L	0.790	0.36
19_T	214_V	0.790	0.36
154_A	340_V	0.783	0.36
198_A	202_E	0.782	0.36
208_K	221_L	0.780	0.35
234_F	256_T	0.778	0.35
71_L	344_W	0.777	0.35
206_L	331_L	0.775	0.35
368_S	381_I	0.774	0.35
127_S	300_L	0.774	0.35
196_H	200_L	0.773	0.35
206_L	210_L	0.771	0.35
86_G	127_S	0.768	0.35
71_L	332_Q	0.763	0.34
305_F	377_L	0.762	0.34
145_A	229_I	0.759	0.34
377_L	384_A	0.759	0.34
129_N	302_F	0.753	0.34
282_V	306_S	0.750	0.33
100_G	276_T	0.749	0.33
64_V	156_Y	0.746	0.33
258_F	359_F	0.744	0.33
369_T	379_V	0.744	0.33
334_V	338_V	0.743	0.33
136_A	232_S	0.743	0.33
115_R	119_E	0.742	0.33
97_N	214_V	0.740	0.33
207_G	226_G	0.739	0.33
47_S	150_L	0.738	0.33
57_L	147_L	0.737	0.33
192_L	247_L	0.737	0.33
375_W	379_V	0.737	0.33
120_R	297_S	0.735	0.33
106_R	109_Q	0.735	0.33
97_N	375_W	0.735	0.33
133_I	162_V	0.734	0.32
127_S	329_R	0.727	0.32
11_I	14_F	0.726	0.32
126_N	307_V	0.724	0.32
44_V	275_I	0.723	0.32
91_M	147_L	0.722	0.32
99_A	221_L	0.719	0.31
52_F	77_P	0.716	0.31
134_F	137_V	0.715	0.31
352_H	369_T	0.714	0.31
32_A	135_G	0.711	0.31
18_L	260_L	0.709	0.31
362_L	379_V	0.709	0.31
271_G	332_Q	0.709	0.31
50_P	117_E	0.708	0.31
44_V	235_N	0.705	0.31
273_F	279_S	0.705	0.31
16_L	281_L	0.705	0.31
138_A	156_Y	0.702	0.30
86_G	344_W	0.701	0.30
138_A	151_L	0.700	0.30
175_Q	268_L	0.698	0.30
136_A	140_G	0.697	0.30
301_G	328_A	0.695	0.30
66_F	87_F	0.692	0.30
368_S	371_F	0.691	0.30
359_F	375_W	0.691	0.30
7_N	100_G	0.691	0.30
104_T	118_A	0.688	0.30
245_D	248_S	0.685	0.29
230_L	322_F	0.684	0.29
204_R	212_D	0.683	0.29
306_S	332_Q	0.683	0.29
356_Q	370_A	0.682	0.29
79_F	124_F	0.678	0.29
15_F	214_V	0.678	0.29
99_A	325_F	0.675	0.29
25_H	28_A	0.674	0.29
56_E	211_G	0.673	0.29
371_F	380_Q	0.664	0.28
88_V	122_A	0.664	0.28
305_F	337_A	0.663	0.28
164_L	351_W	0.662	0.28
94_A	102_K	0.661	0.28
128_S	133_I	0.657	0.28
11_I	22_V	0.655	0.28
26_P	33_S	0.651	0.27
85_G	117_E	0.651	0.27
49_L	275_I	0.650	0.27
370_A	376_N	0.649	0.27
81_V	279_S	0.648	0.27
245_D	360_K	0.646	0.27
190_D	193_H	0.644	0.27
21_T	237_L	0.644	0.27
353_D	360_K	0.643	0.27
97_N	163_G	0.643	0.27
251_T	357_Y	0.643	0.27
116_V	170_G	0.641	0.27
120_R	148_G	0.641	0.27
356_Q	379_V	0.640	0.27
36_G	225_G	0.640	0.27
77_P	124_F	0.638	0.27
360_K	370_A	0.637	0.27
17_F	21_T	0.635	0.26
13_S	380_Q	0.634	0.26
94_A	301_G	0.633	0.26
354_P	377_L	0.633	0.26
76_R	83_G	0.633	0.26
250_F	256_T	0.633	0.26
89_L	160_L	0.633	0.26
272_M	305_F	0.630	0.26
120_R	271_G	0.629	0.26
52_F	75_M	0.629	0.26
28_A	210_L	0.629	0.26
374_G	382_G	0.628	0.26
213_A	306_S	0.627	0.26
43_V	97_N	0.627	0.26
142_F	255_L	0.627	0.26
75_M	86_G	0.625	0.26
10_L	70_L	0.623	0.26
368_S	372_I	0.622	0.26
148_G	297_S	0.620	0.26
277_L	344_W	0.619	0.26
245_D	368_S	0.617	0.25
245_D	370_A	0.617	0.25
377_L	381_I	0.616	0.25
222_L	342_L	0.616	0.25
170_G	238_L	0.614	0.25
372_I	383_P	0.614	0.25
59_I	342_L	0.614	0.25
247_L	258_F	0.613	0.25
294_I	334_V	0.613	0.25
174_E	272_M	0.612	0.25
249_V	258_F	0.611	0.25
129_N	308_Q	0.610	0.25
253_G	257_L	0.610	0.25
284_E	338_V	0.610	0.25
91_M	125_T	0.609	0.25
29_S	292_K	0.609	0.25
141_F	300_L	0.608	0.25
88_V	280_K	0.608	0.25
246_V	251_T	0.605	0.25
254_A	356_Q	0.605	0.25
111_N	245_D	0.604	0.25
164_L	335_Y	0.604	0.25
154_A	296_V	0.603	0.25
162_V	222_L	0.601	0.25
92_G	121_L	0.601	0.25
15_F	192_L	0.600	0.25
220_T	228_I	0.599	0.24
99_A	127_S	0.599	0.24
64_V	121_L	0.598	0.24
155_H	340_V	0.598	0.24
78_I	334_V	0.597	0.24
119_E	321_R	0.597	0.24
8_T	11_I	0.596	0.24
88_V	159_N	0.596	0.24
14_F	345_K	0.594	0.24
360_K	372_I	0.593	0.24
20_A	362_L	0.592	0.24
87_F	147_L	0.592	0.24
100_G	129_N	0.591	0.24
187_S	193_H	0.591	0.24
47_S	294_I	0.588	0.24
129_N	159_N	0.588	0.24
158_G	217_S	0.587	0.24
257_L	342_L	0.587	0.24
75_M	83_G	0.586	0.24
373_N	383_P	0.585	0.24
14_F	268_L	0.584	0.24
265_D	269_L	0.584	0.24
92_G	279_S	0.582	0.24
135_G	218_V	0.582	0.24
95_S	314_I	0.582	0.24
362_L	381_I	0.580	0.23
137_V	296_V	0.580	0.23
217_S	226_G	0.579	0.23
81_V	128_S	0.579	0.23
32_A	36_G	0.578	0.23
187_S	371_F	0.577	0.23
89_L	93_M	0.577	0.23
173_E	224_V	0.576	0.23
148_G	211_G	0.575	0.23
82_P	91_M	0.574	0.23
40_W	50_P	0.573	0.23
351_W	359_F	0.571	0.23
127_S	325_F	0.570	0.23
192_L	336_A	0.569	0.23
124_F	315_L	0.569	0.23
210_L	230_L	0.565	0.23
30_F	210_L	0.564	0.23
354_P	369_T	0.564	0.23
129_N	141_F	0.563	0.23
40_W	221_L	0.563	0.23
254_A	288_S	0.561	0.22
105_A	206_L	0.561	0.22
303_S	320_I	0.559	0.22
52_F	95_S	0.557	0.22
9_L	13_S	0.557	0.22
159_N	308_Q	0.556	0.22
322_F	325_F	0.555	0.22
131_L	226_G	0.555	0.22
44_V	227_F	0.554	0.22
225_G	307_V	0.553	0.22
155_H	163_G	0.553	0.22
32_A	236_R	0.553	0.22
249_V	252_K	0.552	0.22
53_I	271_G	0.552	0.22
71_L	303_S	0.551	0.22
121_L	167_R	0.551	0.22
356_Q	360_K	0.550	0.22
248_S	253_G	0.550	0.22
356_Q	373_N	0.548	0.22
15_F	29_S	0.548	0.22
211_G	222_L	0.546	0.22
21_T	283_S	0.546	0.22
300_L	333_G	0.545	0.22
126_N	294_I	0.544	0.22
121_L	217_S	0.543	0.22
370_A	383_P	0.543	0.22
372_I	384_A	0.543	0.22
126_N	129_N	0.542	0.22
352_H	362_L	0.541	0.21
37_L	41_W	0.540	0.21
104_T	138_A	0.539	0.21
45_F	129_N	0.538	0.21
159_N	325_F	0.537	0.21
63_I	79_F	0.537	0.21
352_H	359_F	0.537	0.21
128_S	340_V	0.536	0.21
362_L	374_G	0.536	0.21
83_G	86_G	0.534	0.21
373_N	381_I	0.534	0.21
111_N	358_V	0.532	0.21
24_S	188_V	0.532	0.21
133_I	302_F	0.532	0.21
56_E	75_M	0.532	0.21
125_T	321_R	0.532	0.21
63_I	222_L	0.531	0.21
289_L	292_K	0.530	0.21
68_G	76_R	0.530	0.21
298_F	301_G	0.530	0.21
111_N	176_H	0.528	0.21
303_S	379_V	0.527	0.21
231_F	309_A	0.526	0.21
303_S	325_F	0.525	0.21
198_A	302_F	0.525	0.21
45_F	62_G	0.524	0.21
193_H	197_T	0.524	0.21
97_N	228_I	0.522	0.21
245_D	256_T	0.522	0.21
227_F	271_G	0.519	0.20
114_S	214_V	0.518	0.20
24_S	266_I	0.517	0.20
108_R	114_S	0.516	0.20
370_A	373_N	0.516	0.20
227_F	298_F	0.514	0.20
150_L	374_G	0.514	0.20
62_G	68_G	0.511	0.20
198_A	204_R	0.510	0.20
41_W	104_T	0.509	0.20
354_P	357_Y	0.508	0.20
350_A	361_S	0.507	0.20
61_F	141_F	0.506	0.20
250_F	353_D	0.506	0.20
128_S	300_L	0.505	0.20
372_I	382_G	0.505	0.20
247_L	252_K	0.505	0.20
126_N	228_I	0.505	0.20
97_N	236_R	0.503	0.20
53_I	103_L	0.503	0.20
43_V	165_T	0.502	0.20
23_I	69_V	0.501	0.20
359_F	377_L	0.501	0.20
131_L	220_T	0.501	0.20
271_G	299_I	0.500	0.20

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4pd6A	3	0.7549	99	0.887	Contact Map
2cfqA	1	0.8873	13.8	0.974	Contact Map
2ariA	1	0.0686	11.2	0.975	Contact Map
2kv5A	1	0.0809	5.4	0.978	Contact Map
3wdoA	1	0.4755	4.5	0.979	Contact Map
2kncA	1	0.1103	3.9	0.98	Contact Map
4pypA	1	0.3824	3.7	0.98	Contact Map
2l8sA	1	0.1029	3.5	0.98	Contact Map
4he8J	1	0.3603	3.3	0.981	Contact Map
2mc7A	1	0.0735	3.3	0.981	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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