GREMLIN Database
RL32 - 50S ribosomal protein L32
UniProt: O34687 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 59 (56)
Sequences: 759 (345)
Seq/√Len: 46.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
32_S48_S3.7071.00
3_V8_T3.1101.00
35_E45_A2.8791.00
27_M36_M2.7601.00
29_E34_G2.3671.00
8_T13_K2.1241.00
29_E36_M1.9760.99
11_M15_L1.8860.99
15_L18_T1.7990.99
12_K15_L1.7710.98
26_G39_S1.7490.98
38_L41_R1.6510.98
11_M14_R1.6050.97
27_M39_S1.5310.96
53_D56_V1.5220.96
18_T21_K1.4180.94
13_K19_H1.3080.91
2_A8_T1.2020.87
31_P34_G1.1520.85
8_T20_F1.1190.83
12_K22_L1.0510.79
7_R11_M0.9990.75
31_P37_K0.9980.75
31_P48_S0.9940.75
2_A34_G0.9940.75
5_F20_F0.9730.73
24_V39_S0.9670.73
5_F11_M0.9620.72
7_R13_K0.9500.72
37_K50_N0.9430.71
22_L26_G0.9020.67
43_C46_C0.8910.67
33_C46_C0.8910.67
33_C43_C0.8910.67
7_R23_N0.8790.65
8_T12_K0.8490.63
10_K14_R0.8460.62
10_K13_K0.8290.61
22_L41_R0.8240.60
3_V14_R0.8170.60
28_T50_N0.7810.56
23_N48_S0.7750.56
38_L52_K0.7610.54
35_E54_I0.7560.54
9_S12_K0.7430.53
25_P32_S0.7320.52
42_V47_G0.7150.50
37_K45_A0.7090.49
8_T16_R0.7040.49
2_A51_G0.6920.48
26_G50_N0.6770.46
8_T22_L0.6680.45
37_K44_K0.6540.44
32_S44_K0.6450.43
41_R48_S0.6390.42
1_M37_K0.6370.42
34_G42_V0.6340.42
38_L44_K0.6260.41
3_V16_R0.6090.40
23_N50_N0.6070.39
26_G29_E0.6060.39
2_A7_R0.6050.39
26_G52_K0.5930.38
8_T19_H0.5850.37
44_K48_S0.5770.37
6_R20_F0.5730.36
28_T37_K0.5710.36
25_P29_E0.5650.36
2_A19_H0.5640.35
11_M18_T0.5620.35
47_G54_I0.5610.35
29_E45_A0.5590.35
34_G51_G0.5510.34
28_T34_G0.5400.33
42_V50_N0.5350.33
12_K20_F0.5230.32
50_N53_D0.5190.31
24_V27_M0.5190.31
24_V51_G0.5140.31
14_R17_R0.5130.31
17_R50_N0.5090.31
16_R19_H0.5070.30
45_A55_N0.5000.30
35_E48_S0.5000.30
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3j3w0 1 0.9322 99.9 0.167 Contact Map
2zjrZ 1 0.9322 99.9 0.178 Contact Map
4rb65 1 0.9661 99.9 0.186 Contact Map
4tp90 1 0.9322 99.9 0.239 Contact Map
3j7y0 1 0.9831 99.9 0.268 Contact Map
4ce45 1 0.7627 99.8 0.328 Contact Map
1vw4W 1 0.9661 99.8 0.347 Contact Map
3bbo2 1 0.8475 98.8 0.619 Contact Map
4c2mI 1 0.6271 73 0.854 Contact Map
3o9xA 2 0.5593 72.8 0.854 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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