YDJJ - BACSU - GREMLIN Contacts

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YDJJ - Uncharacterized membrane protein YdjJ

UniProt:	O34733	Sequences: MNEGYIIAGLLLLTAGMIDFLWTTLWLESGAGPITRCLSAWLWKGCRKISGDHAKVLSMAGPLLLCLTLVIWISLFWSGWVLIYSSDPHSLMETQSKEPASWSDRIYFSGYVMFTLGNGDLAPNGGLWKLVTIIETAQGLLTITFSVTYLISVLSAVNQKRSFAQSVLSLGHDGTEIVHNAWNGKDFHDIDFLLVAASSELGKLTAQHNAFPILHFYHSTQHQESSIIAVAVLDEALTIFKYGIPEQYQPNQLHIKEARSSIKNYLDTVHTAYIHPAEQAPPEPDISKLQQSGIPALSKQTFQIAVNSIKERRQLLLGIIQAGARKWPVQEQAIGNAYSPK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	341 (314)
Sequences:	246 (208)
Seq/√Len:	11.7

BACSU - YDJJ

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
109_S	135_E	4.594	1.00
158_N	210_A	3.573	0.98
208_H	215_H	3.524	0.98
114_F	137_A	3.501	0.98
145_F	239_I	3.386	0.98
111_Y	118_N	2.953	0.95
46_C	63_L	2.722	0.92
234_D	312_R	2.530	0.89
115_T	144_T	2.432	0.87
160_K	218_H	2.340	0.85
19_D	107_Y	2.331	0.84
104_D	113_M	2.286	0.83
40_A	148_T	2.281	0.83
80_W	164_A	2.247	0.82
10_L	261_S	2.247	0.82
167_V	233_L	2.215	0.81
10_L	134_I	2.156	0.79
131_V	175_T	2.154	0.79
164_A	200_E	2.021	0.74
19_D	30_G	1.972	0.73
112_V	120_D	1.970	0.72
106_I	146_S	1.966	0.72
61_G	120_D	1.943	0.71
80_W	161_R	1.879	0.69
134_I	236_A	1.866	0.68
110_G	149_Y	1.858	0.68
269_V	273_Y	1.831	0.67
107_Y	119_G	1.825	0.66
84_Y	286_I	1.797	0.65
35_T	155_S	1.792	0.65
149_Y	208_H	1.772	0.64
213_I	217_Y	1.772	0.64
20_F	193_L	1.764	0.64
7_I	124_N	1.735	0.62
104_D	123_P	1.722	0.62
61_G	212_P	1.694	0.60
144_T	148_T	1.690	0.60
116_L	131_V	1.686	0.60
16_G	227_I	1.674	0.59
7_I	48_K	1.658	0.59
174_G	218_H	1.654	0.58
66_C	147_V	1.640	0.58
12_L	16_G	1.637	0.58
114_F	163_F	1.632	0.57
24_T	148_T	1.631	0.57
182_W	295_P	1.616	0.57
145_F	206_A	1.613	0.56
42_L	74_S	1.612	0.56
13_L	79_G	1.609	0.56
66_C	202_G	1.604	0.56
112_V	150_L	1.590	0.55
74_S	158_N	1.578	0.55
160_K	265_Y	1.577	0.55
211_F	230_V	1.576	0.55
110_G	116_L	1.563	0.54
108_F	138_Q	1.562	0.54
61_G	211_F	1.562	0.54
113_M	217_Y	1.547	0.53
137_A	205_T	1.543	0.53
34_I	238_T	1.542	0.53
116_L	140_L	1.541	0.53
47_R	172_H	1.533	0.53
206_A	214_L	1.533	0.53
164_A	201_L	1.498	0.51
16_G	76_F	1.498	0.51
24_T	105_R	1.492	0.50
94_T	228_I	1.486	0.50
69_L	142_T	1.482	0.50
41_W	103_S	1.482	0.50
208_H	214_L	1.480	0.50
4_G	240_F	1.479	0.50
156_A	211_F	1.469	0.49
8_A	82_L	1.457	0.49
149_Y	219_S	1.451	0.48
49_I	58_S	1.434	0.48
285_D	309_I	1.433	0.48
19_D	119_G	1.418	0.47
20_F	217_Y	1.410	0.46
116_L	136_T	1.408	0.46
190_I	219_S	1.405	0.46
78_S	277_A	1.403	0.46
143_I	193_L	1.398	0.46
116_L	135_E	1.371	0.45
59_M	123_P	1.360	0.44
207_Q	217_Y	1.358	0.44
20_F	156_A	1.344	0.43
61_G	116_L	1.326	0.42
111_Y	119_G	1.312	0.42
287_S	291_Q	1.309	0.41
15_A	34_I	1.308	0.41
14_T	42_L	1.300	0.41
137_A	141_L	1.296	0.41
149_Y	155_S	1.294	0.41
16_G	222_H	1.293	0.41
20_F	214_L	1.291	0.41
110_G	214_L	1.289	0.41
202_G	217_Y	1.288	0.40
13_L	58_S	1.271	0.40
145_F	208_H	1.268	0.40
131_V	220_T	1.266	0.39
102_W	106_I	1.264	0.39
125_G	240_F	1.262	0.39
111_Y	132_T	1.259	0.39
20_F	101_S	1.257	0.39
72_W	117_G	1.254	0.39
35_T	279_Q	1.251	0.39
114_F	149_Y	1.249	0.39
305_A	313_R	1.227	0.38
147_V	151_I	1.223	0.37
150_L	270_H	1.218	0.37
7_I	70_V	1.214	0.37
306_V	320_I	1.211	0.37
74_S	113_M	1.209	0.37
69_L	229_A	1.206	0.37
112_V	320_I	1.204	0.37
42_L	125_G	1.203	0.36
56_V	113_M	1.200	0.36
77_W	215_H	1.195	0.36
262_I	266_L	1.192	0.36
125_G	186_D	1.186	0.36
61_G	119_G	1.182	0.36
149_Y	212_P	1.179	0.35
235_E	285_D	1.178	0.35
59_M	147_V	1.175	0.35
172_H	225_S	1.171	0.35
10_L	83_I	1.171	0.35
184_G	187_F	1.165	0.35
120_D	129_K	1.163	0.35
38_L	95_Q	1.162	0.35
145_F	214_L	1.160	0.35
41_W	44_K	1.156	0.34
23_T	216_F	1.148	0.34
20_F	66_C	1.147	0.34
285_D	305_A	1.146	0.34
76_F	142_T	1.143	0.34
30_G	75_L	1.140	0.34
234_D	238_T	1.139	0.34
167_V	236_A	1.134	0.33
129_K	135_E	1.133	0.33
57_L	211_F	1.132	0.33
39_S	151_I	1.130	0.33
83_I	153_V	1.127	0.33
19_D	32_G	1.117	0.33
108_F	210_A	1.116	0.33
32_G	194_L	1.108	0.32
240_F	249_Q	1.108	0.32
42_L	294_I	1.103	0.32
85_S	140_L	1.101	0.32
74_S	89_H	1.098	0.32
161_R	226_S	1.097	0.32
51_G	153_V	1.093	0.32
91_L	94_T	1.092	0.32
11_L	90_S	1.082	0.31
23_T	65_L	1.075	0.31
37_C	66_C	1.075	0.31
80_W	135_E	1.068	0.31
78_S	131_V	1.068	0.31
194_L	202_G	1.063	0.30
194_L	225_S	1.060	0.30
123_P	211_F	1.054	0.30
141_L	210_A	1.051	0.30
155_S	177_I	1.050	0.30
32_G	107_Y	1.049	0.30
101_S	125_G	1.048	0.30
154_L	157_V	1.046	0.30
67_L	291_Q	1.039	0.29
171_G	212_P	1.038	0.29
167_V	226_S	1.033	0.29
123_P	150_L	1.030	0.29
154_L	183_N	1.030	0.29
290_Q	296_A	1.026	0.29
63_L	71_I	1.014	0.28
115_T	140_L	1.013	0.28
11_L	267_D	1.010	0.28
156_A	177_I	1.009	0.28
27_L	160_K	1.003	0.28
126_G	129_K	1.001	0.28
43_W	58_S	1.001	0.28
113_M	135_E	1.001	0.28
103_S	176_E	0.997	0.28
120_D	239_I	0.992	0.27
74_S	95_Q	0.988	0.27
21_L	147_V	0.986	0.27
21_L	216_F	0.986	0.27
16_G	75_L	0.982	0.27
140_L	315_L	0.980	0.27
118_N	132_T	0.980	0.27
38_L	41_W	0.980	0.27
108_F	132_T	0.968	0.26
157_V	226_S	0.966	0.26
158_N	268_T	0.966	0.26
149_Y	214_L	0.960	0.26
241_K	252_Q	0.959	0.26
309_I	312_R	0.958	0.26
157_V	219_S	0.957	0.26
24_T	76_F	0.955	0.26
157_V	234_D	0.951	0.26
11_L	221_Q	0.950	0.26
96_S	216_F	0.948	0.26
183_N	186_D	0.948	0.26
26_W	147_V	0.938	0.25
142_T	208_H	0.937	0.25
12_L	49_I	0.929	0.25
12_L	143_I	0.926	0.25
181_A	230_V	0.926	0.25
29_S	33_P	0.925	0.25
154_L	206_A	0.920	0.25
89_H	113_M	0.920	0.25
111_Y	215_H	0.917	0.25
74_S	136_T	0.916	0.25
144_T	163_F	0.914	0.24
38_L	320_I	0.911	0.24
23_T	30_G	0.907	0.24
78_S	106_I	0.907	0.24
109_S	115_T	0.906	0.24
13_L	126_G	0.906	0.24
257_E	261_S	0.906	0.24
313_R	316_L	0.901	0.24
25_L	43_W	0.898	0.24
105_R	160_K	0.897	0.24
116_L	152_S	0.895	0.24
42_L	278_E	0.895	0.24
166_S	264_N	0.894	0.24
121_L	138_Q	0.888	0.24
137_A	163_F	0.884	0.23
105_R	312_R	0.882	0.23
65_L	229_A	0.882	0.23
130_L	267_D	0.882	0.23
38_L	225_S	0.875	0.23
38_L	46_C	0.874	0.23
32_G	43_W	0.869	0.23
72_W	139_G	0.865	0.23
273_Y	294_I	0.864	0.23
15_A	103_S	0.855	0.22
150_L	176_E	0.855	0.22
201_L	230_V	0.854	0.22
176_E	231_A	0.854	0.22
7_I	199_S	0.853	0.22
163_F	201_L	0.848	0.22
177_I	232_V	0.842	0.22
174_G	299_K	0.840	0.22
83_I	229_A	0.836	0.22
99_P	145_F	0.835	0.22
106_I	124_N	0.834	0.22
125_G	199_S	0.833	0.22
108_F	253_L	0.829	0.21
10_L	13_L	0.821	0.21
56_V	319_I	0.819	0.21
69_L	245_P	0.819	0.21
25_L	123_P	0.819	0.21
102_W	152_S	0.817	0.21
292_S	319_I	0.814	0.21
31_A	210_A	0.813	0.21
24_T	233_L	0.812	0.21
270_H	288_K	0.811	0.21
110_G	171_G	0.811	0.21
34_I	94_T	0.808	0.21
141_L	144_T	0.808	0.21
91_L	110_G	0.807	0.21
73_I	261_S	0.806	0.21
69_L	85_S	0.804	0.21
218_H	237_L	0.802	0.21
169_S	225_S	0.799	0.20
52_D	148_T	0.797	0.20
132_T	217_Y	0.795	0.20
140_L	144_T	0.793	0.20
4_G	188_H	0.791	0.20
85_S	138_Q	0.788	0.20
88_P	309_I	0.786	0.20
123_P	270_H	0.785	0.20
69_L	267_D	0.784	0.20
78_S	276_P	0.784	0.20
111_Y	218_H	0.778	0.20
65_L	208_H	0.777	0.20
75_L	225_S	0.776	0.20
256_K	260_S	0.771	0.20
75_L	244_I	0.770	0.20
5_Y	142_T	0.768	0.20
149_Y	213_I	0.767	0.19
148_T	211_F	0.765	0.19
43_W	211_F	0.763	0.19
143_I	299_K	0.762	0.19
281_P	313_R	0.760	0.19
296_A	304_I	0.753	0.19
110_G	212_P	0.752	0.19
145_F	149_Y	0.749	0.19
74_S	188_H	0.742	0.19
218_H	242_Y	0.741	0.19
22_W	235_E	0.741	0.19
4_G	11_L	0.739	0.19
81_V	86_S	0.727	0.18
96_S	229_A	0.725	0.18
150_L	224_E	0.724	0.18
230_V	294_I	0.720	0.18
69_L	276_P	0.717	0.18
113_M	229_A	0.716	0.18
130_L	236_A	0.716	0.18
222_H	227_I	0.715	0.18
44_K	156_A	0.714	0.18
115_T	135_E	0.712	0.18
305_A	312_R	0.712	0.18
104_D	120_D	0.712	0.18
47_R	260_S	0.709	0.18
11_L	78_S	0.708	0.18
83_I	102_W	0.708	0.18
34_I	244_I	0.707	0.18
216_F	225_S	0.707	0.18
139_G	167_V	0.700	0.17
24_T	144_T	0.699	0.17
61_G	139_G	0.694	0.17
92_M	98_E	0.692	0.17
19_D	23_T	0.691	0.17
38_L	269_V	0.691	0.17
154_L	320_I	0.690	0.17
116_L	132_T	0.690	0.17
226_S	230_V	0.690	0.17
49_I	96_S	0.690	0.17
174_G	180_N	0.689	0.17
285_D	312_R	0.685	0.17
81_V	178_V	0.685	0.17
130_L	188_H	0.680	0.17
116_L	142_T	0.677	0.17
139_G	212_P	0.677	0.17
11_L	155_S	0.674	0.17
253_L	257_E	0.673	0.17
81_V	106_I	0.672	0.17
71_I	78_S	0.671	0.17
12_L	103_S	0.671	0.17
243_G	286_I	0.670	0.17
114_F	143_I	0.670	0.17
312_R	320_I	0.667	0.17
9_G	72_W	0.667	0.17
225_S	289_L	0.665	0.16
7_I	60_A	0.665	0.16
317_L	320_I	0.662	0.16
115_T	226_S	0.659	0.16
297_L	307_N	0.653	0.16
17_M	53_H	0.652	0.16
101_S	152_S	0.651	0.16
168_L	263_K	0.651	0.16
10_L	196_A	0.650	0.16
198_S	257_E	0.650	0.16
137_A	142_T	0.646	0.16
112_V	293_G	0.645	0.16
65_L	230_V	0.645	0.16
139_G	214_L	0.645	0.16
21_L	264_N	0.644	0.16
66_C	188_H	0.644	0.16
107_Y	140_L	0.644	0.16
58_S	120_D	0.644	0.16
109_S	140_L	0.643	0.16
19_D	43_W	0.643	0.16
222_H	283_E	0.642	0.16
4_G	56_V	0.640	0.16
64_L	69_L	0.640	0.16
10_L	48_K	0.640	0.16
43_W	61_G	0.638	0.16
119_G	164_A	0.637	0.16
177_I	292_S	0.635	0.16
88_P	249_Q	0.633	0.16
62_P	217_Y	0.631	0.16
12_L	279_Q	0.630	0.16
235_E	239_I	0.628	0.16
62_P	147_V	0.627	0.15
14_T	179_H	0.625	0.15
156_A	208_H	0.625	0.15
113_M	123_P	0.624	0.15
95_Q	120_D	0.622	0.15
119_G	139_G	0.621	0.15
176_E	199_S	0.620	0.15
52_D	226_S	0.620	0.15
7_I	247_Q	0.618	0.15
12_L	78_S	0.617	0.15
150_L	174_G	0.611	0.15
34_I	134_I	0.611	0.15
22_W	26_W	0.610	0.15
17_M	249_Q	0.607	0.15
91_L	193_L	0.606	0.15
226_S	258_A	0.606	0.15
293_G	296_A	0.605	0.15
240_F	298_S	0.605	0.15
185_K	313_R	0.604	0.15
295_P	300_Q	0.603	0.15
136_T	305_A	0.603	0.15
11_L	133_I	0.603	0.15
121_L	210_A	0.603	0.15
14_T	130_L	0.600	0.15
139_G	156_A	0.598	0.15
10_L	199_S	0.597	0.15
104_D	219_S	0.593	0.15
78_S	252_Q	0.587	0.14
51_G	283_E	0.587	0.14
118_N	122_A	0.585	0.14
304_I	308_S	0.585	0.14
291_Q	307_N	0.583	0.14
83_I	280_A	0.580	0.14
66_C	86_S	0.579	0.14
78_S	267_D	0.578	0.14
18_I	66_C	0.577	0.14
117_G	139_G	0.576	0.14
65_L	244_I	0.575	0.14
86_S	171_G	0.571	0.14
137_A	143_I	0.571	0.14
10_L	217_Y	0.570	0.14
45_G	229_A	0.569	0.14
245_P	280_A	0.569	0.14
77_W	171_G	0.568	0.14
54_A	152_S	0.568	0.14
127_L	277_A	0.567	0.14
160_K	317_L	0.565	0.14
92_M	96_S	0.565	0.14
285_D	289_L	0.564	0.14
112_V	119_G	0.563	0.14
151_I	164_A	0.557	0.14
86_S	169_S	0.557	0.14
170_L	197_A	0.557	0.14
91_L	149_Y	0.557	0.14
19_D	196_A	0.557	0.14
73_I	256_K	0.557	0.14
132_T	205_T	0.556	0.14
94_T	200_E	0.555	0.14
19_D	146_S	0.555	0.14
76_F	212_P	0.555	0.14
230_V	283_E	0.551	0.14
4_G	13_L	0.550	0.14
126_G	231_A	0.550	0.14
137_A	157_V	0.548	0.14
137_A	149_Y	0.548	0.14
268_T	276_P	0.548	0.14
190_I	206_A	0.543	0.13
53_H	109_S	0.541	0.13
61_G	123_P	0.538	0.13
84_Y	108_F	0.538	0.13
46_C	60_A	0.534	0.13
31_A	39_S	0.533	0.13
19_D	282_P	0.533	0.13
170_L	233_L	0.532	0.13
84_Y	109_S	0.528	0.13
63_L	264_N	0.527	0.13
152_S	217_Y	0.525	0.13
20_F	229_A	0.525	0.13
16_G	217_Y	0.524	0.13
52_D	178_V	0.523	0.13
31_A	116_L	0.522	0.13
109_S	154_L	0.521	0.13
10_L	131_V	0.521	0.13
35_T	116_L	0.520	0.13
141_L	216_F	0.520	0.13
9_G	25_L	0.519	0.13
293_G	304_I	0.515	0.13
104_D	110_G	0.514	0.13
207_Q	234_D	0.513	0.13
185_K	290_Q	0.511	0.13
80_W	207_Q	0.511	0.13
53_H	73_I	0.509	0.13
62_P	66_C	0.507	0.13
124_N	263_K	0.506	0.13
139_G	160_K	0.505	0.13
73_I	148_T	0.505	0.13
68_T	205_T	0.505	0.13
80_W	115_T	0.504	0.13
76_F	138_Q	0.504	0.13
7_I	222_H	0.503	0.13
23_T	119_G	0.503	0.13
115_T	139_G	0.503	0.13
151_I	241_K	0.502	0.13
136_T	142_T	0.501	0.12
86_S	190_I	0.501	0.12
52_D	288_K	0.500	0.12
3_E	86_S	0.500	0.12
23_T	43_W	0.500	0.12

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3effK	4	0.4076	99.2	0.868	Contact Map
4wfeA	2	0.393	99.1	0.873	Contact Map
4twkA	2	0.4135	99.1	0.874	Contact Map
3pjsK	4	0.4076	99.1	0.874	Contact Map
4h33A	3	0.2669	99.1	0.877	Contact Map
2ih3C	4	0.2845	99	0.882	Contact Map
3vouA	4	0.4018	99	0.883	Contact Map
4bw5A	2	0.4018	98.9	0.886	Contact Map
1p7bA	3	0.7067	98.8	0.888	Contact Map
3ukmA	2	0.3988	98.7	0.891	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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